TY  - JOUR
AU  - Vasiljević, Marko
AU  - Marinković, Darko
AU  - Milićević, Dragan
AU  - Pleadin, Jelka
AU  - Stefanović, Srđan
AU  - Trialović, Saša
AU  - Raj, Jog
AU  - Petrujkić, Branko
AU  - Trialović, Jelena Nedejković
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2230
PB  - MDPI
T2  - Toxins
T1  - Efficacy of a Modified Clinoptilolite Based Adsorbent in Reducing Detrimental Effects of Ochratoxin A in Laying Hens
VL  - 13
IS  - 7
SP  - 469
DO  - 10.3390/toxins13070469
ER  - 

@article{
author = "Vasiljević, Marko and Marinković, Darko and Milićević, Dragan and Pleadin, Jelka and Stefanović, Srđan and Trialović, Saša and Raj, Jog and Petrujkić, Branko and Trialović, Jelena Nedejković",
year = "2021",
publisher = "MDPI",
journal = "Toxins",
title = "Efficacy of a Modified Clinoptilolite Based Adsorbent in Reducing Detrimental Effects of Ochratoxin A in Laying Hens",
volume = "13",
number = "7",
pages = "469",
doi = "10.3390/toxins13070469"
}

Vasiljević, M., Marinković, D., Milićević, D., Pleadin, J., Stefanović, S., Trialović, S., Raj, J., Petrujkić, B.,& Trialović, J. N.. (2021). Efficacy of a Modified Clinoptilolite Based Adsorbent in Reducing Detrimental Effects of Ochratoxin A in Laying Hens. in Toxins
MDPI., 13(7), 469.
https://doi.org/10.3390/toxins13070469

Vasiljević M, Marinković D, Milićević D, Pleadin J, Stefanović S, Trialović S, Raj J, Petrujkić B, Trialović JN. Efficacy of a Modified Clinoptilolite Based Adsorbent in Reducing Detrimental Effects of Ochratoxin A in Laying Hens. in Toxins. 2021;13(7):469.
doi:10.3390/toxins13070469 .

Vasiljević, Marko, Marinković, Darko, Milićević, Dragan, Pleadin, Jelka, Stefanović, Srđan, Trialović, Saša, Raj, Jog, Petrujkić, Branko, Trialović, Jelena Nedejković, "Efficacy of a Modified Clinoptilolite Based Adsorbent in Reducing Detrimental Effects of Ochratoxin A in Laying Hens" in Toxins, 13, no. 7 (2021):469,
https://doi.org/10.3390/toxins13070469 . .

TY  - JOUR
AU  - Marjanović, Đorđe
AU  - Trailović, Saša M.
AU  - Milovanović, Mirjana
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2040
AB  - Resistance of parasitic nematodes to anthelmintic drugs is a growing problem in human and veterinary medicine. The molecular mechanisms by which nematodes become resistant are different, but certainly one of the possible processes involves changing the drug binding site on the specific receptor. The significance of changes in individual subtypes of nicotinic acetylcholine receptors (nAChRs) for the development of resistance has not been clarified in detail. This study investigates the interaction of antinematodal drugs, agonist of different types of nAChRs and carvacrol with gamma aminobutyric acid (GABA) on the contractions of parasitic nematode A. suum. In our study, GABA (3 μM) produced significant increase of contractile EC50 value for pyrantel, and nonsignificant for bephenium and morantel, from 8.44 to 28.11 nM, 0.62 to 0.96 µM, and 3.72 to 5.69 nM, respectively. On the other hand, the maximal contractile effect (Rmax) did not change in the presence of GABA. However, when A. summ muscle flaps were incubated with GABA 3 μM and carvacrol 100 μM, the EC50 value of pyrantel, bephenium, and morantel was increased significantly to 44.62 nM, 1.40 μM, and nonsignificantly to 7.94 nM, respectively. Furthermore, Rmax decreased by 70, 60, and 65%. Presented results indicate that the combined use of GABA receptor agonists and nicotinic receptor antagonists can effectively inhibit the neuromuscular system of nematodes, even when one of the nicotinic receptor subtypes is dysfunctional, due to the potential development of resistance.
PB  - Exeley, Inc.
T2  - Journal of Nematology
T1  - Interaction of agonists of a different subtype of the nAChR and carvacrol with GABA in Ascaris suum somatic muscle contractions
VL  - 53
SP  - e2021-022
DO  - 10.21307/jofnem-2021-022
ER  - 

@article{
author = "Marjanović, Đorđe and Trailović, Saša M. and Milovanović, Mirjana",
year = "2021",
abstract = "Resistance of parasitic nematodes to anthelmintic drugs is a growing problem in human and veterinary medicine. The molecular mechanisms by which nematodes become resistant are different, but certainly one of the possible processes involves changing the drug binding site on the specific receptor. The significance of changes in individual subtypes of nicotinic acetylcholine receptors (nAChRs) for the development of resistance has not been clarified in detail. This study investigates the interaction of antinematodal drugs, agonist of different types of nAChRs and carvacrol with gamma aminobutyric acid (GABA) on the contractions of parasitic nematode A. suum. In our study, GABA (3 μM) produced significant increase of contractile EC50 value for pyrantel, and nonsignificant for bephenium and morantel, from 8.44 to 28.11 nM, 0.62 to 0.96 µM, and 3.72 to 5.69 nM, respectively. On the other hand, the maximal contractile effect (Rmax) did not change in the presence of GABA. However, when A. summ muscle flaps were incubated with GABA 3 μM and carvacrol 100 μM, the EC50 value of pyrantel, bephenium, and morantel was increased significantly to 44.62 nM, 1.40 μM, and nonsignificantly to 7.94 nM, respectively. Furthermore, Rmax decreased by 70, 60, and 65%. Presented results indicate that the combined use of GABA receptor agonists and nicotinic receptor antagonists can effectively inhibit the neuromuscular system of nematodes, even when one of the nicotinic receptor subtypes is dysfunctional, due to the potential development of resistance.",
publisher = "Exeley, Inc.",
journal = "Journal of Nematology",
title = "Interaction of agonists of a different subtype of the nAChR and carvacrol with GABA in Ascaris suum somatic muscle contractions",
volume = "53",
pages = "e2021-022",
doi = "10.21307/jofnem-2021-022"
}

Marjanović, Đ., Trailović, S. M.,& Milovanović, M.. (2021). Interaction of agonists of a different subtype of the nAChR and carvacrol with GABA in Ascaris suum somatic muscle contractions. in Journal of Nematology
Exeley, Inc.., 53, e2021-022.
https://doi.org/10.21307/jofnem-2021-022

Marjanović Đ, Trailović SM, Milovanović M. Interaction of agonists of a different subtype of the nAChR and carvacrol with GABA in Ascaris suum somatic muscle contractions. in Journal of Nematology. 2021;53:e2021-022.
doi:10.21307/jofnem-2021-022 .

Marjanović, Đorđe, Trailović, Saša M., Milovanović, Mirjana, "Interaction of agonists of a different subtype of the nAChR and carvacrol with GABA in Ascaris suum somatic muscle contractions" in Journal of Nematology, 53 (2021):e2021-022,
https://doi.org/10.21307/jofnem-2021-022 . .

TY  - JOUR
AU  - Marjanović, Đorđe
AU  - Zdravković, Nemanja
AU  - Milovanović, Mirjana
AU  - Nedeljković-Trailović, Jelena
AU  - Robertson, Alan P.
AU  - Todorović, Zoran
AU  - Trailović, Saša
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1807
AB  - The neuromuscular system of parasitic nematodes has proven to be an efficient pharmacological target for antihelmintics. Some of the most frequently used antiparasitic drugs are agonists or antagonists of nicotinic acetylcholine receptors (nAChRs). The antinematodal mechanism of action of carvacrol involves the inhibition of parasite muscle contraction. We have examined the interaction of carvacrol with antinematodal drugs that are agonists of different subtypes of nAChRs and monepantel, which is a non-competitive antagonist of this receptor in A. suum. Additionally, we investigated the effect of carvacrol on the muscle type of nAChRs in the mammalian host. As orthosteric agonists of nAChR, pyrantel, morantel and befinijum lead to dose-dependent contractions of the neuromuscular preparation of Ascaris suum. Carvacrol 100 mu M decreased the E-max of pyrantel, morantel and bephenium by 29%, 39% and 12 %, 39 % and 12 % respectively. The EC50 ratio was 3.43, 2.95 and 2.47 for pyrantel, morantel and bephinium, respectively. Carvacrol 300 u mu M reduces the E-max of pyrantel, morantel and bephenium by 71%, 80% and 75 %, 80 % and 75 % respectively. The EC50 ratio for pyrantel, morantel and bephenium was 3.88, 3.19 and 4.83 respectively. Furthermore, carvacrol enhances the inhibitory effect of monepantel on A. mum contractions, which may have an effective clinical application. On the other hand, tested concentrations of carvacrol did not significantly affect the EFS-induced contractions of the rat diaphragm, indicating a lack of interaction with the postsynaptic nAChR at the muscle end plate in mammals, but the highest concentration (300 mu M) caused a clear tetanic fade. Carvacrol exhibited a time and dose-dependent effect on the Rota-rod performances of rats with a high value of the ED50 (421.6 mg/kg). In our research, carvacrol dominantly exhibited characteristics of a non-competitive antagonist of nAChR in A. suum, and enhances the inhibitory effect of monepantel. The combination of monepantel and carvacrol may be clinically very effective, and the carvacrol molecule itself can be used as a promising platform for the development of new anthelmintic drugs.
PB  - Elsevier, Amsterdam
T2  - Veterinary Parasitology
T1  - Carvacrol acts as a potent selective antagonist of different types of nicotinic acetylcholine receptors and enhances the effect of monepantel in the parasitic nematode Ascaris suum
VL  - 278
SP  - UNSP 109031
DO  - 10.1016/j.vetpar.2020.109031
ER  - 

@article{
author = "Marjanović, Đorđe and Zdravković, Nemanja and Milovanović, Mirjana and Nedeljković-Trailović, Jelena and Robertson, Alan P. and Todorović, Zoran and Trailović, Saša",
year = "2020",
abstract = "The neuromuscular system of parasitic nematodes has proven to be an efficient pharmacological target for antihelmintics. Some of the most frequently used antiparasitic drugs are agonists or antagonists of nicotinic acetylcholine receptors (nAChRs). The antinematodal mechanism of action of carvacrol involves the inhibition of parasite muscle contraction. We have examined the interaction of carvacrol with antinematodal drugs that are agonists of different subtypes of nAChRs and monepantel, which is a non-competitive antagonist of this receptor in A. suum. Additionally, we investigated the effect of carvacrol on the muscle type of nAChRs in the mammalian host. As orthosteric agonists of nAChR, pyrantel, morantel and befinijum lead to dose-dependent contractions of the neuromuscular preparation of Ascaris suum. Carvacrol 100 mu M decreased the E-max of pyrantel, morantel and bephenium by 29%, 39% and 12 %, 39 % and 12 % respectively. The EC50 ratio was 3.43, 2.95 and 2.47 for pyrantel, morantel and bephinium, respectively. Carvacrol 300 u mu M reduces the E-max of pyrantel, morantel and bephenium by 71%, 80% and 75 %, 80 % and 75 % respectively. The EC50 ratio for pyrantel, morantel and bephenium was 3.88, 3.19 and 4.83 respectively. Furthermore, carvacrol enhances the inhibitory effect of monepantel on A. mum contractions, which may have an effective clinical application. On the other hand, tested concentrations of carvacrol did not significantly affect the EFS-induced contractions of the rat diaphragm, indicating a lack of interaction with the postsynaptic nAChR at the muscle end plate in mammals, but the highest concentration (300 mu M) caused a clear tetanic fade. Carvacrol exhibited a time and dose-dependent effect on the Rota-rod performances of rats with a high value of the ED50 (421.6 mg/kg). In our research, carvacrol dominantly exhibited characteristics of a non-competitive antagonist of nAChR in A. suum, and enhances the inhibitory effect of monepantel. The combination of monepantel and carvacrol may be clinically very effective, and the carvacrol molecule itself can be used as a promising platform for the development of new anthelmintic drugs.",
publisher = "Elsevier, Amsterdam",
journal = "Veterinary Parasitology",
title = "Carvacrol acts as a potent selective antagonist of different types of nicotinic acetylcholine receptors and enhances the effect of monepantel in the parasitic nematode Ascaris suum",
volume = "278",
pages = "UNSP 109031",
doi = "10.1016/j.vetpar.2020.109031"
}

Marjanović, Đ., Zdravković, N., Milovanović, M., Nedeljković-Trailović, J., Robertson, A. P., Todorović, Z.,& Trailović, S.. (2020). Carvacrol acts as a potent selective antagonist of different types of nicotinic acetylcholine receptors and enhances the effect of monepantel in the parasitic nematode Ascaris suum. in Veterinary Parasitology
Elsevier, Amsterdam., 278, UNSP 109031.
https://doi.org/10.1016/j.vetpar.2020.109031

Marjanović Đ, Zdravković N, Milovanović M, Nedeljković-Trailović J, Robertson AP, Todorović Z, Trailović S. Carvacrol acts as a potent selective antagonist of different types of nicotinic acetylcholine receptors and enhances the effect of monepantel in the parasitic nematode Ascaris suum. in Veterinary Parasitology. 2020;278:UNSP 109031.
doi:10.1016/j.vetpar.2020.109031 .

Marjanović, Đorđe, Zdravković, Nemanja, Milovanović, Mirjana, Nedeljković-Trailović, Jelena, Robertson, Alan P., Todorović, Zoran, Trailović, Saša, "Carvacrol acts as a potent selective antagonist of different types of nicotinic acetylcholine receptors and enhances the effect of monepantel in the parasitic nematode Ascaris suum" in Veterinary Parasitology, 278 (2020):UNSP 109031,
https://doi.org/10.1016/j.vetpar.2020.109031 . .

