TY  - CONF
AU  - Vakanjac, Slobodanka
AU  - Maletić, Milan
AU  - Magaš, Vladimir
AU  - Nedić, Svetlana
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3724
AB  - Cilj rada bio je ispitivanje i poređenje parametara kinetike i pokretljivosti
spermatozoida četiri rase nerastova. Uključeno je bilo 34
nerasta: 8 pietrena, 10 jorkšira, 8 duroka i 8 landrasa, čije je seme
analizirano kompjuterski asistiranom analizom (CASA). Poređenjem
između rasa utvrđena je značajno viša ukupna i progresivana pokretljivost
kod duroka u poređenju sa rasom jorkšir (P < 0,05). Procenat
brzih spermatozoida bio je značajno viši kod rase durok u poređenju
sa rasom landras (P < 0,05). Kod rase landras utvrđena je značajno
viša (P < 0,05) procentualna zastupljenost sporih spermatozoida u poređenju
sa rasom durok. Vrednosti VCL kod duroka (P < 0,05) bile su
značajno više u poređenju sa landrasom. Signifikantno više vrednosti
(P < 0,05) VSL, VAP i DCL ustanovljene su kod rase durok u odnosu
na jokšir. Značajno više vrednosti ALH utvrđene su kod rase durok u
poređenju sa rasama jorkšir i landras (P < 0,01) i rasom pitren (P <
0,05). Značajno više vrednosti (P < 0,05) BCF utvrđene su kod rase
landras u poređenju sa rasom jorkšir. Rasa ima značajan uticaj kako
na osnovne parametre pokretljivosti spermatozoida tako i na sve ispitivane
parametre kinetike spermatozoida.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024
T1  - Analiza parametara pokretljivosti i kinetike spermatozoida između rasa nerastova
SP  - 141
EP  - 148
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3724
ER  - 

@conference{
author = "Vakanjac, Slobodanka and Maletić, Milan and Magaš, Vladimir and Nedić, Svetlana",
year = "2024",
abstract = "Cilj rada bio je ispitivanje i poređenje parametara kinetike i pokretljivosti
spermatozoida četiri rase nerastova. Uključeno je bilo 34
nerasta: 8 pietrena, 10 jorkšira, 8 duroka i 8 landrasa, čije je seme
analizirano kompjuterski asistiranom analizom (CASA). Poređenjem
između rasa utvrđena je značajno viša ukupna i progresivana pokretljivost
kod duroka u poređenju sa rasom jorkšir (P < 0,05). Procenat
brzih spermatozoida bio je značajno viši kod rase durok u poređenju
sa rasom landras (P < 0,05). Kod rase landras utvrđena je značajno
viša (P < 0,05) procentualna zastupljenost sporih spermatozoida u poređenju
sa rasom durok. Vrednosti VCL kod duroka (P < 0,05) bile su
značajno više u poređenju sa landrasom. Signifikantno više vrednosti
(P < 0,05) VSL, VAP i DCL ustanovljene su kod rase durok u odnosu
na jokšir. Značajno više vrednosti ALH utvrđene su kod rase durok u
poređenju sa rasama jorkšir i landras (P < 0,01) i rasom pitren (P <
0,05). Značajno više vrednosti (P < 0,05) BCF utvrđene su kod rase
landras u poređenju sa rasom jorkšir. Rasa ima značajan uticaj kako
na osnovne parametre pokretljivosti spermatozoida tako i na sve ispitivane
parametre kinetike spermatozoida.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024",
title = "Analiza parametara pokretljivosti i kinetike spermatozoida između rasa nerastova",
pages = "141-148",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3724"
}

Vakanjac, S., Maletić, M., Magaš, V.,& Nedić, S.. (2024). Analiza parametara pokretljivosti i kinetike spermatozoida između rasa nerastova. in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 141-148.
https://hdl.handle.net/21.15107/rcub_veterinar_3724

Vakanjac S, Maletić M, Magaš V, Nedić S. Analiza parametara pokretljivosti i kinetike spermatozoida između rasa nerastova. in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024. 2024;:141-148.
https://hdl.handle.net/21.15107/rcub_veterinar_3724 .

Vakanjac, Slobodanka, Maletić, Milan, Magaš, Vladimir, Nedić, Svetlana, "Analiza parametara pokretljivosti i kinetike spermatozoida između rasa nerastova" in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024 (2024):141-148,
https://hdl.handle.net/21.15107/rcub_veterinar_3724 .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Nedić, Sreten
AU  - Bošnjaković, Dušan
AU  - Nedić, Svetlana
AU  - Jovanović, Ljubomir
AU  - Arsić, Sveta
AU  - Bojkovski, Jovan
AU  - Borozan, Sunčica
AU  - Kirovski, Danijela
AU  - Vujanac, Ivan
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3784
AB  - Due to their antiketogenic and antioxidant effects, chestnut
tannins may offer a viable approach to manage the impaired immune and
renal functions in transition cows. This study aimed to investigate the effects of
dietary supplementation with chestnut tannins on haematological, biochemical
and antioxidant indices, as well as cortisol levels in prepartum dairy cows.
Forty multiparous Holstein cows were divided into two homogeneous groups
(n = 20): a control (CON), and an experimental group (CNT) receiving
20 g/day of chestnut tannins for the last 25 ± 2 days of pregnancy. Haematological
and biochemical indices, cortisol concentration and total antioxidant capacity
(T-AOC) were measured in blood samples collected 25 (day −25) and 5 days
(day −5) before the expected parturition. The addition of chestnut tannins
exerted no significant effect on red blood cells indices; however, white blood cell
(P = 0.02), lymphocyte (P = 0.05) and platelet (P < 0.01) counts were higher,
while the neutrophil to lymphocyte ratio (P = 0.03) was lower on day −5 in the
CNT group compared to the CON group. Significantly higher values of T-AOC
(P = 0.03) and significantly lower levels of triglycerides (P = 0.03) and gammaglutamyl
transferase (P = 0.02) were also found in CNT compared to CON on
day −5. The improved haematological profile in CNT cows was accompanied
by lower serum creatinine concentration (P = 0.04), while total protein, calcium,
phosphorus and cortisol did not differ significantly between CNT and CON cows.
These data demonstrate that dietary chestnut tannin supplementation in a closeup
diet has antioxidant and anti-inflammatory effects, and could potentially mitigate
immune suppression and kidney dysfunction near parturition. Further research
should be conducted concerning the mechanisms underlying these responses.
PB  - Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences in Jabłonna (Poland)
PB  - Kielanowski
T2  - Journal of Animal and Feed Sciences
T1  - Chestnut tannin supplementation can improve immune response and kidney function in prepartum dairy cows
VL  - 33
IS  - 2
SP  - 185
EP  - 192
DO  - 10.22358/jafs/174065/2023
ER  - 

@article{
author = "Prodanović, Radiša and Nedić, Sreten and Bošnjaković, Dušan and Nedić, Svetlana and Jovanović, Ljubomir and Arsić, Sveta and Bojkovski, Jovan and Borozan, Sunčica and Kirovski, Danijela and Vujanac, Ivan",
year = "2024",
abstract = "Due to their antiketogenic and antioxidant effects, chestnut
tannins may offer a viable approach to manage the impaired immune and
renal functions in transition cows. This study aimed to investigate the effects of
dietary supplementation with chestnut tannins on haematological, biochemical
and antioxidant indices, as well as cortisol levels in prepartum dairy cows.
Forty multiparous Holstein cows were divided into two homogeneous groups
(n = 20): a control (CON), and an experimental group (CNT) receiving
20 g/day of chestnut tannins for the last 25 ± 2 days of pregnancy. Haematological
and biochemical indices, cortisol concentration and total antioxidant capacity
(T-AOC) were measured in blood samples collected 25 (day −25) and 5 days
(day −5) before the expected parturition. The addition of chestnut tannins
exerted no significant effect on red blood cells indices; however, white blood cell
(P = 0.02), lymphocyte (P = 0.05) and platelet (P < 0.01) counts were higher,
while the neutrophil to lymphocyte ratio (P = 0.03) was lower on day −5 in the
CNT group compared to the CON group. Significantly higher values of T-AOC
(P = 0.03) and significantly lower levels of triglycerides (P = 0.03) and gammaglutamyl
transferase (P = 0.02) were also found in CNT compared to CON on
day −5. The improved haematological profile in CNT cows was accompanied
by lower serum creatinine concentration (P = 0.04), while total protein, calcium,
phosphorus and cortisol did not differ significantly between CNT and CON cows.
These data demonstrate that dietary chestnut tannin supplementation in a closeup
diet has antioxidant and anti-inflammatory effects, and could potentially mitigate
immune suppression and kidney dysfunction near parturition. Further research
should be conducted concerning the mechanisms underlying these responses.",
publisher = "Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences in Jabłonna (Poland), Kielanowski",
journal = "Journal of Animal and Feed Sciences",
title = "Chestnut tannin supplementation can improve immune response and kidney function in prepartum dairy cows",
volume = "33",
number = "2",
pages = "185-192",
doi = "10.22358/jafs/174065/2023"
}

Prodanović, R., Nedić, S., Bošnjaković, D., Nedić, S., Jovanović, L., Arsić, S., Bojkovski, J., Borozan, S., Kirovski, D.,& Vujanac, I.. (2024). Chestnut tannin supplementation can improve immune response and kidney function in prepartum dairy cows. in Journal of Animal and Feed Sciences
Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences in Jabłonna (Poland)., 33(2), 185-192.
https://doi.org/10.22358/jafs/174065/2023

Prodanović R, Nedić S, Bošnjaković D, Nedić S, Jovanović L, Arsić S, Bojkovski J, Borozan S, Kirovski D, Vujanac I. Chestnut tannin supplementation can improve immune response and kidney function in prepartum dairy cows. in Journal of Animal and Feed Sciences. 2024;33(2):185-192.
doi:10.22358/jafs/174065/2023 .

Prodanović, Radiša, Nedić, Sreten, Bošnjaković, Dušan, Nedić, Svetlana, Jovanović, Ljubomir, Arsić, Sveta, Bojkovski, Jovan, Borozan, Sunčica, Kirovski, Danijela, Vujanac, Ivan, "Chestnut tannin supplementation can improve immune response and kidney function in prepartum dairy cows" in Journal of Animal and Feed Sciences, 33, no. 2 (2024):185-192,
https://doi.org/10.22358/jafs/174065/2023 . .

TY  - CONF
AU  - Vakanjac, Slobodanka
AU  - Nedić, Svetlana
AU  - Magaš, Vladimir
AU  - Blagojević, Jovan
AU  - Maletić, Milan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3019
AB  - Veliki ekonomski razvoj, rast i mobilnost stanovništva, povećali su potražnju za
mlečnim i mesnim proizvodima, ali su takođe izvršili pritisak na održivost ruralnih sredina i menadžment proizvodnje hrane. Uzgajivači stoke bi trebalo da
povećaju svoju efikasnost da bi ispunili uslove povećane potražnje ovih proizvoda uz prilagođavanje životinjskih genetičkih resursa promenljivim ekonomskim i ekološkim uslovima. Prema podacima FAO (2007a), značajan deo od oko
7.000 rasa stoke u svetu je u opasnosti od izumiranja, a veliki problem predstavlja i što mnoge zemlje i dalje nemaju tehnički i materijalni kapacitet da
obezbede pravilno upravljanje i održivost njihovih genetičkih resursa. Očuvanje
životinjskih genetičkih resursa je treća strateška prioritetna oblast u svetu u
okviru globalnog akcionog plana. Čuvanje genetičkih resursa uključuje i održavanje in vivo i upravljanje genetičkom raznolikoću unutar populacija životinja.
Razvoj banke gena koja omogućava kriokonzervaciju gena poreklom od
životinja zahteva tehničke i naučne kapacitete u genetici, reproduktivnoj fiziologiji, kriobiologiji i bioinformatici. Strategije očuvanja genetičkih resursa
mogu se kategorisati kao in situ konzervacija (u kojoj se životinje održavaju u
okruženju ili u proizvodnim sistemima u kojima su razvijeni) ili kao ex situ
konzervacija (svi ostali slučajevi). Drugo mogu se dalje podeliti na ex situ – in
vivo i in vitro konzervaciju i kriokonzervaciju. Kriokonzervacija in vitro podrazumeva čuvanje uzoraka duboko zamrznutog semena, jajnih ćelija, embriona
ili tkiva za potencijalnu buduću upotrebu u uzgoju.
PB  - Beograd : Fakultet veterinarske medicine
C3  - Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023
T1  - Mogućnost krioprezervacije reproduktivnog materijala autohtonih vrsta domaćih životinja u očuvanju animalnih genetičkih resursa
SP  - 75
EP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3019
ER  - 

@conference{
author = "Vakanjac, Slobodanka and Nedić, Svetlana and Magaš, Vladimir and Blagojević, Jovan and Maletić, Milan",
year = "2023",
abstract = "Veliki ekonomski razvoj, rast i mobilnost stanovništva, povećali su potražnju za
mlečnim i mesnim proizvodima, ali su takođe izvršili pritisak na održivost ruralnih sredina i menadžment proizvodnje hrane. Uzgajivači stoke bi trebalo da
povećaju svoju efikasnost da bi ispunili uslove povećane potražnje ovih proizvoda uz prilagođavanje životinjskih genetičkih resursa promenljivim ekonomskim i ekološkim uslovima. Prema podacima FAO (2007a), značajan deo od oko
7.000 rasa stoke u svetu je u opasnosti od izumiranja, a veliki problem predstavlja i što mnoge zemlje i dalje nemaju tehnički i materijalni kapacitet da
obezbede pravilno upravljanje i održivost njihovih genetičkih resursa. Očuvanje
životinjskih genetičkih resursa je treća strateška prioritetna oblast u svetu u
okviru globalnog akcionog plana. Čuvanje genetičkih resursa uključuje i održavanje in vivo i upravljanje genetičkom raznolikoću unutar populacija životinja.
Razvoj banke gena koja omogućava kriokonzervaciju gena poreklom od
životinja zahteva tehničke i naučne kapacitete u genetici, reproduktivnoj fiziologiji, kriobiologiji i bioinformatici. Strategije očuvanja genetičkih resursa
mogu se kategorisati kao in situ konzervacija (u kojoj se životinje održavaju u
okruženju ili u proizvodnim sistemima u kojima su razvijeni) ili kao ex situ
konzervacija (svi ostali slučajevi). Drugo mogu se dalje podeliti na ex situ – in
vivo i in vitro konzervaciju i kriokonzervaciju. Kriokonzervacija in vitro podrazumeva čuvanje uzoraka duboko zamrznutog semena, jajnih ćelija, embriona
ili tkiva za potencijalnu buduću upotrebu u uzgoju.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023",
title = "Mogućnost krioprezervacije reproduktivnog materijala autohtonih vrsta domaćih životinja u očuvanju animalnih genetičkih resursa",
pages = "75-84",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3019"
}

Vakanjac, S., Nedić, S., Magaš, V., Blagojević, J.,& Maletić, M.. (2023). Mogućnost krioprezervacije reproduktivnog materijala autohtonih vrsta domaćih životinja u očuvanju animalnih genetičkih resursa. in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023
Beograd : Fakultet veterinarske medicine., 75-84.
https://hdl.handle.net/21.15107/rcub_veterinar_3019

Vakanjac S, Nedić S, Magaš V, Blagojević J, Maletić M. Mogućnost krioprezervacije reproduktivnog materijala autohtonih vrsta domaćih životinja u očuvanju animalnih genetičkih resursa. in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023. 2023;:75-84.
https://hdl.handle.net/21.15107/rcub_veterinar_3019 .

Vakanjac, Slobodanka, Nedić, Svetlana, Magaš, Vladimir, Blagojević, Jovan, Maletić, Milan, "Mogućnost krioprezervacije reproduktivnog materijala autohtonih vrsta domaćih životinja u očuvanju animalnih genetičkih resursa" in Četvrti regionalni simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 29. jun - 1. jul 2023 (2023):75-84,
https://hdl.handle.net/21.15107/rcub_veterinar_3019 .

