TY  - JOUR
AU  - Stojković, Maja
AU  - Todorović, Zoran
AU  - Protić, Dragana
AU  - Stevanović, Strahinja
AU  - Medić, Dragana
AU  - Charvet, Claude L.
AU  - Marjanović, Djordje
AU  - Nedeljković Trailović, Jelena
AU  - Trailović, Saša M.
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3564
AB  - The control of parasitic nematode infections relies mostly on anthelmintics. The potential pharmacotherapeutic application of phytochemicals, in order to overcome parasite resistance and enhance the effect of existing drugs, is becoming increasingly important. The antinematodal effects of carveol was tested on the free-living nematode Caenorhabditis elegans and the neuromuscular preparation of the parasitic nematode Ascaris suum. Carveol caused spastic and geraniol atonic paralysis in C. elegans. In A. suum carveol potentiated contractions induced by acetylcholine (ACh) and this effect was confirmed with two-electrode voltage-clamp electrophysiology on the A. suum nicotinic acetylcholine receptor expressed in Xenopus oocytes. However, potentiating effect of carveol on ACh-induced contractions was partially sensitive to atropine, indicates a dominant nicotine effect but also the involvement of some muscarinic structures. The effects of carveol on the neuromuscular system of mammals are also specific. In micromolar concentrations, carveol acts as a non-competitive ACh antagonist on ileum contractions. Unlike atropine, it does not change the EC50 of ACh but reduces the amplitude of contractions. Carveol caused an increase in Electrical Field Stimulation-evoked contractions of the isolated rat diaphragm, but at higher concentrations it caused an inhibition. Also, carveol neutralized the mecamylamine-induced tetanic fade, indicating a possibly different pre-and post-synaptic action at the neuromuscular junction.
PB  - Frontiers Media
T2  - Frontiers in Pharmacology
T1  - Pharmacological effects of monoterpene carveol on the neuromuscular system of nematodes and mammals
VL  - 15
DO  - 10.3389/fphar.2024.1326779
ER  - 

@article{
author = "Stojković, Maja and Todorović, Zoran and Protić, Dragana and Stevanović, Strahinja and Medić, Dragana and Charvet, Claude L. and Marjanović, Djordje and Nedeljković Trailović, Jelena and Trailović, Saša M.",
year = "2024",
abstract = "The control of parasitic nematode infections relies mostly on anthelmintics. The potential pharmacotherapeutic application of phytochemicals, in order to overcome parasite resistance and enhance the effect of existing drugs, is becoming increasingly important. The antinematodal effects of carveol was tested on the free-living nematode Caenorhabditis elegans and the neuromuscular preparation of the parasitic nematode Ascaris suum. Carveol caused spastic and geraniol atonic paralysis in C. elegans. In A. suum carveol potentiated contractions induced by acetylcholine (ACh) and this effect was confirmed with two-electrode voltage-clamp electrophysiology on the A. suum nicotinic acetylcholine receptor expressed in Xenopus oocytes. However, potentiating effect of carveol on ACh-induced contractions was partially sensitive to atropine, indicates a dominant nicotine effect but also the involvement of some muscarinic structures. The effects of carveol on the neuromuscular system of mammals are also specific. In micromolar concentrations, carveol acts as a non-competitive ACh antagonist on ileum contractions. Unlike atropine, it does not change the EC50 of ACh but reduces the amplitude of contractions. Carveol caused an increase in Electrical Field Stimulation-evoked contractions of the isolated rat diaphragm, but at higher concentrations it caused an inhibition. Also, carveol neutralized the mecamylamine-induced tetanic fade, indicating a possibly different pre-and post-synaptic action at the neuromuscular junction.",
publisher = "Frontiers Media",
journal = "Frontiers in Pharmacology",
title = "Pharmacological effects of monoterpene carveol on the neuromuscular system of nematodes and mammals",
volume = "15",
doi = "10.3389/fphar.2024.1326779"
}

Stojković, M., Todorović, Z., Protić, D., Stevanović, S., Medić, D., Charvet, C. L., Marjanović, D., Nedeljković Trailović, J.,& Trailović, S. M.. (2024). Pharmacological effects of monoterpene carveol on the neuromuscular system of nematodes and mammals. in Frontiers in Pharmacology
Frontiers Media., 15.
https://doi.org/10.3389/fphar.2024.1326779

Stojković M, Todorović Z, Protić D, Stevanović S, Medić D, Charvet CL, Marjanović D, Nedeljković Trailović J, Trailović SM. Pharmacological effects of monoterpene carveol on the neuromuscular system of nematodes and mammals. in Frontiers in Pharmacology. 2024;15.
doi:10.3389/fphar.2024.1326779 .

Stojković, Maja, Todorović, Zoran, Protić, Dragana, Stevanović, Strahinja, Medić, Dragana, Charvet, Claude L., Marjanović, Djordje, Nedeljković Trailović, Jelena, Trailović, Saša M., "Pharmacological effects of monoterpene carveol on the neuromuscular system of nematodes and mammals" in Frontiers in Pharmacology, 15 (2024),
https://doi.org/10.3389/fphar.2024.1326779 . .

TY  - CONF
AU  - Trailović, Saša M.
AU  - Milovanović, Mirjana
AU  - Marjanović, Đorđe S.
AU  - Medić, Dragana
AU  - Marinković, Darko
AU  - Aničić, Milan
AU  - Stojković, Maja
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3709
AB  - Parazitske nematode su značajni patogeni životinja i ljudi. Nažalost,
lekovi koji se koriste u farmakoterapiji helmintoza nisu apsolutno
efikasni i bezbedni protiv svih kombinacija vrsta parazita/domaćina.
Postoji jasna i hitna potreba za novim efikasnim antinematodnim jedinjenjima.
Ova potreba postaje sve važnija sa sve većim nivoom rezistencije
parazita na postojeće lekove. Većina anthelminika deluje na
neuromišićni sistem nematoda. Mi u projektu predviđamo da se dalje
ispita neuromišićni sistem na modelima parazitske nematode Ascaris
suum i slobodnoživuće nematode Caenorhabditis elegans. Fokusiraćemo
se na ispitivanje kombinaciju novih (muskarinski i serotoninski
receptori) i prethodno potvrđenih potencijalnih ciljnih mesta (jonotropni
nACh i GABA receptori). Predlažemo da se odredi lokalizacija ovih
receptora u somatskim mišićima A. suum metodom RNA-scope in situ
hibridizacije. Farmakološki ćemo okarakterisati ove receptore koristeći
kombinaciju studija pokretljivosti, testova faringijalnog pumpanja
(C. elegans), testova kontrakcije nervnomišićnog preparata (A. suum)
i elektrofiziološkim metodama “voltage“ i „current clamp“ sa dve elektrode
(A. suum). Koristeći ove tehnike, ispitivaćemo sledeće: 1. Efikasnost
kombinacija postojećih GABA-ergičnih i holinergičkih lekova u
cilju poboljšanja efikasnosti; 2. Potencijalno nove mete za anthelmintike
(muskarinski ACh receptor i serotonergički MOD 1 receptor); 3)
Prenamenu postojećih lekova koji ispoljavaju potencijalna anthelmintička
svojstva; 4) Antinematodna svojstva terpenoidnih aktivnih sastojaka
(AI) biljnih esencijalnih ulja i potencijal kombinacija AI i postojećih
lekova. Očekujemo da će naši rezultati pružiti precizniju karakterizaciju postojećih (holinergičkih i GABA-ergičnih) i potencijalnih (muskarinskih
i seretonergičkih) receptora za antiparazitske lekove. Utvrdićemo
da li neki stariji lekovi imaju potencijal da se ponovo koriste kao anthelmintici,
samostalno ili u kombinacijama.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024
T1  - Prezentacija projekta Programa Prizma 2023 Fonda za nauku Republike Srbije: Proučavanje ciljnih mesta delovanja antihelmintika u neuromuskularnom sistemu parazitskih nematoda u cilju poboljšanja farmakoterapije i razvoja novih lekova
SP  - 15
EP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3709
ER  - 

@conference{
author = "Trailović, Saša M. and Milovanović, Mirjana and Marjanović, Đorđe S. and Medić, Dragana and Marinković, Darko and Aničić, Milan and Stojković, Maja",
year = "2024",
abstract = "Parazitske nematode su značajni patogeni životinja i ljudi. Nažalost,
lekovi koji se koriste u farmakoterapiji helmintoza nisu apsolutno
efikasni i bezbedni protiv svih kombinacija vrsta parazita/domaćina.
Postoji jasna i hitna potreba za novim efikasnim antinematodnim jedinjenjima.
Ova potreba postaje sve važnija sa sve većim nivoom rezistencije
parazita na postojeće lekove. Većina anthelminika deluje na
neuromišićni sistem nematoda. Mi u projektu predviđamo da se dalje
ispita neuromišićni sistem na modelima parazitske nematode Ascaris
suum i slobodnoživuće nematode Caenorhabditis elegans. Fokusiraćemo
se na ispitivanje kombinaciju novih (muskarinski i serotoninski
receptori) i prethodno potvrđenih potencijalnih ciljnih mesta (jonotropni
nACh i GABA receptori). Predlažemo da se odredi lokalizacija ovih
receptora u somatskim mišićima A. suum metodom RNA-scope in situ
hibridizacije. Farmakološki ćemo okarakterisati ove receptore koristeći
kombinaciju studija pokretljivosti, testova faringijalnog pumpanja
(C. elegans), testova kontrakcije nervnomišićnog preparata (A. suum)
i elektrofiziološkim metodama “voltage“ i „current clamp“ sa dve elektrode
(A. suum). Koristeći ove tehnike, ispitivaćemo sledeće: 1. Efikasnost
kombinacija postojećih GABA-ergičnih i holinergičkih lekova u
cilju poboljšanja efikasnosti; 2. Potencijalno nove mete za anthelmintike
(muskarinski ACh receptor i serotonergički MOD 1 receptor); 3)
Prenamenu postojećih lekova koji ispoljavaju potencijalna anthelmintička
svojstva; 4) Antinematodna svojstva terpenoidnih aktivnih sastojaka
(AI) biljnih esencijalnih ulja i potencijal kombinacija AI i postojećih
lekova. Očekujemo da će naši rezultati pružiti precizniju karakterizaciju postojećih (holinergičkih i GABA-ergičnih) i potencijalnih (muskarinskih
i seretonergičkih) receptora za antiparazitske lekove. Utvrdićemo
da li neki stariji lekovi imaju potencijal da se ponovo koriste kao anthelmintici,
samostalno ili u kombinacijama.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024",
title = "Prezentacija projekta Programa Prizma 2023 Fonda za nauku Republike Srbije: Proučavanje ciljnih mesta delovanja antihelmintika u neuromuskularnom sistemu parazitskih nematoda u cilju poboljšanja farmakoterapije i razvoja novih lekova",
pages = "15-19",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3709"
}

Trailović, S. M., Milovanović, M., Marjanović, Đ. S., Medić, D., Marinković, D., Aničić, M.,& Stojković, M.. (2024). Prezentacija projekta Programa Prizma 2023 Fonda za nauku Republike Srbije: Proučavanje ciljnih mesta delovanja antihelmintika u neuromuskularnom sistemu parazitskih nematoda u cilju poboljšanja farmakoterapije i razvoja novih lekova. in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 15-19.
https://hdl.handle.net/21.15107/rcub_veterinar_3709

Trailović SM, Milovanović M, Marjanović ĐS, Medić D, Marinković D, Aničić M, Stojković M. Prezentacija projekta Programa Prizma 2023 Fonda za nauku Republike Srbije: Proučavanje ciljnih mesta delovanja antihelmintika u neuromuskularnom sistemu parazitskih nematoda u cilju poboljšanja farmakoterapije i razvoja novih lekova. in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024. 2024;:15-19.
https://hdl.handle.net/21.15107/rcub_veterinar_3709 .

Trailović, Saša M., Milovanović, Mirjana, Marjanović, Đorđe S., Medić, Dragana, Marinković, Darko, Aničić, Milan, Stojković, Maja, "Prezentacija projekta Programa Prizma 2023 Fonda za nauku Republike Srbije: Proučavanje ciljnih mesta delovanja antihelmintika u neuromuskularnom sistemu parazitskih nematoda u cilju poboljšanja farmakoterapije i razvoja novih lekova" in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024 (2024):15-19,
https://hdl.handle.net/21.15107/rcub_veterinar_3709 .

TY  - CONF
AU  - Trailović, Saša
AU  - Milovanović, Mirjana
AU  - Ivanović, Saša
AU  - Marjanović, Đorđe
AU  - Medić, Dragana
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3715
AB  - Radionica na Katedri za farmakologiju i toksikologiju će se sastojati
iz četiri dela: novine u veterinarskoj farmakoterapiji, prezentacije
usavršavanja iz farmakologije i toksikologije, propisivanje lekova
na recept i praktičnog dela primera propisivanja različitih veterinarskih
i humanih lekova na veterinarski recept.
U veterinarskoj farmakoterapiji već duže vreme nema novih lekova.
Poslednji antibiotik koji je registrovan bio je tulatromicin još 2003.
godine. Međutim, pritisak da se ograniči i smanji primena antimikrobnih
lekova je sve veći. Komitet Evropskog parlamenta za životnu sredinu,
javno zdravlje i hranu (ENVI) je 13. jula 2021. godine, podneo
predlog rezolucije Evropskom parlamentu (EP), kojom se antimikrobni
lekovi označeni od strane Svetske zdravstvene organizacije (WHO)
kao grupa visokog prioriteta i kritične važnosti za zdravlje ljudi kompletno
zabrane za upotrebu u veterinarskoj medicini. Na sreću, posle
veliko angažovanja Federacije veterinara Evrope, ova rezolucija
je odbačena.
Više godina unazad ukazuje se sve veća potreba za profilisanjem
stručnjaka iz oblasti Veterinarske farmakologiuje i toksikologije
koji će svojim znanjem, a vremenom i iskustvom moći da zadovolje
zahteve nauke, struke, privrede i državnih institucija koje se bave registracijom,
kontrolom proizvodnjom i prometom veterinarskih lekova.
Stručno usavršavanje u vidu stručne specijalizacija iz Veterinarske
farmakologije i toksikologije, predviđeno usvojenim planom i programom,
a koje će se izvoditi na Fakultetu veterinarske medicine, Univerziteta
u Beogradu namenjeno je polaznicima sa završenim Fakutetom
veterinarske medicine, farmacije i medicine.
Propisivanje lekova na recept je po definiciji pisano uputstvo
doktora veterinarske medicine ovlašćenom licu u veterinarskoj i humanoj apoteci kako da spravi i izda lek. Takođe, na receptu se objašnjava
vlasniku životinje kako da primeni lek (ukoliko za to nije potrebno
stručno znanje). Sam postupak propisivanja lekova u veterinarskoj
medicini je privilegija i obaveza isključivo doktora veterinarske medicine.
Zadatak ovog dela radionice je da osvežimo informacije o pravilima
propisivanja lekova na recepet i ukažemo na novine u propisivanju.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024
T1  - Novine u veterinarskoj farmakoterapiji, propisivanje lekova na recept i stručno usavršavanje iz farmakologije i toksikologije
SP  - 171
EP  - 177
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3715
ER  - 

