TY  - JOUR
AU  - Bruić, Marija
AU  - Pirković, Andrea
AU  - Borozan, Sunčica
AU  - Nacka Aleksić, Mirjana
AU  - Jovanović Krivokuća, Milica
AU  - Spremo-Potparević, Biljana
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3864
AB  - Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant secondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing
these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of
oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory
cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 µM and 100 µM
concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content,
extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant enzymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along
with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed antiinflammatory activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and antiinflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing potential as a therapeutic agent in preventing adverse pregnancy outcomes.
PB  - Elsevier
T2  - Reproductive Toxicology
T1  - Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line
VL  - 126
SP  - 108585
DO  - 10.1016/j.reprotox.2024.108585
ER  - 

@article{
author = "Bruić, Marija and Pirković, Andrea and Borozan, Sunčica and Nacka Aleksić, Mirjana and Jovanović Krivokuća, Milica and Spremo-Potparević, Biljana",
year = "2024",
abstract = "Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant secondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing
these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of
oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory
cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 µM and 100 µM
concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content,
extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant enzymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along
with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed antiinflammatory activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and antiinflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing potential as a therapeutic agent in preventing adverse pregnancy outcomes.",
publisher = "Elsevier",
journal = "Reproductive Toxicology",
title = "Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line",
volume = "126",
pages = "108585",
doi = "10.1016/j.reprotox.2024.108585"
}

Bruić, M., Pirković, A., Borozan, S., Nacka Aleksić, M., Jovanović Krivokuća, M.,& Spremo-Potparević, B.. (2024). Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line. in Reproductive Toxicology
Elsevier., 126, 108585.
https://doi.org/10.1016/j.reprotox.2024.108585

Bruić M, Pirković A, Borozan S, Nacka Aleksić M, Jovanović Krivokuća M, Spremo-Potparević B. Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line. in Reproductive Toxicology. 2024;126:108585.
doi:10.1016/j.reprotox.2024.108585 .

Bruić, Marija, Pirković, Andrea, Borozan, Sunčica, Nacka Aleksić, Mirjana, Jovanović Krivokuća, Milica, Spremo-Potparević, Biljana, "Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line" in Reproductive Toxicology, 126 (2024):108585,
https://doi.org/10.1016/j.reprotox.2024.108585 . .

TY  - CONF
AU  - Radaković, Milena
AU  - Borozan, Sunčica
AU  - Đelić, Ninoslav
AU  - Ivanović, Saša
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Stanimirović, Zoran
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2717
AB  - The catecholamines have always attracted interest for investigation due to physiological
and pathological effects. This study aims to point on biochemical endpoints of toxic
effects after administration of adrenaline. For this purpose the study was carried out on
Wistar rats and three experimental doses of adrenaline were used: 0.75 mg/kg, 1.5
mg/kg and 3 mg/kg.
We investigated the effects of adrenaline on total activity of lactate dehydrogenase
(LDH), carbonyl groups using spectrophotometric methods, concentrations of nitrite by
ELISA test and nitrotyrosine by Western blotting and SDS-PAGE. The level of acute
phase proteins (APPs) on alkaline-PAGE, alpha-1-acid glycoprotein and haptoglobulin
by Western blot and SDS-PAGE treated with adrenaline was also determined. The
obtained results revealed that all doses of adrenaline induced significant rise in total
activity of LDH and concentrations of carbonyl groups compared to control.
Accordingly, adrenaline exerted significant increase in concentration of nitrite and
nitrotyrosine derivate in a dose dependent manner. Further study indicated that
adrenaline significantly decreased serum albumine level and albumin-globulin ratio,
while the APPs level is increased (alpha-1-acid glycoprotein and haptoglobulin). Based
on the results it can be concluded that adrenaline causes significant alterations in APPs
and increased protein oxidative damage, which may contribute to better understanding
its toxic effects.
C3  - 3rd International Conference of Environmental and Occupational Health, Porto, 21-23 June 2016
T1  - Protein oxidative damage and level of acute phase proteins in wistar rats treated with adrenaline
IS  - 71
EP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2717
ER  - 

@conference{
author = "Radaković, Milena and Borozan, Sunčica and Đelić, Ninoslav and Ivanović, Saša and Spremo-Potparević, Biljana and Živković, Lada and Stanimirović, Zoran",
year = "2016",
abstract = "The catecholamines have always attracted interest for investigation due to physiological
and pathological effects. This study aims to point on biochemical endpoints of toxic
effects after administration of adrenaline. For this purpose the study was carried out on
Wistar rats and three experimental doses of adrenaline were used: 0.75 mg/kg, 1.5
mg/kg and 3 mg/kg.
We investigated the effects of adrenaline on total activity of lactate dehydrogenase
(LDH), carbonyl groups using spectrophotometric methods, concentrations of nitrite by
ELISA test and nitrotyrosine by Western blotting and SDS-PAGE. The level of acute
phase proteins (APPs) on alkaline-PAGE, alpha-1-acid glycoprotein and haptoglobulin
by Western blot and SDS-PAGE treated with adrenaline was also determined. The
obtained results revealed that all doses of adrenaline induced significant rise in total
activity of LDH and concentrations of carbonyl groups compared to control.
Accordingly, adrenaline exerted significant increase in concentration of nitrite and
nitrotyrosine derivate in a dose dependent manner. Further study indicated that
adrenaline significantly decreased serum albumine level and albumin-globulin ratio,
while the APPs level is increased (alpha-1-acid glycoprotein and haptoglobulin). Based
on the results it can be concluded that adrenaline causes significant alterations in APPs
and increased protein oxidative damage, which may contribute to better understanding
its toxic effects.",
journal = "3rd International Conference of Environmental and Occupational Health, Porto, 21-23 June 2016",
title = "Protein oxidative damage and level of acute phase proteins in wistar rats treated with adrenaline",
number = "71",
pages = "71",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2717"
}

