Vuković, Dejan

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  • Vuković, Dejan (5)
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Mikroorganizmi kvara hrane

Savić Radovanović, Radoslava; Vuković, Dejan; Kureljušić, Jasna; Rajić-Savić, Nataša

(Ветеринарска комора Републике Српске, 2020)

TY  - CONF
AU  - Savić Radovanović, Radoslava
AU  - Vuković, Dejan
AU  - Kureljušić, Jasna
AU  - Rajić-Savić, Nataša
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2325
AB  - Производња хране представља значајну привредну делатност и улогу у
снабдевању становништва животним намирницама, док квар хране има за
последицу велике економске губитке услед одбацивања хране као
неупотребљиве за исхрану и трошкова уклањања органског отпада. Према
новијим истраживањима 30% произведене хране заврши на светским
депонијама, где подлеже трулежним процесима и распадању, при чему се
ослобађају гасови, међу којима и метан. Према дефиницији квар хране
представља различите процесе, који узрокују да храна буде непожељна или
неприхватљива за људску употребу због промене сензорних карактеристика.
Квар хране се може дефинисати и као "свака сензорна промена (тактилна,
визуелна, олфактивна или промена укуса)" коју конзумент сматра да је
неприхватљива. Квар може настати у било којој фази ланца хране. Све врсте
квара се могу сврстати у следеће групе: биохемијски (ензимски),
микробиолошки, хемијски и технолошки квар. Микробиолошки квар хране
може бити проузрокован узрочницима као што су бактерије, квасци и плесни.
Хемијски квар подразумева промене проузроковане хемијским и физичко-
хемијским реакцијама који настају услед штетног утицаја фактора спољне
средине (високе температуре, ваздух, влага, светлост, pH итд). Често је тешко
утврдити да ли се ради о искључиво хемијском, биохемијском или
микробиолошком квару, јер се промене одвијају паралелно. Под појмом
„технолошки квар“ подразумева се у ширем смислу квар проузрокован
грешкама током технолошког процеса добијања хране. Према подацима
истраживања, које је спровео Центар за унапређење животне средине
2019.године, у Републици Србији је одбачено 247.000 t хране, што на дневном
нивоу износи 676 t, и може се прерачунати да један становник у току године
дневно баци 35 kg хране. Од хране се највише баца хлеб (10,18 kg), месо (7,18
kg) и млеко (6,74 l), а знатно мање храна биљног порекла - воће (5,7 kg ) и
поврће (5,33 kg). Kao разлог због којег се храна баца на првом месту се наводи
квар (67%), затим одбијање конзумената да једу храну, која није свежа (17%) и
мишљење потрошача да храна није безбедна (11%). Један од највећих изазоваза савремену индустрију хране јесте како сачувати намирнице од квара. Због
свог хемијског састава храна, посебно храна животињског порекла (млеко и
производи од млека; месо и производи од меса) представља добру средину за
раст и размножавање микроорганизама, стога је за циљ овог рада постављено
да се укаже на значај микроорганизама, који могу да доведу до квара хране. У
раду ће бити описане врсте микроорганизама, њихове карактеристике и
промене које доводе у храни животињског порекла.
AB  - Food production represents a significant economic activity and has a role in
supplying the population with food, while food spoilage results in large economic
losses due to the rejection of food as unusable and the costs of disposing of organic
waste. According to recent study, 30% of produced food ends up in the world's
landfills, where undergoes putrefaction and decomposition, releasing gases,
including methane. Food spoilage is defined as various processes, which cause
food to be undesirable or unacceptable for human use due to a changes in sensory
characteristics. Food spolage can also be defined as "any sensory change (tactile,
visual, olfactory or aroma change)" that the consumer considers unacceptable.
Spoilage can occur at any stage along food chain. All types of spoilage can be
classified into the following groups: biochemical (enzymatic), microbiological,
chemical and technological spoilage. Microbiological spoilage of food can be
caused by pathogens such as bacteria, yeasts and molds. Chemical spoilage means
changes caused by chemical and physico-chemical reactions that occur due to the
