Antisperm antibodies of the Ig a class in the cervical mucus and sera of artificially inseminated cows
Ig A antititela protiv spermatozoida u cervikalnoj sluzi i serumu krava koje se veštački osemenjavaju
Abstract
The aim of this study was to investigate the level of anti-sperm antibodies of the Ig A class in the cervical mucus and sera of artificially inseminated cows. Cervical mucus and sera samples were collected on the day of artificial insemination and the animals were divided into four groups of 20 cows each according to the number of previous inseminations. The titer of antibodies was determined by the indirect immunofluorescence method using sperm cells suspended in Tris egg-yolk extender or the commercial extender "Biociphos +". Our results indicate that titers of antisperm antibodies of the Ig A class elevate with the number of artificial inseminations. Mean titer values were higher in the cervical mucus samples than in sera indicating that the local immune response is more relevant for the immunological reactivity to sperm and extender antigens. In addition, the titer of antisperm antibodies was generally higher when sperm cells suspended in Tris egg-yolk extender were used for the te...st.
Cilj ovih ispitivanja je bio da se utvrdi nivo antispermatozoalnih antitela Ig A klase u krvnom serumu i cervikalnoj sluzi krava koje se veštački osemenjavaju. Uzorci krvi i cervikalne sluzi su prikupljani na dan veštačkog osemenjavanja a životinje su bile podeljebne u četiri grupe na osnovu broja prethodnih osemenjavanja. Prisustvo antitspermatozoalnih antitela je određivano metodom indirektne imunofluorescence korišćenjem spermatozoida suspendovanih u Tris-žumanjčanom razređivaču i komercijalnom razređivaču "Biociphos +". Postignuti rezulati ukazuju da titar antispermatozoalnih Ig A antitela raste sa brojem osemenjavanja i ima veće vrednosti u cervikalnoj sluzi nego u serumu. Ovi rezultati potvrđuju hipotezu o značaju lokalnog imunskog odgovora na antigene spermatozoida i razređivača za spermu bika. Osim toga titar antitela je imao veće vrednosti kada su za izvođenje testa korišćeni spermatozoidi suspendovani u Tris-žumanjčanom razređivaču.
Keywords:
cows / subfertility / Ig A / cervical mucus / sera / sperm cellsSource:
Acta Veterinaria-Beograd, 2003, 53, 5-6, 311-320Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
DOI: 10.2298/AVB0306311L
ISSN: 0567-8315
WoS: 000187620300003
Scopus: 2-s2.0-1642493622
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Fakultet veterinarske medicineTY - JOUR AU - Lazarević, Miodrag AU - Milanović, Svetlana AU - Kirovski, Danijela AU - Milovanović, Aleksandar PY - 2003 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/245 AB - The aim of this study was to investigate the level of anti-sperm antibodies of the Ig A class in the cervical mucus and sera of artificially inseminated cows. Cervical mucus and sera samples were collected on the day of artificial insemination and the animals were divided into four groups of 20 cows each according to the number of previous inseminations. The titer of antibodies was determined by the indirect immunofluorescence method using sperm cells suspended in Tris egg-yolk extender or the commercial extender "Biociphos +". Our results indicate that titers of antisperm antibodies of the Ig A class elevate with the number of artificial inseminations. Mean titer values were higher in the cervical mucus samples than in sera indicating that the local immune response is more relevant for the immunological reactivity to sperm and extender antigens. In addition, the titer of antisperm antibodies was generally higher when sperm cells suspended in Tris egg-yolk extender were used for the test. AB - Cilj ovih ispitivanja je bio da se utvrdi nivo antispermatozoalnih antitela Ig A klase u krvnom serumu i cervikalnoj sluzi krava koje se veštački osemenjavaju. Uzorci krvi i cervikalne sluzi su prikupljani na dan veštačkog osemenjavanja a životinje su bile podeljebne u četiri grupe na osnovu broja prethodnih osemenjavanja. Prisustvo antitspermatozoalnih antitela je određivano metodom indirektne imunofluorescence korišćenjem spermatozoida suspendovanih u Tris-žumanjčanom razređivaču i komercijalnom razređivaču "Biociphos +". Postignuti rezulati ukazuju da titar antispermatozoalnih Ig A antitela raste sa brojem osemenjavanja i ima veće vrednosti u cervikalnoj sluzi nego u serumu. Ovi rezultati potvrđuju hipotezu o značaju lokalnog imunskog odgovora na antigene spermatozoida i razređivača za spermu bika. Osim toga titar antitela je imao veće vrednosti kada su za izvođenje testa korišćeni spermatozoidi suspendovani u Tris-žumanjčanom razređivaču. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Antisperm antibodies of the Ig a class in the cervical mucus and sera of artificially inseminated cows T1 - Ig A antititela protiv spermatozoida u cervikalnoj sluzi i serumu krava koje se veštački osemenjavaju VL - 53 IS - 5-6 SP - 311 EP - 320 DO - 10.2298/AVB0306311L ER -
@article{ author = "Lazarević, Miodrag and Milanović, Svetlana and Kirovski, Danijela and Milovanović, Aleksandar", year = "2003", abstract = "The aim of this study was to investigate the level of anti-sperm antibodies of the Ig A class in the cervical mucus and sera of artificially inseminated cows. Cervical mucus and sera samples were collected on the day of artificial insemination and the animals were divided into four groups of 20 cows each according to the number of previous inseminations. The titer of antibodies was determined by the indirect immunofluorescence method using sperm cells suspended in Tris egg-yolk extender or the commercial extender "Biociphos +". Our results indicate that titers of antisperm antibodies of the Ig A class elevate with the number of artificial inseminations. Mean titer values were higher in the cervical mucus samples than in sera indicating that the local immune response is more relevant for the immunological reactivity to sperm and extender antigens. In addition, the titer of antisperm antibodies was generally higher when sperm cells suspended in Tris egg-yolk extender were used for the test., Cilj ovih ispitivanja je bio da se utvrdi nivo antispermatozoalnih antitela Ig A klase u krvnom serumu i cervikalnoj sluzi krava koje se veštački osemenjavaju. Uzorci krvi i cervikalne sluzi su prikupljani na dan veštačkog osemenjavanja a životinje su bile podeljebne u četiri grupe na osnovu broja prethodnih osemenjavanja. Prisustvo antitspermatozoalnih antitela je određivano metodom indirektne imunofluorescence korišćenjem spermatozoida suspendovanih u Tris-žumanjčanom razređivaču i komercijalnom razređivaču "Biociphos +". Postignuti rezulati ukazuju da titar antispermatozoalnih Ig A antitela raste sa brojem osemenjavanja i ima veće vrednosti u cervikalnoj sluzi nego u serumu. Ovi rezultati potvrđuju hipotezu o značaju lokalnog imunskog odgovora na antigene spermatozoida i razređivača za spermu bika. Osim toga titar antitela je imao veće vrednosti kada su za izvođenje testa korišćeni spermatozoidi suspendovani u Tris-žumanjčanom razređivaču.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Antisperm antibodies of the Ig a class in the cervical mucus and sera of artificially inseminated cows, Ig A antititela protiv spermatozoida u cervikalnoj sluzi i serumu krava koje se veštački osemenjavaju", volume = "53", number = "5-6", pages = "311-320", doi = "10.2298/AVB0306311L" }
Lazarević, M., Milanović, S., Kirovski, D.,& Milovanović, A.. (2003). Antisperm antibodies of the Ig a class in the cervical mucus and sera of artificially inseminated cows. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 53(5-6), 311-320. https://doi.org/10.2298/AVB0306311L
Lazarević M, Milanović S, Kirovski D, Milovanović A. Antisperm antibodies of the Ig a class in the cervical mucus and sera of artificially inseminated cows. in Acta Veterinaria-Beograd. 2003;53(5-6):311-320. doi:10.2298/AVB0306311L .
Lazarević, Miodrag, Milanović, Svetlana, Kirovski, Danijela, Milovanović, Aleksandar, "Antisperm antibodies of the Ig a class in the cervical mucus and sera of artificially inseminated cows" in Acta Veterinaria-Beograd, 53, no. 5-6 (2003):311-320, https://doi.org/10.2298/AVB0306311L . .