TY  - CONF
AU  - Vakanjac, Slobodanka
AU  - Stanišić, Ljubodrag
AU  - Magaš, Vladimir
AU  - Đurić, Miloje
AU  - Arsić, Sveta
AU  - Maletić, Milan
AU  - Nedić, Svetlana
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3284
AB  - Glavni cilj svih biotehnologija u reprodukciji je postizanje uspešnog graviditeta.
Ejakulati nerastova koji se koriste za veštačko osemenjavanje krmača, obično
se selektuju na osnovu parametara pokretljivosti koji se procenjuju upotrebom
inovativnih tehnika kao što je kompjuterski asistirana analiza semena – CASA.
Iako nove tehnike daju mnogo objektivnije i detaljnije analize pokretljivosti spermatozoida,
povezanost dobijenih parametara u oceni kvaliteta semena sa in vivo
fertilitetom još uvek je diskutabilna. Stoga, analiza komponenti seminalne plazme
može biti od velikog kliničkog značaja u ispitivanju fertilnosti ili steriliteta nerastova,
kao i ranom otkrivanju subfertilnih nerastova. Seminalna plazma je složena
smeša egzokrinih sekreta testisa, epididimisa i akcesornih polnih žlezda mužjaka
u kojoj se nalazi širok spektar različitih organskih i neorganskih komponenti. Ispitivanje
biohemijskog sastava seminalne plazme od velikog je značaja jer može
ukazati na normalnu ili promenjenu funkciju i/ili oštećenje spermatozoida.
Cilj rada bio je da se ispita povezanost pojedinih biohemijskih parametara
i ukupnog antioksidativnog kapaciteta seminalne plazme sa parametrima pokretljivosti
i kinetike spermatozoida, kao i mogućnost upotrebe nekog od parametara
kao mogućeg biomarkera kvaliteta semena. U radu je kompjuterski asistiranom
analizom semena (CASA) i citomorfološkim pregledom ispitan kvalitet, odnosno
pokretljivost i kinetika spermatozoida od 39 nerastova. U seminalnoj plazmi ispitivane
su koncentracije Ca, Na, K, Cl, P, Mg, laktata, ukupnih proteina, albumina,
holesterola, aktivnost enzima alkalne-fosfataze, kreatin-kinaze i laktat-dehidrogenaze
kao i ukupan antioksidativni kapacitet. Utvrđena je negativna korelacija procenta
ukupno i progresivno pokretnih spermatozoida sa P, aktivnošću alkalne-fosfataze
i pozitivna korelacija sa ukupnim holesterolom, aktivnošću enzima kreatin-kinaze i ukupnim antioksidativnim kapacitetom. Procenat brzih spermatozoida
bio je u pozitivnoj korelaciji sa koncentracijom Cl, laktata i laktat-dehidrogenaze, i
u negativnoj korelaciji sa P, Mg i aktivnošću alkalne-fosfataze. Utvrđena je negativna
korelacija parametara kinetike spermatozoida: VCL, VAP, DCL, DAP, ALH,
BCF i HAC i procenatualne zastupljenosti morfološki abnormalnih spermatozoida
u semenu. Takođe, aktivnost enzima laktat-dehidrogenaze bila je u pozitivnoj korelaciji
sa DCL i ALH. Rezultati ovog rada ukazuju na povezanost biohemijskog
sastava seminalne plazme sa pokretljivošću i kinetikom spermatozoida. Određivanje
aktivnosti laktat-dehidrogenaze bi se moglo preporučiti pri rutinskoj oceni
kvaliteta semena nerastova.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - 14. Naučni simpozijum Reprodukcija životinja, Divčibare, 12 - 15. oktobar 2023
T1  - Korelaciona povezanost sastava seminalne plazme nerastova sa pokretljivošću i kinetikom spermatozoida
SP  - 105
EP  - 114
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3284
ER  - 

@conference{
author = "Vakanjac, Slobodanka and Stanišić, Ljubodrag and Magaš, Vladimir and Đurić, Miloje and Arsić, Sveta and Maletić, Milan and Nedić, Svetlana",
year = "2023",
abstract = "Glavni cilj svih biotehnologija u reprodukciji je postizanje uspešnog graviditeta.
Ejakulati nerastova koji se koriste za veštačko osemenjavanje krmača, obično
se selektuju na osnovu parametara pokretljivosti koji se procenjuju upotrebom
inovativnih tehnika kao što je kompjuterski asistirana analiza semena – CASA.
Iako nove tehnike daju mnogo objektivnije i detaljnije analize pokretljivosti spermatozoida,
povezanost dobijenih parametara u oceni kvaliteta semena sa in vivo
fertilitetom još uvek je diskutabilna. Stoga, analiza komponenti seminalne plazme
može biti od velikog kliničkog značaja u ispitivanju fertilnosti ili steriliteta nerastova,
kao i ranom otkrivanju subfertilnih nerastova. Seminalna plazma je složena
smeša egzokrinih sekreta testisa, epididimisa i akcesornih polnih žlezda mužjaka
u kojoj se nalazi širok spektar različitih organskih i neorganskih komponenti. Ispitivanje
biohemijskog sastava seminalne plazme od velikog je značaja jer može
ukazati na normalnu ili promenjenu funkciju i/ili oštećenje spermatozoida.
Cilj rada bio je da se ispita povezanost pojedinih biohemijskih parametara
i ukupnog antioksidativnog kapaciteta seminalne plazme sa parametrima pokretljivosti
i kinetike spermatozoida, kao i mogućnost upotrebe nekog od parametara
kao mogućeg biomarkera kvaliteta semena. U radu je kompjuterski asistiranom
analizom semena (CASA) i citomorfološkim pregledom ispitan kvalitet, odnosno
pokretljivost i kinetika spermatozoida od 39 nerastova. U seminalnoj plazmi ispitivane
su koncentracije Ca, Na, K, Cl, P, Mg, laktata, ukupnih proteina, albumina,
holesterola, aktivnost enzima alkalne-fosfataze, kreatin-kinaze i laktat-dehidrogenaze
kao i ukupan antioksidativni kapacitet. Utvrđena je negativna korelacija procenta
ukupno i progresivno pokretnih spermatozoida sa P, aktivnošću alkalne-fosfataze
i pozitivna korelacija sa ukupnim holesterolom, aktivnošću enzima kreatin-kinaze i ukupnim antioksidativnim kapacitetom. Procenat brzih spermatozoida
bio je u pozitivnoj korelaciji sa koncentracijom Cl, laktata i laktat-dehidrogenaze, i
u negativnoj korelaciji sa P, Mg i aktivnošću alkalne-fosfataze. Utvrđena je negativna
korelacija parametara kinetike spermatozoida: VCL, VAP, DCL, DAP, ALH,
BCF i HAC i procenatualne zastupljenosti morfološki abnormalnih spermatozoida
u semenu. Takođe, aktivnost enzima laktat-dehidrogenaze bila je u pozitivnoj korelaciji
sa DCL i ALH. Rezultati ovog rada ukazuju na povezanost biohemijskog
sastava seminalne plazme sa pokretljivošću i kinetikom spermatozoida. Određivanje
aktivnosti laktat-dehidrogenaze bi se moglo preporučiti pri rutinskoj oceni
kvaliteta semena nerastova.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "14. Naučni simpozijum Reprodukcija životinja, Divčibare, 12 - 15. oktobar 2023",
title = "Korelaciona povezanost sastava seminalne plazme nerastova sa pokretljivošću i kinetikom spermatozoida",
pages = "105-114",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3284"
}

Vakanjac, S., Stanišić, L., Magaš, V., Đurić, M., Arsić, S., Maletić, M.,& Nedić, S.. (2023). Korelaciona povezanost sastava seminalne plazme nerastova sa pokretljivošću i kinetikom spermatozoida. in 14. Naučni simpozijum Reprodukcija životinja, Divčibare, 12 - 15. oktobar 2023
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 105-114.
https://hdl.handle.net/21.15107/rcub_veterinar_3284

Vakanjac S, Stanišić L, Magaš V, Đurić M, Arsić S, Maletić M, Nedić S. Korelaciona povezanost sastava seminalne plazme nerastova sa pokretljivošću i kinetikom spermatozoida. in 14. Naučni simpozijum Reprodukcija životinja, Divčibare, 12 - 15. oktobar 2023. 2023;:105-114.
https://hdl.handle.net/21.15107/rcub_veterinar_3284 .

Vakanjac, Slobodanka, Stanišić, Ljubodrag, Magaš, Vladimir, Đurić, Miloje, Arsić, Sveta, Maletić, Milan, Nedić, Svetlana, "Korelaciona povezanost sastava seminalne plazme nerastova sa pokretljivošću i kinetikom spermatozoida" in 14. Naučni simpozijum Reprodukcija životinja, Divčibare, 12 - 15. oktobar 2023 (2023):105-114,
https://hdl.handle.net/21.15107/rcub_veterinar_3284 .

TY  - CONF
AU  - Stanišić, Ljubodrag
AU  - Magaš, Vladimir
AU  - Nedić, Svetlana
AU  - Maletić, Milan
AU  - Vakanjac, Slobodanka
AU  - Nestorović, Ivan
AU  - Đurić, Miloje
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3306
AB  - Veštačko osemenjavanje predstavlja manuelno deponovanje sperme ili
semena u reproduktivni trakt ženki različitim metodama koje nisu prirodno
parenje. Veštačko osemenjavanje je jedna iz grupe tehnika opšte poznatih kao
„asistirane reproduktivne tehnike” (ART), kojima se omogućava spajanje
gameta (spermatozoida i oocita). Glavne prednosti veštačkog osemenjavanja
su: sprečavanje širenja uzročnika bolesti, povećanje stope ispoljavanja
genetičkog potencijala jedniki, omogućavanje parenja jedinki različitih
geografskih lokacija i u različito vreme (nakon smrti), omogućavanje parenja
u slučaju nastanka fizičkih, fizioloških i bihejvioralnih poremećaja, očuvanje
retkih i ugroženih vrsta, i kao nezaobilazna tehnika kod programa
krioprezervacije i seksiranja semena.
Uspešnost veštačkog osemenjavanja zavisi od mnogo faktora, a
preduslov je dobro poznavanje specifičnosti ciklusa kuje ali i fiziologije
sperme mužijaka i načina ejakulacije. Sa jedne strane, veoma je važna
procena odgovarajuće faze ciklusa kuje i idealnog vremena za osemenjavanje,
dok je sa druge strane neophodno pravilno uzorkovati ejakulat psa, analizirati
sve tri frakcije ejakulata i na osnovu kompletne analize proceniti kvalitet
sperme. Veterinar, na osnovu dobijenih podataka vezanih za kuju i mužijaka,
ali i na osnovu iskustva, obučenosti, opremljenosti, uz dobro razumevanje stope uspešnosti,
odlučuje se za odgovarajuću tehniku osemenjavanja. Opisano je pet oblika
osemenjavanja kod kuja: intravaginalna deponovanje semena, transcervikalno
osemenjavanje uz pomoć endoskopa, transcervikalno osemenjavanje uz
pomoć norveškog katetera, laparotomska (hirurška) inseminacija i
laparoskopska inseminacija.
PB  - Beograd : Fakultet veterinarske medicine
C3  - 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 -10. jun 2023
T1  - Veštačko osemenjavanje kuja – male tajne i velike zablude
SP  - 24
EP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3306
ER  - 

@conference{
author = "Stanišić, Ljubodrag and Magaš, Vladimir and Nedić, Svetlana and Maletić, Milan and Vakanjac, Slobodanka and Nestorović, Ivan and Đurić, Miloje",
year = "2023",
abstract = "Veštačko osemenjavanje predstavlja manuelno deponovanje sperme ili
semena u reproduktivni trakt ženki različitim metodama koje nisu prirodno
parenje. Veštačko osemenjavanje je jedna iz grupe tehnika opšte poznatih kao
„asistirane reproduktivne tehnike” (ART), kojima se omogućava spajanje
gameta (spermatozoida i oocita). Glavne prednosti veštačkog osemenjavanja
su: sprečavanje širenja uzročnika bolesti, povećanje stope ispoljavanja
genetičkog potencijala jedniki, omogućavanje parenja jedinki različitih
geografskih lokacija i u različito vreme (nakon smrti), omogućavanje parenja
u slučaju nastanka fizičkih, fizioloških i bihejvioralnih poremećaja, očuvanje
retkih i ugroženih vrsta, i kao nezaobilazna tehnika kod programa
krioprezervacije i seksiranja semena.
Uspešnost veštačkog osemenjavanja zavisi od mnogo faktora, a
preduslov je dobro poznavanje specifičnosti ciklusa kuje ali i fiziologije
sperme mužijaka i načina ejakulacije. Sa jedne strane, veoma je važna
procena odgovarajuće faze ciklusa kuje i idealnog vremena za osemenjavanje,
dok je sa druge strane neophodno pravilno uzorkovati ejakulat psa, analizirati
sve tri frakcije ejakulata i na osnovu kompletne analize proceniti kvalitet
sperme. Veterinar, na osnovu dobijenih podataka vezanih za kuju i mužijaka,
ali i na osnovu iskustva, obučenosti, opremljenosti, uz dobro razumevanje stope uspešnosti,
odlučuje se za odgovarajuću tehniku osemenjavanja. Opisano je pet oblika
osemenjavanja kod kuja: intravaginalna deponovanje semena, transcervikalno
osemenjavanje uz pomoć endoskopa, transcervikalno osemenjavanje uz
pomoć norveškog katetera, laparotomska (hirurška) inseminacija i
laparoskopska inseminacija.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 -10. jun 2023",
title = "Veštačko osemenjavanje kuja – male tajne i velike zablude",
pages = "24-25",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3306"
}

Stanišić, L., Magaš, V., Nedić, S., Maletić, M., Vakanjac, S., Nestorović, I.,& Đurić, M.. (2023). Veštačko osemenjavanje kuja – male tajne i velike zablude. in 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 -10. jun 2023
Beograd : Fakultet veterinarske medicine., 24-25.
https://hdl.handle.net/21.15107/rcub_veterinar_3306

Stanišić L, Magaš V, Nedić S, Maletić M, Vakanjac S, Nestorović I, Đurić M. Veštačko osemenjavanje kuja – male tajne i velike zablude. in 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 -10. jun 2023. 2023;:24-25.
https://hdl.handle.net/21.15107/rcub_veterinar_3306 .

Stanišić, Ljubodrag, Magaš, Vladimir, Nedić, Svetlana, Maletić, Milan, Vakanjac, Slobodanka, Nestorović, Ivan, Đurić, Miloje, "Veštačko osemenjavanje kuja – male tajne i velike zablude" in 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 -10. jun 2023 (2023):24-25,
https://hdl.handle.net/21.15107/rcub_veterinar_3306 .

TY  - CONF
AU  - Magaš, Vladimir
AU  - Stanišić, Ljubodrag
AU  - Nedić, Svetlana
AU  - Maletić, Milan
AU  - Vakanjac, Slobodanka
AU  - Nestorović, Ivan
AU  - Pavlović, Miloš
AU  - Đurić, Miloje
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3309
AB  - Carski rez kod kuje je rutinska porodiljska tehnika sa visokim procentom
preživljavanja i majke i ploda(ova). Ovaj zahvat je daleko brži i sigurniji u
slučajevima distokije kod kuja, a istovremeno i manje naporan po samu porodilju
ali i vlasnika. Najznačajniji korak u samom izvođenju ove operativne tehnike
jeste pravovremena i tačna dijagnoza potencijalnih uzroka teškog porođaja, a sa
tim u vezi i blagovremeno započinjanje samog carskog reza. Dobra prognoza
nakon završetka carskog reza može se očekivati ukoliko su ispunjeni neki od
sledećih uslova: pravilna dijagnostika za pravovremeno izvođenje, spretnost i
brzina samog hirurga, dostupnost asistenata, odgovarajući uslovi u kom se izvodi
sam zahvat, prisutnost odnosno izostanak određenih sistemskih i metaboličkih
oboljenja porodilje, telesna kondicija, vitalnost ploda(ova), itd. Indikacije za
carski rez su višestruke, a neke od najvažnijih apsolutnih indikacija su atonija
uterusa (primarna i sekundarna), nepotpuno otvaranje cerviksa, emfizem ploda,
povrede mekog i koštanog dela porođajnog kanala, mali broj plodova, torzija
uterusa porodilje, nakaze, nespremnost vlasnika, itd. Definisanje pravilnog
pristupa i tehnike dijagnostike, anestezije, samog izvođenja carskog reza i
postoperativan tretman štenaca i porodilje je od suštinske važnosti za uspešan
ishod.
PB  - Beograd : Fakultet veterinarske medicine
C3  - 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023
T1  - Carski rez kod kuja – u pravo vreme i na pravi način
SP  - 28
EP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3309
ER  - 

@conference{
author = "Magaš, Vladimir and Stanišić, Ljubodrag and Nedić, Svetlana and Maletić, Milan and Vakanjac, Slobodanka and Nestorović, Ivan and Pavlović, Miloš and Đurić, Miloje",
year = "2023",
abstract = "Carski rez kod kuje je rutinska porodiljska tehnika sa visokim procentom
preživljavanja i majke i ploda(ova). Ovaj zahvat je daleko brži i sigurniji u
slučajevima distokije kod kuja, a istovremeno i manje naporan po samu porodilju
ali i vlasnika. Najznačajniji korak u samom izvođenju ove operativne tehnike
jeste pravovremena i tačna dijagnoza potencijalnih uzroka teškog porođaja, a sa
tim u vezi i blagovremeno započinjanje samog carskog reza. Dobra prognoza
nakon završetka carskog reza može se očekivati ukoliko su ispunjeni neki od
sledećih uslova: pravilna dijagnostika za pravovremeno izvođenje, spretnost i
brzina samog hirurga, dostupnost asistenata, odgovarajući uslovi u kom se izvodi
sam zahvat, prisutnost odnosno izostanak određenih sistemskih i metaboličkih
oboljenja porodilje, telesna kondicija, vitalnost ploda(ova), itd. Indikacije za
carski rez su višestruke, a neke od najvažnijih apsolutnih indikacija su atonija
uterusa (primarna i sekundarna), nepotpuno otvaranje cerviksa, emfizem ploda,
povrede mekog i koštanog dela porođajnog kanala, mali broj plodova, torzija
uterusa porodilje, nakaze, nespremnost vlasnika, itd. Definisanje pravilnog
pristupa i tehnike dijagnostike, anestezije, samog izvođenja carskog reza i
postoperativan tretman štenaca i porodilje je od suštinske važnosti za uspešan
ishod.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023",
title = "Carski rez kod kuja – u pravo vreme i na pravi način",
pages = "28-28",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3309"
}

Magaš, V., Stanišić, L., Nedić, S., Maletić, M., Vakanjac, S., Nestorović, I., Pavlović, M.,& Đurić, M.. (2023). Carski rez kod kuja – u pravo vreme i na pravi način. in 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023
Beograd : Fakultet veterinarske medicine., 28-28.
https://hdl.handle.net/21.15107/rcub_veterinar_3309

Magaš V, Stanišić L, Nedić S, Maletić M, Vakanjac S, Nestorović I, Pavlović M, Đurić M. Carski rez kod kuja – u pravo vreme i na pravi način. in 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023. 2023;:28-28.
https://hdl.handle.net/21.15107/rcub_veterinar_3309 .

Magaš, Vladimir, Stanišić, Ljubodrag, Nedić, Svetlana, Maletić, Milan, Vakanjac, Slobodanka, Nestorović, Ivan, Pavlović, Miloš, Đurić, Miloje, "Carski rez kod kuja – u pravo vreme i na pravi način" in 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023 (2023):28-28,
https://hdl.handle.net/21.15107/rcub_veterinar_3309 .

