TY  - JOUR
AU  - Prošić, Isidora
AU  - Milčić Matić, Natalija
AU  - Milić, Nenad
AU  - Radalj, Andrea
AU  - Aksentijević, Ksenija
AU  - Ilić, Milica
AU  - Nišavić, Jakov
AU  - Radojičić, Marina
AU  - Gajdov, Vladimir
AU  - Krnjaić, Dejan
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3763
AB  - The escalating global concern of antimicrobial resistance in human and veterinary
medicine is exacerbated by the inappropriate prescription of antibiotics for bacterial
infections in companion animals. This study aimed to determine the distribution of
coagulase-positive staphylococci causing clinical skin and ear infections in dogs and to
determine methicillin-resistant isolates. A total of 78 staphylococcal strains were isolated
from clinical samples taken from patients at the Dermatology Clinic at the Faculty of
Veterinary Medicine in Belgrade, Serbia. Multiplex PCR was used for species-specific
identification, and mecA and mecC genes were used to determine methicillin resistance,
in addition to phenotypic determination, MIC values and detection of PBP2a. Out of
the 78 samples analyzed, 65.8% were identified as Staphylococcus pseudintermedius, 22.4% as
S. aureus, 7.9% as S. coagulans, and 3.9% as S. intermedius. Four S. aureus isolates exhibited
methicillin resistance confirmed by cefoxitin disk diffusion, while five were confirmed
with MIC testing and latex agglutination. MecA gene was detected in 29.4% of S. aureus
and 30% of S. pseudintermedius isolates. These isolates were classified as methicillin-
resistant S. aureus (MRSA) and methicillin-resistant S. pseudintermedius (MRSP),
respectively. No isolates carried the mecC gene. This study provides insights into the
prevalence of CoPS species and methicillin resistance in isolates from dogs. Continued
surveillance is essential to monitor and understand the emergence and dissemination of
antimicrobial resistance in veterinary medicine and the results of this study accent the
need for establishment of a continuous antimicrobial resistance surveillance program
in the Republic of Serbia.
PB  - Beograd : Fakultet veterinarske medicine
T2  - Acta Veterinaria
T1  - Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study
VL  - 74
IS  - 1
SP  - 117
EP  - 132
DO  - 10.2478/acve-2024-0009
DO  - 10.2478/acve-2024-0009
ER  - 

@article{
author = "Prošić, Isidora and Milčić Matić, Natalija and Milić, Nenad and Radalj, Andrea and Aksentijević, Ksenija and Ilić, Milica and Nišavić, Jakov and Radojičić, Marina and Gajdov, Vladimir and Krnjaić, Dejan",
year = "2024",
abstract = "The escalating global concern of antimicrobial resistance in human and veterinary
medicine is exacerbated by the inappropriate prescription of antibiotics for bacterial
infections in companion animals. This study aimed to determine the distribution of
coagulase-positive staphylococci causing clinical skin and ear infections in dogs and to
determine methicillin-resistant isolates. A total of 78 staphylococcal strains were isolated
from clinical samples taken from patients at the Dermatology Clinic at the Faculty of
Veterinary Medicine in Belgrade, Serbia. Multiplex PCR was used for species-specific
identification, and mecA and mecC genes were used to determine methicillin resistance,
in addition to phenotypic determination, MIC values and detection of PBP2a. Out of
the 78 samples analyzed, 65.8% were identified as Staphylococcus pseudintermedius, 22.4% as
S. aureus, 7.9% as S. coagulans, and 3.9% as S. intermedius. Four S. aureus isolates exhibited
methicillin resistance confirmed by cefoxitin disk diffusion, while five were confirmed
with MIC testing and latex agglutination. MecA gene was detected in 29.4% of S. aureus
and 30% of S. pseudintermedius isolates. These isolates were classified as methicillin-
resistant S. aureus (MRSA) and methicillin-resistant S. pseudintermedius (MRSP),
respectively. No isolates carried the mecC gene. This study provides insights into the
prevalence of CoPS species and methicillin resistance in isolates from dogs. Continued
surveillance is essential to monitor and understand the emergence and dissemination of
antimicrobial resistance in veterinary medicine and the results of this study accent the
need for establishment of a continuous antimicrobial resistance surveillance program
in the Republic of Serbia.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Acta Veterinaria",
title = "Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study",
volume = "74",
number = "1",
pages = "117-132",
doi = "10.2478/acve-2024-0009, 10.2478/acve-2024-0009"
}

Prošić, I., Milčić Matić, N., Milić, N., Radalj, A., Aksentijević, K., Ilić, M., Nišavić, J., Radojičić, M., Gajdov, V.,& Krnjaić, D.. (2024). Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study. in Acta Veterinaria
Beograd : Fakultet veterinarske medicine., 74(1), 117-132.
https://doi.org/10.2478/acve-2024-0009

Prošić I, Milčić Matić N, Milić N, Radalj A, Aksentijević K, Ilić M, Nišavić J, Radojičić M, Gajdov V, Krnjaić D. Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study. in Acta Veterinaria. 2024;74(1):117-132.
doi:10.2478/acve-2024-0009 .

Prošić, Isidora, Milčić Matić, Natalija, Milić, Nenad, Radalj, Andrea, Aksentijević, Ksenija, Ilić, Milica, Nišavić, Jakov, Radojičić, Marina, Gajdov, Vladimir, Krnjaić, Dejan, "Molecular prevalence of MecA and MecC genеs in coagulasе-positive staphylococci isolated from dogs with dermatitis and otitis in Belgrade, Serbia: a one year study" in Acta Veterinaria, 74, no. 1 (2024):117-132,
https://doi.org/10.2478/acve-2024-0009 . .

