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Investigations of hemadsorption characteristics of glycoprotein HN and F antigens of Newcastle Disease virus envelope in vitro
Ispitivanje hemadsorpcionih svojstava glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti in vitro
dc.creator | Nišavić, Jakov | |
dc.creator | Milić, Nenad | |
dc.date.accessioned | 2020-06-03T12:58:33Z | |
dc.date.available | 2020-06-03T12:58:33Z | |
dc.date.issued | 2006 | |
dc.identifier.issn | 0350-2457 | |
dc.identifier.uri | https://vet-erinar.vet.bg.ac.rs/handle/123456789/387 | |
dc.description.abstract | The objective of these investigations was to examine the hemadsorption characteristics of glycoprotein HN and F antigens of the Newcastle Disease virus envelope. Six hours following inoculation of the cell line with the Newcastle Disease Vero virus, previously activated with 0.025 g/dl trypsin-versene, the appearance of hemadsorption was established in a smaller intensity in antigens diluted in ratios from 1:8 to 1:16. Hemadsorption was also determined in the mentioned cell lines 12 hours following inoculation in viruses diluted 1:64, while hemadsorption was present in virus dilutions of 1:256 after 24 hours. Inhibition of hemadsorption in inoculated cells with immune serum dilutions of 1:64 was established 24 hours following inoculation of the cell line with Vero samples of the Newcastle Disease virus, activated with trypsin-versene and the addition of specific immune serum. | en |
dc.description.abstract | Cilj naših istraživanja bilo je ispitivanje hemadsorpcionih svojstava glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti. Posle 6 časova od inokulacije ćelijske linije Vero virusom Newcastle bolesti, prethodno aktivisanim sa 0,025 g/dl tripsinversena, pojava hemadsorpcije je ustanovljena u manjem intenzitetu u razređenjima antigena od 1:8 do 1:16. Hemadsorpcija je ustanovljena u pomenutim ćelijskim linijama i posle 12 časova od inokulacije u razređenju virusa od 1:64, dok je posle 24 časa od inokulacije hemadsorpcija bila prisutna i u razređenju virusa od 1:256. Posle 24 časa od inokulacije ćelijske linije Vero uzorcima virusa Newcastle bolesti, aktivisanih tripsin-versenom i dodavanja specifičnog imunog seruma, ustanovljena je inhibicija hemadsorpcije kod inokulisanih ćelija sa razređenjem imunog seruma od 1:64. | sr |
dc.publisher | Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Veterinarski Glasnik | |
dc.subject | Newcastle Disease virus | en |
dc.subject | Vero cells | en |
dc.subject | hemadsorption | en |
dc.subject | hemadsorption inhibition | en |
dc.subject | Virus Newcastle bolesti | sr |
dc.subject | Vero ćelije | sr |
dc.subject | hemadsorpcija | sr |
dc.subject | inhibicija hemadsorpcije | sr |
dc.title | Investigations of hemadsorption characteristics of glycoprotein HN and F antigens of Newcastle Disease virus envelope in vitro | en |
dc.title | Ispitivanje hemadsorpcionih svojstava glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti in vitro | sr |
dc.type | article | |
dc.rights.license | BY | |
dcterms.abstract | Нишавић, Јаков; Милић, Ненад; | |
dc.citation.volume | 60 | |
dc.citation.issue | 5-6 | |
dc.citation.spage | 299 | |
dc.citation.epage | 304 | |
dc.citation.other | 60(5-6): 299-304 | |
dc.identifier.doi | 10.2298/VETGL0606299N | |
dc.identifier.fulltext | https://vet-erinar.vet.bg.ac.rs/bitstream/id/952/386.pdf | |
dc.type.version | publishedVersion |