TY  - JOUR
AU  - Kovačević-Filipović, Milica
AU  - Okić, Ivana
AU  - Petrićević, Tanja
AU  - Mojsilović, Slavko
AU  - Krstić, Aleksandra
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
AU  - Milenković, P.
AU  - Petakov, Marijana
AU  - Radovanović, Anita
AU  - Božić, Tatjana
AU  - Ivanović, Z.
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/661
AB  - Hamster, a hibernating animal, is an important experimental model in research on the influence of hypothermia on different physiological processes. A simple procedure for cultivation and identification of hamster hematopoetic stem cells (HSC) and hematopoetic progenitor cells (HPC) is a premise for a successful investigation upon hypothermia effects on hematopoiesis. The aim of this work was to evaluate the utilization of commercially available methylcellulose media (MC) and recombinant mouse and human cytokines for hamster HSC and HPC assays, in order to enable further studies on these cells. Hamster bone marrow mononuclear cells (BMMNC) were plated in MC containing cytokines that support mouse or human HPC growth. Also, BMMNC were resuspended in cytokine supplemented liquid media and incubated for 5 weeks with a four day monitoring of viable cell number. We demonstrated that hamster hematopoietic progenitor cells committed for erythroid lineage and myeloid lineage successfully formed recognizable colonies in both mouse and human MC, while multipotent progenitor cells formed colonies only in mouse MC. We also defined conditions for the evaluation of hamster HSC activity in liquid cultures, based on continuous 5 weeks HSC proliferation. The obtained results verify the utilization of mouse specific MC for further research on hamster HPC biology during hypothermia.
AB  - Fiziološka hibernacija u koju hrčci ulaze prilikom izlaganja niskim temperaturama, čini ove životinje zanimljivim eksperimentalnim modelom za ispitivanje hematopoeze u uslovima hipotermije. Preduslov za ovo ispitivanje je postojanje jednostavne metode za kultivaciju i identifikaciju hematopoetskih ćelija hrčka. Cilj ovog rada je bio da se ispita mogućnost kultivacije progenitorskih ćelija hematopoeze hrčka u kompletnoj metil celulozi dizajniranoj za kultivaciju mišijih i humanih hematopoetskih ćelija, kao i da se odrede optimalni uslovi za kultivaciju matičnih ćelija hematopoeze hrčka u tečnoj kulturi. Mononuklearne ćelije kostne srži hrčka su posađene u metil celulozu i u tečnu kulturu. Oba medijuma su sadržala kombinacije rekombinantnih mišijih i/ili humanih citokina. Kolonije progenitorskih ćelija opredeljenih za mijelopoezu i opredeljenih za eritropoezu su se formirale u metil celulozi dizajniranoj za kultivaciju mišijih i humanih hematopoetskih ćelija, dok su se primitivnije kolonije sastavljene od oba tipa ćelija (mijeloidna i eritrocitna loza) formirale samo u metil celulozi dizajniranoj za kultivaciju mišijih hematopoetskih ćelija. Osim toga, populacija matičnih ćelija hematopoeze hrčka je proliferisala u tečnim kulturama tokom 5 nedelja bez znakova opadanja proliferativnog potencijala. Ova istraživanja pokazuju da se primenjene metode mogu uspešno koristiti za ispitivanje hematopoeze kod hrčka.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Cultivation of hamster bone marrow haematopoietic stem and progenitor cells
T1  - Kultivacija matičnih i progenitorskih ćelija hematopoeze iz kostne srži hrčka
VL  - 60
IS  - 1
SP  - 3
EP  - 14
DO  - 10.2298/AVB1001003K
ER  - 

