TY  - JOUR
AU  - Vranješ, Borjana
AU  - Milićević, Dragan
AU  - Šefer, Dragan
AU  - Stefanović, Srđan
AU  - Ajtić, Jelena
AU  - Mitrović, Branislava
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1809
AB  - Naturally occurring radionuclides and toxic elements are ubiquitous in the environment and can pose a great risk to animals health. Control of these toxic substances in animal diet is essential for animals welfare, human nutrition risk management as well as environmental protection. Our study addresses these issues by determining a content of natural radionuclides (K-40, U-238 and Ra-226), and toxic elements (Cd, Cr, As) in total of 108 samples: 36 monocalcium phosphate, 36 complete feed and 36 pig manure samples that were collected from 6 different commercial farms in the Republic of Serbia. In monocalcium phosphate the content of K-40, U-238 and Ra-226 ranged from 7.6-23.4 Bq kg(-1), 13.2-1778 Bq kg(-1) and 3.6-13.5 Bq kg(-)1, respectively. In complete feed samples, 40K ranged from 170 to 306 Bq kg(-1), U-238 from minimum detectable activity (MDA) to 9.1 Bq kg(-1), and Ra-226 from MDA-5.5 Bq kg(-1), while in pig manure K-40 ranged from 146 to 531 Bq kg(-1), U-238 from MDA-30.1 Bq kg(-1), and Ra-226 from MDA-10 Bq kg(-1). The concentrations of Cd, Cr and As in monocalcium phosphate were 0.26-14.9 mg kg(-1), 10.3-497 mg kg(-1), and 4.2-5.5 mg kg(-1), respectively. In complete feed, the content of Cd, Cr and As ranged from minimum detectable concentration (MDC) - 0.78 mg kg-1, 0.30-7.41 mg kg(-1), and 0.09-0.31 mg kg(-1), respectively. In pig manure Cd, Cr and As ranged from 0.8-2.3 mg kg(-1), 2.3-22.2 mg kg(-1), and 0.51-5.45 mg kg(-1), respectively. The obtained results showed that Cd concentration inmonocalcium phosphate exceeded the maximum permissible legislative concentrations for phosphate additives. Monocalcium phosphate is the main source of U-238 and Cr in complete feed, while Cd derives from other sources. For that reason excessive use of monocalcium phosphate can lead to high levels of these pollutants in complete feed and consequently in manure.
PB  - Elsevier, Amsterdam
T2  - Science of The Total Environment
T1  - Presence of natural radionuclides and toxic elements in monocalcium phosphate, complete feed and pig manure
VL  - 720
SP  - 137578
DO  - 10.1016/j.scitotenv.2020.137578
ER  - 

@article{
author = "Vranješ, Borjana and Milićević, Dragan and Šefer, Dragan and Stefanović, Srđan and Ajtić, Jelena and Mitrović, Branislava",
year = "2020",
abstract = "Naturally occurring radionuclides and toxic elements are ubiquitous in the environment and can pose a great risk to animals health. Control of these toxic substances in animal diet is essential for animals welfare, human nutrition risk management as well as environmental protection. Our study addresses these issues by determining a content of natural radionuclides (K-40, U-238 and Ra-226), and toxic elements (Cd, Cr, As) in total of 108 samples: 36 monocalcium phosphate, 36 complete feed and 36 pig manure samples that were collected from 6 different commercial farms in the Republic of Serbia. In monocalcium phosphate the content of K-40, U-238 and Ra-226 ranged from 7.6-23.4 Bq kg(-1), 13.2-1778 Bq kg(-1) and 3.6-13.5 Bq kg(-)1, respectively. In complete feed samples, 40K ranged from 170 to 306 Bq kg(-1), U-238 from minimum detectable activity (MDA) to 9.1 Bq kg(-1), and Ra-226 from MDA-5.5 Bq kg(-1), while in pig manure K-40 ranged from 146 to 531 Bq kg(-1), U-238 from MDA-30.1 Bq kg(-1), and Ra-226 from MDA-10 Bq kg(-1). The concentrations of Cd, Cr and As in monocalcium phosphate were 0.26-14.9 mg kg(-1), 10.3-497 mg kg(-1), and 4.2-5.5 mg kg(-1), respectively. In complete feed, the content of Cd, Cr and As ranged from minimum detectable concentration (MDC) - 0.78 mg kg-1, 0.30-7.41 mg kg(-1), and 0.09-0.31 mg kg(-1), respectively. In pig manure Cd, Cr and As ranged from 0.8-2.3 mg kg(-1), 2.3-22.2 mg kg(-1), and 0.51-5.45 mg kg(-1), respectively. The obtained results showed that Cd concentration inmonocalcium phosphate exceeded the maximum permissible legislative concentrations for phosphate additives. Monocalcium phosphate is the main source of U-238 and Cr in complete feed, while Cd derives from other sources. For that reason excessive use of monocalcium phosphate can lead to high levels of these pollutants in complete feed and consequently in manure.",
publisher = "Elsevier, Amsterdam",
journal = "Science of The Total Environment",
title = "Presence of natural radionuclides and toxic elements in monocalcium phosphate, complete feed and pig manure",
volume = "720",
pages = "137578",
doi = "10.1016/j.scitotenv.2020.137578"
}

Vranješ, B., Milićević, D., Šefer, D., Stefanović, S., Ajtić, J.,& Mitrović, B.. (2020). Presence of natural radionuclides and toxic elements in monocalcium phosphate, complete feed and pig manure. in Science of The Total Environment
Elsevier, Amsterdam., 720, 137578.
https://doi.org/10.1016/j.scitotenv.2020.137578

Vranješ B, Milićević D, Šefer D, Stefanović S, Ajtić J, Mitrović B. Presence of natural radionuclides and toxic elements in monocalcium phosphate, complete feed and pig manure. in Science of The Total Environment. 2020;720:137578.
doi:10.1016/j.scitotenv.2020.137578 .

