The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions
Efekat dodavanja antioksidanasa u hrani na određene parametre kvaliteta semena bikova u uslovima toplotnog stresa
2020
Аутори
Petrović, SlobodanMaletić, Milan
Lakić, Nada
Aleksić, Nevenka
Maletić, Jelena
Ristanić, Marko
Stanimirović, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of the current research was to assess the effects of the feed additive made
of lyophilised melon juice (source of superoxide dismutase, SOD) and inactivated live
Saccharomyces cerevisiae (strain R397) cells added to the feed via the product containing
high levels of organically bound selenium (source of selenium-dependant glutathione
peroxidase, Se-GPx) on the semen quality of bulls in heat stress conditions. The 15
bulls chosen for the experiment were assigned to three equal groups (control –group
C; treated group M, given the source of SOD; and group A, treated with the source
of Se-GPx). The research was conducted in summer. The activities of SOD and SeGPx in seminal plasma were determined spectrophotometrically. Computer-assisted
semen analysis was done to determine the sperm counts, motility and velocity. The
temperature and humidity were recorded with a digital data logger.
The average SOD activity in the control bulls was significantly lower than in M
(p<0.001...) and A (p<0.001), whilst the average activities in the treated groups did not
differ significantly (p=0.784). Higher average SOD activity compared to the control in
the treated groups showed that both feed additives increased the antioxidative capacity
of the seminal fluid. The average GPx activity in the control was significantly lower
than in groups A (p=0.001) and M (p=0.005), whilst the two treatments did not lead to
significantly different results (p=0.701). The analysis of relations between the activity of
each enzyme and sperm motility and progressive motility in each of the bulls failed to
detect a significant correlation. The analysis of the relation between THI (temperaturehumidity index) and the activity of the antioxidative enzymes revealed that the increase
in THI coincided with the decrease in the SOD activity in the control group, but with
its increase in the treated groups (p>0.05). In all of the three groups with the increase
in THI there was an increase in GPx activity (p>0.05). It can be concluded that in all
of the three groups of bulls there was an increase in the activity of both enzymes in the seminal plasma, but the increase was significantly lower in the control. Thus, the
antioxidative capacity of the seminal plasma of untreated bulls was proven to be lower
in comparison with those of the treated animals.
Cilj ovog istraživanja bio je ispitivanje efekta liofi lizovanog preparata voćne pulpe dinje (izvor superoksid dismutase – SOD) i inaktivisanih živih ćelija kvasca Sacharomycess cere-visae (soj R397) dodatih u hranu kroz preparat koji sadrži visok nivo organski vezanog selena (izvor selen zavisne glutation-peroksidaze – Se-GPx) na kvalitet semena bikova u uslovima toplotnog stresa. U ogledje bilo uključeno 15 bikova podeljenih u tri grupe (kontrolna – C grupa, ogledna grupa-M kojoj je dodavan izvor SOD – i ogledna grupa – A tretirana izvorom Se-GPx). Ogled je sproveden u letnjem periodu. Aktivnosti SOD i Se-GPx u seminalnoj plazmi određivane su spektofotometrijski. Za utvrđivanje koncentracija spermatozoida, pokretljivosti i brzinskih parametara korišćena je CASA (computer-assisted semen analysis), a za merenje temeperature i vlažnosti koristili smo digi-talni data loger.Prosečna aktivnost SOD u kontrolnoj grupi bikova bila je značajno niža od one u M grupi (p<0,001) i A gr...upi (p<0,001), dok se prosečne vrednosti u tretiranim grupama nisu međusobno značajno razlikovale (p=0,784). Više prosečne vrednosti SOD su u tretiranim grupama u odnosu na kontrolnu ukazuju da su oba dodatka hrani povećala antioksidativni kapacitet semene plazme. Prosečna aktivnost GPx za kontrolnu grupu je bila značajno niža nego u A (p=0,001) i M grupi bikova (p=0.005), dok se među-sobno M i A grupa nisu značajno razlikovale (p=0,701). Analizom korelacije između aktivnosti svakog od enzima i procenta pokretljivih i progresivno pokretljivih sperma-tozoida kod svakog bika ponaosob nije ustanovljena značajna povezanost. Analizom veze između THI (temperature-humidity index) i aktivnosti antioksidativnih enzima uočeno je da je sa porastom THI u kontrolnoj grupi došlo do smanjenja aktivnosti SOD, dok je u tretiranim grupama nastalo povećanje aktivnosti SOD (p>0.05). U sve tri grupe bikova povećanjem THI povećavala se i aktivnost GPx (p>0.05). Može se zaključiti da je kod sve tri grupe bikova došlo do povećanja aktivnosti oba enzima u semenoj plazmi, ali je povećanje značajno niže u kontrolnoj grupi nego u tretiranim grupama. Time je dokazan manji antioksidativni kapacitet semene plazme netretiranih bikova u odnosu na tretirane.
