TY  - JOUR
AU  - Martacić, Jasmina
AU  - Kovačević-Filipović, Milica
AU  - Borozan, Sunčica
AU  - Cvetković, Zorica
AU  - Popović, Tamara
AU  - Arsić, Aleksandra
AU  - Takić, Marija
AU  - Vucić, Vesna
AU  - Glibetić, Maria
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1573
AB  - Objectives The aim of our study was to investigate whether N-acetyl-L-cysteine (NAC) could protect stem cells from exfoliated deciduous teeth (SHED) against oxidative damage, during in vitro cultivation, to preserve regenerative potential of these cells. Accordingly, we examined the potential of cell culture supplementation with NAC in prevention of lipid peroxidation, unfavorable changes of total lipids fatty acid composition, and the effects on the activity of antioxidant enzymes. Material and methods We analyzed the extent of oxidative damage in SHED after 48 h treatment with different NAC concentrations. Cellular lipid peroxidation was determined upon reaction with thiobarbituric acid. All enzyme activities were measured spectrophotometrically, based on published methods. Fatty acid methyl esters were analyzed by gas-liquid chromatography. Results Concentration of 0.1 mM NAC showed the most profound effects on SHED, significantly decreasing levels of lipid peroxidation in comparison to control. This dose also diminished the activities of antioxidant enzymes. Furthermore, NAC treatment significantly changed fatty acid composition of cells, reducing levels of oleic acid and monounsaturated fatty acids and increasing linoleic acid, n-6, and total polyunsaturated fatty acid (PUFA) proportions. Conclusion Low dose of NAC significantly decreased lipid peroxidation and altered fatty acid composition towards increasing PUFA. The reduced oxidative damage of cellular lipids could be strongly related to improved SHED survival in vitro.
PB  - Springer Heidelberg, Heidelberg
T2  - Clinical Oral Investigations
T1  - N-acetyl-L-cysteine protects dental tissue stem cells against oxidative stress in vitro
VL  - 22
IS  - 8
SP  - 2897
EP  - 2903
DO  - 10.1007/s00784-018-2377-2
ER  - 

@article{
author = "Martacić, Jasmina and Kovačević-Filipović, Milica and Borozan, Sunčica and Cvetković, Zorica and Popović, Tamara and Arsić, Aleksandra and Takić, Marija and Vucić, Vesna and Glibetić, Maria",
year = "2018",
abstract = "Objectives The aim of our study was to investigate whether N-acetyl-L-cysteine (NAC) could protect stem cells from exfoliated deciduous teeth (SHED) against oxidative damage, during in vitro cultivation, to preserve regenerative potential of these cells. Accordingly, we examined the potential of cell culture supplementation with NAC in prevention of lipid peroxidation, unfavorable changes of total lipids fatty acid composition, and the effects on the activity of antioxidant enzymes. Material and methods We analyzed the extent of oxidative damage in SHED after 48 h treatment with different NAC concentrations. Cellular lipid peroxidation was determined upon reaction with thiobarbituric acid. All enzyme activities were measured spectrophotometrically, based on published methods. Fatty acid methyl esters were analyzed by gas-liquid chromatography. Results Concentration of 0.1 mM NAC showed the most profound effects on SHED, significantly decreasing levels of lipid peroxidation in comparison to control. This dose also diminished the activities of antioxidant enzymes. Furthermore, NAC treatment significantly changed fatty acid composition of cells, reducing levels of oleic acid and monounsaturated fatty acids and increasing linoleic acid, n-6, and total polyunsaturated fatty acid (PUFA) proportions. Conclusion Low dose of NAC significantly decreased lipid peroxidation and altered fatty acid composition towards increasing PUFA. The reduced oxidative damage of cellular lipids could be strongly related to improved SHED survival in vitro.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Clinical Oral Investigations",
title = "N-acetyl-L-cysteine protects dental tissue stem cells against oxidative stress in vitro",
volume = "22",
number = "8",
pages = "2897-2903",
doi = "10.1007/s00784-018-2377-2"
}

Martacić, J., Kovačević-Filipović, M., Borozan, S., Cvetković, Z., Popović, T., Arsić, A., Takić, M., Vucić, V.,& Glibetić, M.. (2018). N-acetyl-L-cysteine protects dental tissue stem cells against oxidative stress in vitro. in Clinical Oral Investigations
Springer Heidelberg, Heidelberg., 22(8), 2897-2903.
https://doi.org/10.1007/s00784-018-2377-2

Martacić J, Kovačević-Filipović M, Borozan S, Cvetković Z, Popović T, Arsić A, Takić M, Vucić V, Glibetić M. N-acetyl-L-cysteine protects dental tissue stem cells against oxidative stress in vitro. in Clinical Oral Investigations. 2018;22(8):2897-2903.
doi:10.1007/s00784-018-2377-2 .