Radaković, M., Borozan, S., Đelić, N., Ivanović, S., Spremo-Potparević, B., Živković, L.,& Stanimirović, Z.. (2016). Protein oxidative damage and level of acute phase proteins in wistar rats treated with adrenaline. in 3rd International Conference of Environmental and Occupational Health, Porto, 21-23 June 2016(71).
https://hdl.handle.net/21.15107/rcub_veterinar_2717

Radaković M, Borozan S, Đelić N, Ivanović S, Spremo-Potparević B, Živković L, Stanimirović Z. Protein oxidative damage and level of acute phase proteins in wistar rats treated with adrenaline. in 3rd International Conference of Environmental and Occupational Health, Porto, 21-23 June 2016. 2016;(71):null-71.
https://hdl.handle.net/21.15107/rcub_veterinar_2717 .

Radaković, Milena, Borozan, Sunčica, Đelić, Ninoslav, Ivanović, Saša, Spremo-Potparević, Biljana, Živković, Lada, Stanimirović, Zoran, "Protein oxidative damage and level of acute phase proteins in wistar rats treated with adrenaline" in 3rd International Conference of Environmental and Occupational Health, Porto, 21-23 June 2016, no. 71 (2016),
https://hdl.handle.net/21.15107/rcub_veterinar_2717 .

TY  - JOUR
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2812
AB  - Premature centromere division (PCD) of the chromosome 18 was analyzed by using fluorescent in situ hybridization (FISH) on interphase peripheral blood lymphocytes isolated from six sporadic Alzheimer disease (AD) patients and six healthy elderly controls. Results of FISH analysis revealed that chromosome 18 expressed PCD in 5.18% interphase nuclei of AD patients, and in 2.59% interphase nuclei of age-matched controls (p < 0.05). Our study also showed that hypoploidy and hyperploidy frequency for chromosome 18 exhibited a statistically significant increase in the AD group compared to the control one. The increase in spontaneous aneuploidy of chromosome 18 in AD patients which is correlated with PCD shows that deregulation of the time of centromere separation can be considered as a manifestation of chromosome instability leading to aneuploidy.
PB  - Elsevier
T2  - Mechanisms of Ageing and Development
T1  - Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes of Alzheimer disease patients
VL  - 127
IS  - 12
SP  - 892
EP  - 896
DO  - 10.1016/j.mad.2006.09.004
ER  - 

@article{
author = "Živković, Lada and Spremo-Potparević, Biljana and Đelić, Ninoslav and Bajić, Vladan",
year = "2006",
abstract = "Premature centromere division (PCD) of the chromosome 18 was analyzed by using fluorescent in situ hybridization (FISH) on interphase peripheral blood lymphocytes isolated from six sporadic Alzheimer disease (AD) patients and six healthy elderly controls. Results of FISH analysis revealed that chromosome 18 expressed PCD in 5.18% interphase nuclei of AD patients, and in 2.59% interphase nuclei of age-matched controls (p < 0.05). Our study also showed that hypoploidy and hyperploidy frequency for chromosome 18 exhibited a statistically significant increase in the AD group compared to the control one. The increase in spontaneous aneuploidy of chromosome 18 in AD patients which is correlated with PCD shows that deregulation of the time of centromere separation can be considered as a manifestation of chromosome instability leading to aneuploidy.",
publisher = "Elsevier",
journal = "Mechanisms of Ageing and Development",
title = "Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes of Alzheimer disease patients",
volume = "127",
number = "12",
pages = "892-896",
doi = "10.1016/j.mad.2006.09.004"
}

Živković, L., Spremo-Potparević, B., Đelić, N.,& Bajić, V.. (2006). Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes of Alzheimer disease patients. in Mechanisms of Ageing and Development
Elsevier., 127(12), 892-896.
https://doi.org/10.1016/j.mad.2006.09.004

Živković L, Spremo-Potparević B, Đelić N, Bajić V. Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes of Alzheimer disease patients. in Mechanisms of Ageing and Development. 2006;127(12):892-896.
doi:10.1016/j.mad.2006.09.004 .

Živković, Lada, Spremo-Potparević, Biljana, Đelić, Ninoslav, Bajić, Vladan, "Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes of Alzheimer disease patients" in Mechanisms of Ageing and Development, 127, no. 12 (2006):892-896,
https://doi.org/10.1016/j.mad.2006.09.004 . .