@conference{
author = "Trailović, Saša and Milovanović, Mirjana and Ivanović, Saša and Marjanović, Đorđe and Medić, Dragana",
year = "2024",
abstract = "Radionica na Katedri za farmakologiju i toksikologiju će se sastojati
iz četiri dela: novine u veterinarskoj farmakoterapiji, prezentacije
usavršavanja iz farmakologije i toksikologije, propisivanje lekova
na recept i praktičnog dela primera propisivanja različitih veterinarskih
i humanih lekova na veterinarski recept.
U veterinarskoj farmakoterapiji već duže vreme nema novih lekova.
Poslednji antibiotik koji je registrovan bio je tulatromicin još 2003.
godine. Međutim, pritisak da se ograniči i smanji primena antimikrobnih
lekova je sve veći. Komitet Evropskog parlamenta za životnu sredinu,
javno zdravlje i hranu (ENVI) je 13. jula 2021. godine, podneo
predlog rezolucije Evropskom parlamentu (EP), kojom se antimikrobni
lekovi označeni od strane Svetske zdravstvene organizacije (WHO)
kao grupa visokog prioriteta i kritične važnosti za zdravlje ljudi kompletno
zabrane za upotrebu u veterinarskoj medicini. Na sreću, posle
veliko angažovanja Federacije veterinara Evrope, ova rezolucija
je odbačena.
Više godina unazad ukazuje se sve veća potreba za profilisanjem
stručnjaka iz oblasti Veterinarske farmakologiuje i toksikologije
koji će svojim znanjem, a vremenom i iskustvom moći da zadovolje
zahteve nauke, struke, privrede i državnih institucija koje se bave registracijom,
kontrolom proizvodnjom i prometom veterinarskih lekova.
Stručno usavršavanje u vidu stručne specijalizacija iz Veterinarske
farmakologije i toksikologije, predviđeno usvojenim planom i programom,
a koje će se izvoditi na Fakultetu veterinarske medicine, Univerziteta
u Beogradu namenjeno je polaznicima sa završenim Fakutetom
veterinarske medicine, farmacije i medicine.
Propisivanje lekova na recept je po definiciji pisano uputstvo
doktora veterinarske medicine ovlašćenom licu u veterinarskoj i humanoj apoteci kako da spravi i izda lek. Takođe, na receptu se objašnjava
vlasniku životinje kako da primeni lek (ukoliko za to nije potrebno
stručno znanje). Sam postupak propisivanja lekova u veterinarskoj
medicini je privilegija i obaveza isključivo doktora veterinarske medicine.
Zadatak ovog dela radionice je da osvežimo informacije o pravilima
propisivanja lekova na recepet i ukažemo na novine u propisivanju.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024",
title = "Novine u veterinarskoj farmakoterapiji, propisivanje lekova na recept i stručno usavršavanje iz farmakologije i toksikologije",
pages = "171-177",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3715"
}

Trailović, S., Milovanović, M., Ivanović, S., Marjanović, Đ.,& Medić, D.. (2024). Novine u veterinarskoj farmakoterapiji, propisivanje lekova na recept i stručno usavršavanje iz farmakologije i toksikologije. in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 171-177.
https://hdl.handle.net/21.15107/rcub_veterinar_3715

Trailović S, Milovanović M, Ivanović S, Marjanović Đ, Medić D. Novine u veterinarskoj farmakoterapiji, propisivanje lekova na recept i stručno usavršavanje iz farmakologije i toksikologije. in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024. 2024;:171-177.
https://hdl.handle.net/21.15107/rcub_veterinar_3715 .

Trailović, Saša, Milovanović, Mirjana, Ivanović, Saša, Marjanović, Đorđe, Medić, Dragana, "Novine u veterinarskoj farmakoterapiji, propisivanje lekova na recept i stručno usavršavanje iz farmakologije i toksikologije" in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024 (2024):171-177,
https://hdl.handle.net/21.15107/rcub_veterinar_3715 .

TY  - JOUR
AU  - Stefanović, Darko
AU  - Marinković, Darko
AU  - Trailović, Saša
AU  - Vasiljević, Marko
AU  - Farkaš, Hunor
AU  - Raj, Jog
AU  - Tolimir, Nataša
AU  - Radulović, Stamen
AU  - Nešić, Vladimir
AU  - Nedeljković-Trailović, Jelena
AU  - Petrujkić, Branko
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2629
AB  - This experimental study was conducted to determine the ability of a novel mycotoxins detoxification agent (MR) at a concentration of 0.2% to reduce the toxicity of aflatoxin B1 (AFB1) or T-2 toxin, alone or in combination, and to examine its effect on performance, pathohistological changes (PH) and the residue of these toxins in the tissues of broiler chicks. A total of 96 broiler chicks were divided into eight equal groups: group C, which served as control (without any additives); group MR, which received the novel detoxification agent (supplemented with 0.2%); group E-I (0.1 mg AFB1/kg of diet); group E-II (0.1 mg AFB1/kg of diet + MR 0.2%); group E-III (0.5 mg T-2 toxin/kg of diet); group E-IV (0.5 mg T-2 toxin/kg of diet + 0.2% MR); group E-V (combination of 0.1 mg AFB1/kg, 0.5 mg T-2 toxin/kg of diet); and group E-VI (combination of 0.1 mg AFB1/kg, 0.5 mg T-2 toxin + 0.2% MR). Results indicate that feeds containing AFB1 and T-2 toxin, alone or in combination, adversely affected the health and performance of poultry. However, the addition of MR to diets containing AFB1 and T-2 toxin singly and in combination exerted a positive effect on body weight, feed intake, weight gain, feed efficiency and microscopic lesions in visceral organs. Residual concentration of AFB1 in liver samples was significantly (p < 0.05) decreased when chicks were fed diets supplemented with 0.2% of MR.
PB  - MDPI
T2  - Microorganisms
T1  - Evaluation of Effectiveness of a Novel Multicomponent Mycotoxins Detoxification Agent in the Presence of AFB1 and T-2 Toxin on Broiler Chicks
VL  - 11
IS  - 3
SP  - 574
DO  - 10.3390/microorganisms11030574
ER  - 

@article{
author = "Stefanović, Darko and Marinković, Darko and Trailović, Saša and Vasiljević, Marko and Farkaš, Hunor and Raj, Jog and Tolimir, Nataša and Radulović, Stamen and Nešić, Vladimir and Nedeljković-Trailović, Jelena and Petrujkić, Branko",
year = "2023",
abstract = "This experimental study was conducted to determine the ability of a novel mycotoxins detoxification agent (MR) at a concentration of 0.2% to reduce the toxicity of aflatoxin B1 (AFB1) or T-2 toxin, alone or in combination, and to examine its effect on performance, pathohistological changes (PH) and the residue of these toxins in the tissues of broiler chicks. A total of 96 broiler chicks were divided into eight equal groups: group C, which served as control (without any additives); group MR, which received the novel detoxification agent (supplemented with 0.2%); group E-I (0.1 mg AFB1/kg of diet); group E-II (0.1 mg AFB1/kg of diet + MR 0.2%); group E-III (0.5 mg T-2 toxin/kg of diet); group E-IV (0.5 mg T-2 toxin/kg of diet + 0.2% MR); group E-V (combination of 0.1 mg AFB1/kg, 0.5 mg T-2 toxin/kg of diet); and group E-VI (combination of 0.1 mg AFB1/kg, 0.5 mg T-2 toxin + 0.2% MR). Results indicate that feeds containing AFB1 and T-2 toxin, alone or in combination, adversely affected the health and performance of poultry. However, the addition of MR to diets containing AFB1 and T-2 toxin singly and in combination exerted a positive effect on body weight, feed intake, weight gain, feed efficiency and microscopic lesions in visceral organs. Residual concentration of AFB1 in liver samples was significantly (p < 0.05) decreased when chicks were fed diets supplemented with 0.2% of MR.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Evaluation of Effectiveness of a Novel Multicomponent Mycotoxins Detoxification Agent in the Presence of AFB1 and T-2 Toxin on Broiler Chicks",
volume = "11",
number = "3",
pages = "574",
doi = "10.3390/microorganisms11030574"
}

Stefanović, D., Marinković, D., Trailović, S., Vasiljević, M., Farkaš, H., Raj, J., Tolimir, N., Radulović, S., Nešić, V., Nedeljković-Trailović, J.,& Petrujkić, B.. (2023). Evaluation of Effectiveness of a Novel Multicomponent Mycotoxins Detoxification Agent in the Presence of AFB1 and T-2 Toxin on Broiler Chicks. in Microorganisms
MDPI., 11(3), 574.
https://doi.org/10.3390/microorganisms11030574

Stefanović D, Marinković D, Trailović S, Vasiljević M, Farkaš H, Raj J, Tolimir N, Radulović S, Nešić V, Nedeljković-Trailović J, Petrujkić B. Evaluation of Effectiveness of a Novel Multicomponent Mycotoxins Detoxification Agent in the Presence of AFB1 and T-2 Toxin on Broiler Chicks. in Microorganisms. 2023;11(3):574.
doi:10.3390/microorganisms11030574 .

Stefanović, Darko, Marinković, Darko, Trailović, Saša, Vasiljević, Marko, Farkaš, Hunor, Raj, Jog, Tolimir, Nataša, Radulović, Stamen, Nešić, Vladimir, Nedeljković-Trailović, Jelena, Petrujkić, Branko, "Evaluation of Effectiveness of a Novel Multicomponent Mycotoxins Detoxification Agent in the Presence of AFB1 and T-2 Toxin on Broiler Chicks" in Microorganisms, 11, no. 3 (2023):574,
https://doi.org/10.3390/microorganisms11030574 . .

TY  - CONF
AU  - Krnjaić, Dejan
AU  - Marjanović, Đorđe
AU  - Trailović, Saša
AU  - Nešić, Vladimir
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3302
PB  - Beograd : Fakultet veterinarske medicine
C3  - 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023
T1  - Primena psihotropnih kontrolisanih supstanci i antibiotika u veterinarskoj medicini – zakonski okviri i praktični pristup
SP  - 10
EP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3302
ER  - 

@conference{
author = "Krnjaić, Dejan and Marjanović, Đorđe and Trailović, Saša and Nešić, Vladimir",
year = "2023",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023",
title = "Primena psihotropnih kontrolisanih supstanci i antibiotika u veterinarskoj medicini – zakonski okviri i praktični pristup",
pages = "10-13",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3302"
}

Krnjaić, D., Marjanović, Đ., Trailović, S.,& Nešić, V.. (2023). Primena psihotropnih kontrolisanih supstanci i antibiotika u veterinarskoj medicini – zakonski okviri i praktični pristup. in 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023
Beograd : Fakultet veterinarske medicine., 10-13.
https://hdl.handle.net/21.15107/rcub_veterinar_3302

Krnjaić D, Marjanović Đ, Trailović S, Nešić V. Primena psihotropnih kontrolisanih supstanci i antibiotika u veterinarskoj medicini – zakonski okviri i praktični pristup. in 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023. 2023;:10-13.
https://hdl.handle.net/21.15107/rcub_veterinar_3302 .

Krnjaić, Dejan, Marjanović, Đorđe, Trailović, Saša, Nešić, Vladimir, "Primena psihotropnih kontrolisanih supstanci i antibiotika u veterinarskoj medicini – zakonski okviri i praktični pristup" in 24. Regionalno savetovanje iz kliničke patologije i terapije malih životinja, Clinica veterinaria, Fruška gora, 8 - 10. jun 2023 (2023):10-13,
https://hdl.handle.net/21.15107/rcub_veterinar_3302 .

TY  - CONF
AU  - Trailović, Saša
AU  - Milovanović, Mirjana
AU  - Ivanović, Saša
AU  - Marjanović, Đorđe
AU  - Medić, Dragana
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3098
AB  - Propisivanje lekova na recept je po definiciji pisano uputstvo
doktora veterinarske medicine ovlašćenom licu u veterinarskoj i humanoj
apoteci kako da spravi i izda lek. Takođe, na receptu se objašnjava
vlasniku životinje kako da primeni lek (ukoliko za to nije potrebno
stručno znanje). Sam postupak propisivanja lekova u veterinarskoj
medicini je privilegija i obaveza isključivo doktora veterinarske medicine.
Pisanje veterinarskog recepta objedinjuje celokupno znanje iz veterinarske
farmakologije, toksikologije i kliničke prakse. Istovremeno,
pisanje recepata predstavlja i veštinu da se celokupno znanje doktora
veterinarske medicine iskoristi za dobrobit naših pacijenata. Novi propisi
i pravila u propisivanju lekova na recept zahtevaju obnovu znanja
i podsećanje na osnovna pravila propisivanja. Radionica: PROPISIVANJE
LEKOVA NA RECEPT, obuhvatiće: 1. Pravilnike o propisivanju
leka na recept (veterinarski i humani), elektronski recepti u humanoj
praksi, najavu elektronskih recepata u veterinarskoj medicini u EU,
pravilo kaskada i ulazni test; 2. Propisivanje veterinarskih lekova na
recept, osnovna pravila i primere; 3. Propisivanje humanih lekova na
veterinarski recept sa posebnim osvrtom na propisivanje psihotropnih
kontrolisanih supstanci; 4. Primere propisivanja lekova, veterinarskih i
humanih, vežbanje propisivanja lekova, Izlazni test.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023
T1  - Propisivanje lekova na recept
SP  - 125
EP  - 128
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3098
ER  - 

@conference{
author = "Trailović, Saša and Milovanović, Mirjana and Ivanović, Saša and Marjanović, Đorđe and Medić, Dragana",
year = "2023",
abstract = "Propisivanje lekova na recept je po definiciji pisano uputstvo
doktora veterinarske medicine ovlašćenom licu u veterinarskoj i humanoj
apoteci kako da spravi i izda lek. Takođe, na receptu se objašnjava
vlasniku životinje kako da primeni lek (ukoliko za to nije potrebno
stručno znanje). Sam postupak propisivanja lekova u veterinarskoj
medicini je privilegija i obaveza isključivo doktora veterinarske medicine.
Pisanje veterinarskog recepta objedinjuje celokupno znanje iz veterinarske
farmakologije, toksikologije i kliničke prakse. Istovremeno,
pisanje recepata predstavlja i veštinu da se celokupno znanje doktora
veterinarske medicine iskoristi za dobrobit naših pacijenata. Novi propisi
i pravila u propisivanju lekova na recept zahtevaju obnovu znanja
i podsećanje na osnovna pravila propisivanja. Radionica: PROPISIVANJE
LEKOVA NA RECEPT, obuhvatiće: 1. Pravilnike o propisivanju
leka na recept (veterinarski i humani), elektronski recepti u humanoj
praksi, najavu elektronskih recepata u veterinarskoj medicini u EU,
pravilo kaskada i ulazni test; 2. Propisivanje veterinarskih lekova na
recept, osnovna pravila i primere; 3. Propisivanje humanih lekova na
veterinarski recept sa posebnim osvrtom na propisivanje psihotropnih
kontrolisanih supstanci; 4. Primere propisivanja lekova, veterinarskih i
humanih, vežbanje propisivanja lekova, Izlazni test.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023",
title = "Propisivanje lekova na recept",
pages = "125-128",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3098"
}

Trailović, S., Milovanović, M., Ivanović, S., Marjanović, Đ.,& Medić, D.. (2023). Propisivanje lekova na recept. in XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 125-128.
https://hdl.handle.net/21.15107/rcub_veterinar_3098

Trailović S, Milovanović M, Ivanović S, Marjanović Đ, Medić D. Propisivanje lekova na recept. in XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023. 2023;:125-128.
https://hdl.handle.net/21.15107/rcub_veterinar_3098 .

Trailović, Saša, Milovanović, Mirjana, Ivanović, Saša, Marjanović, Đorđe, Medić, Dragana, "Propisivanje lekova na recept" in XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023 (2023):125-128,
https://hdl.handle.net/21.15107/rcub_veterinar_3098 .

