TY  - CONF
AU  - Nedić, Drago
AU  - Grković, Nevena
AU  - Kalaba, Vesna
AU  - Golić, Bojan
AU  - Ilić, Tanja
AU  - Kasagić, Dragan
AU  - Suvajdžić, Branko
AU  - Vejnović, Branislav
AU  - Đurić, Spomenka
AU  - Vasilev, Dragan
AU  - Dimitrijević, Mirjana
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2288
AB  - The aim of this study was to investigate antibacterial effects of oregano and thyme essential oils on Listeria monocytogenes in fermented sausages and their effect on the sensory characteristics of these sausages. For testing purposes, sausages contaminated with L. monocytogenes were produced. Changes in the microbiological status of fermented sausages and physicochemical properties were monitored during ripening. Essential oils exhibited antibacterial activity against L. monocytogenes, and in the groups with a high concentration (0.6%) of oregano or thyme essential oils (KLO2 and KLT2), the number of L. monocytogenes was below the detection threshold on day 14 of ripening, with a stronger effect of oregano. In groups with 0.3% essential oil of oregano or thyme added, the number of L. monocytogenes was reduced to below the detection threshold on day 21 of ripening. During the ripening, the aw and pH of all test groups of fermented sausages decreased. Experimental sausages with 0.3% thyme essential oil had acceptable smell and taste, while in other experimental groups, sausage smell and taste were very intense, uncharacteristic and unacceptable.
PB  - IOP Publishing Ltd
C3  - 61st International Meat Industry Conference (MEATCON2021)
T1  - Antimicrobial activity of thyme (Thymus vulgaris) and oregano (Origanum vulgare) essential oils against Listeria monocytogenes in fermented sausages
VL  - 854
SP  - 012064
DO  - 10.1088/1755-1315/854/1/012064
ER  - 

@conference{
author = "Nedić, Drago and Grković, Nevena and Kalaba, Vesna and Golić, Bojan and Ilić, Tanja and Kasagić, Dragan and Suvajdžić, Branko and Vejnović, Branislav and Đurić, Spomenka and Vasilev, Dragan and Dimitrijević, Mirjana",
year = "2021",
abstract = "The aim of this study was to investigate antibacterial effects of oregano and thyme essential oils on Listeria monocytogenes in fermented sausages and their effect on the sensory characteristics of these sausages. For testing purposes, sausages contaminated with L. monocytogenes were produced. Changes in the microbiological status of fermented sausages and physicochemical properties were monitored during ripening. Essential oils exhibited antibacterial activity against L. monocytogenes, and in the groups with a high concentration (0.6%) of oregano or thyme essential oils (KLO2 and KLT2), the number of L. monocytogenes was below the detection threshold on day 14 of ripening, with a stronger effect of oregano. In groups with 0.3% essential oil of oregano or thyme added, the number of L. monocytogenes was reduced to below the detection threshold on day 21 of ripening. During the ripening, the aw and pH of all test groups of fermented sausages decreased. Experimental sausages with 0.3% thyme essential oil had acceptable smell and taste, while in other experimental groups, sausage smell and taste were very intense, uncharacteristic and unacceptable.",
publisher = "IOP Publishing Ltd",
journal = "61st International Meat Industry Conference (MEATCON2021)",
title = "Antimicrobial activity of thyme (Thymus vulgaris) and oregano (Origanum vulgare) essential oils against Listeria monocytogenes in fermented sausages",
volume = "854",
pages = "012064",
doi = "10.1088/1755-1315/854/1/012064"
}

Nedić, D., Grković, N., Kalaba, V., Golić, B., Ilić, T., Kasagić, D., Suvajdžić, B., Vejnović, B., Đurić, S., Vasilev, D.,& Dimitrijević, M.. (2021). Antimicrobial activity of thyme (Thymus vulgaris) and oregano (Origanum vulgare) essential oils against Listeria monocytogenes in fermented sausages. in 61st International Meat Industry Conference (MEATCON2021)
IOP Publishing Ltd., 854, 012064.
https://doi.org/10.1088/1755-1315/854/1/012064