harmful influence of environmental factors (high temperatures, atmosphere,
humidity, light, pH, etc.). It is often difficult to determine whether it is a strict
chemical, biochemical or microbiological spoilage, because the changes take place
in parallel. The term "technological spoilage" means in a broader sense a spoilage
caused by mistakes during the technological process in food production.
According to the research conducted by the Center for Environmental
Improvement in 2019 in the Republic of Serbia, 247,000 tons of food were
discarded, of which is 676 tons on a daily basis, and it can be calculated that one
inhabitant throws 35 kg of food per year. Bread (10.18 kg), meat (7.18 kg) and milk
(6.74 l) are the most discarded food, while much less food of plant origin - fruits
(5.7 kg) and vegetables (5.33 kg). The reason for discarding the food in the first
place is the spoilage (67%), then the refusal of consumers to eat the food, which is
not fresh (17%) and the opinion of consumers that the food is not safe (11%). One
of the biggest challenges for the modern food industry is how to save food from
spoilage. Due to its chemical composition, food, especially food of animal origin (milk and dairy products; meat and meat products) is a good environment for the
growth and muliplication of microorganisms, so the aim of this paper is to point
out the importance of microorganisms that can cause food spoilage. The paper will
describe the types of microorganisms, their characteristics and the changes they
cause in food of animal origin.
PB  - Ветеринарска комора Републике Српске
C3  - 25. Godišnje Savetovanje doktora veterinarske medicine Republike Srpske
T1  - Mikroorganizmi kvara hrane
T1  - Spoilage microorganisms in food
SP  - 110
EP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2325
ER  - 
@conference{
author = "Savić Radovanović, Radoslava and Vuković, Dejan and Kureljušić, Jasna and Rajić-Savić, Nataša",
year = "2020",
abstract = "Производња хране представља значајну привредну делатност и улогу у
снабдевању становништва животним намирницама, док квар хране има за
последицу велике економске губитке услед одбацивања хране као
неупотребљиве за исхрану и трошкова уклањања органског отпада. Према
новијим истраживањима 30% произведене хране заврши на светским
депонијама, где подлеже трулежним процесима и распадању, при чему се
ослобађају гасови, међу којима и метан. Према дефиницији квар хране
представља различите процесе, који узрокују да храна буде непожељна или
неприхватљива за људску употребу због промене сензорних карактеристика.
Квар хране се може дефинисати и као "свака сензорна промена (тактилна,
визуелна, олфактивна или промена укуса)" коју конзумент сматра да је
неприхватљива. Квар може настати у било којој фази ланца хране. Све врсте
квара се могу сврстати у следеће групе: биохемијски (ензимски),
микробиолошки, хемијски и технолошки квар. Микробиолошки квар хране
може бити проузрокован узрочницима као што су бактерије, квасци и плесни.
Хемијски квар подразумева промене проузроковане хемијским и физичко-
хемијским реакцијама који настају услед штетног утицаја фактора спољне
средине (високе температуре, ваздух, влага, светлост, pH итд). Често је тешко
утврдити да ли се ради о искључиво хемијском, биохемијском или
микробиолошком квару, јер се промене одвијају паралелно. Под појмом
„технолошки квар“ подразумева се у ширем смислу квар проузрокован
грешкама током технолошког процеса добијања хране. Према подацима
истраживања, које је спровео Центар за унапређење животне средине
2019.године, у Републици Србији је одбачено 247.000 t хране, што на дневном
нивоу износи 676 t, и може се прерачунати да један становник у току године
дневно баци 35 kg хране. Од хране се највише баца хлеб (10,18 kg), месо (7,18
kg) и млеко (6,74 l), а знатно мање храна биљног порекла - воће (5,7 kg ) и
поврће (5,33 kg). Kao разлог због којег се храна баца на првом месту се наводи
квар (67%), затим одбијање конзумената да једу храну, која није свежа (17%) и
мишљење потрошача да храна није безбедна (11%). Један од највећих изазоваза савремену индустрију хране јесте како сачувати намирнице од квара. Због
