TY  - JOUR
AU  - Milanović, Zorana
AU  - Ilić Božović, Anja
AU  - Bojić-Trbojević, Žanka
AU  - Hajduković, Ljiljana
AU  - Francuski Andrić, Jelena
AU  - Kovačević Filipović, Milica
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3631
AB  - Acute B. canis infection can lead to an acute phase reaction (APR) in dogs. The parasite invades red blood cells causing
anemia through immune-mediated hemolysis and possible erythropoietic suppression. A regenerative response of the
erythroid lineage during the babesiosis has not been described in extension. This research examines hematologic parameters
focusing on the absolute reticulocyte count and apolipoprotein A I (ApoA I) level on the day of admission and 14 days after
treatment with imidocarb-dipropionate in young (n=11) and adult (n=11) dogs naturally infected with B. canis. Metabolic
and inflammatory processes were characterized by analyzing protein and lipid profiles, as well as ApoA I at specified time
points. Automated analyzers were used to determine complete blood count and biochemical parameters, while ApoA I was
assessed using radioimmunoassay. The reticulocyte count was determined using a manual method by means of supravital
staining. Both young and adult dogs with acute B. canis infection showed non-regenerative anemia without difference.
Fourteen days after successful treatment with imidocarb-dipropionate, the anemia was corrected and a high reticulocyte
count was observed (p<0.05). This indicates that the erythroid regenerative response was efficient in young and adult dogs,
although vital signs, leukocyte count and triglyceride concentration suggest a more intense APR in young dogs. A decrease
in ApoA I in both groups 14 days after treatment (p<0.01) confirmed that this lipoprotein acts as a positive acute-phase
protein in acute B. canis infection in dogs, but further studies are needed to connect its role in erythroid lineage regeneration.
PB  - Saints Cyril and Methodius University of Skopje, Faculty of Veterinary Medicine
T2  - Macedonian Veterinary Review
T1  - Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs
VL  - 47
IS  - 1
DO  - 10.2478/macvetrev-2024-0011
ER  - 

@article{
author = "Milanović, Zorana and Ilić Božović, Anja and Bojić-Trbojević, Žanka and Hajduković, Ljiljana and Francuski Andrić, Jelena and Kovačević Filipović, Milica",
year = "2024",
abstract = "Acute B. canis infection can lead to an acute phase reaction (APR) in dogs. The parasite invades red blood cells causing
anemia through immune-mediated hemolysis and possible erythropoietic suppression. A regenerative response of the
erythroid lineage during the babesiosis has not been described in extension. This research examines hematologic parameters
focusing on the absolute reticulocyte count and apolipoprotein A I (ApoA I) level on the day of admission and 14 days after
treatment with imidocarb-dipropionate in young (n=11) and adult (n=11) dogs naturally infected with B. canis. Metabolic
and inflammatory processes were characterized by analyzing protein and lipid profiles, as well as ApoA I at specified time
points. Automated analyzers were used to determine complete blood count and biochemical parameters, while ApoA I was
assessed using radioimmunoassay. The reticulocyte count was determined using a manual method by means of supravital
staining. Both young and adult dogs with acute B. canis infection showed non-regenerative anemia without difference.
Fourteen days after successful treatment with imidocarb-dipropionate, the anemia was corrected and a high reticulocyte
count was observed (p<0.05). This indicates that the erythroid regenerative response was efficient in young and adult dogs,
although vital signs, leukocyte count and triglyceride concentration suggest a more intense APR in young dogs. A decrease
in ApoA I in both groups 14 days after treatment (p<0.01) confirmed that this lipoprotein acts as a positive acute-phase
protein in acute B. canis infection in dogs, but further studies are needed to connect its role in erythroid lineage regeneration.",
publisher = "Saints Cyril and Methodius University of Skopje, Faculty of Veterinary Medicine",
journal = "Macedonian Veterinary Review",
title = "Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs",
volume = "47",
number = "1",
doi = "10.2478/macvetrev-2024-0011"
}

Milanović, Z., Ilić Božović, A., Bojić-Trbojević, Ž., Hajduković, L., Francuski Andrić, J.,& Kovačević Filipović, M.. (2024). Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs. in Macedonian Veterinary Review
Saints Cyril and Methodius University of Skopje, Faculty of Veterinary Medicine., 47(1).
https://doi.org/10.2478/macvetrev-2024-0011

Milanović Z, Ilić Božović A, Bojić-Trbojević Ž, Hajduković L, Francuski Andrić J, Kovačević Filipović M. Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs. in Macedonian Veterinary Review. 2024;47(1).
doi:10.2478/macvetrev-2024-0011 .