Nedić D, Grković N, Kalaba V, Golić B, Ilić T, Kasagić D, Suvajdžić B, Vejnović B, Đurić S, Vasilev D, Dimitrijević M. Antimicrobial activity of thyme (Thymus vulgaris) and oregano (Origanum vulgare) essential oils against Listeria monocytogenes in fermented sausages. in 61st International Meat Industry Conference (MEATCON2021). 2021;854:012064.
doi:10.1088/1755-1315/854/1/012064 .

Nedić, Drago, Grković, Nevena, Kalaba, Vesna, Golić, Bojan, Ilić, Tanja, Kasagić, Dragan, Suvajdžić, Branko, Vejnović, Branislav, Đurić, Spomenka, Vasilev, Dragan, Dimitrijević, Mirjana, "Antimicrobial activity of thyme (Thymus vulgaris) and oregano (Origanum vulgare) essential oils against Listeria monocytogenes in fermented sausages" in 61st International Meat Industry Conference (MEATCON2021), 854 (2021):012064,
https://doi.org/10.1088/1755-1315/854/1/012064 . .

TY  - CONF
AU  - Kirovski, Danijela
AU  - Knežević, Dragan
AU  - Golić, Bojan
AU  - Kasagić, Dragan
AU  - Jovanović, Ljubomir
AU  - Bošnjaković, Dušan
AU  - Trifković, Julijana
AU  - Sladojević, Željko
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2274
AB  - Cow milk is widely consumed by human children and adults due to its nutritional value. Besides
nutrients, milk contains naturally occurring hormones, including insulin like growth factor-1
(IGF-1), that may alter their blood levels in the consumers. At the same time, the impact of IGF1 on human health is still unclear and controversial. The aim of the study was to determine IGF-1
concentrations in cow colostrum and milk and to discuss them from the aspect of human health.
Twenty Holstein cows were enrolled in the study and subjected to colostrum, milk and venous
blood sampling in order to determine the IGF-1 concentration by the radioimmunoassay (RIA).
Colostrum was sampled at 2, 14 and 26 hours (1st, 2nd and 3rd colostrum) after calving, while
milk and venous blood were taken 10 days after calving. The concentration of IGF-1 was the
highest in the 1
st colostrum and decreased significantly with each subsequent sampling, so the
lowest concentrations were detected in milk sampled 10 days after calving. High concentrations
of IGF-1 observed in colostrum, especially in the 1st colostrum, reflect its potential for eventually
use in the treatment of various intestinal diseases. However, further research should determine
the conditions and limitations of its application. On the other hand, additional research is needed
to determine whether IGF-1 in milk has harmful effects on human health and whether it is
necessary to limit the permitted presence of this hormone in cow milk.
PB  - Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - Proceedings of the XII International Scientific Agricultural Symposium „Agrosym 2021“
T1  - Cow milk insulin like growth factor-1: risk or benefit for human health?
SP  - 1196
EP  - 1203
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2274
ER  - 

