Reliability of molecular tests in diagnosing ovine brucellosis caused by Brucella ovis
Аутори
Zelenović, MladenMarinković, Darko
Stević, Nataša
Stanojević, Slavoljub
Aničić, Milan
Milićević, Vesna
Valčić, Olivera
Radojičić, Sonja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Brucella ovis infects sheep and causes a clinical or subclinical disease characterized by
genital lesions and reduced fertility in rams, placentitis and abortions in ewes, and
increased perinatal mortality in lambs. As part of this study, analyses were conducted
on a sheep farm near Belgrade (Serbia). Of the serological tests, indirect ELISA was
performed. A total of 94 blood sera were analyzed, 33 from rams and 61 from ewes.
The results showed 23 (69.7%) positive findings in rams and 2 (3.3%) positive findings
in ewes, with an overall prevalence of 25.4% bounded by a 95% confidence interval.
Bruce-ladder multiplex PCR and Real time PCR were performed on 19 serologically
positive rams and two serologically positive ewes. The results indicate a slightly
higher sensitivity of Real time PCR compared to conventional PCR in diagnosing
B. ovis from the reproductive tissues of rams. However, the differences in sensitivity
between different nucleic acid extraction protocols were not... significant. Most rams
showed a positive PCR result in only one sample of reproductive tissue, suggesting the
recommendation to take multiple samples from each animal. Further research is needed
to bring the sensitivity of molecular tests in diagnosing ram epididymitis closer to the
sensitivity of serological tests.
Кључне речи:
Brucella ovis / ELISA / molecular diagnostics / sheepИзвор:
Acta Veterinaria, 2024, 74, 1, 133-144Издавач:
- Belgrade : Faculty of Veterinary Medicine
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200143 (Универзитет у Београду, Факултет ветеринарске медицине) (RS-MESTD-inst-2020-200143)
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Zelenović, Mladen AU - Marinković, Darko AU - Stević, Nataša AU - Stanojević, Slavoljub AU - Aničić, Milan AU - Milićević, Vesna AU - Valčić, Olivera AU - Radojičić, Sonja PY - 2024 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3790 AB - Brucella ovis infects sheep and causes a clinical or subclinical disease characterized by genital lesions and reduced fertility in rams, placentitis and abortions in ewes, and increased perinatal mortality in lambs. As part of this study, analyses were conducted on a sheep farm near Belgrade (Serbia). Of the serological tests, indirect ELISA was performed. A total of 94 blood sera were analyzed, 33 from rams and 61 from ewes. The results showed 23 (69.7%) positive findings in rams and 2 (3.3%) positive findings in ewes, with an overall prevalence of 25.4% bounded by a 95% confidence interval. Bruce-ladder multiplex PCR and Real time PCR were performed on 19 serologically positive rams and two serologically positive ewes. The results indicate a slightly higher sensitivity of Real time PCR compared to conventional PCR in diagnosing B. ovis from the reproductive tissues of rams. However, the differences in sensitivity between different nucleic acid extraction protocols were not significant. Most rams showed a positive PCR result in only one sample of reproductive tissue, suggesting the recommendation to take multiple samples from each animal. Further research is needed to bring the sensitivity of molecular tests in diagnosing ram epididymitis closer to the sensitivity of serological tests. PB - Belgrade : Faculty of Veterinary Medicine T2 - Acta Veterinaria T1 - Reliability of molecular tests in diagnosing ovine brucellosis caused by Brucella ovis VL - 74 IS - 1 SP - 133 EP - 144 DO - 10.2478/acve-2024-0010 ER -
@article{ author = "Zelenović, Mladen and Marinković, Darko and Stević, Nataša and Stanojević, Slavoljub and Aničić, Milan and Milićević, Vesna and Valčić, Olivera and Radojičić, Sonja", year = "2024", abstract = "Brucella ovis infects sheep and causes a clinical or subclinical disease characterized by genital lesions and reduced fertility in rams, placentitis and abortions in ewes, and increased perinatal mortality in lambs. As part of this study, analyses were conducted on a sheep farm near Belgrade (Serbia). Of the serological tests, indirect ELISA was performed. A total of 94 blood sera were analyzed, 33 from rams and 61 from ewes. The results showed 23 (69.7%) positive findings in rams and 2 (3.3%) positive findings in ewes, with an overall prevalence of 25.4% bounded by a 95% confidence interval. Bruce-ladder multiplex PCR and Real time PCR were performed on 19 serologically positive rams and two serologically positive ewes. The results indicate a slightly higher sensitivity of Real time PCR compared to conventional PCR in diagnosing B. ovis from the reproductive tissues of rams. However, the differences in sensitivity between different nucleic acid extraction protocols were not significant. Most rams showed a positive PCR result in only one sample of reproductive tissue, suggesting the recommendation to take multiple samples from each animal. Further research is needed to bring the sensitivity of molecular tests in diagnosing ram epididymitis closer to the sensitivity of serological tests.", publisher = "Belgrade : Faculty of Veterinary Medicine", journal = "Acta Veterinaria", title = "Reliability of molecular tests in diagnosing ovine brucellosis caused by Brucella ovis", volume = "74", number = "1", pages = "133-144", doi = "10.2478/acve-2024-0010" }
Zelenović, M., Marinković, D., Stević, N., Stanojević, S., Aničić, M., Milićević, V., Valčić, O.,& Radojičić, S.. (2024). Reliability of molecular tests in diagnosing ovine brucellosis caused by Brucella ovis. in Acta Veterinaria Belgrade : Faculty of Veterinary Medicine., 74(1), 133-144. https://doi.org/10.2478/acve-2024-0010
Zelenović M, Marinković D, Stević N, Stanojević S, Aničić M, Milićević V, Valčić O, Radojičić S. Reliability of molecular tests in diagnosing ovine brucellosis caused by Brucella ovis. in Acta Veterinaria. 2024;74(1):133-144. doi:10.2478/acve-2024-0010 .
Zelenović, Mladen, Marinković, Darko, Stević, Nataša, Stanojević, Slavoljub, Aničić, Milan, Milićević, Vesna, Valčić, Olivera, Radojičić, Sonja, "Reliability of molecular tests in diagnosing ovine brucellosis caused by Brucella ovis" in Acta Veterinaria, 74, no. 1 (2024):133-144, https://doi.org/10.2478/acve-2024-0010 . .