Martacić, Jasmina, Kovačević-Filipović, Milica, Borozan, Sunčica, Cvetković, Zorica, Popović, Tamara, Arsić, Aleksandra, Takić, Marija, Vucić, Vesna, Glibetić, Maria, "N-acetyl-L-cysteine protects dental tissue stem cells against oxidative stress in vitro" in Clinical Oral Investigations, 22, no. 8 (2018):2897-2903,
https://doi.org/10.1007/s00784-018-2377-2 . .

TY  - JOUR
AU  - Debeljak-Martačić, Jasmina
AU  - Borozan, Sunčica
AU  - Radovanović, Anita
AU  - Popadić, Dusan
AU  - Mojsilović, Slavko
AU  - Vucić, Vesna
AU  - Todorović, Vera
AU  - Kovačević-Filipović, Milica
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1385
AB  - Objective: Obtaining high number of stem cells is of interest for cell based therapies. N-Acetyl-L-cysteine (NAC) acts as a source of sulfhydryl groups and an anti-oxidative agent. The aim of this study was to test different NAC concentration on proliferation and differentiation of deciduous teeth dental pulp stem cells (DTSCs) in vitro as well as to define the possible underlining mechanism of its effect. Design: Number of viable, apoptotic and senescent DTSCs was determined after addition of NAC (0.1 mM, 1.0 mM, 2.0 mM). Also, cell cycle analysis, HIF1-alpha expression, LDH isoenzymes, superoxide-dismutase (SOD) and catalase (CAT) activity, sulfhydryl groups content, the level of lipids and proteins oxidative damage and differentiation capacity of NAC treated DTSCs was determined. Results: DTSCs expressed HIP-1 alpha in all conditions. The lowest NAC dose (0.1 mM) increased the number of DTSCs by one fifth comparing to the control, most likely stimulating entry of cells into S phase of cell cycle and enhancing the activity of LDH5 isoenzyme. The highest NAC dose (2 mM) inhibited DTSCs proliferation. Also, DTSCs had the lowest level of oxidative damage with 0.1 mM NAC. All tested NAC concentrations enhanced DTSCs osteo-chondrogenesis. Conclusion: The lowest NAC dose exerted significant positive effect on DTSCs proliferation as well as antioxidative protection creating beneficial environment for stem cells in vitro cultivation especially when their clinical use is important for stimulation of osteo-chondrogenesis.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Archives of Oral Biology
T1  - N-Acetyl-L-cysteine enhances ex-vivo amplification of deciduous teeth dental pulp stem cells
VL  - 70
SP  - 32
EP  - 38
DO  - 10.1016/j.archoralbio.2016.06.002
ER  - 

@article{
author = "Debeljak-Martačić, Jasmina and Borozan, Sunčica and Radovanović, Anita and Popadić, Dusan and Mojsilović, Slavko and Vucić, Vesna and Todorović, Vera and Kovačević-Filipović, Milica",
year = "2016",
abstract = "Objective: Obtaining high number of stem cells is of interest for cell based therapies. N-Acetyl-L-cysteine (NAC) acts as a source of sulfhydryl groups and an anti-oxidative agent. The aim of this study was to test different NAC concentration on proliferation and differentiation of deciduous teeth dental pulp stem cells (DTSCs) in vitro as well as to define the possible underlining mechanism of its effect. Design: Number of viable, apoptotic and senescent DTSCs was determined after addition of NAC (0.1 mM, 1.0 mM, 2.0 mM). Also, cell cycle analysis, HIF1-alpha expression, LDH isoenzymes, superoxide-dismutase (SOD) and catalase (CAT) activity, sulfhydryl groups content, the level of lipids and proteins oxidative damage and differentiation capacity of NAC treated DTSCs was determined. Results: DTSCs expressed HIP-1 alpha in all conditions. The lowest NAC dose (0.1 mM) increased the number of DTSCs by one fifth comparing to the control, most likely stimulating entry of cells into S phase of cell cycle and enhancing the activity of LDH5 isoenzyme. The highest NAC dose (2 mM) inhibited DTSCs proliferation. Also, DTSCs had the lowest level of oxidative damage with 0.1 mM NAC. All tested NAC concentrations enhanced DTSCs osteo-chondrogenesis. Conclusion: The lowest NAC dose exerted significant positive effect on DTSCs proliferation as well as antioxidative protection creating beneficial environment for stem cells in vitro cultivation especially when their clinical use is important for stimulation of osteo-chondrogenesis.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Archives of Oral Biology",
title = "N-Acetyl-L-cysteine enhances ex-vivo amplification of deciduous teeth dental pulp stem cells",
volume = "70",
pages = "32-38",
doi = "10.1016/j.archoralbio.2016.06.002"
}

