Influence of different glucocorticosteroid treatment regimens on pathohistological changes in hearts of rats poisoned with T-2 toxin
Uticaj različitih glukokortikosteroidnih terapijskih režima na patohistološke promene u srcu pacova trovanih T-2 toksinom
2006
Autori
Jaćević, VesnaZolotarevski, Lidija
Milosavljević, I.
Jelić, Katarina
Resanović, Radmila
Bokonjić, Dubravko
Stojiljković, M.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In this study the protective effect of methylprednisolone (soluble form Lemod-solu® and depot form, Lemod-depo®) (40 mg/kg im) on pathohistological changes in hearts of Wistar rats poisoned with T-2 toxin (0.23 mg/kg sc) was examined. Pathohistological, quantitative and morphometric analysis was based on the haematoxylin and eosin (HE) method. Animals were sacrificed after the end of day 1, 3, 5 and 7 of the study. In the hearts of poisoned animals T-2 toxin caused massive, diffuse degenerative and vascular changes associated with gross necrotic areas. The described changes could be found only sporadically in poisoned rats protected with tested methylprednisolone formulation. The best protective effect was produced by the soluble form of methylprednisolone and the least one with a combination of both tested formulations of the drug. The pathohistological alterations in the methylprednisolone protected animals varied from parenchymatous dystrophy to hyaloid degeneration, hyperaemia and ...haemorrhages with mononuclear cell infiltration. These histological deformations of the myocardial architecture were focal. Based on these results, it could be concluded that methylprednisolone formulations, both short and long-acting ones, exert a significant protection of rat hearts from T-2 toxin- induced pathohistological changes.
U okviru ovog rada praćen je uticaj različitih oblika metilprednizolona (Lemod- solu® i Lemod-depo®) apikovanih u dozi od 40 mg/kg im na patohistološke promene u srcu Wistar pacova akutno trovanih citotoksičnim trihotecenskim mikotoksinom, T-2 toksinom (0.23 mg/kg sc). Patohistološka kvantitaivna i morfometrijska analiza histoloških preparata je vršena primenom nakon njihovog bojenja hematoksilin-eozinom (HE). Ispitivane životinje su žrtvovane 1, 3, 5. i 7. dana eksperimenta. U srcu trovanih pacova T-2 toksin je izazvao masivne, difuzne, degenerativne i vaskularne promene koje su bile okružene sa velikim nekrotičnim poljima. Opisane promene bile su najmanje izražene kod trovanih životinja koje su primale preparat Lemod-solu®. Ove fokalne patohistološke promene varirale su od parenhimatozne distrofije do hijaline degeneracije, hiperemije, hemoragija i mononuklearnog ćelijskog infiltrata. Kod malog broja ćelija citoplazma je bila razložena, a njihova jedra su imala normalan izgled ili su... bila blago izdužena ili okrugla. Prisustvo bazofilnog, granuliranog ćelijskog detritusa moglo se uočiti na jednom polu malog broja miofibrila. Veoma je važno naglasiti da su ove promene normalne histološke arhitekture miokarda bile isključivo fokalnog karaktera. Na osnovu prikazanih rezultata može da se zaključi da metilprednizolonske formulacije - i kratko- i dugo delujuća proizvode značajne antidotske efekte kod pacova trovanih T-2 toksinom.
