Mutagenic activity of estradiol evaluated by an in vitro micronucleus assay - Short communication
Abstract
The objective of the present study was to evaluate possible genetic changes in cultured human lymphocytes treated with estradiol, using the cytokinesis block micronucleus assay. Eight experimental concentrations of estradiol were used (range from 10(-10) M to 0.7 x 10(-4) M). The obtained results indicate that estradiol exhibits aneugenic and/or clastogenic effects, expressed as increased frequency of micronucleated lymphocytes at two highest experimental concentrations used in this investigation. In addition to genotoxic effects, these concentrations decreased the cytokinesis block proliferation index (CBPI) and percentage of binucleated cells, indicating the cell cycle delay and possible cytotoxic effects. In conclusion, estradiol treatment might represent a human health risk, especially if overdosed or used for a prolonged period of time.
Keywords:
estradio / micronucleus / mutagen / cancer / lymphocyteSource:
Acta Biologica Hungarica, 2005, 56, 3-4, 403-406Publisher:
- Akademiai Kiado, Budapest
DOI: 10.1556/ABiol.56.2005.3-4.22
ISSN: 0236-5383
PubMed: 16196214
WoS: 000231917900022
Scopus: 2-s2.0-24144464811
Collections
Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Đelić, Ninoslav AU - Spremo-Potparević, Biljana AU - Đelić, D PY - 2005 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/340 AB - The objective of the present study was to evaluate possible genetic changes in cultured human lymphocytes treated with estradiol, using the cytokinesis block micronucleus assay. Eight experimental concentrations of estradiol were used (range from 10(-10) M to 0.7 x 10(-4) M). The obtained results indicate that estradiol exhibits aneugenic and/or clastogenic effects, expressed as increased frequency of micronucleated lymphocytes at two highest experimental concentrations used in this investigation. In addition to genotoxic effects, these concentrations decreased the cytokinesis block proliferation index (CBPI) and percentage of binucleated cells, indicating the cell cycle delay and possible cytotoxic effects. In conclusion, estradiol treatment might represent a human health risk, especially if overdosed or used for a prolonged period of time. PB - Akademiai Kiado, Budapest T2 - Acta Biologica Hungarica T1 - Mutagenic activity of estradiol evaluated by an in vitro micronucleus assay - Short communication VL - 56 IS - 3-4 SP - 403 EP - 406 DO - 10.1556/ABiol.56.2005.3-4.22 ER -
@article{ author = "Đelić, Ninoslav and Spremo-Potparević, Biljana and Đelić, D", year = "2005", abstract = "The objective of the present study was to evaluate possible genetic changes in cultured human lymphocytes treated with estradiol, using the cytokinesis block micronucleus assay. Eight experimental concentrations of estradiol were used (range from 10(-10) M to 0.7 x 10(-4) M). The obtained results indicate that estradiol exhibits aneugenic and/or clastogenic effects, expressed as increased frequency of micronucleated lymphocytes at two highest experimental concentrations used in this investigation. In addition to genotoxic effects, these concentrations decreased the cytokinesis block proliferation index (CBPI) and percentage of binucleated cells, indicating the cell cycle delay and possible cytotoxic effects. In conclusion, estradiol treatment might represent a human health risk, especially if overdosed or used for a prolonged period of time.", publisher = "Akademiai Kiado, Budapest", journal = "Acta Biologica Hungarica", title = "Mutagenic activity of estradiol evaluated by an in vitro micronucleus assay - Short communication", volume = "56", number = "3-4", pages = "403-406", doi = "10.1556/ABiol.56.2005.3-4.22" }
Đelić, N., Spremo-Potparević, B.,& Đelić, D.. (2005). Mutagenic activity of estradiol evaluated by an in vitro micronucleus assay - Short communication. in Acta Biologica Hungarica Akademiai Kiado, Budapest., 56(3-4), 403-406. https://doi.org/10.1556/ABiol.56.2005.3-4.22
Đelić N, Spremo-Potparević B, Đelić D. Mutagenic activity of estradiol evaluated by an in vitro micronucleus assay - Short communication. in Acta Biologica Hungarica. 2005;56(3-4):403-406. doi:10.1556/ABiol.56.2005.3-4.22 .
Đelić, Ninoslav, Spremo-Potparević, Biljana, Đelić, D, "Mutagenic activity of estradiol evaluated by an in vitro micronucleus assay - Short communication" in Acta Biologica Hungarica, 56, no. 3-4 (2005):403-406, https://doi.org/10.1556/ABiol.56.2005.3-4.22 . .