Pharmacological effects of monoterpene carveol on the neuromuscular system of nematodes and mammals
Аутори
Stojković, MajaTodorović, Zoran
Protić, Dragana
Stevanović, Strahinja
Medić, Dragana
Charvet, Claude L.
Marjanović, Djordje
Nedeljković Trailović, Jelena
Trailović, Saša M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The control of parasitic nematode infections relies mostly on anthelmintics. The potential pharmacotherapeutic application of phytochemicals, in order to overcome parasite resistance and enhance the effect of existing drugs, is becoming increasingly important. The antinematodal effects of carveol was tested on the free-living nematode Caenorhabditis elegans and the neuromuscular preparation of the parasitic nematode Ascaris suum. Carveol caused spastic and geraniol atonic paralysis in C. elegans. In A. suum carveol potentiated contractions induced by acetylcholine (ACh) and this effect was confirmed with two-electrode voltage-clamp electrophysiology on the A. suum nicotinic acetylcholine receptor expressed in Xenopus oocytes. However, potentiating effect of carveol on ACh-induced contractions was partially sensitive to atropine, indicates a dominant nicotine effect but also the involvement of some muscarinic structures. The effects of carveol on the neuromuscular system of mammals are ...also specific. In micromolar concentrations, carveol acts as a non-competitive ACh antagonist on ileum contractions. Unlike atropine, it does not change the EC50 of ACh but reduces the amplitude of contractions. Carveol caused an increase in Electrical Field Stimulation-evoked contractions of the isolated rat diaphragm, but at higher concentrations it caused an inhibition. Also, carveol neutralized the mecamylamine-induced tetanic fade, indicating a possibly different pre-and post-synaptic action at the neuromuscular junction.
Кључне речи:
carveol / C. elegans / Xenopus oocytes / A. suum / AChRИзвор:
Frontiers in Pharmacology, 2024, 15Издавач:
- Frontiers Media
Финансирање / пројекти:
- NIH Office of Research Infrastructure Programs (P40 OD010440)
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Stojković, Maja AU - Todorović, Zoran AU - Protić, Dragana AU - Stevanović, Strahinja AU - Medić, Dragana AU - Charvet, Claude L. AU - Marjanović, Djordje AU - Nedeljković Trailović, Jelena AU - Trailović, Saša M. PY - 2024 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3564 AB - The control of parasitic nematode infections relies mostly on anthelmintics. The potential pharmacotherapeutic application of phytochemicals, in order to overcome parasite resistance and enhance the effect of existing drugs, is becoming increasingly important. The antinematodal effects of carveol was tested on the free-living nematode Caenorhabditis elegans and the neuromuscular preparation of the parasitic nematode Ascaris suum. Carveol caused spastic and geraniol atonic paralysis in C. elegans. In A. suum carveol potentiated contractions induced by acetylcholine (ACh) and this effect was confirmed with two-electrode voltage-clamp electrophysiology on the A. suum nicotinic acetylcholine receptor expressed in Xenopus oocytes. However, potentiating effect of carveol on ACh-induced contractions was partially sensitive to atropine, indicates a dominant nicotine effect but also the involvement of some muscarinic structures. The effects of carveol on the neuromuscular system of mammals are also specific. In micromolar concentrations, carveol acts as a non-competitive ACh antagonist on ileum contractions. Unlike atropine, it does not change the EC50 of ACh but reduces the amplitude of contractions. Carveol caused an increase in Electrical Field Stimulation-evoked contractions of the isolated rat diaphragm, but at higher concentrations it caused an inhibition. Also, carveol neutralized the mecamylamine-induced tetanic fade, indicating a possibly different pre-and post-synaptic action at the neuromuscular junction. PB - Frontiers Media T2 - Frontiers in Pharmacology T1 - Pharmacological effects of monoterpene carveol on the neuromuscular system of nematodes and mammals VL - 15 DO - 10.3389/fphar.2024.1326779 ER -
@article{ author = "Stojković, Maja and Todorović, Zoran and Protić, Dragana and Stevanović, Strahinja and Medić, Dragana and Charvet, Claude L. and Marjanović, Djordje and Nedeljković Trailović, Jelena and Trailović, Saša M.", year = "2024", abstract = "The control of parasitic nematode infections relies mostly on anthelmintics. The potential pharmacotherapeutic application of phytochemicals, in order to overcome parasite resistance and enhance the effect of existing drugs, is becoming increasingly important. The antinematodal effects of carveol was tested on the free-living nematode Caenorhabditis elegans and the neuromuscular preparation of the parasitic nematode Ascaris suum. Carveol caused spastic and geraniol atonic paralysis in C. elegans. In A. suum carveol potentiated contractions induced by acetylcholine (ACh) and this effect was confirmed with two-electrode voltage-clamp electrophysiology on the A. suum nicotinic acetylcholine receptor expressed in Xenopus oocytes. However, potentiating effect of carveol on ACh-induced contractions was partially sensitive to atropine, indicates a dominant nicotine effect but also the involvement of some muscarinic structures. The effects of carveol on the neuromuscular system of mammals are also specific. In micromolar concentrations, carveol acts as a non-competitive ACh antagonist on ileum contractions. Unlike atropine, it does not change the EC50 of ACh but reduces the amplitude of contractions. Carveol caused an increase in Electrical Field Stimulation-evoked contractions of the isolated rat diaphragm, but at higher concentrations it caused an inhibition. Also, carveol neutralized the mecamylamine-induced tetanic fade, indicating a possibly different pre-and post-synaptic action at the neuromuscular junction.", publisher = "Frontiers Media", journal = "Frontiers in Pharmacology", title = "Pharmacological effects of monoterpene carveol on the neuromuscular system of nematodes and mammals", volume = "15", doi = "10.3389/fphar.2024.1326779" }
Stojković, M., Todorović, Z., Protić, D., Stevanović, S., Medić, D., Charvet, C. L., Marjanović, D., Nedeljković Trailović, J.,& Trailović, S. M.. (2024). Pharmacological effects of monoterpene carveol on the neuromuscular system of nematodes and mammals. in Frontiers in Pharmacology Frontiers Media., 15. https://doi.org/10.3389/fphar.2024.1326779
Stojković M, Todorović Z, Protić D, Stevanović S, Medić D, Charvet CL, Marjanović D, Nedeljković Trailović J, Trailović SM. Pharmacological effects of monoterpene carveol on the neuromuscular system of nematodes and mammals. in Frontiers in Pharmacology. 2024;15. doi:10.3389/fphar.2024.1326779 .
Stojković, Maja, Todorović, Zoran, Protić, Dragana, Stevanović, Strahinja, Medić, Dragana, Charvet, Claude L., Marjanović, Djordje, Nedeljković Trailović, Jelena, Trailović, Saša M., "Pharmacological effects of monoterpene carveol on the neuromuscular system of nematodes and mammals" in Frontiers in Pharmacology, 15 (2024), https://doi.org/10.3389/fphar.2024.1326779 . .