Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry
Uporedno ispitivanje osetljivosti i specifičnosti imunoenzimske probe i testa inhibicije hemaglutinacije u serološkoj dijagnostici newcastle bolesti živine
2009
Аутори
Milić, NenadNišavić, Jakov
Radojičić, Marina
Šekler, Marina
Matović, Kazimir
Vidanović, Dejan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Comparative investigations of the sensitivity and specificity of the indirect immunoenzyme probe - iELISA and the hemagglutination inhibition test (HI test) in serological diagnostics of the Newcastle disease in poultry were carried out using samples of blood serum taken from non-vaccinated and vaccinated poultry. A total of 14 samples of blood serum from non-vaccinated poultry were examined using the immunoenzyme probe - iELISA, and nine of these were found to be positive to the presence of specific antigen against the Newcastle disease virus, while two samples were suspect, and no presence of specific antibodies was established in three samples. Examinations of 82 samples of blood serum from vaccinated poultry for the presence of specific antibodies against the Newcastle disease virus established their presence in 80 serum samples, while one sample was suspect and one sample was negative. The values of the titer of specific antibodies in blood serum samples of vaccinated and non-vacc...inated poultry established using the hemagglutination inhibition test (HI test) ranged from 1:2 to 1:32.
Uporedno ispitivanje osetljivosti i specifičnosti indirektne imunoenzimske probe - iELISA i testa inhibicije hemaglutinacije (HI testa) u serološkoj dijagnostici Newcastle bolesti živine vršeno je korišćenjem uzoraka krvnog seruma nevakcinisane i vakcinisane živine. Primenom imunoenzimske probe - iELISA ukupno je ispitano četrnaest uzoraka krvnog seruma nevakcinisane živine, od kojih je devet bilo pozitivno na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, dok su dva uzorka bila sumnjiva, a kod tri uzorka nije ustanovljeno prisustvo specifičnih antitela. Ispitivanjem 82 uzorka krvnog seruma vakcinisane živine na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, utvrđeno je njihovo prisustvo kod 80 uzoraka seruma, dok je jedan uzorak bio sumnjiv, a jedan negativan. Vrednosti titra specifičnih antitela u uzorcima krvnog seruma vakcinisane i nevakcinisane živine ustanovljene primenom testa inhibicije hemaglutinacije (HI testa), kretale su se od 1:2 do 1:32.
Кључне речи:
Newcastle disease virus / immunoenzyme probe - iELISA / hemagglutination inhibition test (HI test) / virus Newcastle bolesti / imunoenzimska proba - iELISA / test inhibicije hemaglutinacije (HI test)Извор:
Veterinarski Glasnik, 2009, 63, 1-2, 37-44Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Milić, Nenad AU - Nišavić, Jakov AU - Radojičić, Marina AU - Šekler, Marina AU - Matović, Kazimir AU - Vidanović, Dejan PY - 2009 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/580 AB - Comparative investigations of the sensitivity and specificity of the indirect immunoenzyme probe - iELISA and the hemagglutination inhibition test (HI test) in serological diagnostics of the Newcastle disease in poultry were carried out using samples of blood serum taken from non-vaccinated and vaccinated poultry. A total of 14 samples of blood serum from non-vaccinated poultry were examined using the immunoenzyme probe - iELISA, and nine of these were found to be positive to the presence of specific antigen against the Newcastle disease virus, while two samples were suspect, and no presence of specific antibodies was established in three samples. Examinations of 82 samples of blood serum from vaccinated poultry for the presence of specific antibodies against the Newcastle disease virus established their presence in 80 serum samples, while one sample was suspect and one sample was negative. The values of the titer of specific antibodies in blood serum samples of vaccinated and non-vaccinated poultry established using the hemagglutination inhibition test (HI test) ranged from 1:2 to 1:32. AB - Uporedno ispitivanje osetljivosti i specifičnosti indirektne imunoenzimske probe - iELISA i testa inhibicije hemaglutinacije (HI testa) u serološkoj dijagnostici Newcastle bolesti živine vršeno je korišćenjem uzoraka krvnog seruma nevakcinisane i vakcinisane živine. Primenom imunoenzimske probe - iELISA