The effect of thiamine on activity of enzymes (with a special emphasis on MAPK) in the brain of Japanese quails treated with chlorpyrifos
Аутори
Ćupić Miladinović, DejanaBorozan, Sunčica
Peković, Sanja
Dacić, Sanja
Đukić-Ćosić, Danijela
Ćupić, Vitomir
Ivanović, Saša
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to investigate the influence of vitamin B1 (thiamine) on biochemical changes in the brain tissue of Japanese quail (Coturnix japanica) treated with chlorpyrifos. The following parameters were examined: cholinesterase activity (acetylcholinesterase – AChE and butyrylcholinesterase – BChE), nitrite concentration–NO2- (parameter of oxidative/nitrosative stress), activity of inducible nitric oxide synthase –iNOS, arylesterase –ARE, cyclooxygenase –COX and extracellular signal–regulated kinase –ERK (MAPK).
The study was conducted on eighty male Japanese quails (2 controls and 6 experimental groups, n= 10), 3-4 weeks old. One control group was treated only with vitamin B1, while the second one received pure corn oil. CPF dissolved in corn oil was administered to three groups of quails by gavage for 7 consecutive days at doses of 1.5 mg/kg BW, 3 mg/kg BW and 6 mg/kg BW. Another three groups were treated with 10 mg/kg BW of vitamin B1 i.m. 30 min after CPF administr...ation (in above mentioned doses) for 7 consecutive days. Our studies have shown that CPF significantly inhibited both cholinesterases and ARE in brain, while vitamin B1 increased activity of enzymes in a dose dependent way. Also CPF has led to increase in the concentration of NO2-, activity of iNOS and COX, but after thiamine treatment there has been a decrease of these parameters. There has been a decrease of ERK expression after CPF treatment that demonstrates an increase of apoptotic vulnerability of cells exposed to CPF.
Overall these results confirm that CPF causes oxidative/nitrosative stress and apoptosis, but also support the hypothesis that thiamine belongs to the group of "antistress vitamins".
Кључне речи:
acetylcholinesterase / butyrylcholinesterase / oxidative/nitrosative stress / arylesterase / apoptosisИзвор:
10th Congress of Toxicology in Developing Countries (CTDC 10) and the 12th Serbian Congress of Toxicology, Belgrade, April 18-21, 2018, 2018, 152-153Издавач:
- Beograd : Serbian Society of Toxicology
Финансирање / пројекти:
- Унапређење и развој хигијенских и технолошких поступака у производњи намирница животињског порекла у циљу добијања квалитетних и безбедних производа конкурентних на светском тржишту (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46009)
Напомена:
- Book of Abstracts
Колекције
Институција/група
Fakultet veterinarske medicineTY - CONF AU - Ćupić Miladinović, Dejana AU - Borozan, Sunčica AU - Peković, Sanja AU - Dacić, Sanja AU - Đukić-Ćosić, Danijela AU - Ćupić, Vitomir AU - Ivanović, Saša PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2647 AB - The aim of this study was to investigate the influence of vitamin B1 (thiamine) on biochemical changes in the brain tissue of Japanese quail (Coturnix japanica) treated with chlorpyrifos. The following parameters were examined: cholinesterase activity (acetylcholinesterase – AChE and butyrylcholinesterase – BChE), nitrite concentration–NO2- (parameter of oxidative/nitrosative stress), activity of inducible nitric oxide synthase –iNOS, arylesterase –ARE, cyclooxygenase –COX and extracellular signal–regulated kinase –ERK (MAPK). The study was conducted on eighty male Japanese quails (2 controls and 6 experimental groups, n= 10), 3-4 weeks old. One control group was treated only with vitamin B1, while the second one received pure corn oil. CPF dissolved in corn oil was administered to three groups of quails by gavage for 7 consecutive days at doses of 1.5 mg/kg BW, 3 mg/kg BW and 6 mg/kg BW. Another three groups were treated with 10 mg/kg BW of vitamin B1 i.m. 30 min after CPF administration (in above mentioned doses) for 7 consecutive days. Our studies have shown that CPF significantly inhibited both cholinesterases and ARE in brain, while vitamin B1 increased activity of enzymes in a dose dependent way. Also CPF has led to increase in the concentration of NO2-, activity of iNOS and COX, but after thiamine treatment there has been a decrease of these parameters. There has been a decrease of ERK expression after CPF treatment that demonstrates an increase of apoptotic vulnerability of cells exposed to CPF. Overall these results confirm that CPF causes oxidative/nitrosative stress and apoptosis, but also support the hypothesis that thiamine belongs to the group of "antistress vitamins". PB - Beograd : Serbian Society of Toxicology C3 - 10th Congress of Toxicology in Developing Countries (CTDC 10) and the 12th Serbian Congress of Toxicology, Belgrade, April 18-21, 2018 T1 - The effect of thiamine on activity of enzymes (with a special emphasis on MAPK) in the brain of Japanese quails treated with chlorpyrifos SP - 152 EP - 153 UR - https://hdl.handle.net/21.15107/rcub_veterinar_2647 ER -