Debeljak-Martačić, J., Borozan, S., Radovanović, A., Popadić, D., Mojsilović, S., Vucić, V., Todorović, V.,& Kovačević-Filipović, M.. (2016). N-Acetyl-L-cysteine enhances ex-vivo amplification of deciduous teeth dental pulp stem cells. in Archives of Oral Biology
Pergamon-Elsevier Science Ltd, Oxford., 70, 32-38.
https://doi.org/10.1016/j.archoralbio.2016.06.002

Debeljak-Martačić J, Borozan S, Radovanović A, Popadić D, Mojsilović S, Vucić V, Todorović V, Kovačević-Filipović M. N-Acetyl-L-cysteine enhances ex-vivo amplification of deciduous teeth dental pulp stem cells. in Archives of Oral Biology. 2016;70:32-38.
doi:10.1016/j.archoralbio.2016.06.002 .

Debeljak-Martačić, Jasmina, Borozan, Sunčica, Radovanović, Anita, Popadić, Dusan, Mojsilović, Slavko, Vucić, Vesna, Todorović, Vera, Kovačević-Filipović, Milica, "N-Acetyl-L-cysteine enhances ex-vivo amplification of deciduous teeth dental pulp stem cells" in Archives of Oral Biology, 70 (2016):32-38,
https://doi.org/10.1016/j.archoralbio.2016.06.002 . .

TY  - JOUR
AU  - Arsić, Aleksandra
AU  - Vucić, Vesna
AU  - Glibetić, Marija
AU  - Popović, Tamara
AU  - Debeljak-Martačić, Jasmina
AU  - Cubrilo, Dejan
AU  - Ahmetović, Zlatko
AU  - Perić, Dusan
AU  - Borozan, Sunčica
AU  - Đurić, Dragan
AU  - Barudzić, Nevena
AU  - Jakovljević, Vladimir
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1427
AB  - BACKGROUND: The aim of the present study was to analyze changes in redox balance throughout parameters of oxidative stress and activities of antioxidant enzymes in elite female water polo (N.=15) and football players (N.=19) aged between 20 and 23. Fourteen age-matched sedentary women were also included in the study. METHODS: Blood sampling was performed to measure levels of lipid peroxidation (MDA), total antioxidant status (TAS), superoxide anion radical (O2), hydrogen peroxide (H2O2), reduced glutathione (GSH), oxidized glutathione (GSSG), nitrites, superoxide dismutase activity (SOD), catalase activity (CAT) and glutathione-peroxidase activity (GPx). RESULTS: Levels of MDA, TAS, GSSG and H2O2 were significantly higher in athletes than in the control women. Football players had higher levels of O2- than the other two groups. Activity of SOD was higher in water polo players when compared with the football and control groups, CAT was increased in all athletes, while GPx did not differ among groups. CONCLUSIONS: Therefore, prolonged intensive training markedly increases oxidative stress in women, which depends on the type of sport. Lower concentration of O2- and increased activity of SOD in water polo players compared to football players suggest that mechanisms of adaptation of antioxidative defense are related to the type of exercise.
PB  - Edizioni Minerva Medica, Turin
T2  - Journal of Sports Medicine and Physical Fitness
T1  - Redox balance in elite female athletes: Differences based on sport types
VL  - 56
IS  - 1-2
SP  - 1
EP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1427
ER  - 

@article{
author = "Arsić, Aleksandra and Vucić, Vesna and Glibetić, Marija and Popović, Tamara and Debeljak-Martačić, Jasmina and Cubrilo, Dejan and Ahmetović, Zlatko and Perić, Dusan and Borozan, Sunčica and Đurić, Dragan and Barudzić, Nevena and Jakovljević, Vladimir",
year = "2016",
abstract = "BACKGROUND: The aim of the present study was to analyze changes in redox balance throughout parameters of oxidative stress and activities of antioxidant enzymes in elite female water polo (N.=15) and football players (N.=19) aged between 20 and 23. Fourteen age-matched sedentary women were also included in the study. METHODS: Blood sampling was performed to measure levels of lipid peroxidation (MDA), total antioxidant status (TAS), superoxide anion radical (O2), hydrogen peroxide (H2O2), reduced glutathione (GSH), oxidized glutathione (GSSG), nitrites, superoxide dismutase activity (SOD), catalase activity (CAT) and glutathione-peroxidase activity (GPx). RESULTS: Levels of MDA, TAS, GSSG and H2O2 were significantly higher in athletes than in the control women. Football players had higher levels of O2- than the other two groups. Activity of SOD was higher in water polo players when compared with the football and control groups, CAT was increased in all athletes, while GPx did not differ among groups. CONCLUSIONS: Therefore, prolonged intensive training markedly increases oxidative stress in women, which depends on the type of sport. Lower concentration of O2- and increased activity of SOD in water polo players compared to football players suggest that mechanisms of adaptation of antioxidative defense are related to the type of exercise.",
publisher = "Edizioni Minerva Medica, Turin",
journal = "Journal of Sports Medicine and Physical Fitness",
title = "Redox balance in elite female athletes: Differences based on sport types",
volume = "56",
number = "1-2",
pages = "1-8",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1427"
}