Кључне речи:
antioxidative enzymes / bull / motility / spermИзвор:
Acta Veterinaria-Beograd, 2020, 70, 4, 453-470Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Финансирање / пројекти:
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200143 (Универзитет у Београду, Факултет ветеринарске медицине) (RS-MESTD-inst-2020-200143)
DOI: 10.2478/acve-2020-0034
ISSN: 0567-8315
WoS: 000606333900004
Scopus: 2-s2.0-85099337503
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Petrović, Slobodan AU - Maletić, Milan AU - Lakić, Nada AU - Aleksić, Nevenka AU - Maletić, Jelena AU - Ristanić, Marko AU - Stanimirović, Zoran PY - 2020 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2036 AB - The aim of the current research was to assess the effects of the feed additive made of lyophilised melon juice (source of superoxide dismutase, SOD) and inactivated live Saccharomyces cerevisiae (strain R397) cells added to the feed via the product containing high levels of organically bound selenium (source of selenium-dependant glutathione peroxidase, Se-GPx) on the semen quality of bulls in heat stress conditions. The 15 bulls chosen for the experiment were assigned to three equal groups (control –group C; treated group M, given the source of SOD; and group A, treated with the source of Se-GPx). The research was conducted in summer. The activities of SOD and SeGPx in seminal plasma were determined spectrophotometrically. Computer-assisted semen analysis was done to determine the sperm counts, motility and velocity. The temperature and humidity were recorded with a digital data logger. The average SOD activity in the control bulls was significantly lower than in M (p<0.001) and A (p<0.001), whilst the average activities in the treated groups did not differ significantly (p=0.784). Higher average SOD activity compared to the control in the treated groups showed that both feed additives increased the antioxidative capacity of the seminal fluid. The average GPx activity in the control was significantly lower than in groups A (p=0.001) and M (p=0.005), whilst the two treatments did not lead to significantly different results (p=0.701). The analysis of relations between the activity of each enzyme and sperm motility and progressive motility in each of the bulls failed to detect a significant correlation. The analysis of the relation between THI (temperaturehumidity index) and the activity of the antioxidative enzymes revealed that the increase in THI coincided with the decrease in the SOD activity in the control group, but with its increase in the treated groups (p>0.05). In all of the three groups with the increase in THI there was an increase in GPx activity (p>0.05). It can be concluded that in all of the three groups of bulls there was an increase in the activity of both enzymes in the seminal plasma, but the increase was significantly lower in the control. Thus, the antioxidative capacity of the seminal plasma of untreated bulls was proven to be lower in comparison with those of the treated animals. AB - Cilj ovog istraživanja bio je ispitivanje efekta liofi lizovanog preparata voćne pulpe dinje (izvor superoksid dismutase – SOD) i inaktivisanih živih ćelija kvasca Sacharomycess cere-visae (soj R397) dodatih u hranu kroz preparat koji sadrži visok nivo organski vezanog selena (izvor selen zavisne glutation-peroksidaze – Se-GPx) na kvalitet semena bikova u uslovima toplotnog stresa. U ogledje bilo uključeno 15 bikova podeljenih u tri grupe (kontrolna – C grupa, ogledna grupa-M kojoj je dodavan izvor SOD – i ogledna grupa – A tretirana izvorom Se-GPx). Ogled je sproveden u letnjem periodu. Aktivnosti SOD i Se-GPx u seminalnoj plazmi određivane su spektofotometrijski. Za utvrđivanje koncentracija