TY  - JOUR
AU  - Rajković, Milan
AU  - Vučićević, Ivana
AU  - Vučićević, Miloš
AU  - Dosenović, Milan
AU  - Charvet, L. Claude
AU  - Resanović, Radmila
AU  - Trailović, Saša
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1696
AB  - We present a case of Ascaridia galli infection in laying hens on a farm in central Serbia. During the rearing period on litter, ascaridosis was diagnosed at 15 weeks of age by routine parasitological fecal examination. Pullets were treated with flubendazole for one week, and two weeks later the hens were moved to battery cages. The production results were within technological standards until the 23rd week and the medical health status was without any clinical symptoms. After that period weight loss began, the egg production dropped to 70% and eggs were of poor quality. Subsequently, severe feather pecking and an increase of mortality were reported. The postmortal examination showed severe anemia and several gross lesions in the liver, intestines, lungs, and kidneys. Different sizes of A. galli were found in the lumen of the duodenum and jejunum. Gross changes of the intestinal mucosa were present, such as a thickened intestinal wall with hemorrhagic spots, inflammation and necrotic patches. Histopathological examination showed marked changes in the intestines, liver and kidneys. All visible live parasites were collected and stored in Earles balanced salts, and females were used for in vitro susceptibility testing. Median lethal concentration (LC50) of piperazine, levamisole and carvacrol for A. galli was 119.7 mu M, 2.71 mu M and 3.26 mu M, were applied, respectively. Based on our results, it is likely that reinfection occurred after completed dehelmintization. In relation to the new circumstances and the regulation for laying hen welfare the deworming protocol should be changed in order to ensure successful dehelmintization. In order to prevent reinfection the treatment must be done at the end of the rearing period and thus be maximally effective.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Ascaridia galli infection in laying hens and the results of in vitro efficacy of levamisole, piperazine and carvacrol, whether is necessary to change the deworming protocols?
VL  - 69
IS  - 4
SP  - 414
EP  - 425
DO  - 10.2478/acve-2019-0035
ER  - 

@article{
author = "Rajković, Milan and Vučićević, Ivana and Vučićević, Miloš and Dosenović, Milan and Charvet, L. Claude and Resanović, Radmila and Trailović, Saša",
year = "2019",
abstract = "We present a case of Ascaridia galli infection in laying hens on a farm in central Serbia. During the rearing period on litter, ascaridosis was diagnosed at 15 weeks of age by routine parasitological fecal examination. Pullets were treated with flubendazole for one week, and two weeks later the hens were moved to battery cages. The production results were within technological standards until the 23rd week and the medical health status was without any clinical symptoms. After that period weight loss began, the egg production dropped to 70% and eggs were of poor quality. Subsequently, severe feather pecking and an increase of mortality were reported. The postmortal examination showed severe anemia and several gross lesions in the liver, intestines, lungs, and kidneys. Different sizes of A. galli were found in the lumen of the duodenum and jejunum. Gross changes of the intestinal mucosa were present, such as a thickened intestinal wall with hemorrhagic spots, inflammation and necrotic patches. Histopathological examination showed marked changes in the intestines, liver and kidneys. All visible live parasites were collected and stored in Earles balanced salts, and females were used for in vitro susceptibility testing. Median lethal concentration (LC50) of piperazine, levamisole and carvacrol for A. galli was 119.7 mu M, 2.71 mu M and 3.26 mu M, were applied, respectively. Based on our results, it is likely that reinfection occurred after completed dehelmintization. In relation to the new circumstances and the regulation for laying hen welfare the deworming protocol should be changed in order to ensure successful dehelmintization. In order to prevent reinfection the treatment must be done at the end of the rearing period and thus be maximally effective.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Ascaridia galli infection in laying hens and the results of in vitro efficacy of levamisole, piperazine and carvacrol, whether is necessary to change the deworming protocols?",
volume = "69",
number = "4",
pages = "414-425",
doi = "10.2478/acve-2019-0035"
}

Rajković, M., Vučićević, I., Vučićević, M., Dosenović, M., Charvet, L. C., Resanović, R.,& Trailović, S.. (2019). Ascaridia galli infection in laying hens and the results of in vitro efficacy of levamisole, piperazine and carvacrol, whether is necessary to change the deworming protocols?. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(4), 414-425.
https://doi.org/10.2478/acve-2019-0035

Rajković M, Vučićević I, Vučićević M, Dosenović M, Charvet LC, Resanović R, Trailović S. Ascaridia galli infection in laying hens and the results of in vitro efficacy of levamisole, piperazine and carvacrol, whether is necessary to change the deworming protocols?. in Acta Veterinaria-Beograd. 2019;69(4):414-425.
doi:10.2478/acve-2019-0035 .

Rajković, Milan, Vučićević, Ivana, Vučićević, Miloš, Dosenović, Milan, Charvet, L. Claude, Resanović, Radmila, Trailović, Saša, "Ascaridia galli infection in laying hens and the results of in vitro efficacy of levamisole, piperazine and carvacrol, whether is necessary to change the deworming protocols?" in Acta Veterinaria-Beograd, 69, no. 4 (2019):414-425,
https://doi.org/10.2478/acve-2019-0035 . .

TY  - THES
AU  - Marjanović, Đorđe
PY  - 2019
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=
UR  - http://nardus.mpn.gov.rs/123456789/11903
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/58
AB  - U ovoj doktorskoj disertaciji provereni su i analizirani mehanizmi kojima pojedini aktivni sastojci esencijalnih biljnih ulja deluju na neuromišićni preparat A. suum. Ispitan je mehanizam antinematodnog dejstva odabranih biljnih mono i diterpenoida na neuromišićnom preparatu nematode i dejstvo na različite podtipove nikotinskog receptora (L, N i B tip). Pored toga ispitan je uticaj i interakcija terpenoidnih aktivnih sastojaka biljnih ulja na GABA receptor parazitske nematode. Takođe, testirana je njihova interakcija sa antinematodnim lekom iz grupe aminoacidonitrila (monepantel). Proverena je selektivnost dejstva karvakrola, karveola i mentola na neuromišićnim preparatima sisara (izolovana dijafragma i ileum pacova) i ispitano je dejstvo karvakrola na neuromišićni sistem sisara (Rota-rod test na pacovima). Ispitan je i uticaj timola i karvakrola na preživljavanje i faringealno pumpanje nematode Caenorhabditis elegans kao i dozna i vremenska zavisnost ovog efekta.
PB  - Univerzitet u Beogradu, Fakultet veterinarske medicine
T1  - Investigation of the antinematodal mechanism of action of selected monotherpenoid and ditherpenoids active ingredients from plant essential oils
T1  - Ispitivanje mehanizama antinematodnog dejstva odabranih monoterpenoidnih i diterpenoidnih aktivnih sastojaka esencijalnih biljnih ulja
UR  - https://hdl.handle.net/21.15107/rcub_nardus_11903
ER  - 

@phdthesis{
author = "Marjanović, Đorđe",
year = "2019",
abstract = "U ovoj doktorskoj disertaciji provereni su i analizirani mehanizmi kojima pojedini aktivni sastojci esencijalnih biljnih ulja deluju na neuromišićni preparat A. suum. Ispitan je mehanizam antinematodnog dejstva odabranih biljnih mono i diterpenoida na neuromišićnom preparatu nematode i dejstvo na različite podtipove nikotinskog receptora (L, N i B tip). Pored toga ispitan je uticaj i interakcija terpenoidnih aktivnih sastojaka biljnih ulja na GABA receptor parazitske nematode. Takođe, testirana je njihova interakcija sa antinematodnim lekom iz grupe aminoacidonitrila (monepantel). Proverena je selektivnost dejstva karvakrola, karveola i mentola na neuromišićnim preparatima sisara (izolovana dijafragma i ileum pacova) i ispitano je dejstvo karvakrola na neuromišićni sistem sisara (Rota-rod test na pacovima). Ispitan je i uticaj timola i karvakrola na preživljavanje i faringealno pumpanje nematode Caenorhabditis elegans kao i dozna i vremenska zavisnost ovog efekta.",
publisher = "Univerzitet u Beogradu, Fakultet veterinarske medicine",
title = "Investigation of the antinematodal mechanism of action of selected monotherpenoid and ditherpenoids active ingredients from plant essential oils, Ispitivanje mehanizama antinematodnog dejstva odabranih monoterpenoidnih i diterpenoidnih aktivnih sastojaka esencijalnih biljnih ulja",
url = "https://hdl.handle.net/21.15107/rcub_nardus_11903"
}

Marjanović, Đ.. (2019). Investigation of the antinematodal mechanism of action of selected monotherpenoid and ditherpenoids active ingredients from plant essential oils. 
Univerzitet u Beogradu, Fakultet veterinarske medicine..
https://hdl.handle.net/21.15107/rcub_nardus_11903

Marjanović Đ. Investigation of the antinematodal mechanism of action of selected monotherpenoid and ditherpenoids active ingredients from plant essential oils. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_11903 .

Marjanović, Đorđe, "Investigation of the antinematodal mechanism of action of selected monotherpenoid and ditherpenoids active ingredients from plant essential oils" (2019),
https://hdl.handle.net/21.15107/rcub_nardus_11903 .

TY  - JOUR
AU  - Choudhary, Shivani
AU  - Marjanović, Đorđe
AU  - Wong, Colin R.
AU  - Zhang, Xiaoyu
AU  - Abongwa, Melanie
AU  - Coats, Joel R.
AU  - Trailović, Saša
AU  - Martin, Richard J.
AU  - Robertson, Alan P.
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1730
AB  - The ongoing and widespread emergence of resistance to the existing anti-nematodal pharmacopeia has made it imperative to develop new anthelminthic agents. Historically, plants have been important sources of therapeutic compounds and offer an alternative to synthetic drugs. Monoterpenoids are phytochemicals that have been shown to produce acute toxic effects in insects and nematodes. Previous studies have shown nicotinic acetylcholine receptors (nAChRs) to be possible targets for naturally occurring plant metabolites such as carvacrol and carveol. In this study we examined the effects of monoterpenoid compounds on a levamisole sensitive nAChR from Oesophagostomum dentatum and a nicotine sensitive nAChR from Ascaris suum. We expressed the receptors in Xenopus laevis oocytes and used two-electrode voltage-clamp to characterize the effect of various compounds on these cys-loop receptors. At 100 mu M the majority of these compounds acted as antagonists. Interestingly, further experiments revealed that both 0.1 mu M and 10 mu M menthol potentiated acetylcholine and levamisole responses in the levamisole sensitive receptor but not the nicotine sensitive receptor. We also investigated the effects of 0.1 mu M menthol on the contractility of A. suum somatic muscle strips. Menthol produced significant potentiation of peak contractions at each concentration of acetylcholine. The positive allosteric modulatory effects of menthol in both in vivo and in vitro experiments suggests menthol as a promising candidate for combination therapy with cholinergic anthelmintics.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Journal for Parasitology-Drugs and Drug Resistance
T1  - Menthol acts as a positive allosteric modulator on nematode levamisole sensitive nicotinic acetylcholine receptors
VL  - 9
SP  - 44
EP  - 53
DO  - 10.1016/j.ijpddr.2018.12.005
ER  - 

@article{
author = "Choudhary, Shivani and Marjanović, Đorđe and Wong, Colin R. and Zhang, Xiaoyu and Abongwa, Melanie and Coats, Joel R. and Trailović, Saša and Martin, Richard J. and Robertson, Alan P.",
year = "2019",
abstract = "The ongoing and widespread emergence of resistance to the existing anti-nematodal pharmacopeia has made it imperative to develop new anthelminthic agents. Historically, plants have been important sources of therapeutic compounds and offer an alternative to synthetic drugs. Monoterpenoids are phytochemicals that have been shown to produce acute toxic effects in insects and nematodes. Previous studies have shown nicotinic acetylcholine receptors (nAChRs) to be possible targets for naturally occurring plant metabolites such as carvacrol and carveol. In this study we examined the effects of monoterpenoid compounds on a levamisole sensitive nAChR from Oesophagostomum dentatum and a nicotine sensitive nAChR from Ascaris suum. We expressed the receptors in Xenopus laevis oocytes and used two-electrode voltage-clamp to characterize the effect of various compounds on these cys-loop receptors. At 100 mu M the majority of these compounds acted as antagonists. Interestingly, further experiments revealed that both 0.1 mu M and 10 mu M menthol potentiated acetylcholine and levamisole responses in the levamisole sensitive receptor but not the nicotine sensitive receptor. We also investigated the effects of 0.1 mu M menthol on the contractility of A. suum somatic muscle strips. Menthol produced significant potentiation of peak contractions at each concentration of acetylcholine. The positive allosteric modulatory effects of menthol in both in vivo and in vitro experiments suggests menthol as a promising candidate for combination therapy with cholinergic anthelmintics.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Journal for Parasitology-Drugs and Drug Resistance",
title = "Menthol acts as a positive allosteric modulator on nematode levamisole sensitive nicotinic acetylcholine receptors",
volume = "9",
pages = "44-53",
doi = "10.1016/j.ijpddr.2018.12.005"
}

Choudhary, S., Marjanović, Đ., Wong, C. R., Zhang, X., Abongwa, M., Coats, J. R., Trailović, S., Martin, R. J.,& Robertson, A. P.. (2019). Menthol acts as a positive allosteric modulator on nematode levamisole sensitive nicotinic acetylcholine receptors. in International Journal for Parasitology-Drugs and Drug Resistance
Elsevier Sci Ltd, Oxford., 9, 44-53.
https://doi.org/10.1016/j.ijpddr.2018.12.005

Choudhary S, Marjanović Đ, Wong CR, Zhang X, Abongwa M, Coats JR, Trailović S, Martin RJ, Robertson AP. Menthol acts as a positive allosteric modulator on nematode levamisole sensitive nicotinic acetylcholine receptors. in International Journal for Parasitology-Drugs and Drug Resistance. 2019;9:44-53.
doi:10.1016/j.ijpddr.2018.12.005 .

Choudhary, Shivani, Marjanović, Đorđe, Wong, Colin R., Zhang, Xiaoyu, Abongwa, Melanie, Coats, Joel R., Trailović, Saša, Martin, Richard J., Robertson, Alan P., "Menthol acts as a positive allosteric modulator on nematode levamisole sensitive nicotinic acetylcholine receptors" in International Journal for Parasitology-Drugs and Drug Resistance, 9 (2019):44-53,
https://doi.org/10.1016/j.ijpddr.2018.12.005 . .

