Приказ основних података о документу
Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)
dc.creator | Kovačević-Filipović, Milica | |
dc.creator | Petakov, Marijana | |
dc.creator | Hermitte, Francis | |
dc.creator | Debeissat, Christelle | |
dc.creator | Krstić, Aleksandra | |
dc.creator | Jovčić, Gordana | |
dc.creator | Biligarski, Dijana | |
dc.creator | Lafarge, Xavier | |
dc.creator | Milenković, Pavle | |
dc.creator | Praloran, Vincent | |
dc.creator | Ivanović, Zoran | |
dc.date.accessioned | 2020-06-03T13:03:31Z | |
dc.date.available | 2020-06-03T13:03:31Z | |
dc.date.issued | 2007 | |
dc.identifier.issn | 0021-9541 | |
dc.identifier.uri | https://vet-erinar.vet.bg.ac.rs/handle/123456789/458 | |
dc.description.abstract | Low O-2 concentration (1%) favors the self-renewal of hematopoietic stem cells and inhibits committed progenitors (CFC). Since IL-6 influences both stem cells and committed progenitors at 20% O-2, we studied its effects in cultures at 1% O-2. The pre-CFC activity in Lin- population of mouse bone marrow was analyzed following 10 days of serum-free culture in medium (LCI) supplemented with IL-3 with and without IL-6, at 20 and 1% O-2 and phenotypic differentiation and proliferative history monitored. The IL-6 receptor expression and initiation of VEGF-A synthesis were also investigated. At 20% O-2, the effects of IL-6 on pre-CFC were negligible but effects on CFC were apparent; conversely, at 1% O-2, the IL-6 enhances activity of pre-CFC but not of CFC. Unlike at 20% O-2, at 1% O-2 a subpopulation of cells remained Lin- in spite of extensive proliferation. However, the absolute number of Lin- cells, did not correlate with pre-CFC activity. A relative increase in VEGF transcripts at 1% O-2 in presence of IL-3 alone was enhanced by the addition of IL-6. IL-6 enhanced pre-CFC activity at 1% O-2 and this was correlated to the induction of VEGF. These data reinforce the concept that physiologically low oxygenation of bone marrow is a regulator of stem cell maintenance. Since the 20% O-2 does not exist in tissues in vivo, further studies in vitro at lower O-2 concentrations should revise our knowledge relating to cytokine effects on stem and progenitor cells. | en |
dc.publisher | Wiley-Liss, Hoboken | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/145048/RS// | |
dc.rights | restrictedAccess | |
dc.source | Journal of Cellular Physiology | |
dc.title | Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC) | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Миленковић, Павле; Хермитте, Францис; Ивановић, Зоран; Крстић, Aлександра; Дебеиссат, Цхристелле; Ковачевић-Филиповић, Милица; Пралоран, Винцент; Лафарге, Xавиер; Билигарски, Дијана; Петаков, Маријана; Јовчић, Гордана; | |
dc.citation.volume | 212 | |
dc.citation.issue | 1 | |
dc.citation.spage | 68 | |
dc.citation.epage | 75 | |
dc.citation.other | 212(1): 68-75 | |
dc.citation.rank | M21 | |
dc.identifier.wos | 000246789300008 | |
dc.identifier.doi | 10.1002/jcp.21003 | |
dc.identifier.pmid | 17311290 | |
dc.identifier.scopus | 2-s2.0-34249905641 | |
dc.type.version | publishedVersion |