TY  - JOUR
AU  - Milanović, Zorana
AU  - Ilić Božović, Anja
AU  - Bojić-Trbojević, Žanka
AU  - Hajduković, Ljiljana
AU  - Francuski Andrić, Jelena
AU  - Kovačević Filipović, Milica
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3631
AB  - Acute B. canis infection can lead to an acute phase reaction (APR) in dogs. The parasite invades red blood cells causing
anemia through immune-mediated hemolysis and possible erythropoietic suppression. A regenerative response of the
erythroid lineage during the babesiosis has not been described in extension. This research examines hematologic parameters
focusing on the absolute reticulocyte count and apolipoprotein A I (ApoA I) level on the day of admission and 14 days after
treatment with imidocarb-dipropionate in young (n=11) and adult (n=11) dogs naturally infected with B. canis. Metabolic
and inflammatory processes were characterized by analyzing protein and lipid profiles, as well as ApoA I at specified time
points. Automated analyzers were used to determine complete blood count and biochemical parameters, while ApoA I was
assessed using radioimmunoassay. The reticulocyte count was determined using a manual method by means of supravital
staining. Both young and adult dogs with acute B. canis infection showed non-regenerative anemia without difference.
Fourteen days after successful treatment with imidocarb-dipropionate, the anemia was corrected and a high reticulocyte
count was observed (p<0.05). This indicates that the erythroid regenerative response was efficient in young and adult dogs,
although vital signs, leukocyte count and triglyceride concentration suggest a more intense APR in young dogs. A decrease
in ApoA I in both groups 14 days after treatment (p<0.01) confirmed that this lipoprotein acts as a positive acute-phase
protein in acute B. canis infection in dogs, but further studies are needed to connect its role in erythroid lineage regeneration.
PB  - Saints Cyril and Methodius University of Skopje, Faculty of Veterinary Medicine
T2  - Macedonian Veterinary Review
T1  - Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs
VL  - 47
IS  - 1
DO  - 10.2478/macvetrev-2024-0011
ER  - 

@article{
author = "Milanović, Zorana and Ilić Božović, Anja and Bojić-Trbojević, Žanka and Hajduković, Ljiljana and Francuski Andrić, Jelena and Kovačević Filipović, Milica",
year = "2024",
abstract = "Acute B. canis infection can lead to an acute phase reaction (APR) in dogs. The parasite invades red blood cells causing
anemia through immune-mediated hemolysis and possible erythropoietic suppression. A regenerative response of the
erythroid lineage during the babesiosis has not been described in extension. This research examines hematologic parameters
focusing on the absolute reticulocyte count and apolipoprotein A I (ApoA I) level on the day of admission and 14 days after
treatment with imidocarb-dipropionate in young (n=11) and adult (n=11) dogs naturally infected with B. canis. Metabolic
and inflammatory processes were characterized by analyzing protein and lipid profiles, as well as ApoA I at specified time
points. Automated analyzers were used to determine complete blood count and biochemical parameters, while ApoA I was
assessed using radioimmunoassay. The reticulocyte count was determined using a manual method by means of supravital
staining. Both young and adult dogs with acute B. canis infection showed non-regenerative anemia without difference.
Fourteen days after successful treatment with imidocarb-dipropionate, the anemia was corrected and a high reticulocyte
count was observed (p<0.05). This indicates that the erythroid regenerative response was efficient in young and adult dogs,
although vital signs, leukocyte count and triglyceride concentration suggest a more intense APR in young dogs. A decrease
in ApoA I in both groups 14 days after treatment (p<0.01) confirmed that this lipoprotein acts as a positive acute-phase
protein in acute B. canis infection in dogs, but further studies are needed to connect its role in erythroid lineage regeneration.",
publisher = "Saints Cyril and Methodius University of Skopje, Faculty of Veterinary Medicine",
journal = "Macedonian Veterinary Review",
title = "Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs",
volume = "47",
number = "1",
doi = "10.2478/macvetrev-2024-0011"
}

Milanović, Z., Ilić Božović, A., Bojić-Trbojević, Ž., Hajduković, L., Francuski Andrić, J.,& Kovačević Filipović, M.. (2024). Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs. in Macedonian Veterinary Review
Saints Cyril and Methodius University of Skopje, Faculty of Veterinary Medicine., 47(1).
https://doi.org/10.2478/macvetrev-2024-0011

Milanović Z, Ilić Božović A, Bojić-Trbojević Ž, Hajduković L, Francuski Andrić J, Kovačević Filipović M. Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs. in Macedonian Veterinary Review. 2024;47(1).
doi:10.2478/macvetrev-2024-0011 .

Milanović, Zorana, Ilić Božović, Anja, Bojić-Trbojević, Žanka, Hajduković, Ljiljana, Francuski Andrić, Jelena, Kovačević Filipović, Milica, "Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs" in Macedonian Veterinary Review, 47, no. 1 (2024),
https://doi.org/10.2478/macvetrev-2024-0011 . .

