Open days period and antispermatozoal antibodies in artificially inseminated cows
Servis period i antitela protiv spermatozoida kod veštački osemenjavanih krava
2005
Autori
Milovanović, AleksandarLazarević, Miodrag
Milanović, Svetlana
Kirovski, Danijela
Jovičin, Milovan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aim of this study was to determine titers of antisperm antibodies (ASA) of Ig G and Ig A class in sera and cervical mucus of artificially inseminated Holstein cows in order to correlate these results with the duration of the open days period. Investigations were conducted on a total of 181 cows originating from three different dairy farms. Blood and cervical mucus samples for laboratory analyses were collected on the day of the last artificial insemination. Presence of ASA was determined by indirect immunofluorescence method (IIF) using bulls' sperm cells prepared for artificial insemination by suspending in TRIS - egg yolk or "Biociphos + " extenders prior to deep freezing. Our results strongly confirm the hypothesis that immune mechanisms may be involved in reproductive disturbances due to high levels of ASA of lg A class. In the sera and cervical mucus of cows, high levels of ASA were found in animals with longer open days period. In this study we were not able to demonstrate di...fferences in ASA titers when sperm cells were suspended in different extenders and used for the IIF test.
U ovom radu su izneti rezultati ispitivanja titra antitela Ig G i Ig A klase poreklom iz krvnog seruma i cervikalne sluzi veštački osemenjavanih holštajn krava protiv antigena spermatozoida bika. Ispitivanja su izvedena na ukupnom broju od 181 plotkinje sa tri regionalne farme muznih krava. Uzorci krvi i cervikalne sluzi su prikupljeni na dan poslednjeg veštačkog osemenjavanja. Titar antitela protiv antigena spermatozoida (ASA) je određivan metodom indirektne imunofluorescence a za izvođenje testa su korišćeni spermatozoidi suspendovani u TRIS žumanjčanom ili "Biociphos +" razređivaču. Naši rezultati potvrđuju hipotezu da su imunski mehanizmi uključeni u nastanak nekih reproduktivnih poremećaja jer su plotkinje sa visokim titrom ASA u serumu i cervikalnoj sluzi imala duži servis period. Osim toga, u ovim ispitivanjima nismo utvrdili postojanje razlika u titru ASA u zavisnosti od vrste korišćenog razređivača.
Ključne reči:
sperm cells / cows / artificial insemination / sub fertility / antisperm antibodiesIzvor:
Acta Veterinaria-Beograd, 2005, 55, 5-6, 449-460Izdavač:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
DOI: 10.2298/AVB0506449M
ISSN: 0567-8315
WoS: 000234035600011
Scopus: 2-s2.0-29344454333
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Milovanović, Aleksandar AU - Lazarević, Miodrag AU - Milanović, Svetlana AU - Kirovski, Danijela AU - Jovičin, Milovan PY - 2005 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/361 AB - The aim of this study was to determine titers of antisperm antibodies (ASA) of Ig G and Ig A class in sera and cervical mucus of artificially inseminated Holstein cows in order to correlate these results with the duration of the open days period. Investigations were conducted on a total of 181 cows originating from three different dairy farms. Blood and cervical mucus samples for laboratory analyses were collected on the day of the last artificial insemination. Presence of ASA was determined by indirect immunofluorescence method (IIF) using bulls' sperm cells prepared for artificial insemination by suspending in TRIS - egg yolk or "Biociphos + " extenders prior to deep freezing. Our results strongly confirm the hypothesis that immune mechanisms may be involved in reproductive disturbances due to high levels of ASA of lg A class. In the sera and cervical mucus of cows, high levels of ASA were found in animals with longer open days period. In this study we were not able to demonstrate differences in ASA titers when sperm cells were suspended in different extenders and used for the IIF test. AB - U ovom radu su izneti rezultati ispitivanja titra antitela Ig G i Ig A klase poreklom iz krvnog seruma i cervikalne sluzi veštački osemenjavanih holštajn krava protiv antigena spermatozoida bika. Ispitivanja su izvedena na ukupnom