свог хемијског састава храна, посебно храна животињског порекла (млеко и
производи од млека; месо и производи од меса) представља добру средину за
раст и размножавање микроорганизама, стога је за циљ овог рада постављено
да се укаже на значај микроорганизама, који могу да доведу до квара хране. У
раду ће бити описане врсте микроорганизама, њихове карактеристике и
промене које доводе у храни животињског порекла., Food production represents a significant economic activity and has a role in
supplying the population with food, while food spoilage results in large economic
losses due to the rejection of food as unusable and the costs of disposing of organic
waste. According to recent study, 30% of produced food ends up in the world's
landfills, where undergoes putrefaction and decomposition, releasing gases,
including methane. Food spoilage is defined as various processes, which cause
food to be undesirable or unacceptable for human use due to a changes in sensory
characteristics. Food spolage can also be defined as "any sensory change (tactile,
visual, olfactory or aroma change)" that the consumer considers unacceptable.
Spoilage can occur at any stage along food chain. All types of spoilage can be
classified into the following groups: biochemical (enzymatic), microbiological,
chemical and technological spoilage. Microbiological spoilage of food can be
caused by pathogens such as bacteria, yeasts and molds. Chemical spoilage means
changes caused by chemical and physico-chemical reactions that occur due to the
harmful influence of environmental factors (high temperatures, atmosphere,
humidity, light, pH, etc.). It is often difficult to determine whether it is a strict
chemical, biochemical or microbiological spoilage, because the changes take place
in parallel. The term "technological spoilage" means in a broader sense a spoilage
caused by mistakes during the technological process in food production.
According to the research conducted by the Center for Environmental
Improvement in 2019 in the Republic of Serbia, 247,000 tons of food were
discarded, of which is 676 tons on a daily basis, and it can be calculated that one
inhabitant throws 35 kg of food per year. Bread (10.18 kg), meat (7.18 kg) and milk
(6.74 l) are the most discarded food, while much less food of plant origin - fruits
(5.7 kg) and vegetables (5.33 kg). The reason for discarding the food in the first
place is the spoilage (67%), then the refusal of consumers to eat the food, which is
not fresh (17%) and the opinion of consumers that the food is not safe (11%). One
of the biggest challenges for the modern food industry is how to save food from
spoilage. Due to its chemical composition, food, especially food of animal origin (milk and dairy products; meat and meat products) is a good environment for the
growth and muliplication of microorganisms, so the aim of this paper is to point
out the importance of microorganisms that can cause food spoilage. The paper will
describe the types of microorganisms, their characteristics and the changes they
cause in food of animal origin.",
publisher = "Ветеринарска комора Републике Српске",
journal = "25. Godišnje Savetovanje doktora veterinarske medicine Republike Srpske",
title = "Mikroorganizmi kvara hrane, Spoilage microorganisms in food",
pages = "110-113",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2325"
}
Savić Radovanović, R., Vuković, D., Kureljušić, J.,& Rajić-Savić, N.. (2020). Mikroorganizmi kvara hrane. in 25. Godišnje Savetovanje doktora veterinarske medicine Republike Srpske
Ветеринарска комора Републике Српске., 110-113.
https://hdl.handle.net/21.15107/rcub_veterinar_2325
Savić Radovanović R, Vuković D, Kureljušić J, Rajić-Savić N. Mikroorganizmi kvara hrane. in 25. Godišnje Savetovanje doktora veterinarske medicine Republike Srpske. 2020;:110-113.
https://hdl.handle.net/21.15107/rcub_veterinar_2325 .
Savić Radovanović, Radoslava, Vuković, Dejan, Kureljušić, Jasna, Rajić-Savić, Nataša, "Mikroorganizmi kvara hrane" in 25. Godišnje Savetovanje doktora veterinarske medicine Republike Srpske (2020):110-113,
https://hdl.handle.net/21.15107/rcub_veterinar_2325 .