Arsić, A., Vucić, V., Glibetić, M., Popović, T., Debeljak-Martačić, J., Cubrilo, D., Ahmetović, Z., Perić, D., Borozan, S., Đurić, D., Barudzić, N.,& Jakovljević, V.. (2016). Redox balance in elite female athletes: Differences based on sport types. in Journal of Sports Medicine and Physical Fitness
Edizioni Minerva Medica, Turin., 56(1-2), 1-8.
https://hdl.handle.net/21.15107/rcub_veterinar_1427

Arsić A, Vucić V, Glibetić M, Popović T, Debeljak-Martačić J, Cubrilo D, Ahmetović Z, Perić D, Borozan S, Đurić D, Barudzić N, Jakovljević V. Redox balance in elite female athletes: Differences based on sport types. in Journal of Sports Medicine and Physical Fitness. 2016;56(1-2):1-8.
https://hdl.handle.net/21.15107/rcub_veterinar_1427 .

Arsić, Aleksandra, Vucić, Vesna, Glibetić, Marija, Popović, Tamara, Debeljak-Martačić, Jasmina, Cubrilo, Dejan, Ahmetović, Zlatko, Perić, Dusan, Borozan, Sunčica, Đurić, Dragan, Barudzić, Nevena, Jakovljević, Vladimir, "Redox balance in elite female athletes: Differences based on sport types" in Journal of Sports Medicine and Physical Fitness, 56, no. 1-2 (2016):1-8,
https://hdl.handle.net/21.15107/rcub_veterinar_1427 .

TY  - JOUR
AU  - Popović, Tamara
AU  - Borozan, Sunčica
AU  - Takić, Marija M.
AU  - Kojadinović, Milica J.
AU  - Ranković, Slavica
AU  - Ranić, Marija
AU  - de Luka, Silvio
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1095
AB  - Fish oil affects oxidative stress parameters and changes in phospholipids fatty acids profiles in plasma, erythrocytes and tissues. We examined the effects of fish oil supplementation in young and old male Wistar rats (3 and 22 months old) on plasma phospholipids fatty acids profiles and blood oxidative stress parameters. Twenty young and twenty aged Wistar rats were randomly divided into four groups (ten animals each): two control groups and two supplemented groups treated for 6 weeks with fish oil capsules containing 45 mg eicosapentanoic acid (EPA) and 30 mg docosahexanoic acid (DHA). Fish oil supplementation changed the percentage of long chain fatty acids (FAs): the elevated percentage of eicosatrienoic acid (ETA, 20:3), eicosapentanoic (EPA, 20:5), docosapentanoic acid (DPA, 22:5), n-3 fatty acids and decreased arachidonic acid (AA, 20:4). However, there were no age-related changes in total SH groups, the percentages of palmitic (16:0), palmitoleic (16:1), oleic (18:1 (n-9)) and linoleic acid (18:2) in plasma phospholipids and MUFA and they were neither reversed nor prevented by fish oil supplementation. Results showed that fish oil supplementation increased SOD and CAT activities in erythrocytes, and PON activity in the blood plasma of both young and aged rats. Furthermore, fish oil supplementation decreased lipid peroxidation (MDA) and nitrite levels in both young and aged rats implying better antioxidant protection and a lower level of oxidative pressure after fish oil supplementation. Our results suggest that fish oil supplementation is beneficial regarding better antioxidant protection in both young and aged rats, while applied treatment differs in plasma phospholipids FAs composition.
PB  - Croatian Chemical Soc, Zagreb
T2  - Croatica Chemica Acta
T1  - Fatty Acid Composition and Oxidative Stress Parameters in Plasma after Fish Oil Supplementation in Aging
VL  - 87
IS  - 3
SP  - 207
EP  - 212
DO  - 10.5562/cca2405
ER  - 

