In Vitro Evaluation of the Clastogenicity of Fumagillin
Abstract
Fumogillin, an antibiotic compound produced by Aspergillus fumigatus, is effective against microsporidia and various Amoeba species, but is also toxic when administered systemically to mammals. Furthermore, a recent in vivo study by Stanimirovic Z et al. [2007]: (Mutat Res 628: 1-10) indicated genotoxic effects of fumagillin. The aim of the present study was to investigate and explain the clostogenic effects of fumagillin (in the form of fumagillin dicyclohexylamine salt) on human peripheral blood lymphocytes in vitro by sister-chromatid exchanges (SCE), chromosome aberrations (CA), and micronucleus (MN) tests. The mitotic index (MI), proliferation index (PI), and nuclear division index (NDI) were calculated to evaluate the cytotoxic potential of fumagillin. Five concentrations of fumagillin (0.34, 0.68, 1.02, 3.07, and 9.20 mu g/ml) were applied to lymphocyte cultures. All the tested concentrations of fumagillin increased the frequency of SCE per cell significantly (P < 0.001 or P < 0....01) compared with the negative control. A significant (P < 0.001) increase in frequency of structural CA was observed at the three highest concentrations in comparison with the negative control. In addition, the three highest test concentrations increased MN formation and decreased MI, PI, and NDI significantly compared with the negative control. The present results indicate that fumagillin is clostogenic and cytotoxic to cultured human lymphocytes. Environ. Mol. Mutagen. 49:594-601, 2008.
Keywords:
fumagillin dicyclohexylamine salt / sister chromatid exchanges / chromosome aberrations / micronucleus assaySource:
Environmental and Molecular Mutagenesis, 2008, 49, 8, 594-601Publisher:
- Wiley, Hoboken
Funding / projects:
- Ecophysiological and genetic investigations of domestic animals and bees for the purpose of increasing reproductive traits and disease resistance (RS-MESTD-MPN2006-2010-143022)
DOI: 10.1002/em.20409
ISSN: 0893-6692
PubMed: 18613037
WoS: 000260276800002
Scopus: 2-s2.0-54249107151
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Stevanović, Jevrosima AU - Stanimirović, Zoran AU - Radaković, Milena AU - Stojić, Velibor PY - 2008 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/545 AB - Fumogillin, an antibiotic compound produced by Aspergillus fumigatus, is effective against microsporidia and various Amoeba species, but is also toxic when administered systemically to mammals. Furthermore, a recent in vivo study by Stanimirovic Z et al. [2007]: (Mutat Res 628: 1-10) indicated genotoxic effects of fumagillin. The aim of the present study was to investigate and explain the clostogenic effects of fumagillin (in the form of fumagillin dicyclohexylamine salt) on human peripheral blood lymphocytes in vitro by sister-chromatid exchanges (SCE), chromosome aberrations (CA), and micronucleus (MN) tests. The mitotic index (MI), proliferation index (PI), and nuclear division index (NDI) were calculated to evaluate the cytotoxic potential of fumagillin. Five concentrations of fumagillin (0.34, 0.68, 1.02, 3.07, and 9.20 mu g/ml) were applied to lymphocyte cultures. All the tested concentrations of fumagillin increased the frequency of SCE per cell significantly (P < 0.001 or P < 0.01) compared with the negative control. A significant (P < 0.001) increase in frequency of structural CA was observed at the three highest concentrations in comparison with the negative control. In addition, the three highest test concentrations increased MN formation and decreased MI, PI, and NDI significantly compared with the negative control. The present results indicate that fumagillin is clostogenic and cytotoxic to cultured human lymphocytes. Environ. Mol. Mutagen. 49:594-601, 2008. PB - Wiley, Hoboken T2 - Environmental and Molecular Mutagenesis T1 - In Vitro Evaluation of the Clastogenicity of Fumagillin VL - 49 IS - 8 SP - 594 EP - 601 DO - 10.1002/em.20409 ER -
@article{ author = "Stevanović, Jevrosima and Stanimirović, Zoran and Radaković, Milena and Stojić, Velibor", year = "2008", abstract = "Fumogillin, an antibiotic compound produced by Aspergillus fumigatus, is effective against microsporidia and various Amoeba species, but is also toxic when administered systemically to mammals. Furthermore, a recent in vivo study by Stanimirovic Z et al. [2007]: (Mutat Res 628: 1-10) indicated genotoxic effects of fumagillin. The aim of the present study was to investigate and explain the clostogenic effects of fumagillin (in the form of fumagillin dicyclohexylamine salt) on human peripheral blood lymphocytes in vitro by sister-chromatid exchanges (SCE), chromosome aberrations (CA), and micronucleus (MN) tests. The mitotic index (MI), proliferation index (PI), and nuclear division index (NDI) were calculated to evaluate the cytotoxic potential of fumagillin. Five concentrations of fumagillin (0.34, 0.68, 1.02, 3.07, and 9.20 mu g/ml) were applied to lymphocyte cultures. All the tested concentrations of fumagillin increased the frequency of SCE per cell significantly (P < 0.001 or P < 0.01) compared with the negative control. A significant (P < 0.001) increase in frequency of structural CA was observed at the three highest concentrations in comparison with the negative control. In addition, the three highest test concentrations increased MN formation and decreased MI, PI, and NDI significantly compared with the negative control. The present results indicate that fumagillin is clostogenic and cytotoxic to cultured human lymphocytes. Environ. Mol. Mutagen. 49:594-601, 2008.", publisher = "Wiley, Hoboken", journal = "Environmental and Molecular Mutagenesis", title = "In Vitro Evaluation of the Clastogenicity of Fumagillin", volume = "49", number = "8", pages = "594-601", doi = "10.1002/em.20409" }
Stevanović, J., Stanimirović, Z., Radaković, M.,& Stojić, V.. (2008). In Vitro Evaluation of the Clastogenicity of Fumagillin. in Environmental and Molecular Mutagenesis Wiley, Hoboken., 49(8), 594-601. https://doi.org/10.1002/em.20409
Stevanović J, Stanimirović Z, Radaković M, Stojić V. In Vitro Evaluation of the Clastogenicity of Fumagillin. in Environmental and Molecular Mutagenesis. 2008;49(8):594-601. doi:10.1002/em.20409 .
Stevanović, Jevrosima, Stanimirović, Zoran, Radaković, Milena, Stojić, Velibor, "In Vitro Evaluation of the Clastogenicity of Fumagillin" in Environmental and Molecular Mutagenesis, 49, no. 8 (2008):594-601, https://doi.org/10.1002/em.20409 . .