TY  - JOUR
AU  - Matović, Kazimir
AU  - Ćirić, Jelena
AU  - Kaljević, Vesna
AU  - Nedić, Nebojša
AU  - Jevtić, Goran
AU  - Vasković, Nikola
AU  - Baltić, Milan Ž.
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1660
AB  - Honey is a natural substance produced by honey bees (the genus Apis) enjoyed by people due to its unique nutritional and medicinal properties. The aim of this study was to determine the physicochemical parameters (moisture, ash, water-insoluble content, reducing sugars, sucrose, five acidity, diastase activity, hydroxymethylfurfural, and electrical conductivity) and microbiological status (total number of aerobic mesophilic bacteria, total number of sulfite-reducing clostridia, the presence of Salmonella spp., total numbers of fungi and yeasts and the presence of Clostridium botulinum) in honey (honeydew, blossom, sunflower, acacia, and linden) produced in an urban environment in Serbia. We analyzed 19 apiary samples of honey, collected during the 2011 harvesting season, by using recommendation methods. Physicochemical parameters of the examined honey produced in the urban environment indicated the honeys were of acceptable quality. Bacillus spp. were detected in four honeys, yeasts were detected in three honeys, and Clostridium botulinum type E was detected in one honey using PCR. The current study also showed the presence of diverse honey varieties in Serbia.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia
VL  - 25
IS  - 14
SP  - 14148
EP  - 14157
DO  - 10.1007/s11356-018-1659-1
ER  - 

@article{
author = "Matović, Kazimir and Ćirić, Jelena and Kaljević, Vesna and Nedić, Nebojša and Jevtić, Goran and Vasković, Nikola and Baltić, Milan Ž.",
year = "2018",
abstract = "Honey is a natural substance produced by honey bees (the genus Apis) enjoyed by people due to its unique nutritional and medicinal properties. The aim of this study was to determine the physicochemical parameters (moisture, ash, water-insoluble content, reducing sugars, sucrose, five acidity, diastase activity, hydroxymethylfurfural, and electrical conductivity) and microbiological status (total number of aerobic mesophilic bacteria, total number of sulfite-reducing clostridia, the presence of Salmonella spp., total numbers of fungi and yeasts and the presence of Clostridium botulinum) in honey (honeydew, blossom, sunflower, acacia, and linden) produced in an urban environment in Serbia. We analyzed 19 apiary samples of honey, collected during the 2011 harvesting season, by using recommendation methods. Physicochemical parameters of the examined honey produced in the urban environment indicated the honeys were of acceptable quality. Bacillus spp. were detected in four honeys, yeasts were detected in three honeys, and Clostridium botulinum type E was detected in one honey using PCR. The current study also showed the presence of diverse honey varieties in Serbia.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia",
volume = "25",
number = "14",
pages = "14148-14157",
doi = "10.1007/s11356-018-1659-1"
}

Matović, K., Ćirić, J., Kaljević, V., Nedić, N., Jevtić, G., Vasković, N.,& Baltić, M. Ž.. (2018). Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 25(14), 14148-14157.
https://doi.org/10.1007/s11356-018-1659-1

Matović K, Ćirić J, Kaljević V, Nedić N, Jevtić G, Vasković N, Baltić MŽ. Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia. in Environmental Science and Pollution Research. 2018;25(14):14148-14157.
doi:10.1007/s11356-018-1659-1 .

Matović, Kazimir, Ćirić, Jelena, Kaljević, Vesna, Nedić, Nebojša, Jevtić, Goran, Vasković, Nikola, Baltić, Milan Ž., "Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia" in Environmental Science and Pollution Research, 25, no. 14 (2018):14148-14157,
https://doi.org/10.1007/s11356-018-1659-1 . .

TY  - CONF
AU  - Matović, Kazimir
AU  - Baltić, Milan Ž.
AU  - Nedić, Nebojša
AU  - Dmitrić, Marko
AU  - Nenadić, Dragan
AU  - Vasković, Nikola
AU  - Jevtić, Goran
AU  - Mišić, Dušan
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1267
AB  - The presence of Clostridium botulinum spores in 59 honey samples originating from different regions of the Republic of Serbia was studied. In addition to microbiological methods, after enrichment, centrifugation and membrane filtration, molecular methods (PCR methods) were utilized. The number of spores in PCR positive samples was estimated by the most probable number (MPN) method. PCR confirmed C. botulinum spores in 5 (8.47%) honey samples. MPN of spores varied from 20/kg to 204/kg honey. PCR was more sensitive than cultural methods. Natural honey contamination with C. botulinum spores is low-level and not homogeneous, and therefore, PCR methods require multiple sub-sampling.
PB  - Elsevier Science Bv, Amsterdam
C3  - 58th International Meat Industry Conference (MEATCON2015)
T1  - The investigation of the presence of Clostridium botulinum spores in honey in Serbia
VL  - 5
SP  - 180
EP  - 183
DO  - 10.1016/j.profoo.2015.09.051
ER  - 

