TY  - JOUR
AU  - Zuber, Ivana
AU  - Lakićević, Brankica
AU  - Pietzka, Ariane
AU  - Milanov, Dubravka
AU  - Đorđević, Vesna
AU  - Karabasil, Nedjeljko
AU  - Teodorović, Vlado
AU  - Ruppitsch, Werner
AU  - Dimitrijević, Mirjana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1844
AB  - The presence of Listeria monocytogenes was evaluated in a small-scale meat processing facility in Montenegro during 2011-2014. L. monocytogenes isolates from traditional meat products and environmental swabs were subjected to a) molecular characterization b) serotyping by both multiplex PCR and next generation sequencing (NGS) c) potential antimicrobial resistance (AMR) was assessed by extraction of specific genes from NGS data and d) screening for the presence of some disinfectant resistance markers. Overall, traditional meat products were contaminated, most likely from incoming raw materials, with 4 major specific STs of L. monocytogenes (ST515, ST8, ST21, ST121) representing 4 clonal complexes (CC1, CC8, CC21, CC121) identified during the four-year period. These strains belonged to serogroup IIa which predominated, followed by IVb (ST515, CC1). The strains from environmental swabs belonged, exclusively, to ST21 and were isolated from cutting board and floor swabs in 2011. Furthermore, we found Tn6188, a novel transposon conferring tolerance to BC, to be specific to sequence type ST121. In addition, antimicrobial resistance genes mprF and fosX were present in clonal complexes CC21 and CC121, while complexes CC8 and CC1 exclusively harbored the mprF antimicrobial resistance gene.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Food Microbiology
T1  - Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014
VL  - 79
SP  - 116
EP  - 122
DO  - 10.1016/j.fm.2018.12.005
ER  - 

@article{
author = "Zuber, Ivana and Lakićević, Brankica and Pietzka, Ariane and Milanov, Dubravka and Đorđević, Vesna and Karabasil, Nedjeljko and Teodorović, Vlado and Ruppitsch, Werner and Dimitrijević, Mirjana",
year = "2019",
abstract = "The presence of Listeria monocytogenes was evaluated in a small-scale meat processing facility in Montenegro during 2011-2014. L. monocytogenes isolates from traditional meat products and environmental swabs were subjected to a) molecular characterization b) serotyping by both multiplex PCR and next generation sequencing (NGS) c) potential antimicrobial resistance (AMR) was assessed by extraction of specific genes from NGS data and d) screening for the presence of some disinfectant resistance markers. Overall, traditional meat products were contaminated, most likely from incoming raw materials, with 4 major specific STs of L. monocytogenes (ST515, ST8, ST21, ST121) representing 4 clonal complexes (CC1, CC8, CC21, CC121) identified during the four-year period. These strains belonged to serogroup IIa which predominated, followed by IVb (ST515, CC1). The strains from environmental swabs belonged, exclusively, to ST21 and were isolated from cutting board and floor swabs in 2011. Furthermore, we found Tn6188, a novel transposon conferring tolerance to BC, to be specific to sequence type ST121. In addition, antimicrobial resistance genes mprF and fosX were present in clonal complexes CC21 and CC121, while complexes CC8 and CC1 exclusively harbored the mprF antimicrobial resistance gene.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Food Microbiology",
title = "Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014",
volume = "79",
pages = "116-122",
doi = "10.1016/j.fm.2018.12.005"
}

Zuber, I., Lakićević, B., Pietzka, A., Milanov, D., Đorđević, V., Karabasil, N., Teodorović, V., Ruppitsch, W.,& Dimitrijević, M.. (2019). Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014. in Food Microbiology
Academic Press Ltd- Elsevier Science Ltd, London., 79, 116-122.
https://doi.org/10.1016/j.fm.2018.12.005

Zuber I, Lakićević B, Pietzka A, Milanov D, Đorđević V, Karabasil N, Teodorović V, Ruppitsch W, Dimitrijević M. Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014. in Food Microbiology. 2019;79:116-122.
doi:10.1016/j.fm.2018.12.005 .

Zuber, Ivana, Lakićević, Brankica, Pietzka, Ariane, Milanov, Dubravka, Đorđević, Vesna, Karabasil, Nedjeljko, Teodorović, Vlado, Ruppitsch, Werner, Dimitrijević, Mirjana, "Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014" in Food Microbiology, 79 (2019):116-122,
https://doi.org/10.1016/j.fm.2018.12.005 . .

