TY  - JOUR
AU  - Ravić, Branko
AU  - Debeljak-Martacić, Jasmina
AU  - Pokimica, Biljana
AU  - Vidović, Nevena
AU  - Ranković, Slavica
AU  - Glibetić, Marija
AU  - Stepanović, Predrag
AU  - Popović, Tamara
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2834
AB  - The synthesis, degradation, and reconstruction of the cell membrane as a metabolic pathway
of phospholipids is a constant and dynamic process. Fatty acids as bioactive lipid components of
plasma and erythrocyte phospholipids as structural lipids have biological roles in the integrity of
cell membranes. Fatty acids, depending on the chain length, the degree of saturation, and the
synthesis pathways, can alleviate inflammation and oxidative stress caused by excessive exercise.
Considering that changing food intake or diet can influence fatty acid phospholipid metabolism,
our study aimed to determine the potential benefits of fish-based diets in working (police) dogs
undergoing intensive training concerning bioactive lipids such as fatty acids, phospholipids of
plasma, and erythrocytes. Fatty acid esters’ composition of plasma and erythrocyte phospholipids
as a bioactive lipids, in addition to markers of oxidative stress and metabolic parameters, were
analysed by GC chromatography. The food was well tolerated by all dogs, and the compliance to the
diet was high throughout the study. After the treatment with fish-based food, blood glucose, total,
and LDL cholesterol levels were significantly reduced, indicating positive biochemical profiles of
dogs. Correlations of fatty acid phospholipid compositions between plasma and erythrocytes have
shown that both plasma and erythrocytes could represent markers of omega-3 eicosapentaenoic and
docosahexaenoic acid intake levels in dogs. Morover, fish-based food supplementation caused a
significant reduction in lipid peroxidation markers. The enrichment of dogs’ diets with marine fish
could improve oxidative status and improve roles and status of bioactive lipids, such as membrane
phospholipids and fatty acids, as its components in plasma and erythrocytes in police dogs exposed to intensive exercise.
PB  - MDPI
T2  - Biomolecules
T1  - The Effect of Fish Oil-Based Foods on Lipid and Oxidative Status Parameters in Police Dogs
VL  - 12
IS  - 8
SP  - 1092
DO  - 10.3390/biom12081092
ER  - 

@article{
author = "Ravić, Branko and Debeljak-Martacić, Jasmina and Pokimica, Biljana and Vidović, Nevena and Ranković, Slavica and Glibetić, Marija and Stepanović, Predrag and Popović, Tamara",
year = "2022",
abstract = "The synthesis, degradation, and reconstruction of the cell membrane as a metabolic pathway
of phospholipids is a constant and dynamic process. Fatty acids as bioactive lipid components of
plasma and erythrocyte phospholipids as structural lipids have biological roles in the integrity of
cell membranes. Fatty acids, depending on the chain length, the degree of saturation, and the
synthesis pathways, can alleviate inflammation and oxidative stress caused by excessive exercise.
Considering that changing food intake or diet can influence fatty acid phospholipid metabolism,
our study aimed to determine the potential benefits of fish-based diets in working (police) dogs
undergoing intensive training concerning bioactive lipids such as fatty acids, phospholipids of
plasma, and erythrocytes. Fatty acid esters’ composition of plasma and erythrocyte phospholipids
as a bioactive lipids, in addition to markers of oxidative stress and metabolic parameters, were
analysed by GC chromatography. The food was well tolerated by all dogs, and the compliance to the
diet was high throughout the study. After the treatment with fish-based food, blood glucose, total,
and LDL cholesterol levels were significantly reduced, indicating positive biochemical profiles of
dogs. Correlations of fatty acid phospholipid compositions between plasma and erythrocytes have
shown that both plasma and erythrocytes could represent markers of omega-3 eicosapentaenoic and
docosahexaenoic acid intake levels in dogs. Morover, fish-based food supplementation caused a
significant reduction in lipid peroxidation markers. The enrichment of dogs’ diets with marine fish
could improve oxidative status and improve roles and status of bioactive lipids, such as membrane
phospholipids and fatty acids, as its components in plasma and erythrocytes in police dogs exposed to intensive exercise.",
publisher = "MDPI",
journal = "Biomolecules",
title = "The Effect of Fish Oil-Based Foods on Lipid and Oxidative Status Parameters in Police Dogs",
volume = "12",
number = "8",
pages = "1092",
doi = "10.3390/biom12081092"
}

