Suppression of methionine-induced colon injury of young rats by cysteine and N-acetyl-l-cysteine
Samo za registrovane korisnike
2018
Autori
Stojanović, MarijaŠćepanović, Ljiljana
Todorović, Dusan
Mitrović, Dušan
Šćepanović, Vuk
Scepanović, Radomir
Ilić, Slobodan
Šćepanović, Teja
Labudović-Borović, Milica
Milicević, Zivana
Dragutinović, Vesna
Borozan, Sunčica
Lalić, Ivana
Despotović, Sanja
Đurić, Dragan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Changes in the methionine metabolism can cause a state called hyperhomocysteinemia, inducing oxidative stress in the gut. The production of free radicals is important in the colon damage caused by methionine. This study aimed at evaluating the effect of the use of l-cysteine and N-acetyl-l-cysteine on the colon morphometry of young rats treated with methionine. A total number of 32 male rats were distributed in a randomized experimental design in 4 groups: control group treated with saline; methionine group; cysteine + methionine group, and N-acetyl-l-cysteine + methionine group. After 21 days of treatment, rats were sacrificed and the colon samples were taken for histological and biochemical analysis. Methionine load increased depth of crypts, the lamina muscularis mucosae thickness, the mucosal height, and the number of cells in lamina propria (p < 0.01). Combination of methionine with l-cysteine (C group) and with N-acetyl-l-cysteine (N group) reversed methionine effects. Methionine... treatment increased the GPx activity and MDA concentration, while l-cysteine and N-acetyl-l-cysteine increased the catalase activity compared to methionine group. It was concluded that the use of l-cysteine and N-acetyl-l-cysteine was beneficial to decrease intestinal mucosal height and oxidative damage when methionine was used in combination with them.
Ključne reči:
Methionine / Colon / Morphometry / Oxidative stress / RatIzvor:
Molecular and Cellular Biochemistry, 2018, 440, 1-2, 53-64Izdavač:
- Springer, Dordrecht
Finansiranje / projekti:
- Efekti homocisteina i homocisteinu srodnih supstanci na kardiovaskularni sistem: Uloga gasnih transmitera NO, H2S I CO (RS-MESTD-Basic Research (BR or ON)-175043)
DOI: 10.1007/s11010-017-3155-1
ISSN: 0300-8177
PubMed: 28819915
WoS: 000425132100006
Scopus: 2-s2.0-85027724485
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Stojanović, Marija AU - Šćepanović, Ljiljana AU - Todorović, Dusan AU - Mitrović, Dušan AU - Šćepanović, Vuk AU - Scepanović, Radomir AU - Ilić, Slobodan AU - Šćepanović, Teja AU - Labudović-Borović, Milica AU - Milicević, Zivana AU - Dragutinović, Vesna AU - Borozan, Sunčica AU - Lalić, Ivana AU - Despotović, Sanja AU - Đurić, Dragan PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1662 AB - Changes in the methionine metabolism can cause a state called hyperhomocysteinemia, inducing oxidative stress in the gut. The production of free radicals is important in the colon damage caused by methionine. This study aimed at evaluating the effect of the use of l-cysteine and N-acetyl-l-cysteine on the colon morphometry of young rats treated with methionine. A total number of 32 male rats were distributed in a randomized experimental design in 4 groups: control group treated with saline; methionine group; cysteine + methionine group, and N-acetyl-l-cysteine + methionine group. After 21 days of treatment, rats were sacrificed and the colon samples were taken for histological and biochemical analysis. Methionine load increased depth of crypts, the lamina muscularis mucosae thickness, the mucosal height, and the number of cells in lamina propria (p < 0.01). Combination of methionine with l-cysteine (C group) and with N-acetyl-l-cysteine (N group) reversed methionine effects. Methionine treatment increased the GPx activity and MDA concentration, while l-cysteine and N-acetyl-l-cysteine increased the catalase activity compared to methionine group. It was concluded that the use of l-cysteine and N-acetyl-l-cysteine was beneficial to decrease intestinal mucosal height and oxidative damage when methionine was used in combination with them. PB - Springer, Dordrecht T2 - Molecular and Cellular Biochemistry T1 - Suppression of methionine-induced colon injury of young rats by cysteine and N-acetyl-l-cysteine VL - 440 IS - 1-2 SP - 53 EP - 64 DO - 10.1007/s11010-017-3155-1 ER -