@conference{
author = "Kirovski, Danijela and Knežević, Dragan and Golić, Bojan and Kasagić, Dragan and Jovanović, Ljubomir and Bošnjaković, Dušan and Trifković, Julijana and Sladojević, Željko",
year = "2021",
abstract = "Cow milk is widely consumed by human children and adults due to its nutritional value. Besides
nutrients, milk contains naturally occurring hormones, including insulin like growth factor-1
(IGF-1), that may alter their blood levels in the consumers. At the same time, the impact of IGF1 on human health is still unclear and controversial. The aim of the study was to determine IGF-1
concentrations in cow colostrum and milk and to discuss them from the aspect of human health.
Twenty Holstein cows were enrolled in the study and subjected to colostrum, milk and venous
blood sampling in order to determine the IGF-1 concentration by the radioimmunoassay (RIA).
Colostrum was sampled at 2, 14 and 26 hours (1st, 2nd and 3rd colostrum) after calving, while
milk and venous blood were taken 10 days after calving. The concentration of IGF-1 was the
highest in the 1
st colostrum and decreased significantly with each subsequent sampling, so the
lowest concentrations were detected in milk sampled 10 days after calving. High concentrations
of IGF-1 observed in colostrum, especially in the 1st colostrum, reflect its potential for eventually
use in the treatment of various intestinal diseases. However, further research should determine
the conditions and limitations of its application. On the other hand, additional research is needed
to determine whether IGF-1 in milk has harmful effects on human health and whether it is
necessary to limit the permitted presence of this hormone in cow milk.",
publisher = "Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "Proceedings of the XII International Scientific Agricultural Symposium „Agrosym 2021“",
title = "Cow milk insulin like growth factor-1: risk or benefit for human health?",
pages = "1196-1203",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2274"
}

Kirovski, D., Knežević, D., Golić, B., Kasagić, D., Jovanović, L., Bošnjaković, D., Trifković, J.,& Sladojević, Ž.. (2021). Cow milk insulin like growth factor-1: risk or benefit for human health?. in Proceedings of the XII International Scientific Agricultural Symposium „Agrosym 2021“
Faculty of Agriculture, Republic of Srpska, Bosnia., 1196-1203.
https://hdl.handle.net/21.15107/rcub_veterinar_2274

Kirovski D, Knežević D, Golić B, Kasagić D, Jovanović L, Bošnjaković D, Trifković J, Sladojević Ž. Cow milk insulin like growth factor-1: risk or benefit for human health?. in Proceedings of the XII International Scientific Agricultural Symposium „Agrosym 2021“. 2021;:1196-1203.
https://hdl.handle.net/21.15107/rcub_veterinar_2274 .

Kirovski, Danijela, Knežević, Dragan, Golić, Bojan, Kasagić, Dragan, Jovanović, Ljubomir, Bošnjaković, Dušan, Trifković, Julijana, Sladojević, Željko, "Cow milk insulin like growth factor-1: risk or benefit for human health?" in Proceedings of the XII International Scientific Agricultural Symposium „Agrosym 2021“ (2021):1196-1203,
https://hdl.handle.net/21.15107/rcub_veterinar_2274 .