Ravić, B., Debeljak-Martacić, J., Pokimica, B., Vidović, N., Ranković, S., Glibetić, M., Stepanović, P.,& Popović, T.. (2022). The Effect of Fish Oil-Based Foods on Lipid and Oxidative Status Parameters in Police Dogs. in Biomolecules
MDPI., 12(8), 1092.
https://doi.org/10.3390/biom12081092

Ravić B, Debeljak-Martacić J, Pokimica B, Vidović N, Ranković S, Glibetić M, Stepanović P, Popović T. The Effect of Fish Oil-Based Foods on Lipid and Oxidative Status Parameters in Police Dogs. in Biomolecules. 2022;12(8):1092.
doi:10.3390/biom12081092 .

Ravić, Branko, Debeljak-Martacić, Jasmina, Pokimica, Biljana, Vidović, Nevena, Ranković, Slavica, Glibetić, Marija, Stepanović, Predrag, Popović, Tamara, "The Effect of Fish Oil-Based Foods on Lipid and Oxidative Status Parameters in Police Dogs" in Biomolecules, 12, no. 8 (2022):1092,
https://doi.org/10.3390/biom12081092 . .

TY  - JOUR
AU  - Arsić, Aleksandra
AU  - Vucić, Vesna
AU  - Glibetić, Marija
AU  - Popović, Tamara
AU  - Debeljak-Martačić, Jasmina
AU  - Cubrilo, Dejan
AU  - Ahmetović, Zlatko
AU  - Perić, Dusan
AU  - Borozan, Sunčica
AU  - Đurić, Dragan
AU  - Barudzić, Nevena
AU  - Jakovljević, Vladimir
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1427
AB  - BACKGROUND: The aim of the present study was to analyze changes in redox balance throughout parameters of oxidative stress and activities of antioxidant enzymes in elite female water polo (N.=15) and football players (N.=19) aged between 20 and 23. Fourteen age-matched sedentary women were also included in the study. METHODS: Blood sampling was performed to measure levels of lipid peroxidation (MDA), total antioxidant status (TAS), superoxide anion radical (O2), hydrogen peroxide (H2O2), reduced glutathione (GSH), oxidized glutathione (GSSG), nitrites, superoxide dismutase activity (SOD), catalase activity (CAT) and glutathione-peroxidase activity (GPx). RESULTS: Levels of MDA, TAS, GSSG and H2O2 were significantly higher in athletes than in the control women. Football players had higher levels of O2- than the other two groups. Activity of SOD was higher in water polo players when compared with the football and control groups, CAT was increased in all athletes, while GPx did not differ among groups. CONCLUSIONS: Therefore, prolonged intensive training markedly increases oxidative stress in women, which depends on the type of sport. Lower concentration of O2- and increased activity of SOD in water polo players compared to football players suggest that mechanisms of adaptation of antioxidative defense are related to the type of exercise.
PB  - Edizioni Minerva Medica, Turin
T2  - Journal of Sports Medicine and Physical Fitness
T1  - Redox balance in elite female athletes: Differences based on sport types
VL  - 56
IS  - 1-2
SP  - 1
EP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1427
ER  - 

@article{
author = "Arsić, Aleksandra and Vucić, Vesna and Glibetić, Marija and Popović, Tamara and Debeljak-Martačić, Jasmina and Cubrilo, Dejan and Ahmetović, Zlatko and Perić, Dusan and Borozan, Sunčica and Đurić, Dragan and Barudzić, Nevena and Jakovljević, Vladimir",
year = "2016",
abstract = "BACKGROUND: The aim of the present study was to analyze changes in redox balance throughout parameters of oxidative stress and activities of antioxidant enzymes in elite female water polo (N.=15) and football players (N.=19) aged between 20 and 23. Fourteen age-matched sedentary women were also included in the study. METHODS: Blood sampling was performed to measure levels of lipid peroxidation (MDA), total antioxidant status (TAS), superoxide anion radical (O2), hydrogen peroxide (H2O2), reduced glutathione (GSH), oxidized glutathione (GSSG), nitrites, superoxide dismutase activity (SOD), catalase activity (CAT) and glutathione-peroxidase activity (GPx). RESULTS: Levels of MDA, TAS, GSSG and H2O2 were significantly higher in athletes than in the control women. Football players had higher levels of O2- than the other two groups. Activity of SOD was higher in water polo players when compared with the football and control groups, CAT was increased in all athletes, while GPx did not differ among groups. CONCLUSIONS: Therefore, prolonged intensive training markedly increases oxidative stress in women, which depends on the type of sport. Lower concentration of O2- and increased activity of SOD in water polo players compared to football players suggest that mechanisms of adaptation of antioxidative defense are related to the type of exercise.",
publisher = "Edizioni Minerva Medica, Turin",
journal = "Journal of Sports Medicine and Physical Fitness",
title = "Redox balance in elite female athletes: Differences based on sport types",
volume = "56",
number = "1-2",
pages = "1-8",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1427"
}