The correlation between selected computer assisted sperm analysis parameters and bull fertility

Cojkić, Aleksandar; Dimitrijević, Vladimir; Savić, Mila; Jeremić, Ivan; Vuković, Dejan; Čobanović, Nikola; Obradović, Saša; Petrujkić, Branko

(Univ Zagreb Vet Faculty, Zagreb, 2017)

TY  - JOUR
AU  - Cojkić, Aleksandar
AU  - Dimitrijević, Vladimir
AU  - Savić, Mila
AU  - Jeremić, Ivan
AU  - Vuković, Dejan
AU  - Čobanović, Nikola
AU  - Obradović, Saša
AU  - Petrujkić, Branko
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1489
AB  - Computer Assisted Sperm Analysis (CASA) represents an objective, reproducible and reliable method of sperm quality assessment, however, not many reports exist that correlate its accuracy with bull semen fertility. The aim of this study was to evaluate the correlation between selected CASA motility parameters of cryopreserved bull semen and fertility. The total spermatozoa motility (SM %), the progressive spermatozoa motility (PSM %) as well as the percentage of spermatozoa with rapid movement (RAP %) were measured through CASA. All 12 ejaculates were collected from one Holstein Friesian bull. A total of 816 Holstein Friesian cows were used for artificial insemination (AI) and the evaluation of fertility. The fertility success was assessed by pregnancy rates per cycle (PRC %), 90 days after AI of the cows. The sperm variables that were associated with an increase in the PRC were the SM (R2 = 0. 6722), the PSM (R2 = 0. 6520) and the RAP (R2 = 0. 7103). RAP had a greater influence (P<0.001) on fertility (PRC), than SM and PSM (P<0.01). The increase of sperm motility parameters (SM, PSM and RAP) led to increased PRC, i.e. to increased fertility.
PB  - Univ Zagreb Vet Faculty, Zagreb
T2  - Veterinarski Arhiv
T1  - The correlation between selected computer assisted sperm analysis parameters and bull fertility
T1  - Odnos između odabranih pokazatelja kompjutorski potpomognute analize sperme i plodnosti bikova
VL  - 87
IS  - 2
SP  - 129
EP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1489
ER  - 
@article{
author = "Cojkić, Aleksandar and Dimitrijević, Vladimir and Savić, Mila and Jeremić, Ivan and Vuković, Dejan and Čobanović, Nikola and Obradović, Saša and Petrujkić, Branko",
year = "2017",
abstract = "Computer Assisted Sperm Analysis (CASA) represents an objective, reproducible and reliable method of sperm quality assessment, however, not many reports exist that correlate its accuracy with bull semen fertility. The aim of this study was to evaluate the correlation between selected CASA motility parameters of cryopreserved bull semen and fertility. The total spermatozoa motility (SM %), the progressive spermatozoa motility (PSM %) as well as the percentage of spermatozoa with rapid movement (RAP %) were measured through CASA. All 12 ejaculates were collected from one Holstein Friesian bull. A total of 816 Holstein Friesian cows were used for artificial insemination (AI) and the evaluation of fertility. The fertility success was assessed by pregnancy rates per cycle (PRC %), 90 days after AI of the cows. The sperm variables that were associated with an increase in the PRC were the SM (R2 = 0. 6722), the PSM (R2 = 0. 6520) and the RAP (R2 = 0. 7103). RAP had a greater influence (P<0.001) on fertility (PRC), than SM and PSM (P<0.01). The increase of sperm motility parameters (SM, PSM and RAP) led to increased PRC, i.e. to increased fertility.",
publisher = "Univ Zagreb Vet Faculty, Zagreb",
journal = "Veterinarski Arhiv",
title = "The correlation between selected computer assisted sperm analysis parameters and bull fertility, Odnos između odabranih pokazatelja kompjutorski potpomognute analize sperme i plodnosti bikova",
volume = "87",
number = "2",
pages = "129-137",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1489"
}
Cojkić, A., Dimitrijević, V., Savić, M., Jeremić, I., Vuković, D., Čobanović, N., Obradović, S.,& Petrujkić, B.. (2017). The correlation between selected computer assisted sperm analysis parameters and bull fertility. in Veterinarski Arhiv
Univ Zagreb Vet Faculty, Zagreb., 87(2), 129-137.
https://hdl.handle.net/21.15107/rcub_veterinar_1489
Cojkić A, Dimitrijević V, Savić M, Jeremić I, Vuković D, Čobanović N, Obradović S, Petrujkić B. The correlation between selected computer assisted sperm analysis parameters and bull fertility. in Veterinarski Arhiv. 2017;87(2):129-137.
https://hdl.handle.net/21.15107/rcub_veterinar_1489 .
Cojkić, Aleksandar, Dimitrijević, Vladimir, Savić, Mila, Jeremić, Ivan, Vuković, Dejan, Čobanović, Nikola, Obradović, Saša, Petrujkić, Branko, "The correlation between selected computer assisted sperm analysis parameters and bull fertility" in Veterinarski Arhiv, 87, no. 2 (2017):129-137,
https://hdl.handle.net/21.15107/rcub_veterinar_1489 .
4
7

Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation

Buac, Marijana; Mojsilović, Slavko; Mišić, Dušan; Vuković, Dejan; Savić, Olivera; Valčić, Olivera; Marković, Dragana; Gvozdić, Dragan; Ilić, Vesna; Fratrić, Natalija

(Elsevier Sci Ltd, Oxford, 2016)

TY  - JOUR
AU  - Buac, Marijana
AU  - Mojsilović, Slavko
AU  - Mišić, Dušan
AU  - Vuković, Dejan
AU  - Savić, Olivera
AU  - Valčić, Olivera
AU  - Marković, Dragana
AU  - Gvozdić, Dragan
AU  - Ilić, Vesna
AU  - Fratrić, Natalija
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1403
AB  - In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes viability. The strongest effect of inhibition of cows granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation
VL  - 106
SP  - 135
EP  - 142
DO  - 10.1016/j.rvsc.2016.04.002
ER  - 
@article{
author = "Buac, Marijana and Mojsilović, Slavko and Mišić, Dušan and Vuković, Dejan and Savić, Olivera and Valčić, Olivera and Marković, Dragana and Gvozdić, Dragan and Ilić, Vesna and Fratrić, Natalija",
year = "2016",
abstract = "In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes viability. The strongest effect of inhibition of cows granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation",
volume = "106",
pages = "135-142",
doi = "10.1016/j.rvsc.2016.04.002"
}
Buac, M., Mojsilović, S., Mišić, D., Vuković, D., Savić, O., Valčić, O., Marković, D., Gvozdić, D., Ilić, V.,& Fratrić, N.. (2016). Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 106, 135-142.
https://doi.org/10.1016/j.rvsc.2016.04.002
Buac M, Mojsilović S, Mišić D, Vuković D, Savić O, Valčić O, Marković D, Gvozdić D, Ilić V, Fratrić N. Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. in Research in Veterinary Science. 2016;106:135-142.
doi:10.1016/j.rvsc.2016.04.002 .
Buac, Marijana, Mojsilović, Slavko, Mišić, Dušan, Vuković, Dejan, Savić, Olivera, Valčić, Olivera, Marković, Dragana, Gvozdić, Dragan, Ilić, Vesna, Fratrić, Natalija, "Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation" in Research in Veterinary Science, 106 (2016):135-142,
https://doi.org/10.1016/j.rvsc.2016.04.002 . .
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Progesterone concentration in milk and blood serum and reproductive efficiency of cows after Ovsynch treatment