spermatozoida, pokretljivosti i brzinskih parametara korišćena je CASA (computer-assisted semen analysis), a za merenje temeperature i vlažnosti koristili smo digi-talni data loger.Prosečna aktivnost SOD u kontrolnoj grupi bikova bila je značajno niža od one u M grupi (p<0,001) i A grupi (p<0,001), dok se prosečne vrednosti u tretiranim grupama nisu međusobno značajno razlikovale (p=0,784). Više prosečne vrednosti SOD su u tretiranim grupama u odnosu na kontrolnu ukazuju da su oba dodatka hrani povećala antioksidativni kapacitet semene plazme. Prosečna aktivnost GPx za kontrolnu grupu je bila značajno niža nego u A (p=0,001) i M grupi bikova (p=0.005), dok se među-sobno M i A grupa nisu značajno razlikovale (p=0,701). Analizom korelacije između aktivnosti svakog od enzima i procenta pokretljivih i progresivno pokretljivih sperma-tozoida kod svakog bika ponaosob nije ustanovljena značajna povezanost. Analizom veze između THI (temperature-humidity index) i aktivnosti antioksidativnih enzima uočeno je da je sa porastom THI u kontrolnoj grupi došlo do smanjenja aktivnosti SOD, dok je u tretiranim grupama nastalo povećanje aktivnosti SOD (p>0.05). U sve tri grupe bikova povećanjem THI povećavala se i aktivnost GPx (p>0.05). Može se zaključiti da je kod sve tri grupe bikova došlo do povećanja aktivnosti oba enzima u semenoj plazmi, ali je povećanje značajno niže u kontrolnoj grupi nego u tretiranim grupama. Time je dokazan manji antioksidativni kapacitet semene plazme netretiranih bikova u odnosu na tretirane. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions T1 - Efekat dodavanja antioksidanasa u hrani na određene parametre kvaliteta semena bikova u uslovima toplotnog stresa VL - 70 IS - 4 SP - 453 EP - 470 DO - 10.2478/acve-2020-0034 ER -
@article{ author = "Petrović, Slobodan and Maletić, Milan and Lakić, Nada and Aleksić, Nevenka and Maletić, Jelena and Ristanić, Marko and Stanimirović, Zoran", year = "2020", abstract = "The aim of the current research was to assess the effects of the feed additive made of lyophilised melon juice (source of superoxide dismutase, SOD) and inactivated live Saccharomyces cerevisiae (strain R397) cells added to the feed via the product containing high levels of organically bound selenium (source of selenium-dependant glutathione peroxidase, Se-GPx) on the semen quality of bulls in heat stress conditions. The 15 bulls chosen for the experiment were assigned to three equal groups (control –group C; treated group M, given the source of SOD; and group A, treated with the source of Se-GPx). The research was conducted in summer. The activities of SOD and SeGPx in seminal plasma were determined spectrophotometrically. Computer-assisted semen analysis was done to determine the sperm counts, motility and velocity. The temperature and humidity were recorded with a digital data logger. The average SOD activity in the control bulls was significantly lower than in M (p<0.001) and A (p<0.001), whilst the average activities in the treated groups did not differ significantly (p=0.784). Higher average SOD activity compared to the control in the treated groups showed that both feed additives increased the antioxidative capacity of the seminal fluid. The average GPx activity in the control was significantly lower than in groups A (p=0.001) and M (p=0.005), whilst the two treatments did not lead to significantly different results (p=0.701). The analysis of relations between the activity of each enzyme and sperm motility and progressive motility in each of the bulls failed to detect a significant correlation. The analysis of the relation between THI (temperaturehumidity index) and the activity of the antioxidative enzymes revealed that the increase in THI coincided with the