Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction
Samo za registrovane korisnike
2020
Autori
Micić, M.Antonijević, D.
Milutinović-Smiljanić, Sanja
Trisić, D.
Colović, B.
Kosanović, D.
Prokić, B.
Vasić, Jugoslav
Živković, Slavoljub
Milasin, J.
Danilović, V.
Đurić, M.
Jokanović, Vukoman
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aim of this study was to develop novel hydroxyapatite (HAP)-based bioactive bone replacement materials for segmental osteotomy reconstruction. Customized three-dimensional (3D) bone construct was manufactured from nanohydroxyapatite (nHAP) with poly(lactide-co-glycolide) (PLGA) coating using 3D models derived from the computed tomography (CT) scanning of the rabbit's ulna and gradient 3D printing of the bone substitute mimicking the anatomical shape of the natural bone defect. Engineered construct revealed adequate micro-architectural design for successful bone regeneration having a total porosity of 64% and an average pore size of 256 μm. Radiography and micro-CT analysis depicted new bone apposition through the whole length of the reconstructed ulna with a small area of non-resorbed construct in the central area of defect. Histological analysis revealed new bone formation with both endochondral and endesmal type of ossification. Immunohistochemistry analysis depicted the presence... of bone formation indicators-bone morphogenetic protein (BMP), osteocalcin (OCN) and osteopontin (OPN) within newly formed bone. Manufactured personalized construct acts as a "smart" responsive biomaterial capable of modulating the functionality and potential for the personalized bone reconstruction on a clinically relevant length scale.
Ključne reči:
3D printing / bone reconstruction / personalized medicine / tissue engineeringIzvor:
Biomedizinische Technik, 2020Izdavač:
- De Gruyter
Finansiranje / projekti:
- Hemijsko i strukturno dizajniranje nanomaterijala za primenu u medicini i inženjerstvu tkiva (RS-MESTD-Basic Research (BR or ON)-172026)
- Funkcionalni, funkcionalizovani i usavršeni nano materijali (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45005)
DOI: 10.1515/bmt-2019-0218
ISSN: 0013-5585
PubMed: 32335536
WoS: 000560600600012
Scopus: 2-s2.0-85084702256
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Micić, M. AU - Antonijević, D. AU - Milutinović-Smiljanić, Sanja AU - Trisić, D. AU - Colović, B. AU - Kosanović, D. AU - Prokić, B. AU - Vasić, Jugoslav AU - Živković, Slavoljub AU - Milasin, J. AU - Danilović, V. AU - Đurić, M. AU - Jokanović, Vukoman PY - 2020 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1811 AB - The aim of this study was to develop novel hydroxyapatite (HAP)-based bioactive bone replacement materials for segmental osteotomy reconstruction. Customized three-dimensional (3D) bone construct was manufactured from nanohydroxyapatite (nHAP) with poly(lactide-co-glycolide) (PLGA) coating using 3D models derived from the computed tomography (CT) scanning of the rabbit's ulna and gradient 3D printing of the bone substitute mimicking the anatomical shape of the natural bone defect. Engineered construct revealed adequate micro-architectural design for successful bone regeneration having a total porosity of 64% and an average pore size of 256 μm. Radiography and micro-CT analysis depicted new bone apposition through the whole length of the reconstructed ulna with a small area of non-resorbed construct in the central area of defect. Histological analysis revealed new bone formation with both endochondral and endesmal type of ossification. Immunohistochemistry analysis depicted the presence of bone formation indicators-bone morphogenetic protein (BMP), osteocalcin (OCN) and osteopontin (OPN) within newly formed bone. Manufactured personalized construct acts as a "smart" responsive biomaterial capable of modulating the functionality and potential for the personalized bone reconstruction on a clinically relevant length scale. PB - De Gruyter T2 - Biomedizinische Technik T1 - Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction DO - 10.1515/bmt-2019-0218 ER -
@article{ author = "Micić, M. and Antonijević, D. and Milutinović-Smiljanić, Sanja and Trisić, D. and Colović, B. and Kosanović, D. and Prokić, B. and Vasić, Jugoslav and Živković, Slavoljub and Milasin, J. and Danilović, V. and Đurić, M. and Jokanović, Vukoman", year = "2020", abstract = "The aim of this study was to develop novel hydroxyapatite (HAP)-based bioactive bone replacement materials for segmental osteotomy reconstruction. Customized three-dimensional (3D) bone construct was manufactured from nanohydroxyapatite (nHAP) with poly(lactide-co-glycolide) (PLGA) coating using 3D models derived from the computed tomography (CT) scanning of the rabbit's ulna and gradient 3D printing of the bone substitute mimicking the anatomical shape of the natural bone defect. Engineered construct revealed adequate micro-architectural design for successful bone regeneration having a total porosity of 64% and an average pore size of 256 μm. Radiography and micro-CT analysis depicted new bone apposition through the whole length of the reconstructed ulna with a small area of non-resorbed construct in the central area of defect. Histological analysis revealed new bone formation with both endochondral and endesmal type of ossification. Immunohistochemistry analysis depicted the presence of bone formation indicators-bone morphogenetic protein (BMP), osteocalcin (OCN) and osteopontin (OPN) within newly formed bone. Manufactured personalized construct acts as a "smart" responsive biomaterial capable of modulating the functionality and potential for the personalized bone reconstruction on a clinically relevant length scale.", publisher = "De Gruyter", journal = "Biomedizinische Technik", title = "Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction", doi = "10.1515/bmt-2019-0218" }
Micić, M., Antonijević, D., Milutinović-Smiljanić, S., Trisić, D., Colović, B., Kosanović, D., Prokić, B., Vasić, J., Živković, S., Milasin, J., Danilović, V., Đurić, M.,& Jokanović, V.. (2020). Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction. in Biomedizinische Technik De Gruyter.. https://doi.org/10.1515/bmt-2019-0218
Micić M, Antonijević D, Milutinović-Smiljanić S, Trisić D, Colović B, Kosanović D, Prokić B, Vasić J, Živković S, Milasin J, Danilović V, Đurić M, Jokanović V. Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction. in Biomedizinische Technik. 2020;. doi:10.1515/bmt-2019-0218 .
Micić, M., Antonijević, D., Milutinović-Smiljanić, Sanja, Trisić, D., Colović, B., Kosanović, D., Prokić, B., Vasić, Jugoslav, Živković, Slavoljub, Milasin, J., Danilović, V., Đurić, M., Jokanović, Vukoman, "Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction" in Biomedizinische Technik (2020), https://doi.org/10.1515/bmt-2019-0218 . .