@article{
author = "Popović, Tamara and Borozan, Sunčica and Takić, Marija M. and Kojadinović, Milica J. and Ranković, Slavica and Ranić, Marija and de Luka, Silvio",
year = "2014",
abstract = "Fish oil affects oxidative stress parameters and changes in phospholipids fatty acids profiles in plasma, erythrocytes and tissues. We examined the effects of fish oil supplementation in young and old male Wistar rats (3 and 22 months old) on plasma phospholipids fatty acids profiles and blood oxidative stress parameters. Twenty young and twenty aged Wistar rats were randomly divided into four groups (ten animals each): two control groups and two supplemented groups treated for 6 weeks with fish oil capsules containing 45 mg eicosapentanoic acid (EPA) and 30 mg docosahexanoic acid (DHA). Fish oil supplementation changed the percentage of long chain fatty acids (FAs): the elevated percentage of eicosatrienoic acid (ETA, 20:3), eicosapentanoic (EPA, 20:5), docosapentanoic acid (DPA, 22:5), n-3 fatty acids and decreased arachidonic acid (AA, 20:4). However, there were no age-related changes in total SH groups, the percentages of palmitic (16:0), palmitoleic (16:1), oleic (18:1 (n-9)) and linoleic acid (18:2) in plasma phospholipids and MUFA and they were neither reversed nor prevented by fish oil supplementation. Results showed that fish oil supplementation increased SOD and CAT activities in erythrocytes, and PON activity in the blood plasma of both young and aged rats. Furthermore, fish oil supplementation decreased lipid peroxidation (MDA) and nitrite levels in both young and aged rats implying better antioxidant protection and a lower level of oxidative pressure after fish oil supplementation. Our results suggest that fish oil supplementation is beneficial regarding better antioxidant protection in both young and aged rats, while applied treatment differs in plasma phospholipids FAs composition.",
publisher = "Croatian Chemical Soc, Zagreb",
journal = "Croatica Chemica Acta",
title = "Fatty Acid Composition and Oxidative Stress Parameters in Plasma after Fish Oil Supplementation in Aging",
volume = "87",
number = "3",
pages = "207-212",
doi = "10.5562/cca2405"
}

Popović, T., Borozan, S., Takić, M. M., Kojadinović, M. J., Ranković, S., Ranić, M.,& de Luka, S.. (2014). Fatty Acid Composition and Oxidative Stress Parameters in Plasma after Fish Oil Supplementation in Aging. in Croatica Chemica Acta
Croatian Chemical Soc, Zagreb., 87(3), 207-212.
https://doi.org/10.5562/cca2405

Popović T, Borozan S, Takić MM, Kojadinović MJ, Ranković S, Ranić M, de Luka S. Fatty Acid Composition and Oxidative Stress Parameters in Plasma after Fish Oil Supplementation in Aging. in Croatica Chemica Acta. 2014;87(3):207-212.
doi:10.5562/cca2405 .

Popović, Tamara, Borozan, Sunčica, Takić, Marija M., Kojadinović, Milica J., Ranković, Slavica, Ranić, Marija, de Luka, Silvio, "Fatty Acid Composition and Oxidative Stress Parameters in Plasma after Fish Oil Supplementation in Aging" in Croatica Chemica Acta, 87, no. 3 (2014):207-212,
https://doi.org/10.5562/cca2405 . .

TY  - JOUR
AU  - Rasic-Milutinović, Zorica
AU  - Popović, Tamara
AU  - Peruničić-Peković, Gordana
AU  - Arsić, Aleksandra
AU  - Borozan, Sunčica
AU  - Glibetić, Marija
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/878
AB  - Background. Serum paraoxonase-1(PON-1) activity is decreased in clinical conditions associated with low high-density lipoprotein cholesterol (HDL-C), increased lipid peroxidation and low-grade chronic inflammation, as in type 2 diabetes mellitus (T2DM). Until now there are no data about the association of any fatty acid (FA) with PON-1 activity in T2DM. Methods. Twenty patients with T2DM and 16 healthy controls were included in this cross-sectional study. Serum PON-1 activity, superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activity as well as plasma glucose, HbA1c, lipids, high-sensitivity C-reactive protein (hs-CRP) and insulin resistance, homeostasis model assessment (HOMA-IR) were measured. The preparation of FA methyl esters and their gas chromatography (GC) analysis were also performed. Results. HbA1c, plasma insulin, HOMA-IR and triglycerides were higher in patients with T2DM, whereas HDL-C was lower in those subjects. Levels of pro-oxidative enzyme malondialdehyde (MDA) and hs-CRP were significantly higher, and antioxidative enzymes SOD and PON-1 activity were decreased in T2DM patients. N-6 PUFAs were higher in T2DM patients, particularly linoleic acid (LA, 18:2 n-6) and arachidonic acid (AA, 20:4 n-6), whereas n-3 PUFA, docosahexaenoic acid (DHA, 22:6 n-3) was lower in T2DM patients. Using regression analysis, we have shown that only LA and DHA independently predicted PON-1 activity of all participants, particularly in patients with T2DM. Conclusions. Decreased serum PON-1 activity may, in part, be influenced by higher levels of LA and lower levels of DHA in patients with 12DM. Prospective, randomized studies are necessary to confirm these preliminary findings.
PB  - Elsevier Science Inc, New York
T2  - Archives of Medical Research
T1  - Lower Serum Paraoxonase-1 Activity Is Related to Linoleic and Docosahexanoic Fatty Acids in Type 2 Diabetic Patients
VL  - 43
IS  - 1
SP  - 75
EP  - 82
DO  - 10.1016/j.arcmed.2011.12.008
ER  - 

