Clostridium botulinum spores in European honey bees from Serbia
Само за регистроване кориснике
2019
Аутори
Matović, KazimirMišić, Dušan
Karabasil, Nedjeljko
Nedić, Nebojša
Dmitrić, Marko
Jevtić, Goran
Ćirić, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A total of 61 honey bees from different regions of the Republic of Serbia were analyzed for Clostridium botulinum (C. botulinum) spores. The microbiological methods and molecular methods (multiplex PCR/mPCR and PCR method) were utilized to examine multiple subunits of each honey bees samples. The C. botulinum spores in PCR-positive samples were estimated by the most probable number method (MPN). The presence of C. botulinum spores, by applying mPCR and PCR methods, was detected in 1 of the 61 honey bees (1.64%). Using MPN method, the number of spores in positive sample was 110/kg. Detection of C. botulinum spores directly from untreated honey bees, without prior enrichment, is impossible by applying PCR. Using conventional microbiological methods, detection of C. botulinum spores in dead honey bees is not possible without preenrichment. Therefore, conventional, microbiological methods are not suitable for the detection of C. botulinum spores in honey bees. In order to detect C. botulin...um spores in honey bees using PCR methods, due to the small and/or unequal distribution of spores in the samples, it is desirable to use multiple subunits/replicates for each sample examined.
Кључне речи:
Apis mellifera / Clostridium botulinum / microbiological methods / PCRИзвор:
Journal of Apicultural Research, 2019, 58, 3, 420-426Издавач:
- Taylor & Francis Ltd, Abingdon
Финансирање / пројекти:
- Развој и стандардизација молекуларних и имунских метода у дијагностици бактеријских зооноза (RS-MESTD-Technological Development (TD or TR)-31088)
- Побољшање генетичког потенцијала и технологија производње крмног биља у функцији одрживог развоја сточарства (RS-MESTD-Technological Development (TD or TR)-31057)
DOI: 10.1080/00218839.2018.1560654
ISSN: 0021-8839
WoS: 000466809300004
Scopus: 2-s2.0-85061025323
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Matović, Kazimir AU - Mišić, Dušan AU - Karabasil, Nedjeljko AU - Nedić, Nebojša AU - Dmitrić, Marko AU - Jevtić, Goran AU - Ćirić, Jelena PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1756 AB - A total of 61 honey bees from different regions of the Republic of Serbia were analyzed for Clostridium botulinum (C. botulinum) spores. The microbiological methods and molecular methods (multiplex PCR/mPCR and PCR method) were utilized to examine multiple subunits of each honey bees samples. The C. botulinum spores in PCR-positive samples were estimated by the most probable number method (MPN). The presence of C. botulinum spores, by applying mPCR and PCR methods, was detected in 1 of the 61 honey bees (1.64%). Using MPN method, the number of spores in positive sample was 110/kg. Detection of C. botulinum spores directly from untreated honey bees, without prior enrichment, is impossible by applying PCR. Using conventional microbiological methods, detection of C. botulinum spores in dead honey bees is not possible without preenrichment. Therefore, conventional, microbiological methods are not suitable for the detection of C. botulinum spores in honey bees. In order to detect C. botulinum spores in honey bees using PCR methods, due to the small and/or unequal distribution of spores in the samples, it is desirable to use multiple subunits/replicates for each sample examined. PB - Taylor & Francis Ltd, Abingdon T2 - Journal of Apicultural Research T1 - Clostridium botulinum spores in European honey bees from Serbia VL - 58 IS - 3 SP - 420 EP - 426 DO - 10.1080/00218839.2018.1560654 ER -
@article{ author = "Matović, Kazimir and Mišić, Dušan and Karabasil, Nedjeljko and Nedić, Nebojša and Dmitrić, Marko and Jevtić, Goran and Ćirić, Jelena", year = "2019", abstract = "A total of 61 honey bees from different regions of the Republic of Serbia were analyzed for Clostridium botulinum (C. botulinum) spores. The microbiological methods and molecular methods (multiplex PCR/mPCR and PCR method) were utilized to examine multiple subunits of each honey bees samples. The C. botulinum spores in PCR-positive samples were estimated by the most probable number method (MPN). The presence of C. botulinum spores, by applying mPCR and PCR methods, was detected in 1 of the 61 honey bees (1.64%). Using MPN method, the number of spores in positive sample was 110/kg. Detection of C. botulinum spores directly from untreated honey bees, without prior enrichment, is impossible by applying PCR. Using conventional microbiological methods, detection of C. botulinum spores in dead honey bees is not possible without preenrichment. Therefore, conventional, microbiological methods are not suitable for the detection of C. botulinum spores in honey bees. In order to detect C. botulinum spores in honey bees using PCR methods, due to the small and/or unequal distribution of spores in the samples, it is desirable to use multiple subunits/replicates for each sample examined.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Journal of Apicultural Research", title = "Clostridium botulinum spores in European honey bees from Serbia", volume = "58", number = "3", pages = "420-426", doi = "10.1080/00218839.2018.1560654" }
Matović, K., Mišić, D., Karabasil, N., Nedić, N., Dmitrić, M., Jevtić, G.,& Ćirić, J.. (2019). Clostridium botulinum spores in European honey bees from Serbia. in Journal of Apicultural Research Taylor & Francis Ltd, Abingdon., 58(3), 420-426. https://doi.org/10.1080/00218839.2018.1560654
Matović K, Mišić D, Karabasil N, Nedić N, Dmitrić M, Jevtić G, Ćirić J. Clostridium botulinum spores in European honey bees from Serbia. in Journal of Apicultural Research. 2019;58(3):420-426. doi:10.1080/00218839.2018.1560654 .
Matović, Kazimir, Mišić, Dušan, Karabasil, Nedjeljko, Nedić, Nebojša, Dmitrić, Marko, Jevtić, Goran, Ćirić, Jelena, "Clostridium botulinum spores in European honey bees from Serbia" in Journal of Apicultural Research, 58, no. 3 (2019):420-426, https://doi.org/10.1080/00218839.2018.1560654 . .