TY  - CONF
AU  - Milovanović, Mirjana
AU  - Marjanović, Đorđe
AU  - Trailović, Saša
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3253
AB  - Bol je jedan od najstarijih senzornih modaliteta prisutan kod sisara i filogenetski najnižih životinjskih vrsta. Prateći je simptom povreda i bolesti, sa svrhom da upozori organizam na odbranu od štetnog nadražaja. Međutim, ukoliko se bolni nadražaj ne može ukloniti, tada bolno stanje poprima hroničan tok i postaje bolest sama za sebe. Farmakoterapija bola se zasniva, pre svega, na primeni opioidnih analgetika, analgo-antipiretika i nesteroidnih antiinflamatornih lekova (NSAIL), sa potvrđenim analgetičkim dejstvom ali i umerenim do izraženim neželjenim efektima. S toga je razumljiva potreba za pronalaženje novih, efikasnih i bezbednih analgetika za uvođenje u kliničku praksu. 
Polazeći od ovog stanovništva, naše interesovanje usmerili smo ka ispitivanju analgetičke aktivnosti i mehanizme delovanja monoterpena karvakrola i p-cimena, aktivnih sastojaka esencijalnih biljnih ulja iz origana, majčine dušice, šargarepe, kao i fenilpropanoida cinamaldehida iz isparljivih ulja cimeta, na inflamatorni bol kod pacova. 
Za potrebe ovog istraživanja koristili smo dva različita testa provere analgetičke aktivnosti: orofacijalni formalni test za karvakrol i test mehaničkog pritiska šape pacova inflamirane karageninom meren von-Frey aesteziometrom za p-cimen i cinamaldehid. 
U ovom istraživanju karvakrol i p-cimen ispoljavaju dobro izraženu dozno-zavisnu analgetičku aktivnost, dok cinamaldehid ima slabije izražen analgetički efekat na inflamatorni bol. Takođe, primenom istih farmakoloških testova pokazano je da u mehanizmu analgetičkog delovanja navedenih aktivnih sastojaka značajno mesto zauzima L-arginin-NO-sistem. Monoterpeni karvakrol i p-cimen ne narušavaju motornu koordinaciju pacova ispitanu rota-rod testom, čime se isključuje njihovo neželjeno dejstvo na motornu funkciju CNS-a. 
Analizam dobijenih rezultat da se zaključiti da ispitani aktivni sastojci esencijalnih biljnih ulja, a pre svega karvakrol i p-cimen, predstavljaju ozbiljne kandidate za dalja pretklinička i klinička istraživanja kao mogući analgetici, koji bi bili dovoljno efikasni i bezbedni za primenu u humanoj i vetreinarskoj medicini.
PB  - Beograd : Klinički centar Srbije - Klinika za psihijatriju i Udruženje psihijatara Srbije
C3  - Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023
T1  - Analgetsko delovanje monoterpena i fenilpropanoida iz esencijalnizh biljnih ulja - mogućnost primene u veterinarskoj i humanoj kliničkoj praksi
VL  - 45
IS  - 1
SP  - 86
EP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3253
ER  - 

@conference{
author = "Milovanović, Mirjana and Marjanović, Đorđe and Trailović, Saša",
year = "2023",
abstract = "Bol je jedan od najstarijih senzornih modaliteta prisutan kod sisara i filogenetski najnižih životinjskih vrsta. Prateći je simptom povreda i bolesti, sa svrhom da upozori organizam na odbranu od štetnog nadražaja. Međutim, ukoliko se bolni nadražaj ne može ukloniti, tada bolno stanje poprima hroničan tok i postaje bolest sama za sebe. Farmakoterapija bola se zasniva, pre svega, na primeni opioidnih analgetika, analgo-antipiretika i nesteroidnih antiinflamatornih lekova (NSAIL), sa potvrđenim analgetičkim dejstvom ali i umerenim do izraženim neželjenim efektima. S toga je razumljiva potreba za pronalaženje novih, efikasnih i bezbednih analgetika za uvođenje u kliničku praksu. 
Polazeći od ovog stanovništva, naše interesovanje usmerili smo ka ispitivanju analgetičke aktivnosti i mehanizme delovanja monoterpena karvakrola i p-cimena, aktivnih sastojaka esencijalnih biljnih ulja iz origana, majčine dušice, šargarepe, kao i fenilpropanoida cinamaldehida iz isparljivih ulja cimeta, na inflamatorni bol kod pacova. 
Za potrebe ovog istraživanja koristili smo dva različita testa provere analgetičke aktivnosti: orofacijalni formalni test za karvakrol i test mehaničkog pritiska šape pacova inflamirane karageninom meren von-Frey aesteziometrom za p-cimen i cinamaldehid. 
U ovom istraživanju karvakrol i p-cimen ispoljavaju dobro izraženu dozno-zavisnu analgetičku aktivnost, dok cinamaldehid ima slabije izražen analgetički efekat na inflamatorni bol. Takođe, primenom istih farmakoloških testova pokazano je da u mehanizmu analgetičkog delovanja navedenih aktivnih sastojaka značajno mesto zauzima L-arginin-NO-sistem. Monoterpeni karvakrol i p-cimen ne narušavaju motornu koordinaciju pacova ispitanu rota-rod testom, čime se isključuje njihovo neželjeno dejstvo na motornu funkciju CNS-a. 
Analizam dobijenih rezultat da se zaključiti da ispitani aktivni sastojci esencijalnih biljnih ulja, a pre svega karvakrol i p-cimen, predstavljaju ozbiljne kandidate za dalja pretklinička i klinička istraživanja kao mogući analgetici, koji bi bili dovoljno efikasni i bezbedni za primenu u humanoj i vetreinarskoj medicini.",
publisher = "Beograd : Klinički centar Srbije - Klinika za psihijatriju i Udruženje psihijatara Srbije",
journal = "Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023",
title = "Analgetsko delovanje monoterpena i fenilpropanoida iz esencijalnizh biljnih ulja - mogućnost primene u veterinarskoj i humanoj kliničkoj praksi",
volume = "45",
number = "1",
pages = "86-87",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3253"
}

Milovanović, M., Marjanović, Đ.,& Trailović, S.. (2023). Analgetsko delovanje monoterpena i fenilpropanoida iz esencijalnizh biljnih ulja - mogućnost primene u veterinarskoj i humanoj kliničkoj praksi. in Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023
Beograd : Klinički centar Srbije - Klinika za psihijatriju i Udruženje psihijatara Srbije., 45(1), 86-87.
https://hdl.handle.net/21.15107/rcub_veterinar_3253

Milovanović M, Marjanović Đ, Trailović S. Analgetsko delovanje monoterpena i fenilpropanoida iz esencijalnizh biljnih ulja - mogućnost primene u veterinarskoj i humanoj kliničkoj praksi. in Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023. 2023;45(1):86-87.
https://hdl.handle.net/21.15107/rcub_veterinar_3253 .

Milovanović, Mirjana, Marjanović, Đorđe, Trailović, Saša, "Analgetsko delovanje monoterpena i fenilpropanoida iz esencijalnizh biljnih ulja - mogućnost primene u veterinarskoj i humanoj kliničkoj praksi" in Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023, 45, no. 1 (2023):86-87,
https://hdl.handle.net/21.15107/rcub_veterinar_3253 .

TY  - CONF
AU  - Ivanović, Saša R.
AU  - Trailović, Saša M.
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3265
PB  - Beograd : Klinički centar Srbije - Klinika za psihijatriju i Udruženje psihijatara Srbije
C3  - Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023
T1  - The GABA receptor of parasitic nematodes as a target site for antinematodal drugs
VL  - 45
IS  - 1
SP  - 42
EP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3265
ER  - 

@conference{
author = "Ivanović, Saša R. and Trailović, Saša M.",
year = "2023",
publisher = "Beograd : Klinički centar Srbije - Klinika za psihijatriju i Udruženje psihijatara Srbije",
journal = "Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023",
title = "The GABA receptor of parasitic nematodes as a target site for antinematodal drugs",
volume = "45",
number = "1",
pages = "42-43",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3265"
}

Ivanović, S. R.,& Trailović, S. M.. (2023). The GABA receptor of parasitic nematodes as a target site for antinematodal drugs. in Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023
Beograd : Klinički centar Srbije - Klinika za psihijatriju i Udruženje psihijatara Srbije., 45(1), 42-43.
https://hdl.handle.net/21.15107/rcub_veterinar_3265

Ivanović SR, Trailović SM. The GABA receptor of parasitic nematodes as a target site for antinematodal drugs. in Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023. 2023;45(1):42-43.
https://hdl.handle.net/21.15107/rcub_veterinar_3265 .

Ivanović, Saša R., Trailović, Saša M., "The GABA receptor of parasitic nematodes as a target site for antinematodal drugs" in Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023, 45, no. 1 (2023):42-43,
https://hdl.handle.net/21.15107/rcub_veterinar_3265 .

TY  - CONF
AU  - Marjanović, Đorđe
AU  - Trailović, Saša
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3296
AB  - Prema podacima iz literature bolesti prouzrokovane parazitskim nematodama pogađaju veliki broj stanovnika na svetu (SZO-1.6 milijardi za 2022.), a vrlo često mogu biti i uzrok smrti u neadekvatnim higijensko-sanitarnim uslovima življenja. Ono što je zajedničko za parazitske infekcije životinja i ljudi, odnosno parazite koji ih napadaju jeste sposobnost da vrlo brzo nakon primene antiparazitskih lekova razvijaju rezistenciju na primenu istih. Pokazalo se na osnovu naših dosadašnjih istraživanja da je velika svinjska glista (A. suum) odličan alternativni model za ispitivanja antinematodnih aktivnosti postojećih i potencijalno novih antiparazitskih lekova.U ovom radu provereni su i analizirani mehanizmi kojima pojedini aktivni sastojci esencijalnih biljnih ulja deluju na neuromišićni preparat  parazitske nematode A. suum koja je veoma čest parazit u crevima svinja. Ispitan je mehanizam antinematodnog dejstva odabranih biljnih mono i diterpenoidа na neuromišićnom preparatu nematode i dejstvo na različite podtipove nikotinskog receptora (L, N i B tip. Pored toga ispitan je uticaj i interakcija terpenoidnih aktivnih sastojaka biljnih ulja na GABA receptor parazitske nematode. Na osnovu naših rezultata (Marjanović i sar., 2020) inhibitorno dejstvo karvakrola na kontrakcije neuromišićnog preparata A. suum odigrava se posredstvom nikotinskog acetilholinskog receptora (nAChR). Inhibitorno dejstvo karvakrola ispoljava karakteristike kompetitivnog i nekompetitivnog antagonizma. Za istraživanja antinematodnog dejstva potencijalno novih lekova u našoj laboratoriji je prvi put korišćen u ovom delu Evrope i slobodno živeća nematoda  Caenorhabditis elegans. U radu je prikazan uticaj timola i karvakrola na preživljavanje i faringealno pumpanje nematode Caenorhabditis elegans kao i dozna i vremenska zavisnost ovog efekta.
PB  - Beograd : Klinički centar Srbije - Klinika za psihijatriju i Udruženje psihijatara Srbije
C3  - Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023
T1  - Antinematodna dejstva odabranih mono i diterpenoidnih aktivnih sastojaka esencijalnih biljnih ulja
VL  - 45
IS  - 1
SP  - 64
EP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3296
ER  - 

@conference{
author = "Marjanović, Đorđe and Trailović, Saša",
year = "2023",
abstract = "Prema podacima iz literature bolesti prouzrokovane parazitskim nematodama pogađaju veliki broj stanovnika na svetu (SZO-1.6 milijardi za 2022.), a vrlo često mogu biti i uzrok smrti u neadekvatnim higijensko-sanitarnim uslovima življenja. Ono što je zajedničko za parazitske infekcije životinja i ljudi, odnosno parazite koji ih napadaju jeste sposobnost da vrlo brzo nakon primene antiparazitskih lekova razvijaju rezistenciju na primenu istih. Pokazalo se na osnovu naših dosadašnjih istraživanja da je velika svinjska glista (A. suum) odličan alternativni model za ispitivanja antinematodnih aktivnosti postojećih i potencijalno novih antiparazitskih lekova.U ovom radu provereni su i analizirani mehanizmi kojima pojedini aktivni sastojci esencijalnih biljnih ulja deluju na neuromišićni preparat  parazitske nematode A. suum koja je veoma čest parazit u crevima svinja. Ispitan je mehanizam antinematodnog dejstva odabranih biljnih mono i diterpenoidа na neuromišićnom preparatu nematode i dejstvo na različite podtipove nikotinskog receptora (L, N i B tip. Pored toga ispitan je uticaj i interakcija terpenoidnih aktivnih sastojaka biljnih ulja na GABA receptor parazitske nematode. Na osnovu naših rezultata (Marjanović i sar., 2020) inhibitorno dejstvo karvakrola na kontrakcije neuromišićnog preparata A. suum odigrava se posredstvom nikotinskog acetilholinskog receptora (nAChR). Inhibitorno dejstvo karvakrola ispoljava karakteristike kompetitivnog i nekompetitivnog antagonizma. Za istraživanja antinematodnog dejstva potencijalno novih lekova u našoj laboratoriji je prvi put korišćen u ovom delu Evrope i slobodno živeća nematoda  Caenorhabditis elegans. U radu je prikazan uticaj timola i karvakrola na preživljavanje i faringealno pumpanje nematode Caenorhabditis elegans kao i dozna i vremenska zavisnost ovog efekta.",
publisher = "Beograd : Klinički centar Srbije - Klinika za psihijatriju i Udruženje psihijatara Srbije",
journal = "Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023",
title = "Antinematodna dejstva odabranih mono i diterpenoidnih aktivnih sastojaka esencijalnih biljnih ulja",
volume = "45",
number = "1",
pages = "64-64",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3296"
}

Marjanović, Đ.,& Trailović, S.. (2023). Antinematodna dejstva odabranih mono i diterpenoidnih aktivnih sastojaka esencijalnih biljnih ulja. in Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023
Beograd : Klinički centar Srbije - Klinika za psihijatriju i Udruženje psihijatara Srbije., 45(1), 64-64.
https://hdl.handle.net/21.15107/rcub_veterinar_3296

Marjanović Đ, Trailović S. Antinematodna dejstva odabranih mono i diterpenoidnih aktivnih sastojaka esencijalnih biljnih ulja. in Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023. 2023;45(1):64-64.
https://hdl.handle.net/21.15107/rcub_veterinar_3296 .

Marjanović, Đorđe, Trailović, Saša, "Antinematodna dejstva odabranih mono i diterpenoidnih aktivnih sastojaka esencijalnih biljnih ulja" in Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023, 45, no. 1 (2023):64-64,
https://hdl.handle.net/21.15107/rcub_veterinar_3296 .

TY  - JOUR
AU  - Vasiljević, Marko
AU  - Milićević, Dragan
AU  - Pleadin, Jelka
AU  - Tolmir, Nataša
AU  - Trailović, Saša
AU  - Resanović, Radmila
AU  - Nedeljković-Trailović, Jelena
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2627
AB  - The objective of this study was to evaluate the efficacy of modified clinoptilolite as mycotoxins adsorbent in preventing negative effects of ochratoxin A (OTA) on egg production and egg quality of laying hens exposed to this mycotoxin. Forty-eight (n=48) laying hens (27 weeks old) were used in this study. The hens were randomly divided into six equal groups and were fed for 7 weeks with a standard diet in addition to: E-I group - 1 mg/kg OTA; E-II group 0.25 mg/kg OTA; E-III group 1mg/kg OTA + 0.2% of MZ; E-IV group 0.25 mg/kg OTA + 0.2% of MZ. MZ group of hens was fed with standard diets containing 0.2% of the adsorbent (MZ). The control group of hens was fed with standard diet, without any addition of OTA or MZ. The present study showed that laying hens fed with 1 mg/kg of OTA (E-I), had a significant decrease (p<0.05) of all performance parameters during the trial, while group fed with 0.25 mg/kg OTA have shown no adverse effects on egg production and egg quality. Addition of modified clinoptilolite (0.2%) to the diet containing the OTA, minimized these effects bringing values not significantly different from the control diet for most of the parameters. These findings clearly indicate the protective potential of modified clinoptilolite against the toxic effects of OTA in laying hens.
PB  - Foundation of Poultry Science and Technology - FACTA.
T2  - Brazilian Journal of Poultry Science
T1  - Effect of Modified Clinoptilolite to Counteract the Deleterious Effects of Ochratoxin A on Egg Production and Quality
VL  - 24
IS  - 3
DO  - 10.1590/1806-9061-2021-1495
ER  - 

@article{
author = "Vasiljević, Marko and Milićević, Dragan and Pleadin, Jelka and Tolmir, Nataša and Trailović, Saša and Resanović, Radmila and Nedeljković-Trailović, Jelena",
year = "2022",
abstract = "The objective of this study was to evaluate the efficacy of modified clinoptilolite as mycotoxins adsorbent in preventing negative effects of ochratoxin A (OTA) on egg production and egg quality of laying hens exposed to this mycotoxin. Forty-eight (n=48) laying hens (27 weeks old) were used in this study. The hens were randomly divided into six equal groups and were fed for 7 weeks with a standard diet in addition to: E-I group - 1 mg/kg OTA; E-II group 0.25 mg/kg OTA; E-III group 1mg/kg OTA + 0.2% of MZ; E-IV group 0.25 mg/kg OTA + 0.2% of MZ. MZ group of hens was fed with standard diets containing 0.2% of the adsorbent (MZ). The control group of hens was fed with standard diet, without any addition of OTA or MZ. The present study showed that laying hens fed with 1 mg/kg of OTA (E-I), had a significant decrease (p<0.05) of all performance parameters during the trial, while group fed with 0.25 mg/kg OTA have shown no adverse effects on egg production and egg quality. Addition of modified clinoptilolite (0.2%) to the diet containing the OTA, minimized these effects bringing values not significantly different from the control diet for most of the parameters. These findings clearly indicate the protective potential of modified clinoptilolite against the toxic effects of OTA in laying hens.",
publisher = "Foundation of Poultry Science and Technology - FACTA.",
journal = "Brazilian Journal of Poultry Science",
title = "Effect of Modified Clinoptilolite to Counteract the Deleterious Effects of Ochratoxin A on Egg Production and Quality",
volume = "24",
number = "3",
doi = "10.1590/1806-9061-2021-1495"
}

Vasiljević, M., Milićević, D., Pleadin, J., Tolmir, N., Trailović, S., Resanović, R.,& Nedeljković-Trailović, J.. (2022). Effect of Modified Clinoptilolite to Counteract the Deleterious Effects of Ochratoxin A on Egg Production and Quality. in Brazilian Journal of Poultry Science
Foundation of Poultry Science and Technology - FACTA.., 24(3).
https://doi.org/10.1590/1806-9061-2021-1495

Vasiljević M, Milićević D, Pleadin J, Tolmir N, Trailović S, Resanović R, Nedeljković-Trailović J. Effect of Modified Clinoptilolite to Counteract the Deleterious Effects of Ochratoxin A on Egg Production and Quality. in Brazilian Journal of Poultry Science. 2022;24(3).
doi:10.1590/1806-9061-2021-1495 .