TY  - CONF
AU  - Vakanjac, Slobodanka
AU  - Maletić, Milan
AU  - Đurić, Miloje
AU  - Magaš, Vladimir
AU  - Stanišić, Ljubodrag
AU  - Nedić, Svetlana
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3612
AB  - Bacterial contamination of boar semen occurs with some frequency in artificial insemination centers and may have a negative effect on the quality of the semen as well as on the sows’ reproductive capacity. In this case report, the boar was clinically healthy, but on farms there were registered reproductive failures. The study focused on the identification of gram-negative bacteria Klebsiella oxytoca (K. oxytoca) in boar semen, and its impact on the quality of ejaculates obtained from boar. The classification of bacteria into Klebsiella genus was confirmed by commercial biochemical tests, Microgen GNA System (Camberley, UK). The numbers of K. oxytoca colonies were determined in diluted boar semen samples by streaking each on the surface of nonselective Nutrition agar (LabM, UK). The semen samples were contaminated with K. oxytoca above 2 × 104 colony-forming units/mL (CFU/mL) at first semen analyses and 4.8 x 105 CFU/mL after 14 days. Klebsiella oxytoca is a gram-negative microbe generally associated with the community. This bacteria is an opportunistic pathogen implicated in various clinical diseases in animals and humans. Various motility parameters as well as sperm concentration were analyzed using the CASA system (Minitube, AndroVision, Tiefenbach, Germany), on 0 days and after 14 days. Total motility in boar semen was lower by 3.21 fold in boar semen with a larger number of K. oxytoca than in those with a smaller number of bacteria. Also, progressive motility was lower by 2.83 fold, the percentage of fast spermatozoa was lower by 2.63 fold in semen with a larger number of bacteria. The degree of bacterial contamination in ejaculates directly influences sperm quality parameters. Thus, on the basis of the pathological effects that K. oxytoca may have on boar sperm quality, bacterial contamination should always be examined in semen samples prepared for artificial insemination.
PB  - Novi Sad : Faculty of Agriculture
C3  - International Symposium on Animal Science (ISAS), Novi Sad, 18 - 20. September 2023
T1  - The impact of Klepsiella oxytoca on boar sperm quality
SP  - 2
EP  - 2
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3612
ER  - 

@conference{
author = "Vakanjac, Slobodanka and Maletić, Milan and Đurić, Miloje and Magaš, Vladimir and Stanišić, Ljubodrag and Nedić, Svetlana",
year = "2023",
abstract = "Bacterial contamination of boar semen occurs with some frequency in artificial insemination centers and may have a negative effect on the quality of the semen as well as on the sows’ reproductive capacity. In this case report, the boar was clinically healthy, but on farms there were registered reproductive failures. The study focused on the identification of gram-negative bacteria Klebsiella oxytoca (K. oxytoca) in boar semen, and its impact on the quality of ejaculates obtained from boar. The classification of bacteria into Klebsiella genus was confirmed by commercial biochemical tests, Microgen GNA System (Camberley, UK). The numbers of K. oxytoca colonies were determined in diluted boar semen samples by streaking each on the surface of nonselective Nutrition agar (LabM, UK). The semen samples were contaminated with K. oxytoca above 2 × 104 colony-forming units/mL (CFU/mL) at first semen analyses and 4.8 x 105 CFU/mL after 14 days. Klebsiella oxytoca is a gram-negative microbe generally associated with the community. This bacteria is an opportunistic pathogen implicated in various clinical diseases in animals and humans. Various motility parameters as well as sperm concentration were analyzed using the CASA system (Minitube, AndroVision, Tiefenbach, Germany), on 0 days and after 14 days. Total motility in boar semen was lower by 3.21 fold in boar semen with a larger number of K. oxytoca than in those with a smaller number of bacteria. Also, progressive motility was lower by 2.83 fold, the percentage of fast spermatozoa was lower by 2.63 fold in semen with a larger number of bacteria. The degree of bacterial contamination in ejaculates directly influences sperm quality parameters. Thus, on the basis of the pathological effects that K. oxytoca may have on boar sperm quality, bacterial contamination should always be examined in semen samples prepared for artificial insemination.",
publisher = "Novi Sad : Faculty of Agriculture",
journal = "International Symposium on Animal Science (ISAS), Novi Sad, 18 - 20. September 2023",
title = "The impact of Klepsiella oxytoca on boar sperm quality",
pages = "2-2",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3612"
}

Vakanjac, S., Maletić, M., Đurić, M., Magaš, V., Stanišić, L.,& Nedić, S.. (2023). The impact of Klepsiella oxytoca on boar sperm quality. in International Symposium on Animal Science (ISAS), Novi Sad, 18 - 20. September 2023
Novi Sad : Faculty of Agriculture., 2-2.
https://hdl.handle.net/21.15107/rcub_veterinar_3612

Vakanjac S, Maletić M, Đurić M, Magaš V, Stanišić L, Nedić S. The impact of Klepsiella oxytoca on boar sperm quality. in International Symposium on Animal Science (ISAS), Novi Sad, 18 - 20. September 2023. 2023;:2-2.
https://hdl.handle.net/21.15107/rcub_veterinar_3612 .

Vakanjac, Slobodanka, Maletić, Milan, Đurić, Miloje, Magaš, Vladimir, Stanišić, Ljubodrag, Nedić, Svetlana, "The impact of Klepsiella oxytoca on boar sperm quality" in International Symposium on Animal Science (ISAS), Novi Sad, 18 - 20. September 2023 (2023):2-2,
https://hdl.handle.net/21.15107/rcub_veterinar_3612 .

TY  - JOUR
AU  - Nedić, Svetlana
AU  - Đurić, Miloje
AU  - Vakanjac, Slobodanka
AU  - Arsić, Sveta
AU  - Nedić, Sreten
AU  - Samardžija, Marko
AU  - Borozan, Sunčica
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2523
AB  - The aim of this study was to examine the biochemical components and the parameters of antioxidant protection in the
seminal plasma (SP) of boars, as well as their relationship with semen quality parameters. Thirty-six boars were included
in the study, whose ejaculates were divided into two groups: Group I (good quality semen, > 70% progressively motile
sperm, < 20% spermatozoa with abnormal morphology, n=16), and Group II (poor quality semen, < 70% progressively
motile sperm, > 20% spermatozoa with abnormal morphology, n=20). Significantly higher concentrations of ionized calcium (iCa), total cholesterol (TC), lactate-dehydrogenase (LDH) activity, as well as significantly higher values of antioxidant protection parameters: thiol groups (-SH), paraoxonase 1 (PON1), and total antioxidant capacity (TAC) ) were found
in the good quality semen, while higher phosphorus (P) concentrations and increased alkaline-phosphatase (ALP) activity
were found in the semen of poor quality. A negative correlation of total and progressive sperm motility with P and ALP
was found in all examined semen samples, while a positive correlation was found with PON1 and TAC. The percentage
of fast sperm cells positively correlated with iCa, chlorine (Cl), lactate, LDH and TAC, while a negative correlation was
found with P, magnesium (Mg) and the enzyme creatine-kinase (CK). The percentage of immobile sperm positively correlated with P and ALP, and negatively correlated with TC, CK, PON1 and TAC. Elevated values of PON1 and TAC in
SP and a positive correlation with sperm motility indicate the possible use of these parameters as sensitive biomarkers of
boar semen quality. To the best of our knowledge there are no published data on association between PON1 activity and
boar semen quality.
PB  - Springer
T2  - Veterinary Research Communications
T1  - Relationship between biochemical parameters and paraoxonase 1 activity of boar seminal plasma and semen quality
VL  - 47
SP  - 1243
EP  - 1253
DO  - 10.1007/s11259-022-10066-x
ER  - 

@article{
author = "Nedić, Svetlana and Đurić, Miloje and Vakanjac, Slobodanka and Arsić, Sveta and Nedić, Sreten and Samardžija, Marko and Borozan, Sunčica",
year = "2023",
abstract = "The aim of this study was to examine the biochemical components and the parameters of antioxidant protection in the
seminal plasma (SP) of boars, as well as their relationship with semen quality parameters. Thirty-six boars were included
in the study, whose ejaculates were divided into two groups: Group I (good quality semen, > 70% progressively motile
sperm, < 20% spermatozoa with abnormal morphology, n=16), and Group II (poor quality semen, < 70% progressively
motile sperm, > 20% spermatozoa with abnormal morphology, n=20). Significantly higher concentrations of ionized calcium (iCa), total cholesterol (TC), lactate-dehydrogenase (LDH) activity, as well as significantly higher values of antioxidant protection parameters: thiol groups (-SH), paraoxonase 1 (PON1), and total antioxidant capacity (TAC) ) were found
in the good quality semen, while higher phosphorus (P) concentrations and increased alkaline-phosphatase (ALP) activity
were found in the semen of poor quality. A negative correlation of total and progressive sperm motility with P and ALP
was found in all examined semen samples, while a positive correlation was found with PON1 and TAC. The percentage
of fast sperm cells positively correlated with iCa, chlorine (Cl), lactate, LDH and TAC, while a negative correlation was
found with P, magnesium (Mg) and the enzyme creatine-kinase (CK). The percentage of immobile sperm positively correlated with P and ALP, and negatively correlated with TC, CK, PON1 and TAC. Elevated values of PON1 and TAC in
SP and a positive correlation with sperm motility indicate the possible use of these parameters as sensitive biomarkers of
boar semen quality. To the best of our knowledge there are no published data on association between PON1 activity and
boar semen quality.",
publisher = "Springer",
journal = "Veterinary Research Communications",
title = "Relationship between biochemical parameters and paraoxonase 1 activity of boar seminal plasma and semen quality",
volume = "47",
pages = "1243-1253",
doi = "10.1007/s11259-022-10066-x"
}

Nedić, S., Đurić, M., Vakanjac, S., Arsić, S., Nedić, S., Samardžija, M.,& Borozan, S.. (2023). Relationship between biochemical parameters and paraoxonase 1 activity of boar seminal plasma and semen quality. in Veterinary Research Communications
Springer., 47, 1243-1253.
https://doi.org/10.1007/s11259-022-10066-x

Nedić S, Đurić M, Vakanjac S, Arsić S, Nedić S, Samardžija M, Borozan S. Relationship between biochemical parameters and paraoxonase 1 activity of boar seminal plasma and semen quality. in Veterinary Research Communications. 2023;47:1243-1253.
doi:10.1007/s11259-022-10066-x .

Nedić, Svetlana, Đurić, Miloje, Vakanjac, Slobodanka, Arsić, Sveta, Nedić, Sreten, Samardžija, Marko, Borozan, Sunčica, "Relationship between biochemical parameters and paraoxonase 1 activity of boar seminal plasma and semen quality" in Veterinary Research Communications, 47 (2023):1243-1253,
https://doi.org/10.1007/s11259-022-10066-x . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Nedić, Sreten
AU  - Vujanac, Ivan
AU  - Bojkovski, Jovan
AU  - Nedić, Svetlana
AU  - Jovanović, Ljubomir
AU  - Kirovski, Danijela
AU  - Borozan, Sunčica
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2860
AB  - Cows in the peripartal period undergo changes in thyroid hormones and are susceptible to lipomobilization and/or oxidative stress. The addition of chestnut tannins as polyphenolic compounds in the diet may improve feed efficiency and prevent oxidative stress-related health disorders in transition cows. However, the relationship between chestnut tannin supplementation and thyroid function, which plays an important role in metabolic regulation, has not been investigated in dairy cows. This study was conducted to investigate the effects of chestnut tannin supplementation during the close-up period on thyroid status and to evaluate the interaction between thyroid hormones and oxidative stress biomarkers in prepartum dairy cows. Forty multiparous Holstein cows were fed either a diet containing chestnut tannins (CNTs, n = 20, 1.96 g chestnut tannins/kg feed, dry matter) or a non-supplemented diet (CON, n = 20) during the last 25 ± 2 days of gestation. Blood samples were collected on the first day of study (before chestnut tannin supplementation) and d 5 before parturition to measure hormonal and oxidative stress indices. Serum concentrations of T3 (p = 0.04) and T4 (p = 0.05) were higher in CNT cows than in the CON group on day 5 before parturition. Thyroid status of CNT cows was associated with higher serum total antioxidant capacity (T-AOC, p < 0.01), activities of superoxide dismutase (SOD, p = 0.03) and glutathione peroxidase (GPx, p = 0.01), and reduced glutathione concentration (GSH, p = 0.05). Serum thiobarbituric acid reactive substances (TBARS) were lower (p = 0.04) which was associated with lower aspartate aminotransferase (AST, p = 0.02), and lactate dehydrogenase (LDH, p = 0.01) activities in the CNT than in the CON group. Estradiol and progesterone did not differ between CNT and CON cows. Chestnut tannin supplementation improves antioxidant protection, prevents oxidation-reduction processes, reduces the degree of liver cell membrane damage, and protects thyroid tissue from damage, allowing higher T3 and T4 synthesis. Considering the importance of the thyroid hormone status before parturition, mechanisms of thyroid hormone regulation in CNT-supplemented dairy cows require more detailed investigations.
PB  - MDPI
T2  - Metabolites
T1  - Dietary Supplementation of Chestnut Tannins in Prepartum Dairy Cows Improves Antioxidant Defense Mechanisms Interacting with Thyroid Status
VL  - 13
IS  - 3
SP  - 334
DO  - 10.3390/metabo13030334
ER  - 

@article{
author = "Prodanović, Radiša and Nedić, Sreten and Vujanac, Ivan and Bojkovski, Jovan and Nedić, Svetlana and Jovanović, Ljubomir and Kirovski, Danijela and Borozan, Sunčica",
year = "2023",
abstract = "Cows in the peripartal period undergo changes in thyroid hormones and are susceptible to lipomobilization and/or oxidative stress. The addition of chestnut tannins as polyphenolic compounds in the diet may improve feed efficiency and prevent oxidative stress-related health disorders in transition cows. However, the relationship between chestnut tannin supplementation and thyroid function, which plays an important role in metabolic regulation, has not been investigated in dairy cows. This study was conducted to investigate the effects of chestnut tannin supplementation during the close-up period on thyroid status and to evaluate the interaction between thyroid hormones and oxidative stress biomarkers in prepartum dairy cows. Forty multiparous Holstein cows were fed either a diet containing chestnut tannins (CNTs, n = 20, 1.96 g chestnut tannins/kg feed, dry matter) or a non-supplemented diet (CON, n = 20) during the last 25 ± 2 days of gestation. Blood samples were collected on the first day of study (before chestnut tannin supplementation) and d 5 before parturition to measure hormonal and oxidative stress indices. Serum concentrations of T3 (p = 0.04) and T4 (p = 0.05) were higher in CNT cows than in the CON group on day 5 before parturition. Thyroid status of CNT cows was associated with higher serum total antioxidant capacity (T-AOC, p < 0.01), activities of superoxide dismutase (SOD, p = 0.03) and glutathione peroxidase (GPx, p = 0.01), and reduced glutathione concentration (GSH, p = 0.05). Serum thiobarbituric acid reactive substances (TBARS) were lower (p = 0.04) which was associated with lower aspartate aminotransferase (AST, p = 0.02), and lactate dehydrogenase (LDH, p = 0.01) activities in the CNT than in the CON group. Estradiol and progesterone did not differ between CNT and CON cows. Chestnut tannin supplementation improves antioxidant protection, prevents oxidation-reduction processes, reduces the degree of liver cell membrane damage, and protects thyroid tissue from damage, allowing higher T3 and T4 synthesis. Considering the importance of the thyroid hormone status before parturition, mechanisms of thyroid hormone regulation in CNT-supplemented dairy cows require more detailed investigations.",
publisher = "MDPI",
journal = "Metabolites",
title = "Dietary Supplementation of Chestnut Tannins in Prepartum Dairy Cows Improves Antioxidant Defense Mechanisms Interacting with Thyroid Status",
volume = "13",
number = "3",
pages = "334",
doi = "10.3390/metabo13030334"
}

Prodanović, R., Nedić, S., Vujanac, I., Bojkovski, J., Nedić, S., Jovanović, L., Kirovski, D.,& Borozan, S.. (2023). Dietary Supplementation of Chestnut Tannins in Prepartum Dairy Cows Improves Antioxidant Defense Mechanisms Interacting with Thyroid Status. in Metabolites
MDPI., 13(3), 334.
https://doi.org/10.3390/metabo13030334

Prodanović R, Nedić S, Vujanac I, Bojkovski J, Nedić S, Jovanović L, Kirovski D, Borozan S. Dietary Supplementation of Chestnut Tannins in Prepartum Dairy Cows Improves Antioxidant Defense Mechanisms Interacting with Thyroid Status. in Metabolites. 2023;13(3):334.
doi:10.3390/metabo13030334 .

Prodanović, Radiša, Nedić, Sreten, Vujanac, Ivan, Bojkovski, Jovan, Nedić, Svetlana, Jovanović, Ljubomir, Kirovski, Danijela, Borozan, Sunčica, "Dietary Supplementation of Chestnut Tannins in Prepartum Dairy Cows Improves Antioxidant Defense Mechanisms Interacting with Thyroid Status" in Metabolites, 13, no. 3 (2023):334,
https://doi.org/10.3390/metabo13030334 . .

