Antimicrobial activity of cinnamaldehyde, carvacrol, p-cymene and oregano (Origanum vulgare) essential oil against Staphylococcus aureus and Escherichia coli
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Use of plant extracts is considered to be natural and effective preservation strategies for meat and dairy products since different essential oils (EOs) and their compounds exhibited strong antibacterial activity against pathogenic and spoilage bacteria in food model systems. Essential oils are volatile mixtures of different compounds and a number of these compounds exhibited significant antimicrobial properties when tested separately. Nevertheless, there are no sufficient data about the combined effect of these compounds. In the present study cinnamaldehyde, carvacrol, p-cymene, oregano essential oil and their combinations were evaluated for antibacterial properties against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. Antibacterial activity was determined by measuring the minimum inhibitory concentration (MIC). Both active compounds carvacrol and cinnamaldehyde exhibited great antibacterial activity against bacteria used in this test, while p-cymene did not exhibi...t antibacterial effect (MIC>5000 g/ml). The MlC of carvacrol was same for both bacteria (320 g/ml) while the MIC of cinnamaldehyde was 320 and 160 g/ml for E. coli and S. aureus. The antibacterial actir.ity of oregano EO shows an inhibition effect against E. coli and S. aureus with MIC of 320 and 640 g/ml. Although generally EOs are more effective against Gram-positive bacteria the results of the present study showed that both bacteria were equally sensitive to tested antibacterial agents with an exception of cinnamaldehyde that showed stronger antibacterial activity against S.aureus than against E. coli. However, mixtures of cinnamaldehyde with carvacrol did not show synergistic effects against E. coli and S. aureus. When used in combination of cinnamaldehyde 3% and carvacrol 2% antagonistic effect was observed (MIC=640 g/ml), while the combination of cinnamaldehyde in higher concentration (4%) and reduced levels of carvacrol (1%) had the same antibacterial effect as when tested separately (MIC=320 g/ml). The same was observed when combination of 4% of cinnamaldehyde and 1% of OEO was used.
Upotreba etarskih ulja predstavlja prirodnu i efikasnu strategiju za konzerviranje
proizvoda od mesa i mleka. Različita etarska ulja, kao i njihove aktivne
komponente pokazala su antimikrobnu aktivnost prema velikom broju patogenih i
bakterija uzrokovača kvara. Etarska ulja su volatilne smeše hemijski različitih
jedinjenja. Veliki broj ovih jedinjenja poseduje antimikrobe osobine. Međutim,
nema dovoljno podataka u literaturi o njihovom kombinovanom efektu. U ovom
eksperimentu ispitavan je uticaj cinamaldehida, karvakrola, p-cimena, etarskog
ulja origana i njihovih mešavina na Escherichia coli ATCC 25922 i Staphylococcus
aureus ATCC 25923. Za ispitivanje antimikrobne aktivnosti etarskih ulja korišćena
je metoda mikrodilucije i određene su minimalne inhibitorne koncentracije (MIC).