TY  - JOUR
AU  - Marjanović, Đorđe
AU  - Bogunović, Danica
AU  - Milovanović, Mirjana
AU  - Marinković, Darko
AU  - Zdravković, Nemanja
AU  - Magaš, Vladimir
AU  - Trailović, Saša
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1615
AB  - In the present study we tested the dose andh time dependence of the antinematodal effects of carvacrol and tyhmol on Caenorabditis elegans, and the efficacy of carvacrol, thymol, p-cymene and cinnamaldehyde,which were administrated in the drinking water of rats naturally infected with the pinworm Syphada muris. The control treatment of the infected rats was carried out with piperazine. Thymol caused a dose and time-dependent mortality in adult C. elegans. The value of the Median Lethal Concentration (LC50) of thymol was 117.9nM after 24h and 62.89 nM after 48h of exposure. Carvacrol exhibited a higher antinematodal efficiency than thymol. The LC50 of carvacrol, after 24 hours of exposure, was 53.03 nM, while after 48 hours it was 33.83 nM. On the other hand, piperazine showed an extremely high efficacy against S. muris infection in rats. Piperazine, at a dose of 625 mg/kg bw, administered in drinking water continuously for 10 days, eliminates the infection completely. However, none of the investigated active ingredients of essential oils were effective against S. muris. The reason for the lack of efficiency may be due to their pharmacokinetic properties. A relatively low amount of, orally administered, active ingredients of essential oils reaches the distal segments of the gastrointestinal tract, where S. muris inhabits the gut (colon and cecum). The obtained results, on C. elegans, indicate a clear dose and time-dependent antinematodal effect of thymol and carvacrol. However, for clinical application, it is necessary to examine the efficacy of microencapsulated formulations with a controlled release of active ingredients of essential oils in certain parts of the gastrointestinal tract.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Antihelminic activity of carvacrol, thymol, cinnamaldehyde and p-cymen against the free-living nematode caenorhabditis elegans and rat pinworm syphacia muris
VL  - 68
IS  - 4
SP  - 445
EP  - 456
DO  - 10.2478/acve-2018-0036
ER  - 

@article{
author = "Marjanović, Đorđe and Bogunović, Danica and Milovanović, Mirjana and Marinković, Darko and Zdravković, Nemanja and Magaš, Vladimir and Trailović, Saša",
year = "2018",
abstract = "In the present study we tested the dose andh time dependence of the antinematodal effects of carvacrol and tyhmol on Caenorabditis elegans, and the efficacy of carvacrol, thymol, p-cymene and cinnamaldehyde,which were administrated in the drinking water of rats naturally infected with the pinworm Syphada muris. The control treatment of the infected rats was carried out with piperazine. Thymol caused a dose and time-dependent mortality in adult C. elegans. The value of the Median Lethal Concentration (LC50) of thymol was 117.9nM after 24h and 62.89 nM after 48h of exposure. Carvacrol exhibited a higher antinematodal efficiency than thymol. The LC50 of carvacrol, after 24 hours of exposure, was 53.03 nM, while after 48 hours it was 33.83 nM. On the other hand, piperazine showed an extremely high efficacy against S. muris infection in rats. Piperazine, at a dose of 625 mg/kg bw, administered in drinking water continuously for 10 days, eliminates the infection completely. However, none of the investigated active ingredients of essential oils were effective against S. muris. The reason for the lack of efficiency may be due to their pharmacokinetic properties. A relatively low amount of, orally administered, active ingredients of essential oils reaches the distal segments of the gastrointestinal tract, where S. muris inhabits the gut (colon and cecum). The obtained results, on C. elegans, indicate a clear dose and time-dependent antinematodal effect of thymol and carvacrol. However, for clinical application, it is necessary to examine the efficacy of microencapsulated formulations with a controlled release of active ingredients of essential oils in certain parts of the gastrointestinal tract.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Antihelminic activity of carvacrol, thymol, cinnamaldehyde and p-cymen against the free-living nematode caenorhabditis elegans and rat pinworm syphacia muris",
volume = "68",
number = "4",
pages = "445-456",
doi = "10.2478/acve-2018-0036"
}

Marjanović, Đ., Bogunović, D., Milovanović, M., Marinković, D., Zdravković, N., Magaš, V.,& Trailović, S.. (2018). Antihelminic activity of carvacrol, thymol, cinnamaldehyde and p-cymen against the free-living nematode caenorhabditis elegans and rat pinworm syphacia muris. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(4), 445-456.
https://doi.org/10.2478/acve-2018-0036

Marjanović Đ, Bogunović D, Milovanović M, Marinković D, Zdravković N, Magaš V, Trailović S. Antihelminic activity of carvacrol, thymol, cinnamaldehyde and p-cymen against the free-living nematode caenorhabditis elegans and rat pinworm syphacia muris. in Acta Veterinaria-Beograd. 2018;68(4):445-456.
doi:10.2478/acve-2018-0036 .

Marjanović, Đorđe, Bogunović, Danica, Milovanović, Mirjana, Marinković, Darko, Zdravković, Nemanja, Magaš, Vladimir, Trailović, Saša, "Antihelminic activity of carvacrol, thymol, cinnamaldehyde and p-cymen against the free-living nematode caenorhabditis elegans and rat pinworm syphacia muris" in Acta Veterinaria-Beograd, 68, no. 4 (2018):445-456,
https://doi.org/10.2478/acve-2018-0036 . .

TY  - JOUR
AU  - Trailović, Saša
AU  - Marjanović, Đorđe
AU  - Vidonja-Uzelac, Teodora
AU  - Milovanović, Mirjana
AU  - Nedeljković-Trailović, Jelena
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1468
AB  - Acute poisoning with OPs may lead to a range of neurological effects, which cannot be explained by AChE inhibition alone. Several OPs interact directly with cholinergic receptors in mammals, but such data does not exist for invertebrates. The aim of current study was to investigate the direct and indirect effects of diazinon on the contractions of rat ileum and to compare those effects on the nervemuscle preparation of the Ascaris suum. In the presence of increasing concentrations of diazinon (3, 10 and 30 nM), EFS-induced ileal contractions were increased significantly. In the same preparation, diazinon 3 nM, significantly increased contractions induced by EFS, but did not affect the contractions caused by 5MFI. Contrarily, 1 mu M of diazinon significantly and reversibly inhibited the EFS-induced ileal contractions. Diazinon exhibited competitive and non-competitive inhibitions of 5MFI induced contractions. The control EC50 of 5MFI was 2.48 mu M with R-max = 1.88 g. In the presence of diazinon, EC50 was 12.45 mu M, while R-max was reduced to 0.43 g. After washing, the EC50 and R-max values were again closer to the control level (3.80 mu M and 1.04 g). Diazinon 1 mu M did not inhibit Ascaris suum contractions caused by ACh, but it increased the R-max. Diazinon in our study exhibits two opposite effects on the motor activity of the ileum. In low nanomolar concentrations the dominat is its effect on AChE and the stimulation of contractions. Furthermore, in concentrations that approach micromolar values diazinon has a direct inhibitory effect on muscarinic receptors. The direct inhibitory effect of diazinon on A. suum contractions was not found.
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Two opposite dose-dependent effects of diazinon on the motor activity of the rat ileum
VL  - 112
SP  - 18
EP  - 25
DO  - 10.1016/j.rvsc.2017.01.002
ER  - 

@article{
author = "Trailović, Saša and Marjanović, Đorđe and Vidonja-Uzelac, Teodora and Milovanović, Mirjana and Nedeljković-Trailović, Jelena",
year = "2017",
abstract = "Acute poisoning with OPs may lead to a range of neurological effects, which cannot be explained by AChE inhibition alone. Several OPs interact directly with cholinergic receptors in mammals, but such data does not exist for invertebrates. The aim of current study was to investigate the direct and indirect effects of diazinon on the contractions of rat ileum and to compare those effects on the nervemuscle preparation of the Ascaris suum. In the presence of increasing concentrations of diazinon (3, 10 and 30 nM), EFS-induced ileal contractions were increased significantly. In the same preparation, diazinon 3 nM, significantly increased contractions induced by EFS, but did not affect the contractions caused by 5MFI. Contrarily, 1 mu M of diazinon significantly and reversibly inhibited the EFS-induced ileal contractions. Diazinon exhibited competitive and non-competitive inhibitions of 5MFI induced contractions. The control EC50 of 5MFI was 2.48 mu M with R-max = 1.88 g. In the presence of diazinon, EC50 was 12.45 mu M, while R-max was reduced to 0.43 g. After washing, the EC50 and R-max values were again closer to the control level (3.80 mu M and 1.04 g). Diazinon 1 mu M did not inhibit Ascaris suum contractions caused by ACh, but it increased the R-max. Diazinon in our study exhibits two opposite effects on the motor activity of the ileum. In low nanomolar concentrations the dominat is its effect on AChE and the stimulation of contractions. Furthermore, in concentrations that approach micromolar values diazinon has a direct inhibitory effect on muscarinic receptors. The direct inhibitory effect of diazinon on A. suum contractions was not found.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Two opposite dose-dependent effects of diazinon on the motor activity of the rat ileum",
volume = "112",
pages = "18-25",
doi = "10.1016/j.rvsc.2017.01.002"
}

Trailović, S., Marjanović, Đ., Vidonja-Uzelac, T., Milovanović, M.,& Nedeljković-Trailović, J.. (2017). Two opposite dose-dependent effects of diazinon on the motor activity of the rat ileum. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 112, 18-25.
https://doi.org/10.1016/j.rvsc.2017.01.002

Trailović S, Marjanović Đ, Vidonja-Uzelac T, Milovanović M, Nedeljković-Trailović J. Two opposite dose-dependent effects of diazinon on the motor activity of the rat ileum. in Research in Veterinary Science. 2017;112:18-25.
doi:10.1016/j.rvsc.2017.01.002 .

Trailović, Saša, Marjanović, Đorđe, Vidonja-Uzelac, Teodora, Milovanović, Mirjana, Nedeljković-Trailović, Jelena, "Two opposite dose-dependent effects of diazinon on the motor activity of the rat ileum" in Research in Veterinary Science, 112 (2017):18-25,
https://doi.org/10.1016/j.rvsc.2017.01.002 . .

TY  - JOUR
AU  - Bošnjak, Ivan
AU  - Zdravković, Nemanja
AU  - Čolović, Svetlana
AU  - Ranđelović, Slađana
AU  - Galić, Nataša
AU  - Radojičić, Marina
AU  - Šekler, Milanko
AU  - Aleksić-Kovačević, Sanja
AU  - Krnjaić, Dejan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1330
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Neglected zoonosis: The prevalence of Salmonella spp. in pet reptiles in Serbia
T1  - Zanemarene zoonoze - prevalencija Salmonella spp. kod reptila, kućnih ljubimaca, u Srbiji
VL  - 73
IS  - 10
SP  - 980
EP  - 982
DO  - 10.2298/VSP160809222B
ER  - 

@article{
author = "Bošnjak, Ivan and Zdravković, Nemanja and Čolović, Svetlana and Ranđelović, Slađana and Galić, Nataša and Radojičić, Marina and Šekler, Milanko and Aleksić-Kovačević, Sanja and Krnjaić, Dejan",
year = "2016",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Neglected zoonosis: The prevalence of Salmonella spp. in pet reptiles in Serbia, Zanemarene zoonoze - prevalencija Salmonella spp. kod reptila, kućnih ljubimaca, u Srbiji",
volume = "73",
number = "10",
pages = "980-982",
doi = "10.2298/VSP160809222B"
}

Bošnjak, I., Zdravković, N., Čolović, S., Ranđelović, S., Galić, N., Radojičić, M., Šekler, M., Aleksić-Kovačević, S.,& Krnjaić, D.. (2016). Neglected zoonosis: The prevalence of Salmonella spp. in pet reptiles in Serbia. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 73(10), 980-982.
https://doi.org/10.2298/VSP160809222B

Bošnjak I, Zdravković N, Čolović S, Ranđelović S, Galić N, Radojičić M, Šekler M, Aleksić-Kovačević S, Krnjaić D. Neglected zoonosis: The prevalence of Salmonella spp. in pet reptiles in Serbia. in Vojnosanitetski pregled. 2016;73(10):980-982.
doi:10.2298/VSP160809222B .

Bošnjak, Ivan, Zdravković, Nemanja, Čolović, Svetlana, Ranđelović, Slađana, Galić, Nataša, Radojičić, Marina, Šekler, Milanko, Aleksić-Kovačević, Sanja, Krnjaić, Dejan, "Neglected zoonosis: The prevalence of Salmonella spp. in pet reptiles in Serbia" in Vojnosanitetski pregled, 73, no. 10 (2016):980-982,
https://doi.org/10.2298/VSP160809222B . .

