Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia
2020
Аутори
Žutić, JadrankaValčić, Olivera
Milićević, Vesna
Veljović, Ljubiša
Kureljušić, Jasna
Bacić, Dragan
Jezdimirović, Nemanja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A total of 120 Escherichia coli (E. coli) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrial and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (33.33%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrial and toxin genes were found. The most common virulence pattern was F4/STb/LT/EAST1 detec...ted in 23.45% of E. coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from diarrhoeic suckling piglets in Serbia.
Кључне речи:
Escherichia coli / adhesin / enterotoxin / virulence genes / diarrhoeaИзвор:
Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice, 2020, 76, 1, 41-45Издавач:
- Polish Soc Veterinary Sciences Editorial Office, Lublin
Финансирање / пројекти:
- Усавршавање припреме вакцина са неким врстама бактерија ради превенирања економски значајних цревних и респираторних инфекција код прасади-елиминација могућности преношења гена резистенције на антибиотике путем аутохтоних сојева укомпонованих у вакцине (RS-MESTD-MPN2006-2010-20151)
DOI: 10.21521/mw.6326
ISSN: 0025-8628
WoS: 000503039500007
Scopus: 2-s2.0-85095712413
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Žutić, Jadranka AU - Valčić, Olivera AU - Milićević, Vesna AU - Veljović, Ljubiša AU - Kureljušić, Jasna AU - Bacić, Dragan AU - Jezdimirović, Nemanja PY - 2020 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1796 AB - A total of 120 Escherichia coli (E. coli) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrial and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (33.33%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrial and toxin genes were found. The most common virulence pattern was F4/STb/LT/EAST1 detected in 23.45% of E. coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from diarrhoeic suckling piglets in Serbia. PB - Polish Soc Veterinary Sciences Editorial Office, Lublin T2 - Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice T1 - Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia VL - 76 IS - 1 SP - 41 EP - 45 DO - 10.21521/mw.6326 ER -
@article{ author = "Žutić, Jadranka and Valčić, Olivera and Milićević, Vesna and Veljović, Ljubiša and Kureljušić, Jasna and Bacić, Dragan and Jezdimirović, Nemanja", year = "2020", abstract = "A total of 120 Escherichia coli (E. coli) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrial and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (33.33%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrial and toxin genes were found. The most common virulence pattern was F4/STb/LT/EAST1 detected in 23.45% of E. coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from diarrhoeic suckling piglets in Serbia.", publisher = "Polish Soc Veterinary Sciences Editorial Office, Lublin", journal = "Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice", title = "Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia", volume = "76", number = "1", pages = "41-45", doi = "10.21521/mw.6326" }
Žutić, J., Valčić, O., Milićević, V., Veljović, L., Kureljušić, J., Bacić, D.,& Jezdimirović, N.. (2020). Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice Polish Soc Veterinary Sciences Editorial Office, Lublin., 76(1), 41-45. https://doi.org/10.21521/mw.6326
Žutić J, Valčić O, Milićević V, Veljović L, Kureljušić J, Bacić D, Jezdimirović N. Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice. 2020;76(1):41-45. doi:10.21521/mw.6326 .
Žutić, Jadranka, Valčić, Olivera, Milićević, Vesna, Veljović, Ljubiša, Kureljušić, Jasna, Bacić, Dragan, Jezdimirović, Nemanja, "Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia" in Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice, 76, no. 1 (2020):41-45, https://doi.org/10.21521/mw.6326 . .