TY  - JOUR
AU  - Ilić, Milica
AU  - Ivković, Maša
AU  - Radaković, Milena
AU  - Spariosu, Kristina
AU  - Andrić, Nenad
AU  - Kovačević Filipović, Milica
AU  - Beletić, Anđelo
AU  - Francuski Andrić, Jelena
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3254
AB  - Red blood cell osmotic fragility (RBCOF) refers to susceptibility to hemolysis in
vivo and in vitro. An increase in mean cellular hemoglobin concentration (MCHC)
also reflects in vivo and in vitro hemolysis. Common inflammatory infectious
diseases in dogs are often characterized by an increase in MCHC; however, it is
not known if there is a concomitant increase in RBCOF. The objective of this
study was to determine RBCOF in dogs with babesiosis, dirofilariosis,
parvovirosis, pyometra, and canine distemper and to investigate whether RBCOF
correlates with routine hematologic and biochemical parameters and lipoprotein
fractions. The diagnosis of canine infectious disease was made and complete
blood count, serum biochemical profiles, and lipoprotein electrophoresis were
determined according to current institutional protocols. RBCOF of healthy
control (n=8) and infected dogs (n=73) was measured with a phosphate-buffered
saline dilution test, and mean corpuscular fragility (MCF) was calculated. MCF
was increased in dirofilariosis, pyometra, and distemper; MCHC was increased
in all infected groups; HDL was decreased in babesiosis, pyometra, and
distemper compared with healthy controls. However, no correlation was found
between these parameters. On the other hand, hematological and biochemical
parameters, that are highly predictive of specific infections, correlated with MCF:
ALP for babesiosis (r=0.415, P=0.035), urea (r=-0.488, P=0.034) for
dirofilariosis, and leukocyte count (r=-0.820, P=0.046) for pyometra. Although
elevated MCHC levels in all samples from infected dogs indicate hemolysis,
RBCOF was elevated only in dirofilariosis, pyometra, and canine distemper,
leading to the conclusion that these apparently related phenomena are controlled
by different mechanisms.
PB  - Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan
T2  - Pakistan Veterinary Journal
T1  - Association of increased osmotic fragility of red blood cells with common systemic inflammatory diseases in dogs
VL  - 43
IS  - 3
SP  - 463
EP  - 469
DO  - 10.29261/pakvetj/2023.048
ER  - 

@article{
author = "Ilić, Milica and Ivković, Maša and Radaković, Milena and Spariosu, Kristina and Andrić, Nenad and Kovačević Filipović, Milica and Beletić, Anđelo and Francuski Andrić, Jelena",
year = "2023",
abstract = "Red blood cell osmotic fragility (RBCOF) refers to susceptibility to hemolysis in
vivo and in vitro. An increase in mean cellular hemoglobin concentration (MCHC)
also reflects in vivo and in vitro hemolysis. Common inflammatory infectious
diseases in dogs are often characterized by an increase in MCHC; however, it is
not known if there is a concomitant increase in RBCOF. The objective of this
study was to determine RBCOF in dogs with babesiosis, dirofilariosis,
parvovirosis, pyometra, and canine distemper and to investigate whether RBCOF
correlates with routine hematologic and biochemical parameters and lipoprotein
fractions. The diagnosis of canine infectious disease was made and complete
blood count, serum biochemical profiles, and lipoprotein electrophoresis were
determined according to current institutional protocols. RBCOF of healthy
control (n=8) and infected dogs (n=73) was measured with a phosphate-buffered
saline dilution test, and mean corpuscular fragility (MCF) was calculated. MCF
was increased in dirofilariosis, pyometra, and distemper; MCHC was increased
in all infected groups; HDL was decreased in babesiosis, pyometra, and
distemper compared with healthy controls. However, no correlation was found
between these parameters. On the other hand, hematological and biochemical
parameters, that are highly predictive of specific infections, correlated with MCF:
ALP for babesiosis (r=0.415, P=0.035), urea (r=-0.488, P=0.034) for
dirofilariosis, and leukocyte count (r=-0.820, P=0.046) for pyometra. Although
elevated MCHC levels in all samples from infected dogs indicate hemolysis,
RBCOF was elevated only in dirofilariosis, pyometra, and canine distemper,
leading to the conclusion that these apparently related phenomena are controlled
by different mechanisms.",
publisher = "Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan",
journal = "Pakistan Veterinary Journal",
title = "Association of increased osmotic fragility of red blood cells with common systemic inflammatory diseases in dogs",
volume = "43",
number = "3",
pages = "463-469",
doi = "10.29261/pakvetj/2023.048"
}

Ilić, M., Ivković, M., Radaković, M., Spariosu, K., Andrić, N., Kovačević Filipović, M., Beletić, A.,& Francuski Andrić, J.. (2023). Association of increased osmotic fragility of red blood cells with common systemic inflammatory diseases in dogs. in Pakistan Veterinary Journal
Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan., 43(3), 463-469.
https://doi.org/10.29261/pakvetj/2023.048

Ilić M, Ivković M, Radaković M, Spariosu K, Andrić N, Kovačević Filipović M, Beletić A, Francuski Andrić J. Association of increased osmotic fragility of red blood cells with common systemic inflammatory diseases in dogs. in Pakistan Veterinary Journal. 2023;43(3):463-469.
doi:10.29261/pakvetj/2023.048 .

Ilić, Milica, Ivković, Maša, Radaković, Milena, Spariosu, Kristina, Andrić, Nenad, Kovačević Filipović, Milica, Beletić, Anđelo, Francuski Andrić, Jelena, "Association of increased osmotic fragility of red blood cells with common systemic inflammatory diseases in dogs" in Pakistan Veterinary Journal, 43, no. 3 (2023):463-469,
https://doi.org/10.29261/pakvetj/2023.048 . .