Karvakrol i cinamaldehid pokazali su snažnu antibakterijsku aktivnost, dok pcymene nije ostvario antimikroban efekat (MIC>5000 g/ml). MIC karvakrola bila
je ista za obe testirane bakterije (320 g/ml...) dok je MIC cinamaldehida iznosila 320
g/ml za E. coli i 160 g/ml za S. aureus. Iako su Gram-pozitivne bakterije generalno
osetljivije na dejstvo etarskih ulja, rezultati ovog ogleda pokazuju da su obe
bakterije bile jednako osetljive na dejstvo svih ispitanih supstanci, sa izuzetkom
cinamaldehida koji je ispoljio jaču antimikrobnu aktivnost prema S. aureus. Ipak,
mešavine cinamaldehida i karvakrola nisu ispoljile sinergistički efekat prema
testiranim bakterijama. Kombinacija 3% cinamaldehida i 2% karvakrola ispoljila je
antagonistički efekat (MIC=640 g/ml), dok je kombinacija cinamaldehida u višoj
koncentraciji (4%) i karvakrola u nižoj koncentraciji (1%) imala isti MIC kao ove
supstance testirane zasebno (MIC=320 g/ml). Isti rezultati dobijeni su i kada je
korišćena smeša 4% cinamaldehida i 1% etarskog ulja origana
Кључне речи:
phenols / antibacterial activity / MIC / fenolne komponente / antibakterijska aktivnost / MICИзвор:
24. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske/BIH, međunarodni naučni skup, Bijeljina 12-15. jun 2019, 2019, 155-156Издавач:
- Banja Luka : Veterinarska komora Republike Srpske
Финансирање / пројекти:
- Одабране биолошке опасности за безбедност/квалитет хране анималног порекла и контролне мере од фарме до потрошача (RS-MESTD-Technological Development (TD or TR)-31034)
Напомена:
- Zbornik kratkih sadržaja
Колекције
Институција/група
Fakultet veterinarske medicineTY - CONF AU - Bošković, Marija AU - Zdravković, Nemanja AU - Glišić, Milica AU - Babić, Milijana AU - Baltić, Milan Ž. PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2976 AB - Use of plant extracts is considered to be natural and effective preservation strategies for meat and dairy products since different essential oils (EOs) and their compounds exhibited strong antibacterial activity against pathogenic and spoilage bacteria in food model systems. Essential oils are volatile mixtures of different compounds and a number of these compounds exhibited significant antimicrobial properties when tested separately. Nevertheless, there are no sufficient data about the combined effect of these compounds. In the present study cinnamaldehyde, carvacrol, p-cymene, oregano essential oil and their combinations were evaluated for antibacterial properties against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. Antibacterial activity was determined by measuring the minimum inhibitory concentration (MIC). Both active compounds carvacrol and cinnamaldehyde exhibited great antibacterial activity against bacteria used in this test, while p-cymene did not exhibit antibacterial effect (MIC>5000 g/ml). The MlC of carvacrol was same for both bacteria (320 g/ml) while the MIC of cinnamaldehyde was 320 and 160 g/ml for E. coli and S. aureus. The antibacterial actir.ity of oregano EO shows an inhibition effect against E. coli and S. aureus with MIC of 320 and 640 g/ml. Although generally EOs are more effective against Gram-positive bacteria the results of the present study showed that both bacteria were equally sensitive to tested antibacterial agents with an exception of cinnamaldehyde that showed stronger antibacterial activity against S.aureus than against E. coli. However, mixtures of cinnamaldehyde with carvacrol did not show synergistic effects against E. coli and S. aureus. When used in combination of cinnamaldehyde 3% and carvacrol 2% antagonistic effect was observed (MIC=640 g/ml), while the combination of cinnamaldehyde in higher concentration (4%) and reduced levels of carvacrol (1%) had the same antibacterial effect as when tested separately (MIC=320 g/ml). The same was observed when combination of 4% of cinnamaldehyde and 1% of OEO was used. AB - Upotreba etarskih ulja predstavlja prirodnu i efikasnu strategiju za konzerviranje