decrease in the SOD activity in the control group, but with its increase in the treated groups (p>0.05). In all of the three groups with the increase in THI there was an increase in GPx activity (p>0.05). It can be concluded that in all of the three groups of bulls there was an increase in the activity of both enzymes in the seminal plasma, but the increase was significantly lower in the control. Thus, the antioxidative capacity of the seminal plasma of untreated bulls was proven to be lower in comparison with those of the treated animals., Cilj ovog istraživanja bio je ispitivanje efekta liofi lizovanog preparata voćne pulpe dinje (izvor superoksid dismutase – SOD) i inaktivisanih živih ćelija kvasca Sacharomycess cere-visae (soj R397) dodatih u hranu kroz preparat koji sadrži visok nivo organski vezanog selena (izvor selen zavisne glutation-peroksidaze – Se-GPx) na kvalitet semena bikova u uslovima toplotnog stresa. U ogledje bilo uključeno 15 bikova podeljenih u tri grupe (kontrolna – C grupa, ogledna grupa-M kojoj je dodavan izvor SOD – i ogledna grupa – A tretirana izvorom Se-GPx). Ogled je sproveden u letnjem periodu. Aktivnosti SOD i Se-GPx u seminalnoj plazmi određivane su spektofotometrijski. Za utvrđivanje koncentracija spermatozoida, pokretljivosti i brzinskih parametara korišćena je CASA (computer-assisted semen analysis), a za merenje temeperature i vlažnosti koristili smo digi-talni data loger.Prosečna aktivnost SOD u kontrolnoj grupi bikova bila je značajno niža od one u M grupi (p<0,001) i A grupi (p<0,001), dok se prosečne vrednosti u tretiranim grupama nisu međusobno značajno razlikovale (p=0,784). Više prosečne vrednosti SOD su u tretiranim grupama u odnosu na kontrolnu ukazuju da su oba dodatka hrani povećala antioksidativni kapacitet semene plazme. Prosečna aktivnost GPx za kontrolnu grupu je bila značajno niža nego u A (p=0,001) i M grupi bikova (p=0.005), dok se među-sobno M i A grupa nisu značajno razlikovale (p=0,701). Analizom korelacije između aktivnosti svakog od enzima i procenta pokretljivih i progresivno pokretljivih sperma-tozoida kod svakog bika ponaosob nije ustanovljena značajna povezanost. Analizom veze između THI (temperature-humidity index) i aktivnosti antioksidativnih enzima uočeno je da je sa porastom THI u kontrolnoj grupi došlo do smanjenja aktivnosti SOD, dok je u tretiranim grupama nastalo povećanje aktivnosti SOD (p>0.05). U sve tri grupe bikova povećanjem THI povećavala se i aktivnost GPx (p>0.05). Može se zaključiti da je kod sve tri grupe bikova došlo do povećanja aktivnosti oba enzima u semenoj plazmi, ali je povećanje značajno niže u kontrolnoj grupi nego u tretiranim grupama. Time je dokazan manji antioksidativni kapacitet semene plazme netretiranih bikova u odnosu na tretirane.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions, Efekat dodavanja antioksidanasa u hrani na određene parametre kvaliteta semena bikova u uslovima toplotnog stresa", volume = "70", number = "4", pages = "453-470", doi = "10.2478/acve-2020-0034" }
Petrović, S., Maletić, M., Lakić, N., Aleksić, N., Maletić, J., Ristanić, M.,& Stanimirović, Z.. (2020). The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(4), 453-470. https://doi.org/10.2478/acve-2020-0034
Petrović S, Maletić M, Lakić N, Aleksić N, Maletić J, Ristanić M, Stanimirović Z. The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions. in Acta Veterinaria-Beograd. 2020;70(4):453-470. doi:10.2478/acve-2020-0034 .
Petrović, Slobodan, Maletić, Milan, Lakić, Nada, Aleksić, Nevenka, Maletić, Jelena, Ristanić, Marko, Stanimirović, Zoran, "The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions" in Acta Veterinaria-Beograd, 70, no. 4 (2020):453-470, https://doi.org/10.2478/acve-2020-0034 . .