TY  - JOUR
AU  - Milošević, Ivan
AU  - Radovanović, Anita
AU  - Danilović-Luković, Jelena
AU  - Lužajić Božinovski, Tijana
AU  - Sourice-Petit, Sophie
AU  - Beck-Cormier, Sarah
AU  - Guicheux, Jerome
AU  - Vejnović, Branislav
AU  - Kovačević-Filipović, Milica
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1588
AB  - Maternal hypothyroidism in its overt form affects skeletal development of the offspring, but these data are not available for the subclinical form which is becoming very frequent among pregnant women. We hypothesized that the subclinical form of hypothyroidism in rat dams, influences the process of offspring endochondral ossification affecting proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups. Seven-day-old male pups (n=18) derived from control dams and dams treated with a low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water during pregnancy and lactation were used. Histomorphometric analysis of pups tibia proximal growth plate, expression of mRNA, immunohistochemical and histochemical visualization of extracellular matrix components was performed. The length of the tibia was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical and overt form were lower while the amount of glycosaminoglycans was higher when compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA indicated altered osteoclasts function while lower expression of dentin matrix acid protein-1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised bone formation in the overt form of hypothyroidism. The subclinical form of maternal hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in growth plate formation.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation
VL  - 68
IS  - 3
SP  - 301
EP  - 320
DO  - 10.2478/acve-2018-0026
ER  - 

@article{
author = "Milošević, Ivan and Radovanović, Anita and Danilović-Luković, Jelena and Lužajić Božinovski, Tijana and Sourice-Petit, Sophie and Beck-Cormier, Sarah and Guicheux, Jerome and Vejnović, Branislav and Kovačević-Filipović, Milica",
year = "2018",
abstract = "Maternal hypothyroidism in its overt form affects skeletal development of the offspring, but these data are not available for the subclinical form which is becoming very frequent among pregnant women. We hypothesized that the subclinical form of hypothyroidism in rat dams, influences the process of offspring endochondral ossification affecting proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups. Seven-day-old male pups (n=18) derived from control dams and dams treated with a low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water during pregnancy and lactation were used. Histomorphometric analysis of pups tibia proximal growth plate, expression of mRNA, immunohistochemical and histochemical visualization of extracellular matrix components was performed. The length of the tibia was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical and overt form were lower while the amount of glycosaminoglycans was higher when compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA indicated altered osteoclasts function while lower expression of dentin matrix acid protein-1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised bone formation in the overt form of hypothyroidism. The subclinical form of maternal hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in growth plate formation.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation",
volume = "68",
number = "3",
pages = "301-320",
doi = "10.2478/acve-2018-0026"
}

Milošević, I., Radovanović, A., Danilović-Luković, J., Lužajić Božinovski, T., Sourice-Petit, S., Beck-Cormier, S., Guicheux, J., Vejnović, B.,& Kovačević-Filipović, M.. (2018). Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(3), 301-320.
https://doi.org/10.2478/acve-2018-0026

Milošević I, Radovanović A, Danilović-Luković J, Lužajić Božinovski T, Sourice-Petit S, Beck-Cormier S, Guicheux J, Vejnović B, Kovačević-Filipović M. Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation. in Acta Veterinaria-Beograd. 2018;68(3):301-320.
doi:10.2478/acve-2018-0026 .

Milošević, Ivan, Radovanović, Anita, Danilović-Luković, Jelena, Lužajić Božinovski, Tijana, Sourice-Petit, Sophie, Beck-Cormier, Sarah, Guicheux, Jerome, Vejnović, Branislav, Kovačević-Filipović, Milica, "Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation" in Acta Veterinaria-Beograd, 68, no. 3 (2018):301-320,
https://doi.org/10.2478/acve-2018-0026 . .

TY  - JOUR
AU  - Francuski, Jelena
AU  - Debeljak-Martačić, Jasmina
AU  - Radovanović, Anita
AU  - Andrić, Nenad
AU  - Sourice-Petit, Sophie
AU  - Guicheux, Jerome
AU  - Mojsilović, Slavko
AU  - Kovačević-Filipović, Milica
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1171
AB  - The aim of this study was to determine whether synovial fluid (SF) of dogs contains cells that have characteristics of MSCs and to describe their differentiation potential. SF adherent cells from 5 young German shepherd dogs (average 3.8 +/- 0.9 years) were expanded (37 degrees C, 5% CO2, humidified atmosphere) three weeks before their phenotype was characterized by flow-cytometry for the presence of CD90 and CD34. Population doubling time (PDT), number of CFU-F and adipogenic, osteogenic and chondrogenic potentials have been determined in vitro. In early passages PTD was 31 +/- 10 hours and expansion fold after 3 sub cultivations (9 days) theoretically could be 372 +/- 134. At P1, 0.55 +/- 0.05% of SF cells had the ability to form CFU-F. Sixty-six percent of cells expressed CD90 and none of the cells expressed markers of hematopoietic cells. Oil Red O staining has shown accumulation of fat droplets in cells grown in adipogenic medium, while deposits of calcium in the osteogenic medium were evidenced with Alizarin red staining. SF cultured in hondrogenic and control medium in three-dimensional conditions formed a cartilage-like tissue. Alcian blue staining of pellets slides have shown a significant amount of glycosaminoglycans (GAGs) and immunohistochemistry analysis documented collagen type II expression. The amount of GAGs in pellets grown in both conditions showed no difference. SF cells in vitro exhibited osteogenic, adipogenic and chondrogenic differentiation potentials, suggesting the presence of different mesenchymal progenitors. These results also demonstrated that SF cells have a spontaneous chondrogenic potential that should be further explored for possible tissue engineering protocols.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Proliferation and differentiation potential of canine synovial fluid cells
VL  - 65
IS  - 1
SP  - 66
EP  - 78
DO  - 10.1515/acve-2015-0005
ER  - 