@article{
author = "Kovačević-Filipović, Milica and Okić, Ivana and Petrićević, Tanja and Mojsilović, Slavko and Krstić, Aleksandra and Jovčić, Gordana and Bugarski, Diana and Milenković, P. and Petakov, Marijana and Radovanović, Anita and Božić, Tatjana and Ivanović, Z.",
year = "2010",
abstract = "Hamster, a hibernating animal, is an important experimental model in research on the influence of hypothermia on different physiological processes. A simple procedure for cultivation and identification of hamster hematopoetic stem cells (HSC) and hematopoetic progenitor cells (HPC) is a premise for a successful investigation upon hypothermia effects on hematopoiesis. The aim of this work was to evaluate the utilization of commercially available methylcellulose media (MC) and recombinant mouse and human cytokines for hamster HSC and HPC assays, in order to enable further studies on these cells. Hamster bone marrow mononuclear cells (BMMNC) were plated in MC containing cytokines that support mouse or human HPC growth. Also, BMMNC were resuspended in cytokine supplemented liquid media and incubated for 5 weeks with a four day monitoring of viable cell number. We demonstrated that hamster hematopoietic progenitor cells committed for erythroid lineage and myeloid lineage successfully formed recognizable colonies in both mouse and human MC, while multipotent progenitor cells formed colonies only in mouse MC. We also defined conditions for the evaluation of hamster HSC activity in liquid cultures, based on continuous 5 weeks HSC proliferation. The obtained results verify the utilization of mouse specific MC for further research on hamster HPC biology during hypothermia., Fiziološka hibernacija u koju hrčci ulaze prilikom izlaganja niskim temperaturama, čini ove životinje zanimljivim eksperimentalnim modelom za ispitivanje hematopoeze u uslovima hipotermije. Preduslov za ovo ispitivanje je postojanje jednostavne metode za kultivaciju i identifikaciju hematopoetskih ćelija hrčka. Cilj ovog rada je bio da se ispita mogućnost kultivacije progenitorskih ćelija hematopoeze hrčka u kompletnoj metil celulozi dizajniranoj za kultivaciju mišijih i humanih hematopoetskih ćelija, kao i da se odrede optimalni uslovi za kultivaciju matičnih ćelija hematopoeze hrčka u tečnoj kulturi. Mononuklearne ćelije kostne srži hrčka su posađene u metil celulozu i u tečnu kulturu. Oba medijuma su sadržala kombinacije rekombinantnih mišijih i/ili humanih citokina. Kolonije progenitorskih ćelija opredeljenih za mijelopoezu i opredeljenih za eritropoezu su se formirale u metil celulozi dizajniranoj za kultivaciju mišijih i humanih hematopoetskih ćelija, dok su se primitivnije kolonije sastavljene od oba tipa ćelija (mijeloidna i eritrocitna loza) formirale samo u metil celulozi dizajniranoj za kultivaciju mišijih hematopoetskih ćelija. Osim toga, populacija matičnih ćelija hematopoeze hrčka je proliferisala u tečnim kulturama tokom 5 nedelja bez znakova opadanja proliferativnog potencijala. Ova istraživanja pokazuju da se primenjene metode mogu uspešno koristiti za ispitivanje hematopoeze kod hrčka.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Cultivation of hamster bone marrow haematopoietic stem and progenitor cells, Kultivacija matičnih i progenitorskih ćelija hematopoeze iz kostne srži hrčka",
volume = "60",
number = "1",
pages = "3-14",
doi = "10.2298/AVB1001003K"
}

Kovačević-Filipović, M., Okić, I., Petrićević, T., Mojsilović, S., Krstić, A., Jovčić, G., Bugarski, D., Milenković, P., Petakov, M., Radovanović, A., Božić, T.,& Ivanović, Z.. (2010). Cultivation of hamster bone marrow haematopoietic stem and progenitor cells. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(1), 3-14.
https://doi.org/10.2298/AVB1001003K

Kovačević-Filipović M, Okić I, Petrićević T, Mojsilović S, Krstić A, Jovčić G, Bugarski D, Milenković P, Petakov M, Radovanović A, Božić T, Ivanović Z. Cultivation of hamster bone marrow haematopoietic stem and progenitor cells. in Acta Veterinaria-Beograd. 2010;60(1):3-14.
doi:10.2298/AVB1001003K .

Kovačević-Filipović, Milica, Okić, Ivana, Petrićević, Tanja, Mojsilović, Slavko, Krstić, Aleksandra, Jovčić, Gordana, Bugarski, Diana, Milenković, P., Petakov, Marijana, Radovanović, Anita, Božić, Tatjana, Ivanović, Z., "Cultivation of hamster bone marrow haematopoietic stem and progenitor cells" in Acta Veterinaria-Beograd, 60, no. 1 (2010):3-14,
https://doi.org/10.2298/AVB1001003K . .

