TY  - JOUR
AU  - Pirković, Andrea
AU  - Vilotić, Aleksandra
AU  - Borozan, Sunčica
AU  - Nacka-Aleksić, Mirjana
AU  - Bojić-Trbojević, Žanka
AU  - Jovanović-Krivokuća, Milica
AU  - Battino, Maurizio
AU  - Giampieri, Francesca
AU  - Dekanski, Dragana
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2539
AB  - Olive-derived bioactive compound oleuropein was evaluated against damage induced by hydrogen peroxide in human trophoblast cells in vitro, by examining the changes in several markers implicated in oxidative stress interactions in the placenta. Trophoblast HTR-8/SVneo cells were preincubated with OLE at 10 and 100 µM and exposed to H2O2, as a model of oxidative stress. Protein and lipid peroxidation, as well as antioxidant enzymes’ activity, were determined spectrophotometrically, and DNA damage was evaluated by comet assay. iNOS protein expression was assessed by Western blot, while the mRNA expression of pro- and anti-apoptotic genes BAX and BCL2 and transcription factor NFE2L2, as well as cytokines IL-6 and TNF α were determined by qPCR. Oleuropein demonstrated cytoprotective effects against H2O2 in trophoblast cells by significantly improving the antioxidant status and preventing protein and lipid damage, as well as reducing the iNOS levels. OLE reduced the mRNA expression of IL-6 and TNF α, however, it did not influence the expression of NFE2L2 or the BAX/BCL2 ratio after H2O2 exposure. Oleuropein per se did not lead to any adverse effects in HTR-8/SVneo cells under the described conditions, confirming its safety in vitro. In conclusion, it significantly attenuated oxidative damage and restored antioxidant functioning, confirming its protective role in trophoblast. © 2023 by the authors.
PB  - MDPI
T2  - Antioxidants
T1  - Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells
VL  - 12
VL  - 1
SP  - 197
DO  - 10.3390/antiox12010197
ER  - 

@article{
author = "Pirković, Andrea and Vilotić, Aleksandra and Borozan, Sunčica and Nacka-Aleksić, Mirjana and Bojić-Trbojević, Žanka and Jovanović-Krivokuća, Milica and Battino, Maurizio and Giampieri, Francesca and Dekanski, Dragana",
year = "2023",
abstract = "Olive-derived bioactive compound oleuropein was evaluated against damage induced by hydrogen peroxide in human trophoblast cells in vitro, by examining the changes in several markers implicated in oxidative stress interactions in the placenta. Trophoblast HTR-8/SVneo cells were preincubated with OLE at 10 and 100 µM and exposed to H2O2, as a model of oxidative stress. Protein and lipid peroxidation, as well as antioxidant enzymes’ activity, were determined spectrophotometrically, and DNA damage was evaluated by comet assay. iNOS protein expression was assessed by Western blot, while the mRNA expression of pro- and anti-apoptotic genes BAX and BCL2 and transcription factor NFE2L2, as well as cytokines IL-6 and TNF α were determined by qPCR. Oleuropein demonstrated cytoprotective effects against H2O2 in trophoblast cells by significantly improving the antioxidant status and preventing protein and lipid damage, as well as reducing the iNOS levels. OLE reduced the mRNA expression of IL-6 and TNF α, however, it did not influence the expression of NFE2L2 or the BAX/BCL2 ratio after H2O2 exposure. Oleuropein per se did not lead to any adverse effects in HTR-8/SVneo cells under the described conditions, confirming its safety in vitro. In conclusion, it significantly attenuated oxidative damage and restored antioxidant functioning, confirming its protective role in trophoblast. © 2023 by the authors.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells",
volume = "12, 1",
pages = "197",
doi = "10.3390/antiox12010197"
}

Pirković, A., Vilotić, A., Borozan, S., Nacka-Aleksić, M., Bojić-Trbojević, Ž., Jovanović-Krivokuća, M., Battino, M., Giampieri, F.,& Dekanski, D.. (2023). Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells. in Antioxidants
MDPI., 12, 197.
https://doi.org/10.3390/antiox12010197

Pirković A, Vilotić A, Borozan S, Nacka-Aleksić M, Bojić-Trbojević Ž, Jovanović-Krivokuća M, Battino M, Giampieri F, Dekanski D. Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells. in Antioxidants. 2023;12:197.
doi:10.3390/antiox12010197 .

Pirković, Andrea, Vilotić, Aleksandra, Borozan, Sunčica, Nacka-Aleksić, Mirjana, Bojić-Trbojević, Žanka, Jovanović-Krivokuća, Milica, Battino, Maurizio, Giampieri, Francesca, Dekanski, Dragana, "Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells" in Antioxidants, 12 (2023):197,
https://doi.org/10.3390/antiox12010197 . .