TY  - JOUR
AU  - Milovanović, Mirjana
AU  - Milosavljević, Miloš
AU  - Marjanović, Đorđe
AU  - Trailović, Saša
AU  - Vučinić, Marijana
AU  - Nedeljković-Trailović, Jelena
AU  - Marković, Maja
AU  - Đurđević, Dragan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1354
AB  - Carvacrol is a monoterpenic phenol and an active ingredient of the plant essential oils of the family Lamiaceae. We have investigated the analgesic effect of carvacrol, the possible dependence of the effect in relation to animal sex, and the impact of carvacrol on motor coordination in rats. Hyperalgesia was induced by formalin (1.5%), which was administered SC in the upper lip of rat. Hyperalgesia and effects of carvacrol and indomethacin were measured by using the orofacial formalin test. The influence on motor coordination in animals treated with carvacrol was investigated by using the rota-rod test. Carvacrol administered PO in pre-treatment (45 min. prior to formalin) at a single dose of 50, 75 and 100 mg /kg BW, in the male, 50 and 100 mg /kg BW, in female rats caused a dose-dependent antinociceptive effect. This effect of carvacrol was significantly higher (P lt 0.01, P lt 0.001) in male rats. Compared with indomethacin administered during pre-treatment (2 mg/kg, PO), carvacrol (100 mg/kg) exhibits significantly higher (P  lt 0.05 and P  lt 0.001) antinociceptive effect on formalininduced hyperalgesia in male rats. In the rota-rod test carvacrol did not disturb the motor coordination in male rats, nor the dose of carvacrol with clear antinociceptive properties exhibited depressive effect on the CNS of treated rats. Keeping in mind that the monoterpene carvacrol is of plant origin, with potentially less side effects and without residues, it is realistic to expect the possibility of its therapeutic use in the treatment of inflammatory pain in animals.
AB  - Karvakrol je monoterpenski fenol i sastavni je deo esencijalnih ulja biljaka iz familije Lamiaceae. Cilj ovog istraživanja bio je da se ispita analgetičko dejstvo karvakrola, moguće razlike u farmakološkom odgovoru između polova, i uticaj karvakrola na koordinaciju kretanja kod pacova. Hiperalgezija je izazvana formalinom (1,5%) koji je aplikovan s.c. u gornju usnu pacova. Hiperalgezija i efekti karvakrola i indometacina mereni su orofacijalnim formalinskim testom. Uticaj na motornu koordinaciju kod životinja tretiranih karvakrolom ispitali smo rota-rod testom. Karvakrol primenjen p.o. u pretretmanu (45 min. pre aplikacije formalina) u dozi od 50, 75 i 100 mg/kg t.m. kod mužjaka i dozi od 50 i 100 mg/kg t.m. kod ženki pacova, prouzrokuje dozno zavisan antinociceptivni efekat. Ovaj efekat karvakrola je značajno veći (P lt 0,01, P lt 0,001) kod pacova muškog pola. U poređenju sa indometacinom aplikovanim u pretretmanu (2 mg/kg, p.o.), karvakrol (100 mg/kg) ispoljava značajno veći (P lt 0,05 i P lt 0,001) antinociceptivni efekat na formalinom izazvanu hiperalgeziju kod mužjaka pacova. U rota-rod testu karvakrol nije narušio motornu koordinaciju kod mužjka pacova, niti doza karvakrol sa jasnim antinociceptivnim svojstvima deluje depresivno na CNS tretiranih pacova. Imajući u vidu da je karvakrol monoterpen biljnog porekla sa malo potencijalnih neželjenih dejstava i bez rezidua, realno je očekivati mogućnost njegove primenu u terapiji inflamatornog bola kod životinja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The effect of carvacrol on inflammatory pain and motor coordination in rats
T1  - Uticaj karvakrola na inflamatorni bol i koordinaciju kretanja kod pacova
VL  - 66
IS  - 4
SP  - 478
EP  - 488
DO  - 10.1515/acve-2016-0041
ER  - 

@article{
author = "Milovanović, Mirjana and Milosavljević, Miloš and Marjanović, Đorđe and Trailović, Saša and Vučinić, Marijana and Nedeljković-Trailović, Jelena and Marković, Maja and Đurđević, Dragan",
year = "2016",
abstract = "Carvacrol is a monoterpenic phenol and an active ingredient of the plant essential oils of the family Lamiaceae. We have investigated the analgesic effect of carvacrol, the possible dependence of the effect in relation to animal sex, and the impact of carvacrol on motor coordination in rats. Hyperalgesia was induced by formalin (1.5%), which was administered SC in the upper lip of rat. Hyperalgesia and effects of carvacrol and indomethacin were measured by using the orofacial formalin test. The influence on motor coordination in animals treated with carvacrol was investigated by using the rota-rod test. Carvacrol administered PO in pre-treatment (45 min. prior to formalin) at a single dose of 50, 75 and 100 mg /kg BW, in the male, 50 and 100 mg /kg BW, in female rats caused a dose-dependent antinociceptive effect. This effect of carvacrol was significantly higher (P lt 0.01, P lt 0.001) in male rats. Compared with indomethacin administered during pre-treatment (2 mg/kg, PO), carvacrol (100 mg/kg) exhibits significantly higher (P  lt 0.05 and P  lt 0.001) antinociceptive effect on formalininduced hyperalgesia in male rats. In the rota-rod test carvacrol did not disturb the motor coordination in male rats, nor the dose of carvacrol with clear antinociceptive properties exhibited depressive effect on the CNS of treated rats. Keeping in mind that the monoterpene carvacrol is of plant origin, with potentially less side effects and without residues, it is realistic to expect the possibility of its therapeutic use in the treatment of inflammatory pain in animals., Karvakrol je monoterpenski fenol i sastavni je deo esencijalnih ulja biljaka iz familije Lamiaceae. Cilj ovog istraživanja bio je da se ispita analgetičko dejstvo karvakrola, moguće razlike u farmakološkom odgovoru između polova, i uticaj karvakrola na koordinaciju kretanja kod pacova. Hiperalgezija je izazvana formalinom (1,5%) koji je aplikovan s.c. u gornju usnu pacova. Hiperalgezija i efekti karvakrola i indometacina mereni su orofacijalnim formalinskim testom. Uticaj na motornu koordinaciju kod životinja tretiranih karvakrolom ispitali smo rota-rod testom. Karvakrol primenjen p.o. u pretretmanu (45 min. pre aplikacije formalina) u dozi od 50, 75 i 100 mg/kg t.m. kod mužjaka i dozi od 50 i 100 mg/kg t.m. kod ženki pacova, prouzrokuje dozno zavisan antinociceptivni efekat. Ovaj efekat karvakrola je značajno veći (P lt 0,01, P lt 0,001) kod pacova muškog pola. U poređenju sa indometacinom aplikovanim u pretretmanu (2 mg/kg, p.o.), karvakrol (100 mg/kg) ispoljava značajno veći (P lt 0,05 i P lt 0,001) antinociceptivni efekat na formalinom izazvanu hiperalgeziju kod mužjaka pacova. U rota-rod testu karvakrol nije narušio motornu koordinaciju kod mužjka pacova, niti doza karvakrol sa jasnim antinociceptivnim svojstvima deluje depresivno na CNS tretiranih pacova. Imajući u vidu da je karvakrol monoterpen biljnog porekla sa malo potencijalnih neželjenih dejstava i bez rezidua, realno je očekivati mogućnost njegove primenu u terapiji inflamatornog bola kod životinja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The effect of carvacrol on inflammatory pain and motor coordination in rats, Uticaj karvakrola na inflamatorni bol i koordinaciju kretanja kod pacova",
volume = "66",
number = "4",
pages = "478-488",
doi = "10.1515/acve-2016-0041"
}

Milovanović, M., Milosavljević, M., Marjanović, Đ., Trailović, S., Vučinić, M., Nedeljković-Trailović, J., Marković, M.,& Đurđević, D.. (2016). The effect of carvacrol on inflammatory pain and motor coordination in rats. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(4), 478-488.
https://doi.org/10.1515/acve-2016-0041

Milovanović M, Milosavljević M, Marjanović Đ, Trailović S, Vučinić M, Nedeljković-Trailović J, Marković M, Đurđević D. The effect of carvacrol on inflammatory pain and motor coordination in rats. in Acta Veterinaria-Beograd. 2016;66(4):478-488.
doi:10.1515/acve-2016-0041 .

Milovanović, Mirjana, Milosavljević, Miloš, Marjanović, Đorđe, Trailović, Saša, Vučinić, Marijana, Nedeljković-Trailović, Jelena, Marković, Maja, Đurđević, Dragan, "The effect of carvacrol on inflammatory pain and motor coordination in rats" in Acta Veterinaria-Beograd, 66, no. 4 (2016):478-488,
https://doi.org/10.1515/acve-2016-0041 . .

TY  - JOUR
AU  - Marinković, Darko
AU  - Ozvegy, Jozsef
AU  - Aničić, Milan
AU  - Vučićević, Ivana
AU  - Nešić, Slađan
AU  - Kukolj, Vladimir
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1357
AB  - Gastric dilatation and volvulus is a life-threatening condition characterized by rapid accumulation of food and gases that cause displacement and distension of the stomach. The large and giant, deep-chested breeds of dogs are at higher risk for developing the gastric dilatation and volvulus. Uncommonly, it can also develop in cats, but it is also described in free-range polar bears. A case of gastric dilatation and volvulus in a brown bear (Ursus arctos) is described in this paper. This case was characterized by lack of any previous symptoms, sudden death, as well as macroscopic findings during necropsy - twisted distended stomach, congested displaced spleen and necrotic gastric wall. According to the available data this is the first described case report of the gastric dilatation and volvulus in brown bear (Ursus arctos).
AB  - Dilatacija i volvulus želuca je stanje opasno po život koje se karakteriše brzim nakupljanjem hrane i gasova dovodeći do pomeranja i rastezanja želuca. Psi velikih i gigantskih rasa sa dubokim grudnim košem su pod većim rizikom za razvoj ovog poremećaja. Veoma retko, dilatacija i torzija želuca može nastati i kod mačaka, a opisan je i slučaj kod polarnog medveda. U ovom radu je prikazan slučaj dilatacije i volvulusa želuca kod mrkog medveda (Ursus arctos). Izostanak simptoma, iznenadno uginuće, kao i makroskopski nalaz tokom obdukcije - uvrnut, proširen želudac, izmeštena kongestirana slezina i nekrotičan zid želuca su glavne karakteristike slučaja. Prema raspoloživim podacima ovo je prvi prikaz slučaja dilatacije i volvulusa želuca kod mrkog medveda (Ursus arctos).
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Gastric dilatation and volvulus in brown bear (Ursus arctos)
T1  - Dilatacija i volvulus želuca kod mrkog medveda (Ursus arctos)
VL  - 66
IS  - 3
SP  - 422
EP  - 428
DO  - 10.1515/acve-2016-0036
ER  - 

@article{
author = "Marinković, Darko and Ozvegy, Jozsef and Aničić, Milan and Vučićević, Ivana and Nešić, Slađan and Kukolj, Vladimir",
year = "2016",
abstract = "Gastric dilatation and volvulus is a life-threatening condition characterized by rapid accumulation of food and gases that cause displacement and distension of the stomach. The large and giant, deep-chested breeds of dogs are at higher risk for developing the gastric dilatation and volvulus. Uncommonly, it can also develop in cats, but it is also described in free-range polar bears. A case of gastric dilatation and volvulus in a brown bear (Ursus arctos) is described in this paper. This case was characterized by lack of any previous symptoms, sudden death, as well as macroscopic findings during necropsy - twisted distended stomach, congested displaced spleen and necrotic gastric wall. According to the available data this is the first described case report of the gastric dilatation and volvulus in brown bear (Ursus arctos)., Dilatacija i volvulus želuca je stanje opasno po život koje se karakteriše brzim nakupljanjem hrane i gasova dovodeći do pomeranja i rastezanja želuca. Psi velikih i gigantskih rasa sa dubokim grudnim košem su pod većim rizikom za razvoj ovog poremećaja. Veoma retko, dilatacija i torzija želuca može nastati i kod mačaka, a opisan je i slučaj kod polarnog medveda. U ovom radu je prikazan slučaj dilatacije i volvulusa želuca kod mrkog medveda (Ursus arctos). Izostanak simptoma, iznenadno uginuće, kao i makroskopski nalaz tokom obdukcije - uvrnut, proširen želudac, izmeštena kongestirana slezina i nekrotičan zid želuca su glavne karakteristike slučaja. Prema raspoloživim podacima ovo je prvi prikaz slučaja dilatacije i volvulusa želuca kod mrkog medveda (Ursus arctos).",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Gastric dilatation and volvulus in brown bear (Ursus arctos), Dilatacija i volvulus želuca kod mrkog medveda (Ursus arctos)",
volume = "66",
number = "3",
pages = "422-428",
doi = "10.1515/acve-2016-0036"
}

Marinković, D., Ozvegy, J., Aničić, M., Vučićević, I., Nešić, S.,& Kukolj, V.. (2016). Gastric dilatation and volvulus in brown bear (Ursus arctos). in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(3), 422-428.
https://doi.org/10.1515/acve-2016-0036

Marinković D, Ozvegy J, Aničić M, Vučićević I, Nešić S, Kukolj V. Gastric dilatation and volvulus in brown bear (Ursus arctos). in Acta Veterinaria-Beograd. 2016;66(3):422-428.
doi:10.1515/acve-2016-0036 .

Marinković, Darko, Ozvegy, Jozsef, Aničić, Milan, Vučićević, Ivana, Nešić, Slađan, Kukolj, Vladimir, "Gastric dilatation and volvulus in brown bear (Ursus arctos)" in Acta Veterinaria-Beograd, 66, no. 3 (2016):422-428,
https://doi.org/10.1515/acve-2016-0036 . .

TY  - JOUR
AU  - Trailović, Saša
AU  - Sapundžić Zurovac, Zorana
AU  - Gruborović, S.
AU  - Marjanović, Đorđe
AU  - Nedeljković-Trailović, Jelena
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1425
AB  - The aim of this study was to determine the role in contractions of postsynaptic nicotinic acetylcholine (nACh) and gamma- aminobutyric acid (GABA) receptors, in the bag region of Ascaris suum muscle cells, as well as the role of synaptic receptors between interneurons and motor neurons in the dorsal and ventral nerve cord. We have measured the isometric contractions of isolated segments of A. suum, with or without the nerve cord (dorsal or ventral). Contractions were caused by increasing concentrations of ACh or by electrical field stimulation (EFS). Based on our results, the presence of the nerve cord is essential for the contractile effects of ACh. The EC50 value of ACh for innervated muscle strips was 10.88 mu M. Unlike intact (innervated) preparations, there was no contraction of the muscle flaps when the nerve cord was mechanically removed. Furthermore, continuous EFS produced stable contractions of innervated muscle strips, but they are not sensitive to mecamylamine (100 mu M). However, GABA (30 mu M) significantly inhibited the EFS- induced contractions. EFS with the same characteristics did not cause muscle contractions of denervated muscle strips, but EFS with a wider pulse induced the increasing of tone and irregular contractions. These contractions were completely insensitive to the effect of GABA. The EC50 for ACh did not differ between the dorsal and ventral segments (9.83 mu M and 9.45 mu M), while GABA exhibited features of competitive and noncompetitive antagonists, regardless of whether it acted on the dorsal or ventral segments of A. suum. It is obvious that drugs will be more effective if they act on both the synaptic and extrasynaptic nACh and GABA receptors.
PB  - Cambridge Univ Press, Cambridge
T2  - Journal of Helminthology
T1  - Presynaptic and postsynaptic regulation of muscle contractions in the ascarid nematode Ascaris suum: a target for drug action
VL  - 90
IS  - 6
SP  - 698
EP  - 705
DO  - 10.1017/S0022149X15000978
ER  - 