TY  - JOUR
AU  - Ivanović, Zoran
AU  - Kovačević-Filipović, Milica
AU  - Jeanne, Michel
AU  - Ardilouze, Leslie
AU  - Bertot, Anne
AU  - Szyporta, Milene
AU  - Hermitte, Francis
AU  - Lafarge, Xavier
AU  - Duchez, Pascale
AU  - Vlaški, Marija
AU  - Milpied, Noel
AU  - Pavlović, Mirjana
AU  - Praloran, Vincent
AU  - Boiron, Jean-Michel
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/753
AB  - BACKGROUND: The classification of patients into good or poor mobilizers is based on CD34+ cell count in their peripheral blood (PB) after granulocyte-colony-stimulating factor (G-CSF) injection. We hypothesized that, apart from their mobilization from marrow to the blood, the response to G-CSF of CD34+ cells also includes activation of proliferation, metabolic activity, and proliferative capacity. STUDY DESIGN AND METHODS: Mobilized PB CD34+ cells purified from samples obtained by cytapheresis of multiple myeloma or non-Hodgkins lymphoma patients of both good (> 50 CD34+ cells/mu L) and poor (< 50 CD34+ cells/mu L) mobilizers were studied. The initial cell cycle state of CD34+ cells after selection and their kinetics of activation (exit from G(0) phase) during ex vivo culture were analyzed. Their proliferative capacity was estimated on the basis of ex vivo generation of total cells, CD34+ cells, and colony-forming cells (CFCs), in a standardized expansion culture. Indirect insight in metabolic activity was obtained on the basis of their survival (viability and apoptosis follow-up) during the 7-day-long conservation in hypothermia (4 degrees C) in the air or in atmosphere containing 3% O-2/6% CO2. RESULTS: CD34+ cells obtained from good mobilizers were in lower proportion in the G(0) phase, their activation in a cytokine-stimulated culture was accelerated, and they exhibited a lower ex vivo expansion efficiency than those from poor mobilizers. The resistance to hypothermia of good immobilizers CD34+ cells is impaired. CONCLUSION: A good response to G-CSF mobilization treatment is associated with a higher degree of proliferative and metabolic activation of mobilized CD34+ cells with a decrease in their expansion capacity.
PB  - Wiley, Hoboken
T2  - Transfusion
T1  - CD34+cells obtained from ""good mobilizers"" are more activated and exhibit lower ex vivo expansion efficiency than their counterparts from ""poor mobilizers""
VL  - 50
IS  - 1
SP  - 120
EP  - 127
DO  - 10.1111/j.1537-2995.2009.02436.x
ER  - 

@article{
author = "Ivanović, Zoran and Kovačević-Filipović, Milica and Jeanne, Michel and Ardilouze, Leslie and Bertot, Anne and Szyporta, Milene and Hermitte, Francis and Lafarge, Xavier and Duchez, Pascale and Vlaški, Marija and Milpied, Noel and Pavlović, Mirjana and Praloran, Vincent and Boiron, Jean-Michel",
year = "2010",
abstract = "BACKGROUND: The classification of patients into good or poor mobilizers is based on CD34+ cell count in their peripheral blood (PB) after granulocyte-colony-stimulating factor (G-CSF) injection. We hypothesized that, apart from their mobilization from marrow to the blood, the response to G-CSF of CD34+ cells also includes activation of proliferation, metabolic activity, and proliferative capacity. STUDY DESIGN AND METHODS: Mobilized PB CD34+ cells purified from samples obtained by cytapheresis of multiple myeloma or non-Hodgkins lymphoma patients of both good (> 50 CD34+ cells/mu L) and poor (< 50 CD34+ cells/mu L) mobilizers were studied. The initial cell cycle state of CD34+ cells after selection and their kinetics of activation (exit from G(0) phase) during ex vivo culture were analyzed. Their proliferative capacity was estimated on the basis of ex vivo generation of total cells, CD34+ cells, and colony-forming cells (CFCs), in a standardized expansion culture. Indirect insight in metabolic activity was obtained on the basis of their survival (viability and apoptosis follow-up) during the 7-day-long conservation in hypothermia (4 degrees C) in the air or in atmosphere containing 3% O-2/6% CO2. RESULTS: CD34+ cells obtained from good mobilizers were in lower proportion in the G(0) phase, their activation in a cytokine-stimulated culture was accelerated, and they exhibited a lower ex vivo expansion efficiency than those from poor mobilizers. The resistance to hypothermia of good immobilizers CD34+ cells is impaired. CONCLUSION: A good response to G-CSF mobilization treatment is associated with a higher degree of proliferative and metabolic activation of mobilized CD34+ cells with a decrease in their expansion capacity.",
publisher = "Wiley, Hoboken",
journal = "Transfusion",
title = "CD34+cells obtained from ""good mobilizers"" are more activated and exhibit lower ex vivo expansion efficiency than their counterparts from ""poor mobilizers""",
volume = "50",
number = "1",
pages = "120-127",
doi = "10.1111/j.1537-2995.2009.02436.x"
}

