TY  - JOUR
AU  - Stanković, B.
AU  - Surbatović, M.
AU  - Stojanović, G.
AU  - Mihajlović, M.
AU  - Tambur, Zoran
AU  - Kulišić, Zoran
AU  - Marković, M.
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/826
AB  - The basic aim of this work was to examine possibilities for introducing a new biotechnological treatment for preparation, storage and further purifying the suspension of leukocytes ("buffy coat" (BC)), intended for production of human interferon-alpha (hIFN-hIFN-α) and human interferon-gamma (hIFN-γ). Three hundred and eighty four units of BC were examined. They were divided into three series of 128 BC units each - one control series (KSBC) and two experimental series to which 10% dextran solution (ESBC-I) or 6% hydroxyethyl starch solution (ESBC-II) were added. The lowest titer (297929 IU) of hIFN-α ensued from KSBC, a higher titer from ESBC-I (317184 IU) and the highest titer from ESBC-II (364406 IU). We also found that the lowest hIFN- γ titer was produced from KSBC (48142 IU), a somewhat larger one from ESBC-I (67571 IU), and the highest titer was obtained from ESBC-II (73428. IU). This study has demonstrated the positive influence of modified biotechnological treatments on the production of hIFN-α and hIFN- γ from BC.
T2  - Scientific Research and Essays
T1  - Treatment for production of interferon-alpha (ifn-α) and interferon-gamma (ifn-γ) from the same purified suspension of leukocytes
VL  - 6
IS  - 7
SP  - 1522
EP  - 1529
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_826
ER  - 

@article{
author = "Stanković, B. and Surbatović, M. and Stojanović, G. and Mihajlović, M. and Tambur, Zoran and Kulišić, Zoran and Marković, M.",
year = "2011",
abstract = "The basic aim of this work was to examine possibilities for introducing a new biotechnological treatment for preparation, storage and further purifying the suspension of leukocytes ("buffy coat" (BC)), intended for production of human interferon-alpha (hIFN-hIFN-α) and human interferon-gamma (hIFN-γ). Three hundred and eighty four units of BC were examined. They were divided into three series of 128 BC units each - one control series (KSBC) and two experimental series to which 10% dextran solution (ESBC-I) or 6% hydroxyethyl starch solution (ESBC-II) were added. The lowest titer (297929 IU) of hIFN-α ensued from KSBC, a higher titer from ESBC-I (317184 IU) and the highest titer from ESBC-II (364406 IU). We also found that the lowest hIFN- γ titer was produced from KSBC (48142 IU), a somewhat larger one from ESBC-I (67571 IU), and the highest titer was obtained from ESBC-II (73428. IU). This study has demonstrated the positive influence of modified biotechnological treatments on the production of hIFN-α and hIFN- γ from BC.",
journal = "Scientific Research and Essays",
title = "Treatment for production of interferon-alpha (ifn-α) and interferon-gamma (ifn-γ) from the same purified suspension of leukocytes",
volume = "6",
number = "7",
pages = "1522-1529",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_826"
}

Stanković, B., Surbatović, M., Stojanović, G., Mihajlović, M., Tambur, Z., Kulišić, Z.,& Marković, M.. (2011). Treatment for production of interferon-alpha (ifn-α) and interferon-gamma (ifn-γ) from the same purified suspension of leukocytes. in Scientific Research and Essays, 6(7), 1522-1529.
https://hdl.handle.net/21.15107/rcub_veterinar_826

Stanković B, Surbatović M, Stojanović G, Mihajlović M, Tambur Z, Kulišić Z, Marković M. Treatment for production of interferon-alpha (ifn-α) and interferon-gamma (ifn-γ) from the same purified suspension of leukocytes. in Scientific Research and Essays. 2011;6(7):1522-1529.
https://hdl.handle.net/21.15107/rcub_veterinar_826 .

Stanković, B., Surbatović, M., Stojanović, G., Mihajlović, M., Tambur, Zoran, Kulišić, Zoran, Marković, M., "Treatment for production of interferon-alpha (ifn-α) and interferon-gamma (ifn-γ) from the same purified suspension of leukocytes" in Scientific Research and Essays, 6, no. 7 (2011):1522-1529,
https://hdl.handle.net/21.15107/rcub_veterinar_826 .