@article{
author = "Francuski, Jelena and Debeljak-Martačić, Jasmina and Radovanović, Anita and Andrić, Nenad and Sourice-Petit, Sophie and Guicheux, Jerome and Mojsilović, Slavko and Kovačević-Filipović, Milica",
year = "2015",
abstract = "The aim of this study was to determine whether synovial fluid (SF) of dogs contains cells that have characteristics of MSCs and to describe their differentiation potential. SF adherent cells from 5 young German shepherd dogs (average 3.8 +/- 0.9 years) were expanded (37 degrees C, 5% CO2, humidified atmosphere) three weeks before their phenotype was characterized by flow-cytometry for the presence of CD90 and CD34. Population doubling time (PDT), number of CFU-F and adipogenic, osteogenic and chondrogenic potentials have been determined in vitro. In early passages PTD was 31 +/- 10 hours and expansion fold after 3 sub cultivations (9 days) theoretically could be 372 +/- 134. At P1, 0.55 +/- 0.05% of SF cells had the ability to form CFU-F. Sixty-six percent of cells expressed CD90 and none of the cells expressed markers of hematopoietic cells. Oil Red O staining has shown accumulation of fat droplets in cells grown in adipogenic medium, while deposits of calcium in the osteogenic medium were evidenced with Alizarin red staining. SF cultured in hondrogenic and control medium in three-dimensional conditions formed a cartilage-like tissue. Alcian blue staining of pellets slides have shown a significant amount of glycosaminoglycans (GAGs) and immunohistochemistry analysis documented collagen type II expression. The amount of GAGs in pellets grown in both conditions showed no difference. SF cells in vitro exhibited osteogenic, adipogenic and chondrogenic differentiation potentials, suggesting the presence of different mesenchymal progenitors. These results also demonstrated that SF cells have a spontaneous chondrogenic potential that should be further explored for possible tissue engineering protocols.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Proliferation and differentiation potential of canine synovial fluid cells",
volume = "65",
number = "1",
pages = "66-78",
doi = "10.1515/acve-2015-0005"
}

Francuski, J., Debeljak-Martačić, J., Radovanović, A., Andrić, N., Sourice-Petit, S., Guicheux, J., Mojsilović, S.,& Kovačević-Filipović, M.. (2015). Proliferation and differentiation potential of canine synovial fluid cells. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(1), 66-78.
https://doi.org/10.1515/acve-2015-0005

Francuski J, Debeljak-Martačić J, Radovanović A, Andrić N, Sourice-Petit S, Guicheux J, Mojsilović S, Kovačević-Filipović M. Proliferation and differentiation potential of canine synovial fluid cells. in Acta Veterinaria-Beograd. 2015;65(1):66-78.
doi:10.1515/acve-2015-0005 .

Francuski, Jelena, Debeljak-Martačić, Jasmina, Radovanović, Anita, Andrić, Nenad, Sourice-Petit, Sophie, Guicheux, Jerome, Mojsilović, Slavko, Kovačević-Filipović, Milica, "Proliferation and differentiation potential of canine synovial fluid cells" in Acta Veterinaria-Beograd, 65, no. 1 (2015):66-78,
https://doi.org/10.1515/acve-2015-0005 . .

