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Molekularno-genetička detekcija i identifikacija uzročnika mikrosporidijalnih i virusnih infekcija zastupljenih kod pčelinjih društava na teritoriji Srbije
Molecular detection and identification of microsporidia and viruses in honey bee colonies in Serbia
dc.contributor.advisor | Stanimirović, Zoran | |
dc.contributor.advisor | Vidanović, Dejan | |
dc.contributor.other | Radojičić, Sonja | |
dc.contributor.other | Stevanović, Jevrosima | |
dc.contributor.other | Nišavić, Jakov | |
dc.creator | Simeunović, Predrag R. | |
dc.date.accessioned | 2024-03-27T11:58:11Z | |
dc.date.available | 2024-03-27T11:58:11Z | |
dc.date.issued | 2015 | |
dc.identifier.uri | https://nardus.mpn.gov.rs/handle/123456789/5111 | |
dc.identifier.uri | http://eteze.bg.ac.rs/application/showtheses?thesesId=2743 | |
dc.identifier.uri | https://fedorabg.bg.ac.rs/fedora/get/o:10885/bdef:Content/download | |
dc.identifier.uri | http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=47672335 | |
dc.identifier.uri | https://vet-erinar.vet.bg.ac.rs/handle/123456789/3787 | |
dc.description.abstract | Primena molekularno – genetičkih metoda zauzima značajno mesto udijagnostici oboljenja kod životinja izazvanih delovanjem različitih vrstauzročnika. Ove metode, uglavnom zasnovane na lančanoj reakciji polimeraze(PCR), karakteriše zadovoljavajuća brzina izvođenja i visok stepen specifičnosti,odnosno osetljivosti.Mikrosporidije pčela, Nosema ceranae i Nosema apis, ispoljavanjempatogenog efekta na individualnom nivou, mogu dovesti do gubitka čitavihpčelinjih društava. Umnožavanjem mikrosporidijalne DNK, PCR tehnikaomogućava istovremeno utvrđivanje prisustva i identifikaciju uzročnika uuzorku u kojem mikroskopskim pregledom nije utvrđeno prisustvo spora.Pojedine vrste virusa mogu biti uzročnici poremećaja zdravstvenog stanja pčela.Među najznačajnije viruse koji mogu naneti štete pčelinjim društvima ubrajajuse virus deformisanih krila (DWV), virus akutne paralize pčela (ABPV), virusmešinastog legla (SBV) i virus hronične paralize pčela (CBPV). Pomenuti virusičesto izazivaju inaparentne infekcije koje, usled delovanja nespecifičnih faktorai drugih patogena, za kratko vreme mogu prerasti u klinički manifestne idovesti do gubitka pčelinjih društava. Primenom tehnike Real-time RT-PCRbazirane na TaqMan probama, moguće je otkriti prisustvo virusne RNK koja senalazi u niskoj koncentraciji u ispitanom uzorku. Ovo ima veliki značaj udijagnostici virusnih infekcija kod pčelinjih društava koja ne ispoljavaju kliničkesimptoma bolesti.Cilј ove doktorske disertacije je da se primenom molekularno-genetičkihmetoda izvrši detekcija i identifikacija uzročnika četiri najčešće virusne infekcije(DWV, ABPV, SBV i CBPV) zastuplјene kod pčelinjih zajednica različite jačinekoje potiču iz različitih krajeva Srbije... | sr |
dc.description.abstract | Molecular diagnostic in modern veterinary medicine plays an importantrole in diagnosis of animal health disorders. Major advantages of moleculardiagnostic are quickness and high specificity and sensitivity in detection andidentification of infectious and non-infectious agents. Microsporidia, Nosemaceranae and Nosema apis, as a consequence of pathogenic effects in individualbees may provoke losses of entire honey bee colonies. Amplification ofmicrosporidial DNA using PCR allows simultaneous detection andidentification of Nosema species in samples that appeared to be free fromNosema spores after microscopic examination.Honey bee viruses may have asignificant impact on health status of honey bee colonies. Most commonviruses considered to be able to cause damage to bee colonies include Deformedwing virus (DWV), Chronic bee paralysis virus (CBPV), Acute bee paralysisvirus (ABPV) and Sacbrood virus (SBV). These viruses may exist in colonies inlow infectious titres causing asymptomatic infection that due to the negativeimpact of non-specific factors and/or other pathogens may develop in to covertinfection and lead to colony loss.High specificity and sensitivity of Real-timeRT-PCR method based on TaqMan probes enables detection of very smallamounts of viral RNA in bee samples originated from asymptomatic colonieswhich is advantage of this method compared to end point PCR.The aim of this work is detection and identification of four most commonviruses (DWV, ABPV, SBV and CBPV) in honey bee colonies of differentstrength originated from different Serbian regions... | en |
dc.format | application/pdf | |
dc.language | sr | |
dc.publisher | Универзитет у Београду, Факултет ветеринарске медицине | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS// | |
dc.rights | openAccess | en |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | |
dc.source | Универзитет у Београду | sr |
dc.subject | real-time RT-PCR | sr |
dc.subject | real-time RT-PCR | en |
dc.subject | PCR | en |
dc.subject | viruses | en |
dc.subject | microsporidia | en |
dc.subject | Apis mellifera | en |
dc.subject | PCR | sr |
dc.subject | virusi | sr |
dc.subject | mikrosporidije | sr |
dc.subject | Apis mellifera | sr |
dc.title | Molekularno-genetička detekcija i identifikacija uzročnika mikrosporidijalnih i virusnih infekcija zastupljenih kod pčelinjih društava na teritoriji Srbije | sr |
dc.title | Molecular detection and identification of microsporidia and viruses in honey bee colonies in Serbia | en |
dc.type | doctoralThesis | en |
dc.rights.license | BY-NC-ND | |
dc.identifier.fulltext | http://veterinar.vet.bg.ac.rs/bitstream/id/11332/Disertacija750.pdf | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_nardus_5111 |