Ključne reči:
trichothecenes / mycotoxins / T-2 toxin / methylprednisolone / pathohistology / heartIzvor:
Acta Veterinaria-Beograd, 2006, 56, 2-3, 243-257Izdavač:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
DOI: 10.2298/AVB0603243J
ISSN: 0567-8315
WoS: 000238491800014
Scopus: 2-s2.0-33745857654
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Jaćević, Vesna AU - Zolotarevski, Lidija AU - Milosavljević, I. AU - Jelić, Katarina AU - Resanović, Radmila AU - Bokonjić, Dubravko AU - Stojiljković, M. PY - 2006 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/382 AB - In this study the protective effect of methylprednisolone (soluble form Lemod-solu® and depot form, Lemod-depo®) (40 mg/kg im) on pathohistological changes in hearts of Wistar rats poisoned with T-2 toxin (0.23 mg/kg sc) was examined. Pathohistological, quantitative and morphometric analysis was based on the haematoxylin and eosin (HE) method. Animals were sacrificed after the end of day 1, 3, 5 and 7 of the study. In the hearts of poisoned animals T-2 toxin caused massive, diffuse degenerative and vascular changes associated with gross necrotic areas. The described changes could be found only sporadically in poisoned rats protected with tested methylprednisolone formulation. The best protective effect was produced by the soluble form of methylprednisolone and the least one with a combination of both tested formulations of the drug. The pathohistological alterations in the methylprednisolone protected animals varied from parenchymatous dystrophy to hyaloid degeneration, hyperaemia and haemorrhages with mononuclear cell infiltration. These histological deformations of the myocardial architecture were focal. Based on these results, it could be concluded that methylprednisolone formulations, both short and long-acting ones, exert a significant protection of rat hearts from T-2 toxin- induced pathohistological changes. AB - U okviru ovog rada praćen je uticaj različitih oblika metilprednizolona (Lemod- solu® i Lemod-depo®) apikovanih u dozi od 40 mg/kg im na patohistološke promene u srcu Wistar pacova akutno trovanih citotoksičnim trihotecenskim mikotoksinom, T-2 toksinom (0.23 mg/kg sc). Patohistološka kvantitaivna i morfometrijska analiza histoloških preparata je vršena primenom nakon njihovog bojenja hematoksilin-eozinom (HE). Ispitivane životinje su žrtvovane 1, 3, 5. i 7. dana eksperimenta. U srcu trovanih pacova T-2 toksin je izazvao masivne, difuzne, degenerativne i vaskularne promene koje su bile okružene sa velikim nekrotičnim poljima. Opisane promene bile su najmanje izražene kod trovanih životinja koje su primale preparat Lemod-solu®. Ove fokalne patohistološke promene varirale su od parenhimatozne distrofije do hijaline degeneracije, hiperemije, hemoragija i mononuklearnog ćelijskog infiltrata. Kod malog broja ćelija citoplazma je bila razložena, a njihova jedra su imala normalan izgled ili su bila blago izdužena ili okrugla. Prisustvo bazofilnog, granuliranog ćelijskog detritusa moglo se uočiti na jednom polu malog broja miofibrila. Veoma je važno naglasiti da su ove promene normalne histološke arhitekture miokarda bile isključivo fokalnog karaktera. Na osnovu prikazanih rezultata može da se zaključi da metilprednizolonske formulacije - i kratko- i dugo delujuća proizvode značajne antidotske efekte kod pacova trovanih T-2 toksinom. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Influence of different glucocorticosteroid treatment regimens on pathohistological changes in hearts of rats poisoned with T-2 toxin T1 - Uticaj različitih glukokortikosteroidnih terapijskih režima na patohistološke promene u srcu pacova trovanih T-2 toksinom VL - 56 IS - 2-3 SP - 243 EP - 257 DO - 10.2298/AVB0603243J ER -