Vasiljević, Marko, Milićević, Dragan, Pleadin, Jelka, Tolmir, Nataša, Trailović, Saša, Resanović, Radmila, Nedeljković-Trailović, Jelena, "Effect of Modified Clinoptilolite to Counteract the Deleterious Effects of Ochratoxin A on Egg Production and Quality" in Brazilian Journal of Poultry Science, 24, no. 3 (2022),
https://doi.org/10.1590/1806-9061-2021-1495 . .

TY  - JOUR
AU  - Pešić, Branislav
AU  - Kulišić, Zoran
AU  - Teodorović, Radislava
AU  - Trailović, Saša M.
AU  - Djokić, Vitomir
AU  - Djordjevic, Milutin
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2459
AB  - Culex mosquitos are important infectious diseases vectors in temperate and tropical regions of the World, affecting nearly 350 million people in both developed and developing countries. Our approach was to “recycle” the well-established larvicide, and by studying the tablets, pellets and granules as floating or sinking formulations, we found a method to optimise the use of diflubenzuron against Culex pipiens mosquitoes in field conditions.A standard WHO procedure was used to test the larvicide efficacy. The combined efficacy of all floating formulations was 10.7% higher than sinking preparations (p-value =0.002) and that maximised throughout the experiment on days 14 and 21, (p-values 0.012 and 0.008, respectively). All floating formulations kept their efficacies above 70% until day 21 of the experiment, while the mortality of sinking designs dropped significantly after day 14. The lowest efficacy was observed when sinking granules were used and the highest when floating tablets were applied in the canals. Only the floating tablets showed no significant changes in efficacy from day 1 to 21, but then that efficacy drops sharp until day 35.Since the larvae spend most of their time on the surface of the slow-moving waters to breathe, and floating pellets and tablets are made of materials that  can  serve  as  food  sources,  we  conclude  that  these  formulations  have  a  higher  effi  cacy,  at  least  in  conditions  of   non-  or  slow-moving  waters.  This  study  shows  the  importance  of   a  systematic approach to reformulation of  old, already proven and environmentally safe larvicides which can control the mosquito populations and their spreading of various pathogens.
PB  - Faculty of Veterinary Medicine, University of Belgrade
T2  - Acta Veterinaria
T1  - Comparison of mosquito larvicidal formulations of diflubenzuron on Culex pipiens mosquitoes in Belgrade, Serbia
T1  - Poređenje formulacija larvicida na bazi diflubenzurona na komarcima culex pipiens u beogradu, srbija
VL  - 72
IS  - 1
SP  - 87
EP  - 99
DO  - 10.2478/acve-2022-0007
ER  - 

@article{
author = "Pešić, Branislav and Kulišić, Zoran and Teodorović, Radislava and Trailović, Saša M. and Djokić, Vitomir and Djordjevic, Milutin",
year = "2022",
abstract = "Culex mosquitos are important infectious diseases vectors in temperate and tropical regions of the World, affecting nearly 350 million people in both developed and developing countries. Our approach was to “recycle” the well-established larvicide, and by studying the tablets, pellets and granules as floating or sinking formulations, we found a method to optimise the use of diflubenzuron against Culex pipiens mosquitoes in field conditions.A standard WHO procedure was used to test the larvicide efficacy. The combined efficacy of all floating formulations was 10.7% higher than sinking preparations (p-value =0.002) and that maximised throughout the experiment on days 14 and 21, (p-values 0.012 and 0.008, respectively). All floating formulations kept their efficacies above 70% until day 21 of the experiment, while the mortality of sinking designs dropped significantly after day 14. The lowest efficacy was observed when sinking granules were used and the highest when floating tablets were applied in the canals. Only the floating tablets showed no significant changes in efficacy from day 1 to 21, but then that efficacy drops sharp until day 35.Since the larvae spend most of their time on the surface of the slow-moving waters to breathe, and floating pellets and tablets are made of materials that  can  serve  as  food  sources,  we  conclude  that  these  formulations  have  a  higher  effi  cacy,  at  least  in  conditions  of   non-  or  slow-moving  waters.  This  study  shows  the  importance  of   a  systematic approach to reformulation of  old, already proven and environmentally safe larvicides which can control the mosquito populations and their spreading of various pathogens.",
publisher = "Faculty of Veterinary Medicine, University of Belgrade",
journal = "Acta Veterinaria",
title = "Comparison of mosquito larvicidal formulations of diflubenzuron on Culex pipiens mosquitoes in Belgrade, Serbia, Poređenje formulacija larvicida na bazi diflubenzurona na komarcima culex pipiens u beogradu, srbija",
volume = "72",
number = "1",
pages = "87-99",
doi = "10.2478/acve-2022-0007"
}

Pešić, B., Kulišić, Z., Teodorović, R., Trailović, S. M., Djokić, V.,& Djordjevic, M.. (2022). Comparison of mosquito larvicidal formulations of diflubenzuron on Culex pipiens mosquitoes in Belgrade, Serbia. in Acta Veterinaria
Faculty of Veterinary Medicine, University of Belgrade., 72(1), 87-99.
https://doi.org/10.2478/acve-2022-0007

Pešić B, Kulišić Z, Teodorović R, Trailović SM, Djokić V, Djordjevic M. Comparison of mosquito larvicidal formulations of diflubenzuron on Culex pipiens mosquitoes in Belgrade, Serbia. in Acta Veterinaria. 2022;72(1):87-99.
doi:10.2478/acve-2022-0007 .

Pešić, Branislav, Kulišić, Zoran, Teodorović, Radislava, Trailović, Saša M., Djokić, Vitomir, Djordjevic, Milutin, "Comparison of mosquito larvicidal formulations of diflubenzuron on Culex pipiens mosquitoes in Belgrade, Serbia" in Acta Veterinaria, 72, no. 1 (2022):87-99,
https://doi.org/10.2478/acve-2022-0007 . .

TY  - JOUR
AU  - Đukanović, Đorđe
AU  - Gajić Bojić, Milica
AU  - Marinković, Sonja
AU  - Trailović, Saša
AU  - Stojiljkovic, Miloš P.
AU  - Škrbić, Ranko
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2628
AB  - Carvacrol (CRV) is the main compound of essential oils extracted primarily from Thymus and Origanum species. Its various biological activities were confirmed: antioxidant, anti-inflammatory, antibacterial, antifungal, anti-tumour, antinematodal, and vasorelaxant action. Although vasodilation mediated by CRV was previously described, the exact mechanism of its action has not yet been established. Hence, the aim of this study was to investigate CRV vasoactivity on human umbilical arteries (HUA) and the different pathways involved in its mechanism of action using the tissue bath methodology. CRV caused a significant decrease in vascular tension of 5-HT-pre-contracted umbilical arteries, with EC50 of 442.13 ± 33.8 µmol/L (mean ± standard error of the mean—SEM). At 300 µmol/L, CRV shifted downward the 5-HT concentration–response curve with a statistical significance of p < 0.001 obtained for the four highest concentrations. At a concentration of 1 mmol/L, CRV completely abolished BaCl2-induced contraction in Ca2+-free Krebs–Ringer bicarbonate solution and the BAY K 8644-induced contraction in Krebs–Ringer bicarbonate solution (p < 0.001). Isopentenyl pyrophosphate, the antagonist of TRPV3 channel, was able to decrease the efficacy of CRV (p < 0.001). The blocking of L-type Ca2+ channels on smooth muscle cells is the most probable mechanism of CRV-induced vasorelaxation. However, the role of TRPV3 channels in CRV-induced vasodilation of HUA cannot be excluded either.
PB  - Canadian Science Publishing
T2  - Canadian Journal of Physiology and Pharmacology
T1  - Vasorelaxant effect of monoterpene carvacrol on isolated human umbilical artery
VL  - 100
SP  - 755
SP  - 762
DO  - 10.1139/cjpp-2021-0736
ER  - 

@article{
author = "Đukanović, Đorđe and Gajić Bojić, Milica and Marinković, Sonja and Trailović, Saša and Stojiljkovic, Miloš P. and Škrbić, Ranko",
year = "2022",
abstract = "Carvacrol (CRV) is the main compound of essential oils extracted primarily from Thymus and Origanum species. Its various biological activities were confirmed: antioxidant, anti-inflammatory, antibacterial, antifungal, anti-tumour, antinematodal, and vasorelaxant action. Although vasodilation mediated by CRV was previously described, the exact mechanism of its action has not yet been established. Hence, the aim of this study was to investigate CRV vasoactivity on human umbilical arteries (HUA) and the different pathways involved in its mechanism of action using the tissue bath methodology. CRV caused a significant decrease in vascular tension of 5-HT-pre-contracted umbilical arteries, with EC50 of 442.13 ± 33.8 µmol/L (mean ± standard error of the mean—SEM). At 300 µmol/L, CRV shifted downward the 5-HT concentration–response curve with a statistical significance of p < 0.001 obtained for the four highest concentrations. At a concentration of 1 mmol/L, CRV completely abolished BaCl2-induced contraction in Ca2+-free Krebs–Ringer bicarbonate solution and the BAY K 8644-induced contraction in Krebs–Ringer bicarbonate solution (p < 0.001). Isopentenyl pyrophosphate, the antagonist of TRPV3 channel, was able to decrease the efficacy of CRV (p < 0.001). The blocking of L-type Ca2+ channels on smooth muscle cells is the most probable mechanism of CRV-induced vasorelaxation. However, the role of TRPV3 channels in CRV-induced vasodilation of HUA cannot be excluded either.",
publisher = "Canadian Science Publishing",
journal = "Canadian Journal of Physiology and Pharmacology",
title = "Vasorelaxant effect of monoterpene carvacrol on isolated human umbilical artery",
volume = "100",
pages = "755-762",
doi = "10.1139/cjpp-2021-0736"
}

Đukanović, Đ., Gajić Bojić, M., Marinković, S., Trailović, S., Stojiljkovic, M. P.,& Škrbić, R.. (2022). Vasorelaxant effect of monoterpene carvacrol on isolated human umbilical artery. in Canadian Journal of Physiology and Pharmacology
Canadian Science Publishing., 100, 755.
https://doi.org/10.1139/cjpp-2021-0736

Đukanović Đ, Gajić Bojić M, Marinković S, Trailović S, Stojiljkovic MP, Škrbić R. Vasorelaxant effect of monoterpene carvacrol on isolated human umbilical artery. in Canadian Journal of Physiology and Pharmacology. 2022;100:755.
doi:10.1139/cjpp-2021-0736 .

Đukanović, Đorđe, Gajić Bojić, Milica, Marinković, Sonja, Trailović, Saša, Stojiljkovic, Miloš P., Škrbić, Ranko, "Vasorelaxant effect of monoterpene carvacrol on isolated human umbilical artery" in Canadian Journal of Physiology and Pharmacology, 100 (2022):755,
https://doi.org/10.1139/cjpp-2021-0736 . .

TY  - CONF
AU  - Trailović, Saša M.
AU  - Marinković, Darko
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3343
AB  - U veterinarskoj medicini postoji potreba za individualnom terapijom i terapijom
stada/krda/jata, kada se istovremeno tretira veći broj životinja. Sprovođenje individualne terapije uglavnom ne predstavlja veći problem, ali istovremena primena lekova
kod velikog broja životinja zahteva posebnu opreznost, precizno doziranje i potrebu
da životinje dobiju adekvatnu dozu leka. Ovo je posebno teško sprovesti kada postoji
potreba za tretiranjem krda divljih životinja. Naša iskustva se odnose na primenu antiparazitskih lekova kod jelenske divljači (Cervus elaphus i Dama dama) u ograđenom
i otvorenom lovištu i divljih svinja (Sus scrofa) u gaterima. U tretmanu smo koristili
benzimidazole, triklabendazol i albendazol kod jelena i ivermektin kod divljih svinja.
Lekove smo primenjivali nanete na kukuruz, u blisterima soli, odnosno umešane u prekrupu (ivermektin). Osnovni problemi na koje smo nailazili su bili: smanjena palatibilnost hrane na koju je nanešen lek, problem kontrole količine leka koju su životinje unosile (kontrola doziranja), osetljivost lekova na tehnološke procese u blistiranju soli kao
i promene na samim solištima koje smo morali da obavimo da bi obezbedili postojanost
leka. Naša iskustva ukazuju da je potrebno najmanje jedna do dve nedelje navikavanja
životinja na nove uslove prihrane da bi se posle tog perioda mogla odrediti doza leka i
dužina tretmana. U ovom radu detaljno su prikazane tehnike davanja antiparazitskih
lekova divljim papkarima hranom i efikasnost ovakve terapije.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 33. Savetovanje veterinara Srbije, Zlatibor, 8 - 11. septembar 2022
T1  - Farmakoterapija parazitskih infekcija divljači, naša iskustva
SP  - 168
EP  - 173
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3343
ER  - 

@conference{
author = "Trailović, Saša M. and Marinković, Darko",
year = "2022",
abstract = "U veterinarskoj medicini postoji potreba za individualnom terapijom i terapijom
stada/krda/jata, kada se istovremeno tretira veći broj životinja. Sprovođenje individualne terapije uglavnom ne predstavlja veći problem, ali istovremena primena lekova
kod velikog broja životinja zahteva posebnu opreznost, precizno doziranje i potrebu
da životinje dobiju adekvatnu dozu leka. Ovo je posebno teško sprovesti kada postoji
potreba za tretiranjem krda divljih životinja. Naša iskustva se odnose na primenu antiparazitskih lekova kod jelenske divljači (Cervus elaphus i Dama dama) u ograđenom
i otvorenom lovištu i divljih svinja (Sus scrofa) u gaterima. U tretmanu smo koristili
benzimidazole, triklabendazol i albendazol kod jelena i ivermektin kod divljih svinja.
Lekove smo primenjivali nanete na kukuruz, u blisterima soli, odnosno umešane u prekrupu (ivermektin). Osnovni problemi na koje smo nailazili su bili: smanjena palatibilnost hrane na koju je nanešen lek, problem kontrole količine leka koju su životinje unosile (kontrola doziranja), osetljivost lekova na tehnološke procese u blistiranju soli kao
i promene na samim solištima koje smo morali da obavimo da bi obezbedili postojanost
leka. Naša iskustva ukazuju da je potrebno najmanje jedna do dve nedelje navikavanja
životinja na nove uslove prihrane da bi se posle tog perioda mogla odrediti doza leka i
dužina tretmana. U ovom radu detaljno su prikazane tehnike davanja antiparazitskih
lekova divljim papkarima hranom i efikasnost ovakve terapije.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "33. Savetovanje veterinara Srbije, Zlatibor, 8 - 11. septembar 2022",
title = "Farmakoterapija parazitskih infekcija divljači, naša iskustva",
pages = "168-173",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3343"
}

Trailović, S. M.,& Marinković, D.. (2022). Farmakoterapija parazitskih infekcija divljači, naša iskustva. in 33. Savetovanje veterinara Srbije, Zlatibor, 8 - 11. septembar 2022
Beograd : Srpsko veterinarsko društvo., 168-173.
https://hdl.handle.net/21.15107/rcub_veterinar_3343

Trailović SM, Marinković D. Farmakoterapija parazitskih infekcija divljači, naša iskustva. in 33. Savetovanje veterinara Srbije, Zlatibor, 8 - 11. septembar 2022. 2022;:168-173.
https://hdl.handle.net/21.15107/rcub_veterinar_3343 .