Arsić, A., Vucić, V., Glibetić, M., Popović, T., Debeljak-Martačić, J., Cubrilo, D., Ahmetović, Z., Perić, D., Borozan, S., Đurić, D., Barudzić, N.,& Jakovljević, V.. (2016). Redox balance in elite female athletes: Differences based on sport types. in Journal of Sports Medicine and Physical Fitness
Edizioni Minerva Medica, Turin., 56(1-2), 1-8.
https://hdl.handle.net/21.15107/rcub_veterinar_1427

Arsić A, Vucić V, Glibetić M, Popović T, Debeljak-Martačić J, Cubrilo D, Ahmetović Z, Perić D, Borozan S, Đurić D, Barudzić N, Jakovljević V. Redox balance in elite female athletes: Differences based on sport types. in Journal of Sports Medicine and Physical Fitness. 2016;56(1-2):1-8.
https://hdl.handle.net/21.15107/rcub_veterinar_1427 .

Arsić, Aleksandra, Vucić, Vesna, Glibetić, Marija, Popović, Tamara, Debeljak-Martačić, Jasmina, Cubrilo, Dejan, Ahmetović, Zlatko, Perić, Dusan, Borozan, Sunčica, Đurić, Dragan, Barudzić, Nevena, Jakovljević, Vladimir, "Redox balance in elite female athletes: Differences based on sport types" in Journal of Sports Medicine and Physical Fitness, 56, no. 1-2 (2016):1-8,
https://hdl.handle.net/21.15107/rcub_veterinar_1427 .