broju od 181 plotkinje sa tri regionalne farme muznih krava. Uzorci krvi i cervikalne sluzi su prikupljeni na dan poslednjeg veštačkog osemenjavanja. Titar antitela protiv antigena spermatozoida (ASA) je određivan metodom indirektne imunofluorescence a za izvođenje testa su korišćeni spermatozoidi suspendovani u TRIS žumanjčanom ili "Biociphos +" razređivaču. Naši rezultati potvrđuju hipotezu da su imunski mehanizmi uključeni u nastanak nekih reproduktivnih poremećaja jer su plotkinje sa visokim titrom ASA u serumu i cervikalnoj sluzi imala duži servis period. Osim toga, u ovim ispitivanjima nismo utvrdili postojanje razlika u titru ASA u zavisnosti od vrste korišćenog razređivača. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Open days period and antispermatozoal antibodies in artificially inseminated cows T1 - Servis period i antitela protiv spermatozoida kod veštački osemenjavanih krava VL - 55 IS - 5-6 SP - 449 EP - 460 DO - 10.2298/AVB0506449M ER -
@article{ author = "Milovanović, Aleksandar and Lazarević, Miodrag and Milanović, Svetlana and Kirovski, Danijela and Jovičin, Milovan", year = "2005", abstract = "The aim of this study was to determine titers of antisperm antibodies (ASA) of Ig G and Ig A class in sera and cervical mucus of artificially inseminated Holstein cows in order to correlate these results with the duration of the open days period. Investigations were conducted on a total of 181 cows originating from three different dairy farms. Blood and cervical mucus samples for laboratory analyses were collected on the day of the last artificial insemination. Presence of ASA was determined by indirect immunofluorescence method (IIF) using bulls' sperm cells prepared for artificial insemination by suspending in TRIS - egg yolk or "Biociphos + " extenders prior to deep freezing. Our results strongly confirm the hypothesis that immune mechanisms may be involved in reproductive disturbances due to high levels of ASA of lg A class. In the sera and cervical mucus of cows, high levels of ASA were found in animals with longer open days period. In this study we were not able to demonstrate differences in ASA titers when sperm cells were suspended in different extenders and used for the IIF test., U ovom radu su izneti rezultati ispitivanja titra antitela Ig G i Ig A klase poreklom iz krvnog seruma i cervikalne sluzi veštački osemenjavanih holštajn krava protiv antigena spermatozoida bika. Ispitivanja su izvedena na ukupnom broju od 181 plotkinje sa tri regionalne farme muznih krava. Uzorci krvi i cervikalne sluzi su prikupljeni na dan poslednjeg veštačkog osemenjavanja. Titar antitela protiv antigena spermatozoida (ASA) je određivan metodom indirektne imunofluorescence a za izvođenje testa su korišćeni spermatozoidi suspendovani u TRIS žumanjčanom ili "Biociphos +" razređivaču. Naši rezultati potvrđuju hipotezu da su imunski mehanizmi uključeni u nastanak nekih reproduktivnih poremećaja jer su plotkinje sa visokim titrom ASA u serumu i cervikalnoj sluzi imala duži servis period. Osim toga, u ovim ispitivanjima nismo utvrdili postojanje razlika u titru ASA u zavisnosti od vrste korišćenog razređivača.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Open days period and antispermatozoal antibodies in artificially inseminated cows, Servis period i antitela protiv spermatozoida kod veštački osemenjavanih krava", volume = "55", number = "5-6", pages = "449-460", doi = "10.2298/AVB0506449M" }
Milovanović, A., Lazarević, M., Milanović, S., Kirovski, D.,& Jovičin, M.. (2005). Open days period and antispermatozoal antibodies in artificially inseminated cows. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 55(5-6), 449-460. https://doi.org/10.2298/AVB0506449M
Milovanović A, Lazarević M, Milanović S, Kirovski D, Jovičin M. Open days period and antispermatozoal antibodies in artificially inseminated cows. in Acta Veterinaria-Beograd. 2005;55(5-6):449-460. doi:10.2298/AVB0506449M .
Milovanović, Aleksandar, Lazarević, Miodrag, Milanović, Svetlana, Kirovski, Danijela, Jovičin, Milovan, "Open days period and antispermatozoal antibodies in artificially inseminated cows" in Acta Veterinaria-Beograd, 55, no. 5-6 (2005):449-460, https://doi.org/10.2298/AVB0506449M . .