TY  - CONF
AU  - Knežević, Dragan
AU  - Nedić, Sreten
AU  - Jovanović, Ljubomir
AU  - Bošnjaković, Dušan
AU  - Golić, Bojan
AU  - Kasagić, Dragan
AU  - Kirovski, Danijela
AU  - Sladojević, Željko
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2276
AB  - Кортизол је присутан у млеку крава и потиче из крви у којој његова
концентрација расте као одговор на акутни стрес. С обзиром да краве често не
показују јасне знаке стресног стања, значајно је утврдити дијагностичке
биомаркере који би указали да се јединка налази у стању стреса. Један од
најзначајнијих биомаркера стреса је концентрација кортизола у крви крава.
Међутим, узорковање крви изазива стрес, тако да би биомаркери добијени из
билошких течности које се узоркују без изазивања стреса били много вреднији
за дијагностику стресног стања. У том смислу узорак избора је млеко, јер се
његово узорковање врши без манипулације са животињом, односно у
потпуности је у складу са препорукама везаним за добробит животиња.
Концентрација кортизола у млеку је нижа него у крви и износи око 4 %
концентрације у крвној плазми с обзиром да само слободна форма хормона
прелази из крви у млеко и да ћелије млечне жлезде садрже високо афинитетне
рецепторе за овај хормон који га везују при његовом преласку из крви у млеко.
У млеку се налази везујући протеин за кортикостероиде који доспева из крви а
затим везује извесну количину кортизола млека. Његова заступљеност у млеку
је различита у различитим фазама лактације. Концентрација слободног
кортизола у млеку је у високој позитивној корелацији са концентрацијом слободног кортизола у крви док концентрација везаног кортизола млека не показује тако висок степен корелације са концентрацијом везаног кортизола у крви. Методологија одређивања концентрација како слобоног тако и везаног кортизола у млеку крава је успостављена и потврђена у нашим резултатима због чега је оправдано препоручити млеко као приоритетни биолошки
материјал за одређивање кортизола као биомаркера акутног стреса код крава.
Додатно, наши резултати указују да је након екстракције кортизола најбоље
применити RIA (eng. radioimmunoassay) методу с обзиром на ниске
концентрације кортизола у млеку током каснијих фаза лактације које
захтевају високу осетљивост методе да би били детектовани.
AB  - Cortisol is present in cows’ milk and originated from blood in which its
concentration increase due to acute stress. As cows do not always show clear signs
of dicomfort, reliable diagnostic biomarker could be used to provide information
regarding cows’ stress. One of the most relaible biomarker is blood cortisol
concentration. However, blood sampling usually provoke stress, and therefore
biomarkers that originated from biological fluids that might be sampling without
stress would be more valiable for stress diagnostic. Milk can be viewed as the
sampling site of first choice, since it could be measured without manipulation of
animals, hence it is completely compatible with animal welfare recomendations.
Milk cortisol concentration is lower than in blood and is 4% of blood
concentration since only free form transfer from blood to milk and mammary cells
have high-affinity receptors that binds hormone during its transfer from blood to
milk. There is corticoid binding protein in milk that originates from blood and that
bind some of cortisol in milk. Its abundance in milk is different in different stages
of lactation. Free milk cortisol concentration is highly positively correlated with
free blood cortisol concentration, while bound milk cortisol concentration is not
highly positively correlated with bound blood cortisol concentration.
Methodology of determination of both free and bound cortisol in cows’ milk is established and confirmed in our results and thus it is justified to recomend milk
and biological material of choice for deterining cortisol as acute stress biomarker
in cows. Additionally, our results indicate that it is best to use RIA
(radioimmunoassay) method, after extraction of cortisol, as low concentrations of
milk cortisol during later phases of lactation need high sensitive method in order
to be detected.
C3  - 26th Annual Counselling of Doctors of Veterinary Medicine of Republic of Srpska (Bosnia and Hercegovina)
T1  - Kortizol mleka kao indikator stresa kod krava
T1  - Milk cortisol as stress indicator in cows
SP  - 141
EP  - 144
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2276
ER  - 