Vuković, Dejan; Božić, Aleksandar; Relić, Renata; Stančić, Blagoje; Gvozdić, Dragan; Kucević, Denis

(Scientific Technical Research Council Turkey-Tubitak, Ankara, 2016)

TY  - JOUR
AU  - Vuković, Dejan
AU  - Božić, Aleksandar
AU  - Relić, Renata
AU  - Stančić, Blagoje
AU  - Gvozdić, Dragan
AU  - Kucević, Denis
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1413
AB  - An experiment was conducted to investigate the effects of hormonal synchronization of ovulation on progesterone concentrations in milk and blood serum and on reproductive performance. Sixty Holstein-Friesian cows averaging 8000 L over 305 days of lactation were divided into 2 groups of 30 animals. One-factor ANOVA and a t-test of progesterone concentrations in venous serum and milk revealed that the lowest concentrations of progesterone in milk (Ovsynch 5.8 + 1.0 ng/mL; control 4.5 + 0.6 ng/mL) and blood serum (Ovsynch 1.3 +/- 0.9 ng/mL; control 1.4 +/- 0.5 ng/mL) in both groups were found on the day of estrus or artificial insemination (AI) (both P < 0.05). Increases in the concentrations of progesterone in milk (Ovsynch 17.6 +/- 4.3 ng/mL; control 10.5 +/- 1.9 ng/mL) and blood serum (Ovsynch 3.6 +/- 1.1 ng/mL; control 4.0 +/- 1.0 ng/mL) were observed on day 7 after AI (both P < 0.05). Concentrations of progesterone in milk and blood serum in nonpregnant cows were reduced on day 21 after AI. Assessment of reproductive performance revealed that the application of treatment shortened the duration of the service period (Ovsynch 76 days; control 83 days) and the calving interval (Ovsynch 376 days; control 382 days).
PB  - Scientific Technical Research Council Turkey-Tubitak, Ankara
T2  - Turkish Journal of Veterinary & Animal Sciences
T1  - Progesterone concentration in milk and blood serum and reproductive efficiency of cows after Ovsynch treatment
VL  - 40
IS  - 1
SP  - 75
EP  - 80
DO  - 10.3906/vet-1505-17
ER  - 
@article{
author = "Vuković, Dejan and Božić, Aleksandar and Relić, Renata and Stančić, Blagoje and Gvozdić, Dragan and Kucević, Denis",
year = "2016",
abstract = "An experiment was conducted to investigate the effects of hormonal synchronization of ovulation on progesterone concentrations in milk and blood serum and on reproductive performance. Sixty Holstein-Friesian cows averaging 8000 L over 305 days of lactation were divided into 2 groups of 30 animals. One-factor ANOVA and a t-test of progesterone concentrations in venous serum and milk revealed that the lowest concentrations of progesterone in milk (Ovsynch 5.8 + 1.0 ng/mL; control 4.5 + 0.6 ng/mL) and blood serum (Ovsynch 1.3 +/- 0.9 ng/mL; control 1.4 +/- 0.5 ng/mL) in both groups were found on the day of estrus or artificial insemination (AI) (both P < 0.05). Increases in the concentrations of progesterone in milk (Ovsynch 17.6 +/- 4.3 ng/mL; control 10.5 +/- 1.9 ng/mL) and blood serum (Ovsynch 3.6 +/- 1.1 ng/mL; control 4.0 +/- 1.0 ng/mL) were observed on day 7 after AI (both P < 0.05). Concentrations of progesterone in milk and blood serum in nonpregnant cows were reduced on day 21 after AI. Assessment of reproductive performance revealed that the application of treatment shortened the duration of the service period (Ovsynch 76 days; control 83 days) and the calving interval (Ovsynch 376 days; control 382 days).",
publisher = "Scientific Technical Research Council Turkey-Tubitak, Ankara",
journal = "Turkish Journal of Veterinary & Animal Sciences",
title = "Progesterone concentration in milk and blood serum and reproductive efficiency of cows after Ovsynch treatment",
volume = "40",
number = "1",
pages = "75-80",
doi = "10.3906/vet-1505-17"
}
Vuković, D., Božić, A., Relić, R., Stančić, B., Gvozdić, D.,& Kucević, D.. (2016). Progesterone concentration in milk and blood serum and reproductive efficiency of cows after Ovsynch treatment. in Turkish Journal of Veterinary & Animal Sciences
Scientific Technical Research Council Turkey-Tubitak, Ankara., 40(1), 75-80.
https://doi.org/10.3906/vet-1505-17
Vuković D, Božić A, Relić R, Stančić B, Gvozdić D, Kucević D. Progesterone concentration in milk and blood serum and reproductive efficiency of cows after Ovsynch treatment. in Turkish Journal of Veterinary & Animal Sciences. 2016;40(1):75-80.
doi:10.3906/vet-1505-17 .
Vuković, Dejan, Božić, Aleksandar, Relić, Renata, Stančić, Blagoje, Gvozdić, Dragan, Kucević, Denis, "Progesterone concentration in milk and blood serum and reproductive efficiency of cows after Ovsynch treatment" in Turkish Journal of Veterinary & Animal Sciences, 40, no. 1 (2016):75-80,
https://doi.org/10.3906/vet-1505-17 . .
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Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes IgG