Trailović, Saša M., Marinković, Darko, "Farmakoterapija parazitskih infekcija divljači, naša iskustva" in 33. Savetovanje veterinara Srbije, Zlatibor, 8 - 11. septembar 2022 (2022):168-173,
https://hdl.handle.net/21.15107/rcub_veterinar_3343 .

TY  - JOUR
AU  - Trailović, Saša M.
AU  - Rajković, Milan
AU  - Marjanović, Đorđe
AU  - Neveu, Cédric
AU  - Charvet, Claude L.
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2232
AB  - Parascaris sp. is the only ascarid parasitic nematode in equids and one of the most threatening infectious organisms in horses. Only a limited number of compounds are available for treatment of horse helminthiasis, and Parascaris sp. worms have developed resistance to the three major anthelmintic families. In order to overcome the appearance of resistance, there is an urgent need for new therapeutic strategies. The active ingredients of herbal essential oils are potentially effective antiparasitic drugs. Carvacrol is one of the principal chemicals of essential oil from Origanum, Thymus, Coridothymus, Thymbra, Satureja and Lippia herbs. However, the antiparasitic mode of action of carvacrol is poorly understood. Here, the objective of the work was to characterize the activity of carvacrol on Parascaris sp. nicotinic acetylcholine receptor (nAChR) function both in vivo with the use of worm neuromuscular flap preparations and in vitro with two-electrode voltage-clamp electrophysiology on nAChRs expressed in Xenopus oocytes. We developed a neuromuscular contraction assay for Parascaris body flaps and obtained acetylcholine concentration-dependent contraction responses. Strikingly, we observed that 300 µM carvacrol fully and irreversibly abolished Parascaris sp. muscle contractions elicited by acetylcholine. Similarly, carvacrol antagonized acetylcholine-induced currents from both the nicotine-sensitive AChR and the morantel-sensitive AChR subtypes. Thus, we show for the first time that body muscle flap preparation is a tractable approach to investigating the pharmacology of Parascaris sp. neuromuscular system. Our results suggest an intriguing mode of action for carvacrol, being a potent antagonist of muscle nAChRs of Parascaris sp. worms, which may account for its antiparasitic potency.
T2  - Pharmaceuticals
T1  - Action of Carvacrol on Parascaris sp. and Antagonistic Effect on Nicotinic Acetylcholine Receptors
VL  - 14
IS  - 6
SP  - 505
DO  - 10.3390/ph14060505
ER  - 

@article{
author = "Trailović, Saša M. and Rajković, Milan and Marjanović, Đorđe and Neveu, Cédric and Charvet, Claude L.",
year = "2021",
abstract = "Parascaris sp. is the only ascarid parasitic nematode in equids and one of the most threatening infectious organisms in horses. Only a limited number of compounds are available for treatment of horse helminthiasis, and Parascaris sp. worms have developed resistance to the three major anthelmintic families. In order to overcome the appearance of resistance, there is an urgent need for new therapeutic strategies. The active ingredients of herbal essential oils are potentially effective antiparasitic drugs. Carvacrol is one of the principal chemicals of essential oil from Origanum, Thymus, Coridothymus, Thymbra, Satureja and Lippia herbs. However, the antiparasitic mode of action of carvacrol is poorly understood. Here, the objective of the work was to characterize the activity of carvacrol on Parascaris sp. nicotinic acetylcholine receptor (nAChR) function both in vivo with the use of worm neuromuscular flap preparations and in vitro with two-electrode voltage-clamp electrophysiology on nAChRs expressed in Xenopus oocytes. We developed a neuromuscular contraction assay for Parascaris body flaps and obtained acetylcholine concentration-dependent contraction responses. Strikingly, we observed that 300 µM carvacrol fully and irreversibly abolished Parascaris sp. muscle contractions elicited by acetylcholine. Similarly, carvacrol antagonized acetylcholine-induced currents from both the nicotine-sensitive AChR and the morantel-sensitive AChR subtypes. Thus, we show for the first time that body muscle flap preparation is a tractable approach to investigating the pharmacology of Parascaris sp. neuromuscular system. Our results suggest an intriguing mode of action for carvacrol, being a potent antagonist of muscle nAChRs of Parascaris sp. worms, which may account for its antiparasitic potency.",
journal = "Pharmaceuticals",
title = "Action of Carvacrol on Parascaris sp. and Antagonistic Effect on Nicotinic Acetylcholine Receptors",
volume = "14",
number = "6",
pages = "505",
doi = "10.3390/ph14060505"
}

Trailović, S. M., Rajković, M., Marjanović, Đ., Neveu, C.,& Charvet, C. L.. (2021). Action of Carvacrol on Parascaris sp. and Antagonistic Effect on Nicotinic Acetylcholine Receptors. in Pharmaceuticals, 14(6), 505.
https://doi.org/10.3390/ph14060505

Trailović SM, Rajković M, Marjanović Đ, Neveu C, Charvet CL. Action of Carvacrol on Parascaris sp. and Antagonistic Effect on Nicotinic Acetylcholine Receptors. in Pharmaceuticals. 2021;14(6):505.
doi:10.3390/ph14060505 .

Trailović, Saša M., Rajković, Milan, Marjanović, Đorđe, Neveu, Cédric, Charvet, Claude L., "Action of Carvacrol on Parascaris sp. and Antagonistic Effect on Nicotinic Acetylcholine Receptors" in Pharmaceuticals, 14, no. 6 (2021):505,
https://doi.org/10.3390/ph14060505 . .

TY  - CONF
AU  - Marjanović, Đorđe S.
AU  - Rajković, Milan
AU  - Neveu, Cédric
AU  - Trailović, Saša
AU  - Charvet, Claude
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3417
AB  - Parascaris sp. is the only ascarid parasitic nematode in equids and one of the most threatening infectious organisms in horses. Only a limited number of compounds are available for treatment of horse helminthiasis and Parascaris sp. worms have developed resistance to the three major anthelmintic families. In order to overcome the appearance of resistance, there is an urgent need for new therapeutic strategies. The active ingredients of herbal essential oils are potentially effective antiparasitic drugs. Carvacrol is one of the principal chemical of essential oil from Origanum, Thymus, Coridothymus, Thymbra, Satureja and Lippia herbs. However the antiparasitic mode of action of carvacrol is poorly understood so far. Here, the objective of the work was to characterize and investigate the activity of carvacrol on Parascaris sp. nicotinic acetylcholine receptors (AChRs) function both in vivo with the use of worm neuro-muscular flap preparations and in vitro with elecrophysiology on nAChRs expressed in Xenopus oocytes. Strikingly, we observed that carvacrol fully abolished Parascaris sp. muscle contractions elicited by acetylcholine. Conversely, carvacrol inhibited acetylcholine-induced currents on both the nicotine-sentitive AChR and the morantel-sensitive AChR subtypes. Thus, we show for the first time that the body muscle flap preparation is a tractable approach to investigate the pharmacology of Parascaris sp. neuro-muscular system. Our results suggest an intriguing mode of action for carvacrol being a potent antagonist of muscle nAChRs of Parascaris sp. worms which may account for its antiparasitic potency.
C3  - 28-th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP 2021), Dublin, 2021
T1  - Antagonistic effect of carvacrol on nicotinic acetylcholine receptors from the horse parasite Parascaris sp.
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3417
ER  - 

@conference{
author = "Marjanović, Đorđe S. and Rajković, Milan and Neveu, Cédric and Trailović, Saša and Charvet, Claude",
year = "2021",
abstract = "Parascaris sp. is the only ascarid parasitic nematode in equids and one of the most threatening infectious organisms in horses. Only a limited number of compounds are available for treatment of horse helminthiasis and Parascaris sp. worms have developed resistance to the three major anthelmintic families. In order to overcome the appearance of resistance, there is an urgent need for new therapeutic strategies. The active ingredients of herbal essential oils are potentially effective antiparasitic drugs. Carvacrol is one of the principal chemical of essential oil from Origanum, Thymus, Coridothymus, Thymbra, Satureja and Lippia herbs. However the antiparasitic mode of action of carvacrol is poorly understood so far. Here, the objective of the work was to characterize and investigate the activity of carvacrol on Parascaris sp. nicotinic acetylcholine receptors (AChRs) function both in vivo with the use of worm neuro-muscular flap preparations and in vitro with elecrophysiology on nAChRs expressed in Xenopus oocytes. Strikingly, we observed that carvacrol fully abolished Parascaris sp. muscle contractions elicited by acetylcholine. Conversely, carvacrol inhibited acetylcholine-induced currents on both the nicotine-sentitive AChR and the morantel-sensitive AChR subtypes. Thus, we show for the first time that the body muscle flap preparation is a tractable approach to investigate the pharmacology of Parascaris sp. neuro-muscular system. Our results suggest an intriguing mode of action for carvacrol being a potent antagonist of muscle nAChRs of Parascaris sp. worms which may account for its antiparasitic potency.",
journal = "28-th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP 2021), Dublin, 2021",
title = "Antagonistic effect of carvacrol on nicotinic acetylcholine receptors from the horse parasite Parascaris sp.",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3417"
}

Marjanović, Đ. S., Rajković, M., Neveu, C., Trailović, S.,& Charvet, C.. (2021). Antagonistic effect of carvacrol on nicotinic acetylcholine receptors from the horse parasite Parascaris sp.. in 28-th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP 2021), Dublin, 2021.
https://hdl.handle.net/21.15107/rcub_veterinar_3417

Marjanović ĐS, Rajković M, Neveu C, Trailović S, Charvet C. Antagonistic effect of carvacrol on nicotinic acetylcholine receptors from the horse parasite Parascaris sp.. in 28-th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP 2021), Dublin, 2021. 2021;.
https://hdl.handle.net/21.15107/rcub_veterinar_3417 .

Marjanović, Đorđe S., Rajković, Milan, Neveu, Cédric, Trailović, Saša, Charvet, Claude, "Antagonistic effect of carvacrol on nicotinic acetylcholine receptors from the horse parasite Parascaris sp." in 28-th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP 2021), Dublin, 2021 (2021),
https://hdl.handle.net/21.15107/rcub_veterinar_3417 .

TY  - JOUR
AU  - Marjanović, Đorđe
AU  - Trailović, Saša M.
AU  - Milovanović, Mirjana
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2040
AB  - Resistance of parasitic nematodes to anthelmintic drugs is a growing problem in human and veterinary medicine. The molecular mechanisms by which nematodes become resistant are different, but certainly one of the possible processes involves changing the drug binding site on the specific receptor. The significance of changes in individual subtypes of nicotinic acetylcholine receptors (nAChRs) for the development of resistance has not been clarified in detail. This study investigates the interaction of antinematodal drugs, agonist of different types of nAChRs and carvacrol with gamma aminobutyric acid (GABA) on the contractions of parasitic nematode A. suum. In our study, GABA (3 μM) produced significant increase of contractile EC50 value for pyrantel, and nonsignificant for bephenium and morantel, from 8.44 to 28.11 nM, 0.62 to 0.96 µM, and 3.72 to 5.69 nM, respectively. On the other hand, the maximal contractile effect (Rmax) did not change in the presence of GABA. However, when A. summ muscle flaps were incubated with GABA 3 μM and carvacrol 100 μM, the EC50 value of pyrantel, bephenium, and morantel was increased significantly to 44.62 nM, 1.40 μM, and nonsignificantly to 7.94 nM, respectively. Furthermore, Rmax decreased by 70, 60, and 65%. Presented results indicate that the combined use of GABA receptor agonists and nicotinic receptor antagonists can effectively inhibit the neuromuscular system of nematodes, even when one of the nicotinic receptor subtypes is dysfunctional, due to the potential development of resistance.
PB  - Exeley, Inc.
T2  - Journal of Nematology
T1  - Interaction of agonists of a different subtype of the nAChR and carvacrol with GABA in Ascaris suum somatic muscle contractions
VL  - 53
SP  - e2021-022
DO  - 10.21307/jofnem-2021-022
ER  - 

@article{
author = "Marjanović, Đorđe and Trailović, Saša M. and Milovanović, Mirjana",
year = "2021",
abstract = "Resistance of parasitic nematodes to anthelmintic drugs is a growing problem in human and veterinary medicine. The molecular mechanisms by which nematodes become resistant are different, but certainly one of the possible processes involves changing the drug binding site on the specific receptor. The significance of changes in individual subtypes of nicotinic acetylcholine receptors (nAChRs) for the development of resistance has not been clarified in detail. This study investigates the interaction of antinematodal drugs, agonist of different types of nAChRs and carvacrol with gamma aminobutyric acid (GABA) on the contractions of parasitic nematode A. suum. In our study, GABA (3 μM) produced significant increase of contractile EC50 value for pyrantel, and nonsignificant for bephenium and morantel, from 8.44 to 28.11 nM, 0.62 to 0.96 µM, and 3.72 to 5.69 nM, respectively. On the other hand, the maximal contractile effect (Rmax) did not change in the presence of GABA. However, when A. summ muscle flaps were incubated with GABA 3 μM and carvacrol 100 μM, the EC50 value of pyrantel, bephenium, and morantel was increased significantly to 44.62 nM, 1.40 μM, and nonsignificantly to 7.94 nM, respectively. Furthermore, Rmax decreased by 70, 60, and 65%. Presented results indicate that the combined use of GABA receptor agonists and nicotinic receptor antagonists can effectively inhibit the neuromuscular system of nematodes, even when one of the nicotinic receptor subtypes is dysfunctional, due to the potential development of resistance.",
publisher = "Exeley, Inc.",
journal = "Journal of Nematology",
title = "Interaction of agonists of a different subtype of the nAChR and carvacrol with GABA in Ascaris suum somatic muscle contractions",
volume = "53",
pages = "e2021-022",
doi = "10.21307/jofnem-2021-022"
}

Marjanović, Đ., Trailović, S. M.,& Milovanović, M.. (2021). Interaction of agonists of a different subtype of the nAChR and carvacrol with GABA in Ascaris suum somatic muscle contractions. in Journal of Nematology
Exeley, Inc.., 53, e2021-022.
https://doi.org/10.21307/jofnem-2021-022

Marjanović Đ, Trailović SM, Milovanović M. Interaction of agonists of a different subtype of the nAChR and carvacrol with GABA in Ascaris suum somatic muscle contractions. in Journal of Nematology. 2021;53:e2021-022.
doi:10.21307/jofnem-2021-022 .