Vranješ, Borjana, Milićević, Dragan, Šefer, Dragan, Stefanović, Srđan, Ajtić, Jelena, Mitrović, Branislava, "Presence of natural radionuclides and toxic elements in monocalcium phosphate, complete feed and pig manure" in Science of The Total Environment, 720 (2020):137578,
https://doi.org/10.1016/j.scitotenv.2020.137578 . .

TY  - JOUR
AU  - Nikolić, Aleksandra
AU  - Đorđević, Vesna
AU  - Parunović, Nenad
AU  - Stefanović, Srđan
AU  - Đurić, Spomenka
AU  - Babić, Jelena
AU  - Vasilev, Dragan
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1865
AB  - This study was aimed at the investigation into the influence of polyphenols on fermented sausages produced with and without nitrite addition, during storage which lasted for 280 days. Three types of sausages were produced and formed the three experimental groups: C-the control-sausages of usual composition containing nitrites; N+P-sausages with nitrites and polyphenols; and P-nitrite-free sausages with added polyphenols. The proximate chemical composition of all groups was in the range with that of dry fermented sausages. P sausages contained 0.3 mg nitrites per kg, while C and N+P contained 54.8 mg/kg and 52.2 mg/kg, respectively. Polyphenol-enriched sausages had significantly lower peroxide and TBARS values than C sausages. In all sausages lactic acid bacteria counts reached 8.9-9.9 log cfu/g, but decreased during storage to 4.3-4.8 log cfu/g at the end of the storage period. Micrococcaceae counts remained stable: 3.5-3.9 log cfu/g. In P and N+P sausages a significantly lower number of Pseudomonadaceae was observed than in the control. The lightness of C and P sausages was similar (L=50.2 and L=49.5, respectively), while N+P sausages were darker (L=42.5). C and N+P sausages had similar redness (a∗=14.5 and a∗=13.2, respectively) and yellowness (b∗=5.9 and b∗=6.4, respectively), but the values which correspond to redness and yellowness were lower in P sausages (a∗=8.0 and b∗=4.6). Sensory characteristics of all products were found to be very similar. The flavour of polyphenol-enriched sausages was considered to be better. The most dominant polyphenol in sausages was kaempferol-3-O-glucoside followed by quercetin, luteolin-7-O-glucoside, catechin and syringic acid. Nitrite-free polyphenol-enriched sausages reached the same shelf life as conventional sausages containing nitrites did, which is a promising result implying that polyphenols might be used as natural preservatives and nitrite substitutes. Simultaneous use of nitrite and polyphenols is questionable due to their interactions which should be further studied.
AB  - Cilj ovog rada bio je ispitivanje uticaja polifenola na fermentisane kobasice proizvedene sa ili bez dodatka nitrita za vreme skladištenja od 280 dana. Proizvedena su tri tipa kobasica, od kojih su formirane tri eksperimentalne grupe: C - kontrolna grupa – kobasice uobičajenog sastava koje sadrže nitrite, N+P - kobasice sa nitritima i polifenolima i P - kobasice bez nitrita koje sadrže polifenole. Hemijski sastav svih grupa kobasica bio je u opsegu uobičajenom za fermentisane suve kobasice. P kobasice sadržale su 0,3 mg/kg nitrita, dok su C i N+P sadržale 54,8 mg/kg, odnosno 52,2 mg/kg. Kobasice obogaćene polifenolima imale su značajno manji peroksidni i TBARS broj nego C kobasice. Broj mlečno-kiselinskih bakterija dostigao je 8,9-9,9 log cfu/g, ali je u toku skladištenja opao na 4,3-4,8 log cfu/g posle 280dana u svim grupama kobasica. Broj Micrococcaceae bio je konstantan (3,5-3,9 log cfu/g). Kod P i N+P kobasica utvrđen je značajno niži broj Pseudomonadaceae. C i P kobasice bile su približno jednako svetle (L=50,2,odnosnoL=49,5) dok su N+P kobasice bile tamnije (L=42,5). C i N+P kobasice imale su sličan intenzitet crvne (a*=14,5 odnosno a*=13,2) i žute boje (b*=5,9 odnosno b*=6,4) ali su ove vrednosti bile niže kod P kobasica (a*=8,0 i b*=4,6). Senzorske karakteristike svih proizvoda bile su približno jednako ocenjene. Nešto bolja aroma utvrđena je od kobasica kojima su dodati polifenoli. Najdominantniji polifenol u kobasicama bio je kempferol-3-O-glukozid, a pored njega, bili su utvrđeni kvercetin, luteolin-7-O-glukozid, katehin i siringinska kiselina. Kobasice bez nitrita obogaćene polifenolima postigle su istu održivost kao i uobičajene kobasice koje sadrže nitrite, što predstavlja ohrabrujuće rezultate za mogućnost upotrebe polifenola kao prirodnog konzervansa i zamene za nitrite. Simultana upotreba nitrita i polifenola nije pouzdana zbog interakcija između polifenola i nitrita, što bi trebalo da bude detaljnije istraženo u budućnosti.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Can Polyphenols Be Used as Natural Preservatives in Fermented Sausages?
T1  - Da li polifenoli mogu da se koriste kao prirodni konzervansi u fermentisanim kobasicama?
VL  - 70
IS  - 2
SP  - 219
EP  - 237
DO  - 10.2478/acve-2020-0016
ER  - 