TY  - JOUR
AU  - Janković, Dušan
AU  - Norrman, Johanna
AU  - Aronsson, Mia
AU  - Vojvodić, Danilo
AU  - Mirilović, Milorad
AU  - Vejnović, Branislav
AU  - Stanišić, Ljubodrag
AU  - Nedić, Svetlana
AU  - Maletić, Milan
AU  - Đurić, Miloje
AU  - Magaš, Vladimir
AU  - Vakanjac, Slobodanka
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2517
AB  - The work was aimed to research into the vital signs, haematological and biochemical parameters, acute-phase protein concentrations (CRP, albumin), progesterone (PG) and anti-Müllerian hormone (AMH) concentrations in bitches with pyometra and their comparison with those in healthy bitches. In addition, the goal of this work was to assess if some of the tested parameters may be used as a biomarker in the diagnostics of pyometra and the monitoring of the postoperative recovery. Forty 3-6-year-old bitches of various breeds were included in the research and allotted to two groups: the control (C, n = 20) and the diseased – bitches diagnosed with pyometra (P, n = 20). Blood samples for the analyses were taken immediately before surgery (0 h), and 12 h, 24 h, 48 h and 72 h after, excepting for AMH and PG concentrations, which were determined only at 0 h. Clinical examination detected significantly higher respiration rates in bitches with pyometra in comparison to healthy ones at all time points, higher heart rates before and 12 h after surgery, and higher body temperature before and 12 h, 48 h and 72 h post operation (p <.01). Significantly higher concentrations of CRP (p <.01) and lower concentrations of albumin were detected in bitches with pyometra in comparison to their healthy counterparts at all time points. Progesterone and AMH concentration analysis immediately before surgery detected significantly higher levels of the hormone in bitches with pyometra than in the control (p <.01). The inflammatory reaction of the endometrium resulted in a significant AMH concentration increase, whilst increased CRP concentrations and lower albumin concentrations throughout the research may be used as biomarkers of proinflammatory activities, that is of the acute-phase response.
PB  - John Wiley & Sons, Ltd
T2  - Reproduction in Domestic Animals
T1  - Analysis of the concentrations of some haematological parameters, C-reactive protein and anti-Müllerian hormone in bitches affected by pyometra
VL  - 57
IS  - 10
SP  - 1156
EP  - 1164
DO  - 10.1111/rda.14186
ER  - 

@article{
author = "Janković, Dušan and Norrman, Johanna and Aronsson, Mia and Vojvodić, Danilo and Mirilović, Milorad and Vejnović, Branislav and Stanišić, Ljubodrag and Nedić, Svetlana and Maletić, Milan and Đurić, Miloje and Magaš, Vladimir and Vakanjac, Slobodanka",
year = "2022",
abstract = "The work was aimed to research into the vital signs, haematological and biochemical parameters, acute-phase protein concentrations (CRP, albumin), progesterone (PG) and anti-Müllerian hormone (AMH) concentrations in bitches with pyometra and their comparison with those in healthy bitches. In addition, the goal of this work was to assess if some of the tested parameters may be used as a biomarker in the diagnostics of pyometra and the monitoring of the postoperative recovery. Forty 3-6-year-old bitches of various breeds were included in the research and allotted to two groups: the control (C, n = 20) and the diseased – bitches diagnosed with pyometra (P, n = 20). Blood samples for the analyses were taken immediately before surgery (0 h), and 12 h, 24 h, 48 h and 72 h after, excepting for AMH and PG concentrations, which were determined only at 0 h. Clinical examination detected significantly higher respiration rates in bitches with pyometra in comparison to healthy ones at all time points, higher heart rates before and 12 h after surgery, and higher body temperature before and 12 h, 48 h and 72 h post operation (p <.01). Significantly higher concentrations of CRP (p <.01) and lower concentrations of albumin were detected in bitches with pyometra in comparison to their healthy counterparts at all time points. Progesterone and AMH concentration analysis immediately before surgery detected significantly higher levels of the hormone in bitches with pyometra than in the control (p <.01). The inflammatory reaction of the endometrium resulted in a significant AMH concentration increase, whilst increased CRP concentrations and lower albumin concentrations throughout the research may be used as biomarkers of proinflammatory activities, that is of the acute-phase response.",
publisher = "John Wiley & Sons, Ltd",
journal = "Reproduction in Domestic Animals",
title = "Analysis of the concentrations of some haematological parameters, C-reactive protein and anti-Müllerian hormone in bitches affected by pyometra",
volume = "57",
number = "10",
pages = "1156-1164",
doi = "10.1111/rda.14186"
}

Janković, D., Norrman, J., Aronsson, M., Vojvodić, D., Mirilović, M., Vejnović, B., Stanišić, L., Nedić, S., Maletić, M., Đurić, M., Magaš, V.,& Vakanjac, S.. (2022). Analysis of the concentrations of some haematological parameters, C-reactive protein and anti-Müllerian hormone in bitches affected by pyometra. in Reproduction in Domestic Animals
John Wiley & Sons, Ltd., 57(10), 1156-1164.
https://doi.org/10.1111/rda.14186

Janković D, Norrman J, Aronsson M, Vojvodić D, Mirilović M, Vejnović B, Stanišić L, Nedić S, Maletić M, Đurić M, Magaš V, Vakanjac S. Analysis of the concentrations of some haematological parameters, C-reactive protein and anti-Müllerian hormone in bitches affected by pyometra. in Reproduction in Domestic Animals. 2022;57(10):1156-1164.
doi:10.1111/rda.14186 .

Janković, Dušan, Norrman, Johanna, Aronsson, Mia, Vojvodić, Danilo, Mirilović, Milorad, Vejnović, Branislav, Stanišić, Ljubodrag, Nedić, Svetlana, Maletić, Milan, Đurić, Miloje, Magaš, Vladimir, Vakanjac, Slobodanka, "Analysis of the concentrations of some haematological parameters, C-reactive protein and anti-Müllerian hormone in bitches affected by pyometra" in Reproduction in Domestic Animals, 57, no. 10 (2022):1156-1164,
https://doi.org/10.1111/rda.14186 . .

TY  - CONF
AU  - Đurić, Miloje
AU  - Nedić, Svetlana
AU  - Vakanjac, Slobodanka
AU  - Nestorović, Ivan
AU  - Pavlović, Miloš
AU  - Maletić, Milan
AU  - Stanišić, Ljubodrag
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3189
AB  - Libido, sposobnost parenja i kolekcija ejakulata kod pastuva može zavisiti od
mnogo faktora. Ovi faktori mogu biti nasledni, iz okruženja ili naučeni šabloni ponašanja
na koje veoma utiče ophođenje i nega pastuva. Kod veštačkog osemenjavanja
i programa konzerviranja semena efikasna kolekcija visoko kvalitetnog
ejakulata je od izuzetne važnosti. Kolekcija ejakulata je deo procene zdravstvenog
i priplodnog statusa kod pastuva pre ili posle kupovine/prodaje grla. Postupci
pri kolekciji i obradi ejakulata mogu biti uzrok lošeg kvaliteta semena. Prilikom
kolekcije ejakulata pastuva treba obratiti pažnju na prostor za kolekciju i metode
kolekcije od kojih je korišćenje veštačke vagine najčešći i najuspešniji metod kolekcije.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - 13. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 6 - 9. oktobar 2022
T1  - Kolekcija ejakulata pastuva – pristupi i kritične tačke
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3189
ER  - 

@conference{
author = "Đurić, Miloje and Nedić, Svetlana and Vakanjac, Slobodanka and Nestorović, Ivan and Pavlović, Miloš and Maletić, Milan and Stanišić, Ljubodrag",
year = "2022",
abstract = "Libido, sposobnost parenja i kolekcija ejakulata kod pastuva može zavisiti od
mnogo faktora. Ovi faktori mogu biti nasledni, iz okruženja ili naučeni šabloni ponašanja
na koje veoma utiče ophođenje i nega pastuva. Kod veštačkog osemenjavanja
i programa konzerviranja semena efikasna kolekcija visoko kvalitetnog
ejakulata je od izuzetne važnosti. Kolekcija ejakulata je deo procene zdravstvenog
i priplodnog statusa kod pastuva pre ili posle kupovine/prodaje grla. Postupci
pri kolekciji i obradi ejakulata mogu biti uzrok lošeg kvaliteta semena. Prilikom
kolekcije ejakulata pastuva treba obratiti pažnju na prostor za kolekciju i metode
kolekcije od kojih je korišćenje veštačke vagine najčešći i najuspešniji metod kolekcije.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "13. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 6 - 9. oktobar 2022",
title = "Kolekcija ejakulata pastuva – pristupi i kritične tačke",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3189"
}

Đurić, M., Nedić, S., Vakanjac, S., Nestorović, I., Pavlović, M., Maletić, M.,& Stanišić, L.. (2022). Kolekcija ejakulata pastuva – pristupi i kritične tačke. in 13. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 6 - 9. oktobar 2022
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila..
https://hdl.handle.net/21.15107/rcub_veterinar_3189

Đurić M, Nedić S, Vakanjac S, Nestorović I, Pavlović M, Maletić M, Stanišić L. Kolekcija ejakulata pastuva – pristupi i kritične tačke. in 13. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 6 - 9. oktobar 2022. 2022;.
https://hdl.handle.net/21.15107/rcub_veterinar_3189 .

Đurić, Miloje, Nedić, Svetlana, Vakanjac, Slobodanka, Nestorović, Ivan, Pavlović, Miloš, Maletić, Milan, Stanišić, Ljubodrag, "Kolekcija ejakulata pastuva – pristupi i kritične tačke" in 13. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 6 - 9. oktobar 2022 (2022),
https://hdl.handle.net/21.15107/rcub_veterinar_3189 .

TY  - JOUR
AU  - Jakovljević, Goran
AU  - Lazarević, Miodrag
AU  - Mirilović, Milorad
AU  - Milovanović, Aleksandar
AU  - Apić, Jelena
AU  - Šefer, Dragan
AU  - Nedić, Svetlana
AU  - Vakanjac, Slobodanka
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2267
AB  - This study aimed to assess the influence of different concentrations of zinc (Zn), lead (Pb), mercury (Hg) and cadmium (Cd) found in the feed and water provided to bulls on the concentrations of these metals in cryopreserved bull semen, and to determine their influence onto semen quality parameters. Correlations between heavy metal concentrations in the semen and the quality parameters of semen as estimated by computer-assisted sperm analysis (CASA) and flow cytometry (FC) methods were determined. A total of 40 cryopreserved semen samples originating from bulls housed in 4 different centers for artificial insemination (A, B, C and D) were examined, making a total of 160 samples. The concentrations of metals and semen quality parameters were determined in cryopreserved semen of 10 bulls from each center, namely 4 samples from each bull. Concentrations of Zn, Pb, Hg and Cd in hay and concentrated feed were within the allowed limits as proposed by the National Research Council (NRC, 2000). A strong negative correlation was detected between curvilinear velocity (VCL) and Zn concentrations in the semen (P < 0.01; r = -0.772) in group D, and a positive correlation of VCL with Pb concentrations (P < 0.05 and r = 0.718) in group B. Mercury concentrations in cryopreserved semen correlated negatively to the percent of live sperm cells with intact acrosomes (V/IA: P < 0.05; r = -0.640) and positively with the percent of dead sperm cells with damaged acrosomes (D/DA: P < 0.01; r = 0.766) in group D. This finding confirms the hypothesis that Hg, even at low concentrations, may cause acrosome damage.
AB  - Cilj rada bio je da se ispita uticaj različitih koncentracija cinka (Zn), olova (Pb), žive
(Hg) i kadmijuma (Cd) iz hrane i vode na koncentracije ovih metala u duboko zamrznutom semenu nakon otapanja i da se ispita njihov uticaj na parametre kvaliteta
semena. Korelacije između koncentracije teških metala u semenu i parametra kvaliteta semena su određivane nakon ispitivanja semena kompjuterski asistiranom analizom
semena (eng. computer-assisted sperm analysis - CASA) i protočnom citometrijom
(eng. flow cytometry - FC).
Iz 4 različita centra za veštačko osemenjavanje (A, B, C i D) ispitivano je po 40 uzoraka duboko zamrznutog semena bikova, što čini ukupno 160 uzoraka. Koncentracije
metala i parametri kvaliteta semena su određivani u duboko zamrznutom semenu od
10 bikova iz svakog centra, odnosno 4 uzorka od svakog bika. Koncentracije Zn, Pb,
Hg i Cd u senu i koncentrovanim hranivima bile su ispod propisanih dozvoljenih
vrednosti Nacionalnog Istraživačkog Veća (eng. National Research Council - NRC,
2000). Jaka negativna korelacija uočena je između krivolinijske brzine spermatozoida
(eng. curvilinear velocity - VCL) i koncentracije Zn u semenu (P < 0.01; r = -0.772) u
grupi D, i pozitivna korelacija između VCL i koncentracije Pb (P < 0.05 and r = 0.718)
u grupi B. Koncentracija žive u duboko zamrznutom semenu negativno je korelirala
sa procentom živih spermatozoida sa neoštećenim akrozomom (eng. live sperm cells
with intact acrosomes - V/IA: P < 0.05; r = -0.640) i pozitivno sa procentom mrtvih
spermatozoida sa oštećenim akrozomom (eng. dead sperm cells with damaged acrosomes - D/DA: P < 0.01; r = 0.766) u grupi D. Ovi rezultati potvrđuju hipotezu da Hg,
čak i u niskim koncenracijama može da prouzrokuje oštećenje akrozoma.
PB  - Fakultet veterinarske medicine
T2  - Acta veterinaria-Beograd
T1  - The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen
T1  - Uticaj cinka i teških metala iz hrane i vode na kvalitet duboko zamrznutog semena bikova
VL  - 71
IS  - 3
SP  - 326
EP  - 343
DO  - 10.2478/acve-2021-0028
ER  - 

@article{
author = "Jakovljević, Goran and Lazarević, Miodrag and Mirilović, Milorad and Milovanović, Aleksandar and Apić, Jelena and Šefer, Dragan and Nedić, Svetlana and Vakanjac, Slobodanka",
year = "2021",
abstract = "This study aimed to assess the influence of different concentrations of zinc (Zn), lead (Pb), mercury (Hg) and cadmium (Cd) found in the feed and water provided to bulls on the concentrations of these metals in cryopreserved bull semen, and to determine their influence onto semen quality parameters. Correlations between heavy metal concentrations in the semen and the quality parameters of semen as estimated by computer-assisted sperm analysis (CASA) and flow cytometry (FC) methods were determined. A total of 40 cryopreserved semen samples originating from bulls housed in 4 different centers for artificial insemination (A, B, C and D) were examined, making a total of 160 samples. The concentrations of metals and semen quality parameters were determined in cryopreserved semen of 10 bulls from each center, namely 4 samples from each bull. Concentrations of Zn, Pb, Hg and Cd in hay and concentrated feed were within the allowed limits as proposed by the National Research Council (NRC, 2000). A strong negative correlation was detected between curvilinear velocity (VCL) and Zn concentrations in the semen (P < 0.01; r = -0.772) in group D, and a positive correlation of VCL with Pb concentrations (P < 0.05 and r = 0.718) in group B. Mercury concentrations in cryopreserved semen correlated negatively to the percent of live sperm cells with intact acrosomes (V/IA: P < 0.05; r = -0.640) and positively with the percent of dead sperm cells with damaged acrosomes (D/DA: P < 0.01; r = 0.766) in group D. This finding confirms the hypothesis that Hg, even at low concentrations, may cause acrosome damage., Cilj rada bio je da se ispita uticaj različitih koncentracija cinka (Zn), olova (Pb), žive
(Hg) i kadmijuma (Cd) iz hrane i vode na koncentracije ovih metala u duboko zamrznutom semenu nakon otapanja i da se ispita njihov uticaj na parametre kvaliteta
semena. Korelacije između koncentracije teških metala u semenu i parametra kvaliteta semena su određivane nakon ispitivanja semena kompjuterski asistiranom analizom
semena (eng. computer-assisted sperm analysis - CASA) i protočnom citometrijom
(eng. flow cytometry - FC).
Iz 4 različita centra za veštačko osemenjavanje (A, B, C i D) ispitivano je po 40 uzoraka duboko zamrznutog semena bikova, što čini ukupno 160 uzoraka. Koncentracije
metala i parametri kvaliteta semena su određivani u duboko zamrznutom semenu od
10 bikova iz svakog centra, odnosno 4 uzorka od svakog bika. Koncentracije Zn, Pb,
Hg i Cd u senu i koncentrovanim hranivima bile su ispod propisanih dozvoljenih
vrednosti Nacionalnog Istraživačkog Veća (eng. National Research Council - NRC,
2000). Jaka negativna korelacija uočena je između krivolinijske brzine spermatozoida
(eng. curvilinear velocity - VCL) i koncentracije Zn u semenu (P < 0.01; r = -0.772) u
grupi D, i pozitivna korelacija između VCL i koncentracije Pb (P < 0.05 and r = 0.718)
u grupi B. Koncentracija žive u duboko zamrznutom semenu negativno je korelirala
sa procentom živih spermatozoida sa neoštećenim akrozomom (eng. live sperm cells
with intact acrosomes - V/IA: P < 0.05; r = -0.640) i pozitivno sa procentom mrtvih
spermatozoida sa oštećenim akrozomom (eng. dead sperm cells with damaged acrosomes - D/DA: P < 0.01; r = 0.766) u grupi D. Ovi rezultati potvrđuju hipotezu da Hg,
čak i u niskim koncenracijama može da prouzrokuje oštećenje akrozoma.",
publisher = "Fakultet veterinarske medicine",
journal = "Acta veterinaria-Beograd",
title = "The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen, Uticaj cinka i teških metala iz hrane i vode na kvalitet duboko zamrznutog semena bikova",
volume = "71",
number = "3",
pages = "326-343",
doi = "10.2478/acve-2021-0028"
}

Jakovljević, G., Lazarević, M., Mirilović, M., Milovanović, A., Apić, J., Šefer, D., Nedić, S.,& Vakanjac, S.. (2021). The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen. in Acta veterinaria-Beograd
Fakultet veterinarske medicine., 71(3), 326-343.
https://doi.org/10.2478/acve-2021-0028

Jakovljević G, Lazarević M, Mirilović M, Milovanović A, Apić J, Šefer D, Nedić S, Vakanjac S. The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen. in Acta veterinaria-Beograd. 2021;71(3):326-343.
doi:10.2478/acve-2021-0028 .

