Biofilm Production and Antimicrobial Resistance of Clinical and Food Isolates of Pseudomonas spp.
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2020
Authors
Savić Radovanović, RadoslavaRajić Savić, Nataša
Ranin, Lazar
Smitran, Aleksandra
Vučković Opavski, Nataša
Milosavljević Tepavčević, Andreja
Ranin, Jovana
Gajić, Ina
Article (Published version)
,
Springer
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Show full item recordAbstract
Due to its ubiquity, ability to form biofilms, and acquire resistance mechanisms, Pseudomonas spp. become one of the major
challenge for healthcare settings and food industry. The aims of this study were to assess the biofilm production of Pseudomonas
spp. recovered from clinical and food specimens and to evaluate their antimicrobial resistance. A total of 108
isolates of Pseudomonas spp. were included in the study, 48 being clinical isolates recovered from patients admitted to four
tertiary care hospitals throughout Serbia and 60 were isolated from the bulk tank milk samples and meat carcasses. Biofilm
production was analyzed by microtiter plate assay. Antimicrobial susceptibility was evaluated by disk diffusion method
according to the CLSI guidelines, while class A and B β-lactamases encoding genes were screened by PCR. A total of 98
(90.7%) strains were biofilm producers (moderate producer: 68, 69.4%; strong producer: 8, 8.2%). Although a slightly higher
percentage of clinic...al isolates were biofilm producers (91.7%) compared to food isolates (90%), statistical significance was
not observed (P > 0.05). The proportion of carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates was significantly
higher among clinical (42%) isolates compared to food (1.7%) Pseudomonads (P < 0.05). The blaPER and blaNDM genes were
found in ESBL (seven isolates) and MBL (two isolates) production, respectively. In the present study, we confirmed that
biofilm formation was highly present in both clinical and food Pseudomonas spp. irrespective of the prior existence of resistance
genes. Additionally, clinical settings pose a major reservoir of MDR Pseudomonas spp. and especially CRPA isolates.
Source:
Current Microbiology, 2020, 77, 4045-4052Publisher:
- Springer
Funding / projects:
- Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46009)
- Antibiotic resistant bacterial pathogens in Serbia: phenotypic and genotypic characterization (RS-MESTD-Basic Research (BR or ON)-175039)
Note:
- We gratefully acknowledge the following persons for supplying the strains used in this study: Snezana Jovanovic, Bojan Rakonjac, Momir Djuric, Lidija Boskovic, Teodora Vitorovic, Snezana Delic, Branislava Kocic, Dragana Andjelkovic.
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Fakultet veterinarske medicineTY - JOUR AU - Savić Radovanović, Radoslava AU - Rajić Savić, Nataša AU - Ranin, Lazar AU - Smitran, Aleksandra AU - Vučković Opavski, Nataša AU - Milosavljević Tepavčević, Andreja AU - Ranin, Jovana AU - Gajić, Ina PY - 2020 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2697 AB - Due to its ubiquity, ability to form biofilms, and acquire resistance mechanisms, Pseudomonas spp. become one of the major challenge for healthcare settings and food industry. The aims of this study were to assess the biofilm production of Pseudomonas spp. recovered from clinical and food specimens and to evaluate their antimicrobial resistance. A total of 108 isolates of Pseudomonas spp. were included in the study, 48 being clinical isolates recovered from patients admitted to four tertiary care hospitals throughout Serbia and 60 were isolated from the bulk tank milk samples and meat carcasses. Biofilm production was analyzed by microtiter plate assay. Antimicrobial susceptibility was evaluated by disk diffusion method according to the CLSI guidelines, while class A and B β-lactamases encoding genes were screened by PCR. A total of 98 (90.7%) strains were biofilm producers (moderate producer: 68, 69.4%; strong producer: 8, 8.2%). Although a slightly higher percentage of clinical isolates were biofilm