@article{
author = "Rasic-Milutinović, Zorica and Popović, Tamara and Peruničić-Peković, Gordana and Arsić, Aleksandra and Borozan, Sunčica and Glibetić, Marija",
year = "2012",
abstract = "Background. Serum paraoxonase-1(PON-1) activity is decreased in clinical conditions associated with low high-density lipoprotein cholesterol (HDL-C), increased lipid peroxidation and low-grade chronic inflammation, as in type 2 diabetes mellitus (T2DM). Until now there are no data about the association of any fatty acid (FA) with PON-1 activity in T2DM. Methods. Twenty patients with T2DM and 16 healthy controls were included in this cross-sectional study. Serum PON-1 activity, superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activity as well as plasma glucose, HbA1c, lipids, high-sensitivity C-reactive protein (hs-CRP) and insulin resistance, homeostasis model assessment (HOMA-IR) were measured. The preparation of FA methyl esters and their gas chromatography (GC) analysis were also performed. Results. HbA1c, plasma insulin, HOMA-IR and triglycerides were higher in patients with T2DM, whereas HDL-C was lower in those subjects. Levels of pro-oxidative enzyme malondialdehyde (MDA) and hs-CRP were significantly higher, and antioxidative enzymes SOD and PON-1 activity were decreased in T2DM patients. N-6 PUFAs were higher in T2DM patients, particularly linoleic acid (LA, 18:2 n-6) and arachidonic acid (AA, 20:4 n-6), whereas n-3 PUFA, docosahexaenoic acid (DHA, 22:6 n-3) was lower in T2DM patients. Using regression analysis, we have shown that only LA and DHA independently predicted PON-1 activity of all participants, particularly in patients with T2DM. Conclusions. Decreased serum PON-1 activity may, in part, be influenced by higher levels of LA and lower levels of DHA in patients with 12DM. Prospective, randomized studies are necessary to confirm these preliminary findings.",
publisher = "Elsevier Science Inc, New York",
journal = "Archives of Medical Research",
title = "Lower Serum Paraoxonase-1 Activity Is Related to Linoleic and Docosahexanoic Fatty Acids in Type 2 Diabetic Patients",
volume = "43",
number = "1",
pages = "75-82",
doi = "10.1016/j.arcmed.2011.12.008"
}

Rasic-Milutinović, Z., Popović, T., Peruničić-Peković, G., Arsić, A., Borozan, S.,& Glibetić, M.. (2012). Lower Serum Paraoxonase-1 Activity Is Related to Linoleic and Docosahexanoic Fatty Acids in Type 2 Diabetic Patients. in Archives of Medical Research
Elsevier Science Inc, New York., 43(1), 75-82.
https://doi.org/10.1016/j.arcmed.2011.12.008

Rasic-Milutinović Z, Popović T, Peruničić-Peković G, Arsić A, Borozan S, Glibetić M. Lower Serum Paraoxonase-1 Activity Is Related to Linoleic and Docosahexanoic Fatty Acids in Type 2 Diabetic Patients. in Archives of Medical Research. 2012;43(1):75-82.
doi:10.1016/j.arcmed.2011.12.008 .

Rasic-Milutinović, Zorica, Popović, Tamara, Peruničić-Peković, Gordana, Arsić, Aleksandra, Borozan, Sunčica, Glibetić, Marija, "Lower Serum Paraoxonase-1 Activity Is Related to Linoleic and Docosahexanoic Fatty Acids in Type 2 Diabetic Patients" in Archives of Medical Research, 43, no. 1 (2012):75-82,
https://doi.org/10.1016/j.arcmed.2011.12.008 . .

TY  - JOUR
AU  - Popović, Tamara
AU  - Borozan, Sunčica
AU  - Arsić, Aleksandra
AU  - Martacić, J. D.
AU  - Vucić, V.
AU  - Trbović, A.
AU  - Mandić, L.
AU  - Glibetić, Maria
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/865
AB  - In the present study, we examined the effects of fish oil supplementation in 3 months old male Wistar rats on changes in plasma and liver lipid metabolism and oxidative stress parameters. Twenty Wistar rats were randomly divided into two groups of ten animals: control group and intervention group, treated for 6 weeks with fish oil capsules containing 45 mg eicosapentanoic acid and 30 mg docosahexanoic acid. After intervention, biochemical parameters in plasma [triglycerides (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL) and total cholesterol, urea, creatinine and uric acid], fatty acid (FAs) profile of liver phospholipids and parameters of oxidative stress in liver [activity of catalase, superoxide dismutase and paraoxonase (PON1), concentration of nitrites, lipid peroxidation (LPO), free thiol (SH) groups and lactate dehydrogenase (LDH) izoenzymes were determined. Treatment with fish oil improved FAs profile of liver phospholipids, increasing n-3 FAs and decreasing n-6/n-3 ratio. Significant decrease in plasma TG and LDL concentration, and increase in the level of HDL and uric acid were found in intervention group at the end of the study. Catalase activity, LPO, and nitrites concentration in liver were significantly decreased, after the supplementation, together with elevated PON1 activity. Applied treatment significantly improved plasma lipid profile, liver FAs composition and parameters of oxidative stress in male Wistar rats.
PB  - Wiley, Hoboken
T2  - Journal of Animal Physiology and Animal Nutrition
T1  - Fish oil supplementation improved liver phospholipids fatty acid composition and parameters of oxidative stress in male wistar rats
VL  - 96
IS  - 6
SP  - 1020
EP  - 1029
DO  - 10.1111/j.1439-0396.2011.01216.x
ER  - 