TY  - JOUR
AU  - Zuber, Ivana
AU  - Lakićević, Brankica
AU  - Pietzka, Ariane
AU  - Milanov, Dubravka
AU  - Đorđević, Vesna
AU  - Karabasil, Nedjeljko
AU  - Teodorović, Vlado
AU  - Ruppitsch, Werner
AU  - Dimitrijević, Mirjana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1728
AB  - The presence of Listeria monocytogenes was evaluated in a small-scale meat processing facility in Montenegro during 2011-2014. L. monocytogenes isolates from traditional meat products and environmental swabs were subjected to a) molecular characterization b) serotyping by both multiplex PCR and next generation sequencing (NGS) c) potential antimicrobial resistance (AMR) was assessed by extraction of specific genes from NGS data and d) screening for the presence of some disinfectant resistance markers. Overall, traditional meat products were contaminated, most likely from incoming raw materials, with 4 major specific STs of L. monocytogenes (ST515, ST8, ST21, ST121) representing 4 clonal complexes (CC1, CC8, CC21, CC121) identified during the four-year period. These strains belonged to serogroup IIa which predominated, followed by IVb (ST515, CC1). The strains from environmental swabs belonged, exclusively, to ST21 and were isolated from cutting board and floor swabs in 2011. Furthermore, we found Tn6188, a novel transposon conferring tolerance to BC, to be specific to sequence type ST121. In addition, antimicrobial resistance genes mprF and fosX were present in clonal complexes CC21 and CC121, while complexes CC8 and CC1 exclusively harbored the mprF antimicrobial resistance gene.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Food Microbiology
T1  - Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014
VL  - 79
SP  - 116
EP  - 122
DO  - 10.1016/j.fm.2018.12.005
ER  - 

@article{
author = "Zuber, Ivana and Lakićević, Brankica and Pietzka, Ariane and Milanov, Dubravka and Đorđević, Vesna and Karabasil, Nedjeljko and Teodorović, Vlado and Ruppitsch, Werner and Dimitrijević, Mirjana",
year = "2019",
abstract = "The presence of Listeria monocytogenes was evaluated in a small-scale meat processing facility in Montenegro during 2011-2014. L. monocytogenes isolates from traditional meat products and environmental swabs were subjected to a) molecular characterization b) serotyping by both multiplex PCR and next generation sequencing (NGS) c) potential antimicrobial resistance (AMR) was assessed by extraction of specific genes from NGS data and d) screening for the presence of some disinfectant resistance markers. Overall, traditional meat products were contaminated, most likely from incoming raw materials, with 4 major specific STs of L. monocytogenes (ST515, ST8, ST21, ST121) representing 4 clonal complexes (CC1, CC8, CC21, CC121) identified during the four-year period. These strains belonged to serogroup IIa which predominated, followed by IVb (ST515, CC1). The strains from environmental swabs belonged, exclusively, to ST21 and were isolated from cutting board and floor swabs in 2011. Furthermore, we found Tn6188, a novel transposon conferring tolerance to BC, to be specific to sequence type ST121. In addition, antimicrobial resistance genes mprF and fosX were present in clonal complexes CC21 and CC121, while complexes CC8 and CC1 exclusively harbored the mprF antimicrobial resistance gene.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Food Microbiology",
title = "Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014",
volume = "79",
pages = "116-122",
doi = "10.1016/j.fm.2018.12.005"
}

Zuber, I., Lakićević, B., Pietzka, A., Milanov, D., Đorđević, V., Karabasil, N., Teodorović, V., Ruppitsch, W.,& Dimitrijević, M.. (2019). Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014. in Food Microbiology
Academic Press Ltd- Elsevier Science Ltd, London., 79, 116-122.
https://doi.org/10.1016/j.fm.2018.12.005

Zuber I, Lakićević B, Pietzka A, Milanov D, Đorđević V, Karabasil N, Teodorović V, Ruppitsch W, Dimitrijević M. Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014. in Food Microbiology. 2019;79:116-122.
doi:10.1016/j.fm.2018.12.005 .

Zuber, Ivana, Lakićević, Brankica, Pietzka, Ariane, Milanov, Dubravka, Đorđević, Vesna, Karabasil, Nedjeljko, Teodorović, Vlado, Ruppitsch, Werner, Dimitrijević, Mirjana, "Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014" in Food Microbiology, 79 (2019):116-122,
https://doi.org/10.1016/j.fm.2018.12.005 . .