Jakovljević, Goran, Lazarević, Miodrag, Mirilović, Milorad, Milovanović, Aleksandar, Apić, Jelena, Šefer, Dragan, Nedić, Svetlana, Vakanjac, Slobodanka, "The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen" in Acta veterinaria-Beograd, 71, no. 3 (2021):326-343,
https://doi.org/10.2478/acve-2021-0028 . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Nedić, Sreten
AU  - Simeunović, Predrag
AU  - Borozan, Sunčica
AU  - Nedić, Svetlana
AU  - Bojkovski, Jovan
AU  - Kirovski, Danijela
AU  - Vujanac, Ivan
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2238
AB  - This study investigated the effects of dietary supplementation with chestnut tannins (CNT) on metabolic and antioxidant status of prepartum cows along with their colostrum quality. Pregnant multiparous Holstein cows were paired according to parity and body condition score, and assigned either to a diet supplemented with 20 g/d of commercially available product containing chestnut tannins (CNT, n=20) or to an unsupplemented control diet (CON, n=20) for the last 25±2 d of pregnancy. Serum metabolite, insulin and antioxidant capacity indices were measured in blood samples taken at d 25 and d 5 before expected parturition. Chemical composition and IgG concentration were determined in colostrum samples collected from the first milking postpartum. The addition of CNT led to lower BUN (P=0.02) and consequently higher serum glucose (P=0.02) and insulin (P<0.01) concentrations which were associated with lower circulating NEFA (P<0.01) and BHBA (P<0.01) in CNT group than those of CON. The serum paraoxonase 1 (PON 1) activity and total antioxidant capacity (T-AOC) were higher at-5 d in CNT than in CON (P<0.01, P=0.03; respectively). Close-up CNT improved lactose percentage and IgG concentration (P=0.03, P=0.04; respectively) and tended to improve percentage of protein and SNF (Solid Not Fat) in primary colostrum (P=0.06, respectively), without affecting colostrum fat and total solid (P=0.98, P=0.43; respectively). Supplementation of CNT in the diet during close-up period did not have adverse effects on metabolic profiles prepartum. Instead, this feeding regimen was more beneficial to antioxidant capacity and colostrum quality than feeding the control diet.
PB  - Sciendo
T2  - Annals of Animal Science
T1  - Effects of chestnut tannins supplementation of prepartum moderate yielding dairy cows on metabolic health, antioxidant and colostrum indices
VL  - 21
IS  - 2
SP  - 609
EP  - 621
DO  - 10.2478/aoas-2020-0077
ER  - 

@article{
author = "Prodanović, Radiša and Nedić, Sreten and Simeunović, Predrag and Borozan, Sunčica and Nedić, Svetlana and Bojkovski, Jovan and Kirovski, Danijela and Vujanac, Ivan",
year = "2021",
abstract = "This study investigated the effects of dietary supplementation with chestnut tannins (CNT) on metabolic and antioxidant status of prepartum cows along with their colostrum quality. Pregnant multiparous Holstein cows were paired according to parity and body condition score, and assigned either to a diet supplemented with 20 g/d of commercially available product containing chestnut tannins (CNT, n=20) or to an unsupplemented control diet (CON, n=20) for the last 25±2 d of pregnancy. Serum metabolite, insulin and antioxidant capacity indices were measured in blood samples taken at d 25 and d 5 before expected parturition. Chemical composition and IgG concentration were determined in colostrum samples collected from the first milking postpartum. The addition of CNT led to lower BUN (P=0.02) and consequently higher serum glucose (P=0.02) and insulin (P<0.01) concentrations which were associated with lower circulating NEFA (P<0.01) and BHBA (P<0.01) in CNT group than those of CON. The serum paraoxonase 1 (PON 1) activity and total antioxidant capacity (T-AOC) were higher at-5 d in CNT than in CON (P<0.01, P=0.03; respectively). Close-up CNT improved lactose percentage and IgG concentration (P=0.03, P=0.04; respectively) and tended to improve percentage of protein and SNF (Solid Not Fat) in primary colostrum (P=0.06, respectively), without affecting colostrum fat and total solid (P=0.98, P=0.43; respectively). Supplementation of CNT in the diet during close-up period did not have adverse effects on metabolic profiles prepartum. Instead, this feeding regimen was more beneficial to antioxidant capacity and colostrum quality than feeding the control diet.",
publisher = "Sciendo",
journal = "Annals of Animal Science",
title = "Effects of chestnut tannins supplementation of prepartum moderate yielding dairy cows on metabolic health, antioxidant and colostrum indices",
volume = "21",
number = "2",
pages = "609-621",
doi = "10.2478/aoas-2020-0077"
}

Prodanović, R., Nedić, S., Simeunović, P., Borozan, S., Nedić, S., Bojkovski, J., Kirovski, D.,& Vujanac, I.. (2021). Effects of chestnut tannins supplementation of prepartum moderate yielding dairy cows on metabolic health, antioxidant and colostrum indices. in Annals of Animal Science
Sciendo., 21(2), 609-621.
https://doi.org/10.2478/aoas-2020-0077

Prodanović R, Nedić S, Simeunović P, Borozan S, Nedić S, Bojkovski J, Kirovski D, Vujanac I. Effects of chestnut tannins supplementation of prepartum moderate yielding dairy cows on metabolic health, antioxidant and colostrum indices. in Annals of Animal Science. 2021;21(2):609-621.
doi:10.2478/aoas-2020-0077 .

Prodanović, Radiša, Nedić, Sreten, Simeunović, Predrag, Borozan, Sunčica, Nedić, Svetlana, Bojkovski, Jovan, Kirovski, Danijela, Vujanac, Ivan, "Effects of chestnut tannins supplementation of prepartum moderate yielding dairy cows on metabolic health, antioxidant and colostrum indices" in Annals of Animal Science, 21, no. 2 (2021):609-621,
https://doi.org/10.2478/aoas-2020-0077 . .

TY  - CONF
AU  - Bojkovski, Jovan
AU  - Prodanov-Radulović, Jasna
AU  - Vakanjac, Slobodanka
AU  - Becskei, Zsolt
AU  - Zdravković, Nemanja
AU  - Stanišić, Ljubodrag
AU  - Đurić, Miloje
AU  - Nedić, Svetlana
AU  - Dobrosavljević, Ivan
AU  - Maletić, Jelena
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3535
AB  - Biosigurnost, doborbit, dobra proizvođačka praksa i analiza rizika u kritičnim
kontrolim tačkama su veoma značajni elementi u intenzivnoj proizvodnji svinja.
Planska primena biosigurnosnih mera je presudna u zaštiti zdravlja svinja i uspehu same proizvodnje. Cilj ovog preglednog rada je da prikaže procenu biosigurnosnih mera na komercijalnim farmama svinja i da ukaže na moguće propuste na
farmama. U radu je diskutovano o mogućim rešenjima biosigurnosti na farmi i o
rutinskim postupcima u zaštiti od moguće biološke kontaminacije na komercijalnim farmama kao i odnosu proizvođača prema zaštiti zdravlja svinja.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020
T1  - Najčešći propusti u obezbeđivanju biosigurnosti na farmi svinja
SP  - 185
EP  - 195
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3535
ER  - 

@conference{
author = "Bojkovski, Jovan and Prodanov-Radulović, Jasna and Vakanjac, Slobodanka and Becskei, Zsolt and Zdravković, Nemanja and Stanišić, Ljubodrag and Đurić, Miloje and Nedić, Svetlana and Dobrosavljević, Ivan and Maletić, Jelena",
year = "2020",
abstract = "Biosigurnost, doborbit, dobra proizvođačka praksa i analiza rizika u kritičnim
kontrolim tačkama su veoma značajni elementi u intenzivnoj proizvodnji svinja.
Planska primena biosigurnosnih mera je presudna u zaštiti zdravlja svinja i uspehu same proizvodnje. Cilj ovog preglednog rada je da prikaže procenu biosigurnosnih mera na komercijalnim farmama svinja i da ukaže na moguće propuste na
farmama. U radu je diskutovano o mogućim rešenjima biosigurnosti na farmi i o
rutinskim postupcima u zaštiti od moguće biološke kontaminacije na komercijalnim farmama kao i odnosu proizvođača prema zaštiti zdravlja svinja.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020",
title = "Najčešći propusti u obezbeđivanju biosigurnosti na farmi svinja",
pages = "185-195",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3535"
}

Bojkovski, J., Prodanov-Radulović, J., Vakanjac, S., Becskei, Z., Zdravković, N., Stanišić, L., Đurić, M., Nedić, S., Dobrosavljević, I.,& Maletić, J.. (2020). Najčešći propusti u obezbeđivanju biosigurnosti na farmi svinja. in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 185-195.
https://hdl.handle.net/21.15107/rcub_veterinar_3535

Bojkovski J, Prodanov-Radulović J, Vakanjac S, Becskei Z, Zdravković N, Stanišić L, Đurić M, Nedić S, Dobrosavljević I, Maletić J. Najčešći propusti u obezbeđivanju biosigurnosti na farmi svinja. in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020. 2020;:185-195.
https://hdl.handle.net/21.15107/rcub_veterinar_3535 .

Bojkovski, Jovan, Prodanov-Radulović, Jasna, Vakanjac, Slobodanka, Becskei, Zsolt, Zdravković, Nemanja, Stanišić, Ljubodrag, Đurić, Miloje, Nedić, Svetlana, Dobrosavljević, Ivan, Maletić, Jelena, "Najčešći propusti u obezbeđivanju biosigurnosti na farmi svinja" in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020 (2020):185-195,
https://hdl.handle.net/21.15107/rcub_veterinar_3535 .

TY  - CONF
AU  - Aleksić, Jelena
AU  - Kirovski, Danijela
AU  - Stojanac, Nenad
AU  - Nedić, Svetlana
AU  - Vakanjac, Slobodanka
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3809
AB  - U radu je dat pregled zakonodavstva Republike Srbije iz oblasti animalne reprodukcije, kao i zakonodavstva Evropske Unije iz oblasti reprodukcije, embriotransfera, uslova koje treba da ispune timovi za manipulaciju embrionima i uslova
za izvoz embriona iz trećih zemalja u zemlje članice EU. Ukazano na neophodnost donošenja normativnih akta kojima bi se definisali uslovi za postojanje i rad
laboratorija u pogledu objekata (prostorija), opreme, sredstava za rad i stručnog
kadra u kojima bi se obavljala manipulacija embrionima i jajnim ćelijama. Na ovaj
način će znanja i veštine specijalista animalne biotehnologije u reprodukciji, stečena tokom studija na akreditovanim specijalističkim studijama na Katedri za porodiljstvo, reprodukciju i veštačko osemenjavanje Fakulteta veterinarske medicine
Univerziteta u Beogradu biti na pravi način iskorišćena u razvoju animalne biotehnologije u veterinarskoj medicini u Srbiji.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020
T1  - Savremene tehnike u animalnoj reprodukciji u svetlu važećih propisa
SP  - 3
EP  - 12
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3809
ER  - 

@conference{
author = "Aleksić, Jelena and Kirovski, Danijela and Stojanac, Nenad and Nedić, Svetlana and Vakanjac, Slobodanka",
year = "2020",
abstract = "U radu je dat pregled zakonodavstva Republike Srbije iz oblasti animalne reprodukcije, kao i zakonodavstva Evropske Unije iz oblasti reprodukcije, embriotransfera, uslova koje treba da ispune timovi za manipulaciju embrionima i uslova
za izvoz embriona iz trećih zemalja u zemlje članice EU. Ukazano na neophodnost donošenja normativnih akta kojima bi se definisali uslovi za postojanje i rad
laboratorija u pogledu objekata (prostorija), opreme, sredstava za rad i stručnog
kadra u kojima bi se obavljala manipulacija embrionima i jajnim ćelijama. Na ovaj
način će znanja i veštine specijalista animalne biotehnologije u reprodukciji, stečena tokom studija na akreditovanim specijalističkim studijama na Katedri za porodiljstvo, reprodukciju i veštačko osemenjavanje Fakulteta veterinarske medicine
Univerziteta u Beogradu biti na pravi način iskorišćena u razvoju animalne biotehnologije u veterinarskoj medicini u Srbiji.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020",
title = "Savremene tehnike u animalnoj reprodukciji u svetlu važećih propisa",
pages = "3-12",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3809"
}

Aleksić, J., Kirovski, D., Stojanac, N., Nedić, S.,& Vakanjac, S.. (2020). Savremene tehnike u animalnoj reprodukciji u svetlu važećih propisa. in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 3-12.
https://hdl.handle.net/21.15107/rcub_veterinar_3809

Aleksić J, Kirovski D, Stojanac N, Nedić S, Vakanjac S. Savremene tehnike u animalnoj reprodukciji u svetlu važećih propisa. in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020. 2020;:3-12.
https://hdl.handle.net/21.15107/rcub_veterinar_3809 .

Aleksić, Jelena, Kirovski, Danijela, Stojanac, Nenad, Nedić, Svetlana, Vakanjac, Slobodanka, "Savremene tehnike u animalnoj reprodukciji u svetlu važećih propisa" in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020 (2020):3-12,
https://hdl.handle.net/21.15107/rcub_veterinar_3809 .

TY  - JOUR
AU  - Nedić, Svetlana
AU  - Vakanjac, Slobodanka
AU  - Samardzija, Marko
AU  - Borozan, Sunčica
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1782
AB  - The aim of this study was to determine serum lipid values and parameters of oxidative stress in blood and milk of cows with subclinical mastitis (SCM) caused by Staphylococcus aureus and to establish association between these parameters. The study was performed on total of 104 cows assigned into control group of healthy animals (n = 12) and two groups of cows with SCM, either SCM1 group (n = 37) with < 1000 CFU/mL of S. aureus or SCM2 group (n = 55) with >= 1000 CFU/mL of causative agent in milk. Significantly lower serum concentrations of high density lipoprotein-cholesterol (HDL-C) in SCM2 group and higher low density lipoprotein-cholesterol (LDL-C) in both SCM groups were recorded. Significantly lower paraoxonase-1 (PON1) activity and higher lipid hydroperoxides (LOOH) concentration in blood and milk were recorded in both groups of SCM cows vs control. In blood serum of cows from SCM2 group significantly higher concentrations of hydrogen peroxide (H2O2) and malondialdehyde (MDA), but significantly lower total antioxidative capacity (TAC) were obtained. In milk serum of SCM cows were detected significantly higher concentrations of H2O2 and MDA, but significantly lower TAC vs control. There was a significant positive correlation between PON1 in serum with HDL-C, but negative correlation with LDL-C and LOOH. In milk, PON1 negatively correlated with LOOH and somatic cell counts (SCC). Strong positive correlation was obtained between PON1 in blood and milk. Oxidative stress and inflammatory reaction induced by SCM significantly lowered PON1 activity in blood and milk of affected cows.
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus
VL  - 125
SP  - 323
EP  - 332
DO  - 10.1016/j.rvsc.2019.07.016
ER  - 

@article{
author = "Nedić, Svetlana and Vakanjac, Slobodanka and Samardzija, Marko and Borozan, Sunčica",
year = "2019",
abstract = "The aim of this study was to determine serum lipid values and parameters of oxidative stress in blood and milk of cows with subclinical mastitis (SCM) caused by Staphylococcus aureus and to establish association between these parameters. The study was performed on total of 104 cows assigned into control group of healthy animals (n = 12) and two groups of cows with SCM, either SCM1 group (n = 37) with < 1000 CFU/mL of S. aureus or SCM2 group (n = 55) with >= 1000 CFU/mL of causative agent in milk. Significantly lower serum concentrations of high density lipoprotein-cholesterol (HDL-C) in SCM2 group and higher low density lipoprotein-cholesterol (LDL-C) in both SCM groups were recorded. Significantly lower paraoxonase-1 (PON1) activity and higher lipid hydroperoxides (LOOH) concentration in blood and milk were recorded in both groups of SCM cows vs control. In blood serum of cows from SCM2 group significantly higher concentrations of hydrogen peroxide (H2O2) and malondialdehyde (MDA), but significantly lower total antioxidative capacity (TAC) were obtained. In milk serum of SCM cows were detected significantly higher concentrations of H2O2 and MDA, but significantly lower TAC vs control. There was a significant positive correlation between PON1 in serum with HDL-C, but negative correlation with LDL-C and LOOH. In milk, PON1 negatively correlated with LOOH and somatic cell counts (SCC). Strong positive correlation was obtained between PON1 in blood and milk. Oxidative stress and inflammatory reaction induced by SCM significantly lowered PON1 activity in blood and milk of affected cows.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus",
volume = "125",
pages = "323-332",
doi = "10.1016/j.rvsc.2019.07.016"
}

Nedić, S., Vakanjac, S., Samardzija, M.,& Borozan, S.. (2019). Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 125, 323-332.
https://doi.org/10.1016/j.rvsc.2019.07.016

Nedić S, Vakanjac S, Samardzija M, Borozan S. Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus. in Research in Veterinary Science. 2019;125:323-332.
doi:10.1016/j.rvsc.2019.07.016 .

Nedić, Svetlana, Vakanjac, Slobodanka, Samardzija, Marko, Borozan, Sunčica, "Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus" in Research in Veterinary Science, 125 (2019):323-332,
https://doi.org/10.1016/j.rvsc.2019.07.016 . .

TY  - CONF
AU  - Becskei, Zsolt
AU  - Savić, Mila
AU  - Gáspárdy, András
AU  - Nedić, Svetlana
AU  - Bojkovski, Jovan
AU  - Rašeta, Mladen
AU  - Horvat, Jožef
AU  - Kovács, Endre
AU  - Milovanović, Aleksandar
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3526
PB  - University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca
C3  - 18th International Conference Life sciences for sustainable development, Cluj Napoca Romania,26th - 28th September, 2019
T1  - Breeding rams of endangered Lipe Zackel sheep - assessment of some reproductive traits
SP  - 137
EP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3526
ER  - 

@conference{
author = "Becskei, Zsolt and Savić, Mila and Gáspárdy, András and Nedić, Svetlana and Bojkovski, Jovan and Rašeta, Mladen and Horvat, Jožef and Kovács, Endre and Milovanović, Aleksandar",
year = "2019",
publisher = "University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca",
journal = "18th International Conference Life sciences for sustainable development, Cluj Napoca Romania,26th - 28th September, 2019",
title = "Breeding rams of endangered Lipe Zackel sheep - assessment of some reproductive traits",
pages = "137-137",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3526"
}

Becskei, Z., Savić, M., Gáspárdy, A., Nedić, S., Bojkovski, J., Rašeta, M., Horvat, J., Kovács, E.,& Milovanović, A.. (2019). Breeding rams of endangered Lipe Zackel sheep - assessment of some reproductive traits. in 18th International Conference Life sciences for sustainable development, Cluj Napoca Romania,26th - 28th September, 2019
University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca., 137-137.
https://hdl.handle.net/21.15107/rcub_veterinar_3526

Becskei Z, Savić M, Gáspárdy A, Nedić S, Bojkovski J, Rašeta M, Horvat J, Kovács E, Milovanović A. Breeding rams of endangered Lipe Zackel sheep - assessment of some reproductive traits. in 18th International Conference Life sciences for sustainable development, Cluj Napoca Romania,26th - 28th September, 2019. 2019;:137-137.
https://hdl.handle.net/21.15107/rcub_veterinar_3526 .