@article{
author = "Popović, Tamara and Borozan, Sunčica and Arsić, Aleksandra and Martacić, J. D. and Vucić, V. and Trbović, A. and Mandić, L. and Glibetić, Maria",
year = "2012",
abstract = "In the present study, we examined the effects of fish oil supplementation in 3 months old male Wistar rats on changes in plasma and liver lipid metabolism and oxidative stress parameters. Twenty Wistar rats were randomly divided into two groups of ten animals: control group and intervention group, treated for 6 weeks with fish oil capsules containing 45 mg eicosapentanoic acid and 30 mg docosahexanoic acid. After intervention, biochemical parameters in plasma [triglycerides (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL) and total cholesterol, urea, creatinine and uric acid], fatty acid (FAs) profile of liver phospholipids and parameters of oxidative stress in liver [activity of catalase, superoxide dismutase and paraoxonase (PON1), concentration of nitrites, lipid peroxidation (LPO), free thiol (SH) groups and lactate dehydrogenase (LDH) izoenzymes were determined. Treatment with fish oil improved FAs profile of liver phospholipids, increasing n-3 FAs and decreasing n-6/n-3 ratio. Significant decrease in plasma TG and LDL concentration, and increase in the level of HDL and uric acid were found in intervention group at the end of the study. Catalase activity, LPO, and nitrites concentration in liver were significantly decreased, after the supplementation, together with elevated PON1 activity. Applied treatment significantly improved plasma lipid profile, liver FAs composition and parameters of oxidative stress in male Wistar rats.",
publisher = "Wiley, Hoboken",
journal = "Journal of Animal Physiology and Animal Nutrition",
title = "Fish oil supplementation improved liver phospholipids fatty acid composition and parameters of oxidative stress in male wistar rats",
volume = "96",
number = "6",
pages = "1020-1029",
doi = "10.1111/j.1439-0396.2011.01216.x"
}

Popović, T., Borozan, S., Arsić, A., Martacić, J. D., Vucić, V., Trbović, A., Mandić, L.,& Glibetić, M.. (2012). Fish oil supplementation improved liver phospholipids fatty acid composition and parameters of oxidative stress in male wistar rats. in Journal of Animal Physiology and Animal Nutrition
Wiley, Hoboken., 96(6), 1020-1029.
https://doi.org/10.1111/j.1439-0396.2011.01216.x

Popović T, Borozan S, Arsić A, Martacić JD, Vucić V, Trbović A, Mandić L, Glibetić M. Fish oil supplementation improved liver phospholipids fatty acid composition and parameters of oxidative stress in male wistar rats. in Journal of Animal Physiology and Animal Nutrition. 2012;96(6):1020-1029.
doi:10.1111/j.1439-0396.2011.01216.x .

Popović, Tamara, Borozan, Sunčica, Arsić, Aleksandra, Martacić, J. D., Vucić, V., Trbović, A., Mandić, L., Glibetić, Maria, "Fish oil supplementation improved liver phospholipids fatty acid composition and parameters of oxidative stress in male wistar rats" in Journal of Animal Physiology and Animal Nutrition, 96, no. 6 (2012):1020-1029,
https://doi.org/10.1111/j.1439-0396.2011.01216.x . .