@article{
author = "Trailović, Saša and Sapundžić Zurovac, Zorana and Gruborović, S. and Marjanović, Đorđe and Nedeljković-Trailović, Jelena",
year = "2016",
abstract = "The aim of this study was to determine the role in contractions of postsynaptic nicotinic acetylcholine (nACh) and gamma- aminobutyric acid (GABA) receptors, in the bag region of Ascaris suum muscle cells, as well as the role of synaptic receptors between interneurons and motor neurons in the dorsal and ventral nerve cord. We have measured the isometric contractions of isolated segments of A. suum, with or without the nerve cord (dorsal or ventral). Contractions were caused by increasing concentrations of ACh or by electrical field stimulation (EFS). Based on our results, the presence of the nerve cord is essential for the contractile effects of ACh. The EC50 value of ACh for innervated muscle strips was 10.88 mu M. Unlike intact (innervated) preparations, there was no contraction of the muscle flaps when the nerve cord was mechanically removed. Furthermore, continuous EFS produced stable contractions of innervated muscle strips, but they are not sensitive to mecamylamine (100 mu M). However, GABA (30 mu M) significantly inhibited the EFS- induced contractions. EFS with the same characteristics did not cause muscle contractions of denervated muscle strips, but EFS with a wider pulse induced the increasing of tone and irregular contractions. These contractions were completely insensitive to the effect of GABA. The EC50 for ACh did not differ between the dorsal and ventral segments (9.83 mu M and 9.45 mu M), while GABA exhibited features of competitive and noncompetitive antagonists, regardless of whether it acted on the dorsal or ventral segments of A. suum. It is obvious that drugs will be more effective if they act on both the synaptic and extrasynaptic nACh and GABA receptors.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Journal of Helminthology",
title = "Presynaptic and postsynaptic regulation of muscle contractions in the ascarid nematode Ascaris suum: a target for drug action",
volume = "90",
number = "6",
pages = "698-705",
doi = "10.1017/S0022149X15000978"
}

Trailović, S., Sapundžić Zurovac, Z., Gruborović, S., Marjanović, Đ.,& Nedeljković-Trailović, J.. (2016). Presynaptic and postsynaptic regulation of muscle contractions in the ascarid nematode Ascaris suum: a target for drug action. in Journal of Helminthology
Cambridge Univ Press, Cambridge., 90(6), 698-705.
https://doi.org/10.1017/S0022149X15000978

Trailović S, Sapundžić Zurovac Z, Gruborović S, Marjanović Đ, Nedeljković-Trailović J. Presynaptic and postsynaptic regulation of muscle contractions in the ascarid nematode Ascaris suum: a target for drug action. in Journal of Helminthology. 2016;90(6):698-705.
doi:10.1017/S0022149X15000978 .

Trailović, Saša, Sapundžić Zurovac, Zorana, Gruborović, S., Marjanović, Đorđe, Nedeljković-Trailović, Jelena, "Presynaptic and postsynaptic regulation of muscle contractions in the ascarid nematode Ascaris suum: a target for drug action" in Journal of Helminthology, 90, no. 6 (2016):698-705,
https://doi.org/10.1017/S0022149X15000978 . .

TY  - JOUR
AU  - Trailović, Saša
AU  - Marinković, Darko
AU  - Kulišić, Zoran
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1386
AB  - Giant liver fluke (Fascioloides magna) infection is an important health problem of cervids in southeastern Europe. We measured the prevalence and intensity of infection with F. magna in a fenced area near the Danube River in the South Backa District of Serbia. Parasitologic, pathomorphologic, and histopathologic examinations were conducted from November 2007 to February 2008, beginning with a population of 127 adult fallow deer (Dama dama). After a positive diagnosis, therapy with triclabendazole-medicated corn was applied. Deer were treated at four baiting stations, using medicated feed providing triclabendazole at an estimated dose of 10-14 mg/kg of body weight per deer. Treatment lasted for 7 d in early February 2008 and an additional 7 d 2 wk later. For the complete success of pharmacotherapy it was necessary to prevent any contact of deer with the snail intermediate host (Galba truncatula). Intervention in the habitat, removing grass and low vegetation, and draining ponds reduces the possibility of contact. Six months after the treatment, livers of hunted deer were reddish, with fibrous tracks; pigmentation and cysts in the parenchyma were surrounded by a fibrous capsule and their fecal samples contained no eggs of F. magna. Over the following years, livers of hunted deer were negative, and the last control cull in March 2015 confirmed complete absence of infection. We reconfirmed the presence of giant liver flukes in fallow deer in Serbia, apparently the result of natural spread across the Danube from Hungary and Croatia. We also report that the treatment of deer with triclabendazole-medicated corn is an effective method for administration of therapeutic doses of drug in semicaptive deer. Interventions in the environment are necessary to prevent recontact of deer with habitats used by the snail intermediate host, and enable the success of the therapy.
PB  - Wildlife Disease Assoc, Inc, Lawrence
T2  - Journal of Wildlife Diseases
T1  - Diagnosis and therapy of liver fluke (fascioloides magna) infection in fallow deer (dama dama) in Serbia
VL  - 52
IS  - 2
SP  - 319
EP  - 326
DO  - 10.7589/2015-07-194
ER  - 

@article{
author = "Trailović, Saša and Marinković, Darko and Kulišić, Zoran",
year = "2016",
abstract = "Giant liver fluke (Fascioloides magna) infection is an important health problem of cervids in southeastern Europe. We measured the prevalence and intensity of infection with F. magna in a fenced area near the Danube River in the South Backa District of Serbia. Parasitologic, pathomorphologic, and histopathologic examinations were conducted from November 2007 to February 2008, beginning with a population of 127 adult fallow deer (Dama dama). After a positive diagnosis, therapy with triclabendazole-medicated corn was applied. Deer were treated at four baiting stations, using medicated feed providing triclabendazole at an estimated dose of 10-14 mg/kg of body weight per deer. Treatment lasted for 7 d in early February 2008 and an additional 7 d 2 wk later. For the complete success of pharmacotherapy it was necessary to prevent any contact of deer with the snail intermediate host (Galba truncatula). Intervention in the habitat, removing grass and low vegetation, and draining ponds reduces the possibility of contact. Six months after the treatment, livers of hunted deer were reddish, with fibrous tracks; pigmentation and cysts in the parenchyma were surrounded by a fibrous capsule and their fecal samples contained no eggs of F. magna. Over the following years, livers of hunted deer were negative, and the last control cull in March 2015 confirmed complete absence of infection. We reconfirmed the presence of giant liver flukes in fallow deer in Serbia, apparently the result of natural spread across the Danube from Hungary and Croatia. We also report that the treatment of deer with triclabendazole-medicated corn is an effective method for administration of therapeutic doses of drug in semicaptive deer. Interventions in the environment are necessary to prevent recontact of deer with habitats used by the snail intermediate host, and enable the success of the therapy.",
publisher = "Wildlife Disease Assoc, Inc, Lawrence",
journal = "Journal of Wildlife Diseases",
title = "Diagnosis and therapy of liver fluke (fascioloides magna) infection in fallow deer (dama dama) in Serbia",
volume = "52",
number = "2",
pages = "319-326",
doi = "10.7589/2015-07-194"
}

Trailović, S., Marinković, D.,& Kulišić, Z.. (2016). Diagnosis and therapy of liver fluke (fascioloides magna) infection in fallow deer (dama dama) in Serbia. in Journal of Wildlife Diseases
Wildlife Disease Assoc, Inc, Lawrence., 52(2), 319-326.
https://doi.org/10.7589/2015-07-194

Trailović S, Marinković D, Kulišić Z. Diagnosis and therapy of liver fluke (fascioloides magna) infection in fallow deer (dama dama) in Serbia. in Journal of Wildlife Diseases. 2016;52(2):319-326.
doi:10.7589/2015-07-194 .

Trailović, Saša, Marinković, Darko, Kulišić, Zoran, "Diagnosis and therapy of liver fluke (fascioloides magna) infection in fallow deer (dama dama) in Serbia" in Journal of Wildlife Diseases, 52, no. 2 (2016):319-326,
https://doi.org/10.7589/2015-07-194 . .

TY  - CONF
AU  - Trailović, Saša
AU  - Marjanović, Đorđe
AU  - Tipton, James M.
AU  - Abongwa, Melanie
AU  - Zheng, Fudan
AU  - Choudhary, Shivani
AU  - Martin, Richard J.
AU  - Robertson, Alan P.
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3346
AB  - Zolvix ® is a recently introduced anthelmintic drench containing monepantel as the active ingredient. 
Monepantel has been demonstrated to act on deg-3/des-2 containing nAChRs in several nematodes as 
a positive allosteric modulator. The drug produces hypercontraction of nematode muscle. We 
investigated the effects of monepantel on muscle strips isolated form adult Ascaris suum. The drug did 
not significantly increase baseline tension when applied on its own. Contractions induced by 
acetylcholine and several cholininomimetic anthelmintics where antagonized by monepantel. Further 
investigation revealed that the antagonism was non-competitive in nature. We also investigated the 
effect of monepantel on nAChRs from O. dentatum heterologously expressed in Xenopus laevis 
oocytes. We investigated the effects of monepantel on OD3, a levamisole preferring nAChR subtype, 
OD4, a pyrantel preferring subtype and ACR-16 a nicotine preferring subtype. For each subtype tested 
monepantel applied in isolation produced no measureable currents, nor did it produce currents in the 
presence of choline. When monepantel was continuously applied it reduced the amplitude of 
acetylcholine induced currents in a concentration dependent manner for the three nAChR subtypes 
tested. In all three cases monepantel acted as a non-competitive antagonist on the expressed 
receptors. ACR-16 was particularly sensitive to monepantel inhibition. Our findings suggest that the 
mode of action of monepantel is more complex than previously described.
PB  - Society of Toxicology
C3  - Antihelmintics : From discovery to resistance II, Captain’s Room: Marina Village Center: San Diego, CA- Feb. 9 - 12, 2016
T1  - Effects of monepantel on nicotinic acetylcholine receptors from Ascaris suum and Oesophagostomum dentatum
SP  - 11
EP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3346
ER  - 

@conference{
author = "Trailović, Saša and Marjanović, Đorđe and Tipton, James M. and Abongwa, Melanie and Zheng, Fudan and Choudhary, Shivani and Martin, Richard J. and Robertson, Alan P.",
year = "2016",
abstract = "Zolvix ® is a recently introduced anthelmintic drench containing monepantel as the active ingredient. 
Monepantel has been demonstrated to act on deg-3/des-2 containing nAChRs in several nematodes as 
a positive allosteric modulator. The drug produces hypercontraction of nematode muscle. We 
investigated the effects of monepantel on muscle strips isolated form adult Ascaris suum. The drug did 
not significantly increase baseline tension when applied on its own. Contractions induced by 
acetylcholine and several cholininomimetic anthelmintics where antagonized by monepantel. Further 
investigation revealed that the antagonism was non-competitive in nature. We also investigated the 
effect of monepantel on nAChRs from O. dentatum heterologously expressed in Xenopus laevis 
oocytes. We investigated the effects of monepantel on OD3, a levamisole preferring nAChR subtype, 
OD4, a pyrantel preferring subtype and ACR-16 a nicotine preferring subtype. For each subtype tested 
monepantel applied in isolation produced no measureable currents, nor did it produce currents in the 
presence of choline. When monepantel was continuously applied it reduced the amplitude of 
acetylcholine induced currents in a concentration dependent manner for the three nAChR subtypes 
tested. In all three cases monepantel acted as a non-competitive antagonist on the expressed 
receptors. ACR-16 was particularly sensitive to monepantel inhibition. Our findings suggest that the 
mode of action of monepantel is more complex than previously described.",
publisher = "Society of Toxicology",
journal = "Antihelmintics : From discovery to resistance II, Captain’s Room: Marina Village Center: San Diego, CA- Feb. 9 - 12, 2016",
title = "Effects of monepantel on nicotinic acetylcholine receptors from Ascaris suum and Oesophagostomum dentatum",
pages = "11-11",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3346"
}

Trailović, S., Marjanović, Đ., Tipton, J. M., Abongwa, M., Zheng, F., Choudhary, S., Martin, R. J.,& Robertson, A. P.. (2016). Effects of monepantel on nicotinic acetylcholine receptors from Ascaris suum and Oesophagostomum dentatum. in Antihelmintics : From discovery to resistance II, Captain’s Room: Marina Village Center: San Diego, CA- Feb. 9 - 12, 2016
Society of Toxicology., 11-11.
https://hdl.handle.net/21.15107/rcub_veterinar_3346

Trailović S, Marjanović Đ, Tipton JM, Abongwa M, Zheng F, Choudhary S, Martin RJ, Robertson AP. Effects of monepantel on nicotinic acetylcholine receptors from Ascaris suum and Oesophagostomum dentatum. in Antihelmintics : From discovery to resistance II, Captain’s Room: Marina Village Center: San Diego, CA- Feb. 9 - 12, 2016. 2016;:11-11.
https://hdl.handle.net/21.15107/rcub_veterinar_3346 .

Trailović, Saša, Marjanović, Đorđe, Tipton, James M., Abongwa, Melanie, Zheng, Fudan, Choudhary, Shivani, Martin, Richard J., Robertson, Alan P., "Effects of monepantel on nicotinic acetylcholine receptors from Ascaris suum and Oesophagostomum dentatum" in Antihelmintics : From discovery to resistance II, Captain’s Room: Marina Village Center: San Diego, CA- Feb. 9 - 12, 2016 (2016):11-11,
https://hdl.handle.net/21.15107/rcub_veterinar_3346 .