Becskei, Zsolt, Savić, Mila, Gáspárdy, András, Nedić, Svetlana, Bojkovski, Jovan, Rašeta, Mladen, Horvat, Jožef, Kovács, Endre, Milovanović, Aleksandar, "Breeding rams of endangered Lipe Zackel sheep - assessment of some reproductive traits" in 18th International Conference Life sciences for sustainable development, Cluj Napoca Romania,26th - 28th September, 2019 (2019):137-137,
https://hdl.handle.net/21.15107/rcub_veterinar_3526 .

TY  - JOUR
AU  - Marinković, Darko
AU  - Aničić, Milan
AU  - Vakanjac, Slobodanka
AU  - Nedić, Svetlana
AU  - Magaš, Vladimir
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1590
AB  - The estrus cycle of bitches is divided into four phases: proestrus, estrus, diestrus and anestrus, during which different morphological changes, and also cyclic changes of estrogen and progesterone receptors are present. Several pathological changes can be differentiated on the endometrium, but one of these is the most important - cystic endometrial hyperplasia, which frequently develops into pyometra. The aim of the present study was to describe morphological characteristics, and expression of estrogen and progesterone receptors on the endometrium of mixed-breed bitches during the different phases of the estrus cycle, cystic endometrial hyperplasia and pyometra. The uterus and ovaries of 36 mixed breed bitches in different phases of the estrus cycle and also with cystic endometrial hyperplasia (CEH) and chronic purulent endometritis - pyometra were examined macroscopically, histopathologically, and immunohistochemically for estrogen receptors (ER) and progesterone receptors (PR). During proestrus uterine cells showed a weak reaction for both estrogen and progesterone receptors, but during estrus a large number of uterine cells showed a strong reaction on estrogen receptors and moderate reaction on progesterone receptors. On the contrary, during diestrus the scores for the estrogen receptors decreased, while the progesterone receptors level increased - uterine cells expressed strong reaction for progesterone receptors, and moderate reaction for estrogen receptors. Uterine cells in cystic endometrial hyperplasia expressed a strong reaction for estrogen receptors, and moderate reaction for progesterone receptors, but on the other hand the uterine cells in the uterus with pyometra expressed a moderate to strong reaction for progesterone receptors, and a weak reaction for estrogen receptors. In further investigations it would be interesting to perform quantitative analysis for both estrogen and progesterone receptors during different phases of the estrus cycle and also in the uterus with cystic endometrial hyperplasia and pyometra.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Morphological characteristics and expression of estrogen and progesterone receptors in the canine endometrium during the estrus cycle, cystic endometrial hyperplasia and pyometra
VL  - 68
IS  - 3
SP  - 239
EP  - 250
DO  - 10.2478/acve-2018-0021
ER  - 

@article{
author = "Marinković, Darko and Aničić, Milan and Vakanjac, Slobodanka and Nedić, Svetlana and Magaš, Vladimir",
year = "2018",
abstract = "The estrus cycle of bitches is divided into four phases: proestrus, estrus, diestrus and anestrus, during which different morphological changes, and also cyclic changes of estrogen and progesterone receptors are present. Several pathological changes can be differentiated on the endometrium, but one of these is the most important - cystic endometrial hyperplasia, which frequently develops into pyometra. The aim of the present study was to describe morphological characteristics, and expression of estrogen and progesterone receptors on the endometrium of mixed-breed bitches during the different phases of the estrus cycle, cystic endometrial hyperplasia and pyometra. The uterus and ovaries of 36 mixed breed bitches in different phases of the estrus cycle and also with cystic endometrial hyperplasia (CEH) and chronic purulent endometritis - pyometra were examined macroscopically, histopathologically, and immunohistochemically for estrogen receptors (ER) and progesterone receptors (PR). During proestrus uterine cells showed a weak reaction for both estrogen and progesterone receptors, but during estrus a large number of uterine cells showed a strong reaction on estrogen receptors and moderate reaction on progesterone receptors. On the contrary, during diestrus the scores for the estrogen receptors decreased, while the progesterone receptors level increased - uterine cells expressed strong reaction for progesterone receptors, and moderate reaction for estrogen receptors. Uterine cells in cystic endometrial hyperplasia expressed a strong reaction for estrogen receptors, and moderate reaction for progesterone receptors, but on the other hand the uterine cells in the uterus with pyometra expressed a moderate to strong reaction for progesterone receptors, and a weak reaction for estrogen receptors. In further investigations it would be interesting to perform quantitative analysis for both estrogen and progesterone receptors during different phases of the estrus cycle and also in the uterus with cystic endometrial hyperplasia and pyometra.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Morphological characteristics and expression of estrogen and progesterone receptors in the canine endometrium during the estrus cycle, cystic endometrial hyperplasia and pyometra",
volume = "68",
number = "3",
pages = "239-250",
doi = "10.2478/acve-2018-0021"
}

Marinković, D., Aničić, M., Vakanjac, S., Nedić, S.,& Magaš, V.. (2018). Morphological characteristics and expression of estrogen and progesterone receptors in the canine endometrium during the estrus cycle, cystic endometrial hyperplasia and pyometra. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(3), 239-250.
https://doi.org/10.2478/acve-2018-0021

Marinković D, Aničić M, Vakanjac S, Nedić S, Magaš V. Morphological characteristics and expression of estrogen and progesterone receptors in the canine endometrium during the estrus cycle, cystic endometrial hyperplasia and pyometra. in Acta Veterinaria-Beograd. 2018;68(3):239-250.
doi:10.2478/acve-2018-0021 .

Marinković, Darko, Aničić, Milan, Vakanjac, Slobodanka, Nedić, Svetlana, Magaš, Vladimir, "Morphological characteristics and expression of estrogen and progesterone receptors in the canine endometrium during the estrus cycle, cystic endometrial hyperplasia and pyometra" in Acta Veterinaria-Beograd, 68, no. 3 (2018):239-250,
https://doi.org/10.2478/acve-2018-0021 . .

TY  - JOUR
AU  - Đurić, Miloje
AU  - Jovanović, Ljubomir
AU  - Aničić, Milan
AU  - Nedić, Svetlana
AU  - Nedić, Sreten
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1445
AB  - An apathetic 14-year-old Hungarian Puli bitch was presented with a seven day history of vaginal discharge and reported ovariohysterectomy (OHE) conducted six years ago. Clinical examination showed elevated body temperature (39.5°C), enlarged abdomen, and vulvar swelling. Despite a reported OHE in the anamnesis, ultrasound examination of the abdomen demonstrated the presence of both ovaries and a large anechogenic zone resembling the uterus. Vaginal cytologic examination and hormonal analysis indicated that the bitch was in the oestrus phase of the cycle. After laparotomy, both intact ovaries and an enlarged uterus with marked adhesions and filled with liquid content were visualised. The cervix was situated in the caudal part of the abdominal cavity and connected to the uterus by only an adhesion stump. After extirpation, uterus and ovarian tissue samples were sent for histological examination. Histological findings of uterus and ovarian tissue samples indicated a diagnosis of mucometra. Obviously, OHE was not conducted professionally, and the procedure performed only prevented conception, but ovaries and uterus continued to be active and caused a serious health disorder after six years. When only a part of the ovaries or uterus is left after OHE, health complications can appear up to ten years later. Our case testifies that even if both ovaries and the entire uterus are left after OHE without communication with cervix and vagina, a bitch can live without noticeable health disorders up to six years. This underlines the importance of lymphatic drainage and resorption processes in the uterus as well as evacuation of uterus content through the vagina.
AB  - Četrnaest godina stara kuja rase mađarski puli je dovedena u apatičnom stanju i sa anamnezom iscedka iz vagine koji je prisutan 7 dana. Prema rečima vlasnika ovariohisterektomija je urađena pre 6 godina. Kliničkim nalazom je utvrđena povišena telesna temperatura (39,5˚C) uvećan abdomen i otok vulve. Uprkos podacima iz anamneze ultrazvučni pregled je ukazivao na prisustvo oba jajnika i velike anehogene zone koja je ukazivala na prisustvo materice. Citološki nalaz brisa vagine i hormonska analiza su ukazivali na to da je kuja bila u estralnoj fazi ciklusa. Nakon laparotomije potvrđeno je prisustvo oba jajnika i uvećane materice koja je bila ispunjena tečnim sadržajem i sa velikim brojem adhezija. Cerviks je bio lociran u kaudalnom delu abdomena i pričvršćen za uterus samo adhezionim patrljkom. Nakon ekstirpacije uterus i ovarijumi su poslati na histološku analizu, kojom je utvrđena dijagnoza mukometre. Očigledno da prethodno urađena ovariohisterektomija nije bila obavljena stručno i da je urađeni postupak sprečio koncepciju ali su ovarijumi i materica nastavili da budu aktivni što je prouzrokovalo ozbiljan zdravstveni problem 6 godina kasnije. Kada nakon ovariohisterektomije ostanu samo delovi tkiva ovarijuma i uterusa zdravstvene komplikacije se mogu javiti i do 10 godina nakon operacije. Prikazani slučaj svedoči o tome da je, čak i u slučaju kada su nakon operacije ostavljeni oba ovarijuma i uterus bez komunikacije sa cerviksom i vaginom, kuja 6 godina živela bez ozbiljnijih zdravstvenih problema. Ovo naglašava značaj limfne drenaže i procesa resorpcije sadržaja iz materice, pored pražnjenja kroz cerviks i vaginu.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Mucometra in a bitch as a consequence of a serious professional mistake
T1  - Mukometra kao posledica ozbiljne stručne greške
VL  - 71
IS  - 2
SP  - 107
EP  - 116
DO  - 10.2298/VETGL170322009D
ER  - 

@article{
author = "Đurić, Miloje and Jovanović, Ljubomir and Aničić, Milan and Nedić, Svetlana and Nedić, Sreten",
year = "2017",
abstract = "An apathetic 14-year-old Hungarian Puli bitch was presented with a seven day history of vaginal discharge and reported ovariohysterectomy (OHE) conducted six years ago. Clinical examination showed elevated body temperature (39.5°C), enlarged abdomen, and vulvar swelling. Despite a reported OHE in the anamnesis, ultrasound examination of the abdomen demonstrated the presence of both ovaries and a large anechogenic zone resembling the uterus. Vaginal cytologic examination and hormonal analysis indicated that the bitch was in the oestrus phase of the cycle. After laparotomy, both intact ovaries and an enlarged uterus with marked adhesions and filled with liquid content were visualised. The cervix was situated in the caudal part of the abdominal cavity and connected to the uterus by only an adhesion stump. After extirpation, uterus and ovarian tissue samples were sent for histological examination. Histological findings of uterus and ovarian tissue samples indicated a diagnosis of mucometra. Obviously, OHE was not conducted professionally, and the procedure performed only prevented conception, but ovaries and uterus continued to be active and caused a serious health disorder after six years. When only a part of the ovaries or uterus is left after OHE, health complications can appear up to ten years later. Our case testifies that even if both ovaries and the entire uterus are left after OHE without communication with cervix and vagina, a bitch can live without noticeable health disorders up to six years. This underlines the importance of lymphatic drainage and resorption processes in the uterus as well as evacuation of uterus content through the vagina., Četrnaest godina stara kuja rase mađarski puli je dovedena u apatičnom stanju i sa anamnezom iscedka iz vagine koji je prisutan 7 dana. Prema rečima vlasnika ovariohisterektomija je urađena pre 6 godina. Kliničkim nalazom je utvrđena povišena telesna temperatura (39,5˚C) uvećan abdomen i otok vulve. Uprkos podacima iz anamneze ultrazvučni pregled je ukazivao na prisustvo oba jajnika i velike anehogene zone koja je ukazivala na prisustvo materice. Citološki nalaz brisa vagine i hormonska analiza su ukazivali na to da je kuja bila u estralnoj fazi ciklusa. Nakon laparotomije potvrđeno je prisustvo oba jajnika i uvećane materice koja je bila ispunjena tečnim sadržajem i sa velikim brojem adhezija. Cerviks je bio lociran u kaudalnom delu abdomena i pričvršćen za uterus samo adhezionim patrljkom. Nakon ekstirpacije uterus i ovarijumi su poslati na histološku analizu, kojom je utvrđena dijagnoza mukometre. Očigledno da prethodno urađena ovariohisterektomija nije bila obavljena stručno i da je urađeni postupak sprečio koncepciju ali su ovarijumi i materica nastavili da budu aktivni što je prouzrokovalo ozbiljan zdravstveni problem 6 godina kasnije. Kada nakon ovariohisterektomije ostanu samo delovi tkiva ovarijuma i uterusa zdravstvene komplikacije se mogu javiti i do 10 godina nakon operacije. Prikazani slučaj svedoči o tome da je, čak i u slučaju kada su nakon operacije ostavljeni oba ovarijuma i uterus bez komunikacije sa cerviksom i vaginom, kuja 6 godina živela bez ozbiljnijih zdravstvenih problema. Ovo naglašava značaj limfne drenaže i procesa resorpcije sadržaja iz materice, pored pražnjenja kroz cerviks i vaginu.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Mucometra in a bitch as a consequence of a serious professional mistake, Mukometra kao posledica ozbiljne stručne greške",
volume = "71",
number = "2",
pages = "107-116",
doi = "10.2298/VETGL170322009D"
}

Đurić, M., Jovanović, L., Aničić, M., Nedić, S.,& Nedić, S.. (2017). Mucometra in a bitch as a consequence of a serious professional mistake. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(2), 107-116.
https://doi.org/10.2298/VETGL170322009D

Đurić M, Jovanović L, Aničić M, Nedić S, Nedić S. Mucometra in a bitch as a consequence of a serious professional mistake. in Veterinarski Glasnik. 2017;71(2):107-116.
doi:10.2298/VETGL170322009D .

Đurić, Miloje, Jovanović, Ljubomir, Aničić, Milan, Nedić, Svetlana, Nedić, Sreten, "Mucometra in a bitch as a consequence of a serious professional mistake" in Veterinarski Glasnik, 71, no. 2 (2017):107-116,
https://doi.org/10.2298/VETGL170322009D . .

TY  - CONF
AU  - Maletić, Milan
AU  - Đukić, Božidar
AU  - Nedić, Svetlana
AU  - Stanišić, Ljubodrag
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Vakanjac, Slobodanka
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3825
AB  - Mastitis is the most common disease in dairy cattle and presents the most costly disorder
to the dairy industry. Subclinical mastitis is inflammation of the mammary gland that does
not create visible changes in the milk or the udder, thus, subclinically infected cows will
produce less milk, and the quality of the milk will be reduced. In addition, infected cows
can be a source of infection to other animals in the herd. Among other unspecified causative
agents of mastitis, algae Prototheca spp. is not included in routine diagnosis in laboratories.
However, Prototheca spp infections usually result in a chronic subclinical or mild clinical,
inflammatory process in the udder, and were followed by a dramatic loss in milk production
and a permanent increase in somatic cell count. This type of infection is rare, but the
incidence of reported cases is increasing worldwide. In our investigation, 605 milk samples
were obtained fromdairy cows in a total of 5 herds with a history of increasing somatic cell
counts, subclinical and mild clinical signs of udder infection, and/or unsuccessful response
to the usual therapy. Microbiological isolation of algae on Blood agar and Sabouraud agar
at 25°C and 37°C, respectively, showed that 39 (6.45%) samples were positive on
Prototheca spp. which was confirmed microscopically. The SCC of Prototheca spp.
positive milk samples were from 5×105
/mL up to 13 ×106
/mL. Considering the wide
distribution of these algae as saprophytes in the environment, further investigation should
include detail microbiological and molecular techniques in order to reveal genotypes
involved prevent infections and decrease economic losses.
PB  - Belgrade : Faculty of Agriculture
C3  - International Symposium on Animal Science (ISAS), Belgrade, 24 - 25 November, 2016
T1  - Bovine subclinical mastitis associated with Prototheca spp.
SP  - 327
EP  - 333
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3825
ER  - 

@conference{
author = "Maletić, Milan and Đukić, Božidar and Nedić, Svetlana and Stanišić, Ljubodrag and Stanimirović, Zoran and Stevanović, Jevrosima and Vakanjac, Slobodanka",
year = "2016",
abstract = "Mastitis is the most common disease in dairy cattle and presents the most costly disorder
to the dairy industry. Subclinical mastitis is inflammation of the mammary gland that does
not create visible changes in the milk or the udder, thus, subclinically infected cows will
produce less milk, and the quality of the milk will be reduced. In addition, infected cows
can be a source of infection to other animals in the herd. Among other unspecified causative
agents of mastitis, algae Prototheca spp. is not included in routine diagnosis in laboratories.
However, Prototheca spp infections usually result in a chronic subclinical or mild clinical,
inflammatory process in the udder, and were followed by a dramatic loss in milk production
and a permanent increase in somatic cell count. This type of infection is rare, but the
incidence of reported cases is increasing worldwide. In our investigation, 605 milk samples
were obtained fromdairy cows in a total of 5 herds with a history of increasing somatic cell
counts, subclinical and mild clinical signs of udder infection, and/or unsuccessful response
to the usual therapy. Microbiological isolation of algae on Blood agar and Sabouraud agar
at 25°C and 37°C, respectively, showed that 39 (6.45%) samples were positive on
Prototheca spp. which was confirmed microscopically. The SCC of Prototheca spp.
positive milk samples were from 5×105
/mL up to 13 ×106
/mL. Considering the wide
distribution of these algae as saprophytes in the environment, further investigation should
include detail microbiological and molecular techniques in order to reveal genotypes
involved prevent infections and decrease economic losses.",
publisher = "Belgrade : Faculty of Agriculture",
journal = "International Symposium on Animal Science (ISAS), Belgrade, 24 - 25 November, 2016",
title = "Bovine subclinical mastitis associated with Prototheca spp.",
pages = "327-333",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3825"
}

Maletić, M., Đukić, B., Nedić, S., Stanišić, L., Stanimirović, Z., Stevanović, J.,& Vakanjac, S.. (2016). Bovine subclinical mastitis associated with Prototheca spp.. in International Symposium on Animal Science (ISAS), Belgrade, 24 - 25 November, 2016
Belgrade : Faculty of Agriculture., 327-333.
https://hdl.handle.net/21.15107/rcub_veterinar_3825

Maletić M, Đukić B, Nedić S, Stanišić L, Stanimirović Z, Stevanović J, Vakanjac S. Bovine subclinical mastitis associated with Prototheca spp.. in International Symposium on Animal Science (ISAS), Belgrade, 24 - 25 November, 2016. 2016;:327-333.
https://hdl.handle.net/21.15107/rcub_veterinar_3825 .