Ivanović, Z., Kovačević-Filipović, M., Jeanne, M., Ardilouze, L., Bertot, A., Szyporta, M., Hermitte, F., Lafarge, X., Duchez, P., Vlaški, M., Milpied, N., Pavlović, M., Praloran, V.,& Boiron, J.. (2010). CD34+cells obtained from ""good mobilizers"" are more activated and exhibit lower ex vivo expansion efficiency than their counterparts from ""poor mobilizers"". in Transfusion
Wiley, Hoboken., 50(1), 120-127.
https://doi.org/10.1111/j.1537-2995.2009.02436.x

Ivanović Z, Kovačević-Filipović M, Jeanne M, Ardilouze L, Bertot A, Szyporta M, Hermitte F, Lafarge X, Duchez P, Vlaški M, Milpied N, Pavlović M, Praloran V, Boiron J. CD34+cells obtained from ""good mobilizers"" are more activated and exhibit lower ex vivo expansion efficiency than their counterparts from ""poor mobilizers"". in Transfusion. 2010;50(1):120-127.
doi:10.1111/j.1537-2995.2009.02436.x .

Ivanović, Zoran, Kovačević-Filipović, Milica, Jeanne, Michel, Ardilouze, Leslie, Bertot, Anne, Szyporta, Milene, Hermitte, Francis, Lafarge, Xavier, Duchez, Pascale, Vlaški, Marija, Milpied, Noel, Pavlović, Mirjana, Praloran, Vincent, Boiron, Jean-Michel, "CD34+cells obtained from ""good mobilizers"" are more activated and exhibit lower ex vivo expansion efficiency than their counterparts from ""poor mobilizers""" in Transfusion, 50, no. 1 (2010):120-127,
https://doi.org/10.1111/j.1537-2995.2009.02436.x . .

TY  - JOUR
AU  - Jeanne, Michel
AU  - Kovačević-Filipović, Milica
AU  - Szyporta, Milene
AU  - Vlaški, Marija
AU  - Hermitte, Francis
AU  - Lafarge, Xavier
AU  - Duchez, Pascale
AU  - Boiron, Jean-Michel
AU  - Praloran, Vincent
AU  - Ivanović, Zoran
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/585
AB  - BACKGROUND: During short-term storage of hematopoietic cells (HCs) at 4 degrees C a substantial decline in number and in functional capacity of progenitors occurs after 3 days. We hypothesized that physiologic O-2 and CO2 concentrations of hematopoietic tissue microenvironment (approx. 3% O-2 and approx. 6% CO2) could improve cell viability and functionality during storage at 4 degrees C. STUDY DESIGN AND METHODS: Mobilized peripheral blood (PB) CD34+ cells from multiple myeloma or non-Hodgkins lymphoma patients were stored in flasks containing air (approx. 20% O-2 and approx. 0.05% CO2) or 3% O-2/6% CO2 atmosphere, for 3, 5, and 7 days at 4 degrees C. The total number of cells, the number of cells in G0 or G1 phase of cell cycle, and the apoptosis rate were determined. The functional capacity of stored cells was assessed by the capacity of progenitors to form colonies in methylcellulose (colony-forming cells [CFCs]) and of stem cells to repopulate the bone marrow (BM) of immunodeficient mice (SCID-repopulating cell [SRC] assay). RESULTS: The total number of viable cells and cells in G1 phase as well as the number of total CFCs were significantly higher at 3% O-2/6% CO2 than in air at all time points. Cells in G0 phase and SRC were equally preserved in both conditions. CONCLUSION: Atmosphere with low O-2 and high CO2 concentration (3% O-2/6% CO2) in hypothermia (+4 degrees C) during 7 days of storage prevents cell damage and preserves a high number of functional HSCs and progenitors mobilized in PB by granulocyte-colony-stimulating factor.
PB  - Wiley, Hoboken
T2  - Transfusion
T1  - Low-oxygen and high-carbon-dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia
VL  - 49
IS  - 8
SP  - 1738
EP  - 1746
DO  - 10.1111/j.1537-2995.2009.02191.x
ER  - 