TY  - JOUR
AU  - Debeljak-Martačić, Jasmina
AU  - Francuski, Jelena
AU  - Lužajić, Tijana
AU  - Vuković, Nemanja
AU  - Mojsilović, Slavko
AU  - Drndarević, Neda
AU  - Petakov, Marijana
AU  - Glibetić, Marija
AU  - Marković, Danica
AU  - Radovanović, Anita
AU  - Todorović, Vera
AU  - Kovačević-Filipović, Milica
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1145
AB  - Background/Aim. The last decade has been profoundly marked by persistent attempts to use ex vivo expanded and manipulated mesenchymal stem cells (MSCs), as a tool in different types of regenerative therapy. In the present study we described immunophenotype and the proliferative and differentiation potential of cells isolated from pulp remnants of exfoliated deciduous teeth in the final phase of root resorption. Methods. The initial adherent cell population from five donors was obtained by the outgrowth method. Colony forming unit-fibroblast (CFU-F) assay was performed in passage one. Cell expansion was performed until passage three and all tests were done until passage eight. Cells were labeled for early mesenchymal stem cells markers and analysis have been done using flow cytometry. The proliferative potential was assessed by cell counting in defined time points and population doubling time was calculated. Commercial media were used to induce osteoblastic, chondrogenic and adipogenic differentiation. Cytology and histology methods were used for analysis of differentiated cell morphology and extracellular matrix characteristics. Results. According to immunophenotype analyses all undifferentiated cells were positive for the mesenchymal stem cell markers: CD29 and CD73. Some cells expressed CD146 and CD106. The hematopoietic cell marker, CD34, was not detected. In passage one, incidence of CFU-F was 4.7 ± 0.5/100. Population doubling time did not change significantly during cell subcultivation and was in average 25 h. After induction of differentiation, the multicolony derived cell population had a tri-lineage differentiation potential, since mineralized matrix, cartilage-like tissue and adipocytes were successfully formed after three weeks of incubation. Conclusion. Altogether, these data suggest that remnants of deciduous teeth dental pulp contained cell populations with mesenchymal stem cell-like features, with a high proliferation and tri- lineage differentiation potential and that these cultures are suitable for further in vitro evaluation of cell based therapies.
AB  - Uvod/Cilj. Prošla dekada je bila posebno obeležena naporima na polju korišćenja ex vivo razvijenih i usmeravanih mezenhimskih matičnih ćelija (MSCs), kao sredstva za različite tipove regenerativne terapije. Cilj ove studije bio je da se utvrdi imunofenotip i potencijal za proliferaciju i diferencijaciju ćelija izolovanih iz zubne pulpe mlečnih zuba dece eksfoliranih u periodu kada je koren zuba bio u poslednjoj fazi resorpcije. Metode. Primarna adherentna populacija ćelija poreklom od pet donora dobijena je metodom eksplanta. Prisustvo progenitorskih ćelija koje obrazuju kolonije fibroblasta (CFU-F) pokazano je u prvoj pasaži. Do treće pasaže ćelije su ekspandirane, a potom korišćene za analiziranje. Imunofenotip je određen korišćenjem protočne citometrije. Proliferativni potencijal i vreme udvajanja ćelija (PDT) u kulturi je definisano na osnovu apsolutnog broja ćelija na početku i na kraju svake pasaže. Posle tronedeljne kultivacije ćelija u komercijalnim medijumima za stimulaciju osteogeneze, hondrogeneze i adipogeneze, citološkim i histološkim metodama je određena morfologija ćelija i karakteristike vanćelijskog matriksa. Rezultati. Antigeni koji karakterišu mezenhimske matične ćelije CD29 i CD73 su bili eksprimirani na svim nediferenciranim ćelijama, dok su antigeni CD146 i CD106 bili eksprimirani na ograničenom broju ćelija. Antigen CD34 (karakterističan za ćelije hematopoetske loze) nije bio eksprimiran. Incidencija CFU-F bila je 4,7 ± 0,5/100 ćelija. PDT se nije menjao tokom osam pasaža i u proseku je iznosio 25 h. Posle tronedeljne stimulacije diferencijacije u kulturama sa adipogenim medijumom došlo je do stvaranja ćelija sa masnim kapljicama, a u kulturama sa osteogenim medijumom došlo je do formiranja vanćelijskog matriksa sa deponovanim kalcijumovim solima. U kulturama sa hondrogenim medijumom došlo je do stvaranja tkiva sličnog hrskavici i vanćelijskog matriksa sa glikozaminoglikanima i kolagenom II. Zaključak. Zubna pulpa mlečnih zuba dece sadrži ćelijsku populaciju koja odgovara mezenhimskim matičnim ćelijama prema svojim karakteristikama, ima visok proliferativni potencijal i potencijal da se diferencira u tri ćelijske linije što je čini pogodnom za dalje in vitro analize i evaluaciju ćelijske terapije.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Characterization of deciduous teeth stem cells isolated from crown dental pulp
T1  - Karakterizacija matičnih ćelija izolovanih iz zubne pulpe mlečnih zuba dece
VL  - 71
IS  - 8
SP  - 735
EP  - 741
DO  - 10.2298/VSP1408735D
ER  - 