@article{
author = "Nikolić, Aleksandra and Đorđević, Vesna and Parunović, Nenad and Stefanović, Srđan and Đurić, Spomenka and Babić, Jelena and Vasilev, Dragan",
year = "2020",
abstract = "This study was aimed at the investigation into the influence of polyphenols on fermented sausages produced with and without nitrite addition, during storage which lasted for 280 days. Three types of sausages were produced and formed the three experimental groups: C-the control-sausages of usual composition containing nitrites; N+P-sausages with nitrites and polyphenols; and P-nitrite-free sausages with added polyphenols. The proximate chemical composition of all groups was in the range with that of dry fermented sausages. P sausages contained 0.3 mg nitrites per kg, while C and N+P contained 54.8 mg/kg and 52.2 mg/kg, respectively. Polyphenol-enriched sausages had significantly lower peroxide and TBARS values than C sausages. In all sausages lactic acid bacteria counts reached 8.9-9.9 log cfu/g, but decreased during storage to 4.3-4.8 log cfu/g at the end of the storage period. Micrococcaceae counts remained stable: 3.5-3.9 log cfu/g. In P and N+P sausages a significantly lower number of Pseudomonadaceae was observed than in the control. The lightness of C and P sausages was similar (L=50.2 and L=49.5, respectively), while N+P sausages were darker (L=42.5). C and N+P sausages had similar redness (a∗=14.5 and a∗=13.2, respectively) and yellowness (b∗=5.9 and b∗=6.4, respectively), but the values which correspond to redness and yellowness were lower in P sausages (a∗=8.0 and b∗=4.6). Sensory characteristics of all products were found to be very similar. The flavour of polyphenol-enriched sausages was considered to be better. The most dominant polyphenol in sausages was kaempferol-3-O-glucoside followed by quercetin, luteolin-7-O-glucoside, catechin and syringic acid. Nitrite-free polyphenol-enriched sausages reached the same shelf life as conventional sausages containing nitrites did, which is a promising result implying that polyphenols might be used as natural preservatives and nitrite substitutes. Simultaneous use of nitrite and polyphenols is questionable due to their interactions which should be further studied., Cilj ovog rada bio je ispitivanje uticaja polifenola na fermentisane kobasice proizvedene sa ili bez dodatka nitrita za vreme skladištenja od 280 dana. Proizvedena su tri tipa kobasica, od kojih su formirane tri eksperimentalne grupe: C - kontrolna grupa – kobasice uobičajenog sastava koje sadrže nitrite, N+P - kobasice sa nitritima i polifenolima i P - kobasice bez nitrita koje sadrže polifenole. Hemijski sastav svih grupa kobasica bio je u opsegu uobičajenom za fermentisane suve kobasice. P kobasice sadržale su 0,3 mg/kg nitrita, dok su C i N+P sadržale 54,8 mg/kg, odnosno 52,2 mg/kg. Kobasice obogaćene polifenolima imale su značajno manji peroksidni i TBARS broj nego C kobasice. Broj mlečno-kiselinskih bakterija dostigao je 8,9-9,9 log cfu/g, ali je u toku skladištenja opao na 4,3-4,8 log cfu/g posle 280dana u svim grupama kobasica. Broj Micrococcaceae bio je konstantan (3,5-3,9 log cfu/g). Kod P i N+P kobasica utvrđen je značajno niži broj Pseudomonadaceae. C i P kobasice bile su približno jednako svetle (L=50,2,odnosnoL=49,5) dok su N+P kobasice bile tamnije (L=42,5). C i N+P kobasice imale su sličan intenzitet crvne (a*=14,5 odnosno a*=13,2) i žute boje (b*=5,9 odnosno b*=6,4) ali su ove vrednosti bile niže kod P kobasica (a*=8,0 i b*=4,6). Senzorske karakteristike svih proizvoda bile su približno jednako ocenjene. Nešto bolja aroma utvrđena je od kobasica kojima su dodati polifenoli. Najdominantniji polifenol u kobasicama bio je kempferol-3-O-glukozid, a pored njega, bili su utvrđeni kvercetin, luteolin-7-O-glukozid, katehin i siringinska kiselina. Kobasice bez nitrita obogaćene polifenolima postigle su istu održivost kao i uobičajene kobasice koje sadrže nitrite, što predstavlja ohrabrujuće rezultate za mogućnost upotrebe polifenola kao prirodnog konzervansa i zamene za nitrite. Simultana upotreba nitrita i polifenola nije pouzdana zbog interakcija između polifenola i nitrita, što bi trebalo da bude detaljnije istraženo u budućnosti.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Can Polyphenols Be Used as Natural Preservatives in Fermented Sausages?, Da li polifenoli mogu da se koriste kao prirodni konzervansi u fermentisanim kobasicama?",
volume = "70",
number = "2",
pages = "219-237",
doi = "10.2478/acve-2020-0016"
}

Nikolić, A., Đorđević, V., Parunović, N., Stefanović, S., Đurić, S., Babić, J.,& Vasilev, D.. (2020). Can Polyphenols Be Used as Natural Preservatives in Fermented Sausages?. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(2), 219-237.
https://doi.org/10.2478/acve-2020-0016

Nikolić A, Đorđević V, Parunović N, Stefanović S, Đurić S, Babić J, Vasilev D. Can Polyphenols Be Used as Natural Preservatives in Fermented Sausages?. in Acta Veterinaria-Beograd. 2020;70(2):219-237.
doi:10.2478/acve-2020-0016 .