ukupno je ispitano četrnaest uzoraka krvnog seruma nevakcinisane živine, od kojih je devet bilo pozitivno na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, dok su dva uzorka bila sumnjiva, a kod tri uzorka nije ustanovljeno prisustvo specifičnih antitela. Ispitivanjem 82 uzorka krvnog seruma vakcinisane živine na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, utvrđeno je njihovo prisustvo kod 80 uzoraka seruma, dok je jedan uzorak bio sumnjiv, a jedan negativan. Vrednosti titra specifičnih antitela u uzorcima krvnog seruma vakcinisane i nevakcinisane živine ustanovljene primenom testa inhibicije hemaglutinacije (HI testa), kretale su se od 1:2 do 1:32. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Veterinarski Glasnik T1 - Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry T1 - Uporedno ispitivanje osetljivosti i specifičnosti imunoenzimske probe i testa inhibicije hemaglutinacije u serološkoj dijagnostici newcastle bolesti živine VL - 63 IS - 1-2 SP - 37 EP - 44 DO - 10.2298/VETGL0902037M ER -
@article{ author = "Milić, Nenad and Nišavić, Jakov and Radojičić, Marina and Šekler, Marina and Matović, Kazimir and Vidanović, Dejan", year = "2009", abstract = "Comparative investigations of the sensitivity and specificity of the indirect immunoenzyme probe - iELISA and the hemagglutination inhibition test (HI test) in serological diagnostics of the Newcastle disease in poultry were carried out using samples of blood serum taken from non-vaccinated and vaccinated poultry. A total of 14 samples of blood serum from non-vaccinated poultry were examined using the immunoenzyme probe - iELISA, and nine of these were found to be positive to the presence of specific antigen against the Newcastle disease virus, while two samples were suspect, and no presence of specific antibodies was established in three samples. Examinations of 82 samples of blood serum from vaccinated poultry for the presence of specific antibodies against the Newcastle disease virus established their presence in 80 serum samples, while one sample was suspect and one sample was negative. The values of the titer of specific antibodies in blood serum samples of vaccinated and non-vaccinated poultry established using the hemagglutination inhibition test (HI test) ranged from 1:2 to 1:32., Uporedno ispitivanje osetljivosti i specifičnosti indirektne imunoenzimske probe - iELISA i testa inhibicije hemaglutinacije (HI testa) u serološkoj dijagnostici Newcastle bolesti živine vršeno je korišćenjem uzoraka krvnog seruma nevakcinisane i vakcinisane živine. Primenom imunoenzimske probe - iELISA ukupno je ispitano četrnaest uzoraka krvnog seruma nevakcinisane živine, od kojih je devet bilo pozitivno na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, dok su dva uzorka bila sumnjiva, a kod tri uzorka nije ustanovljeno prisustvo specifičnih antitela. Ispitivanjem 82 uzorka krvnog seruma vakcinisane živine na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, utvrđeno je njihovo prisustvo kod 80 uzoraka seruma, dok je jedan uzorak bio sumnjiv, a jedan negativan. Vrednosti titra specifičnih antitela u uzorcima krvnog seruma vakcinisane i nevakcinisane živine ustanovljene primenom testa inhibicije hemaglutinacije (HI testa), kretale su se od 1:2 do 1:32.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Veterinarski Glasnik", title = "Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry, Uporedno ispitivanje osetljivosti i specifičnosti imunoenzimske probe i testa inhibicije hemaglutinacije u serološkoj dijagnostici newcastle bolesti živine", volume = "63", number = "1-2", pages = "37-44", doi = "10.2298/VETGL0902037M" }
Milić, N., Nišavić, J., Radojičić, M., Šekler, M., Matović, K.,& Vidanović, D.. (2009). Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry. in Veterinarski Glasnik Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(1-2), 37-44. https://doi.org/10.2298/VETGL0902037M
Milić N, Nišavić J, Radojičić M, Šekler M, Matović K, Vidanović D. Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry. in Veterinarski Glasnik. 2009;63(1-2):37-44. doi:10.2298/VETGL0902037M .
Milić, Nenad, Nišavić, Jakov, Radojičić, Marina, Šekler, Marina, Matović, Kazimir, Vidanović, Dejan, "Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry" in Veterinarski Glasnik, 63, no. 1-2 (2009):37-44, https://doi.org/10.2298/VETGL0902037M . .