@conference{
author = "Knežević, Dragan and Nedić, Sreten and Jovanović, Ljubomir and Bošnjaković, Dušan and Golić, Bojan and Kasagić, Dragan and Kirovski, Danijela and Sladojević, Željko",
year = "2021",
abstract = "Кортизол је присутан у млеку крава и потиче из крви у којој његова
концентрација расте као одговор на акутни стрес. С обзиром да краве често не
показују јасне знаке стресног стања, значајно је утврдити дијагностичке
биомаркере који би указали да се јединка налази у стању стреса. Један од
најзначајнијих биомаркера стреса је концентрација кортизола у крви крава.
Међутим, узорковање крви изазива стрес, тако да би биомаркери добијени из
билошких течности које се узоркују без изазивања стреса били много вреднији
за дијагностику стресног стања. У том смислу узорак избора је млеко, јер се
његово узорковање врши без манипулације са животињом, односно у
потпуности је у складу са препорукама везаним за добробит животиња.
Концентрација кортизола у млеку је нижа него у крви и износи око 4 %
концентрације у крвној плазми с обзиром да само слободна форма хормона
прелази из крви у млеко и да ћелије млечне жлезде садрже високо афинитетне
рецепторе за овај хормон који га везују при његовом преласку из крви у млеко.
У млеку се налази везујући протеин за кортикостероиде који доспева из крви а
затим везује извесну количину кортизола млека. Његова заступљеност у млеку
је различита у различитим фазама лактације. Концентрација слободног
кортизола у млеку је у високој позитивној корелацији са концентрацијом слободног кортизола у крви док концентрација везаног кортизола млека не показује тако висок степен корелације са концентрацијом везаног кортизола у крви. Методологија одређивања концентрација како слобоног тако и везаног кортизола у млеку крава је успостављена и потврђена у нашим резултатима због чега је оправдано препоручити млеко као приоритетни биолошки
материјал за одређивање кортизола као биомаркера акутног стреса код крава.
Додатно, наши резултати указују да је након екстракције кортизола најбоље
применити RIA (eng. radioimmunoassay) методу с обзиром на ниске
концентрације кортизола у млеку током каснијих фаза лактације које
захтевају високу осетљивост методе да би били детектовани., Cortisol is present in cows’ milk and originated from blood in which its
concentration increase due to acute stress. As cows do not always show clear signs
of dicomfort, reliable diagnostic biomarker could be used to provide information
regarding cows’ stress. One of the most relaible biomarker is blood cortisol
concentration. However, blood sampling usually provoke stress, and therefore
biomarkers that originated from biological fluids that might be sampling without
stress would be more valiable for stress diagnostic. Milk can be viewed as the
sampling site of first choice, since it could be measured without manipulation of
animals, hence it is completely compatible with animal welfare recomendations.
Milk cortisol concentration is lower than in blood and is 4% of blood
concentration since only free form transfer from blood to milk and mammary cells
have high-affinity receptors that binds hormone during its transfer from blood to
milk. There is corticoid binding protein in milk that originates from blood and that
bind some of cortisol in milk. Its abundance in milk is different in different stages
of lactation. Free milk cortisol concentration is highly positively correlated with
free blood cortisol concentration, while bound milk cortisol concentration is not
highly positively correlated with bound blood cortisol concentration.
Methodology of determination of both free and bound cortisol in cows’ milk is established and confirmed in our results and thus it is justified to recomend milk
and biological material of choice for deterining cortisol as acute stress biomarker
in cows. Additionally, our results indicate that it is best to use RIA
(radioimmunoassay) method, after extraction of cortisol, as low concentrations of
milk cortisol during later phases of lactation need high sensitive method in order
to be detected.",
journal = "26th Annual Counselling of Doctors of Veterinary Medicine of Republic of Srpska (Bosnia and Hercegovina)",
title = "Kortizol mleka kao indikator stresa kod krava, Milk cortisol as stress indicator in cows",
pages = "141-144",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2276"
}

Knežević, D., Nedić, S., Jovanović, L., Bošnjaković, D., Golić, B., Kasagić, D., Kirovski, D.,& Sladojević, Ž.. (2021). Kortizol mleka kao indikator stresa kod krava. in 26th Annual Counselling of Doctors of Veterinary Medicine of Republic of Srpska (Bosnia and Hercegovina), 141-144.
https://hdl.handle.net/21.15107/rcub_veterinar_2276

Knežević D, Nedić S, Jovanović L, Bošnjaković D, Golić B, Kasagić D, Kirovski D, Sladojević Ž. Kortizol mleka kao indikator stresa kod krava. in 26th Annual Counselling of Doctors of Veterinary Medicine of Republic of Srpska (Bosnia and Hercegovina). 2021;:141-144.
https://hdl.handle.net/21.15107/rcub_veterinar_2276 .

Knežević, Dragan, Nedić, Sreten, Jovanović, Ljubomir, Bošnjaković, Dušan, Golić, Bojan, Kasagić, Dragan, Kirovski, Danijela, Sladojević, Željko, "Kortizol mleka kao indikator stresa kod krava" in 26th Annual Counselling of Doctors of Veterinary Medicine of Republic of Srpska (Bosnia and Hercegovina) (2021):141-144,
https://hdl.handle.net/21.15107/rcub_veterinar_2276 .