@conference{ author = "Ćupić Miladinović, Dejana and Borozan, Sunčica and Peković, Sanja and Dacić, Sanja and Đukić-Ćosić, Danijela and Ćupić, Vitomir and Ivanović, Saša", year = "2018", abstract = "The aim of this study was to investigate the influence of vitamin B1 (thiamine) on biochemical changes in the brain tissue of Japanese quail (Coturnix japanica) treated with chlorpyrifos. The following parameters were examined: cholinesterase activity (acetylcholinesterase – AChE and butyrylcholinesterase – BChE), nitrite concentration–NO2- (parameter of oxidative/nitrosative stress), activity of inducible nitric oxide synthase –iNOS, arylesterase –ARE, cyclooxygenase –COX and extracellular signal–regulated kinase –ERK (MAPK). The study was conducted on eighty male Japanese quails (2 controls and 6 experimental groups, n= 10), 3-4 weeks old. One control group was treated only with vitamin B1, while the second one received pure corn oil. CPF dissolved in corn oil was administered to three groups of quails by gavage for 7 consecutive days at doses of 1.5 mg/kg BW, 3 mg/kg BW and 6 mg/kg BW. Another three groups were treated with 10 mg/kg BW of vitamin B1 i.m. 30 min after CPF administration (in above mentioned doses) for 7 consecutive days. Our studies have shown that CPF significantly inhibited both cholinesterases and ARE in brain, while vitamin B1 increased activity of enzymes in a dose dependent way. Also CPF has led to increase in the concentration of NO2-, activity of iNOS and COX, but after thiamine treatment there has been a decrease of these parameters. There has been a decrease of ERK expression after CPF treatment that demonstrates an increase of apoptotic vulnerability of cells exposed to CPF. Overall these results confirm that CPF causes oxidative/nitrosative stress and apoptosis, but also support the hypothesis that thiamine belongs to the group of "antistress vitamins".", publisher = "Beograd : Serbian Society of Toxicology", journal = "10th Congress of Toxicology in Developing Countries (CTDC 10) and the 12th Serbian Congress of Toxicology, Belgrade, April 18-21, 2018", title = "The effect of thiamine on activity of enzymes (with a special emphasis on MAPK) in the brain of Japanese quails treated with chlorpyrifos", pages = "152-153", url = "https://hdl.handle.net/21.15107/rcub_veterinar_2647" }
Ćupić Miladinović, D., Borozan, S., Peković, S., Dacić, S., Đukić-Ćosić, D., Ćupić, V.,& Ivanović, S.. (2018). The effect of thiamine on activity of enzymes (with a special emphasis on MAPK) in the brain of Japanese quails treated with chlorpyrifos. in 10th Congress of Toxicology in Developing Countries (CTDC 10) and the 12th Serbian Congress of Toxicology, Belgrade, April 18-21, 2018 Beograd : Serbian Society of Toxicology., 152-153. https://hdl.handle.net/21.15107/rcub_veterinar_2647
Ćupić Miladinović D, Borozan S, Peković S, Dacić S, Đukić-Ćosić D, Ćupić V, Ivanović S. The effect of thiamine on activity of enzymes (with a special emphasis on MAPK) in the brain of Japanese quails treated with chlorpyrifos. in 10th Congress of Toxicology in Developing Countries (CTDC 10) and the 12th Serbian Congress of Toxicology, Belgrade, April 18-21, 2018. 2018;:152-153. https://hdl.handle.net/21.15107/rcub_veterinar_2647 .
Ćupić Miladinović, Dejana, Borozan, Sunčica, Peković, Sanja, Dacić, Sanja, Đukić-Ćosić, Danijela, Ćupić, Vitomir, Ivanović, Saša, "The effect of thiamine on activity of enzymes (with a special emphasis on MAPK) in the brain of Japanese quails treated with chlorpyrifos" in 10th Congress of Toxicology in Developing Countries (CTDC 10) and the 12th Serbian Congress of Toxicology, Belgrade, April 18-21, 2018 (2018):152-153, https://hdl.handle.net/21.15107/rcub_veterinar_2647 .