Marjanović, Đorđe, Trailović, Saša M., Milovanović, Mirjana, "Interaction of agonists of a different subtype of the nAChR and carvacrol with GABA in Ascaris suum somatic muscle contractions" in Journal of Nematology, 53 (2021):e2021-022,
https://doi.org/10.21307/jofnem-2021-022 . .

TY  - JOUR
AU  - Stevanovic, Strahinja
AU  - Marjanović, Đorđe
AU  - Trailović, Saša M.
AU  - Zdravković, Nemanja
AU  - Perdih, Andrej
AU  - Nikolic, Katarina
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2016
AB  - Parasitic infections are a widespread health problem and research of novel anthelmintic compounds is of the utmost importance. In this study we performed a virtual screening campaign by coupling ligand-based pharmacophore, homology modeling and molecular docking. The virtual screening campaign was conducted using a joined pool of the Drugbank database and a library of purchasable compounds in order to identify drug like compounds with similar pharmacological activity. Our aim was to identify compounds with a potential antihelmintic modulatory effect on nicotinic acetylcholine receptors (nAChR). We derived a 3D pharmacophore model based on the chemical features of known Ascaris suum nAChR modulators. To evaluate the in silico predictions, we tested selected hit compounds in contraction assays using somatic muscle flaps of the Ascaris suum neuromuscular tissue. We tested the modulatory effects of GSK575594A, diazepam and flumazenil hit compounds on nematode contractions induced by acetyl choline (ACh). The compound GSK575594A (3 μM) increased the Emax by 21 % with the EC50 dose ratio of 0.96. Diazepam (100 μM) decreased the Emax by 15 % (1.11 g to 0.95 g) with the EC50 ratio of 1.42 (shifted to the left from 11.25 to 7.93). Flumazenil decreased the EC50 value (from 11.22 μM to 4.88 μM) value showing dose ratio of 2.30, and increased the Emax by 4 % (from 1.54 g to 1.59 g). The observed biological activity was rationalized by molecular docking calculations. Docking scores were calculated against several binding sites within the Ascaris suum homology model. We constructed the homology model using the ACR-16 subunit sequence. The compound GSK575594A showed strong affinity for the intersubunit allosteric binding site within the nAChR transmembrane domain. The binding modes of diazepam and flumazenil suggest that these compounds have a comparable affinity for orthosteric and allosteric nAChR binding sites. The selected hit compounds displayed potential for further optimization as lead compounds. Therefore, such compounds may be useful in neutralizing the growing resistance of parasites to drugs, either alone or in combination with existing conventional anthelmintics.
PB  - Elsevier
T2  - Molecular and Biochemical Parasitology
T1  - Potential modulating effect of the Ascaris suum nicotinic acetylcholine receptor (nAChR) by compounds GSK575594A, diazepam and flumazenil discovered by structure-based virtual screening approach
VL  - 242
DO  - 10.1016/j.molbiopara.2021.111350
ER  - 

@article{
author = "Stevanovic, Strahinja and Marjanović, Đorđe and Trailović, Saša M. and Zdravković, Nemanja and Perdih, Andrej and Nikolic, Katarina",
year = "2021",
abstract = "Parasitic infections are a widespread health problem and research of novel anthelmintic compounds is of the utmost importance. In this study we performed a virtual screening campaign by coupling ligand-based pharmacophore, homology modeling and molecular docking. The virtual screening campaign was conducted using a joined pool of the Drugbank database and a library of purchasable compounds in order to identify drug like compounds with similar pharmacological activity. Our aim was to identify compounds with a potential antihelmintic modulatory effect on nicotinic acetylcholine receptors (nAChR). We derived a 3D pharmacophore model based on the chemical features of known Ascaris suum nAChR modulators. To evaluate the in silico predictions, we tested selected hit compounds in contraction assays using somatic muscle flaps of the Ascaris suum neuromuscular tissue. We tested the modulatory effects of GSK575594A, diazepam and flumazenil hit compounds on nematode contractions induced by acetyl choline (ACh). The compound GSK575594A (3 μM) increased the Emax by 21 % with the EC50 dose ratio of 0.96. Diazepam (100 μM) decreased the Emax by 15 % (1.11 g to 0.95 g) with the EC50 ratio of 1.42 (shifted to the left from 11.25 to 7.93). Flumazenil decreased the EC50 value (from 11.22 μM to 4.88 μM) value showing dose ratio of 2.30, and increased the Emax by 4 % (from 1.54 g to 1.59 g). The observed biological activity was rationalized by molecular docking calculations. Docking scores were calculated against several binding sites within the Ascaris suum homology model. We constructed the homology model using the ACR-16 subunit sequence. The compound GSK575594A showed strong affinity for the intersubunit allosteric binding site within the nAChR transmembrane domain. The binding modes of diazepam and flumazenil suggest that these compounds have a comparable affinity for orthosteric and allosteric nAChR binding sites. The selected hit compounds displayed potential for further optimization as lead compounds. Therefore, such compounds may be useful in neutralizing the growing resistance of parasites to drugs, either alone or in combination with existing conventional anthelmintics.",
publisher = "Elsevier",
journal = "Molecular and Biochemical Parasitology",
title = "Potential modulating effect of the Ascaris suum nicotinic acetylcholine receptor (nAChR) by compounds GSK575594A, diazepam and flumazenil discovered by structure-based virtual screening approach",
volume = "242",
doi = "10.1016/j.molbiopara.2021.111350"
}

Stevanovic, S., Marjanović, Đ., Trailović, S. M., Zdravković, N., Perdih, A.,& Nikolic, K.. (2021). Potential modulating effect of the Ascaris suum nicotinic acetylcholine receptor (nAChR) by compounds GSK575594A, diazepam and flumazenil discovered by structure-based virtual screening approach. in Molecular and Biochemical Parasitology
Elsevier., 242.
https://doi.org/10.1016/j.molbiopara.2021.111350

Stevanovic S, Marjanović Đ, Trailović SM, Zdravković N, Perdih A, Nikolic K. Potential modulating effect of the Ascaris suum nicotinic acetylcholine receptor (nAChR) by compounds GSK575594A, diazepam and flumazenil discovered by structure-based virtual screening approach. in Molecular and Biochemical Parasitology. 2021;242.
doi:10.1016/j.molbiopara.2021.111350 .

Stevanovic, Strahinja, Marjanović, Đorđe, Trailović, Saša M., Zdravković, Nemanja, Perdih, Andrej, Nikolic, Katarina, "Potential modulating effect of the Ascaris suum nicotinic acetylcholine receptor (nAChR) by compounds GSK575594A, diazepam and flumazenil discovered by structure-based virtual screening approach" in Molecular and Biochemical Parasitology, 242 (2021),
https://doi.org/10.1016/j.molbiopara.2021.111350 . .

TY  - JOUR
AU  - Marjanović, Đorđe
AU  - Zdravković, Nemanja
AU  - Milovanović, Mirjana
AU  - Nedeljković-Trailović, Jelena
AU  - Robertson, Alan P.
AU  - Todorović, Zoran
AU  - Trailović, Saša
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1807
AB  - The neuromuscular system of parasitic nematodes has proven to be an efficient pharmacological target for antihelmintics. Some of the most frequently used antiparasitic drugs are agonists or antagonists of nicotinic acetylcholine receptors (nAChRs). The antinematodal mechanism of action of carvacrol involves the inhibition of parasite muscle contraction. We have examined the interaction of carvacrol with antinematodal drugs that are agonists of different subtypes of nAChRs and monepantel, which is a non-competitive antagonist of this receptor in A. suum. Additionally, we investigated the effect of carvacrol on the muscle type of nAChRs in the mammalian host. As orthosteric agonists of nAChR, pyrantel, morantel and befinijum lead to dose-dependent contractions of the neuromuscular preparation of Ascaris suum. Carvacrol 100 mu M decreased the E-max of pyrantel, morantel and bephenium by 29%, 39% and 12 %, 39 % and 12 % respectively. The EC50 ratio was 3.43, 2.95 and 2.47 for pyrantel, morantel and bephinium, respectively. Carvacrol 300 u mu M reduces the E-max of pyrantel, morantel and bephenium by 71%, 80% and 75 %, 80 % and 75 % respectively. The EC50 ratio for pyrantel, morantel and bephenium was 3.88, 3.19 and 4.83 respectively. Furthermore, carvacrol enhances the inhibitory effect of monepantel on A. mum contractions, which may have an effective clinical application. On the other hand, tested concentrations of carvacrol did not significantly affect the EFS-induced contractions of the rat diaphragm, indicating a lack of interaction with the postsynaptic nAChR at the muscle end plate in mammals, but the highest concentration (300 mu M) caused a clear tetanic fade. Carvacrol exhibited a time and dose-dependent effect on the Rota-rod performances of rats with a high value of the ED50 (421.6 mg/kg). In our research, carvacrol dominantly exhibited characteristics of a non-competitive antagonist of nAChR in A. suum, and enhances the inhibitory effect of monepantel. The combination of monepantel and carvacrol may be clinically very effective, and the carvacrol molecule itself can be used as a promising platform for the development of new anthelmintic drugs.
PB  - Elsevier, Amsterdam
T2  - Veterinary Parasitology
T1  - Carvacrol acts as a potent selective antagonist of different types of nicotinic acetylcholine receptors and enhances the effect of monepantel in the parasitic nematode Ascaris suum
VL  - 278
SP  - UNSP 109031
DO  - 10.1016/j.vetpar.2020.109031
ER  - 

@article{
author = "Marjanović, Đorđe and Zdravković, Nemanja and Milovanović, Mirjana and Nedeljković-Trailović, Jelena and Robertson, Alan P. and Todorović, Zoran and Trailović, Saša",
year = "2020",
abstract = "The neuromuscular system of parasitic nematodes has proven to be an efficient pharmacological target for antihelmintics. Some of the most frequently used antiparasitic drugs are agonists or antagonists of nicotinic acetylcholine receptors (nAChRs). The antinematodal mechanism of action of carvacrol involves the inhibition of parasite muscle contraction. We have examined the interaction of carvacrol with antinematodal drugs that are agonists of different subtypes of nAChRs and monepantel, which is a non-competitive antagonist of this receptor in A. suum. Additionally, we investigated the effect of carvacrol on the muscle type of nAChRs in the mammalian host. As orthosteric agonists of nAChR, pyrantel, morantel and befinijum lead to dose-dependent contractions of the neuromuscular preparation of Ascaris suum. Carvacrol 100 mu M decreased the E-max of pyrantel, morantel and bephenium by 29%, 39% and 12 %, 39 % and 12 % respectively. The EC50 ratio was 3.43, 2.95 and 2.47 for pyrantel, morantel and bephinium, respectively. Carvacrol 300 u mu M reduces the E-max of pyrantel, morantel and bephenium by 71%, 80% and 75 %, 80 % and 75 % respectively. The EC50 ratio for pyrantel, morantel and bephenium was 3.88, 3.19 and 4.83 respectively. Furthermore, carvacrol enhances the inhibitory effect of monepantel on A. mum contractions, which may have an effective clinical application. On the other hand, tested concentrations of carvacrol did not significantly affect the EFS-induced contractions of the rat diaphragm, indicating a lack of interaction with the postsynaptic nAChR at the muscle end plate in mammals, but the highest concentration (300 mu M) caused a clear tetanic fade. Carvacrol exhibited a time and dose-dependent effect on the Rota-rod performances of rats with a high value of the ED50 (421.6 mg/kg). In our research, carvacrol dominantly exhibited characteristics of a non-competitive antagonist of nAChR in A. suum, and enhances the inhibitory effect of monepantel. The combination of monepantel and carvacrol may be clinically very effective, and the carvacrol molecule itself can be used as a promising platform for the development of new anthelmintic drugs.",
publisher = "Elsevier, Amsterdam",
journal = "Veterinary Parasitology",
title = "Carvacrol acts as a potent selective antagonist of different types of nicotinic acetylcholine receptors and enhances the effect of monepantel in the parasitic nematode Ascaris suum",
volume = "278",
pages = "UNSP 109031",
doi = "10.1016/j.vetpar.2020.109031"
}

Marjanović, Đ., Zdravković, N., Milovanović, M., Nedeljković-Trailović, J., Robertson, A. P., Todorović, Z.,& Trailović, S.. (2020). Carvacrol acts as a potent selective antagonist of different types of nicotinic acetylcholine receptors and enhances the effect of monepantel in the parasitic nematode Ascaris suum. in Veterinary Parasitology
Elsevier, Amsterdam., 278, UNSP 109031.
https://doi.org/10.1016/j.vetpar.2020.109031

Marjanović Đ, Zdravković N, Milovanović M, Nedeljković-Trailović J, Robertson AP, Todorović Z, Trailović S. Carvacrol acts as a potent selective antagonist of different types of nicotinic acetylcholine receptors and enhances the effect of monepantel in the parasitic nematode Ascaris suum. in Veterinary Parasitology. 2020;278:UNSP 109031.
doi:10.1016/j.vetpar.2020.109031 .

Marjanović, Đorđe, Zdravković, Nemanja, Milovanović, Mirjana, Nedeljković-Trailović, Jelena, Robertson, Alan P., Todorović, Zoran, Trailović, Saša, "Carvacrol acts as a potent selective antagonist of different types of nicotinic acetylcholine receptors and enhances the effect of monepantel in the parasitic nematode Ascaris suum" in Veterinary Parasitology, 278 (2020):UNSP 109031,
https://doi.org/10.1016/j.vetpar.2020.109031 . .

TY  - JOUR
AU  - Atanasković, Iva
AU  - Marjanović, Đorđe
AU  - Trailović, Saša
AU  - Fira, Đorđe
AU  - Stanković, Slaviša
AU  - Lozo, Jelena
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1973
AB  - Natural isolates coming from chemically untreated environmental samples can be a source of new nematocidal bacterial strains. Little is known about the abundance of such isolates; the diversity of nematocidal proteins that they produce, or how their nematocidal activity changes in a growth phase-dependent manner. In this study, a collection of 17 Bacillus thuringiensis (Bt) natural isolates was screened for nematocidal activity and the presence of nematocidal genes (cry1, cry5, cry6, cry12, cry13, cry14, and cry21). Most strains were positive for cry1 and at least one other cry gene. Further, nematocidal activity against Caenorhabditis elegans was detected in 82.35% of strains. This percentage is significantly higher than percentages found in previously reported studies on similar strain collections. We next sought to investigate the correlation between bacterial growth phase and nematocidal activity. All strains clustered into two growth phase-dependent nematocidal activity profiles. 28.57% of nematocidal strains were active from the exponential and throughout the stationary phase. 42.85% of them were active only in the exponential phase. Strains active only in the stationary phase were not observed. A combination of strains active in different growth phases was then used to investigate the possibility of killing entire nematode populations. Complete clearance of nematodes was accomplished after two days of incubation when a mixed culture of two such strains (SS-9.4 and SS-16.2) was used. Therefore, combining Bt strains, which are nematocidal in different growth phases, could ameliorate their application in the biological control of nematodes.
PB  - Taylor and Francis Ltd.
T2  - Biocontrol Science and Technology
T1  - Growth phase-dependent nematocidal activity of Bacillus thuringiensis strains from natural samples
VL  - 30
IS  - 11
SP  - 1199
EP  - 1211
DO  - 10.1080/09583157.2020.1802698
ER  - 

@article{
author = "Atanasković, Iva and Marjanović, Đorđe and Trailović, Saša and Fira, Đorđe and Stanković, Slaviša and Lozo, Jelena",
year = "2020",
abstract = "Natural isolates coming from chemically untreated environmental samples can be a source of new nematocidal bacterial strains. Little is known about the abundance of such isolates; the diversity of nematocidal proteins that they produce, or how their nematocidal activity changes in a growth phase-dependent manner. In this study, a collection of 17 Bacillus thuringiensis (Bt) natural isolates was screened for nematocidal activity and the presence of nematocidal genes (cry1, cry5, cry6, cry12, cry13, cry14, and cry21). Most strains were positive for cry1 and at least one other cry gene. Further, nematocidal activity against Caenorhabditis elegans was detected in 82.35% of strains. This percentage is significantly higher than percentages found in previously reported studies on similar strain collections. We next sought to investigate the correlation between bacterial growth phase and nematocidal activity. All strains clustered into two growth phase-dependent nematocidal activity profiles. 28.57% of nematocidal strains were active from the exponential and throughout the stationary phase. 42.85% of them were active only in the exponential phase. Strains active only in the stationary phase were not observed. A combination of strains active in different growth phases was then used to investigate the possibility of killing entire nematode populations. Complete clearance of nematodes was accomplished after two days of incubation when a mixed culture of two such strains (SS-9.4 and SS-16.2) was used. Therefore, combining Bt strains, which are nematocidal in different growth phases, could ameliorate their application in the biological control of nematodes.",
publisher = "Taylor and Francis Ltd.",
journal = "Biocontrol Science and Technology",
title = "Growth phase-dependent nematocidal activity of Bacillus thuringiensis strains from natural samples",
volume = "30",
number = "11",
pages = "1199-1211",
doi = "10.1080/09583157.2020.1802698"
}

Atanasković, I., Marjanović, Đ., Trailović, S., Fira, Đ., Stanković, S.,& Lozo, J.. (2020). Growth phase-dependent nematocidal activity of Bacillus thuringiensis strains from natural samples. in Biocontrol Science and Technology
Taylor and Francis Ltd.., 30(11), 1199-1211.
https://doi.org/10.1080/09583157.2020.1802698

Atanasković I, Marjanović Đ, Trailović S, Fira Đ, Stanković S, Lozo J. Growth phase-dependent nematocidal activity of Bacillus thuringiensis strains from natural samples. in Biocontrol Science and Technology. 2020;30(11):1199-1211.
doi:10.1080/09583157.2020.1802698 .