@article{
author = "Jeanne, Michel and Kovačević-Filipović, Milica and Szyporta, Milene and Vlaški, Marija and Hermitte, Francis and Lafarge, Xavier and Duchez, Pascale and Boiron, Jean-Michel and Praloran, Vincent and Ivanović, Zoran",
year = "2009",
abstract = "BACKGROUND: During short-term storage of hematopoietic cells (HCs) at 4 degrees C a substantial decline in number and in functional capacity of progenitors occurs after 3 days. We hypothesized that physiologic O-2 and CO2 concentrations of hematopoietic tissue microenvironment (approx. 3% O-2 and approx. 6% CO2) could improve cell viability and functionality during storage at 4 degrees C. STUDY DESIGN AND METHODS: Mobilized peripheral blood (PB) CD34+ cells from multiple myeloma or non-Hodgkins lymphoma patients were stored in flasks containing air (approx. 20% O-2 and approx. 0.05% CO2) or 3% O-2/6% CO2 atmosphere, for 3, 5, and 7 days at 4 degrees C. The total number of cells, the number of cells in G0 or G1 phase of cell cycle, and the apoptosis rate were determined. The functional capacity of stored cells was assessed by the capacity of progenitors to form colonies in methylcellulose (colony-forming cells [CFCs]) and of stem cells to repopulate the bone marrow (BM) of immunodeficient mice (SCID-repopulating cell [SRC] assay). RESULTS: The total number of viable cells and cells in G1 phase as well as the number of total CFCs were significantly higher at 3% O-2/6% CO2 than in air at all time points. Cells in G0 phase and SRC were equally preserved in both conditions. CONCLUSION: Atmosphere with low O-2 and high CO2 concentration (3% O-2/6% CO2) in hypothermia (+4 degrees C) during 7 days of storage prevents cell damage and preserves a high number of functional HSCs and progenitors mobilized in PB by granulocyte-colony-stimulating factor.",
publisher = "Wiley, Hoboken",
journal = "Transfusion",
title = "Low-oxygen and high-carbon-dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia",
volume = "49",
number = "8",
pages = "1738-1746",
doi = "10.1111/j.1537-2995.2009.02191.x"
}

Jeanne, M., Kovačević-Filipović, M., Szyporta, M., Vlaški, M., Hermitte, F., Lafarge, X., Duchez, P., Boiron, J., Praloran, V.,& Ivanović, Z.. (2009). Low-oxygen and high-carbon-dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia. in Transfusion
Wiley, Hoboken., 49(8), 1738-1746.
https://doi.org/10.1111/j.1537-2995.2009.02191.x

Jeanne M, Kovačević-Filipović M, Szyporta M, Vlaški M, Hermitte F, Lafarge X, Duchez P, Boiron J, Praloran V, Ivanović Z. Low-oxygen and high-carbon-dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia. in Transfusion. 2009;49(8):1738-1746.
doi:10.1111/j.1537-2995.2009.02191.x .

Jeanne, Michel, Kovačević-Filipović, Milica, Szyporta, Milene, Vlaški, Marija, Hermitte, Francis, Lafarge, Xavier, Duchez, Pascale, Boiron, Jean-Michel, Praloran, Vincent, Ivanović, Zoran, "Low-oxygen and high-carbon-dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia" in Transfusion, 49, no. 8 (2009):1738-1746,
https://doi.org/10.1111/j.1537-2995.2009.02191.x . .

