TY  - JOUR
AU  - Pantelić, M.
AU  - Jovanović, Ljubomir
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Durić, M.
AU  - Culafić, T.
AU  - Vranješ-Đurić, Sanja
AU  - Korićanac, Goran
AU  - Kirovski, Danijela
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1629
AB  - Thirty days before expected time of parturition, 20 Holstein cows were divided into -Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days -30, -10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days -10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days -28, -7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of beta-subunit of insulin receptor (IR beta) was significantly higher (p < 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser(307)) was significantly lower (p < 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser(307) was significantly lower in muscle tissue at days 7 and 28 (p < 0.05 and p < 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser(473)) was significantly higher (p < 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods.
PB  - Wiley, Hoboken
T2  - Journal of Animal Physiology and Animal Nutrition
T1  - The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows
VL  - 102
IS  - 1
SP  - 41
EP  - 55
DO  - 10.1111/jpn.12655
ER  - 

@article{
author = "Pantelić, M. and Jovanović, Ljubomir and Prodanović, Radiša and Vujanac, Ivan and Durić, M. and Culafić, T. and Vranješ-Đurić, Sanja and Korićanac, Goran and Kirovski, Danijela",
year = "2018",
abstract = "Thirty days before expected time of parturition, 20 Holstein cows were divided into -Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days -30, -10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days -10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days -28, -7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of beta-subunit of insulin receptor (IR beta) was significantly higher (p < 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser(307)) was significantly lower (p < 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser(307) was significantly lower in muscle tissue at days 7 and 28 (p < 0.05 and p < 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser(473)) was significantly higher (p < 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods.",
publisher = "Wiley, Hoboken",
journal = "Journal of Animal Physiology and Animal Nutrition",
title = "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows",
volume = "102",
number = "1",
pages = "41-55",
doi = "10.1111/jpn.12655"
}

Pantelić, M., Jovanović, L., Prodanović, R., Vujanac, I., Durić, M., Culafić, T., Vranješ-Đurić, S., Korićanac, G.,& Kirovski, D.. (2018). The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition
Wiley, Hoboken., 102(1), 41-55.
https://doi.org/10.1111/jpn.12655

Pantelić M, Jovanović L, Prodanović R, Vujanac I, Durić M, Culafić T, Vranješ-Đurić S, Korićanac G, Kirovski D. The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition. 2018;102(1):41-55.
doi:10.1111/jpn.12655 .

Pantelić, M., Jovanović, Ljubomir, Prodanović, Radiša, Vujanac, Ivan, Durić, M., Culafić, T., Vranješ-Đurić, Sanja, Korićanac, Goran, Kirovski, Danijela, "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows" in Journal of Animal Physiology and Animal Nutrition, 102, no. 1 (2018):41-55,
https://doi.org/10.1111/jpn.12655 . .

TY  - JOUR
AU  - Jovanović, Ljubomir
AU  - Pantelić, Marija
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Đurić, Miloje
AU  - Tepavcević, Snežana
AU  - Vranješ-Đurić, Sanja
AU  - Korićanac, Goran
AU  - Kirovski, Danijela
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1539
AB  - The objective of this study was to investigate the effects of peroral administration of chromium-enriched yeast on glucose tolerance in Holstein calves, assessed by insulin signaling pathway molecule determination and intravenous glucose tolerance test (IVGTT). Twenty-four Holstein calves, aged 1 month, were chosen for the study and divided into two groups: the PoCr group (n = 12) that perorally received 0.04 mg of Cr/kg of body mass daily, for 70 days, and the NCr group (n = 12) that received no chromium supplementation. Skeletal tissue samples from each calf were obtained on day 0 and day 70 of the experiment. Chromium supplementation increased protein content of the insulin beta-subunit receptor, phosphorylation of insulin receptor substrate 1 at Tyrosine 632, phosphorylation of Akt at Serine 473, glucose transporter-4, and AMP-activated protein kinase in skeletal muscle tissue, while phosphorylation of insulin receptor substrate 1 at Serine 307 was not affected by chromium treatment. Results obtained during IVGTT, which was conducted on days 0, 30, 50, and 70, suggested an increased insulin sensitivity and, consequently, a better utilization of glucose in the PoCr group. Lower basal concentrations of glucose and insulin in the PoCr group on days 30 and 70 were also obtained. Our results indicate that chromium supplementation improves glucose utilization in calves by enhancing insulin intracellular signaling in the skeletal muscle tissue.
PB  - Humana Press Inc, Totowa
T2  - Biological Trace Element Research
T1  - Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves
VL  - 180
IS  - 2
SP  - 223
EP  - 232
DO  - 10.1007/s12011-017-1007-1
ER  - 

