Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties
Authorized Users Only
2019
Authors
Kosanović, MajaCvetković, Jelena
Gruden-Movsesijan, Alisa
Vasilev, Saša
Milanović, Svetlana
Ilić, Natasa
Sofronic-Milosavljević, Ljiljana
Article (Published version)
Metadata
Show full item recordAbstract
Aims Extracellular vesicles (EVs) represent a newly discovered but universal communication tool between cells or organisms. However, few data exist on nematode EVs and none for Trichinella spiralis. Here, we aimed to investigate whether T spiralis muscle larvae produce EVs, whether they carry immunomodulatory proteins and whether they have a role in immunomodulation as a component of excretory-secretory muscle larvae products (ES L1). Methods and results EVs were enriched from conditioned medium of T spiralis muscle larvae. Transmission electron microscopy images showed T spiralis EVs to be 30-80 nm in size, and Western blot confirmed the presence of two out of three glycoproteins with the immunodominant epitope characteristic for muscle larvae of the genus Trichinella. Using a peripheral blood mononuclear cell (PBMC) stimulation assay, it was shown that these EVs elevated production of IL10 and IL6. Conclusion T spiralis muscle larvae produce EVs. Those EVs carry immunomodulatory prot...eins and have the capacity independently to induce regulatory responses in the same way as the T spiralis excretory-secretory muscle larvae products from which they were isolated.
Keywords:
ES L1 / exosomes / Helminths / immunomodulation / muscle larvae / nematodeSource:
Parasite Immunology, 2019, 41, 10, e12665-Publisher:
- Wiley, Hoboken
Funding / projects:
- Mechanisms of the immune response to parasitic infection, or exposure to parasite antigens, and the concomitant modulation and/or prevention of unrelated diseases (RS-173047)
- Application of functionalyzed carbon nanotubes and gold nanoparticles for preparation of dendritic cells for tumor therapy (RS-175102)
DOI: 10.1111/pim.12665
ISSN: 0141-9838
PubMed: 31356691
WoS: 000485468400001
Scopus: 2-s2.0-85072234758
Collections
Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Kosanović, Maja AU - Cvetković, Jelena AU - Gruden-Movsesijan, Alisa AU - Vasilev, Saša AU - Milanović, Svetlana AU - Ilić, Natasa AU - Sofronic-Milosavljević, Ljiljana PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1785 AB - Aims Extracellular vesicles (EVs) represent a newly discovered but universal communication tool between cells or organisms. However, few data exist on nematode EVs and none for Trichinella spiralis. Here, we aimed to investigate whether T spiralis muscle larvae produce EVs, whether they carry immunomodulatory proteins and whether they have a role in immunomodulation as a component of excretory-secretory muscle larvae products (ES L1). Methods and results EVs were enriched from conditioned medium of T spiralis muscle larvae. Transmission electron microscopy images showed T spiralis EVs to be 30-80 nm in size, and Western blot confirmed the presence of two out of three glycoproteins with the immunodominant epitope characteristic for muscle larvae of the genus Trichinella. Using a peripheral blood mononuclear cell (PBMC) stimulation assay, it was shown that these EVs elevated production of IL10 and IL6. Conclusion T spiralis muscle larvae produce EVs. Those EVs carry immunomodulatory proteins and have the capacity independently to induce regulatory responses in the same way as the T spiralis excretory-secretory muscle larvae products from which they were isolated. PB - Wiley, Hoboken T2 - Parasite Immunology T1 - Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties VL - 41 IS - 10 SP - e12665 DO - 10.1111/pim.12665 ER -
@article{ author = "Kosanović, Maja and Cvetković, Jelena and Gruden-Movsesijan, Alisa and Vasilev, Saša and Milanović, Svetlana and Ilić, Natasa and Sofronic-Milosavljević, Ljiljana", year = "2019", abstract = "Aims Extracellular vesicles (EVs) represent a newly discovered but universal communication tool between cells or organisms. However, few data exist on nematode EVs and none for Trichinella spiralis. Here, we aimed to investigate whether T spiralis muscle larvae produce EVs, whether they carry immunomodulatory proteins and whether they have a role in immunomodulation as a component of excretory-secretory muscle larvae products (ES L1). Methods and results EVs were enriched from conditioned medium of T spiralis muscle larvae. Transmission electron microscopy images showed T spiralis EVs to be 30-80 nm in size, and Western blot confirmed the presence of two out of three glycoproteins with the immunodominant epitope characteristic for muscle larvae of the genus Trichinella. Using a peripheral blood mononuclear cell (PBMC) stimulation assay, it was shown that these EVs elevated production of IL10 and IL6. Conclusion T spiralis muscle larvae produce EVs. Those EVs carry immunomodulatory proteins and have the capacity independently to induce regulatory responses in the same way as the T spiralis excretory-secretory muscle larvae products from which they were isolated.", publisher = "Wiley, Hoboken", journal = "Parasite Immunology", title = "Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties", volume = "41", number = "10", pages = "e12665", doi = "10.1111/pim.12665" }
Kosanović, M., Cvetković, J., Gruden-Movsesijan, A., Vasilev, S., Milanović, S., Ilić, N.,& Sofronic-Milosavljević, L.. (2019). Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties. in Parasite Immunology Wiley, Hoboken., 41(10), e12665. https://doi.org/10.1111/pim.12665
Kosanović M, Cvetković J, Gruden-Movsesijan A, Vasilev S, Milanović S, Ilić N, Sofronic-Milosavljević L. Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties. in Parasite Immunology. 2019;41(10):e12665. doi:10.1111/pim.12665 .
Kosanović, Maja, Cvetković, Jelena, Gruden-Movsesijan, Alisa, Vasilev, Saša, Milanović, Svetlana, Ilić, Natasa, Sofronic-Milosavljević, Ljiljana, "Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties" in Parasite Immunology, 41, no. 10 (2019):e12665, https://doi.org/10.1111/pim.12665 . .