TY  - JOUR
AU  - Popović, Tamara
AU  - Borozan, Sunčica
AU  - Arsić, Aleksandra
AU  - Debeljak-Martačić, Jasmina
AU  - Vucić, Vesna
AU  - de Luka, Silvio
AU  - Milovanović, Ivan
AU  - Trbović, Aleksandar
AU  - Glibetić, Maria
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/799
AB  - The effects of fish oil supplementation in Wistar rats are focused on cardiovascular, endocrine, metabolic and antioxidant status changes. We determined plasma and liver phospholipid fatty acids (FAs) status and plasma lipid concentrations in aged Wistar rats. Our results showed differences in plasma and liver FAs profiles as well as plasma chlolesterol (CHOL), triglicerides (TG), high density lipoproteins (HDL), low density lipoproteins (LDL), CHOL/HDL ratio (risk factor for atherosclerosis) and LDL/HDL ratio (risk for cardiovascular diseases) between treated and control group of animals. In fish oil treated group there were statistically significant changes in FAs profile in increasing linoleic acid (LA), dihomo-gamma linoleic acid, eicopentanoic acid (EPA) and docosapentaenoic acid (DPA) and decreasing in arachidonic acid (AA) concentration. Also, liver phospholipids FAs results showed increasing concentrations of vascenic acid, LA, EPA, and DPA and decreasing concentration of AA after supplementation of fish oil compared to control group. However, concentrations of CHOL, LDL and non HDL concentrations decreased while HDL increased in fish oil group. CHOL/HDL, LDL/HDL ratios decreased. These findings suggest that long term treatment of fish oil in aged Wistar rats can be beneficial in decreasing LDL, and decreasing risk factors for developing atherosclerosis and cardiovascular diseases. (doi: 10.5562/cca1751)
PB  - Croatian Chemical Soc, Zagreb
T2  - Croatica Chemica Acta
T1  - Effects of n-3 Supplementation on Plasma and Liver Phospholipid Fatty Acids Profile in Aged Wistar Rats
VL  - 84
IS  - 1
SP  - 73
EP  - 79
DO  - 10.5562/cca1751
ER  - 

@article{
author = "Popović, Tamara and Borozan, Sunčica and Arsić, Aleksandra and Debeljak-Martačić, Jasmina and Vucić, Vesna and de Luka, Silvio and Milovanović, Ivan and Trbović, Aleksandar and Glibetić, Maria",
year = "2011",
abstract = "The effects of fish oil supplementation in Wistar rats are focused on cardiovascular, endocrine, metabolic and antioxidant status changes. We determined plasma and liver phospholipid fatty acids (FAs) status and plasma lipid concentrations in aged Wistar rats. Our results showed differences in plasma and liver FAs profiles as well as plasma chlolesterol (CHOL), triglicerides (TG), high density lipoproteins (HDL), low density lipoproteins (LDL), CHOL/HDL ratio (risk factor for atherosclerosis) and LDL/HDL ratio (risk for cardiovascular diseases) between treated and control group of animals. In fish oil treated group there were statistically significant changes in FAs profile in increasing linoleic acid (LA), dihomo-gamma linoleic acid, eicopentanoic acid (EPA) and docosapentaenoic acid (DPA) and decreasing in arachidonic acid (AA) concentration. Also, liver phospholipids FAs results showed increasing concentrations of vascenic acid, LA, EPA, and DPA and decreasing concentration of AA after supplementation of fish oil compared to control group. However, concentrations of CHOL, LDL and non HDL concentrations decreased while HDL increased in fish oil group. CHOL/HDL, LDL/HDL ratios decreased. These findings suggest that long term treatment of fish oil in aged Wistar rats can be beneficial in decreasing LDL, and decreasing risk factors for developing atherosclerosis and cardiovascular diseases. (doi: 10.5562/cca1751)",
publisher = "Croatian Chemical Soc, Zagreb",
journal = "Croatica Chemica Acta",
title = "Effects of n-3 Supplementation on Plasma and Liver Phospholipid Fatty Acids Profile in Aged Wistar Rats",
volume = "84",
number = "1",
pages = "73-79",
doi = "10.5562/cca1751"
}

Popović, T., Borozan, S., Arsić, A., Debeljak-Martačić, J., Vucić, V., de Luka, S., Milovanović, I., Trbović, A.,& Glibetić, M.. (2011). Effects of n-3 Supplementation on Plasma and Liver Phospholipid Fatty Acids Profile in Aged Wistar Rats. in Croatica Chemica Acta
Croatian Chemical Soc, Zagreb., 84(1), 73-79.
https://doi.org/10.5562/cca1751

Popović T, Borozan S, Arsić A, Debeljak-Martačić J, Vucić V, de Luka S, Milovanović I, Trbović A, Glibetić M. Effects of n-3 Supplementation on Plasma and Liver Phospholipid Fatty Acids Profile in Aged Wistar Rats. in Croatica Chemica Acta. 2011;84(1):73-79.
doi:10.5562/cca1751 .

Popović, Tamara, Borozan, Sunčica, Arsić, Aleksandra, Debeljak-Martačić, Jasmina, Vucić, Vesna, de Luka, Silvio, Milovanović, Ivan, Trbović, Aleksandar, Glibetić, Maria, "Effects of n-3 Supplementation on Plasma and Liver Phospholipid Fatty Acids Profile in Aged Wistar Rats" in Croatica Chemica Acta, 84, no. 1 (2011):73-79,
https://doi.org/10.5562/cca1751 . .