Nikolić, Aleksandra, Đorđević, Vesna, Parunović, Nenad, Stefanović, Srđan, Đurić, Spomenka, Babić, Jelena, Vasilev, Dragan, "Can Polyphenols Be Used as Natural Preservatives in Fermented Sausages?" in Acta Veterinaria-Beograd, 70, no. 2 (2020):219-237,
https://doi.org/10.2478/acve-2020-0016 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stefanović, Srđan
AU  - Karabasil, Nedjeljko
AU  - Vasilev, Dragan
AU  - Čobanović, Nikola
AU  - Ilić, Nevena
AU  - Đorđević, Vesna
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1379
AB  - The aim of this study was to determine the amount of histamine in canned fish samples collected from Belgrade retail stores using ultra-performance liquid chromatography tandem mass-spectrometry. In addition, the established levels were compared with the maximum levels set by US Food and Drug Administration (FDA) and European Union (EU) in order to assess the risk of this toxic biogenic amine to the city population. Histamine was detected in 54.07% of analyzed canned fish, in concentrations ranging from 5 to 420 mg/kg with a mean level of 60.91 mg/kg. In canned tuna, histamine levels ranged from 6 to 420 mg/kg, while in canned mackerel the concentrations ranged from 5 to 121 mg/kg. Also, the mean histamine level in canned tuna was higher than in canned mackerel (mean values were 60.91 mg/kg and 42.94 mg/kg, respectively). Among the tested canned fish, 20% of samples had higher histamine levels than the maximum level prescribed by the FDA (histamine levels >50 mg/kg), indicating definite decomposition of the fish. Histamine levels lower than 10 mg/kg were found in 51.48% of canned fish, which indicated good-quality fish products. Only 6.67% of examined production lots of canned fish had histamine levels above the regulatory limit according to the EU standard.
PB  - Institute of meat hygiene and technology, Belgrade
T2  - Meat Technology
T1  - UPLC-MS/MS determination of histamine levels in canned fish collected from Belgrade retail markets
VL  - 57
IS  - 1
SP  - 47
EP  - 56
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1379
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stefanović, Srđan and Karabasil, Nedjeljko and Vasilev, Dragan and Čobanović, Nikola and Ilić, Nevena and Đorđević, Vesna",
year = "2016",
abstract = "The aim of this study was to determine the amount of histamine in canned fish samples collected from Belgrade retail stores using ultra-performance liquid chromatography tandem mass-spectrometry. In addition, the established levels were compared with the maximum levels set by US Food and Drug Administration (FDA) and European Union (EU) in order to assess the risk of this toxic biogenic amine to the city population. Histamine was detected in 54.07% of analyzed canned fish, in concentrations ranging from 5 to 420 mg/kg with a mean level of 60.91 mg/kg. In canned tuna, histamine levels ranged from 6 to 420 mg/kg, while in canned mackerel the concentrations ranged from 5 to 121 mg/kg. Also, the mean histamine level in canned tuna was higher than in canned mackerel (mean values were 60.91 mg/kg and 42.94 mg/kg, respectively). Among the tested canned fish, 20% of samples had higher histamine levels than the maximum level prescribed by the FDA (histamine levels >50 mg/kg), indicating definite decomposition of the fish. Histamine levels lower than 10 mg/kg were found in 51.48% of canned fish, which indicated good-quality fish products. Only 6.67% of examined production lots of canned fish had histamine levels above the regulatory limit according to the EU standard.",
publisher = "Institute of meat hygiene and technology, Belgrade",
journal = "Meat Technology",
title = "UPLC-MS/MS determination of histamine levels in canned fish collected from Belgrade retail markets",
volume = "57",
number = "1",
pages = "47-56",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1379"
}

Dimitrijević, M., Stefanović, S., Karabasil, N., Vasilev, D., Čobanović, N., Ilić, N.,& Đorđević, V.. (2016). UPLC-MS/MS determination of histamine levels in canned fish collected from Belgrade retail markets. in Meat Technology
Institute of meat hygiene and technology, Belgrade., 57(1), 47-56.
https://hdl.handle.net/21.15107/rcub_veterinar_1379

Dimitrijević M, Stefanović S, Karabasil N, Vasilev D, Čobanović N, Ilić N, Đorđević V. UPLC-MS/MS determination of histamine levels in canned fish collected from Belgrade retail markets. in Meat Technology. 2016;57(1):47-56.
https://hdl.handle.net/21.15107/rcub_veterinar_1379 .

Dimitrijević, Mirjana, Stefanović, Srđan, Karabasil, Nedjeljko, Vasilev, Dragan, Čobanović, Nikola, Ilić, Nevena, Đorđević, Vesna, "UPLC-MS/MS determination of histamine levels in canned fish collected from Belgrade retail markets" in Meat Technology, 57, no. 1 (2016):47-56,
https://hdl.handle.net/21.15107/rcub_veterinar_1379 .

TY  - CONF
AU  - Stefanović, Srđan
AU  - Spirić, Danka
AU  - Petronijević, Radivoj
AU  - Nedeljković-Trailović, Jelena
AU  - Milićević, Dragan
AU  - Nikolić, Dragica M.
AU  - Janković, Saša
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1252
AB  - The aim of this paper is to assess the closeness of agreement between results of ELISA and LC-MS/MS methods for determination of aflatoxin B-1 in corn and aflatoxin M-1 in milk. Samples of corn (n=100) and milk (n=250) were simultaneously analyzed using ELISA and LC-MS/MS methods, after the severe drought that affected Serbia in summer 2012 resulting in occurrence of aflatoxin B1 in corn and aflatoxin M-1 in milk. Regression analysis showed higher level of agreement between aflatoxin B-1 samples (R2=0.994), compared to aflatoxin M-1 samples (r(2)=0.920). However, both techniques were satisfactory in meeting the requirements for official control purposes.
PB  - Elsevier Science Bv, Amsterdam
C3  - 58th International Meat Industry Conference (MEATCON2015)
T1  - Comparison of two analytical methods (ELISA and LC-MS/MS) for determination of aflatoxin B-1 in corn and aflatoxin M-1 in milk
VL  - 5
SP  - 270
EP  - 273
DO  - 10.1016/j.profoo.2015.09.077
ER  - 