@article{
author = "Debeljak-Martačić, Jasmina and Francuski, Jelena and Lužajić, Tijana and Vuković, Nemanja and Mojsilović, Slavko and Drndarević, Neda and Petakov, Marijana and Glibetić, Marija and Marković, Danica and Radovanović, Anita and Todorović, Vera and Kovačević-Filipović, Milica",
year = "2014",
abstract = "Background/Aim. The last decade has been profoundly marked by persistent attempts to use ex vivo expanded and manipulated mesenchymal stem cells (MSCs), as a tool in different types of regenerative therapy. In the present study we described immunophenotype and the proliferative and differentiation potential of cells isolated from pulp remnants of exfoliated deciduous teeth in the final phase of root resorption. Methods. The initial adherent cell population from five donors was obtained by the outgrowth method. Colony forming unit-fibroblast (CFU-F) assay was performed in passage one. Cell expansion was performed until passage three and all tests were done until passage eight. Cells were labeled for early mesenchymal stem cells markers and analysis have been done using flow cytometry. The proliferative potential was assessed by cell counting in defined time points and population doubling time was calculated. Commercial media were used to induce osteoblastic, chondrogenic and adipogenic differentiation. Cytology and histology methods were used for analysis of differentiated cell morphology and extracellular matrix characteristics. Results. According to immunophenotype analyses all undifferentiated cells were positive for the mesenchymal stem cell markers: CD29 and CD73. Some cells expressed CD146 and CD106. The hematopoietic cell marker, CD34, was not detected. In passage one, incidence of CFU-F was 4.7 ± 0.5/100. Population doubling time did not change significantly during cell subcultivation and was in average 25 h. After induction of differentiation, the multicolony derived cell population had a tri-lineage differentiation potential, since mineralized matrix, cartilage-like tissue and adipocytes were successfully formed after three weeks of incubation. Conclusion. Altogether, these data suggest that remnants of deciduous teeth dental pulp contained cell populations with mesenchymal stem cell-like features, with a high proliferation and tri- lineage differentiation potential and that these cultures are suitable for further in vitro evaluation of cell based therapies., Uvod/Cilj. Prošla dekada je bila posebno obeležena naporima na polju korišćenja ex vivo razvijenih i usmeravanih mezenhimskih matičnih ćelija (MSCs), kao sredstva za različite tipove regenerativne terapije. Cilj ove studije bio je da se utvrdi imunofenotip i potencijal za proliferaciju i diferencijaciju ćelija izolovanih iz zubne pulpe mlečnih zuba dece eksfoliranih u periodu kada je koren zuba bio u poslednjoj fazi resorpcije. Metode. Primarna adherentna populacija ćelija poreklom od pet donora dobijena je metodom eksplanta. Prisustvo progenitorskih ćelija koje obrazuju kolonije fibroblasta (CFU-F) pokazano je u prvoj pasaži. Do treće pasaže ćelije su ekspandirane, a potom korišćene za analiziranje. Imunofenotip je određen korišćenjem protočne citometrije. Proliferativni potencijal i vreme udvajanja ćelija (PDT) u kulturi je definisano na osnovu apsolutnog broja ćelija na početku i na kraju svake pasaže. Posle tronedeljne kultivacije ćelija u komercijalnim medijumima za stimulaciju osteogeneze, hondrogeneze i adipogeneze, citološkim i histološkim metodama je određena morfologija ćelija i karakteristike vanćelijskog matriksa. Rezultati. Antigeni koji karakterišu mezenhimske matične ćelije CD29 i CD73 su bili eksprimirani na svim nediferenciranim ćelijama, dok su antigeni CD146 i CD106 bili eksprimirani na ograničenom broju ćelija. Antigen CD34 (karakterističan za ćelije hematopoetske loze) nije bio eksprimiran. Incidencija CFU-F bila je 4,7 ± 0,5/100 ćelija. PDT se nije menjao tokom osam pasaža i u proseku je iznosio 25 h. Posle tronedeljne stimulacije diferencijacije u kulturama sa adipogenim medijumom došlo je do stvaranja ćelija sa masnim kapljicama, a u kulturama sa osteogenim medijumom došlo je do formiranja vanćelijskog matriksa sa deponovanim kalcijumovim solima. U kulturama sa hondrogenim medijumom došlo je do stvaranja tkiva sličnog hrskavici i vanćelijskog matriksa sa glikozaminoglikanima i kolagenom II. Zaključak. Zubna pulpa mlečnih zuba dece sadrži ćelijsku populaciju koja odgovara mezenhimskim matičnim ćelijama prema svojim karakteristikama, ima visok proliferativni potencijal i potencijal da se diferencira u tri ćelijske linije što je čini pogodnom za dalje in vitro analize i evaluaciju ćelijske terapije.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Characterization of deciduous teeth stem cells isolated from crown dental pulp, Karakterizacija matičnih ćelija izolovanih iz zubne pulpe mlečnih zuba dece",
volume = "71",
number = "8",
pages = "735-741",
doi = "10.2298/VSP1408735D"
}

Debeljak-Martačić, J., Francuski, J., Lužajić, T., Vuković, N., Mojsilović, S., Drndarević, N., Petakov, M., Glibetić, M., Marković, D., Radovanović, A., Todorović, V.,& Kovačević-Filipović, M.. (2014). Characterization of deciduous teeth stem cells isolated from crown dental pulp. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 71(8), 735-741.
https://doi.org/10.2298/VSP1408735D

Debeljak-Martačić J, Francuski J, Lužajić T, Vuković N, Mojsilović S, Drndarević N, Petakov M, Glibetić M, Marković D, Radovanović A, Todorović V, Kovačević-Filipović M. Characterization of deciduous teeth stem cells isolated from crown dental pulp. in Vojnosanitetski pregled. 2014;71(8):735-741.
doi:10.2298/VSP1408735D .