@article{ author = "Jaćević, Vesna and Zolotarevski, Lidija and Milosavljević, I. and Jelić, Katarina and Resanović, Radmila and Bokonjić, Dubravko and Stojiljković, M.", year = "2006", abstract = "In this study the protective effect of methylprednisolone (soluble form Lemod-solu® and depot form, Lemod-depo®) (40 mg/kg im) on pathohistological changes in hearts of Wistar rats poisoned with T-2 toxin (0.23 mg/kg sc) was examined. Pathohistological, quantitative and morphometric analysis was based on the haematoxylin and eosin (HE) method. Animals were sacrificed after the end of day 1, 3, 5 and 7 of the study. In the hearts of poisoned animals T-2 toxin caused massive, diffuse degenerative and vascular changes associated with gross necrotic areas. The described changes could be found only sporadically in poisoned rats protected with tested methylprednisolone formulation. The best protective effect was produced by the soluble form of methylprednisolone and the least one with a combination of both tested formulations of the drug. The pathohistological alterations in the methylprednisolone protected animals varied from parenchymatous dystrophy to hyaloid degeneration, hyperaemia and haemorrhages with mononuclear cell infiltration. These histological deformations of the myocardial architecture were focal. Based on these results, it could be concluded that methylprednisolone formulations, both short and long-acting ones, exert a significant protection of rat hearts from T-2 toxin- induced pathohistological changes., U okviru ovog rada praćen je uticaj različitih oblika metilprednizolona (Lemod- solu® i Lemod-depo®) apikovanih u dozi od 40 mg/kg im na patohistološke promene u srcu Wistar pacova akutno trovanih citotoksičnim trihotecenskim mikotoksinom, T-2 toksinom (0.23 mg/kg sc). Patohistološka kvantitaivna i morfometrijska analiza histoloških preparata je vršena primenom nakon njihovog bojenja hematoksilin-eozinom (HE). Ispitivane životinje su žrtvovane 1, 3, 5. i 7. dana eksperimenta. U srcu trovanih pacova T-2 toksin je izazvao masivne, difuzne, degenerativne i vaskularne promene koje su bile okružene sa velikim nekrotičnim poljima. Opisane promene bile su najmanje izražene kod trovanih životinja koje su primale preparat Lemod-solu®. Ove fokalne patohistološke promene varirale su od parenhimatozne distrofije do hijaline degeneracije, hiperemije, hemoragija i mononuklearnog ćelijskog infiltrata. Kod malog broja ćelija citoplazma je bila razložena, a njihova jedra su imala normalan izgled ili su bila blago izdužena ili okrugla. Prisustvo bazofilnog, granuliranog ćelijskog detritusa moglo se uočiti na jednom polu malog broja miofibrila. Veoma je važno naglasiti da su ove promene normalne histološke arhitekture miokarda bile isključivo fokalnog karaktera. Na osnovu prikazanih rezultata može da se zaključi da metilprednizolonske formulacije - i kratko- i dugo delujuća proizvode značajne antidotske efekte kod pacova trovanih T-2 toksinom.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Influence of different glucocorticosteroid treatment regimens on pathohistological changes in hearts of rats poisoned with T-2 toxin, Uticaj različitih glukokortikosteroidnih terapijskih režima na patohistološke promene u srcu pacova trovanih T-2 toksinom", volume = "56", number = "2-3", pages = "243-257", doi = "10.2298/AVB0603243J" }
Jaćević, V., Zolotarevski, L., Milosavljević, I., Jelić, K., Resanović, R., Bokonjić, D.,& Stojiljković, M.. (2006). Influence of different glucocorticosteroid treatment regimens on pathohistological changes in hearts of rats poisoned with T-2 toxin. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 56(2-3), 243-257. https://doi.org/10.2298/AVB0603243J
Jaćević V, Zolotarevski L, Milosavljević I, Jelić K, Resanović R, Bokonjić D, Stojiljković M. Influence of different glucocorticosteroid treatment regimens on pathohistological changes in hearts of rats poisoned with T-2 toxin. in Acta Veterinaria-Beograd. 2006;56(2-3):243-257. doi:10.2298/AVB0603243J .
Jaćević, Vesna, Zolotarevski, Lidija, Milosavljević, I., Jelić, Katarina, Resanović, Radmila, Bokonjić, Dubravko, Stojiljković, M., "Influence of different glucocorticosteroid treatment regimens on pathohistological changes in hearts of rats poisoned with T-2 toxin" in Acta Veterinaria-Beograd, 56, no. 2-3 (2006):243-257, https://doi.org/10.2298/AVB0603243J . .