Investigations of genotoxic potential of levamisole hydrochloride in bone marrow cells of Wistar rats
Ispitivanje genotoksičnog potencijala levamizol hidrohlorida na ćelijama kostne srži pacova Wistar soja
Apstrakt
An experiment was performed under in vivo conditions on bone marrow cells of Wistar rats. The following doses of levamisole hydrochloride were tested: a therapeutic dose of 2.2 mg/kg bm, a dose of 4.4 mg/kg bm, LD50 -25% mg/kg bm, and LD50 -75% mg/kg bm. We followed the effect of levamisole hydrochloride on kinetics of the cell cycle and the appearance of structural and numeric changes in chromosomes in bone marrow cells. The therapeutic dose of levamisole of 2.2 mg/kg bm exhibited a capability to increase mitotic activity in the observed cells, thus confirming knowledge of the immunostimulative effect of this dose of the medicine under in vivo conditions. The other tested doses of levamisole in this experiment, observed in comparison with the control group, had an opposite effect, namely, they caused a reduction in the mitotic activity of bone marrow cells. All the examined doses in vivo exhibited the ability to induce numeric (aneuploid and polyploid) and structural (lesions, breaks ...and insertions) chromosomal aberrations. It can be concluded on the grounds of these findings that the examined doses have a genotoxic effect.
Eksperiment je izveden u in vivo uslovima na ćelijama kostne srži pacova Wistar soja. Testirane su doze levamizol hidrohlorida i to: terapijska doza od 2.2 mg/kg, t.m., zatim doza od 4,4 mg/kg t.m., LD50 do 25% mg/kg t.m. i LD50 -75% mg/kg t.m.. Praćen je uticaj levamizol hidrohlorida na kinetiku ćelijskog ciklusa i pojavljivanje strukturnih i numeričkih promena na hromozomima u ćelijama kostne srži. Terapijska doza levamizola od 2,2 mg/kg t.m. pokazala je sposobnost povećanja mitotske aktivnosti posmatranih ćelija, tako potkrepljujući saznanja o imunostimulativnom efektu ove doze leka u in vivo uslovima. Ostale testirane doze levamizola u ovom eksperimentu, posmatrano u odnosu na kontrolnu grupu, imale su suprotan efekat, tj. uslovile su smanjenje mitotske aktivnosti ćelija kostne srži. Sve ispitivane doze in vivo pokazuju sposobnost indukcije numeričkih (aneuploidija i polipleidija) i strukturnih (lezije, prekidi i insercije) aberacija hromozoma. Na osnovu toga može da se zaključi da... ispitivane doze imaju genotoksični efekat.
Ključne reči:
genotoxity / cytotoxicity / aneuploid / polyploid lesions / breaks / insertions / genotoksičnost / citotoksičnost / aneuploidije / poliploidijelezije / prekidi / insercijeIzvor:
Veterinarski Glasnik, 2006, 60, 1-2, 3-9Izdavač:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Kulić, Milan AU - Stanimirović, Zoran AU - Marković, Biljana AU - Ristić, Siniša PY - 2006 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/396 AB - An experiment was performed under in vivo conditions on bone marrow cells of Wistar rats. The following doses of levamisole hydrochloride were tested: a therapeutic dose of 2.2 mg/kg bm, a dose of 4.4 mg/kg bm, LD50 -25% mg/kg bm, and LD50 -75% mg/kg bm. We followed the effect of levamisole hydrochloride on kinetics of the cell cycle and the appearance of structural and numeric changes in chromosomes in bone marrow cells. The therapeutic dose of levamisole of 2.2 mg/kg bm exhibited a capability to increase mitotic activity in the observed cells, thus confirming knowledge of the immunostimulative effect of this dose of the medicine under in vivo conditions. The other tested doses of levamisole in this experiment, observed in comparison with the control group, had an opposite effect, namely, they caused a reduction in the mitotic activity of bone marrow cells. All the examined doses in vivo exhibited the ability to induce numeric (aneuploid and polyploid) and structural (lesions, breaks and insertions) chromosomal aberrations. It can be concluded on the grounds of these findings that the examined doses have a genotoxic effect. AB - Eksperiment je izveden u in vivo uslovima na ćelijama kostne srži pacova Wistar soja. Testirane su doze levamizol hidrohlorida i to: terapijska doza od 2.2 mg/kg, t.m., zatim doza od 4,4 mg/kg t.m., LD50 do 25% mg/kg t.m. i LD50 -75% mg/kg t.m.. Praćen je uticaj levamizol hidrohlorida na kinetiku ćelijskog ciklusa i pojavljivanje strukturnih i numeričkih promena na hromozomima u ćelijama kostne srži. Terapijska doza levamizola od 2,2 mg/kg t.m. pokazala je sposobnost povećanja mitotske aktivnosti posmatranih ćelija, tako potkrepljujući saznanja o imunostimulativnom efektu ove doze leka u in vivo uslovima. Ostale testirane doze levamizola u ovom eksperimentu, posmatrano u odnosu na kontrolnu grupu, imale su suprotan efekat, tj. uslovile su smanjenje mitotske aktivnosti ćelija kostne srži. Sve ispitivane doze in vivo pokazuju sposobnost indukcije numeričkih (aneuploidija i polipleidija) i strukturnih (lezije, prekidi i insercije) aberacija hromozoma. Na osnovu toga može da se zaključi da ispitivane doze imaju genotoksični efekat. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Veterinarski Glasnik T1 - Investigations of genotoxic potential of levamisole hydrochloride in bone marrow cells of Wistar rats T1 - Ispitivanje genotoksičnog potencijala levamizol hidrohlorida na ćelijama kostne srži pacova Wistar soja VL - 60 IS - 1-2 SP - 3 EP - 9 DO - 10.2298/VETGL0602003K ER -
@article{ author = "Kulić, Milan and Stanimirović, Zoran and Marković, Biljana and Ristić, Siniša", year = "2006", abstract = "An experiment was performed under in vivo conditions on bone marrow cells of Wistar rats. The following doses of levamisole hydrochloride were tested: a therapeutic dose of 2.2 mg/kg bm, a dose of 4.4 mg/kg bm, LD50 -25% mg/kg bm, and LD50 -75% mg/kg bm. We followed the effect of levamisole hydrochloride on kinetics of the cell cycle and the appearance of structural and numeric changes in chromosomes in bone marrow cells. The therapeutic dose of levamisole of 2.2 mg/kg bm exhibited a capability to increase mitotic activity in the observed cells, thus confirming knowledge of the immunostimulative effect of this dose of the medicine under in vivo conditions. The other tested doses of levamisole in this experiment, observed in comparison with the control group, had an opposite effect, namely, they caused a reduction in the mitotic activity of bone marrow cells. All the examined doses in vivo exhibited the ability to induce numeric (aneuploid and polyploid) and structural (lesions, breaks and insertions) chromosomal aberrations. It can be concluded on the grounds of these findings that the examined doses have a genotoxic effect., Eksperiment je izveden u in vivo uslovima na ćelijama kostne srži pacova Wistar soja. Testirane su doze levamizol hidrohlorida i to: terapijska doza od 2.2 mg/kg, t.m., zatim doza od 4,4 mg/kg t.m., LD50 do 25% mg/kg t.m. i LD50 -75% mg/kg t.m.. Praćen je uticaj levamizol hidrohlorida na kinetiku ćelijskog ciklusa i pojavljivanje strukturnih i numeričkih promena na hromozomima u ćelijama kostne srži. Terapijska doza levamizola od 2,2 mg/kg t.m. pokazala je sposobnost povećanja mitotske aktivnosti posmatranih ćelija, tako potkrepljujući saznanja o imunostimulativnom efektu ove doze leka u in vivo uslovima. Ostale testirane doze levamizola u ovom eksperimentu, posmatrano u odnosu na kontrolnu grupu, imale su suprotan efekat, tj. uslovile su smanjenje mitotske aktivnosti ćelija kostne srži. Sve ispitivane doze in vivo pokazuju sposobnost indukcije numeričkih (aneuploidija i polipleidija) i strukturnih (lezije, prekidi i insercije) aberacija hromozoma. Na osnovu toga može da se zaključi da ispitivane doze imaju genotoksični efekat.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Veterinarski Glasnik", title = "Investigations of genotoxic potential of levamisole hydrochloride in bone marrow cells of Wistar rats, Ispitivanje genotoksičnog potencijala levamizol hidrohlorida na ćelijama kostne srži pacova Wistar soja", volume = "60", number = "1-2", pages = "3-9", doi = "10.2298/VETGL0602003K" }
Kulić, M., Stanimirović, Z., Marković, B.,& Ristić, S.. (2006). Investigations of genotoxic potential of levamisole hydrochloride in bone marrow cells of Wistar rats. in Veterinarski Glasnik Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(1-2), 3-9. https://doi.org/10.2298/VETGL0602003K
Kulić M, Stanimirović Z, Marković B, Ristić S. Investigations of genotoxic potential of levamisole hydrochloride in bone marrow cells of Wistar rats. in Veterinarski Glasnik. 2006;60(1-2):3-9. doi:10.2298/VETGL0602003K .
Kulić, Milan, Stanimirović, Zoran, Marković, Biljana, Ristić, Siniša, "Investigations of genotoxic potential of levamisole hydrochloride in bone marrow cells of Wistar rats" in Veterinarski Glasnik, 60, no. 1-2 (2006):3-9, https://doi.org/10.2298/VETGL0602003K . .