TY  - JOUR
AU  - Francuski, Jelena
AU  - Radovanović, Anita
AU  - Andrić, Nenad
AU  - Krstić, Vanja
AU  - Bogdanović, Dragan
AU  - Hadzić, V.
AU  - Todorović, V.
AU  - Lazarević Macanović, Mirjana
AU  - Petit, S. Sourice
AU  - Beck-Cormier, Sarah
AU  - Guicheux, J.
AU  - Gauthier, O.
AU  - Kovačević-Filipović, Milica
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1093
AB  - The aims of this study were to define age-related histological changes in the articular cartilage of the stifle joint in non-chondrodystrophic dogs and to determine whether physical activity has a positive impact on preservation of cartilage structure during ageing. Twenty-eight German shepherd dogs were included in the study. These dogs had no evidence of joint inflammation as defined by clinical assessment, radiology and synovial fluid analysis (specifically absence of synovial fluid serum amyloid A). The dogs were grouped as young working (n = 4), young non-working (n = 5), aged working (n = 13) and aged non-working (n = 6) animals. Gross changes in the stifle joints were recorded and biopsy samples of femoral and tibial articular cartilage were evaluated for thickness; chondrocyte number, density, surface area and morphology; isogenous group morphology; tidemark integrity; subchondral bone structure; presence of proteoglycans/glycosaminoglycans; and expression of type I, II and X collagens. The major age-related changes, not related to type of physical activity, included elevated chondrocyte density and thinning of tibial cartilage and increased chondrocyte surface area in the superficial and intermediate zone of the femoral cartilage. There was also expression of type X collagen in the femoral and tibial calcified and non-calcified cartilage; however, type X collagen was not detected in the superficial zone of old working dogs. Therefore, ageing, with or without physical activity, leads to slight cartilage degeneration, while physical activity modulates the synthesis of type X collagen in the superficial cartilage zone, partially preserving the structure of hyaline cartilage.
PB  - Elsevier Sci Ltd, Oxford
T2  - Journal of Comparative Pathology
T1  - Age-related Changes in the Articular Cartilage of the Stifle Joint in Non-working and Working German Shepherd Dogs
VL  - 151
IS  - 4
SP  - 363
EP  - 374
DO  - 10.1016/j.jcpa.2014.09.002
ER  - 

@article{
author = "Francuski, Jelena and Radovanović, Anita and Andrić, Nenad and Krstić, Vanja and Bogdanović, Dragan and Hadzić, V. and Todorović, V. and Lazarević Macanović, Mirjana and Petit, S. Sourice and Beck-Cormier, Sarah and Guicheux, J. and Gauthier, O. and Kovačević-Filipović, Milica",
year = "2014",
abstract = "The aims of this study were to define age-related histological changes in the articular cartilage of the stifle joint in non-chondrodystrophic dogs and to determine whether physical activity has a positive impact on preservation of cartilage structure during ageing. Twenty-eight German shepherd dogs were included in the study. These dogs had no evidence of joint inflammation as defined by clinical assessment, radiology and synovial fluid analysis (specifically absence of synovial fluid serum amyloid A). The dogs were grouped as young working (n = 4), young non-working (n = 5), aged working (n = 13) and aged non-working (n = 6) animals. Gross changes in the stifle joints were recorded and biopsy samples of femoral and tibial articular cartilage were evaluated for thickness; chondrocyte number, density, surface area and morphology; isogenous group morphology; tidemark integrity; subchondral bone structure; presence of proteoglycans/glycosaminoglycans; and expression of type I, II and X collagens. The major age-related changes, not related to type of physical activity, included elevated chondrocyte density and thinning of tibial cartilage and increased chondrocyte surface area in the superficial and intermediate zone of the femoral cartilage. There was also expression of type X collagen in the femoral and tibial calcified and non-calcified cartilage; however, type X collagen was not detected in the superficial zone of old working dogs. Therefore, ageing, with or without physical activity, leads to slight cartilage degeneration, while physical activity modulates the synthesis of type X collagen in the superficial cartilage zone, partially preserving the structure of hyaline cartilage.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Journal of Comparative Pathology",
title = "Age-related Changes in the Articular Cartilage of the Stifle Joint in Non-working and Working German Shepherd Dogs",
volume = "151",
number = "4",
pages = "363-374",
doi = "10.1016/j.jcpa.2014.09.002"
}

Francuski, J., Radovanović, A., Andrić, N., Krstić, V., Bogdanović, D., Hadzić, V., Todorović, V., Lazarević Macanović, M., Petit, S. S., Beck-Cormier, S., Guicheux, J., Gauthier, O.,& Kovačević-Filipović, M.. (2014). Age-related Changes in the Articular Cartilage of the Stifle Joint in Non-working and Working German Shepherd Dogs. in Journal of Comparative Pathology
Elsevier Sci Ltd, Oxford., 151(4), 363-374.
https://doi.org/10.1016/j.jcpa.2014.09.002

Francuski J, Radovanović A, Andrić N, Krstić V, Bogdanović D, Hadzić V, Todorović V, Lazarević Macanović M, Petit SS, Beck-Cormier S, Guicheux J, Gauthier O, Kovačević-Filipović M. Age-related Changes in the Articular Cartilage of the Stifle Joint in Non-working and Working German Shepherd Dogs. in Journal of Comparative Pathology. 2014;151(4):363-374.
doi:10.1016/j.jcpa.2014.09.002 .

Francuski, Jelena, Radovanović, Anita, Andrić, Nenad, Krstić, Vanja, Bogdanović, Dragan, Hadzić, V., Todorović, V., Lazarević Macanović, Mirjana, Petit, S. Sourice, Beck-Cormier, Sarah, Guicheux, J., Gauthier, O., Kovačević-Filipović, Milica, "Age-related Changes in the Articular Cartilage of the Stifle Joint in Non-working and Working German Shepherd Dogs" in Journal of Comparative Pathology, 151, no. 4 (2014):363-374,
https://doi.org/10.1016/j.jcpa.2014.09.002 . .