@conference{
author = "Stefanović, Srđan and Spirić, Danka and Petronijević, Radivoj and Nedeljković-Trailović, Jelena and Milićević, Dragan and Nikolić, Dragica M. and Janković, Saša",
year = "2015",
abstract = "The aim of this paper is to assess the closeness of agreement between results of ELISA and LC-MS/MS methods for determination of aflatoxin B-1 in corn and aflatoxin M-1 in milk. Samples of corn (n=100) and milk (n=250) were simultaneously analyzed using ELISA and LC-MS/MS methods, after the severe drought that affected Serbia in summer 2012 resulting in occurrence of aflatoxin B1 in corn and aflatoxin M-1 in milk. Regression analysis showed higher level of agreement between aflatoxin B-1 samples (R2=0.994), compared to aflatoxin M-1 samples (r(2)=0.920). However, both techniques were satisfactory in meeting the requirements for official control purposes.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "58th International Meat Industry Conference (MEATCON2015)",
title = "Comparison of two analytical methods (ELISA and LC-MS/MS) for determination of aflatoxin B-1 in corn and aflatoxin M-1 in milk",
volume = "5",
pages = "270-273",
doi = "10.1016/j.profoo.2015.09.077"
}

Stefanović, S., Spirić, D., Petronijević, R., Nedeljković-Trailović, J., Milićević, D., Nikolić, D. M.,& Janković, S.. (2015). Comparison of two analytical methods (ELISA and LC-MS/MS) for determination of aflatoxin B-1 in corn and aflatoxin M-1 in milk. in 58th International Meat Industry Conference (MEATCON2015)
Elsevier Science Bv, Amsterdam., 5, 270-273.
https://doi.org/10.1016/j.profoo.2015.09.077

Stefanović S, Spirić D, Petronijević R, Nedeljković-Trailović J, Milićević D, Nikolić DM, Janković S. Comparison of two analytical methods (ELISA and LC-MS/MS) for determination of aflatoxin B-1 in corn and aflatoxin M-1 in milk. in 58th International Meat Industry Conference (MEATCON2015). 2015;5:270-273.
doi:10.1016/j.profoo.2015.09.077 .

Stefanović, Srđan, Spirić, Danka, Petronijević, Radivoj, Nedeljković-Trailović, Jelena, Milićević, Dragan, Nikolić, Dragica M., Janković, Saša, "Comparison of two analytical methods (ELISA and LC-MS/MS) for determination of aflatoxin B-1 in corn and aflatoxin M-1 in milk" in 58th International Meat Industry Conference (MEATCON2015), 5 (2015):270-273,
https://doi.org/10.1016/j.profoo.2015.09.077 . .

TY  - JOUR
AU  - Milanović, Svetlana
AU  - Jovanović, Ivan B.
AU  - Bjelica, Jelena
AU  - Joksimović, Srđan
AU  - Stefanović, Srđan
AU  - Radović, Magdalena
AU  - Kirovski, Danijela
AU  - Valčić, Olivera
AU  - Gvozdić, Dragan
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1121
AB  - In this work there was investigated the influence of selenium and deodinases blockers on juvenile rats body weight during three months. The experiment was carried out on 64 rats divided into eight groups with eight individual animals per group. Following groups were formed: 1. Se+PTU-IA- (control group), 2. Se+PTU+IA+, 3. Se+PTU+IA-, 4. Se+P-TU-IA+, 5. Se-PTU-IA-, 6. Se-PTU+IA+, 7. Se-PTU+IA- and 8. Se-PTU-IA+. The groups labeled (Se+) were selenium adequate and they were fed with food that contained 0.334 mg Se/kg. The groups labeled (Se-) were selenium deficient and obtained food with 0.031 mg Se/kg. As deiodinases blockers there were used propylthiouracil (PTU+) in a dose of 150 mg/L of drinking water and iopanoic acid (IA+) in a dose of 6 mg/100 g TM intraperitoneally. Body weight of experimental rats was measured every seven days. After three weeks of treatment there were taken blood samples of animals from all experimental groups and following parameters were determined: selenium concentration in blood, thyroxine (T4), triiodothyronine (T3) and thyroidstimulating hormone (TSH) in blood plasma. Analysis of the samples showed that the animals from the groups treated with PTU had lower body weight in regard to the control group, as well as lower concentration of T3 and T4 in plasma. Selenium deficient rats had lower average body weight compared to the selenium adequate ones after three weeks, but there were no differences in thyroid hormones concentration. The lowest average body weight was noticed in selenium deficient rats groups treated with PTU.
AB  - U okviru ogleda je ispitivan uticaj selena i blokatora dejodinaza na telesnu masu juvenilnih pacova tokom tri nedelje. Ogled je izveden na 64 pacova podeljenih u osam grupa od po 8 jedinki. Formirane su sledeće grupe: 1. Se+PTU-IA- (kontrolna grupa), 2. Se+PTU+IA+, 3. Se+PTU+IA-, 4. Se+PTU-IA+, 5. Se-PTU-IA-, 6. Se-PTU+IA+, 7. Se-PTU+IA- i 8. Se-PTU-IA+. Grupe sa oznakom (Se+) su bile selenadekvatne i dobijale su hranu koja je sadržala 0,334 mg Se/kg hrane. Grupe sa oznakom (Se-) su bile selendeficitne i dobijale su 0,031 mg mg Se/kg hrane. Kao blokatori dejodinaza su korišćeni propiltiouracil (PTU+) u dozi od 150 mg/L vode za piće i jopanoična kiselina (IA+) u dozi od 6 mg/100g t.m. intraperitonealno. Telesna masa oglednih pacova je merena svakih sedam dana. Nakon tri nedelje tretmana uzeti su uzorci krvi životinja iz svih oglednih grupa i određivani su sledeći parametri: koncentracja selena u punoj krvi, tiroksin (T4), trijodtironin (T3) i tireostimulirajući hormon (TSH) u krvnoj plazmi. Analiza je pokazala da su jedinke iz grupe tretiranih sa PTU imale nižu telesnu masu u odnosu na kontrolnu, kao i nižu koncentraciju T3 i T4 u plazmi. Selendeficitni pacovi su nakon tri nedelje imali nižu prosečnu telesnu masu u odnosu na selenadekvatne, ali nisu uočene razlike u koncentraciji tireoidnih hormona. Najniža prosečna telesna masa zabeležena je kod selendeficitnih grupa pacova tretiranih sa PTU.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - The influence of selenium and deiodinases blockers on juvenile rats body weight
T1  - Vlijanie selena i blokatorov dejodinazy na ves juvenil'nyh krys
T1  - Uticaj selena i blokatora dejodinaza na telesnu masu juvenilnih pacova
VL  - 68
IS  - 1-2
SP  - 3
EP  - 9
DO  - 10.2298/VETGL1402003M
ER  - 