@article{
author = "Jovanović, Ljubomir and Pantelić, Marija and Prodanović, Radiša and Vujanac, Ivan and Đurić, Miloje and Tepavcević, Snežana and Vranješ-Đurić, Sanja and Korićanac, Goran and Kirovski, Danijela",
year = "2017",
abstract = "The objective of this study was to investigate the effects of peroral administration of chromium-enriched yeast on glucose tolerance in Holstein calves, assessed by insulin signaling pathway molecule determination and intravenous glucose tolerance test (IVGTT). Twenty-four Holstein calves, aged 1 month, were chosen for the study and divided into two groups: the PoCr group (n = 12) that perorally received 0.04 mg of Cr/kg of body mass daily, for 70 days, and the NCr group (n = 12) that received no chromium supplementation. Skeletal tissue samples from each calf were obtained on day 0 and day 70 of the experiment. Chromium supplementation increased protein content of the insulin beta-subunit receptor, phosphorylation of insulin receptor substrate 1 at Tyrosine 632, phosphorylation of Akt at Serine 473, glucose transporter-4, and AMP-activated protein kinase in skeletal muscle tissue, while phosphorylation of insulin receptor substrate 1 at Serine 307 was not affected by chromium treatment. Results obtained during IVGTT, which was conducted on days 0, 30, 50, and 70, suggested an increased insulin sensitivity and, consequently, a better utilization of glucose in the PoCr group. Lower basal concentrations of glucose and insulin in the PoCr group on days 30 and 70 were also obtained. Our results indicate that chromium supplementation improves glucose utilization in calves by enhancing insulin intracellular signaling in the skeletal muscle tissue.",
publisher = "Humana Press Inc, Totowa",
journal = "Biological Trace Element Research",
title = "Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves",
volume = "180",
number = "2",
pages = "223-232",
doi = "10.1007/s12011-017-1007-1"
}

Jovanović, L., Pantelić, M., Prodanović, R., Vujanac, I., Đurić, M., Tepavcević, S., Vranješ-Đurić, S., Korićanac, G.,& Kirovski, D.. (2017). Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves. in Biological Trace Element Research
Humana Press Inc, Totowa., 180(2), 223-232.
https://doi.org/10.1007/s12011-017-1007-1

Jovanović L, Pantelić M, Prodanović R, Vujanac I, Đurić M, Tepavcević S, Vranješ-Đurić S, Korićanac G, Kirovski D. Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves. in Biological Trace Element Research. 2017;180(2):223-232.
doi:10.1007/s12011-017-1007-1 .

Jovanović, Ljubomir, Pantelić, Marija, Prodanović, Radiša, Vujanac, Ivan, Đurić, Miloje, Tepavcević, Snežana, Vranješ-Đurić, Sanja, Korićanac, Goran, Kirovski, Danijela, "Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves" in Biological Trace Element Research, 180, no. 2 (2017):223-232,
https://doi.org/10.1007/s12011-017-1007-1 . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Korićanac, Goran
AU  - Vujanac, Ivan
AU  - Đorđević, Ana
AU  - Pantelić, Marija
AU  - Romić, Snjezana
AU  - Stanimirović, Zoran
AU  - Kirovski, Danijela
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1396
AB  - We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their body condition score (BCS) as optimal (3.25 <= BCS <= 3.5) and high (4.0 <= BCS <= 425). Intravenous glucose tolerance test (GTT) and liver biopsies were carried out at day 10 before calving. Blood samples were collected before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels were determined at each sample point. In addition, beta-hydroxybutyrate and triglycerides levels were measured in the basal samples. The liver biopsies were analyzed for total lipid content and protein expression of insulin receptor beta (IR beta), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1). Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of the IR beta, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation.
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression
VL  - 107
SP  - 16
EP  - 19
DO  - 10.1016/j.rvsc.2016.04.007
ER  - 

@article{
author = "Prodanović, Radiša and Korićanac, Goran and Vujanac, Ivan and Đorđević, Ana and Pantelić, Marija and Romić, Snjezana and Stanimirović, Zoran and Kirovski, Danijela",
year = "2016",
abstract = "We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their body condition score (BCS) as optimal (3.25 <= BCS <= 3.5) and high (4.0 <= BCS <= 425). Intravenous glucose tolerance test (GTT) and liver biopsies were carried out at day 10 before calving. Blood samples were collected before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels were determined at each sample point. In addition, beta-hydroxybutyrate and triglycerides levels were measured in the basal samples. The liver biopsies were analyzed for total lipid content and protein expression of insulin receptor beta (IR beta), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1). Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of the IR beta, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression",
volume = "107",
pages = "16-19",
doi = "10.1016/j.rvsc.2016.04.007"
}

Prodanović, R., Korićanac, G., Vujanac, I., Đorđević, A., Pantelić, M., Romić, S., Stanimirović, Z.,& Kirovski, D.. (2016). Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 107, 16-19.
https://doi.org/10.1016/j.rvsc.2016.04.007

Prodanović R, Korićanac G, Vujanac I, Đorđević A, Pantelić M, Romić S, Stanimirović Z, Kirovski D. Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. in Research in Veterinary Science. 2016;107:16-19.
doi:10.1016/j.rvsc.2016.04.007 .

Prodanović, Radiša, Korićanac, Goran, Vujanac, Ivan, Đorđević, Ana, Pantelić, Marija, Romić, Snjezana, Stanimirović, Zoran, Kirovski, Danijela, "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression" in Research in Veterinary Science, 107 (2016):16-19,
https://doi.org/10.1016/j.rvsc.2016.04.007 . .