TY  - JOUR
AU  - Ivanović, Saša
AU  - Dimitrijević, Blagoje
AU  - Ćupić, Vitomir
AU  - Jezdimirović, Milanka
AU  - Borozan, Sunčica
AU  - Savić, Mila
AU  - Savić, Đorđe
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1347
AB  - Diazinon (DZN) is an organophosphate insecticide which exerts its effect through the inhibition of acetyl cholinesterase enzyme (AChE). In this work, we studied the development of tolerance to subchronic p.o. administration of DZN in rats, under both in vivo and in vitro conditions. A group of 20 rats (2 groups, n = 10) was administered p.o. the 1/10 of established LD50 DZN (namely 55.87 mg/kg bw) for 28 days. On the 14th and 28th day of study with isolated diaphragm and ileum, we examined the downregulation of nicotinic and muscarinic receptor function through Electrical Field Stimulation (EFS). Maximum contractility of the diaphragm was recorded on the 14th day of the study (25% higher compared to the non-treated rats), while on the 28th day the contractions almost did not differ from the values found in non-treated rats. EFS of isolated ileum on the 14th day of study caused significantly higher contractions compared to the non treated rats, but after 28 days, ileum contractions decreased approximately to the level of contractions in non-treated rats. On the 14th study day, we also recorded increased amplitude of spontaneous ileum contractions, compared to non-treated rats. The application of increasing ACh concentrations caused dose-dependent ileum contractions, without statistically significant differences of median effective concentration (EC50) values in non-treated and treated rats. Tolerance to subchronic DZN administration develops due to various adaptation mechanisms, including the most important one downregulation of nicotinic and muscarinic receptor function.
PB  - Elsevier Science Bv, Amsterdam
T2  - Toxicology Reports
T1  - Downregulation of nicotinic and muscarinic receptor function in rats after subchronic exposure to diazinon
VL  - 3
SP  - 523
EP  - 530
DO  - 10.1016/j.toxrep.2016.06.002
ER  - 

@article{
author = "Ivanović, Saša and Dimitrijević, Blagoje and Ćupić, Vitomir and Jezdimirović, Milanka and Borozan, Sunčica and Savić, Mila and Savić, Đorđe",
year = "2016",
abstract = "Diazinon (DZN) is an organophosphate insecticide which exerts its effect through the inhibition of acetyl cholinesterase enzyme (AChE). In this work, we studied the development of tolerance to subchronic p.o. administration of DZN in rats, under both in vivo and in vitro conditions. A group of 20 rats (2 groups, n = 10) was administered p.o. the 1/10 of established LD50 DZN (namely 55.87 mg/kg bw) for 28 days. On the 14th and 28th day of study with isolated diaphragm and ileum, we examined the downregulation of nicotinic and muscarinic receptor function through Electrical Field Stimulation (EFS). Maximum contractility of the diaphragm was recorded on the 14th day of the study (25% higher compared to the non-treated rats), while on the 28th day the contractions almost did not differ from the values found in non-treated rats. EFS of isolated ileum on the 14th day of study caused significantly higher contractions compared to the non treated rats, but after 28 days, ileum contractions decreased approximately to the level of contractions in non-treated rats. On the 14th study day, we also recorded increased amplitude of spontaneous ileum contractions, compared to non-treated rats. The application of increasing ACh concentrations caused dose-dependent ileum contractions, without statistically significant differences of median effective concentration (EC50) values in non-treated and treated rats. Tolerance to subchronic DZN administration develops due to various adaptation mechanisms, including the most important one downregulation of nicotinic and muscarinic receptor function.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Toxicology Reports",
title = "Downregulation of nicotinic and muscarinic receptor function in rats after subchronic exposure to diazinon",
volume = "3",
pages = "523-530",
doi = "10.1016/j.toxrep.2016.06.002"
}

Ivanović, S., Dimitrijević, B., Ćupić, V., Jezdimirović, M., Borozan, S., Savić, M.,& Savić, Đ.. (2016). Downregulation of nicotinic and muscarinic receptor function in rats after subchronic exposure to diazinon. in Toxicology Reports
Elsevier Science Bv, Amsterdam., 3, 523-530.
https://doi.org/10.1016/j.toxrep.2016.06.002

Ivanović S, Dimitrijević B, Ćupić V, Jezdimirović M, Borozan S, Savić M, Savić Đ. Downregulation of nicotinic and muscarinic receptor function in rats after subchronic exposure to diazinon. in Toxicology Reports. 2016;3:523-530.
doi:10.1016/j.toxrep.2016.06.002 .

Ivanović, Saša, Dimitrijević, Blagoje, Ćupić, Vitomir, Jezdimirović, Milanka, Borozan, Sunčica, Savić, Mila, Savić, Đorđe, "Downregulation of nicotinic and muscarinic receptor function in rats after subchronic exposure to diazinon" in Toxicology Reports, 3 (2016):523-530,
https://doi.org/10.1016/j.toxrep.2016.06.002 . .

TY  - JOUR
AU  - Marinković, Darko
AU  - Milčić-Matić, Natalija
AU  - Jovanović, Milan
AU  - Vučićević, Ivana
AU  - Nešić, Slađan
AU  - Aničić, Milan
AU  - Aleksić-Kovačević, Sanja
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1199
AB  - Mast cell neoplasia in dogs can occur in two different forms: common as cutaneous tumor, or less common as a systemic form of neoplastic mast cell proliferation - systemic mastocytosis. The aim of this study was to compare the histological and cytological features, KIT receptor expression and presence of c-KIT proto-oncogene mutations in neoplastic cells of dogs with canine cutaneous mast cell tumor (CMCT) and systemic mastocytosis. Microscopical examination of the cytological smears obtained from all selected dogs revealed that cellular specimens were constituted mostly of round cells with a central nuclei and fine to coarse purple cytoplasmic granules. Histopathological examination of skin samples of dogs with CMCT and a dog with systemic mastocytosis showed proliferation of the neoplastic mast cells in the superficial and/or deep dermis. Similar findings were observed in tissue samples derived from lymph nodes, spleen, liver, myocardium and kidneys of a dog with systemic mastocytosis. Three dogs with high grade CMCT as well as one dog with systemic mastocytosis showed cytoplasmic CD117 expression, while 3 dogs with low grade CMCT, had membranous expression of CD117. Based on our study, histological features and cytoplasmic CD117 expression in neoplastic cells of dogs with systemic mastocytosis are similar to those in dogs with high grade CMCTs. Nevertheless, mutations of c-KIT proto-oncogene were not found in tumor samples either from dogs with CMCT or dog with systemic mastocytosis.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Morphological features and kit receptor expression in canine cutaneous mast cell tumor and systemic mastocytosis
VL  - 65
IS  - 2
SP  - 226
EP  - 237
DO  - 10.1515/acve-2015-0019
ER  - 

@article{
author = "Marinković, Darko and Milčić-Matić, Natalija and Jovanović, Milan and Vučićević, Ivana and Nešić, Slađan and Aničić, Milan and Aleksić-Kovačević, Sanja",
year = "2015",
abstract = "Mast cell neoplasia in dogs can occur in two different forms: common as cutaneous tumor, or less common as a systemic form of neoplastic mast cell proliferation - systemic mastocytosis. The aim of this study was to compare the histological and cytological features, KIT receptor expression and presence of c-KIT proto-oncogene mutations in neoplastic cells of dogs with canine cutaneous mast cell tumor (CMCT) and systemic mastocytosis. Microscopical examination of the cytological smears obtained from all selected dogs revealed that cellular specimens were constituted mostly of round cells with a central nuclei and fine to coarse purple cytoplasmic granules. Histopathological examination of skin samples of dogs with CMCT and a dog with systemic mastocytosis showed proliferation of the neoplastic mast cells in the superficial and/or deep dermis. Similar findings were observed in tissue samples derived from lymph nodes, spleen, liver, myocardium and kidneys of a dog with systemic mastocytosis. Three dogs with high grade CMCT as well as one dog with systemic mastocytosis showed cytoplasmic CD117 expression, while 3 dogs with low grade CMCT, had membranous expression of CD117. Based on our study, histological features and cytoplasmic CD117 expression in neoplastic cells of dogs with systemic mastocytosis are similar to those in dogs with high grade CMCTs. Nevertheless, mutations of c-KIT proto-oncogene were not found in tumor samples either from dogs with CMCT or dog with systemic mastocytosis.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Morphological features and kit receptor expression in canine cutaneous mast cell tumor and systemic mastocytosis",
volume = "65",
number = "2",
pages = "226-237",
doi = "10.1515/acve-2015-0019"
}

Marinković, D., Milčić-Matić, N., Jovanović, M., Vučićević, I., Nešić, S., Aničić, M.,& Aleksić-Kovačević, S.. (2015). Morphological features and kit receptor expression in canine cutaneous mast cell tumor and systemic mastocytosis. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(2), 226-237.
https://doi.org/10.1515/acve-2015-0019

Marinković D, Milčić-Matić N, Jovanović M, Vučićević I, Nešić S, Aničić M, Aleksić-Kovačević S. Morphological features and kit receptor expression in canine cutaneous mast cell tumor and systemic mastocytosis. in Acta Veterinaria-Beograd. 2015;65(2):226-237.
doi:10.1515/acve-2015-0019 .

Marinković, Darko, Milčić-Matić, Natalija, Jovanović, Milan, Vučićević, Ivana, Nešić, Slađan, Aničić, Milan, Aleksić-Kovačević, Sanja, "Morphological features and kit receptor expression in canine cutaneous mast cell tumor and systemic mastocytosis" in Acta Veterinaria-Beograd, 65, no. 2 (2015):226-237,
https://doi.org/10.1515/acve-2015-0019 . .

TY  - JOUR
AU  - Trailović, Saša
AU  - Marinković, Darko
AU  - Nedeljković-Trailović, Jelena
AU  - Milovanović, Mirjana
AU  - Marjanović, Đorđe
AU  - Aničić, Milan
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1314
AB  - Motility is required for feeding, reproduction and maintenance of the fluke in the hosts liver. According to that, the neuromuscular system can be an attractive drugable target for chemotherapy. Musculature of the Fascioloides magna is organized into three layers, an outer circular layer, beneath this layer the longitudinal layer, and third, the oblique, or diagonal layer underlies the longitudinal layer. In our study, the administration of atropine or caffeine did not cause classic muscle contractions of F. magna muscle strips. However, the Electrical Field Stimulation (EFS) induced stable and repeatable contractions, which enabled us to examine their sensitivity to the various substances. Acetylcholine (ACh) (300 mu M and 1 mM), caused only a slight relaxation, without affecting the amplitude of spontaneous contractions or the amplitude of contractions induced by EFS. Contrary to that, atropine (100 mu M) caused a significant increase in the basal tone and an increase of EFS-induced contractions. If acetylcholine is an inhibitory neurotransmitter in trematodes, the described effects of atropine are achieved by the blockade of inhibitory neurotransmission. On the other hand, with respect to the process of excitation-contraction coupling, the plant alkaloid ryanodine (30 mu M) significantly reduced the basal tone, as well as EFS-induced contractions of E magna muscle strips. Ryanodine inhibited the potentiating effect of atropine on the basal tone and contractions caused by EFS, which indicates that the contractile effect of atropine is dependent on Ca++ release from intracellular stores. Caffeine (500 mu M) caused relaxation of fluke muscle strips and at the same time significantly enhanced the EFS-induced contractions. Both effects of caffeine can be explained by entry of extracellular Ca++ into muscle cells. The muscle contractility of F. magna depends both on the entry of extracellular calcium, and calcium release from intracellular stores, which are under the control of RyRs. Our results also suggest that antitrematodal drugs could potentially be developed from substances with selective anti-cholinergic activity.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Experimental Parasitology
T1  - Pharmacological and morphological characteristics of the muscular system of the giant liver fluke (Fascioloides magna - Bassi 1875)
VL  - 159
SP  - 136
EP  - 142
DO  - 10.1016/j.exppara.2015.09.012
ER  - 

@article{
author = "Trailović, Saša and Marinković, Darko and Nedeljković-Trailović, Jelena and Milovanović, Mirjana and Marjanović, Đorđe and Aničić, Milan",
year = "2015",
abstract = "Motility is required for feeding, reproduction and maintenance of the fluke in the hosts liver. According to that, the neuromuscular system can be an attractive drugable target for chemotherapy. Musculature of the Fascioloides magna is organized into three layers, an outer circular layer, beneath this layer the longitudinal layer, and third, the oblique, or diagonal layer underlies the longitudinal layer. In our study, the administration of atropine or caffeine did not cause classic muscle contractions of F. magna muscle strips. However, the Electrical Field Stimulation (EFS) induced stable and repeatable contractions, which enabled us to examine their sensitivity to the various substances. Acetylcholine (ACh) (300 mu M and 1 mM), caused only a slight relaxation, without affecting the amplitude of spontaneous contractions or the amplitude of contractions induced by EFS. Contrary to that, atropine (100 mu M) caused a significant increase in the basal tone and an increase of EFS-induced contractions. If acetylcholine is an inhibitory neurotransmitter in trematodes, the described effects of atropine are achieved by the blockade of inhibitory neurotransmission. On the other hand, with respect to the process of excitation-contraction coupling, the plant alkaloid ryanodine (30 mu M) significantly reduced the basal tone, as well as EFS-induced contractions of E magna muscle strips. Ryanodine inhibited the potentiating effect of atropine on the basal tone and contractions caused by EFS, which indicates that the contractile effect of atropine is dependent on Ca++ release from intracellular stores. Caffeine (500 mu M) caused relaxation of fluke muscle strips and at the same time significantly enhanced the EFS-induced contractions. Both effects of caffeine can be explained by entry of extracellular Ca++ into muscle cells. The muscle contractility of F. magna depends both on the entry of extracellular calcium, and calcium release from intracellular stores, which are under the control of RyRs. Our results also suggest that antitrematodal drugs could potentially be developed from substances with selective anti-cholinergic activity.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Experimental Parasitology",
title = "Pharmacological and morphological characteristics of the muscular system of the giant liver fluke (Fascioloides magna - Bassi 1875)",
volume = "159",
pages = "136-142",
doi = "10.1016/j.exppara.2015.09.012"
}

Trailović, S., Marinković, D., Nedeljković-Trailović, J., Milovanović, M., Marjanović, Đ.,& Aničić, M.. (2015). Pharmacological and morphological characteristics of the muscular system of the giant liver fluke (Fascioloides magna - Bassi 1875). in Experimental Parasitology
Academic Press Inc Elsevier Science, San Diego., 159, 136-142.
https://doi.org/10.1016/j.exppara.2015.09.012

Trailović S, Marinković D, Nedeljković-Trailović J, Milovanović M, Marjanović Đ, Aničić M. Pharmacological and morphological characteristics of the muscular system of the giant liver fluke (Fascioloides magna - Bassi 1875). in Experimental Parasitology. 2015;159:136-142.
doi:10.1016/j.exppara.2015.09.012 .