Atanasković, Iva, Marjanović, Đorđe, Trailović, Saša, Fira, Đorđe, Stanković, Slaviša, Lozo, Jelena, "Growth phase-dependent nematocidal activity of Bacillus thuringiensis strains from natural samples" in Biocontrol Science and Technology, 30, no. 11 (2020):1199-1211,
https://doi.org/10.1080/09583157.2020.1802698 . .

TY  - CONF
AU  - Marjanović, Đorđe
AU  - Zdravković, Nemanja
AU  - Pavlović, Marija
AU  - Nešić, Ksenija
AU  - Savić Radovanović, Radoslava
AU  - Trailović, Saša
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3311
AB  - Introduction: Modern antiparasitic pharmacotherapy faces several important challenges. An 
increasing number of reports refer to the resistance of parasitic nematodes to conventional 
antiparasitic drugs, while dose increasing manifests their toxicity. Particularly important for 
veterinary medicine is the fact that most antiparasitic drugs require a long withdrawal period 
after application in animals whose tissues are intended for human consumption. 
Aims: To examine the potential antinematodic effect of selected monoterpenoid and 
diterpenoid active principles of essential vegetable oils and determine the mechanism by 
which they achieve antiparasitic effects.
Materials and Methods: Investigation of the interaction of active principles of essential 
vegetable oils with cholinergic and GABA-ergic nematode receptors on neuromuscular 
preparation Ascaris suum and Caenorhabidis elegans culture.
Results: The terpenoids alpha-pinene, p-cymene, carveol, carnosol and cinnamaldehyde do 
not show any pharmacological effects on the contractions of the neuromuscular preparation A. 
suum. The monoterpenoid carvacrol exhibits the properties of the competitive / noncompetitive nicotinic acetylcholine receptor (nAChR) antagonist A. suum and effectively and 
significantly inhibits neuromuscular contractions caused by increasing acetylcholine 
concentrations. - carvacrol does not alter the EC50 value of acetylcholine The tested active 
principles of essential oils in our tests showed a specific and unique mechanism of action that 
potentially enables them high efficacy, even in nematodes resistant to classical anthelmintics.
Conclusion: Based on our results, carvacrol, carveol, menthol and thymol are very serious 
candidates for independent use in antinematode therapy or use in combination with drugs 
agonists of GABA receptor nematodes.
PB  - University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca
C3  - 19th International Conference Life Sciences for Sustainable Development, Cluj-Napoca, Romania, 24th - 25th September 2020
T1  - Antiparsitic effect of some active components of essential oils
SP  - 287
EP  - 287
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3311
ER  - 

@conference{
author = "Marjanović, Đorđe and Zdravković, Nemanja and Pavlović, Marija and Nešić, Ksenija and Savić Radovanović, Radoslava and Trailović, Saša",
year = "2020",
abstract = "Introduction: Modern antiparasitic pharmacotherapy faces several important challenges. An 
increasing number of reports refer to the resistance of parasitic nematodes to conventional 
antiparasitic drugs, while dose increasing manifests their toxicity. Particularly important for 
veterinary medicine is the fact that most antiparasitic drugs require a long withdrawal period 
after application in animals whose tissues are intended for human consumption. 
Aims: To examine the potential antinematodic effect of selected monoterpenoid and 
diterpenoid active principles of essential vegetable oils and determine the mechanism by 
which they achieve antiparasitic effects.
Materials and Methods: Investigation of the interaction of active principles of essential 
vegetable oils with cholinergic and GABA-ergic nematode receptors on neuromuscular 
preparation Ascaris suum and Caenorhabidis elegans culture.
Results: The terpenoids alpha-pinene, p-cymene, carveol, carnosol and cinnamaldehyde do 
not show any pharmacological effects on the contractions of the neuromuscular preparation A. 
suum. The monoterpenoid carvacrol exhibits the properties of the competitive / noncompetitive nicotinic acetylcholine receptor (nAChR) antagonist A. suum and effectively and 
significantly inhibits neuromuscular contractions caused by increasing acetylcholine 
concentrations. - carvacrol does not alter the EC50 value of acetylcholine The tested active 
principles of essential oils in our tests showed a specific and unique mechanism of action that 
potentially enables them high efficacy, even in nematodes resistant to classical anthelmintics.
Conclusion: Based on our results, carvacrol, carveol, menthol and thymol are very serious 
candidates for independent use in antinematode therapy or use in combination with drugs 
agonists of GABA receptor nematodes.",
publisher = "University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca",
journal = "19th International Conference Life Sciences for Sustainable Development, Cluj-Napoca, Romania, 24th - 25th September 2020",
title = "Antiparsitic effect of some active components of essential oils",
pages = "287-287",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3311"
}

Marjanović, Đ., Zdravković, N., Pavlović, M., Nešić, K., Savić Radovanović, R.,& Trailović, S.. (2020). Antiparsitic effect of some active components of essential oils. in 19th International Conference Life Sciences for Sustainable Development, Cluj-Napoca, Romania, 24th - 25th September 2020
University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca., 287-287.
https://hdl.handle.net/21.15107/rcub_veterinar_3311

Marjanović Đ, Zdravković N, Pavlović M, Nešić K, Savić Radovanović R, Trailović S. Antiparsitic effect of some active components of essential oils. in 19th International Conference Life Sciences for Sustainable Development, Cluj-Napoca, Romania, 24th - 25th September 2020. 2020;:287-287.
https://hdl.handle.net/21.15107/rcub_veterinar_3311 .

Marjanović, Đorđe, Zdravković, Nemanja, Pavlović, Marija, Nešić, Ksenija, Savić Radovanović, Radoslava, Trailović, Saša, "Antiparsitic effect of some active components of essential oils" in 19th International Conference Life Sciences for Sustainable Development, Cluj-Napoca, Romania, 24th - 25th September 2020 (2020):287-287,
https://hdl.handle.net/21.15107/rcub_veterinar_3311 .

TY  - CONF
AU  - Marjanović, Đorđe
AU  - Zdravković, Nemanja
AU  - Pavlović, Marija
AU  - Nešić, Ksenija
AU  - Savić Radovanović, Radoslava
AU  - Trailović, Saša
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3312
AB  - Modern antiparasitic pharmacotherapy faces several important challenges. An
increasing number of reports refer to the resistance of parasitic nematodes to conventional
antiparasitic drugs, while dose increasing manifests their toxicity. Particularly important for
veterinary medicine is the fact that most antiparasitic drugs require a long withdrawal period
after application in animals whose tissues are intended for human consumption.
PB  - University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca
C3  - 19th International Conference Life sciences for sustainable development, Cluj-Napoca, Romania, 24th - 25th September 2020
T1  - Antiparsitic effect of some active components of essential oils
SP  - 287
EP  - 287
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3312
ER  - 

@conference{
author = "Marjanović, Đorđe and Zdravković, Nemanja and Pavlović, Marija and Nešić, Ksenija and Savić Radovanović, Radoslava and Trailović, Saša",
year = "2020",
abstract = "Modern antiparasitic pharmacotherapy faces several important challenges. An
increasing number of reports refer to the resistance of parasitic nematodes to conventional
antiparasitic drugs, while dose increasing manifests their toxicity. Particularly important for
veterinary medicine is the fact that most antiparasitic drugs require a long withdrawal period
after application in animals whose tissues are intended for human consumption.",
publisher = "University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca",
journal = "19th International Conference Life sciences for sustainable development, Cluj-Napoca, Romania, 24th - 25th September 2020",
title = "Antiparsitic effect of some active components of essential oils",
pages = "287-287",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3312"
}

Marjanović, Đ., Zdravković, N., Pavlović, M., Nešić, K., Savić Radovanović, R.,& Trailović, S.. (2020). Antiparsitic effect of some active components of essential oils. in 19th International Conference Life sciences for sustainable development, Cluj-Napoca, Romania, 24th - 25th September 2020
University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca., 287-287.
https://hdl.handle.net/21.15107/rcub_veterinar_3312

Marjanović Đ, Zdravković N, Pavlović M, Nešić K, Savić Radovanović R, Trailović S. Antiparsitic effect of some active components of essential oils. in 19th International Conference Life sciences for sustainable development, Cluj-Napoca, Romania, 24th - 25th September 2020. 2020;:287-287.
https://hdl.handle.net/21.15107/rcub_veterinar_3312 .

Marjanović, Đorđe, Zdravković, Nemanja, Pavlović, Marija, Nešić, Ksenija, Savić Radovanović, Radoslava, Trailović, Saša, "Antiparsitic effect of some active components of essential oils" in 19th International Conference Life sciences for sustainable development, Cluj-Napoca, Romania, 24th - 25th September 2020 (2020):287-287,
https://hdl.handle.net/21.15107/rcub_veterinar_3312 .

TY  - JOUR
AU  - Rajković, Milan
AU  - Vučićević, Ivana
AU  - Vučićević, Miloš
AU  - Dosenović, Milan
AU  - Charvet, L. Claude
AU  - Resanović, Radmila
AU  - Trailović, Saša
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1696
AB  - We present a case of Ascaridia galli infection in laying hens on a farm in central Serbia. During the rearing period on litter, ascaridosis was diagnosed at 15 weeks of age by routine parasitological fecal examination. Pullets were treated with flubendazole for one week, and two weeks later the hens were moved to battery cages. The production results were within technological standards until the 23rd week and the medical health status was without any clinical symptoms. After that period weight loss began, the egg production dropped to 70% and eggs were of poor quality. Subsequently, severe feather pecking and an increase of mortality were reported. The postmortal examination showed severe anemia and several gross lesions in the liver, intestines, lungs, and kidneys. Different sizes of A. galli were found in the lumen of the duodenum and jejunum. Gross changes of the intestinal mucosa were present, such as a thickened intestinal wall with hemorrhagic spots, inflammation and necrotic patches. Histopathological examination showed marked changes in the intestines, liver and kidneys. All visible live parasites were collected and stored in Earles balanced salts, and females were used for in vitro susceptibility testing. Median lethal concentration (LC50) of piperazine, levamisole and carvacrol for A. galli was 119.7 mu M, 2.71 mu M and 3.26 mu M, were applied, respectively. Based on our results, it is likely that reinfection occurred after completed dehelmintization. In relation to the new circumstances and the regulation for laying hen welfare the deworming protocol should be changed in order to ensure successful dehelmintization. In order to prevent reinfection the treatment must be done at the end of the rearing period and thus be maximally effective.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Ascaridia galli infection in laying hens and the results of in vitro efficacy of levamisole, piperazine and carvacrol, whether is necessary to change the deworming protocols?
VL  - 69
IS  - 4
SP  - 414
EP  - 425
DO  - 10.2478/acve-2019-0035
ER  - 

@article{
author = "Rajković, Milan and Vučićević, Ivana and Vučićević, Miloš and Dosenović, Milan and Charvet, L. Claude and Resanović, Radmila and Trailović, Saša",
year = "2019",
abstract = "We present a case of Ascaridia galli infection in laying hens on a farm in central Serbia. During the rearing period on litter, ascaridosis was diagnosed at 15 weeks of age by routine parasitological fecal examination. Pullets were treated with flubendazole for one week, and two weeks later the hens were moved to battery cages. The production results were within technological standards until the 23rd week and the medical health status was without any clinical symptoms. After that period weight loss began, the egg production dropped to 70% and eggs were of poor quality. Subsequently, severe feather pecking and an increase of mortality were reported. The postmortal examination showed severe anemia and several gross lesions in the liver, intestines, lungs, and kidneys. Different sizes of A. galli were found in the lumen of the duodenum and jejunum. Gross changes of the intestinal mucosa were present, such as a thickened intestinal wall with hemorrhagic spots, inflammation and necrotic patches. Histopathological examination showed marked changes in the intestines, liver and kidneys. All visible live parasites were collected and stored in Earles balanced salts, and females were used for in vitro susceptibility testing. Median lethal concentration (LC50) of piperazine, levamisole and carvacrol for A. galli was 119.7 mu M, 2.71 mu M and 3.26 mu M, were applied, respectively. Based on our results, it is likely that reinfection occurred after completed dehelmintization. In relation to the new circumstances and the regulation for laying hen welfare the deworming protocol should be changed in order to ensure successful dehelmintization. In order to prevent reinfection the treatment must be done at the end of the rearing period and thus be maximally effective.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Ascaridia galli infection in laying hens and the results of in vitro efficacy of levamisole, piperazine and carvacrol, whether is necessary to change the deworming protocols?",
volume = "69",
number = "4",
pages = "414-425",
doi = "10.2478/acve-2019-0035"
}

Rajković, M., Vučićević, I., Vučićević, M., Dosenović, M., Charvet, L. C., Resanović, R.,& Trailović, S.. (2019). Ascaridia galli infection in laying hens and the results of in vitro efficacy of levamisole, piperazine and carvacrol, whether is necessary to change the deworming protocols?. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(4), 414-425.
https://doi.org/10.2478/acve-2019-0035

Rajković M, Vučićević I, Vučićević M, Dosenović M, Charvet LC, Resanović R, Trailović S. Ascaridia galli infection in laying hens and the results of in vitro efficacy of levamisole, piperazine and carvacrol, whether is necessary to change the deworming protocols?. in Acta Veterinaria-Beograd. 2019;69(4):414-425.
doi:10.2478/acve-2019-0035 .

Rajković, Milan, Vučićević, Ivana, Vučićević, Miloš, Dosenović, Milan, Charvet, L. Claude, Resanović, Radmila, Trailović, Saša, "Ascaridia galli infection in laying hens and the results of in vitro efficacy of levamisole, piperazine and carvacrol, whether is necessary to change the deworming protocols?" in Acta Veterinaria-Beograd, 69, no. 4 (2019):414-425,
https://doi.org/10.2478/acve-2019-0035 . .