proizvoda od mesa i mleka. Različita etarska ulja, kao i njihove aktivne komponente pokazala su antimikrobnu aktivnost prema velikom broju patogenih i bakterija uzrokovača kvara. Etarska ulja su volatilne smeše hemijski različitih jedinjenja. Veliki broj ovih jedinjenja poseduje antimikrobe osobine. Međutim, nema dovoljno podataka u literaturi o njihovom kombinovanom efektu. U ovom eksperimentu ispitavan je uticaj cinamaldehida, karvakrola, p-cimena, etarskog ulja origana i njihovih mešavina na Escherichia coli ATCC 25922 i Staphylococcus aureus ATCC 25923. Za ispitivanje antimikrobne aktivnosti etarskih ulja korišćena je metoda mikrodilucije i određene su minimalne inhibitorne koncentracije (MIC). Karvakrol i cinamaldehid pokazali su snažnu antibakterijsku aktivnost, dok pcymene nije ostvario antimikroban efekat (MIC>5000 g/ml). MIC karvakrola bila je ista za obe testirane bakterije (320 g/ml) dok je MIC cinamaldehida iznosila 320 g/ml za E. coli i 160 g/ml za S. aureus. Iako su Gram-pozitivne bakterije generalno osetljivije na dejstvo etarskih ulja, rezultati ovog ogleda pokazuju da su obe bakterije bile jednako osetljive na dejstvo svih ispitanih supstanci, sa izuzetkom cinamaldehida koji je ispoljio jaču antimikrobnu aktivnost prema S. aureus. Ipak, mešavine cinamaldehida i karvakrola nisu ispoljile sinergistički efekat prema testiranim bakterijama. Kombinacija 3% cinamaldehida i 2% karvakrola ispoljila je antagonistički efekat (MIC=640 g/ml), dok je kombinacija cinamaldehida u višoj koncentraciji (4%) i karvakrola u nižoj koncentraciji (1%) imala isti MIC kao ove supstance testirane zasebno (MIC=320 g/ml). Isti rezultati dobijeni su i kada je korišćena smeša 4% cinamaldehida i 1% etarskog ulja origana PB - Banja Luka : Veterinarska komora Republike Srpske C3 - 24. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske/BIH, međunarodni naučni skup, Bijeljina 12-15. jun 2019 T1 - Antimicrobial activity of cinnamaldehyde, carvacrol, p-cymene and oregano (Origanum vulgare) essential oil against Staphylococcus aureus and Escherichia coli SP - 155 EP - 156 UR - https://hdl.handle.net/21.15107/rcub_veterinar_2976 ER -
@conference{ author = "Bošković, Marija and Zdravković, Nemanja and Glišić, Milica and Babić, Milijana and Baltić, Milan Ž.", year = "2019", abstract = "Use of plant extracts is considered to be natural and effective preservation strategies for meat and dairy products since different essential oils (EOs) and their compounds exhibited strong antibacterial activity against pathogenic and spoilage bacteria in food model systems. Essential oils are volatile mixtures of different compounds and a number of these compounds exhibited significant antimicrobial properties when tested separately. Nevertheless, there are no sufficient data about the combined effect of these compounds. In the present study cinnamaldehyde, carvacrol, p-cymene, oregano essential oil and their combinations were evaluated for antibacterial properties against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. Antibacterial activity was determined by measuring the minimum inhibitory concentration (MIC). Both active compounds carvacrol and cinnamaldehyde exhibited great antibacterial activity against bacteria used in this test, while p-cymene did not exhibit antibacterial effect (MIC>5000 g/ml). The MlC of carvacrol was same for both bacteria (320 g/ml) while the MIC of cinnamaldehyde was 320 and 160 g/ml for E. coli and S. aureus. The antibacterial actir.ity of oregano EO shows an inhibition effect against E. coli and S. aureus with MIC of 320 and 640 g/ml. Although generally EOs are more effective against Gram-positive bacteria the results of the present study showed that both bacteria were equally sensitive to tested antibacterial agents with an exception of cinnamaldehyde that showed stronger antibacterial activity against S.aureus than against E. coli. However, mixtures of cinnamaldehyde with carvacrol did not show synergistic effects