Debeljak-Martačić, Jasmina, Francuski, Jelena, Lužajić, Tijana, Vuković, Nemanja, Mojsilović, Slavko, Drndarević, Neda, Petakov, Marijana, Glibetić, Marija, Marković, Danica, Radovanović, Anita, Todorović, Vera, Kovačević-Filipović, Milica, "Characterization of deciduous teeth stem cells isolated from crown dental pulp" in Vojnosanitetski pregled, 71, no. 8 (2014):735-741,
https://doi.org/10.2298/VSP1408735D . .

TY  - CONF
AU  - Popović, Tamara
AU  - Borozan, Sunčica
AU  - Arsić, Aleksandra
AU  - Debeljak-Martačić, Jasmina
AU  - de Luka, Silvio
AU  - Glibetić, Marija
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1036
PB  - Elsevier Science Inc, New York
C3  - Free Radical Biology and Medicine
T1  - Correlation between oxidative stress and fatty acids profiles in plasma phospholipids after fish oil treatment in aged male rats
VL  - 65
SP  - S43
EP  - S43
DO  - 10.1016/j.freeradbiomed.2013.08.065
ER  - 

@conference{
author = "Popović, Tamara and Borozan, Sunčica and Arsić, Aleksandra and Debeljak-Martačić, Jasmina and de Luka, Silvio and Glibetić, Marija",
year = "2013",
publisher = "Elsevier Science Inc, New York",
journal = "Free Radical Biology and Medicine",
title = "Correlation between oxidative stress and fatty acids profiles in plasma phospholipids after fish oil treatment in aged male rats",
volume = "65",
pages = "S43-S43",
doi = "10.1016/j.freeradbiomed.2013.08.065"
}

Popović, T., Borozan, S., Arsić, A., Debeljak-Martačić, J., de Luka, S.,& Glibetić, M.. (2013). Correlation between oxidative stress and fatty acids profiles in plasma phospholipids after fish oil treatment in aged male rats. in Free Radical Biology and Medicine
Elsevier Science Inc, New York., 65, S43-S43.
https://doi.org/10.1016/j.freeradbiomed.2013.08.065

Popović T, Borozan S, Arsić A, Debeljak-Martačić J, de Luka S, Glibetić M. Correlation between oxidative stress and fatty acids profiles in plasma phospholipids after fish oil treatment in aged male rats. in Free Radical Biology and Medicine. 2013;65:S43-S43.
doi:10.1016/j.freeradbiomed.2013.08.065 .

Popović, Tamara, Borozan, Sunčica, Arsić, Aleksandra, Debeljak-Martačić, Jasmina, de Luka, Silvio, Glibetić, Marija, "Correlation between oxidative stress and fatty acids profiles in plasma phospholipids after fish oil treatment in aged male rats" in Free Radical Biology and Medicine, 65 (2013):S43-S43,
https://doi.org/10.1016/j.freeradbiomed.2013.08.065 . .

TY  - JOUR
AU  - Rasic-Milutinović, Zorica
AU  - Popović, Tamara
AU  - Peruničić-Peković, Gordana
AU  - Arsić, Aleksandra
AU  - Borozan, Sunčica
AU  - Glibetić, Marija
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/878
AB  - Background. Serum paraoxonase-1(PON-1) activity is decreased in clinical conditions associated with low high-density lipoprotein cholesterol (HDL-C), increased lipid peroxidation and low-grade chronic inflammation, as in type 2 diabetes mellitus (T2DM). Until now there are no data about the association of any fatty acid (FA) with PON-1 activity in T2DM. Methods. Twenty patients with T2DM and 16 healthy controls were included in this cross-sectional study. Serum PON-1 activity, superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activity as well as plasma glucose, HbA1c, lipids, high-sensitivity C-reactive protein (hs-CRP) and insulin resistance, homeostasis model assessment (HOMA-IR) were measured. The preparation of FA methyl esters and their gas chromatography (GC) analysis were also performed. Results. HbA1c, plasma insulin, HOMA-IR and triglycerides were higher in patients with T2DM, whereas HDL-C was lower in those subjects. Levels of pro-oxidative enzyme malondialdehyde (MDA) and hs-CRP were significantly higher, and antioxidative enzymes SOD and PON-1 activity were decreased in T2DM patients. N-6 PUFAs were higher in T2DM patients, particularly linoleic acid (LA, 18:2 n-6) and arachidonic acid (AA, 20:4 n-6), whereas n-3 PUFA, docosahexaenoic acid (DHA, 22:6 n-3) was lower in T2DM patients. Using regression analysis, we have shown that only LA and DHA independently predicted PON-1 activity of all participants, particularly in patients with T2DM. Conclusions. Decreased serum PON-1 activity may, in part, be influenced by higher levels of LA and lower levels of DHA in patients with 12DM. Prospective, randomized studies are necessary to confirm these preliminary findings.
PB  - Elsevier Science Inc, New York
T2  - Archives of Medical Research
T1  - Lower Serum Paraoxonase-1 Activity Is Related to Linoleic and Docosahexanoic Fatty Acids in Type 2 Diabetic Patients
VL  - 43
IS  - 1
SP  - 75
EP  - 82
DO  - 10.1016/j.arcmed.2011.12.008
ER  - 

