dc.creator | Milojević, Lazar | |
dc.creator | Velebit, Branko | |
dc.creator | Janković, Vesna | |
dc.creator | Mitrović, Radmila | |
dc.creator | Betić, Nikola | |
dc.creator | Simunović, Sara | |
dc.creator | Dimitrijević, Mirjana | |
dc.date.accessioned | 2024-02-22T10:18:44Z | |
dc.date.available | 2024-02-22T10:18:44Z | |
dc.date.issued | 2024 | |
dc.identifier.issn | 2076-2615 | |
dc.identifier.uri | https://vet-erinar.vet.bg.ac.rs/handle/123456789/3705 | |
dc.description.abstract | The goal of this study conducted in Serbia was to detect HEV in pig liver samples from
slaughterhouses, retail outlets, and environmental swabs over the course of a year. All positive
HEV samples were measured and expressed as HEV gene copy numbers per gram of sample, and a
representative number of samples were sequenced using the Sanger approach. A total of 45 HEVpositive samples were re-amplified using nested RT-PCR employing CODEHOP primers targeting
ORF2 (493 nucleotides). The average prevalence of the HEV genotype 3 in all pig liver samples from
the slaughterhouses was 29%, while HEV prevalence was 44% in liver samples from animals younger
than 3 months. HEV RNA was found in thirteen out of sixty (22%) environmental swab samples
that were taken from different surfaces along the slaughter line. Our findings confirmed seasonal
patterns in HEV prevalence, with two picks (summer and winter periods) during the one-year
examination. Among HEV-positive samples, the average viral particles for all positive liver samples
was 4.41 ± 1.69 log10 genome copies per gram. Phylogenetic analysis revealed the majority of HEV
strains (43/45) from Serbia were grouped in the HEV-3a subtype, while two strains were classified
into the HEV-3c subtype, and one strain could not be classified into any of the HEV-3 subtypes. | sr |
dc.language.iso | en | sr |
dc.publisher | MDPI | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200050/RS// | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Animals | sr |
dc.subject | foodborne viruses | sr |
dc.subject | hepatitis E virus | sr |
dc.subject | zoonosis | sr |
dc.subject | slaughterhouse | sr |
dc.subject | Serbia | sr |
dc.title | Prevalence, genetic diversity, and quantification of the RNA genome of the hepatitis E virus in slaughtered pigs in Serbia | sr |
dc.type | article | sr |
dc.rights.license | BY | sr |
dc.citation.volume | 14 | |
dc.citation.issue | 4 | |
dc.citation.spage | 586 | |
dc.identifier.doi | 10.3390/ani14040586 | |
dc.identifier.fulltext | http://veterinar.vet.bg.ac.rs/bitstream/id/11083/bitstream_11083.pdf | |
dc.type.version | publishedVersion | sr |