TY  - JOUR
AU  - Choudhary, Shivani
AU  - Marjanović, Đorđe
AU  - Wong, Colin R.
AU  - Zhang, Xiaoyu
AU  - Abongwa, Melanie
AU  - Coats, Joel R.
AU  - Trailović, Saša
AU  - Martin, Richard J.
AU  - Robertson, Alan P.
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1730
AB  - The ongoing and widespread emergence of resistance to the existing anti-nematodal pharmacopeia has made it imperative to develop new anthelminthic agents. Historically, plants have been important sources of therapeutic compounds and offer an alternative to synthetic drugs. Monoterpenoids are phytochemicals that have been shown to produce acute toxic effects in insects and nematodes. Previous studies have shown nicotinic acetylcholine receptors (nAChRs) to be possible targets for naturally occurring plant metabolites such as carvacrol and carveol. In this study we examined the effects of monoterpenoid compounds on a levamisole sensitive nAChR from Oesophagostomum dentatum and a nicotine sensitive nAChR from Ascaris suum. We expressed the receptors in Xenopus laevis oocytes and used two-electrode voltage-clamp to characterize the effect of various compounds on these cys-loop receptors. At 100 mu M the majority of these compounds acted as antagonists. Interestingly, further experiments revealed that both 0.1 mu M and 10 mu M menthol potentiated acetylcholine and levamisole responses in the levamisole sensitive receptor but not the nicotine sensitive receptor. We also investigated the effects of 0.1 mu M menthol on the contractility of A. suum somatic muscle strips. Menthol produced significant potentiation of peak contractions at each concentration of acetylcholine. The positive allosteric modulatory effects of menthol in both in vivo and in vitro experiments suggests menthol as a promising candidate for combination therapy with cholinergic anthelmintics.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Journal for Parasitology-Drugs and Drug Resistance
T1  - Menthol acts as a positive allosteric modulator on nematode levamisole sensitive nicotinic acetylcholine receptors
VL  - 9
SP  - 44
EP  - 53
DO  - 10.1016/j.ijpddr.2018.12.005
ER  - 

@article{
author = "Choudhary, Shivani and Marjanović, Đorđe and Wong, Colin R. and Zhang, Xiaoyu and Abongwa, Melanie and Coats, Joel R. and Trailović, Saša and Martin, Richard J. and Robertson, Alan P.",
year = "2019",
abstract = "The ongoing and widespread emergence of resistance to the existing anti-nematodal pharmacopeia has made it imperative to develop new anthelminthic agents. Historically, plants have been important sources of therapeutic compounds and offer an alternative to synthetic drugs. Monoterpenoids are phytochemicals that have been shown to produce acute toxic effects in insects and nematodes. Previous studies have shown nicotinic acetylcholine receptors (nAChRs) to be possible targets for naturally occurring plant metabolites such as carvacrol and carveol. In this study we examined the effects of monoterpenoid compounds on a levamisole sensitive nAChR from Oesophagostomum dentatum and a nicotine sensitive nAChR from Ascaris suum. We expressed the receptors in Xenopus laevis oocytes and used two-electrode voltage-clamp to characterize the effect of various compounds on these cys-loop receptors. At 100 mu M the majority of these compounds acted as antagonists. Interestingly, further experiments revealed that both 0.1 mu M and 10 mu M menthol potentiated acetylcholine and levamisole responses in the levamisole sensitive receptor but not the nicotine sensitive receptor. We also investigated the effects of 0.1 mu M menthol on the contractility of A. suum somatic muscle strips. Menthol produced significant potentiation of peak contractions at each concentration of acetylcholine. The positive allosteric modulatory effects of menthol in both in vivo and in vitro experiments suggests menthol as a promising candidate for combination therapy with cholinergic anthelmintics.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Journal for Parasitology-Drugs and Drug Resistance",
title = "Menthol acts as a positive allosteric modulator on nematode levamisole sensitive nicotinic acetylcholine receptors",
volume = "9",
pages = "44-53",
doi = "10.1016/j.ijpddr.2018.12.005"
}

Choudhary, S., Marjanović, Đ., Wong, C. R., Zhang, X., Abongwa, M., Coats, J. R., Trailović, S., Martin, R. J.,& Robertson, A. P.. (2019). Menthol acts as a positive allosteric modulator on nematode levamisole sensitive nicotinic acetylcholine receptors. in International Journal for Parasitology-Drugs and Drug Resistance
Elsevier Sci Ltd, Oxford., 9, 44-53.
https://doi.org/10.1016/j.ijpddr.2018.12.005

Choudhary S, Marjanović Đ, Wong CR, Zhang X, Abongwa M, Coats JR, Trailović S, Martin RJ, Robertson AP. Menthol acts as a positive allosteric modulator on nematode levamisole sensitive nicotinic acetylcholine receptors. in International Journal for Parasitology-Drugs and Drug Resistance. 2019;9:44-53.
doi:10.1016/j.ijpddr.2018.12.005 .

Choudhary, Shivani, Marjanović, Đorđe, Wong, Colin R., Zhang, Xiaoyu, Abongwa, Melanie, Coats, Joel R., Trailović, Saša, Martin, Richard J., Robertson, Alan P., "Menthol acts as a positive allosteric modulator on nematode levamisole sensitive nicotinic acetylcholine receptors" in International Journal for Parasitology-Drugs and Drug Resistance, 9 (2019):44-53,
https://doi.org/10.1016/j.ijpddr.2018.12.005 . .

TY  - JOUR
AU  - Marjanović, Đorđe
AU  - Bogunović, Danica
AU  - Milovanović, Mirjana
AU  - Marinković, Darko
AU  - Zdravković, Nemanja
AU  - Magaš, Vladimir
AU  - Trailović, Saša
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1615
AB  - In the present study we tested the dose andh time dependence of the antinematodal effects of carvacrol and tyhmol on Caenorabditis elegans, and the efficacy of carvacrol, thymol, p-cymene and cinnamaldehyde,which were administrated in the drinking water of rats naturally infected with the pinworm Syphada muris. The control treatment of the infected rats was carried out with piperazine. Thymol caused a dose and time-dependent mortality in adult C. elegans. The value of the Median Lethal Concentration (LC50) of thymol was 117.9nM after 24h and 62.89 nM after 48h of exposure. Carvacrol exhibited a higher antinematodal efficiency than thymol. The LC50 of carvacrol, after 24 hours of exposure, was 53.03 nM, while after 48 hours it was 33.83 nM. On the other hand, piperazine showed an extremely high efficacy against S. muris infection in rats. Piperazine, at a dose of 625 mg/kg bw, administered in drinking water continuously for 10 days, eliminates the infection completely. However, none of the investigated active ingredients of essential oils were effective against S. muris. The reason for the lack of efficiency may be due to their pharmacokinetic properties. A relatively low amount of, orally administered, active ingredients of essential oils reaches the distal segments of the gastrointestinal tract, where S. muris inhabits the gut (colon and cecum). The obtained results, on C. elegans, indicate a clear dose and time-dependent antinematodal effect of thymol and carvacrol. However, for clinical application, it is necessary to examine the efficacy of microencapsulated formulations with a controlled release of active ingredients of essential oils in certain parts of the gastrointestinal tract.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Antihelminic activity of carvacrol, thymol, cinnamaldehyde and p-cymen against the free-living nematode caenorhabditis elegans and rat pinworm syphacia muris
VL  - 68
IS  - 4
SP  - 445
EP  - 456
DO  - 10.2478/acve-2018-0036
ER  - 

@article{
author = "Marjanović, Đorđe and Bogunović, Danica and Milovanović, Mirjana and Marinković, Darko and Zdravković, Nemanja and Magaš, Vladimir and Trailović, Saša",
year = "2018",
abstract = "In the present study we tested the dose andh time dependence of the antinematodal effects of carvacrol and tyhmol on Caenorabditis elegans, and the efficacy of carvacrol, thymol, p-cymene and cinnamaldehyde,which were administrated in the drinking water of rats naturally infected with the pinworm Syphada muris. The control treatment of the infected rats was carried out with piperazine. Thymol caused a dose and time-dependent mortality in adult C. elegans. The value of the Median Lethal Concentration (LC50) of thymol was 117.9nM after 24h and 62.89 nM after 48h of exposure. Carvacrol exhibited a higher antinematodal efficiency than thymol. The LC50 of carvacrol, after 24 hours of exposure, was 53.03 nM, while after 48 hours it was 33.83 nM. On the other hand, piperazine showed an extremely high efficacy against S. muris infection in rats. Piperazine, at a dose of 625 mg/kg bw, administered in drinking water continuously for 10 days, eliminates the infection completely. However, none of the investigated active ingredients of essential oils were effective against S. muris. The reason for the lack of efficiency may be due to their pharmacokinetic properties. A relatively low amount of, orally administered, active ingredients of essential oils reaches the distal segments of the gastrointestinal tract, where S. muris inhabits the gut (colon and cecum). The obtained results, on C. elegans, indicate a clear dose and time-dependent antinematodal effect of thymol and carvacrol. However, for clinical application, it is necessary to examine the efficacy of microencapsulated formulations with a controlled release of active ingredients of essential oils in certain parts of the gastrointestinal tract.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Antihelminic activity of carvacrol, thymol, cinnamaldehyde and p-cymen against the free-living nematode caenorhabditis elegans and rat pinworm syphacia muris",
volume = "68",
number = "4",
pages = "445-456",
doi = "10.2478/acve-2018-0036"
}

Marjanović, Đ., Bogunović, D., Milovanović, M., Marinković, D., Zdravković, N., Magaš, V.,& Trailović, S.. (2018). Antihelminic activity of carvacrol, thymol, cinnamaldehyde and p-cymen against the free-living nematode caenorhabditis elegans and rat pinworm syphacia muris. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(4), 445-456.
https://doi.org/10.2478/acve-2018-0036

Marjanović Đ, Bogunović D, Milovanović M, Marinković D, Zdravković N, Magaš V, Trailović S. Antihelminic activity of carvacrol, thymol, cinnamaldehyde and p-cymen against the free-living nematode caenorhabditis elegans and rat pinworm syphacia muris. in Acta Veterinaria-Beograd. 2018;68(4):445-456.
doi:10.2478/acve-2018-0036 .

Marjanović, Đorđe, Bogunović, Danica, Milovanović, Mirjana, Marinković, Darko, Zdravković, Nemanja, Magaš, Vladimir, Trailović, Saša, "Antihelminic activity of carvacrol, thymol, cinnamaldehyde and p-cymen against the free-living nematode caenorhabditis elegans and rat pinworm syphacia muris" in Acta Veterinaria-Beograd, 68, no. 4 (2018):445-456,
https://doi.org/10.2478/acve-2018-0036 . .

TY  - JOUR
AU  - Abongwa, Melanie
AU  - Marjanović, Đorđe
AU  - Tipton, James G.
AU  - Zheng, Fudan
AU  - Martin, Richard J.
AU  - Trailović, Saša
AU  - Robertson, Alan P.
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1622
AB  - Zolvix (R) is a recently introduced anthelmintic drench containing monepantel as the active ingredient. Monepantel is a positive allosteric modulator of DEG-3/DES-2 type nicotinic acetylcholine receptors (nAChRs) in several nematode species. The drug has been reported to produce hypercontraction of Caenorhabditis elegans and Haemonchus contortus somatic muscle. We investigated the effects of monepantel on nAChRs from Ascaris suum and Oesophagostomum dentatum heterologously expressed in Xenopus laevis oocytes. Using two-electrode voltagec-lamp electrophysiology, we studied the effects of monepantel on a nicotine preferring homomeric nAChR subtype from A. suum comprising of ACR-16; a pyrantel/tribendimidine preferring heteromeric subtype from O. dentatum comprising UNC-29, UNC-38 and UNC-63 subunits; and a levamisole preferring subtype (O. dentatum) comprising UNC-29, UNC-38, UNC-63 and ACR-8 subunits. For each subtype tested, monepantel applied in isolation produced no measurable currents thereby ruling out an agonist action. When monepantel was continuously applied, it reduced the amplitude of acetylcholine induced currents in a concentration-dependent manner. In all three subtypes, monepantel acted as a non-competitive antagonist on the expressed receptors. ACR-16 from A. suum was particularly sensitive to monepantel inhibition (IC50 values: 1.6 +/- 3.1 nM and 0.2 +/- 2.3 mu M). We also investigated the effects of monepantel on muscle flaps isolated from adult A. suum. The drug did not significantly increase baseline tension when applied on its own. As with acetylcholine induced currents in the heterologously expressed receptors, contractions induced by acetylcholine were antagonized by monepantel. Further investigation revealed that the inhibition was a mixture of competitive and non-competitive antagonism. Our findings suggest that monepantel is active on multiple nAChR subtypes.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Journal for Parasitology-Drugs and Drug Resistance
T1  - Monepantel is a non-competitive antagonist of nicotinic acetylcholine receptors from Ascaris suum and Oesophagostomum dentatum
VL  - 8
IS  - 1
SP  - 36
EP  - 42
DO  - 10.1016/j.ijpddr.2017.12.001
ER  - 

@article{
author = "Abongwa, Melanie and Marjanović, Đorđe and Tipton, James G. and Zheng, Fudan and Martin, Richard J. and Trailović, Saša and Robertson, Alan P.",
year = "2018",
abstract = "Zolvix (R) is a recently introduced anthelmintic drench containing monepantel as the active ingredient. Monepantel is a positive allosteric modulator of DEG-3/DES-2 type nicotinic acetylcholine receptors (nAChRs) in several nematode species. The drug has been reported to produce hypercontraction of Caenorhabditis elegans and Haemonchus contortus somatic muscle. We investigated the effects of monepantel on nAChRs from Ascaris suum and Oesophagostomum dentatum heterologously expressed in Xenopus laevis oocytes. Using two-electrode voltagec-lamp electrophysiology, we studied the effects of monepantel on a nicotine preferring homomeric nAChR subtype from A. suum comprising of ACR-16; a pyrantel/tribendimidine preferring heteromeric subtype from O. dentatum comprising UNC-29, UNC-38 and UNC-63 subunits; and a levamisole preferring subtype (O. dentatum) comprising UNC-29, UNC-38, UNC-63 and ACR-8 subunits. For each subtype tested, monepantel applied in isolation produced no measurable currents thereby ruling out an agonist action. When monepantel was continuously applied, it reduced the amplitude of acetylcholine induced currents in a concentration-dependent manner. In all three subtypes, monepantel acted as a non-competitive antagonist on the expressed receptors. ACR-16 from A. suum was particularly sensitive to monepantel inhibition (IC50 values: 1.6 +/- 3.1 nM and 0.2 +/- 2.3 mu M). We also investigated the effects of monepantel on muscle flaps isolated from adult A. suum. The drug did not significantly increase baseline tension when applied on its own. As with acetylcholine induced currents in the heterologously expressed receptors, contractions induced by acetylcholine were antagonized by monepantel. Further investigation revealed that the inhibition was a mixture of competitive and non-competitive antagonism. Our findings suggest that monepantel is active on multiple nAChR subtypes.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Journal for Parasitology-Drugs and Drug Resistance",
title = "Monepantel is a non-competitive antagonist of nicotinic acetylcholine receptors from Ascaris suum and Oesophagostomum dentatum",
volume = "8",
number = "1",
pages = "36-42",
doi = "10.1016/j.ijpddr.2017.12.001"
}

Abongwa, M., Marjanović, Đ., Tipton, J. G., Zheng, F., Martin, R. J., Trailović, S.,& Robertson, A. P.. (2018). Monepantel is a non-competitive antagonist of nicotinic acetylcholine receptors from Ascaris suum and Oesophagostomum dentatum. in International Journal for Parasitology-Drugs and Drug Resistance
Elsevier Sci Ltd, Oxford., 8(1), 36-42.
https://doi.org/10.1016/j.ijpddr.2017.12.001

Abongwa M, Marjanović Đ, Tipton JG, Zheng F, Martin RJ, Trailović S, Robertson AP. Monepantel is a non-competitive antagonist of nicotinic acetylcholine receptors from Ascaris suum and Oesophagostomum dentatum. in International Journal for Parasitology-Drugs and Drug Resistance. 2018;8(1):36-42.
doi:10.1016/j.ijpddr.2017.12.001 .

Abongwa, Melanie, Marjanović, Đorđe, Tipton, James G., Zheng, Fudan, Martin, Richard J., Trailović, Saša, Robertson, Alan P., "Monepantel is a non-competitive antagonist of nicotinic acetylcholine receptors from Ascaris suum and Oesophagostomum dentatum" in International Journal for Parasitology-Drugs and Drug Resistance, 8, no. 1 (2018):36-42,
https://doi.org/10.1016/j.ijpddr.2017.12.001 . .