TY  - JOUR
AU  - Dmitric, Marko
AU  - Vidanovic, Dejan
AU  - Matovic, Kazimir
AU  - Tesovic, Bojana
AU  - Sekler, Milanko
AU  - Vićić, Ivan
AU  - Karabasil, Nedjeljko
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3234
AB  - In this study, the primers and the probe were designed, and a completely new real-time polymerase chain reaction (PCR) protocol for detecting the invA gene of Salmonella in food was optimised and validated in-house. The inclusivity test used 76 different Salmonella isolates with no false-negative results. The exclusivity was tested using 45 non-Salmonella microorganisms with no false-positive results. The method was also successfully applied while examining five different artificially contaminated food categories. The results were compared to the standard method (ISO 6579-1) and two previously validated real-time PCR methods. The developed assay is sensitive and specific for rapidly detecting Salmonella in food.
PB  - Czech Academy of Agricultural Sciences
T2  - Czech Journal of Food Sciences
T1  - Development of a novel invA gene-based real-time PCR assay for the detection of Salmonella in food
VL  - 41
IS  - 4
SP  - 287
EP  - 294
DO  - 10.17221/114/2022-CJFS
ER  - 

@article{
author = "Dmitric, Marko and Vidanovic, Dejan and Matovic, Kazimir and Tesovic, Bojana and Sekler, Milanko and Vićić, Ivan and Karabasil, Nedjeljko",
year = "2023",
abstract = "In this study, the primers and the probe were designed, and a completely new real-time polymerase chain reaction (PCR) protocol for detecting the invA gene of Salmonella in food was optimised and validated in-house. The inclusivity test used 76 different Salmonella isolates with no false-negative results. The exclusivity was tested using 45 non-Salmonella microorganisms with no false-positive results. The method was also successfully applied while examining five different artificially contaminated food categories. The results were compared to the standard method (ISO 6579-1) and two previously validated real-time PCR methods. The developed assay is sensitive and specific for rapidly detecting Salmonella in food.",
publisher = "Czech Academy of Agricultural Sciences",
journal = "Czech Journal of Food Sciences",
title = "Development of a novel invA gene-based real-time PCR assay for the detection of Salmonella in food",
volume = "41",
number = "4",
pages = "287-294",
doi = "10.17221/114/2022-CJFS"
}

Dmitric, M., Vidanovic, D., Matovic, K., Tesovic, B., Sekler, M., Vićić, I.,& Karabasil, N.. (2023). Development of a novel invA gene-based real-time PCR assay for the detection of Salmonella in food. in Czech Journal of Food Sciences
Czech Academy of Agricultural Sciences., 41(4), 287-294.
https://doi.org/10.17221/114/2022-CJFS

Dmitric M, Vidanovic D, Matovic K, Tesovic B, Sekler M, Vićić I, Karabasil N. Development of a novel invA gene-based real-time PCR assay for the detection of Salmonella in food. in Czech Journal of Food Sciences. 2023;41(4):287-294.
doi:10.17221/114/2022-CJFS .

Dmitric, Marko, Vidanovic, Dejan, Matovic, Kazimir, Tesovic, Bojana, Sekler, Milanko, Vićić, Ivan, Karabasil, Nedjeljko, "Development of a novel invA gene-based real-time PCR assay for the detection of Salmonella in food" in Czech Journal of Food Sciences, 41, no. 4 (2023):287-294,
https://doi.org/10.17221/114/2022-CJFS . .