@article{
author = "Milanović, Svetlana and Jovanović, Ivan B. and Bjelica, Jelena and Joksimović, Srđan and Stefanović, Srđan and Radović, Magdalena and Kirovski, Danijela and Valčić, Olivera and Gvozdić, Dragan",
year = "2014",
abstract = "In this work there was investigated the influence of selenium and deodinases blockers on juvenile rats body weight during three months. The experiment was carried out on 64 rats divided into eight groups with eight individual animals per group. Following groups were formed: 1. Se+PTU-IA- (control group), 2. Se+PTU+IA+, 3. Se+PTU+IA-, 4. Se+P-TU-IA+, 5. Se-PTU-IA-, 6. Se-PTU+IA+, 7. Se-PTU+IA- and 8. Se-PTU-IA+. The groups labeled (Se+) were selenium adequate and they were fed with food that contained 0.334 mg Se/kg. The groups labeled (Se-) were selenium deficient and obtained food with 0.031 mg Se/kg. As deiodinases blockers there were used propylthiouracil (PTU+) in a dose of 150 mg/L of drinking water and iopanoic acid (IA+) in a dose of 6 mg/100 g TM intraperitoneally. Body weight of experimental rats was measured every seven days. After three weeks of treatment there were taken blood samples of animals from all experimental groups and following parameters were determined: selenium concentration in blood, thyroxine (T4), triiodothyronine (T3) and thyroidstimulating hormone (TSH) in blood plasma. Analysis of the samples showed that the animals from the groups treated with PTU had lower body weight in regard to the control group, as well as lower concentration of T3 and T4 in plasma. Selenium deficient rats had lower average body weight compared to the selenium adequate ones after three weeks, but there were no differences in thyroid hormones concentration. The lowest average body weight was noticed in selenium deficient rats groups treated with PTU., U okviru ogleda je ispitivan uticaj selena i blokatora dejodinaza na telesnu masu juvenilnih pacova tokom tri nedelje. Ogled je izveden na 64 pacova podeljenih u osam grupa od po 8 jedinki. Formirane su sledeće grupe: 1. Se+PTU-IA- (kontrolna grupa), 2. Se+PTU+IA+, 3. Se+PTU+IA-, 4. Se+PTU-IA+, 5. Se-PTU-IA-, 6. Se-PTU+IA+, 7. Se-PTU+IA- i 8. Se-PTU-IA+. Grupe sa oznakom (Se+) su bile selenadekvatne i dobijale su hranu koja je sadržala 0,334 mg Se/kg hrane. Grupe sa oznakom (Se-) su bile selendeficitne i dobijale su 0,031 mg mg Se/kg hrane. Kao blokatori dejodinaza su korišćeni propiltiouracil (PTU+) u dozi od 150 mg/L vode za piće i jopanoična kiselina (IA+) u dozi od 6 mg/100g t.m. intraperitonealno. Telesna masa oglednih pacova je merena svakih sedam dana. Nakon tri nedelje tretmana uzeti su uzorci krvi životinja iz svih oglednih grupa i određivani su sledeći parametri: koncentracja selena u punoj krvi, tiroksin (T4), trijodtironin (T3) i tireostimulirajući hormon (TSH) u krvnoj plazmi. Analiza je pokazala da su jedinke iz grupe tretiranih sa PTU imale nižu telesnu masu u odnosu na kontrolnu, kao i nižu koncentraciju T3 i T4 u plazmi. Selendeficitni pacovi su nakon tri nedelje imali nižu prosečnu telesnu masu u odnosu na selenadekvatne, ali nisu uočene razlike u koncentraciji tireoidnih hormona. Najniža prosečna telesna masa zabeležena je kod selendeficitnih grupa pacova tretiranih sa PTU.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "The influence of selenium and deiodinases blockers on juvenile rats body weight, Vlijanie selena i blokatorov dejodinazy na ves juvenil'nyh krys, Uticaj selena i blokatora dejodinaza na telesnu masu juvenilnih pacova",
volume = "68",
number = "1-2",
pages = "3-9",
doi = "10.2298/VETGL1402003M"
}

Milanović, S., Jovanović, I. B., Bjelica, J., Joksimović, S., Stefanović, S., Radović, M., Kirovski, D., Valčić, O.,& Gvozdić, D.. (2014). The influence of selenium and deiodinases blockers on juvenile rats body weight. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(1-2), 3-9.
https://doi.org/10.2298/VETGL1402003M

Milanović S, Jovanović IB, Bjelica J, Joksimović S, Stefanović S, Radović M, Kirovski D, Valčić O, Gvozdić D. The influence of selenium and deiodinases blockers on juvenile rats body weight. in Veterinarski Glasnik. 2014;68(1-2):3-9.
doi:10.2298/VETGL1402003M .

Milanović, Svetlana, Jovanović, Ivan B., Bjelica, Jelena, Joksimović, Srđan, Stefanović, Srđan, Radović, Magdalena, Kirovski, Danijela, Valčić, Olivera, Gvozdić, Dragan, "The influence of selenium and deiodinases blockers on juvenile rats body weight" in Veterinarski Glasnik, 68, no. 1-2 (2014):3-9,
https://doi.org/10.2298/VETGL1402003M . .

