Analysis of sister-chromatid exchanges in cultured human lymphocytes treated with cymiazole hydrochloride
Analiza razmene sestrinskih hromatida u kulturama limfocita čoveka tretiranih cimiazol hidrohloridom
Апстракт
Cymiazole hydrochloride is the active component of the acaricides Apitol® and Apichem®, which are used as varroacides in beekeeping. Considering that residues of cymiazole hydrochloride were detected in all bee products being used for human nutrition and as alternative medications in human and veterinary medicine, the aim of this study was to evaluate the ability of this substance to produce genotoxic effects in human peripheral blood lymphocytes. The genotoxic effects were studied by sister chromatid exchange (SCE) test in vitro. The results obtained for all doses applied to the cultures (0.01; 0.10 and 1 mg/ml) showed very significant increases in frequencies (p lt 0.001) of SCEs in comparison to the negative control, i.e. cymiazole hydrochloride exhibited genotoxic properties.
Cimiazol hidrohlorid je aktivna komponenta Apitol®-a i Apichem®-a koji se koriste kao varoacidi u gajenju pčela. Kako su rezidue cimiazol hidrohlorida utvrđene u svim pčelinjim proizvodima - kako onima koji se koriste u ljudskoj ishrani, tako i onima koji se koriste kao alternativni medikamenti u ljudskoj i veterinarskoj medicini, cilj ovog proučavanja je bio da se procene mogućnosti ove substance da izazove genotoksična dejstva na limfocite periferne krvi čoveka. Efekti genotoksičnosti ispitivani su u in vitro SCE testu. Dobijeni rezultati, za sve doze koje su primenjene u kulturama (0.01; 0.10 and 1 mg/ml), pokazali su statistički vrlo značajno povećanje (p lt 0.001) učestalosti SCEa u poređenju sa negativnom kontrolom tj. cimiazol hidrohlorid je, pri korišćenim uslovima eksperimenta, iskazao genotoksična svojstva u kulturi humanih limfocita.
Кључне речи:
cymiazole hydrochloride / acaricides / varroacides / pesticides / genotoxicity / SCE-test / chromosomal aberrations / beekeepingИзвор:
Acta Veterinaria-Beograd, 2003, 53, 5-6, 419-426Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
DOI: 10.2298/AVB0306419S
ISSN: 0567-8315
WoS: 000187620300015
Scopus: 2-s2.0-1642534319
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Stanimirović, Zoran AU - Fišter, Svetlana L. AU - Stevanović, Jevrosima PY - 2003 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/257 AB - Cymiazole hydrochloride is the active component of the acaricides Apitol® and Apichem®, which are used as varroacides in beekeeping. Considering that residues of cymiazole hydrochloride were detected in all bee products being used for human nutrition and as alternative medications in human and veterinary medicine, the aim of this study was to evaluate the ability of this substance to produce genotoxic effects in human peripheral blood lymphocytes. The genotoxic effects were studied by sister chromatid exchange (SCE) test in vitro. The results obtained for all doses applied to the cultures (0.01; 0.10 and 1 mg/ml) showed very significant increases in frequencies (p lt 0.001) of SCEs in comparison to the negative control, i.e. cymiazole hydrochloride exhibited genotoxic properties. AB - Cimiazol hidrohlorid je aktivna komponenta Apitol®-a i Apichem®-a koji se koriste kao varoacidi u gajenju pčela. Kako su rezidue cimiazol hidrohlorida utvrđene u svim pčelinjim proizvodima - kako onima koji se koriste u ljudskoj ishrani, tako i onima koji se koriste kao alternativni medikamenti u ljudskoj i veterinarskoj medicini, cilj ovog proučavanja je bio da se procene mogućnosti ove substance da izazove genotoksična dejstva na limfocite periferne krvi čoveka. Efekti genotoksičnosti ispitivani su u in vitro SCE testu. Dobijeni rezultati, za sve doze koje su primenjene u kulturama (0.01; 0.10 and 1 mg/ml), pokazali su statistički vrlo značajno povećanje (p lt 0.001) učestalosti SCEa u poređenju sa negativnom kontrolom tj. cimiazol hidrohlorid je, pri korišćenim uslovima eksperimenta, iskazao genotoksična svojstva u kulturi humanih limfocita. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Analysis of sister-chromatid exchanges in cultured human lymphocytes treated with cymiazole hydrochloride T1 - Analiza razmene sestrinskih hromatida u kulturama limfocita čoveka tretiranih cimiazol hidrohloridom VL - 53 IS - 5-6 SP - 419 EP - 426 DO - 10.2298/AVB0306419S ER -
@article{ author = "Stanimirović, Zoran and Fišter, Svetlana L. and Stevanović, Jevrosima", year = "2003", abstract = "Cymiazole hydrochloride is the active component of the acaricides Apitol® and Apichem®, which are used as varroacides in beekeeping. Considering that residues of cymiazole hydrochloride were detected in all bee products being used for human nutrition and as alternative medications in human and veterinary medicine, the aim of this study was to evaluate the ability of this substance to produce genotoxic effects in human peripheral blood lymphocytes. The genotoxic effects were studied by sister chromatid exchange (SCE) test in vitro. The results obtained for all doses applied to the cultures (0.01; 0.10 and 1 mg/ml) showed very significant increases in frequencies (p lt 0.001) of SCEs in comparison to the negative control, i.e. cymiazole hydrochloride exhibited genotoxic properties., Cimiazol hidrohlorid je aktivna komponenta Apitol®-a i Apichem®-a koji se koriste kao varoacidi u gajenju pčela. Kako su rezidue cimiazol hidrohlorida utvrđene u svim pčelinjim proizvodima - kako onima koji se koriste u ljudskoj ishrani, tako i onima koji se koriste kao alternativni medikamenti u ljudskoj i veterinarskoj medicini, cilj ovog proučavanja je bio da se procene mogućnosti ove substance da izazove genotoksična dejstva na limfocite periferne krvi čoveka. Efekti genotoksičnosti ispitivani su u in vitro SCE testu. Dobijeni rezultati, za sve doze koje su primenjene u kulturama (0.01; 0.10 and 1 mg/ml), pokazali su statistički vrlo značajno povećanje (p lt 0.001) učestalosti SCEa u poređenju sa negativnom kontrolom tj. cimiazol hidrohlorid je, pri korišćenim uslovima eksperimenta, iskazao genotoksična svojstva u kulturi humanih limfocita.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Analysis of sister-chromatid exchanges in cultured human lymphocytes treated with cymiazole hydrochloride, Analiza razmene sestrinskih hromatida u kulturama limfocita čoveka tretiranih cimiazol hidrohloridom", volume = "53", number = "5-6", pages = "419-426", doi = "10.2298/AVB0306419S" }
Stanimirović, Z., Fišter, S. L.,& Stevanović, J.. (2003). Analysis of sister-chromatid exchanges in cultured human lymphocytes treated with cymiazole hydrochloride. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 53(5-6), 419-426. https://doi.org/10.2298/AVB0306419S
Stanimirović Z, Fišter SL, Stevanović J. Analysis of sister-chromatid exchanges in cultured human lymphocytes treated with cymiazole hydrochloride. in Acta Veterinaria-Beograd. 2003;53(5-6):419-426. doi:10.2298/AVB0306419S .
Stanimirović, Zoran, Fišter, Svetlana L., Stevanović, Jevrosima, "Analysis of sister-chromatid exchanges in cultured human lymphocytes treated with cymiazole hydrochloride" in Acta Veterinaria-Beograd, 53, no. 5-6 (2003):419-426, https://doi.org/10.2298/AVB0306419S . .