Trailović, Saša, Marinković, Darko, Nedeljković-Trailović, Jelena, Milovanović, Mirjana, Marjanović, Đorđe, Aničić, Milan, "Pharmacological and morphological characteristics of the muscular system of the giant liver fluke (Fascioloides magna - Bassi 1875)" in Experimental Parasitology, 159 (2015):136-142,
https://doi.org/10.1016/j.exppara.2015.09.012 . .

TY  - JOUR
AU  - Nedeljković-Trailović, Jelena
AU  - Trailović, Saša
AU  - Resanović, Radmila
AU  - Milićević, Dragan
AU  - Jovanović, Milijan
AU  - Vasiljević, Marko
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1193
AB  - The aim of our study was to determine the efficacy of three different adsorbents, inorganic (modified zeolite), organic (esterified glucomannans) and mixed (inorganic and organic components, with the addition of enzymes), in protecting broilers from the toxic effects of ochratoxin A in feed. Broilers were fed diets containing 2 mg/kg of ochratoxin A (OTA) and supplemented with adsorbents at the recommended concentration of 2 g/kg for 21 days. The presence of OTA led to a notable reduction in body weight, lower weight gain, increased feed conversion and induced histopathological changes in the liver and kidneys. The presence of inorganic, organic and mixed adsorbents in contaminated feed only partially reduced the negative effects of OTA on the broiler performances. Broilers that were fed with adsorbent-supplemented feed reached higher body weight (17.96%, 19.09% and 13.59%), compared to the group that received only OTA. The presence of adsorbents partially alleviated the reduction in feed consumption (22.68%, 12.91% and 10.59%), and a similar effect was observed with feed conversion. The applied adsorbents have also reduced the intensity of histopathological changes caused by OTA; however, they were not able to prevent their onset. After the withdrawal of the toxin and adsorbents from the feed (21-42 days), all previously observed disturbances in broilers were reduced, but more remarkably in broilers fed with adsorbents.
PB  - MDPI, Basel
T2  - Toxins
T1  - Comparative Investigation of the Efficacy of Three Different Adsorbents against OTA-Induced Toxicity in Broiler Chickens
VL  - 7
IS  - 4
SP  - 1174
EP  - 1191
DO  - 10.3390/toxins7041174
ER  - 

@article{
author = "Nedeljković-Trailović, Jelena and Trailović, Saša and Resanović, Radmila and Milićević, Dragan and Jovanović, Milijan and Vasiljević, Marko",
year = "2015",
abstract = "The aim of our study was to determine the efficacy of three different adsorbents, inorganic (modified zeolite), organic (esterified glucomannans) and mixed (inorganic and organic components, with the addition of enzymes), in protecting broilers from the toxic effects of ochratoxin A in feed. Broilers were fed diets containing 2 mg/kg of ochratoxin A (OTA) and supplemented with adsorbents at the recommended concentration of 2 g/kg for 21 days. The presence of OTA led to a notable reduction in body weight, lower weight gain, increased feed conversion and induced histopathological changes in the liver and kidneys. The presence of inorganic, organic and mixed adsorbents in contaminated feed only partially reduced the negative effects of OTA on the broiler performances. Broilers that were fed with adsorbent-supplemented feed reached higher body weight (17.96%, 19.09% and 13.59%), compared to the group that received only OTA. The presence of adsorbents partially alleviated the reduction in feed consumption (22.68%, 12.91% and 10.59%), and a similar effect was observed with feed conversion. The applied adsorbents have also reduced the intensity of histopathological changes caused by OTA; however, they were not able to prevent their onset. After the withdrawal of the toxin and adsorbents from the feed (21-42 days), all previously observed disturbances in broilers were reduced, but more remarkably in broilers fed with adsorbents.",
publisher = "MDPI, Basel",
journal = "Toxins",
title = "Comparative Investigation of the Efficacy of Three Different Adsorbents against OTA-Induced Toxicity in Broiler Chickens",
volume = "7",
number = "4",
pages = "1174-1191",
doi = "10.3390/toxins7041174"
}

Nedeljković-Trailović, J., Trailović, S., Resanović, R., Milićević, D., Jovanović, M.,& Vasiljević, M.. (2015). Comparative Investigation of the Efficacy of Three Different Adsorbents against OTA-Induced Toxicity in Broiler Chickens. in Toxins
MDPI, Basel., 7(4), 1174-1191.
https://doi.org/10.3390/toxins7041174

Nedeljković-Trailović J, Trailović S, Resanović R, Milićević D, Jovanović M, Vasiljević M. Comparative Investigation of the Efficacy of Three Different Adsorbents against OTA-Induced Toxicity in Broiler Chickens. in Toxins. 2015;7(4):1174-1191.
doi:10.3390/toxins7041174 .

Nedeljković-Trailović, Jelena, Trailović, Saša, Resanović, Radmila, Milićević, Dragan, Jovanović, Milijan, Vasiljević, Marko, "Comparative Investigation of the Efficacy of Three Different Adsorbents against OTA-Induced Toxicity in Broiler Chickens" in Toxins, 7, no. 4 (2015):1174-1191,
https://doi.org/10.3390/toxins7041174 . .

TY  - JOUR
AU  - Trailović, Saša
AU  - Marjanović, Đorđe
AU  - Nedeljković-Trailović, Jelena
AU  - Robertson, Alan P.
AU  - Martin, Richard J.
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1290
AB  - Essential plant oils (or their active principles) are safe to use and a potentially attractive alternative to current antiparasitic drugs. In the present study, we tested the effects of carvacrol on the isolated tissues of Ascaris suum and investigated potential interactions with other antiparasitic drugs. We used somatic muscle flaps for contraction assays, as well as for electrophysiological investigations. Carvacrol 300 mu M highly significantly inhibited contractions caused by 1, 3, 10, 30, and 100 mu M of ACh (p = 0.0023, p = 0.0002, p = 0.0002, p < 0.0001, and p < 0.0001). The control EC50 for acetylcholine was 8.87 mu M (log EC50 = 0.95 +/- 0.26), while R (max) was 2.53 +/- 0.24 g. The EC50 of acetylcholine in the presence of 300 mu M of carvacrol was 27.71 mu M (log EC50 = 1.44 +/- 0.28) and the R (max) decreased to 1.63 +/- 0.32 g. Furthermore, carvacrol highly significant potentiates inhibitory effect of GABA and piperazine on the contractions induced by ACh. However, carvacrol (100 and 300 mu M), did not produce any changes in the membrane potential or conductance of the A. suum muscle cell. While, 300 mu M of carvacrol showed a significant inhibitory effect on ACh-induced depolarization response. The mean control depolarization was 13.58 +/- 0.66 mV and decreased in presence of carvacrol to 4.50 +/- 1.02 mV (p < 0.0001). Mean control Delta g was 0.168 +/- 0.017 mu S, while in the presence of 300 mu M of carvacrol, Delta g significantly decreased to 0.060 +/- 0.018 Delta S (p = 0.0017). The inhibitory effect on contractions may be the explanation of the antinematodal potential of carvacrol. Moreover, inhibition of depolarizations caused by ACh and reduction of conductance changes directly points to an interaction with the nAChR in A. suum.
PB  - Springer, New York
T2  - Parasitology Research
T1  - Interaction of carvacrol with the Ascaris suum nicotinic acetylcholine receptors and gamma-aminobutyric acid receptors, potential mechanism of antinematodal action
VL  - 114
IS  - 8
SP  - 3059
EP  - 3068
DO  - 10.1007/s00436-015-4508-x
ER  - 

@article{
author = "Trailović, Saša and Marjanović, Đorđe and Nedeljković-Trailović, Jelena and Robertson, Alan P. and Martin, Richard J.",
year = "2015",
abstract = "Essential plant oils (or their active principles) are safe to use and a potentially attractive alternative to current antiparasitic drugs. In the present study, we tested the effects of carvacrol on the isolated tissues of Ascaris suum and investigated potential interactions with other antiparasitic drugs. We used somatic muscle flaps for contraction assays, as well as for electrophysiological investigations. Carvacrol 300 mu M highly significantly inhibited contractions caused by 1, 3, 10, 30, and 100 mu M of ACh (p = 0.0023, p = 0.0002, p = 0.0002, p < 0.0001, and p < 0.0001). The control EC50 for acetylcholine was 8.87 mu M (log EC50 = 0.95 +/- 0.26), while R (max) was 2.53 +/- 0.24 g. The EC50 of acetylcholine in the presence of 300 mu M of carvacrol was 27.71 mu M (log EC50 = 1.44 +/- 0.28) and the R (max) decreased to 1.63 +/- 0.32 g. Furthermore, carvacrol highly significant potentiates inhibitory effect of GABA and piperazine on the contractions induced by ACh. However, carvacrol (100 and 300 mu M), did not produce any changes in the membrane potential or conductance of the A. suum muscle cell. While, 300 mu M of carvacrol showed a significant inhibitory effect on ACh-induced depolarization response. The mean control depolarization was 13.58 +/- 0.66 mV and decreased in presence of carvacrol to 4.50 +/- 1.02 mV (p < 0.0001). Mean control Delta g was 0.168 +/- 0.017 mu S, while in the presence of 300 mu M of carvacrol, Delta g significantly decreased to 0.060 +/- 0.018 Delta S (p = 0.0017). The inhibitory effect on contractions may be the explanation of the antinematodal potential of carvacrol. Moreover, inhibition of depolarizations caused by ACh and reduction of conductance changes directly points to an interaction with the nAChR in A. suum.",
publisher = "Springer, New York",
journal = "Parasitology Research",
title = "Interaction of carvacrol with the Ascaris suum nicotinic acetylcholine receptors and gamma-aminobutyric acid receptors, potential mechanism of antinematodal action",
volume = "114",
number = "8",
pages = "3059-3068",
doi = "10.1007/s00436-015-4508-x"
}

Trailović, S., Marjanović, Đ., Nedeljković-Trailović, J., Robertson, A. P.,& Martin, R. J.. (2015). Interaction of carvacrol with the Ascaris suum nicotinic acetylcholine receptors and gamma-aminobutyric acid receptors, potential mechanism of antinematodal action. in Parasitology Research
Springer, New York., 114(8), 3059-3068.
https://doi.org/10.1007/s00436-015-4508-x

Trailović S, Marjanović Đ, Nedeljković-Trailović J, Robertson AP, Martin RJ. Interaction of carvacrol with the Ascaris suum nicotinic acetylcholine receptors and gamma-aminobutyric acid receptors, potential mechanism of antinematodal action. in Parasitology Research. 2015;114(8):3059-3068.
doi:10.1007/s00436-015-4508-x .

Trailović, Saša, Marjanović, Đorđe, Nedeljković-Trailović, Jelena, Robertson, Alan P., Martin, Richard J., "Interaction of carvacrol with the Ascaris suum nicotinic acetylcholine receptors and gamma-aminobutyric acid receptors, potential mechanism of antinematodal action" in Parasitology Research, 114, no. 8 (2015):3059-3068,
https://doi.org/10.1007/s00436-015-4508-x . .

TY  - JOUR
AU  - Nedeljković-Trailović, Jelena
AU  - Trailović, Saša
AU  - Dimitrijević, Mirjana
AU  - Ilić, Vojislav
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1024
AB  - The 42-day long study was performed on a total number of 48 Hybro broilers divided into four groups. After the pre-experimental period of 14 days, 3 experimental groups of broilers (n=12) were formed and fed diets that contained 0.5, 1.0 and 1.5 mg/kg ochratoxin A (OTA) during the next seven consecutive days. In the same period, the control group of broilers was fed a diet with no toxin added. After the period of toxin addition, blood samples were taken from 6 animals in each group. The remaining animals (n=6) from the control and experimental groups were fed diets without OTA until the 42nd day of the study, when the blood samples were taken again. The total level of blood serum proteins was affected by treatment with different doses of OTA, but a significant and dose dependent increase of albumins together with a decrease of γ-globulin fraction was established. A/G ratio (Albumine/Globuline) suggested that the globulins were the dominant protein fraction in the blood serum samples obtained from all the broilers included in this study. The concentrations of α- and β- globulin in the serum were within physiological limits, but the concentration of γ-globulins significantly decreased. It can be concluded that the increasing dietary OTA levels (0.5, 1.0 and 1.5 mg/kg) had dose-dependent cumulative effect on blood serum proteins status in broilers, and the effect lasts even after the withdrawal of OTA from the feed.
AB  - Ispitivanje je trajalo 42 dan na ukupno 48 brojlera podeljenih u četiri jednake grupe. Preeksperimentalni period trajao je 14 dana, nakon čega su životinje tri ogledne grupe tokom sedam dana hranjene obrocma koji su sadržali 0,5, 1,0 i 1,5 mg ohratoksina A (OTA) u 1kg hrane. Brojleri kontrolne grupe u toku eksperimentalnog perioda hranjeni su hranom bez dodatka toksina. Posle sedam dana tretmana uzimani su uzorci krvi od po šest životinja iz svake grupe (kontrolne i eksperimentalnih). Preostale životinje hranjene su obrocima bez toksina do 42. dana ogleda, nakon čega je krv uzorkovana i od njih. Koncentracija ukupnih proteina plazme bila je promenjena u zavisnosti od koncentracije OTA koji su brojleri dobijali hranom. Takođe je zabeleženo značajno i dozno-zavisno povećanje koncentracije albumina sa snižavanjem koncentracije gama-globulinske frakcije u serumu. Albulin/Globulin odnos (A/G odnos) ukazuje da su globulini bili dominantna frakcija proteina kod svih brojlera u ispitivanju. Koncentracija alfa i beta-globulina u serumu brojlera kretala se u fiziološkim granicama dok je koncentracija gamaglobulina bila značajno snižena. Može se zaključiti da rastuće koncentracije OTA u hrani brojlera ispoljavaju dozno-zavistan kumulativni efekt na status serumskih proteina. Ovaj efekat ostaje i posle prestanka dodavanja toksina u hranu.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Blood serum protein status in broilers fed with increasing concentrations of ochratoxin A
T1  - Status serumskih proteina brojlera hranjenih obrocima sa rastućim koncentracijama ohratoksina A
VL  - 63
IS  - 1
SP  - 77
EP  - 88
DO  - 10.2298/AVB1301077N
ER  - 