@conference{
author = "Matović, Kazimir and Baltić, Milan Ž. and Nedić, Nebojša and Dmitrić, Marko and Nenadić, Dragan and Vasković, Nikola and Jevtić, Goran and Mišić, Dušan",
year = "2015",
abstract = "The presence of Clostridium botulinum spores in 59 honey samples originating from different regions of the Republic of Serbia was studied. In addition to microbiological methods, after enrichment, centrifugation and membrane filtration, molecular methods (PCR methods) were utilized. The number of spores in PCR positive samples was estimated by the most probable number (MPN) method. PCR confirmed C. botulinum spores in 5 (8.47%) honey samples. MPN of spores varied from 20/kg to 204/kg honey. PCR was more sensitive than cultural methods. Natural honey contamination with C. botulinum spores is low-level and not homogeneous, and therefore, PCR methods require multiple sub-sampling.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "58th International Meat Industry Conference (MEATCON2015)",
title = "The investigation of the presence of Clostridium botulinum spores in honey in Serbia",
volume = "5",
pages = "180-183",
doi = "10.1016/j.profoo.2015.09.051"
}

Matović, K., Baltić, M. Ž., Nedić, N., Dmitrić, M., Nenadić, D., Vasković, N., Jevtić, G.,& Mišić, D.. (2015). The investigation of the presence of Clostridium botulinum spores in honey in Serbia. in 58th International Meat Industry Conference (MEATCON2015)
Elsevier Science Bv, Amsterdam., 5, 180-183.
https://doi.org/10.1016/j.profoo.2015.09.051

Matović K, Baltić MŽ, Nedić N, Dmitrić M, Nenadić D, Vasković N, Jevtić G, Mišić D. The investigation of the presence of Clostridium botulinum spores in honey in Serbia. in 58th International Meat Industry Conference (MEATCON2015). 2015;5:180-183.
doi:10.1016/j.profoo.2015.09.051 .

Matović, Kazimir, Baltić, Milan Ž., Nedić, Nebojša, Dmitrić, Marko, Nenadić, Dragan, Vasković, Nikola, Jevtić, Goran, Mišić, Dušan, "The investigation of the presence of Clostridium botulinum spores in honey in Serbia" in 58th International Meat Industry Conference (MEATCON2015), 5 (2015):180-183,
https://doi.org/10.1016/j.profoo.2015.09.051 . .

TY  - CONF
AU  - Matović, Kazimir
AU  - Baltić, Milan Ž.
AU  - Mišić, Dušan
AU  - Nedić, Nebojša
AU  - Karabasil, Nedjeljko
AU  - Ranin, Lazar
AU  - Ivanović, Jelena
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3750
AB  - U cilju kvalitativno kvantitativnog utvrđivanja prisustva mikroorganizama, u uzorcima meda, koriste se klasiče i međunarodno priznate, standardne (ISO) metode izolacije, identifikacije, kvantifikacije i determinacije.
Početkom dvadesetprvog veka a u cilju dobijanja brzih i pouzdanih rezultata laboratoriskih ispitivanja pristupa se izvođenju molekularnih metoda u uvrđivanju prisustva mikroorganizama u uzorcima meda. Navedene metode koristile su se za potvrdu i determinaciju mikroorganizama već izolovanih klasičnim i standardnim mikrobiološkim metodama ali i za direktno utvrđivanje prisustva mikroorganizama u ispitivanim uzorcima. U navedena ispitivanja uključena su ponovljena ispitivanja zbog fizičko-hemijskih karakteristika meda odnosno neravnomerne raspoređenosti mikroorganizama u ispitivanim uzorcima i mogućnosti dobijanja lažno negativnih rezultata. Molekularni pristupi u dijagnostici uključivali su osetljive i specifične testove lančane reakcije polimeraze (PCR), lančane reakcije polimeraze u realnom vremenu (Real-Time PCR) i metode gel-elektroforeze u pulsirajućem električnom polju (Pulse-Field Gel Elektrophoresis-PFGE). Detekcija mikroorganizama direktno iz neobrađenih uzoraka meda nije moguća. Zbog prisustva malog broja mikroorganizama, uglavnom spora, neophodna je priprema uzoraka meda koja mora uključiti postupak dilucije, predobogaćenja, centrifugovanja i membranske filtracije. Ovako dobijeni sadržaj koristi se za izolaciju ciljanih mikroorganizama klasičnim i standardnim metodama laboratorijske dijagnostike, odnosno za prečišćavanje i ekstrakciju nukleinskih kiselina za molekularne metode dijagnostike. U cilju detekcije mikroorganizama, u uzorcima meda, primenom molekularnih metoda dijagnostike, za svaki ispitujući uzorak moraju se izvoditi višestruka ponovljena ispitivanja zbog neravnomerne raspoređenosti mikroorganizama/spora i mogućnosti dobijanja lažno negativnih rezultata.
U uzorcima meda kontaminiranim referentnim sojevima mikroorganizama, molekularnim metodama može da se dokaže prisustvo jedne ćelije/spore mikroorganizma u jednom gramu meda dok je to nemoguće dokazati klasičnim/standardnim metodama. Klasične i standardne metode mikrobiološke dijagnostike nisu podesne za detekciju mikroorganizama u uzorcima meda jer ove metode zbog niske osetljivosti daju lažno negativne rezultate.
PB  - Beograd : Fakultet veterinarske medicine
C3  - 4. simpozijum - Bezbednost i kvalitet namirnica animalnog porekla, Beograd, 6-7. novembar 2014
T1  - Primena molekularnih tehnika dijagnostike u mikrobiološkoj analizi uzoraka meda
SP  - 90
EP  - 102
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3750
ER  - 