Maletić, Milan, Đukić, Božidar, Nedić, Svetlana, Stanišić, Ljubodrag, Stanimirović, Zoran, Stevanović, Jevrosima, Vakanjac, Slobodanka, "Bovine subclinical mastitis associated with Prototheca spp." in International Symposium on Animal Science (ISAS), Belgrade, 24 - 25 November, 2016 (2016):327-333,
https://hdl.handle.net/21.15107/rcub_veterinar_3825 .

TY  - JOUR
AU  - Maletić, Milan
AU  - Vakanjac, Slobodanka
AU  - Nedić, Svetlana
AU  - Magaš, Vladimir
AU  - Đurić, Miloje
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1283
AB  - Microbiological examination of semen as part of health control of bulls is of great importance for the success cattle reproduction. Deep-frozen semen ofbulls should be free of pathogenic and conditionally pathogenic microorganisms and controlled production of seed should allow out. According to the OIE recommendations unfrozen dose of semen should not contain more than 5000 cfu / ml of saprophytic microorganisms. According to the Law of Veterinary Medicine of Serbia, Article 115 (Traffic reproductive material) it is prohibited to trade, import and export of seeds for artificial insemination, ova and fertilized eggs containing pathogens of animals or more bacteria than allowed, or that their biochemical, biophysical and morphological properties are not eligible for reproduction. Clear limits of number and types of allowed saprophytic microorganisms in deep- frozen semen is not listed. Microbiological analysis included a sample of 351 semen ofbulls in pure culture was isolated Candida albicans in 9 samples, Citrobacter freundii in 5 samples, while other samples were free of microorganisms. Infection with Candida albicans are well described in the literature, and it is known that can cause mastitis, endometritis and abortion in cows. Citrobacter freundii is known as a cause of nosocomial infection of respiratory, urinary tract and blood in humans. It was stated that 29% of opportunistic infections in humans are caused by these microorganisms. In veterinary medicine, infection with Citrobacter freundii have been described in dogs, laboratory animals, fish and turtles. It is known that Citrobacter species can cause abortions and mastitis in cows, but literature data on the effect of pathogen Citrobacter freundii on cows reproductive health are low. Isolation of this organism from the semen ofbulls has not been described in the available literature. On the basis of our recommendations and OIE Veterinary Act, and the little available literature on infections (especially C freundii) it is recommended that the deep-frozen bull semen is unusable for arteficial insemination.
AB  - Mikrobiološki pregled semena kao deo zdravstvene kontrole bikova od velikog je zna;aja za uspeh reprodukcije goveda. Duboko zamrznuto seme bikova treba da bude slobodno od patogenih, kao i od uslovno patogenih mikroorganizama, što kontrolisana proizvodnja semena treba da omogući. Prema OIE preporukama otopljena doza semena ne treba da sadrži više od 5000 cfu/ml saprofitskih mikroorganizama. Prema Zakonu o veterinarstvu Republike Srbije, članu 115 (Promet reproduktivnog materijala) “Zabranjen je promet, uvoz ili izvoz semena za veštačko osemenjavanje, jajnih ćelija i oplođenih jajnih ćelija koji sadrži uzročnike bolesti životinja ili veći broj bakterija od dozvoljenog ili koji svojim biohemijskim, biofizičkim i morfološkim svojstvima ne ispunjavaju uslove za reprodukciju”. Jasna granica broja i vrste dozvoljenih saprofitskih mikroorganizama u duboko zamrznutom semenu nije navedena. Mikrobiološkom analizom obuhvaćen je 351 uzorak duboko zamrznutog semena bikova, u čistoj kulturi je izolovana Candida albicans u 9 uzorka, Citrobacter freundii u 5 uzoraka, dok su ostali uzorci bili slobodni od mikroorganizama. Infekcije krava sa Candida albicans su dosta opisane u literaturi, i poznato je da može da izazove mastitise, endometritise i pobačaje kod krava. Citrobacter freundii je poznat kao uzročnik nozokomijalnih infekcija respiratornog, urinarnog trakta i krvi ljudi. Navedeno je da je 29% oportunističkih infekcija kod ljudi izazvano ovim mikroorganizmom. U veterinarskoj medicini infekcije sa Citrobacter freundii su opisane kod pasa, laboratorijskih životinja, riba i kornjača. Poznato je da Citrobacter vrste mogu izazvati mastitise i pobačaje krava, ali literaturni podaci o patogenom delovanju Citrobacter freundii na reproduktivno zdravlje krava su oskudni.Izolacija ovog mikroorganizma iz duboko zamrznutog semena bikova nije opisana u nama dostupnoj literaturi. Na osnovu preporuke OIE i našeg Zakona o veterinarstvu, a zbog malo dostupne literature o infekcijama (posebno C. freundii) data je preporuka, da je duboko zamrznuto seme bika neupotrebljivo za veštačko osemenjavanje.
PB  - Institut PKB Agroekonomik, Padinska skela
T2  - Zbornik naučnih radova Instituta PKB Agroekonomik
T1  - Isolation of Candida albicans and Citrobacter freundii from deep frozen semen of bulls
T1  - Izolacija Candide albicans i Citrobacter freundii iz duboko zamrznutog semena bika
VL  - 21
IS  - 3-4
SP  - 81
EP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1283
ER  - 

@article{
author = "Maletić, Milan and Vakanjac, Slobodanka and Nedić, Svetlana and Magaš, Vladimir and Đurić, Miloje",
year = "2015",
abstract = "Microbiological examination of semen as part of health control of bulls is of great importance for the success cattle reproduction. Deep-frozen semen ofbulls should be free of pathogenic and conditionally pathogenic microorganisms and controlled production of seed should allow out. According to the OIE recommendations unfrozen dose of semen should not contain more than 5000 cfu / ml of saprophytic microorganisms. According to the Law of Veterinary Medicine of Serbia, Article 115 (Traffic reproductive material) it is prohibited to trade, import and export of seeds for artificial insemination, ova and fertilized eggs containing pathogens of animals or more bacteria than allowed, or that their biochemical, biophysical and morphological properties are not eligible for reproduction. Clear limits of number and types of allowed saprophytic microorganisms in deep- frozen semen is not listed. Microbiological analysis included a sample of 351 semen ofbulls in pure culture was isolated Candida albicans in 9 samples, Citrobacter freundii in 5 samples, while other samples were free of microorganisms. Infection with Candida albicans are well described in the literature, and it is known that can cause mastitis, endometritis and abortion in cows. Citrobacter freundii is known as a cause of nosocomial infection of respiratory, urinary tract and blood in humans. It was stated that 29% of opportunistic infections in humans are caused by these microorganisms. In veterinary medicine, infection with Citrobacter freundii have been described in dogs, laboratory animals, fish and turtles. It is known that Citrobacter species can cause abortions and mastitis in cows, but literature data on the effect of pathogen Citrobacter freundii on cows reproductive health are low. Isolation of this organism from the semen ofbulls has not been described in the available literature. On the basis of our recommendations and OIE Veterinary Act, and the little available literature on infections (especially C freundii) it is recommended that the deep-frozen bull semen is unusable for arteficial insemination., Mikrobiološki pregled semena kao deo zdravstvene kontrole bikova od velikog je zna;aja za uspeh reprodukcije goveda. Duboko zamrznuto seme bikova treba da bude slobodno od patogenih, kao i od uslovno patogenih mikroorganizama, što kontrolisana proizvodnja semena treba da omogući. Prema OIE preporukama otopljena doza semena ne treba da sadrži više od 5000 cfu/ml saprofitskih mikroorganizama. Prema Zakonu o veterinarstvu Republike Srbije, članu 115 (Promet reproduktivnog materijala) “Zabranjen je promet, uvoz ili izvoz semena za veštačko osemenjavanje, jajnih ćelija i oplođenih jajnih ćelija koji sadrži uzročnike bolesti životinja ili veći broj bakterija od dozvoljenog ili koji svojim biohemijskim, biofizičkim i morfološkim svojstvima ne ispunjavaju uslove za reprodukciju”. Jasna granica broja i vrste dozvoljenih saprofitskih mikroorganizama u duboko zamrznutom semenu nije navedena. Mikrobiološkom analizom obuhvaćen je 351 uzorak duboko zamrznutog semena bikova, u čistoj kulturi je izolovana Candida albicans u 9 uzorka, Citrobacter freundii u 5 uzoraka, dok su ostali uzorci bili slobodni od mikroorganizama. Infekcije krava sa Candida albicans su dosta opisane u literaturi, i poznato je da može da izazove mastitise, endometritise i pobačaje kod krava. Citrobacter freundii je poznat kao uzročnik nozokomijalnih infekcija respiratornog, urinarnog trakta i krvi ljudi. Navedeno je da je 29% oportunističkih infekcija kod ljudi izazvano ovim mikroorganizmom. U veterinarskoj medicini infekcije sa Citrobacter freundii su opisane kod pasa, laboratorijskih životinja, riba i kornjača. Poznato je da Citrobacter vrste mogu izazvati mastitise i pobačaje krava, ali literaturni podaci o patogenom delovanju Citrobacter freundii na reproduktivno zdravlje krava su oskudni.Izolacija ovog mikroorganizma iz duboko zamrznutog semena bikova nije opisana u nama dostupnoj literaturi. Na osnovu preporuke OIE i našeg Zakona o veterinarstvu, a zbog malo dostupne literature o infekcijama (posebno C. freundii) data je preporuka, da je duboko zamrznuto seme bika neupotrebljivo za veštačko osemenjavanje.",
publisher = "Institut PKB Agroekonomik, Padinska skela",
journal = "Zbornik naučnih radova Instituta PKB Agroekonomik",
title = "Isolation of Candida albicans and Citrobacter freundii from deep frozen semen of bulls, Izolacija Candide albicans i Citrobacter freundii iz duboko zamrznutog semena bika",
volume = "21",
number = "3-4",
pages = "81-86",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1283"
}

Maletić, M., Vakanjac, S., Nedić, S., Magaš, V.,& Đurić, M.. (2015). Isolation of Candida albicans and Citrobacter freundii from deep frozen semen of bulls. in Zbornik naučnih radova Instituta PKB Agroekonomik
Institut PKB Agroekonomik, Padinska skela., 21(3-4), 81-86.
https://hdl.handle.net/21.15107/rcub_veterinar_1283

Maletić M, Vakanjac S, Nedić S, Magaš V, Đurić M. Isolation of Candida albicans and Citrobacter freundii from deep frozen semen of bulls. in Zbornik naučnih radova Instituta PKB Agroekonomik. 2015;21(3-4):81-86.
https://hdl.handle.net/21.15107/rcub_veterinar_1283 .

Maletić, Milan, Vakanjac, Slobodanka, Nedić, Svetlana, Magaš, Vladimir, Đurić, Miloje, "Isolation of Candida albicans and Citrobacter freundii from deep frozen semen of bulls" in Zbornik naučnih radova Instituta PKB Agroekonomik, 21, no. 3-4 (2015):81-86,
https://hdl.handle.net/21.15107/rcub_veterinar_1283 .

TY  - JOUR
AU  - Vakanjac, Slobodanka
AU  - Pavlović, Vojislav
AU  - Magaš, Vladimir
AU  - Pavlović, Miloš
AU  - Đurić, Miloje
AU  - Maletić, Milan
AU  - Nedić, Svetlana
AU  - Sočo, Ivan
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/959
AB  - Inflammation of the mammary gland, mastitis in cows, presents one of the most acute problems in intensive dairy production, inflicting huge economic losses. In the course of one year, 80 samples were taken at investigated farms from udder quarters of cows with clinical mastitis and 160 samples from udder quarters of cows with subclinical mastitis. The efficacy of three preparations, A, B, and C, was examined in the treatment of clinical and subclinical mastitis in cows. The investigations indicate that antibiotic preparation A (neomycin, polimixine B, oleandomycin and prednisolone) exhibited a greater efficacy in the treatment of clinical mastitis caused by Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis and Micrococcus sp., but a smaller efficacy in the treatment of subclinical mastitis caused by Staphylococcus aureus. Preparation B (amoxicillin, clavulanic acid and prednisolone) exhibited a higher efficacy in the treatment of clinical mastitis caused by Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis and Micrococcus, but a weaker effect in the treatment of subclinical mastitis caused by Staphylococcus aureus. Preparation C (procaine penicillin G, streptomycin, neomycin sulfate and prednisolone acetate) exihibited efficacy in the treatment of clinical and subclinical mastitis caused by Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis, Micrococcus, Staphylococcus aureus and Esherichie coli.
AB  - Zapaljenje mlečne žlezde (mastitis) kod krava predstavlja jedan od najaktuelnijih problema u intenzivnoj proizvodnji mleka, nanoseći velike ekonomske gubitke. Sa ispitivanih farmi u toku jedne godine, uzeto je 80 uzoraka mleka iz četvrti vimena krava sa kliničkim mastitisom i 160 uzoraka mleka iz četvrti vimena krava sa supkliničkim mastitisom. Ispitana je efikasnost tri preparata - A, B i C, u lečenju krava od kliničkih i supkliničkih mastitisa. Preparat A (neomicin, polimiksin B, oleandomicin i prednizolon) pokazao je veću efikasnost u lečenju kliničkih mastitisa izazvanih bakterijama Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis i Micrococcus sp., ali slabiju efikasnost u lečenju supkliničkih mastitisa izazvanih Staphylococcus aureus. Preparat B (amoksicilin, klavulanska kiselina i prednizolon) pokazao je veću efikasnost u lečenju kliničkih mastitisa izazvanih bakterijama Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis i Micrococcus, ali slabije dejstvo u lečenju supkliničkih mastitisa izazvanih bakterijom Staphylococcus aureus. Preparat C (prokain penicilin G, streptomicin, neomicin sulfat i prednizolon acetat) pokazao je efikasnost u lečenju kliničkih i supkliničkih mastitisa izazvanih bakterijama Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis, Micrococcus, Staphylococcus aureus i Esherichie coli.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Investigations of efficacy of intramammary applied antimicrobials and glucocorticosteroides in the treatment of subclinical and clinical mastitis in cows
T1  - Ispitivanje efikasnosti intramamarno aplikovanih antibiotika i glukokortikosteroida u lečenju supkliničkih i kliničkih mastitisa kod krava
VL  - 67
IS  - 1-2
SP  - 15
EP  - 27
DO  - 10.2298/VETGL1302015V
ER  - 

@article{
author = "Vakanjac, Slobodanka and Pavlović, Vojislav and Magaš, Vladimir and Pavlović, Miloš and Đurić, Miloje and Maletić, Milan and Nedić, Svetlana and Sočo, Ivan",
year = "2013",
abstract = "Inflammation of the mammary gland, mastitis in cows, presents one of the most acute problems in intensive dairy production, inflicting huge economic losses. In the course of one year, 80 samples were taken at investigated farms from udder quarters of cows with clinical mastitis and 160 samples from udder quarters of cows with subclinical mastitis. The efficacy of three preparations, A, B, and C, was examined in the treatment of clinical and subclinical mastitis in cows. The investigations indicate that antibiotic preparation A (neomycin, polimixine B, oleandomycin and prednisolone) exhibited a greater efficacy in the treatment of clinical mastitis caused by Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis and Micrococcus sp., but a smaller efficacy in the treatment of subclinical mastitis caused by Staphylococcus aureus. Preparation B (amoxicillin, clavulanic acid and prednisolone) exhibited a higher efficacy in the treatment of clinical mastitis caused by Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis and Micrococcus, but a weaker effect in the treatment of subclinical mastitis caused by Staphylococcus aureus. Preparation C (procaine penicillin G, streptomycin, neomycin sulfate and prednisolone acetate) exihibited efficacy in the treatment of clinical and subclinical mastitis caused by Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis, Micrococcus, Staphylococcus aureus and Esherichie coli., Zapaljenje mlečne žlezde (mastitis) kod krava predstavlja jedan od najaktuelnijih problema u intenzivnoj proizvodnji mleka, nanoseći velike ekonomske gubitke. Sa ispitivanih farmi u toku jedne godine, uzeto je 80 uzoraka mleka iz četvrti vimena krava sa kliničkim mastitisom i 160 uzoraka mleka iz četvrti vimena krava sa supkliničkim mastitisom. Ispitana je efikasnost tri preparata - A, B i C, u lečenju krava od kliničkih i supkliničkih mastitisa. Preparat A (neomicin, polimiksin B, oleandomicin i prednizolon) pokazao je veću efikasnost u lečenju kliničkih mastitisa izazvanih bakterijama Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis i Micrococcus sp., ali slabiju efikasnost u lečenju supkliničkih mastitisa izazvanih Staphylococcus aureus. Preparat B (amoksicilin, klavulanska kiselina i prednizolon) pokazao je veću efikasnost u lečenju kliničkih mastitisa izazvanih bakterijama Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis i Micrococcus, ali slabije dejstvo u lečenju supkliničkih mastitisa izazvanih bakterijom Staphylococcus aureus. Preparat C (prokain penicilin G, streptomicin, neomicin sulfat i prednizolon acetat) pokazao je efikasnost u lečenju kliničkih i supkliničkih mastitisa izazvanih bakterijama Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis, Micrococcus, Staphylococcus aureus i Esherichie coli.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Investigations of efficacy of intramammary applied antimicrobials and glucocorticosteroides in the treatment of subclinical and clinical mastitis in cows, Ispitivanje efikasnosti intramamarno aplikovanih antibiotika i glukokortikosteroida u lečenju supkliničkih i kliničkih mastitisa kod krava",
volume = "67",
number = "1-2",
pages = "15-27",
doi = "10.2298/VETGL1302015V"
}

Vakanjac, S., Pavlović, V., Magaš, V., Pavlović, M., Đurić, M., Maletić, M., Nedić, S.,& Sočo, I.. (2013). Investigations of efficacy of intramammary applied antimicrobials and glucocorticosteroides in the treatment of subclinical and clinical mastitis in cows. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(1-2), 15-27.
https://doi.org/10.2298/VETGL1302015V

Vakanjac S, Pavlović V, Magaš V, Pavlović M, Đurić M, Maletić M, Nedić S, Sočo I. Investigations of efficacy of intramammary applied antimicrobials and glucocorticosteroides in the treatment of subclinical and clinical mastitis in cows. in Veterinarski Glasnik. 2013;67(1-2):15-27.
doi:10.2298/VETGL1302015V .