TY  - JOUR
AU  - Janković, Saša
AU  - Antonijević, Biljana
AU  - Ćurčić, Marijana
AU  - Radičević, Tatjana
AU  - Stefanović, Srđan
AU  - Nikolić, Dragica M.
AU  - Ćupić, Vitomir
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/903
AB  - Nutritional benefits of fish can be attributed primarily to the content of high-quality proteins, vitamins, elements and omega-3 polyunsaturated fatty acids. On the other hand, fish and fishery products are susceptible to contamination by chemicals that have been recognized as ubiquitous environmental pollutants such as toxic elements and polychlorinated organic compounds. Fish consumption could be therefore considered as one of the major sources of human exposure to all above-mentioned environmental contaminants. This paper is focused on mercury (Hg) that enters the environment by both, natural means (such as volcanic activity, erosions and weathering, factors which contribute to the presence of Hg in water, soil and the atmosphere) and human activities - mining, fossil fuels combustion, industrial emissions, direct application of fertilizers and fungicides as well as disposal of solid waste. Total concentrations of Hg were measured in fish muscle and canned fish products available on Serbian market. Total of 651 samples were analyzed: 350 samples of marine fish (hake, mackerel, sprat, scorpanea, gilthead, salmon), 34 samples of freshwater fish (trout and carp) and 267 samples of canned fish products (tuna and sardines). Data were collected during 2011. For the purpose of intake assessment, we used the data obtained from the GEMS/Food Consumption Cluster Diets database. According to this source, estimated average weekly consumption of marine fish is 106.4 g/week, while freshwater fish and canned fish contribute to the consumption with 29.4 g and 18.2 g/week respectively. Mercury concentrations in marine fish were in the range of 0.005-0.208 μgg-1 (mean 0.040 μgg-1); in freshwater fish 0.005- 0.099 μgg-1 (mean 0.020 μgg-1) and in canned products they were in the range of 0.005-0.642 μgg-1 (mean 0.064 μgg-1). All analyzed samples contained mercury below the maximum level laid down by the European Union and Serbian regulation. The estimated weekly intake for total mercury, based on mean mercury value in fish and average body mass of 70 kg, was 0.095 μg/kg b.w./week. Based on FAO/WHO recommended safe limit and on obtained results, we can conclude that the intake of mercury in the case of consuming fish and canned fish products is lower than the safe limit.
AB  - Nutritivna korist od konzumacije ribe ogleda se, pre svega u sadržaju visoko vrednih proteina, vitamina, makro i mikroelemenata i omega-3 polinezasićenih masnih kiselina. Sa druge strane, riba i proizvodi od ribe su u značajnoj meri podložni hemijskoj kontaminaciji ubikvitarnim zagađivačima kao što su teški metal i polihlorovana organska jedinjenja. Zbog svega ovoga, konzumacija ribe se smatra jednim od najznačajnijih izvora izloženosti navedenim kontaminentima. U radu je ispitivana kontaminacija živom koja se u spoljašnjoj sredini može naći kao posledica prirodnih procesa (vulkanska aktivnost, erozija tla i klimatska dešavanja koja doprinose prisustvu žive u vodi, zemljištu i atmosferi), kao i aktivnosti čoveka - eksploatacija ruda, sagorevanje fosilnih goriva, emisija industrijskih gasova, direktna primena veštačkih đubriva i fungicida, kao i neadekvatno odlaganje čvrstog otpada. Sadržaj ukupne žive je određivan u svežoj i konzervisanoj ribi sa srpskog tržišta. Ispitan je 651 uzorak: 350 uzoraka morske ribe (oslić, skuša, sardela, škarpina, orada i losos), 34 uzorka slatkovodne ribe (šaran i pastrmka) i 267 uzoraka konzervisane ribe (tuna i sardela). Svi uzorci su analizirani tokom 2011. godine. Za procenu unosa korišćeni su podaci iz 'GEMS/Food Consumption Cluster Diets database'. Prema ovom izvoru, procenjena prosečna nedeljna konzumacija morske ribe iznosi 106,4 g, slatkovodne 29,4 g dok je ova vrednost za konzervisane proizvode od ribe 18,2 g. Sardžaj žive u morskoj ribi se kretao u opsegu od 0,005 do 0,208 μgg-1 (srednja vrednost 0,040 μgg-1); u slatkovodnoj ribi 0,005-0,099 μgg-1 (srednja vrednost 0,020 μgg-1), dok se u konzervisanim ribljim proizvodima sadržaj žive kretao od 0,005 do 0,642 μgg-1 (srednja vrednost 0,064 μgg-1). Nivo žive u svim ispitanim uzorcima je bio ispod maksimalno dozvoljenih vrednosti propisanih relevantnom legislativom EU kao i domaćim propisima. Procenjeni nedeljni unos žive baziran na srednjoj vrednosti žive u konzumiranoj ribi i prosečnoj telesnoj težini od 70 kg, bio je 0,095 μg/kg telesne mase nedeljno. Na osnovu preporuka FAO/WHO i dobijenih rezultata, može se zaključiti da je unos žive pri konzumaciji morske i slatkovodne ribe i konzervisanih ribljih proizvoda, niži od preporučenih graničnih vrednosti.
PB  - Institut za higijenu i tehnologiju mesa, Beograd
T2  - Tehnologija mesa
T1  - Assessment of mercury intake associated with fish consumption in Serbia
T1  - Procena unosa žive kroz konzumaciju ribe u Srbiji
VL  - 53
IS  - 1
SP  - 56
EP  - 61
DO  - 10.5937/tehmesa1201056J
ER  - 