Fratrić, Natalija; Gvozdić, Dragan; Vuković, Dejan; Savić, Olivera; Buac, Marijana; Ilić, Vesna

(Elsevier, Amsterdam, 2012)

TY  - JOUR
AU  - Fratrić, Natalija
AU  - Gvozdić, Dragan
AU  - Vuković, Dejan
AU  - Savić, Olivera
AU  - Buac, Marijana
AU  - Ilić, Vesna
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/933
AB  - Immune complexes (IC) could have an important role in the pathogenesis of pre-ruminant calves bronchopneumonia. IC are potent activators of complement and neutrophils and they might be responsible for immune protection, as well as for pulmonary damage. Immunoglobulin G (IgG), as constituents of IC, initiates the effector phase of immune response through binding of Fc gamma and complement receptors. The oligosaccharide moieties expressed on IgG can modulate their antigen affinity and effector function. Structural characteristics of IgG molecules from IC in the pre-ruminant calves have not been studied in detail. The aim of our study was to determine if the glycosylation profile of IgG from circulating IC (CIC) in calves with bronchopneumonia differed from those of healthy control calves. A total number of 13 Holstein-Friesian calves, at the age of three months were included in the study. All calves were clinically examined by a veterinarian. Calves were classified by signs of respiratory disease in two groups: healthy (n = 6) and diseased (n = 7) calves. The CIC from calves sera were isolated by the polyethylene glycol precipitation (PEG) method. IgG molecules were isolated from PEG precipitates by Protein G affinity method. The level of expression and localization N-acetylglucosamine, galactose, sialic acid, and fucose within the isolated IgG was determined by lectin blot assay. Calves with bronchopneumonia had a statistically significantly increased level of CIC. IgG molecule:, were isolated from CIC of both healthy and diseased calves. Several other proteins in complex with IgG were detected in both groups of animals. The isolated IgG heavy chains of healthy calves expressed N-acetylglucosamine, galactose, sialic acid, and fucose. The light chains of IgG expressed N-acetylglucosamine, sialic acid, and fucose whereas galactose was not detected in healthy calves. In diseased animals, galactose was detected on light chains, and both heavy and light IgG chains were more sialylated. Proteins in complex with IgG were also lectin reactive, and their glycosylation in diseased animals was different compared to healthy controls. Increased sialylation is a characteristic of anti-inflammatory IgG. The increased sialylation of IgG from CIC in bronchopneumonia might be an attempt of immune system of calves to protect lung tissues against damages provoked by activated cells and secreted pro-inflammatory cytokines. At the same time, increased IgG sialylation could explain the inability of calves immune system to initiate the process of antigen elimination by activation of Fc gamma receptors.
PB  - Elsevier, Amsterdam
T2  - Veterinary Immunology and Immunopathology
T1  - Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes  IgG
VL  - 150
IS  - 3-4
SP  - 161
EP  - 168
DO  - 10.1016/j.vetimm.2012.09.009
ER  - 
@article{
author = "Fratrić, Natalija and Gvozdić, Dragan and Vuković, Dejan and Savić, Olivera and Buac, Marijana and Ilić, Vesna",
year = "2012",
