Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia
Samo za registrovane korisnike
2012
Autori
Toplak, IvanLazić, Sava
Lupulović, Diana
Prodanov-Radulović, Jasna
Becskei, Zsolt
Došen, Radoslav
Petrović, Tamaš
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Recent variants of porcine circovirus type 2 (PCV2) were obtained from tissues of domestic pigs with porcine circovirus associated disease and from randomly selected wild boar samples from Serbia and Slovenia. A 450-base-pair nucleotide sequence was obtained by PCR from the ORF2. The derived nucleotide and amino acid sequences were aligned and compared to the corresponding region of closely related PCV2 sequences determined in previous years and retrieved from the GenBank. The 30 Serbian and 17 Slovenian PCV2 sequences clustered into three previously determined genotypes (PCV2a: 7), (PCV2b: 38) and (PCV2d: 2). Three major variable regions, concerning 29 amino acid position substitutions within the ORF2, were observed, which further supports the segregation of the detected strains into three separate genotypes. This study indicates that PCV2b is the predominant genotype in Serbia and Slovenia and the detected PCV2 strains are closely related to those previously described in Europe and i...n other parts of the world.
Ključne reči:
Porcine circovirus type 2 / genotype / epidemiology / phylogenetic analysisIzvor:
Acta Veterinaria Hungarica, 2012, 60, 3, 409-420Izdavač:
- Akademiai Kiado Zrt, Budapest
Finansiranje / projekti:
- Praćenje zdravstvenog stanja divljači i uvođenje novih biotehnoloških postupaka u detekciji zaraznih i zoonoznih agenasa - analiza rizika za zdravlje ljudi, domaćih i divljih životinja i kontaminaciju životne sredine (RS-MESTD-Technological Development (TD or TR)-31084)
- Slovenian-Serbian bilateral project
- Slovenian Ministry for Higher Education, Science and Technology [P4 0092]
DOI: 10.1556/AVet.2012.035
ISSN: 0236-6290
PubMed: 22903085
WoS: 000307835100011
Scopus: 2-s2.0-84982272474
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Toplak, Ivan AU - Lazić, Sava AU - Lupulović, Diana AU - Prodanov-Radulović, Jasna AU - Becskei, Zsolt AU - Došen, Radoslav AU - Petrović, Tamaš PY - 2012 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/936 AB - Recent variants of porcine circovirus type 2 (PCV2) were obtained from tissues of domestic pigs with porcine circovirus associated disease and from randomly selected wild boar samples from Serbia and Slovenia. A 450-base-pair nucleotide sequence was obtained by PCR from the ORF2. The derived nucleotide and amino acid sequences were aligned and compared to the corresponding region of closely related PCV2 sequences determined in previous years and retrieved from the GenBank. The 30 Serbian and 17 Slovenian PCV2 sequences clustered into three previously determined genotypes (PCV2a: 7), (PCV2b: 38) and (PCV2d: 2). Three major variable regions, concerning 29 amino acid position substitutions within the ORF2, were observed, which further supports the segregation of the detected strains into three separate genotypes. This study indicates that PCV2b is the predominant genotype in Serbia and Slovenia and the detected PCV2 strains are closely related to those previously described in Europe and in other parts of the world. PB - Akademiai Kiado Zrt, Budapest T2 - Acta Veterinaria Hungarica T1 - Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia VL - 60 IS - 3 SP - 409 EP - 420 DO - 10.1556/AVet.2012.035 ER -
@article{ author = "Toplak, Ivan and Lazić, Sava and Lupulović, Diana and Prodanov-Radulović, Jasna and Becskei, Zsolt and Došen, Radoslav and Petrović, Tamaš", year = "2012", abstract = "Recent variants of porcine circovirus type 2 (PCV2) were obtained from tissues of domestic pigs with porcine circovirus associated disease and from randomly selected wild boar samples from Serbia and Slovenia. A 450-base-pair nucleotide sequence was obtained by PCR from the ORF2. The derived nucleotide and amino acid sequences were aligned and compared to the corresponding region of closely related PCV2 sequences determined in previous years and retrieved from the GenBank. The 30 Serbian and 17 Slovenian PCV2 sequences clustered into three previously determined genotypes (PCV2a: 7), (PCV2b: 38) and (PCV2d: 2). Three major variable regions, concerning 29 amino acid position substitutions within the ORF2, were observed, which further supports the segregation of the detected strains into three separate genotypes. This study indicates that PCV2b is the predominant genotype in Serbia and Slovenia and the detected PCV2 strains are closely related to those previously described in Europe and in other parts of the world.", publisher = "Akademiai Kiado Zrt, Budapest", journal = "Acta Veterinaria Hungarica", title = "Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia", volume = "60", number = "3", pages = "409-420", doi = "10.1556/AVet.2012.035" }
Toplak, I., Lazić, S., Lupulović, D., Prodanov-Radulović, J., Becskei, Z., Došen, R.,& Petrović, T.. (2012). Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia. in Acta Veterinaria Hungarica Akademiai Kiado Zrt, Budapest., 60(3), 409-420. https://doi.org/10.1556/AVet.2012.035
Toplak I, Lazić S, Lupulović D, Prodanov-Radulović J, Becskei Z, Došen R, Petrović T. Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia. in Acta Veterinaria Hungarica. 2012;60(3):409-420. doi:10.1556/AVet.2012.035 .
Toplak, Ivan, Lazić, Sava, Lupulović, Diana, Prodanov-Radulović, Jasna, Becskei, Zsolt, Došen, Radoslav, Petrović, Tamaš, "Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia" in Acta Veterinaria Hungarica, 60, no. 3 (2012):409-420, https://doi.org/10.1556/AVet.2012.035 . .