@conference{
author = "Matović, Kazimir and Baltić, Milan Ž. and Mišić, Dušan and Nedić, Nebojša and Karabasil, Nedjeljko and Ranin, Lazar and Ivanović, Jelena",
year = "2014",
abstract = "U cilju kvalitativno kvantitativnog utvrđivanja prisustva mikroorganizama, u uzorcima meda, koriste se klasiče i međunarodno priznate, standardne (ISO) metode izolacije, identifikacije, kvantifikacije i determinacije.
Početkom dvadesetprvog veka a u cilju dobijanja brzih i pouzdanih rezultata laboratoriskih ispitivanja pristupa se izvođenju molekularnih metoda u uvrđivanju prisustva mikroorganizama u uzorcima meda. Navedene metode koristile su se za potvrdu i determinaciju mikroorganizama već izolovanih klasičnim i standardnim mikrobiološkim metodama ali i za direktno utvrđivanje prisustva mikroorganizama u ispitivanim uzorcima. U navedena ispitivanja uključena su ponovljena ispitivanja zbog fizičko-hemijskih karakteristika meda odnosno neravnomerne raspoređenosti mikroorganizama u ispitivanim uzorcima i mogućnosti dobijanja lažno negativnih rezultata. Molekularni pristupi u dijagnostici uključivali su osetljive i specifične testove lančane reakcije polimeraze (PCR), lančane reakcije polimeraze u realnom vremenu (Real-Time PCR) i metode gel-elektroforeze u pulsirajućem električnom polju (Pulse-Field Gel Elektrophoresis-PFGE). Detekcija mikroorganizama direktno iz neobrađenih uzoraka meda nije moguća. Zbog prisustva malog broja mikroorganizama, uglavnom spora, neophodna je priprema uzoraka meda koja mora uključiti postupak dilucije, predobogaćenja, centrifugovanja i membranske filtracije. Ovako dobijeni sadržaj koristi se za izolaciju ciljanih mikroorganizama klasičnim i standardnim metodama laboratorijske dijagnostike, odnosno za prečišćavanje i ekstrakciju nukleinskih kiselina za molekularne metode dijagnostike. U cilju detekcije mikroorganizama, u uzorcima meda, primenom molekularnih metoda dijagnostike, za svaki ispitujući uzorak moraju se izvoditi višestruka ponovljena ispitivanja zbog neravnomerne raspoređenosti mikroorganizama/spora i mogućnosti dobijanja lažno negativnih rezultata.
U uzorcima meda kontaminiranim referentnim sojevima mikroorganizama, molekularnim metodama može da se dokaže prisustvo jedne ćelije/spore mikroorganizma u jednom gramu meda dok je to nemoguće dokazati klasičnim/standardnim metodama. Klasične i standardne metode mikrobiološke dijagnostike nisu podesne za detekciju mikroorganizama u uzorcima meda jer ove metode zbog niske osetljivosti daju lažno negativne rezultate.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "4. simpozijum - Bezbednost i kvalitet namirnica animalnog porekla, Beograd, 6-7. novembar 2014",
title = "Primena molekularnih tehnika dijagnostike u mikrobiološkoj analizi uzoraka meda",
pages = "90-102",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3750"
}

Matović, K., Baltić, M. Ž., Mišić, D., Nedić, N., Karabasil, N., Ranin, L.,& Ivanović, J.. (2014). Primena molekularnih tehnika dijagnostike u mikrobiološkoj analizi uzoraka meda. in 4. simpozijum - Bezbednost i kvalitet namirnica animalnog porekla, Beograd, 6-7. novembar 2014
Beograd : Fakultet veterinarske medicine., 90-102.
https://hdl.handle.net/21.15107/rcub_veterinar_3750

Matović K, Baltić MŽ, Mišić D, Nedić N, Karabasil N, Ranin L, Ivanović J. Primena molekularnih tehnika dijagnostike u mikrobiološkoj analizi uzoraka meda. in 4. simpozijum - Bezbednost i kvalitet namirnica animalnog porekla, Beograd, 6-7. novembar 2014. 2014;:90-102.
https://hdl.handle.net/21.15107/rcub_veterinar_3750 .

Matović, Kazimir, Baltić, Milan Ž., Mišić, Dušan, Nedić, Nebojša, Karabasil, Nedjeljko, Ranin, Lazar, Ivanović, Jelena, "Primena molekularnih tehnika dijagnostike u mikrobiološkoj analizi uzoraka meda" in 4. simpozijum - Bezbednost i kvalitet namirnica animalnog porekla, Beograd, 6-7. novembar 2014 (2014):90-102,
https://hdl.handle.net/21.15107/rcub_veterinar_3750 .