Приказ основних података о документу
The application of polymerase chain reaction in detection of bovine herpesvirus 1 in clinical samples
Primena lančane reakcije polimeraze u dokazivanju prisustva goveđeg herpesvirusa 1 u kliničkim uzorcima materijala
dc.creator | Nišavić, Jakov | |
dc.creator | Milić, Nenad | |
dc.creator | Knežević, Aleksandra | |
dc.creator | Jovanović, Tanja | |
dc.date.accessioned | 2020-06-03T13:18:13Z | |
dc.date.available | 2020-06-03T13:18:13Z | |
dc.date.issued | 2010 | |
dc.identifier.issn | 0567-8315 | |
dc.identifier.uri | https://vet-erinar.vet.bg.ac.rs/handle/123456789/662 | |
dc.description.abstract | The objective of this study was the application of polymerase chain reaction in detection of bovine herpesvirus 1 in clinical samples. Twenty samples of bovine nasal swabs were separately inoculated in Vero cells. Laboratory strain TN 41 of bovine herpesvirus 1 served as a control in the experiment. The cytopathic effects were recovered in the cell line previously inoculated with a sample of bovine nasal swab, as well as in cells inoculated with the laboratory strain TN 41 of the bovine herpesvirus 1 after a period of incubation of 24h, 36h and 48h. Identification of the isolated strains of the virus was done by the virus - neutralization test with specific immune sera against BHV 1, with a titre of 1:16. Comparative analysis of DNA fragments of the laboratory strain of BHV1 and isolated strain obtained by polymerase chain reaction with primers for viral gB glycoprotein and thymidine-kinase coding region, confirmed that the isolated strain of the virus belongs to bovine herpesvirus 1 (BHV 1). | en |
dc.description.abstract | Cilj nàših istraživanja je bio primena lančane reakcije polimeraze u dokazivanju prisustva goveđeg herpesvirusa 1 u kliničkim uzorcima materijala. Dvadeset uzoraka nosnih briseva goveda pojedinačno je inokulisano u ćelijsku liniju Vero. Referentni soj TN 41 virusa BHV 1 služio je kao pozitivna kontrola u ogledu. Posle 24h, 36h i 48h od inokulacije, utvrđena je pojava citopatogenog efekta u ćelijskoj liniji inokulisanoj uzorkom poreklom od jednog nosnog brisa goveda. Iste promene su ustanovljene i posle inokulacije referentnog soja TN 41 virusa BHV 1 u ćelijsku liniju Vero. Identifikacija izolovanog soja virusa poreklom iz uzorka nosnog brisa goveda, vršena je primenom testa virus - neutralizacije uz korišćenje specifičnog imunog seruma protiv virusa BHV 1, titra od 1:16. Uporednom analizom DNK fragmenata referentnog soja virusa BHV 1 i izolovanog soja virusa, dobijenih primenom lančane reakcije polimeraze uz korišćenje dva para prajmera koji su amplifikovali gene na molekulu DNK koji kodiraju sintezu glikoproteina B spoljašnjeg omotača virusa BHV 1 i timidin - kinaze, potvrđena je pripadnost izolovanog soja virusa goveđem herpesvirusu 1. | sr |
dc.publisher | Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/20150/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/145047/RS// | |
dc.rights | openAccess | |
dc.source | Acta Veterinaria-Beograd | |
dc.subject | bovine herpesvirus 1 (BHV 1) | en |
dc.subject | Vero cells | en |
dc.subject | virus -neutralization test (VN-test) | en |
dc.subject | virus isolation | en |
dc.subject | polymerase chain reaction(PCR) | en |
dc.title | The application of polymerase chain reaction in detection of bovine herpesvirus 1 in clinical samples | en |
dc.title | Primena lančane reakcije polimeraze u dokazivanju prisustva goveđeg herpesvirusa 1 u kliničkim uzorcima materijala | sr |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Јовановић, Тања; Милић, Ненад; Нишавић, Јаков; Кнежевић, Aлександра; Примена ланчане реакције полимеразе у доказивању присуства говеђег херпесвируса 1 у клиничким узорцима материјала; Примена ланчане реакције полимеразе у доказивању присуства говеђег херпесвируса 1 у клиничким узорцима материјала; | |
dc.citation.volume | 60 | |
dc.citation.issue | 1 | |
dc.citation.spage | 39 | |
dc.citation.epage | 48 | |
dc.citation.other | 60(1): 39-48 | |
dc.citation.rank | M23 | |
dc.identifier.wos | 000276046300005 | |
dc.identifier.doi | 10.2298/AVB1001039N | |
dc.identifier.scopus | 2-s2.0-77950650435 | |
dc.identifier.fulltext | https://vet-erinar.vet.bg.ac.rs/bitstream/id/1215/661.pdf | |
dc.type.version | publishedVersion |