@article{ author = "Stojanović, Marija and Šćepanović, Ljiljana and Todorović, Dusan and Mitrović, Dušan and Šćepanović, Vuk and Scepanović, Radomir and Ilić, Slobodan and Šćepanović, Teja and Labudović-Borović, Milica and Milicević, Zivana and Dragutinović, Vesna and Borozan, Sunčica and Lalić, Ivana and Despotović, Sanja and Đurić, Dragan", year = "2018", abstract = "Changes in the methionine metabolism can cause a state called hyperhomocysteinemia, inducing oxidative stress in the gut. The production of free radicals is important in the colon damage caused by methionine. This study aimed at evaluating the effect of the use of l-cysteine and N-acetyl-l-cysteine on the colon morphometry of young rats treated with methionine. A total number of 32 male rats were distributed in a randomized experimental design in 4 groups: control group treated with saline; methionine group; cysteine + methionine group, and N-acetyl-l-cysteine + methionine group. After 21 days of treatment, rats were sacrificed and the colon samples were taken for histological and biochemical analysis. Methionine load increased depth of crypts, the lamina muscularis mucosae thickness, the mucosal height, and the number of cells in lamina propria (p < 0.01). Combination of methionine with l-cysteine (C group) and with N-acetyl-l-cysteine (N group) reversed methionine effects. Methionine treatment increased the GPx activity and MDA concentration, while l-cysteine and N-acetyl-l-cysteine increased the catalase activity compared to methionine group. It was concluded that the use of l-cysteine and N-acetyl-l-cysteine was beneficial to decrease intestinal mucosal height and oxidative damage when methionine was used in combination with them.", publisher = "Springer, Dordrecht", journal = "Molecular and Cellular Biochemistry", title = "Suppression of methionine-induced colon injury of young rats by cysteine and N-acetyl-l-cysteine", volume = "440", number = "1-2", pages = "53-64", doi = "10.1007/s11010-017-3155-1" }
Stojanović, M., Šćepanović, L., Todorović, D., Mitrović, D., Šćepanović, V., Scepanović, R., Ilić, S., Šćepanović, T., Labudović-Borović, M., Milicević, Z., Dragutinović, V., Borozan, S., Lalić, I., Despotović, S.,& Đurić, D.. (2018). Suppression of methionine-induced colon injury of young rats by cysteine and N-acetyl-l-cysteine. in Molecular and Cellular Biochemistry Springer, Dordrecht., 440(1-2), 53-64. https://doi.org/10.1007/s11010-017-3155-1
Stojanović M, Šćepanović L, Todorović D, Mitrović D, Šćepanović V, Scepanović R, Ilić S, Šćepanović T, Labudović-Borović M, Milicević Z, Dragutinović V, Borozan S, Lalić I, Despotović S, Đurić D. Suppression of methionine-induced colon injury of young rats by cysteine and N-acetyl-l-cysteine. in Molecular and Cellular Biochemistry. 2018;440(1-2):53-64. doi:10.1007/s11010-017-3155-1 .
Stojanović, Marija, Šćepanović, Ljiljana, Todorović, Dusan, Mitrović, Dušan, Šćepanović, Vuk, Scepanović, Radomir, Ilić, Slobodan, Šćepanović, Teja, Labudović-Borović, Milica, Milicević, Zivana, Dragutinović, Vesna, Borozan, Sunčica, Lalić, Ivana, Despotović, Sanja, Đurić, Dragan, "Suppression of methionine-induced colon injury of young rats by cysteine and N-acetyl-l-cysteine" in Molecular and Cellular Biochemistry, 440, no. 1-2 (2018):53-64, https://doi.org/10.1007/s11010-017-3155-1 . .