Vakanjac, Slobodanka, Pavlović, Vojislav, Magaš, Vladimir, Pavlović, Miloš, Đurić, Miloje, Maletić, Milan, Nedić, Svetlana, Sočo, Ivan, "Investigations of efficacy of intramammary applied antimicrobials and glucocorticosteroides in the treatment of subclinical and clinical mastitis in cows" in Veterinarski Glasnik, 67, no. 1-2 (2013):15-27,
https://doi.org/10.2298/VETGL1302015V . .

TY  - JOUR
AU  - Maletić, Milan
AU  - Vakanjac, Slobodanka
AU  - Đelić, Ninoslav
AU  - Lakić, Nada
AU  - Pavlović, Miloš
AU  - Nedić, Svetlana
AU  - Stanimirović, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1057
AB  - Lactoferrin (LTF) is a glycoprotein, a member of transferrin gene family which plays an important role in immune mechanisms in the mammary glands of cows. The amount of lactoferrin increases during inflammatory processes and viral infections. The aim of this investigation was to monitor the distribution of lactoferrin gene genotypes and its connection to milk quality and the occurrence of mammary gland diseases in 46 Holstein-Freisian cows of different age (2-7 years) on a farm near Belgrade. DNA was isolated from blood samples, and the polymorphism of lactoferrin gene was deterimined by PCR-RFLP method using the restriction enzyme Eco RI. We found two alelic forms of this gene in cows included in these experiments (A and B) and two genotypes (AA and AB) in a ratio 71.7% to 28.3%. The genotype BB was not found in this sample. In order to determine the degree of differences between genotypes we used discriminant analysis which has shown that there is a statistically significant difference between genotypes AA and AB with respect to productive parameters. When analyzed separately, the only parameter which differed significantly (p=0.021) between two genotypes was total milk production. Individuals with observed genotypes are most similar for the amount of milk fat (p=0.271). There is no statistically significant difference in the number of somatic cells in milk samples between the examined genotypes.
AB  - Laktoferin (LTF) je glikoprotein, član familije transferina i igra važnu ulogu u odbrambenom mehanizmu mlečne žlezde krava. Količina cartoferina raste tokom inflamatornog procesa i virusne infekcije. Cilj ovog rada je bilo praćenje distribucije genotipova laktoferin gena i njihova povezanost sa kvalitetom mleka i pojavom oboljenja mlečne žlezde kod 46 krava Holštajn-Frizijske rase, različite starosti (2-7 godina) na farmi u blizini Beograda. Obavljena je izolacija DNK iz krvi, a polimorfizam laktoferin gena utvrđen je PCR-RFLP metodom pomoću restrikcionog enzima Eco RI. Kod životinja uključenih u ogled utvrđene su dve alelne forme, A i B i dva genotipa, AA i AB u odnosu 71,74% prema 28,26%. Genotip BB nije pronađen u datom uzorku životinja. U cilju utvrđivanja stepena razdvajanja genotipova primenjena je diskriminaciona analiza koja je ukazala da se genotipovi AA i AB statistički značajno razlikuju prema proizvodnim parametrima posmatranim istovremeno. Pojedinačno, samo se po parametru ukupna proizvodnja mleka, krava analiziranih genotipova statistički značajno razlikuju (p=0.021). Grla posmatranih genotipova su najsličnija po količini mlečne masti (p=0.271). Nije ustanovljena statistički značajna razlika u broju somatskih ćelija u mleku između ispitivanih genotipova.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Analysis of lactoferin gene polymophism and its association to milk quality and mammary gland health in Holstein-Friesian cows
T1  - Analiza polimorfizma laktoferin gena i njegova povezanost sa kvalitetom mleka i zdravljem mlečne žlezde kod Holštajn-Frizijskih krava
VL  - 63
IS  - 5-6
SP  - 487
EP  - 498
DO  - 10.2298/AVB1306487M
ER  - 

@article{
author = "Maletić, Milan and Vakanjac, Slobodanka and Đelić, Ninoslav and Lakić, Nada and Pavlović, Miloš and Nedić, Svetlana and Stanimirović, Zoran",
year = "2013",
abstract = "Lactoferrin (LTF) is a glycoprotein, a member of transferrin gene family which plays an important role in immune mechanisms in the mammary glands of cows. The amount of lactoferrin increases during inflammatory processes and viral infections. The aim of this investigation was to monitor the distribution of lactoferrin gene genotypes and its connection to milk quality and the occurrence of mammary gland diseases in 46 Holstein-Freisian cows of different age (2-7 years) on a farm near Belgrade. DNA was isolated from blood samples, and the polymorphism of lactoferrin gene was deterimined by PCR-RFLP method using the restriction enzyme Eco RI. We found two alelic forms of this gene in cows included in these experiments (A and B) and two genotypes (AA and AB) in a ratio 71.7% to 28.3%. The genotype BB was not found in this sample. In order to determine the degree of differences between genotypes we used discriminant analysis which has shown that there is a statistically significant difference between genotypes AA and AB with respect to productive parameters. When analyzed separately, the only parameter which differed significantly (p=0.021) between two genotypes was total milk production. Individuals with observed genotypes are most similar for the amount of milk fat (p=0.271). There is no statistically significant difference in the number of somatic cells in milk samples between the examined genotypes., Laktoferin (LTF) je glikoprotein, član familije transferina i igra važnu ulogu u odbrambenom mehanizmu mlečne žlezde krava. Količina cartoferina raste tokom inflamatornog procesa i virusne infekcije. Cilj ovog rada je bilo praćenje distribucije genotipova laktoferin gena i njihova povezanost sa kvalitetom mleka i pojavom oboljenja mlečne žlezde kod 46 krava Holštajn-Frizijske rase, različite starosti (2-7 godina) na farmi u blizini Beograda. Obavljena je izolacija DNK iz krvi, a polimorfizam laktoferin gena utvrđen je PCR-RFLP metodom pomoću restrikcionog enzima Eco RI. Kod životinja uključenih u ogled utvrđene su dve alelne forme, A i B i dva genotipa, AA i AB u odnosu 71,74% prema 28,26%. Genotip BB nije pronađen u datom uzorku životinja. U cilju utvrđivanja stepena razdvajanja genotipova primenjena je diskriminaciona analiza koja je ukazala da se genotipovi AA i AB statistički značajno razlikuju prema proizvodnim parametrima posmatranim istovremeno. Pojedinačno, samo se po parametru ukupna proizvodnja mleka, krava analiziranih genotipova statistički značajno razlikuju (p=0.021). Grla posmatranih genotipova su najsličnija po količini mlečne masti (p=0.271). Nije ustanovljena statistički značajna razlika u broju somatskih ćelija u mleku između ispitivanih genotipova.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Analysis of lactoferin gene polymophism and its association to milk quality and mammary gland health in Holstein-Friesian cows, Analiza polimorfizma laktoferin gena i njegova povezanost sa kvalitetom mleka i zdravljem mlečne žlezde kod Holštajn-Frizijskih krava",
volume = "63",
number = "5-6",
pages = "487-498",
doi = "10.2298/AVB1306487M"
}

Maletić, M., Vakanjac, S., Đelić, N., Lakić, N., Pavlović, M., Nedić, S.,& Stanimirović, Z.. (2013). Analysis of lactoferin gene polymophism and its association to milk quality and mammary gland health in Holstein-Friesian cows. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(5-6), 487-498.
https://doi.org/10.2298/AVB1306487M

Maletić M, Vakanjac S, Đelić N, Lakić N, Pavlović M, Nedić S, Stanimirović Z. Analysis of lactoferin gene polymophism and its association to milk quality and mammary gland health in Holstein-Friesian cows. in Acta Veterinaria-Beograd. 2013;63(5-6):487-498.
doi:10.2298/AVB1306487M .

Maletić, Milan, Vakanjac, Slobodanka, Đelić, Ninoslav, Lakić, Nada, Pavlović, Miloš, Nedić, Svetlana, Stanimirović, Zoran, "Analysis of lactoferin gene polymophism and its association to milk quality and mammary gland health in Holstein-Friesian cows" in Acta Veterinaria-Beograd, 63, no. 5-6 (2013):487-498,
https://doi.org/10.2298/AVB1306487M . .

TY  - JOUR
AU  - Magaš, Vladimir
AU  - Vakanjac, Slobodanka
AU  - Pavlović, V.
AU  - Velebit, Branko
AU  - Mirilović, Milorad
AU  - Maletić, Milan
AU  - Đurić, M.
AU  - Nedić, Svetlana
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1060
AB  - Mastitis in cows represents one of the most actual problems in intensive dairy production. The longtime different approaches to the treatment of mastitis have not offered a suitable solution, and the problem of mastitis is still present and acute. Prevention of pathogen penetration into the mammary gland, its colonization and multiplication impose a constant need for regular inspections of milk, as well as preventive and therapeutic measures to reduce the incidence of mastitis. Studies in the field of vaccination of ruminants against mastitis pathogens suggest a limited success in obtaining significant results in immunoprophylaxis. Considering the results of other researchers and our own research, and bearing in mind the problems mastitis caused by Staphylococcus aureus and Streptococcus agalactiae represent, the aim of this study was the preparation and testing of an indigenous vaccine prepared with two stable strains, SAU 7 (S. aureus) and SAG 3 (Str.agalactiae). The dose was 5 mL/cow and consisted of inactivated bacterial S. aureus SAU 7 cells in a concentration of 1x1010 cfu/mL and Str. agalactiae SAG 3 in a concentration of 4 x 109 cfu/mL. The number of somatic cells in the milk samples during the whole study period was higher in vaccinated cows in both groups compared with the control, but this difference was not statistically significant. The concentration of immunoglobulin IgG in the milk of vaccinated cows was significantly higher than the concentration of this class of proteins in the milk of unvaccinated cows.
AB  - Upala mlečne žlezde ili mastitis krava, predstavlja jedan od najaktuelnijih problema u intenzivnoj proizvodnji mleka. Dugogodišnji različiti pristupi lečenju mastitisa nisu dali odgovarajuće rešenje, pa je problem mastitisa i dalje prisutan i aktuelan. Sprečavanje prodora patogenog uzročnika u mlečnu žlezdu, njegovo naseljavanje i razmnožavanje, nameću stalnu potrebu za redovnom kontrolom mleka, kao i preduzimanje preventivnih i terapijskih mera u cilju smanjenja pojave nastanka mastitisa. Preventivna mera je i terapija krava u zasušenju koja podrazumeva lokalnu aplikaciju antibiotika nakon poslednje muže. Terapija krava u zasušenju treba u narednoj laktaciji da obezbedi što duži period neinficiranosti vimena, a obavezna je kod dijagnostikovanih subkliničkih mastitisa izazvanih sa Staphylococcus aureus i Streptococcus agalactiae. Radovi iz oblasti vakcinisanja preživara protiv uzročnika mastitisa ukazuju na ograničen uspeh u dobijanju značajnijih rezultata imunoprofilakse. S obzirom na rezultate drugih istraživača i sopstvenih preliminarnih istraživanja, a imajuću u vidu problem koji predstavljaju mastitisi uzrokovani Staphylococcus aureusom i Streptococcus agalactiae, cilj istraživanja je bio priprema i ispitavanje autohtone vakcine pripremljene od dva štalska soja SAU 7 (S. aureus) i SAG 3 (Str. agalactiae). Broj somatskih ćelija u uzorcima mleka tokom celog trajanja ogleda je bio viši u obe ispitivane vakcinisane grupe, u odnosu na kontrolnu, ali nema statističke značajnosti. Koncentracija imunoglobulina IgG u mleku vakcinisanih krava je bila značajno povećana u odnosu na koncentraciju imunoglobulina nevakcinisane grupe.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Efficiency evaluation of a bivalent vaccine in the prophylaxis of mastitis in cows
T1  - Ocena efikasnosti bivalentne vakcine u profilaksi nastanka mastitisa kod krava
VL  - 63
IS  - 5-6
SP  - 525
EP  - 536
DO  - 10.2298/AVB1306525M
ER  - 

@article{
author = "Magaš, Vladimir and Vakanjac, Slobodanka and Pavlović, V. and Velebit, Branko and Mirilović, Milorad and Maletić, Milan and Đurić, M. and Nedić, Svetlana",
year = "2013",
abstract = "Mastitis in cows represents one of the most actual problems in intensive dairy production. The longtime different approaches to the treatment of mastitis have not offered a suitable solution, and the problem of mastitis is still present and acute. Prevention of pathogen penetration into the mammary gland, its colonization and multiplication impose a constant need for regular inspections of milk, as well as preventive and therapeutic measures to reduce the incidence of mastitis. Studies in the field of vaccination of ruminants against mastitis pathogens suggest a limited success in obtaining significant results in immunoprophylaxis. Considering the results of other researchers and our own research, and bearing in mind the problems mastitis caused by Staphylococcus aureus and Streptococcus agalactiae represent, the aim of this study was the preparation and testing of an indigenous vaccine prepared with two stable strains, SAU 7 (S. aureus) and SAG 3 (Str.agalactiae). The dose was 5 mL/cow and consisted of inactivated bacterial S. aureus SAU 7 cells in a concentration of 1x1010 cfu/mL and Str. agalactiae SAG 3 in a concentration of 4 x 109 cfu/mL. The number of somatic cells in the milk samples during the whole study period was higher in vaccinated cows in both groups compared with the control, but this difference was not statistically significant. The concentration of immunoglobulin IgG in the milk of vaccinated cows was significantly higher than the concentration of this class of proteins in the milk of unvaccinated cows., Upala mlečne žlezde ili mastitis krava, predstavlja jedan od najaktuelnijih problema u intenzivnoj proizvodnji mleka. Dugogodišnji različiti pristupi lečenju mastitisa nisu dali odgovarajuće rešenje, pa je problem mastitisa i dalje prisutan i aktuelan. Sprečavanje prodora patogenog uzročnika u mlečnu žlezdu, njegovo naseljavanje i razmnožavanje, nameću stalnu potrebu za redovnom kontrolom mleka, kao i preduzimanje preventivnih i terapijskih mera u cilju smanjenja pojave nastanka mastitisa. Preventivna mera je i terapija krava u zasušenju koja podrazumeva lokalnu aplikaciju antibiotika nakon poslednje muže. Terapija krava u zasušenju treba u narednoj laktaciji da obezbedi što duži period neinficiranosti vimena, a obavezna je kod dijagnostikovanih subkliničkih mastitisa izazvanih sa Staphylococcus aureus i Streptococcus agalactiae. Radovi iz oblasti vakcinisanja preživara protiv uzročnika mastitisa ukazuju na ograničen uspeh u dobijanju značajnijih rezultata imunoprofilakse. S obzirom na rezultate drugih istraživača i sopstvenih preliminarnih istraživanja, a imajuću u vidu problem koji predstavljaju mastitisi uzrokovani Staphylococcus aureusom i Streptococcus agalactiae, cilj istraživanja je bio priprema i ispitavanje autohtone vakcine pripremljene od dva štalska soja SAU 7 (S. aureus) i SAG 3 (Str. agalactiae). Broj somatskih ćelija u uzorcima mleka tokom celog trajanja ogleda je bio viši u obe ispitivane vakcinisane grupe, u odnosu na kontrolnu, ali nema statističke značajnosti. Koncentracija imunoglobulina IgG u mleku vakcinisanih krava je bila značajno povećana u odnosu na koncentraciju imunoglobulina nevakcinisane grupe.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Efficiency evaluation of a bivalent vaccine in the prophylaxis of mastitis in cows, Ocena efikasnosti bivalentne vakcine u profilaksi nastanka mastitisa kod krava",
volume = "63",
number = "5-6",
pages = "525-536",
doi = "10.2298/AVB1306525M"
}

Magaš, V., Vakanjac, S., Pavlović, V., Velebit, B., Mirilović, M., Maletić, M., Đurić, M.,& Nedić, S.. (2013). Efficiency evaluation of a bivalent vaccine in the prophylaxis of mastitis in cows. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(5-6), 525-536.
https://doi.org/10.2298/AVB1306525M

Magaš V, Vakanjac S, Pavlović V, Velebit B, Mirilović M, Maletić M, Đurić M, Nedić S. Efficiency evaluation of a bivalent vaccine in the prophylaxis of mastitis in cows. in Acta Veterinaria-Beograd. 2013;63(5-6):525-536.
doi:10.2298/AVB1306525M .

Magaš, Vladimir, Vakanjac, Slobodanka, Pavlović, V., Velebit, Branko, Mirilović, Milorad, Maletić, Milan, Đurić, M., Nedić, Svetlana, "Efficiency evaluation of a bivalent vaccine in the prophylaxis of mastitis in cows" in Acta Veterinaria-Beograd, 63, no. 5-6 (2013):525-536,
https://doi.org/10.2298/AVB1306525M . .