Evaluation of genotoxic and genoprotective effects of Agaricus bisporus extract on AmE-711 honey bee cell line in the Comet assay
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2022
Authors
Rajković, MilanStanimirović, Zoran
Stevanović, Jevrosima
Ristanić, Marko
Vejnović, Branislav
Goblirsch, Michael
Glavinić, Uroš
Article (Published version)
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Mushroom extracts have been shown to contain bioactive compounds that confer health benefits to humans and animals, including honey bees. Honey bees are often challenged simultaneously with several abiotic (e.g., pesticides and poor nutrition) and biotic (e.g., parasites and pathogens) stressors. Mushroom extracts, for example, those made from Agaricus bisporus, may hold the potential to mitigate the negative effects of these stressors through actions on the honey bee’s immune system, metabolism, and other physiological processes. Exploring the health benefits of mushroom extracts for honey bees requires a basic understanding of their bioactive properties at the cellular level. The aim of the present study was to evaluate, for the first time, the genotoxic and antigenotoxic potential of A. bisporus on the honey bee cell line AmE-711 using the comet assay. Three increasing concentrations of A. bisporus water extract (100, 200, and 400 µg/mL) were tested. The cells in the negative contro...l group were not treated either with A. bisporus extract or with H2O2. In the positive control group, DNA damage was induced with 100 µM H2O2. For antigenotoxic effect, tested extracts were mixed and incubated with H2O2. None of the tested concentrations exerted genotoxic potential, but all showed antigenotoxic effects against H2O2-induced DNA damage. The concentration of 200 μg/mL A. bisporus extract was the most effective in its action against DNA damage. To conclude, A. bisporus extract did not demonstrate genotoxic effects but showed promising antigenotoxic properties. AmE-711 cell line may serve as a cell culture system for genotoxicity investigations.
Keywords:
antigenotoxic effect / Apis mellifera / continuous cell line / single cell gel electrophoresis / white button mushroomSource:
Journal of Apicultural Research, 2022Publisher:
- Taylor & Francis
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200143 (University of Belgrade, Faculty of Veterinary Medicine) (RS-MESTD-inst-2020-200143)
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Fakultet veterinarske medicineTY - JOUR AU - Rajković, Milan AU - Stanimirović, Zoran AU - Stevanović, Jevrosima AU - Ristanić, Marko AU - Vejnović, Branislav AU - Goblirsch, Michael AU - Glavinić, Uroš PY - 2022 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2502 AB - Mushroom extracts have been shown to contain bioactive compounds that confer health benefits to humans and animals, including honey bees. Honey bees are often challenged simultaneously with several abiotic (e.g., pesticides and poor nutrition) and biotic (e.g., parasites and pathogens) stressors. Mushroom extracts, for example, those made from Agaricus bisporus, may hold the potential to mitigate the negative effects of these stressors through actions on the honey bee’s immune system, metabolism, and other physiological processes. Exploring the health benefits of mushroom extracts for honey bees requires a basic understanding of their bioactive properties at the cellular level. The aim of the present study was to evaluate, for the first time, the genotoxic and antigenotoxic potential of A. bisporus on the honey bee cell line AmE-711 using the comet assay. Three increasing concentrations of A. bisporus water extract (100, 200, and 400 µg/mL) were tested. The cells in the negative control group were not treated either with A. bisporus extract or with H2O2. In the positive control group, DNA damage was induced with 100 µM H2O2. For antigenotoxic effect, tested extracts were mixed and incubated with H2O2. None of the tested concentrations exerted genotoxic potential, but all showed antigenotoxic effects against H2O2-induced DNA damage. The concentration of 200 μg/mL A. bisporus extract was the most effective in its action against DNA damage. To conclude, A. bisporus extract did not demonstrate genotoxic effects but showed promising antigenotoxic properties. AmE-711 cell line may serve as a cell culture system for genotoxicity investigations. PB - Taylor & Francis T2 - Journal of Apicultural Research T2 - Journal of Apicultural Research T1 - Evaluation of genotoxic and genoprotective effects of Agaricus bisporus extract on AmE-711 honey bee cell line in the Comet assay DO - 10.1080/00218839.2022.2146367 ER -
@article{ author = "Rajković, Milan and Stanimirović, Zoran and Stevanović, Jevrosima and Ristanić, Marko and Vejnović, Branislav and Goblirsch, Michael and Glavinić, Uroš", year = "2022", abstract = "Mushroom extracts have been shown to contain bioactive compounds that confer health benefits to humans and animals, including honey bees. Honey bees are often challenged simultaneously with several abiotic (e.g., pesticides and poor nutrition) and biotic (e.g., parasites and pathogens) stressors. Mushroom extracts, for example, those made from Agaricus bisporus, may hold the potential to mitigate the negative effects of these stressors through actions on the honey bee’s immune system, metabolism, and other physiological processes. Exploring the health benefits of mushroom extracts for honey bees requires a basic understanding of their bioactive properties at the cellular level. The aim of the present study was to evaluate, for the first time, the genotoxic and antigenotoxic potential of A. bisporus on the honey bee cell line AmE-711 using the comet assay. Three increasing concentrations of A. bisporus water extract (100, 200, and 400 µg/mL) were tested. The cells in the negative control group were not treated either with A. bisporus extract or with H2O2. In the positive control group, DNA damage was induced with 100 µM H2O2. For antigenotoxic effect, tested extracts were mixed and incubated with H2O2. None of the tested concentrations exerted genotoxic potential, but all showed antigenotoxic effects against H2O2-induced DNA damage. The concentration of 200 μg/mL A. bisporus extract was the most effective in its action against DNA damage. To conclude, A. bisporus extract did not demonstrate genotoxic effects but showed promising antigenotoxic properties. AmE-711 cell line may serve as a cell culture system for genotoxicity investigations.", publisher = "Taylor & Francis", journal = "Journal of Apicultural Research, Journal of Apicultural Research", title = "Evaluation of genotoxic and genoprotective effects of Agaricus bisporus extract on AmE-711 honey bee cell line in the Comet assay", doi = "10.1080/00218839.2022.2146367" }
Rajković, M., Stanimirović, Z., Stevanović, J., Ristanić, M., Vejnović, B., Goblirsch, M.,& Glavinić, U.. (2022). Evaluation of genotoxic and genoprotective effects of Agaricus bisporus extract on AmE-711 honey bee cell line in the Comet assay. in Journal of Apicultural Research Taylor & Francis.. https://doi.org/10.1080/00218839.2022.2146367
Rajković M, Stanimirović Z, Stevanović J, Ristanić M, Vejnović B, Goblirsch M, Glavinić U. Evaluation of genotoxic and genoprotective effects of Agaricus bisporus extract on AmE-711 honey bee cell line in the Comet assay. in Journal of Apicultural Research. 2022;. doi:10.1080/00218839.2022.2146367 .
Rajković, Milan, Stanimirović, Zoran, Stevanović, Jevrosima, Ristanić, Marko, Vejnović, Branislav, Goblirsch, Michael, Glavinić, Uroš, "Evaluation of genotoxic and genoprotective effects of Agaricus bisporus extract on AmE-711 honey bee cell line in the Comet assay" in Journal of Apicultural Research (2022), https://doi.org/10.1080/00218839.2022.2146367 . .