producers (91.7%) compared to food isolates (90%), statistical significance was not observed (P > 0.05). The proportion of carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates was significantly higher among clinical (42%) isolates compared to food (1.7%) Pseudomonads (P < 0.05). The blaPER and blaNDM genes were found in ESBL (seven isolates) and MBL (two isolates) production, respectively. In the present study, we confirmed that biofilm formation was highly present in both clinical and food Pseudomonas spp. irrespective of the prior existence of resistance genes. Additionally, clinical settings pose a major reservoir of MDR Pseudomonas spp. and especially CRPA isolates. PB - Springer T2 - Current Microbiology T1 - Biofilm Production and Antimicrobial Resistance of Clinical and Food Isolates of Pseudomonas spp. VL - 77 SP - 4045 EP - 4052 DO - 10.1007/s00284-020-02236-4 ER -
@article{ author = "Savić Radovanović, Radoslava and Rajić Savić, Nataša and Ranin, Lazar and Smitran, Aleksandra and Vučković Opavski, Nataša and Milosavljević Tepavčević, Andreja and Ranin, Jovana and Gajić, Ina", year = "2020", abstract = "Due to its ubiquity, ability to form biofilms, and acquire resistance mechanisms, Pseudomonas spp. become one of the major challenge for healthcare settings and food industry. The aims of this study were to assess the biofilm production of Pseudomonas spp. recovered from clinical and food specimens and to evaluate their antimicrobial resistance. A total of 108 isolates of Pseudomonas spp. were included in the study, 48 being clinical isolates recovered from patients admitted to four tertiary care hospitals throughout Serbia and 60 were isolated from the bulk tank milk samples and meat carcasses. Biofilm production was analyzed by microtiter plate assay. Antimicrobial susceptibility was evaluated by disk diffusion method according to the CLSI guidelines, while class A and B β-lactamases encoding genes were screened by PCR. A total of 98 (90.7%) strains were biofilm producers (moderate producer: 68, 69.4%; strong producer: 8, 8.2%). Although a slightly higher percentage of clinical isolates were biofilm producers (91.7%) compared to food isolates (90%), statistical significance was not observed (P > 0.05). The proportion of carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates was significantly higher among clinical (42%) isolates compared to food (1.7%) Pseudomonads (P < 0.05). The blaPER and blaNDM genes were found in ESBL (seven isolates) and MBL (two isolates) production, respectively. In the present study, we confirmed that biofilm formation was highly present in both clinical and food Pseudomonas spp. irrespective of the prior existence of resistance genes. Additionally, clinical settings pose a major reservoir of MDR Pseudomonas spp. and especially CRPA isolates.", publisher = "Springer", journal = "Current Microbiology", title = "Biofilm Production and Antimicrobial Resistance of Clinical and Food Isolates of Pseudomonas spp.", volume = "77", pages = "4045-4052", doi = "10.1007/s00284-020-02236-4" }
Savić Radovanović, R., Rajić Savić, N., Ranin, L., Smitran, A., Vučković Opavski, N., Milosavljević Tepavčević, A., Ranin, J.,& Gajić, I.. (2020). Biofilm Production and Antimicrobial Resistance of Clinical and Food Isolates of Pseudomonas spp.. in Current Microbiology Springer., 77, 4045-4052. https://doi.org/10.1007/s00284-020-02236-4
Savić Radovanović R, Rajić Savić N, Ranin L, Smitran A, Vučković Opavski N, Milosavljević Tepavčević A, Ranin J, Gajić I. Biofilm Production and Antimicrobial Resistance of Clinical and Food Isolates of Pseudomonas spp.. in Current Microbiology. 2020;77:4045-4052. doi:10.1007/s00284-020-02236-4 .
Savić Radovanović, Radoslava, Rajić Savić, Nataša, Ranin, Lazar, Smitran, Aleksandra, Vučković Opavski, Nataša, Milosavljević Tepavčević, Andreja, Ranin, Jovana, Gajić, Ina, "Biofilm Production and Antimicrobial Resistance of Clinical and Food Isolates of Pseudomonas spp." in Current Microbiology, 77 (2020):4045-4052, https://doi.org/10.1007/s00284-020-02236-4 . .