Milanović, Zorana, Ilić Božović, Anja, Bojić-Trbojević, Žanka, Hajduković, Ljiljana, Francuski Andrić, Jelena, Kovačević Filipović, Milica, "Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs" in Macedonian Veterinary Review, 47, no. 1 (2024),
https://doi.org/10.2478/macvetrev-2024-0011 . .

TY  - JOUR
AU  - Milanović, Zorana
AU  - Vekić, Jelena
AU  - Radonjić, Vladimir
AU  - Božović-Ilić, Anja
AU  - Zeljković, Aleksandra
AU  - Janac, Jelena
AU  - Spasojevic-Kalimanovska, Vesna
AU  - Buch, Jesse
AU  - Chandrashekar, Ramaswamy
AU  - Bojic-Trbojević, Zanka
AU  - Hajduković, Ljiljana
AU  - Christopher, Mary M.
AU  - Kovačević-Filipović, Milica
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1778
AB  - Background Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results Dogs with B. canis infection had a marked APR (median SAA, 168.3 mu g/mL; range, 98.1-716.2 mu g/mL) compared with controls (3.2 mu g/mL, 2.0-4.2 mu g/mL) (P < .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P = .02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P = .02), and alpha-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P = .04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P = .02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho = .43; P = .03) and ApoA-1 (rho = .63, P < .001). Conclusions and Clinical Importance Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.
PB  - Wiley, Hoboken
T2  - Journal of Veterinary Internal Medicine
T1  - Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs
VL  - 33
IS  - 4
SP  - 1686
EP  - 1694
DO  - 10.1111/jvim.15537
ER  - 

@article{
author = "Milanović, Zorana and Vekić, Jelena and Radonjić, Vladimir and Božović-Ilić, Anja and Zeljković, Aleksandra and Janac, Jelena and Spasojevic-Kalimanovska, Vesna and Buch, Jesse and Chandrashekar, Ramaswamy and Bojic-Trbojević, Zanka and Hajduković, Ljiljana and Christopher, Mary M. and Kovačević-Filipović, Milica",
year = "2019",
abstract = "Background Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results Dogs with B. canis infection had a marked APR (median SAA, 168.3 mu g/mL; range, 98.1-716.2 mu g/mL) compared with controls (3.2 mu g/mL, 2.0-4.2 mu g/mL) (P < .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P = .02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P = .02), and alpha-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P = .04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P = .02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho = .43; P = .03) and ApoA-1 (rho = .63, P < .001). Conclusions and Clinical Importance Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.",
publisher = "Wiley, Hoboken",
journal = "Journal of Veterinary Internal Medicine",
title = "Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs",
volume = "33",
number = "4",
pages = "1686-1694",
doi = "10.1111/jvim.15537"
}

Milanović, Z., Vekić, J., Radonjić, V., Božović-Ilić, A., Zeljković, A., Janac, J., Spasojevic-Kalimanovska, V., Buch, J., Chandrashekar, R., Bojic-Trbojević, Z., Hajduković, L., Christopher, M. M.,& Kovačević-Filipović, M.. (2019). Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs. in Journal of Veterinary Internal Medicine
Wiley, Hoboken., 33(4), 1686-1694.
https://doi.org/10.1111/jvim.15537

Milanović Z, Vekić J, Radonjić V, Božović-Ilić A, Zeljković A, Janac J, Spasojevic-Kalimanovska V, Buch J, Chandrashekar R, Bojic-Trbojević Z, Hajduković L, Christopher MM, Kovačević-Filipović M. Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs. in Journal of Veterinary Internal Medicine. 2019;33(4):1686-1694.
doi:10.1111/jvim.15537 .

Milanović, Zorana, Vekić, Jelena, Radonjić, Vladimir, Božović-Ilić, Anja, Zeljković, Aleksandra, Janac, Jelena, Spasojevic-Kalimanovska, Vesna, Buch, Jesse, Chandrashekar, Ramaswamy, Bojic-Trbojević, Zanka, Hajduković, Ljiljana, Christopher, Mary M., Kovačević-Filipović, Milica, "Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs" in Journal of Veterinary Internal Medicine, 33, no. 4 (2019):1686-1694,
https://doi.org/10.1111/jvim.15537 . .