against E. coli and S. aureus. When used in combination of cinnamaldehyde 3% and carvacrol 2% antagonistic effect was observed (MIC=640 g/ml), while the combination of cinnamaldehyde in higher concentration (4%) and reduced levels of carvacrol (1%) had the same antibacterial effect as when tested separately (MIC=320 g/ml). The same was observed when combination of 4% of cinnamaldehyde and 1% of OEO was used., Upotreba etarskih ulja predstavlja prirodnu i efikasnu strategiju za konzerviranje proizvoda od mesa i mleka. Različita etarska ulja, kao i njihove aktivne komponente pokazala su antimikrobnu aktivnost prema velikom broju patogenih i bakterija uzrokovača kvara. Etarska ulja su volatilne smeše hemijski različitih jedinjenja. Veliki broj ovih jedinjenja poseduje antimikrobe osobine. Međutim, nema dovoljno podataka u literaturi o njihovom kombinovanom efektu. U ovom eksperimentu ispitavan je uticaj cinamaldehida, karvakrola, p-cimena, etarskog ulja origana i njihovih mešavina na Escherichia coli ATCC 25922 i Staphylococcus aureus ATCC 25923. Za ispitivanje antimikrobne aktivnosti etarskih ulja korišćena je metoda mikrodilucije i određene su minimalne inhibitorne koncentracije (MIC). Karvakrol i cinamaldehid pokazali su snažnu antibakterijsku aktivnost, dok pcymene nije ostvario antimikroban efekat (MIC>5000 g/ml). MIC karvakrola bila je ista za obe testirane bakterije (320 g/ml) dok je MIC cinamaldehida iznosila 320 g/ml za E. coli i 160 g/ml za S. aureus. Iako su Gram-pozitivne bakterije generalno osetljivije na dejstvo etarskih ulja, rezultati ovog ogleda pokazuju da su obe bakterije bile jednako osetljive na dejstvo svih ispitanih supstanci, sa izuzetkom cinamaldehida koji je ispoljio jaču antimikrobnu aktivnost prema S. aureus. Ipak, mešavine cinamaldehida i karvakrola nisu ispoljile sinergistički efekat prema testiranim bakterijama. Kombinacija 3% cinamaldehida i 2% karvakrola ispoljila je antagonistički efekat (MIC=640 g/ml), dok je kombinacija cinamaldehida u višoj koncentraciji (4%) i karvakrola u nižoj koncentraciji (1%) imala isti MIC kao ove supstance testirane zasebno (MIC=320 g/ml). Isti rezultati dobijeni su i kada je korišćena smeša 4% cinamaldehida i 1% etarskog ulja origana", publisher = "Banja Luka : Veterinarska komora Republike Srpske", journal = "24. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske/BIH, međunarodni naučni skup, Bijeljina 12-15. jun 2019", title = "Antimicrobial activity of cinnamaldehyde, carvacrol, p-cymene and oregano (Origanum vulgare) essential oil against Staphylococcus aureus and Escherichia coli", pages = "155-156", url = "https://hdl.handle.net/21.15107/rcub_veterinar_2976" }
Bošković, M., Zdravković, N., Glišić, M., Babić, M.,& Baltić, M. Ž.. (2019). Antimicrobial activity of cinnamaldehyde, carvacrol, p-cymene and oregano (Origanum vulgare) essential oil against Staphylococcus aureus and Escherichia coli. in 24. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske/BIH, međunarodni naučni skup, Bijeljina 12-15. jun 2019 Banja Luka : Veterinarska komora Republike Srpske., 155-156. https://hdl.handle.net/21.15107/rcub_veterinar_2976
Bošković M, Zdravković N, Glišić M, Babić M, Baltić MŽ. Antimicrobial activity of cinnamaldehyde, carvacrol, p-cymene and oregano (Origanum vulgare) essential oil against Staphylococcus aureus and Escherichia coli. in 24. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske/BIH, međunarodni naučni skup, Bijeljina 12-15. jun 2019. 2019;:155-156. https://hdl.handle.net/21.15107/rcub_veterinar_2976 .
Bošković, Marija, Zdravković, Nemanja, Glišić, Milica, Babić, Milijana, Baltić, Milan Ž., "Antimicrobial activity of cinnamaldehyde, carvacrol, p-cymene and oregano (Origanum vulgare) essential oil against Staphylococcus aureus and Escherichia coli" in 24. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske/BIH, međunarodni naučni skup, Bijeljina 12-15. jun 2019 (2019):155-156, https://hdl.handle.net/21.15107/rcub_veterinar_2976 .