TY  - JOUR
AU  - Velhner, Maja
AU  - Mitevski, Darko
AU  - Potkonjak, Dubravka
AU  - Stojanović, Dragica
AU  - Kovačević, Mira
AU  - Petrović, Tamaš
AU  - Aleksić-Kovačević, Sanja
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/706
AB  - The biological properties of an infectious bursal disease (IBD) virus isolated from bursas collected during an outbreak in a village chicken flock in Macedonia are described. The mortality rate was 50%. Two viruses coexisted in the bursas of infected chickens (IBDVwt and IBDVtc). The virus termed IBDVtc grows on chicken embryo fibroblast (CEF) cells from the first passage. Specific pathogen free chickens inoculated with IBDVtc at passage level 4 did not develop any clinical signs of disease. Some discrete bleeding on the leg muscles was seen and the bursa of Fabricius revealed pathological lesions similar to those caused by classical strains. However, the bursa recovered quickly (bursa lesion score 2) by 14 days post infection (PI). We also found evidence of bursal repopulation by means of perinuclear antigen staining. Strong CD3 influx was evident at 4 days PI, and at 33 days PI the CD3+ cell finding was comparable to the control. The mean antibody titre was 9.2 log(2) at 14 days PI. The amino acid composition of VP2 in IBDVwt (222 Ala, 242 Ile, 253 Gln, 256 Ile, 279 Asp, 284 Ala, 294 Ile and 299 Ser) is described. The same sequence was found in IBDVtc, except for two point mutations, at Gln253 -> His and Ala284 -> Thr. Such amino acid substitution is responsible for partial attenuation and the ability of the strain to replicate in cell culture. None of the commercial vaccine viruses has a similar arrangement of amino acids in the variable domain of IBDV. This strongly suggests that IBDVtc originates from a very virulent strain. To the best of our knowledge, this is the first report of a concomitant infection of chickens with highly pathogenic IBDV and its mutant counterpart.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Veterinaria Hungarica
T1  - Biological properties of a naturally attenuated infectious bursal disease virus isolated from a backyard chicken flock
VL  - 58
IS  - 4
SP  - 499
EP  - 509
DO  - 10.1556/AVet.58.2010.4.10
ER  - 

@article{
author = "Velhner, Maja and Mitevski, Darko and Potkonjak, Dubravka and Stojanović, Dragica and Kovačević, Mira and Petrović, Tamaš and Aleksić-Kovačević, Sanja",
year = "2010",
abstract = "The biological properties of an infectious bursal disease (IBD) virus isolated from bursas collected during an outbreak in a village chicken flock in Macedonia are described. The mortality rate was 50%. Two viruses coexisted in the bursas of infected chickens (IBDVwt and IBDVtc). The virus termed IBDVtc grows on chicken embryo fibroblast (CEF) cells from the first passage. Specific pathogen free chickens inoculated with IBDVtc at passage level 4 did not develop any clinical signs of disease. Some discrete bleeding on the leg muscles was seen and the bursa of Fabricius revealed pathological lesions similar to those caused by classical strains. However, the bursa recovered quickly (bursa lesion score 2) by 14 days post infection (PI). We also found evidence of bursal repopulation by means of perinuclear antigen staining. Strong CD3 influx was evident at 4 days PI, and at 33 days PI the CD3+ cell finding was comparable to the control. The mean antibody titre was 9.2 log(2) at 14 days PI. The amino acid composition of VP2 in IBDVwt (222 Ala, 242 Ile, 253 Gln, 256 Ile, 279 Asp, 284 Ala, 294 Ile and 299 Ser) is described. The same sequence was found in IBDVtc, except for two point mutations, at Gln253 -> His and Ala284 -> Thr. Such amino acid substitution is responsible for partial attenuation and the ability of the strain to replicate in cell culture. None of the commercial vaccine viruses has a similar arrangement of amino acids in the variable domain of IBDV. This strongly suggests that IBDVtc originates from a very virulent strain. To the best of our knowledge, this is the first report of a concomitant infection of chickens with highly pathogenic IBDV and its mutant counterpart.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "Biological properties of a naturally attenuated infectious bursal disease virus isolated from a backyard chicken flock",
volume = "58",
number = "4",
pages = "499-509",
doi = "10.1556/AVet.58.2010.4.10"
}

Velhner, M., Mitevski, D., Potkonjak, D., Stojanović, D., Kovačević, M., Petrović, T.,& Aleksić-Kovačević, S.. (2010). Biological properties of a naturally attenuated infectious bursal disease virus isolated from a backyard chicken flock. in Acta Veterinaria Hungarica
Akademiai Kiado Rt, Budapest., 58(4), 499-509.
https://doi.org/10.1556/AVet.58.2010.4.10

Velhner M, Mitevski D, Potkonjak D, Stojanović D, Kovačević M, Petrović T, Aleksić-Kovačević S. Biological properties of a naturally attenuated infectious bursal disease virus isolated from a backyard chicken flock. in Acta Veterinaria Hungarica. 2010;58(4):499-509.
doi:10.1556/AVet.58.2010.4.10 .

Velhner, Maja, Mitevski, Darko, Potkonjak, Dubravka, Stojanović, Dragica, Kovačević, Mira, Petrović, Tamaš, Aleksić-Kovačević, Sanja, "Biological properties of a naturally attenuated infectious bursal disease virus isolated from a backyard chicken flock" in Acta Veterinaria Hungarica, 58, no. 4 (2010):499-509,
https://doi.org/10.1556/AVet.58.2010.4.10 . .