TY  - JOUR
AU  - Pirković, Andrea
AU  - Vilotić, Aleksandra
AU  - Borozan, Sunčica
AU  - Nacka-Aleksić, Mirjana
AU  - Bojić-Trbojević, Žanka
AU  - Jovanović-Krivokuća, Milica
AU  - Battino, Maurizio
AU  - Giampieri, Francesca
AU  - Dekanski, Dragana
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2539
AB  - Olive-derived bioactive compound oleuropein was evaluated against damage induced by hydrogen peroxide in human trophoblast cells in vitro, by examining the changes in several markers implicated in oxidative stress interactions in the placenta. Trophoblast HTR-8/SVneo cells were preincubated with OLE at 10 and 100 µM and exposed to H2O2, as a model of oxidative stress. Protein and lipid peroxidation, as well as antioxidant enzymes’ activity, were determined spectrophotometrically, and DNA damage was evaluated by comet assay. iNOS protein expression was assessed by Western blot, while the mRNA expression of pro- and anti-apoptotic genes BAX and BCL2 and transcription factor NFE2L2, as well as cytokines IL-6 and TNF α were determined by qPCR. Oleuropein demonstrated cytoprotective effects against H2O2 in trophoblast cells by significantly improving the antioxidant status and preventing protein and lipid damage, as well as reducing the iNOS levels. OLE reduced the mRNA expression of IL-6 and TNF α, however, it did not influence the expression of NFE2L2 or the BAX/BCL2 ratio after H2O2 exposure. Oleuropein per se did not lead to any adverse effects in HTR-8/SVneo cells under the described conditions, confirming its safety in vitro. In conclusion, it significantly attenuated oxidative damage and restored antioxidant functioning, confirming its protective role in trophoblast. © 2023 by the authors.
PB  - MDPI
T2  - Antioxidants
T1  - Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells
VL  - 12
VL  - 1
SP  - 197
DO  - 10.3390/antiox12010197
ER  - 

@article{
author = "Pirković, Andrea and Vilotić, Aleksandra and Borozan, Sunčica and Nacka-Aleksić, Mirjana and Bojić-Trbojević, Žanka and Jovanović-Krivokuća, Milica and Battino, Maurizio and Giampieri, Francesca and Dekanski, Dragana",
year = "2023",
abstract = "Olive-derived bioactive compound oleuropein was evaluated against damage induced by hydrogen peroxide in human trophoblast cells in vitro, by examining the changes in several markers implicated in oxidative stress interactions in the placenta. Trophoblast HTR-8/SVneo cells were preincubated with OLE at 10 and 100 µM and exposed to H2O2, as a model of oxidative stress. Protein and lipid peroxidation, as well as antioxidant enzymes’ activity, were determined spectrophotometrically, and DNA damage was evaluated by comet assay. iNOS protein expression was assessed by Western blot, while the mRNA expression of pro- and anti-apoptotic genes BAX and BCL2 and transcription factor NFE2L2, as well as cytokines IL-6 and TNF α were determined by qPCR. Oleuropein demonstrated cytoprotective effects against H2O2 in trophoblast cells by significantly improving the antioxidant status and preventing protein and lipid damage, as well as reducing the iNOS levels. OLE reduced the mRNA expression of IL-6 and TNF α, however, it did not influence the expression of NFE2L2 or the BAX/BCL2 ratio after H2O2 exposure. Oleuropein per se did not lead to any adverse effects in HTR-8/SVneo cells under the described conditions, confirming its safety in vitro. In conclusion, it significantly attenuated oxidative damage and restored antioxidant functioning, confirming its protective role in trophoblast. © 2023 by the authors.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells",
volume = "12, 1",
pages = "197",
doi = "10.3390/antiox12010197"
}

Pirković, A., Vilotić, A., Borozan, S., Nacka-Aleksić, M., Bojić-Trbojević, Ž., Jovanović-Krivokuća, M., Battino, M., Giampieri, F.,& Dekanski, D.. (2023). Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells. in Antioxidants
MDPI., 12, 197.
https://doi.org/10.3390/antiox12010197

Pirković A, Vilotić A, Borozan S, Nacka-Aleksić M, Bojić-Trbojević Ž, Jovanović-Krivokuća M, Battino M, Giampieri F, Dekanski D. Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells. in Antioxidants. 2023;12:197.
doi:10.3390/antiox12010197 .

Pirković, Andrea, Vilotić, Aleksandra, Borozan, Sunčica, Nacka-Aleksić, Mirjana, Bojić-Trbojević, Žanka, Jovanović-Krivokuća, Milica, Battino, Maurizio, Giampieri, Francesca, Dekanski, Dragana, "Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells" in Antioxidants, 12 (2023):197,
https://doi.org/10.3390/antiox12010197 . .