abstract = "Immune complexes (IC) could have an important role in the pathogenesis of pre-ruminant calves bronchopneumonia. IC are potent activators of complement and neutrophils and they might be responsible for immune protection, as well as for pulmonary damage. Immunoglobulin G (IgG), as constituents of IC, initiates the effector phase of immune response through binding of Fc gamma and complement receptors. The oligosaccharide moieties expressed on IgG can modulate their antigen affinity and effector function. Structural characteristics of IgG molecules from IC in the pre-ruminant calves have not been studied in detail. The aim of our study was to determine if the glycosylation profile of IgG from circulating IC (CIC) in calves with bronchopneumonia differed from those of healthy control calves. A total number of 13 Holstein-Friesian calves, at the age of three months were included in the study. All calves were clinically examined by a veterinarian. Calves were classified by signs of respiratory disease in two groups: healthy (n = 6) and diseased (n = 7) calves. The CIC from calves sera were isolated by the polyethylene glycol precipitation (PEG) method. IgG molecules were isolated from PEG precipitates by Protein G affinity method. The level of expression and localization N-acetylglucosamine, galactose, sialic acid, and fucose within the isolated IgG was determined by lectin blot assay. Calves with bronchopneumonia had a statistically significantly increased level of CIC. IgG molecule:, were isolated from CIC of both healthy and diseased calves. Several other proteins in complex with IgG were detected in both groups of animals. The isolated IgG heavy chains of healthy calves expressed N-acetylglucosamine, galactose, sialic acid, and fucose. The light chains of IgG expressed N-acetylglucosamine, sialic acid, and fucose whereas galactose was not detected in healthy calves. In diseased animals, galactose was detected on light chains, and both heavy and light IgG chains were more sialylated. Proteins in complex with IgG were also lectin reactive, and their glycosylation in diseased animals was different compared to healthy controls. Increased sialylation is a characteristic of anti-inflammatory IgG. The increased sialylation of IgG from CIC in bronchopneumonia might be an attempt of immune system of calves to protect lung tissues against damages provoked by activated cells and secreted pro-inflammatory cytokines. At the same time, increased IgG sialylation could explain the inability of calves immune system to initiate the process of antigen elimination by activation of Fc gamma receptors.",
publisher = "Elsevier, Amsterdam",
journal = "Veterinary Immunology and Immunopathology",
title = "Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes  IgG",
volume = "150",
number = "3-4",
pages = "161-168",
doi = "10.1016/j.vetimm.2012.09.009"
}
Fratrić, N., Gvozdić, D., Vuković, D., Savić, O., Buac, M.,& Ilić, V.. (2012). Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes  IgG. in Veterinary Immunology and Immunopathology
Elsevier, Amsterdam., 150(3-4), 161-168.
https://doi.org/10.1016/j.vetimm.2012.09.009
Fratrić N, Gvozdić D, Vuković D, Savić O, Buac M, Ilić V. Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes  IgG. in Veterinary Immunology and Immunopathology. 2012;150(3-4):161-168.
doi:10.1016/j.vetimm.2012.09.009 .
Fratrić, Natalija, Gvozdić, Dragan, Vuković, Dejan, Savić, Olivera, Buac, Marijana, Ilić, Vesna, "Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes  IgG" in Veterinary Immunology and Immunopathology, 150, no. 3-4 (2012):161-168,
https://doi.org/10.1016/j.vetimm.2012.09.009 . .
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