TY  - JOUR
AU  - Lepsanović, Zorica
AU  - Đorđević, Vesna
AU  - Lakićević, Brankica
AU  - Savić, Dejana
AU  - Velebit, Branko
AU  - Josić, Dragana
AU  - Karabasil, Nedjeljko
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1659
AB  - Bacillus cereus, a ubiquitous human foodborne pathogen, can persist in different environment, including dry conditions. In this study, we explored the genetic diversity of B. cereus isolates from infant milk formulas. Out of 83 samples, B. cereus was confirmed in 26 (31.3%). Isolates were resistant to penicillin G and ampicillin (100%), trimethoprim-sulfamethoxazole (84.6%), and erythromycin (3.8%). Ent, hbl, and cer genes were detected in 21 (80.8%), 2 (7.7%), and 11 (42.3%) isolates, respectively. Randomly amplified polymorphic DNA (RAPD) analysis, using set of three primers, provided an overall genetic distance of 95% between B. cereus isolates. A group of seven emetic strains and another two enterotoxic strains were indistinguishable by RAPD, revealing their high clonality. In conclusion, we detected a high proportion of pathogenic B. cereus isolates in infant milk formulas. The majority of isolates harboring both cer and ent genes showed high clonality, pointing food processing environment as probable contamination source. Practical applicationsDetection of Bacillus cereus in powdered infant milk formulas, the prevalence of emetic and diarrhegenic genes, and especially the simultaneous presence of both types of genes in the same isolate, confirm the importance of B. cereus as foodborne pathogen. The consumption of contaminated formula may cause serious health problems in babies, which emphasizes the need to improve B. cereus monitoring and characterization. The clonal relationship between strains, revealed by RAPD analysis, may point to the source of infection and the corresponding prevention measures to improve food safety. Moreover, the identical RAPD profile of cer positive strains obtained with AG15 primer enables the application of this analysis in screening for emetic B. cereus isolates.
PB  - Wiley, Hoboken
T2  - Journal of Food Safety
T1  - Detection of toxin genes and randomly amplified polymorphic DNA typing of Bacillus cereus isolates from infant milk formulas
VL  - 38
IS  - 4
SP  - e12474
DO  - 10.1111/jfs.12474
ER  - 

@article{
author = "Lepsanović, Zorica and Đorđević, Vesna and Lakićević, Brankica and Savić, Dejana and Velebit, Branko and Josić, Dragana and Karabasil, Nedjeljko",
year = "2018",
abstract = "Bacillus cereus, a ubiquitous human foodborne pathogen, can persist in different environment, including dry conditions. In this study, we explored the genetic diversity of B. cereus isolates from infant milk formulas. Out of 83 samples, B. cereus was confirmed in 26 (31.3%). Isolates were resistant to penicillin G and ampicillin (100%), trimethoprim-sulfamethoxazole (84.6%), and erythromycin (3.8%). Ent, hbl, and cer genes were detected in 21 (80.8%), 2 (7.7%), and 11 (42.3%) isolates, respectively. Randomly amplified polymorphic DNA (RAPD) analysis, using set of three primers, provided an overall genetic distance of 95% between B. cereus isolates. A group of seven emetic strains and another two enterotoxic strains were indistinguishable by RAPD, revealing their high clonality. In conclusion, we detected a high proportion of pathogenic B. cereus isolates in infant milk formulas. The majority of isolates harboring both cer and ent genes showed high clonality, pointing food processing environment as probable contamination source. Practical applicationsDetection of Bacillus cereus in powdered infant milk formulas, the prevalence of emetic and diarrhegenic genes, and especially the simultaneous presence of both types of genes in the same isolate, confirm the importance of B. cereus as foodborne pathogen. The consumption of contaminated formula may cause serious health problems in babies, which emphasizes the need to improve B. cereus monitoring and characterization. The clonal relationship between strains, revealed by RAPD analysis, may point to the source of infection and the corresponding prevention measures to improve food safety. Moreover, the identical RAPD profile of cer positive strains obtained with AG15 primer enables the application of this analysis in screening for emetic B. cereus isolates.",
publisher = "Wiley, Hoboken",
journal = "Journal of Food Safety",
title = "Detection of toxin genes and randomly amplified polymorphic DNA typing of Bacillus cereus isolates from infant milk formulas",
volume = "38",
number = "4",
pages = "e12474",
doi = "10.1111/jfs.12474"
}

Lepsanović, Z., Đorđević, V., Lakićević, B., Savić, D., Velebit, B., Josić, D.,& Karabasil, N.. (2018). Detection of toxin genes and randomly amplified polymorphic DNA typing of Bacillus cereus isolates from infant milk formulas. in Journal of Food Safety
Wiley, Hoboken., 38(4), e12474.
https://doi.org/10.1111/jfs.12474

Lepsanović Z, Đorđević V, Lakićević B, Savić D, Velebit B, Josić D, Karabasil N. Detection of toxin genes and randomly amplified polymorphic DNA typing of Bacillus cereus isolates from infant milk formulas. in Journal of Food Safety. 2018;38(4):e12474.
doi:10.1111/jfs.12474 .

Lepsanović, Zorica, Đorđević, Vesna, Lakićević, Brankica, Savić, Dejana, Velebit, Branko, Josić, Dragana, Karabasil, Nedjeljko, "Detection of toxin genes and randomly amplified polymorphic DNA typing of Bacillus cereus isolates from infant milk formulas" in Journal of Food Safety, 38, no. 4 (2018):e12474,
https://doi.org/10.1111/jfs.12474 . .