Treatment for production of interferon-alpha (ifn-α) and interferon-gamma (ifn-γ) from the same purified suspension of leukocytes
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2011
Authors
Stanković, B.Surbatović, M.
Stojanović, G.
Mihajlović, M.
Tambur, Zoran
Kulišić, Zoran
Marković, M.
Article (Published version)
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The basic aim of this work was to examine possibilities for introducing a new biotechnological treatment for preparation, storage and further purifying the suspension of leukocytes ("buffy coat" (BC)), intended for production of human interferon-alpha (hIFN-hIFN-α) and human interferon-gamma (hIFN-γ). Three hundred and eighty four units of BC were examined. They were divided into three series of 128 BC units each - one control series (KSBC) and two experimental series to which 10% dextran solution (ESBC-I) or 6% hydroxyethyl starch solution (ESBC-II) were added. The lowest titer (297929 IU) of hIFN-α ensued from KSBC, a higher titer from ESBC-I (317184 IU) and the highest titer from ESBC-II (364406 IU). We also found that the lowest hIFN- γ titer was produced from KSBC (48142 IU), a somewhat larger one from ESBC-I (67571 IU), and the highest titer was obtained from ESBC-II (73428. IU). This study has demonstrated the positive influence of modified biotechnological treatments on the pro...duction of hIFN-α and hIFN- γ from BC.
Keywords:
Biotechnological treatment / Buffy coat / Interferon-alpha / Interferon-gammaSource:
Scientific Research and Essays, 2011, 6, 7, 1522-1529Collections
Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Stanković, B. AU - Surbatović, M. AU - Stojanović, G. AU - Mihajlović, M. AU - Tambur, Zoran AU - Kulišić, Zoran AU - Marković, M. PY - 2011 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/826 AB - The basic aim of this work was to examine possibilities for introducing a new biotechnological treatment for preparation, storage and further purifying the suspension of leukocytes ("buffy coat" (BC)), intended for production of human interferon-alpha (hIFN-hIFN-α) and human interferon-gamma (hIFN-γ). Three hundred and eighty four units of BC were examined. They were divided into three series of 128 BC units each - one control series (KSBC) and two experimental series to which 10% dextran solution (ESBC-I) or 6% hydroxyethyl starch solution (ESBC-II) were added. The lowest titer (297929 IU) of hIFN-α ensued from KSBC, a higher titer from ESBC-I (317184 IU) and the highest titer from ESBC-II (364406 IU). We also found that the lowest hIFN- γ titer was produced from KSBC (48142 IU), a somewhat larger one from ESBC-I (67571 IU), and the highest titer was obtained from ESBC-II (73428. IU). This study has demonstrated the positive influence of modified biotechnological treatments on the production of hIFN-α and hIFN- γ from BC. T2 - Scientific Research and Essays T1 - Treatment for production of interferon-alpha (ifn-α) and interferon-gamma (ifn-γ) from the same purified suspension of leukocytes VL - 6 IS - 7 SP - 1522 EP - 1529 UR - https://hdl.handle.net/21.15107/rcub_veterinar_826 ER -
@article{ author = "Stanković, B. and Surbatović, M. and Stojanović, G. and Mihajlović, M. and Tambur, Zoran and Kulišić, Zoran and Marković, M.", year = "2011", abstract = "The basic aim of this work was to examine possibilities for introducing a new biotechnological treatment for preparation, storage and further purifying the suspension of leukocytes ("buffy coat" (BC)), intended for production of human interferon-alpha (hIFN-hIFN-α) and human interferon-gamma (hIFN-γ). Three hundred and eighty four units of BC were examined. They were divided into three series of 128 BC units each - one control series (KSBC) and two experimental series to which 10% dextran solution (ESBC-I) or 6% hydroxyethyl starch solution (ESBC-II) were added. The lowest titer (297929 IU) of hIFN-α ensued from KSBC, a higher titer from ESBC-I (317184 IU) and the highest titer from ESBC-II (364406 IU). We also found that the lowest hIFN- γ titer was produced from KSBC (48142 IU), a somewhat larger one from ESBC-I (67571 IU), and the highest titer was obtained from ESBC-II (73428. IU). This study has demonstrated the positive influence of modified biotechnological treatments on the production of hIFN-α and hIFN- γ from BC.", journal = "Scientific Research and Essays", title = "Treatment for production of interferon-alpha (ifn-α) and interferon-gamma (ifn-γ) from the same purified suspension of leukocytes", volume = "6", number = "7", pages = "1522-1529", url = "https://hdl.handle.net/21.15107/rcub_veterinar_826" }
Stanković, B., Surbatović, M., Stojanović, G., Mihajlović, M., Tambur, Z., Kulišić, Z.,& Marković, M.. (2011). Treatment for production of interferon-alpha (ifn-α) and interferon-gamma (ifn-γ) from the same purified suspension of leukocytes. in Scientific Research and Essays, 6(7), 1522-1529. https://hdl.handle.net/21.15107/rcub_veterinar_826
Stanković B, Surbatović M, Stojanović G, Mihajlović M, Tambur Z, Kulišić Z, Marković M. Treatment for production of interferon-alpha (ifn-α) and interferon-gamma (ifn-γ) from the same purified suspension of leukocytes. in Scientific Research and Essays. 2011;6(7):1522-1529. https://hdl.handle.net/21.15107/rcub_veterinar_826 .
Stanković, B., Surbatović, M., Stojanović, G., Mihajlović, M., Tambur, Zoran, Kulišić, Zoran, Marković, M., "Treatment for production of interferon-alpha (ifn-α) and interferon-gamma (ifn-γ) from the same purified suspension of leukocytes" in Scientific Research and Essays, 6, no. 7 (2011):1522-1529, https://hdl.handle.net/21.15107/rcub_veterinar_826 .