@article{
author = "Janković, Saša and Antonijević, Biljana and Ćurčić, Marijana and Radičević, Tatjana and Stefanović, Srđan and Nikolić, Dragica M. and Ćupić, Vitomir",
year = "2012",
abstract = "Nutritional benefits of fish can be attributed primarily to the content of high-quality proteins, vitamins, elements and omega-3 polyunsaturated fatty acids. On the other hand, fish and fishery products are susceptible to contamination by chemicals that have been recognized as ubiquitous environmental pollutants such as toxic elements and polychlorinated organic compounds. Fish consumption could be therefore considered as one of the major sources of human exposure to all above-mentioned environmental contaminants. This paper is focused on mercury (Hg) that enters the environment by both, natural means (such as volcanic activity, erosions and weathering, factors which contribute to the presence of Hg in water, soil and the atmosphere) and human activities - mining, fossil fuels combustion, industrial emissions, direct application of fertilizers and fungicides as well as disposal of solid waste. Total concentrations of Hg were measured in fish muscle and canned fish products available on Serbian market. Total of 651 samples were analyzed: 350 samples of marine fish (hake, mackerel, sprat, scorpanea, gilthead, salmon), 34 samples of freshwater fish (trout and carp) and 267 samples of canned fish products (tuna and sardines). Data were collected during 2011. For the purpose of intake assessment, we used the data obtained from the GEMS/Food Consumption Cluster Diets database. According to this source, estimated average weekly consumption of marine fish is 106.4 g/week, while freshwater fish and canned fish contribute to the consumption with 29.4 g and 18.2 g/week respectively. Mercury concentrations in marine fish were in the range of 0.005-0.208 μgg-1 (mean 0.040 μgg-1); in freshwater fish 0.005- 0.099 μgg-1 (mean 0.020 μgg-1) and in canned products they were in the range of 0.005-0.642 μgg-1 (mean 0.064 μgg-1). All analyzed samples contained mercury below the maximum level laid down by the European Union and Serbian regulation. The estimated weekly intake for total mercury, based on mean mercury value in fish and average body mass of 70 kg, was 0.095 μg/kg b.w./week. Based on FAO/WHO recommended safe limit and on obtained results, we can conclude that the intake of mercury in the case of consuming fish and canned fish products is lower than the safe limit., Nutritivna korist od konzumacije ribe ogleda se, pre svega u sadržaju visoko vrednih proteina, vitamina, makro i mikroelemenata i omega-3 polinezasićenih masnih kiselina. Sa druge strane, riba i proizvodi od ribe su u značajnoj meri podložni hemijskoj kontaminaciji ubikvitarnim zagađivačima kao što su teški metal i polihlorovana organska jedinjenja. Zbog svega ovoga, konzumacija ribe se smatra jednim od najznačajnijih izvora izloženosti navedenim kontaminentima. U radu je ispitivana kontaminacija živom koja se u spoljašnjoj sredini može naći kao posledica prirodnih procesa (vulkanska aktivnost, erozija tla i klimatska dešavanja koja doprinose prisustvu žive u vodi, zemljištu i atmosferi), kao i aktivnosti čoveka - eksploatacija ruda, sagorevanje fosilnih goriva, emisija industrijskih gasova, direktna primena veštačkih đubriva i fungicida, kao i neadekvatno odlaganje čvrstog otpada. Sadržaj ukupne žive je određivan u svežoj i konzervisanoj ribi sa srpskog tržišta. Ispitan je 651 uzorak: 350 uzoraka morske ribe (oslić, skuša, sardela, škarpina, orada i losos), 34 uzorka slatkovodne ribe (šaran i pastrmka) i 267 uzoraka konzervisane ribe (tuna i sardela). Svi uzorci su analizirani tokom 2011. godine. Za procenu unosa korišćeni su podaci iz 'GEMS/Food Consumption Cluster Diets database'. Prema ovom izvoru, procenjena prosečna nedeljna konzumacija morske ribe iznosi 106,4 g, slatkovodne 29,4 g dok je ova vrednost za konzervisane proizvode od ribe 18,2 g. Sardžaj žive u morskoj ribi se kretao u opsegu od 0,005 do 0,208 μgg-1 (srednja vrednost 0,040 μgg-1); u slatkovodnoj ribi 0,005-0,099 μgg-1 (srednja vrednost 0,020 μgg-1), dok se u konzervisanim ribljim proizvodima sadržaj žive kretao od 0,005 do 0,642 μgg-1 (srednja vrednost 0,064 μgg-1). Nivo žive u svim ispitanim uzorcima je bio ispod maksimalno dozvoljenih vrednosti propisanih relevantnom legislativom EU kao i domaćim propisima. Procenjeni nedeljni unos žive baziran na srednjoj vrednosti žive u konzumiranoj ribi i prosečnoj telesnoj težini od 70 kg, bio je 0,095 μg/kg telesne mase nedeljno. Na osnovu preporuka FAO/WHO i dobijenih rezultata, može se zaključiti da je unos žive pri konzumaciji morske i slatkovodne ribe i konzervisanih ribljih proizvoda, niži od preporučenih graničnih vrednosti.",
publisher = "Institut za higijenu i tehnologiju mesa, Beograd",
journal = "Tehnologija mesa",
title = "Assessment of mercury intake associated with fish consumption in Serbia, Procena unosa žive kroz konzumaciju ribe u Srbiji",
volume = "53",
number = "1",
pages = "56-61",
doi = "10.5937/tehmesa1201056J"
}

Janković, S., Antonijević, B., Ćurčić, M., Radičević, T., Stefanović, S., Nikolić, D. M.,& Ćupić, V.. (2012). Assessment of mercury intake associated with fish consumption in Serbia. in Tehnologija mesa
Institut za higijenu i tehnologiju mesa, Beograd., 53(1), 56-61.
https://doi.org/10.5937/tehmesa1201056J

Janković S, Antonijević B, Ćurčić M, Radičević T, Stefanović S, Nikolić DM, Ćupić V. Assessment of mercury intake associated with fish consumption in Serbia. in Tehnologija mesa. 2012;53(1):56-61.
doi:10.5937/tehmesa1201056J .

Janković, Saša, Antonijević, Biljana, Ćurčić, Marijana, Radičević, Tatjana, Stefanović, Srđan, Nikolić, Dragica M., Ćupić, Vitomir, "Assessment of mercury intake associated with fish consumption in Serbia" in Tehnologija mesa, 53, no. 1 (2012):56-61,
https://doi.org/10.5937/tehmesa1201056J . .