TY  - JOUR
AU  - Kostić, Sanja
AU  - Vilotić, Aleksandra
AU  - Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Borozan, Sunčica
AU  - Nacka-Aleksić, Mirjana
AU  - Vrzić-Petronijević, Svetlana
AU  - Jovanović Krivokuća, Milica
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2842
AB  - Caffeic acid is highlighted as one of the major phenolic compounds present in foods with known antioxidant activity. This phenolic is among commonly consumed substances in everyday diet of pregnant women. However, there is not enough information on its effects during pregnancy, especially the most vulnerable early stage. Extravillous trophoblast cells are specific cells of the placenta that come in direct contact with maternal uterine tissue. Through this study we investigated the cytoprotective effects of caffeic acid on H2O2-induced oxidative damage in first trimester extravillous trophoblast cell line HTR-8/SVneo. Investigated concentrations (1–100 μM) of caffeic acid showed neither cytotoxic nor genotoxic effects on HTR-8/SVneo cells. The treatment with caffeic acid 100 μM significantly increased the percentage of cells in G2/M phase of the cell cycle, compared to non-treated cells. Pretreatment with caffeic acid (10 and 100 μM) attenuated oxidative DNA damage significantly, reduced cytotoxicity, protein and lipid peroxidation, and restored antioxidant capacity in trophoblast cells following H2O2 exposure. This beneficial outcome is probably mediated by the augmentation of GSH and effective ROS scavenging by caffeic acid. These promising results require further investigations to reveal the additional mechanisms/pathways and confirmation through studies in vivo.
PB  - Elsevier
T2  - Food and Chemical Toxicology
T1  - Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity
VL  - 163
SP  - 112993
DO  - 10.1016/j.fct.2022.112993
ER  - 

@article{
author = "Kostić, Sanja and Vilotić, Aleksandra and Pirković, Andrea and Dekanski, Dragana and Borozan, Sunčica and Nacka-Aleksić, Mirjana and Vrzić-Petronijević, Svetlana and Jovanović Krivokuća, Milica",
year = "2022",
abstract = "Caffeic acid is highlighted as one of the major phenolic compounds present in foods with known antioxidant activity. This phenolic is among commonly consumed substances in everyday diet of pregnant women. However, there is not enough information on its effects during pregnancy, especially the most vulnerable early stage. Extravillous trophoblast cells are specific cells of the placenta that come in direct contact with maternal uterine tissue. Through this study we investigated the cytoprotective effects of caffeic acid on H2O2-induced oxidative damage in first trimester extravillous trophoblast cell line HTR-8/SVneo. Investigated concentrations (1–100 μM) of caffeic acid showed neither cytotoxic nor genotoxic effects on HTR-8/SVneo cells. The treatment with caffeic acid 100 μM significantly increased the percentage of cells in G2/M phase of the cell cycle, compared to non-treated cells. Pretreatment with caffeic acid (10 and 100 μM) attenuated oxidative DNA damage significantly, reduced cytotoxicity, protein and lipid peroxidation, and restored antioxidant capacity in trophoblast cells following H2O2 exposure. This beneficial outcome is probably mediated by the augmentation of GSH and effective ROS scavenging by caffeic acid. These promising results require further investigations to reveal the additional mechanisms/pathways and confirmation through studies in vivo.",
publisher = "Elsevier",
journal = "Food and Chemical Toxicology",
title = "Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity",
volume = "163",
pages = "112993",
doi = "10.1016/j.fct.2022.112993"
}

Kostić, S., Vilotić, A., Pirković, A., Dekanski, D., Borozan, S., Nacka-Aleksić, M., Vrzić-Petronijević, S.,& Jovanović Krivokuća, M.. (2022). Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity. in Food and Chemical Toxicology
Elsevier., 163, 112993.
https://doi.org/10.1016/j.fct.2022.112993

Kostić S, Vilotić A, Pirković A, Dekanski D, Borozan S, Nacka-Aleksić M, Vrzić-Petronijević S, Jovanović Krivokuća M. Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity. in Food and Chemical Toxicology. 2022;163:112993.
doi:10.1016/j.fct.2022.112993 .

Kostić, Sanja, Vilotić, Aleksandra, Pirković, Andrea, Dekanski, Dragana, Borozan, Sunčica, Nacka-Aleksić, Mirjana, Vrzić-Petronijević, Svetlana, Jovanović Krivokuća, Milica, "Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity" in Food and Chemical Toxicology, 163 (2022):112993,
https://doi.org/10.1016/j.fct.2022.112993 . .