@article{
author = "Rasic-Milutinović, Zorica and Popović, Tamara and Peruničić-Peković, Gordana and Arsić, Aleksandra and Borozan, Sunčica and Glibetić, Marija",
year = "2012",
abstract = "Background. Serum paraoxonase-1(PON-1) activity is decreased in clinical conditions associated with low high-density lipoprotein cholesterol (HDL-C), increased lipid peroxidation and low-grade chronic inflammation, as in type 2 diabetes mellitus (T2DM). Until now there are no data about the association of any fatty acid (FA) with PON-1 activity in T2DM. Methods. Twenty patients with T2DM and 16 healthy controls were included in this cross-sectional study. Serum PON-1 activity, superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activity as well as plasma glucose, HbA1c, lipids, high-sensitivity C-reactive protein (hs-CRP) and insulin resistance, homeostasis model assessment (HOMA-IR) were measured. The preparation of FA methyl esters and their gas chromatography (GC) analysis were also performed. Results. HbA1c, plasma insulin, HOMA-IR and triglycerides were higher in patients with T2DM, whereas HDL-C was lower in those subjects. Levels of pro-oxidative enzyme malondialdehyde (MDA) and hs-CRP were significantly higher, and antioxidative enzymes SOD and PON-1 activity were decreased in T2DM patients. N-6 PUFAs were higher in T2DM patients, particularly linoleic acid (LA, 18:2 n-6) and arachidonic acid (AA, 20:4 n-6), whereas n-3 PUFA, docosahexaenoic acid (DHA, 22:6 n-3) was lower in T2DM patients. Using regression analysis, we have shown that only LA and DHA independently predicted PON-1 activity of all participants, particularly in patients with T2DM. Conclusions. Decreased serum PON-1 activity may, in part, be influenced by higher levels of LA and lower levels of DHA in patients with 12DM. Prospective, randomized studies are necessary to confirm these preliminary findings.",
publisher = "Elsevier Science Inc, New York",
journal = "Archives of Medical Research",
title = "Lower Serum Paraoxonase-1 Activity Is Related to Linoleic and Docosahexanoic Fatty Acids in Type 2 Diabetic Patients",
volume = "43",
number = "1",
pages = "75-82",
doi = "10.1016/j.arcmed.2011.12.008"
}

Rasic-Milutinović, Z., Popović, T., Peruničić-Peković, G., Arsić, A., Borozan, S.,& Glibetić, M.. (2012). Lower Serum Paraoxonase-1 Activity Is Related to Linoleic and Docosahexanoic Fatty Acids in Type 2 Diabetic Patients. in Archives of Medical Research
Elsevier Science Inc, New York., 43(1), 75-82.
https://doi.org/10.1016/j.arcmed.2011.12.008

Rasic-Milutinović Z, Popović T, Peruničić-Peković G, Arsić A, Borozan S, Glibetić M. Lower Serum Paraoxonase-1 Activity Is Related to Linoleic and Docosahexanoic Fatty Acids in Type 2 Diabetic Patients. in Archives of Medical Research. 2012;43(1):75-82.
doi:10.1016/j.arcmed.2011.12.008 .

Rasic-Milutinović, Zorica, Popović, Tamara, Peruničić-Peković, Gordana, Arsić, Aleksandra, Borozan, Sunčica, Glibetić, Marija, "Lower Serum Paraoxonase-1 Activity Is Related to Linoleic and Docosahexanoic Fatty Acids in Type 2 Diabetic Patients" in Archives of Medical Research, 43, no. 1 (2012):75-82,
https://doi.org/10.1016/j.arcmed.2011.12.008 . .