TY  - JOUR
AU  - Kovačević-Filipović, Milica
AU  - Petakov, Marijana
AU  - Hermitte, Francis
AU  - Debeissat, Christelle
AU  - Krstić, Aleksandra
AU  - Jovčić, Gordana
AU  - Biligarski, Dijana
AU  - Lafarge, Xavier
AU  - Milenković, Pavle
AU  - Praloran, Vincent
AU  - Ivanović, Zoran
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/458
AB  - Low O-2 concentration (1%) favors the self-renewal of hematopoietic stem cells and inhibits committed progenitors (CFC). Since IL-6 influences both stem cells and committed progenitors at 20% O-2, we studied its effects in cultures at 1% O-2. The pre-CFC activity in Lin- population of mouse bone marrow was analyzed following 10 days of serum-free culture in medium (LCI) supplemented with IL-3 with and without IL-6, at 20 and 1% O-2 and phenotypic differentiation and proliferative history monitored. The IL-6 receptor expression and initiation of VEGF-A synthesis were also investigated. At 20% O-2, the effects of IL-6 on pre-CFC were negligible but effects on CFC were apparent; conversely, at 1% O-2, the IL-6 enhances activity of pre-CFC but not of CFC. Unlike at 20% O-2, at 1% O-2 a subpopulation of cells remained Lin- in spite of extensive proliferation. However, the absolute number of Lin- cells, did not correlate with pre-CFC activity. A relative increase in VEGF transcripts at 1% O-2 in presence of IL-3 alone was enhanced by the addition of IL-6. IL-6 enhanced pre-CFC activity at 1% O-2 and this was correlated to the induction of VEGF. These data reinforce the concept that physiologically low oxygenation of bone marrow is a regulator of stem cell maintenance. Since the 20% O-2 does not exist in tissues in vivo, further studies in vitro at lower O-2 concentrations should revise our knowledge relating to cytokine effects on stem and progenitor cells.
PB  - Wiley-Liss, Hoboken
T2  - Journal of Cellular Physiology
T1  - Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)
VL  - 212
IS  - 1
SP  - 68
EP  - 75
DO  - 10.1002/jcp.21003
ER  - 

@article{
author = "Kovačević-Filipović, Milica and Petakov, Marijana and Hermitte, Francis and Debeissat, Christelle and Krstić, Aleksandra and Jovčić, Gordana and Biligarski, Dijana and Lafarge, Xavier and Milenković, Pavle and Praloran, Vincent and Ivanović, Zoran",
year = "2007",
abstract = "Low O-2 concentration (1%) favors the self-renewal of hematopoietic stem cells and inhibits committed progenitors (CFC). Since IL-6 influences both stem cells and committed progenitors at 20% O-2, we studied its effects in cultures at 1% O-2. The pre-CFC activity in Lin- population of mouse bone marrow was analyzed following 10 days of serum-free culture in medium (LCI) supplemented with IL-3 with and without IL-6, at 20 and 1% O-2 and phenotypic differentiation and proliferative history monitored. The IL-6 receptor expression and initiation of VEGF-A synthesis were also investigated. At 20% O-2, the effects of IL-6 on pre-CFC were negligible but effects on CFC were apparent; conversely, at 1% O-2, the IL-6 enhances activity of pre-CFC but not of CFC. Unlike at 20% O-2, at 1% O-2 a subpopulation of cells remained Lin- in spite of extensive proliferation. However, the absolute number of Lin- cells, did not correlate with pre-CFC activity. A relative increase in VEGF transcripts at 1% O-2 in presence of IL-3 alone was enhanced by the addition of IL-6. IL-6 enhanced pre-CFC activity at 1% O-2 and this was correlated to the induction of VEGF. These data reinforce the concept that physiologically low oxygenation of bone marrow is a regulator of stem cell maintenance. Since the 20% O-2 does not exist in tissues in vivo, further studies in vitro at lower O-2 concentrations should revise our knowledge relating to cytokine effects on stem and progenitor cells.",
publisher = "Wiley-Liss, Hoboken",
journal = "Journal of Cellular Physiology",
title = "Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)",
volume = "212",
number = "1",
pages = "68-75",
doi = "10.1002/jcp.21003"
}

Kovačević-Filipović, M., Petakov, M., Hermitte, F., Debeissat, C., Krstić, A., Jovčić, G., Biligarski, D., Lafarge, X., Milenković, P., Praloran, V.,& Ivanović, Z.. (2007). Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC). in Journal of Cellular Physiology
Wiley-Liss, Hoboken., 212(1), 68-75.
https://doi.org/10.1002/jcp.21003

Kovačević-Filipović M, Petakov M, Hermitte F, Debeissat C, Krstić A, Jovčić G, Biligarski D, Lafarge X, Milenković P, Praloran V, Ivanović Z. Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC). in Journal of Cellular Physiology. 2007;212(1):68-75.
doi:10.1002/jcp.21003 .

Kovačević-Filipović, Milica, Petakov, Marijana, Hermitte, Francis, Debeissat, Christelle, Krstić, Aleksandra, Jovčić, Gordana, Biligarski, Dijana, Lafarge, Xavier, Milenković, Pavle, Praloran, Vincent, Ivanović, Zoran, "Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)" in Journal of Cellular Physiology, 212, no. 1 (2007):68-75,
https://doi.org/10.1002/jcp.21003 . .