@article{
author = "Nedeljković-Trailović, Jelena and Trailović, Saša and Dimitrijević, Mirjana and Ilić, Vojislav",
year = "2013",
abstract = "The 42-day long study was performed on a total number of 48 Hybro broilers divided into four groups. After the pre-experimental period of 14 days, 3 experimental groups of broilers (n=12) were formed and fed diets that contained 0.5, 1.0 and 1.5 mg/kg ochratoxin A (OTA) during the next seven consecutive days. In the same period, the control group of broilers was fed a diet with no toxin added. After the period of toxin addition, blood samples were taken from 6 animals in each group. The remaining animals (n=6) from the control and experimental groups were fed diets without OTA until the 42nd day of the study, when the blood samples were taken again. The total level of blood serum proteins was affected by treatment with different doses of OTA, but a significant and dose dependent increase of albumins together with a decrease of γ-globulin fraction was established. A/G ratio (Albumine/Globuline) suggested that the globulins were the dominant protein fraction in the blood serum samples obtained from all the broilers included in this study. The concentrations of α- and β- globulin in the serum were within physiological limits, but the concentration of γ-globulins significantly decreased. It can be concluded that the increasing dietary OTA levels (0.5, 1.0 and 1.5 mg/kg) had dose-dependent cumulative effect on blood serum proteins status in broilers, and the effect lasts even after the withdrawal of OTA from the feed., Ispitivanje je trajalo 42 dan na ukupno 48 brojlera podeljenih u četiri jednake grupe. Preeksperimentalni period trajao je 14 dana, nakon čega su životinje tri ogledne grupe tokom sedam dana hranjene obrocma koji su sadržali 0,5, 1,0 i 1,5 mg ohratoksina A (OTA) u 1kg hrane. Brojleri kontrolne grupe u toku eksperimentalnog perioda hranjeni su hranom bez dodatka toksina. Posle sedam dana tretmana uzimani su uzorci krvi od po šest životinja iz svake grupe (kontrolne i eksperimentalnih). Preostale životinje hranjene su obrocima bez toksina do 42. dana ogleda, nakon čega je krv uzorkovana i od njih. Koncentracija ukupnih proteina plazme bila je promenjena u zavisnosti od koncentracije OTA koji su brojleri dobijali hranom. Takođe je zabeleženo značajno i dozno-zavisno povećanje koncentracije albumina sa snižavanjem koncentracije gama-globulinske frakcije u serumu. Albulin/Globulin odnos (A/G odnos) ukazuje da su globulini bili dominantna frakcija proteina kod svih brojlera u ispitivanju. Koncentracija alfa i beta-globulina u serumu brojlera kretala se u fiziološkim granicama dok je koncentracija gamaglobulina bila značajno snižena. Može se zaključiti da rastuće koncentracije OTA u hrani brojlera ispoljavaju dozno-zavistan kumulativni efekt na status serumskih proteina. Ovaj efekat ostaje i posle prestanka dodavanja toksina u hranu.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Blood serum protein status in broilers fed with increasing concentrations of ochratoxin A, Status serumskih proteina brojlera hranjenih obrocima sa rastućim koncentracijama ohratoksina A",
volume = "63",
number = "1",
pages = "77-88",
doi = "10.2298/AVB1301077N"
}

Nedeljković-Trailović, J., Trailović, S., Dimitrijević, M.,& Ilić, V.. (2013). Blood serum protein status in broilers fed with increasing concentrations of ochratoxin A. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(1), 77-88.
https://doi.org/10.2298/AVB1301077N

Nedeljković-Trailović J, Trailović S, Dimitrijević M, Ilić V. Blood serum protein status in broilers fed with increasing concentrations of ochratoxin A. in Acta Veterinaria-Beograd. 2013;63(1):77-88.
doi:10.2298/AVB1301077N .

Nedeljković-Trailović, Jelena, Trailović, Saša, Dimitrijević, Mirjana, Ilić, Vojislav, "Blood serum protein status in broilers fed with increasing concentrations of ochratoxin A" in Acta Veterinaria-Beograd, 63, no. 1 (2013):77-88,
https://doi.org/10.2298/AVB1301077N . .

TY  - THES
AU  - Ivanović, Saša
PY  - 2013
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=44858895
UR  - http://nardus.mpn.gov.rs/123456789/2976
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/21
AB  - GABAergic and cholinergic systems in the neuromuscular system of the parasitic nematodes are the main target of action for anthelmintic drugs. In the nematode cholinergic system pharmacological importance has primarily nicotinic-acetylcholine receptor (nAChR). On the other hand, the GABA-receptor was originally identified as the main site of action for the Avermectins and Milbemicins. However, later was shown that these drugs work on a new, previously never described glutamate-gated chloride ion channel, located in the nematode pharynx. The most important problems that are endangering the success of antiparasitic therapy are the development of resistance in the parasites and often occurrences of the toxic effects of antiparasitic drugs in host. In the order to elucidate the mechanisms of action of GABAergičkih and cholinergic anthelmintic, we examined the pharmacological characteristics of the representatives of these two groups of drugs in the isolated nerve-muscle preparation of large pig nematode Ascaris suum. Also, it was important to examine comparatively differences in the effects of cholinergic and GABAergic anthelmintic on the corresponding receptors in mammals (investigations were performed on isolated rat diaphragm and ileum), and thus to analyze the mechanisms of their adverse effects. In presented investigations we measured the effects of contraction or relaxation on isolated nematode or mammalian preparations and analyzed the results by using appropriate statistical methods (non-linear regression, ANOVA, t-test). Based on the results obtained in our investigations the following conclusions may be presented: (1) Agonists of L, N and B type of nematode nicotinic acetylcholine receptor (nAChR) studied on a model of neuromuscular preparations of A. suum, showed variable efficacy. Highest efficiency in the first group of tested agonists has pyrantel (agonist of L-type nAChR, EC50=0.010 μM, Emax=2.5g), then bephenium (agonist of B-type nAChR, EC50=0.37μM, Emax=2.7g) and at the end acetylcholine (an endogenous neurotransmitter, agonists of all three types of receptors L, N and B, EC50=6.12-6.45μM, Emax=1.71-2.07g). Highest efficiency in the second group of nicotinic receptor agonists has been demonstrated by tribendimidine (probably agonist of L-type nAChR, EC50=0.064μM, Emax=1.29g), followed by levamisole (agonist of L-type nAChR, EC50=0.34μM, Emax=0.68g) and at the end nicotine (an agonist of N-type nAChR, EC50=4.99μM, Emax=1.07g). (2) Nicotinic acetylcholine receptor of A. suum, that acting tribendimidine and nicotine, exhibit characteristics of both types of mammalian nAChRs: a) the characteristics of mammalian muscle type of nAChR, because it is sensitive primarily to pancuronium but also to tubocurarine, and b) the characteristics of neuronal nAChRs of mammals, because it is sensitive primarily to mecamylamine but also to hexamethonium. (3) GABA induced a dose-dependent relaxation of A. suum neuromuscular preparation, the value of the mean EC50 was 7.40μM, while piperazine causes relaxation with EC50 of 331μM. Receptor through which GABA and piperazine exhibit relaxation of A. suum is different from mammalian GABAA receptor, because it is insensitive to bicuculine (specific mammalian GABAA receptor antagonist). (4) Ivermectin and moxidectin by themselves do not lead to the relaxation of neuromuscular preparation of A. suum. Furthermore, ivermectin does not potentiate the relaxation caused by GABA. Ivermectin induces relaxation only when is administered after GABA or in the neuromuscular preparations of A. suum with high amplitude of spontaneous activity (spontaneous contraction and relaxation about 0.5g). Therefore, ivermectin for its action requires prior activation of GABA-dependent chloride channels. (5) Carvacrol 100 and 300μM, causes long-term relaxation of neuromuscular preparation of A. suum. Carvacrol significantly potentiates relaxation caused by GABA and significantly inhibits the dose-dependent contractile effects of ACh, shifting the EC50 value from control 5.22μM to the 13.88μM and 22.72μM, respectively. Carvacrol reduced the maximum contractile effect of ACh from control 3.1g to the 2.50g and 1.96 g, respectively. Therefore, this is probably a classical non-competitive physiological antagonism, which carvacrol manifests by itself in relation to ACh. (6) Neither one combination of different parameters of Electrical Field Stimulation (EFS) (10Hz to 100Hz, 0.01-1.0ms, 5 to 30V, from 2.0 sec. to continuous stimulation), do not causes indirect stimulation of the A. suum neuromuscular preparation (contractions are insensitive to mecamylamine)...
AB  - Gabaergički i holinergički sistem u neuro-mišićnom sistemu parazitskih nematoda su glavna ciljna mesta delovanja antihelmintika. U okviru holinergičkog sistema nematoda, farmakološki značaj ima pre svega nikotinski-acetilholinski receptor (nAChR). S druge strane, GABA-receptor parazitskih nematoda je prvobitno označen kao glavno mesto dejstva avermektina i milbemicina. Međutim, vremenom se pokazalo da ovi lekovi deluju i na jedan potpuno nov, do tada neopisani glutamat-zavisni hloridni jonski kanal u farinksu nematoda. Pored toga, značajno je da neki aktivni principi etarskih ulja imaju dokazana antiparazitska svojstva, i da postoje indicije da deluju upravo preko ova dva receptorska sistema. Osnovni problemi koji danas ugrožavaju antiparazitsku terapiju su razvoj rezistencije i često ispoljavanje toksičnih efekata antiparazitskih lekova. Da bi se bolje razumeli mehanizmi dejstva gabaergičkih i holinergičkih antihelmintika ispitali smo farmakološke karakteristike predstavnika ove dve grupe lekova na neuro-mišićnom preparatu velike nematode svinja Ascaris suum. Takođe, značajno je bilo komparativno ispitati razlike u dejstvu gabaergičkih i holinergičkih antihelmintika na odgovarajuće receptore sisara (ispitivanja su izvršena na izolovanoj dijafragmi i ileumu pacova) i na taj način analizirati mehanizme njihovih neželjenih efekata. U našim istražvanjima mereni su efekti kontrakcije ili relaksacije izolovanih preparata i odgovarajućim statističkim metodama obrađivani dobijeni rezultati (nelinearna regresija, ANOVA, t-tets). Na osnovu rezultata dobijenih u ispitivanjima doneti su sledeći zaključci: (1) Agonisti L, N i B tipa nikotinskog-acetilholinskog receptora (nAChR) nematoda, ispitivani na modelu neuro-mišićnog preparata A. suum, ispoljili su različitu efikasnost. Najvišu efikasnost u prvoj grupi ispitivanih agonista, ispoljio je pirantel (agonista L-tipa nikotinskog receptora EC50=0.010μM, Emax=2.5g), zatim befinijum (agonista B-tipa nikotinskog receptora EC50=0.37μM, Emax=2.7g) i na kraju acetilholin (endogeni neurotransmiter), agonista sva tri tipa L, N i B nikotinskog receptora (EC50=6.12–6.45μM, Emax=1.71–2.07g). Najvišu efikasnost u drugoj grupi ispitivanih agonista nikotinskog receptora ispoljio je tribendimidin (najverovatnije agonista L-tipa nikotinskog receptora, EC50=0.064μM, Emax=1.29g), zatim levamizol (agonista L-tipa nikotinskog receptora, EC50=0.34μM, Emax=0.68g) i na kraju nikotin (agonista N-tipa nikotinskog receptora, EC50=4.99μM, Emax=1.07g). (2) Nikotinski-acetilholinski receptor A. suum, na koji deluju tribendimidin i nikotin ispoljava osobine oba tipa nAChR sisara: a) karakteristike mišićnog tipa nAChR sisara, jer je osetljiv pre svega na pankuronijum ali i na tubokurarin; b) karakteristike neuronskog tipa nAChR sisara, jer je osetljiv pre svega na mekamilamin ali i na heksametonijum. (3) GABA izaziva dozno-zavisnu relaksaciju neuro-mišićnog preparata A.suum, sa vrednošću srednje EC50 od 7.40μM, dok piperazin relaksaciju izaziva sa EC50 od 331μM. Receptor preko koga GABA i piperazin ostvaruju relaksaciju A. suum različit je od GABA receptora sisara jer je neosetljiv na bikukulin (specifični antagonista GABAA receptora). (4) Ivermektin i moksidektin sami po sebi ne dovode do relaksacije neuro-mišićnog preparata A. suum. Ivermektin ne potencira relaksaciju koju izaziva GABA. Međutim, ivermektin dovodi do relaksacije samo kada je aplikovan posle GABA ili na neuro-mišićnim preparatima A. suum sa velikom amplitudom spontane aktivnosti (spontane kontrakcije i relaksacije od oko 0.5g). Ivermektin za svoje dejstvo najverovatnije zahteva prethodnu aktivaciju GABA-zavisnog hloridnog kanala. (5) Karvakrol u koncentracijama od 100 i 300μM, izaziva dugotrajnu relaksaciju neuro-mišićnog preparata A. suum. Karvakrol signifikantno potencira relaksaciju koju izaziva GABA i signifikantno inhibiše dozno-zavisne kontrakcije izazvane sa ACh, pomerajuću vrednost EC50 ACh sa kontrolnih 5.22μM, na 13.88μM i 22.72μM. Karvakrol smanjuje maksimalni kontraktilni efekat ACh sa kontrolnih 3.1g na 2.50g i 1.96g. Ovde se verovatno radi o klasičnom nekompetitivnom fiziološkom antagonizmu, koji karvakrol ispoljava u odnosu na ACh. (6) Ni jedna kombinacija različitih parametara električne poljne stimulacije (EFS) (10 do 100Hz, 0.01-1.0ms, 5 do 30V, od 2.0s do kontinuirane stimulacije), ne izaziva indirektnu stimulaciju neuro-mišićnog preparata A. suum (kontrakcije su neosetljive na mekamilamin). Objašnjenje može biti veća osetljivost mišićne kontraktilne mašine na EFS u odnosu na nerve koji oslobađaju ACh. Kontrakcije A. suum dobijene sa EFS su ipak bile osetljive na 10 i 30μM GABA, što se može objasniti snažnom hiperpolarizacijom mišićnih i nervnih ćelija askarisa koju GABA izaziva...
PB  - Univerzitet u Beogradu, Fakultet veterinarske medicine
T1  - Comparative study of mechanism of antiparasitic and toxic action of gabaergic and cholinergic antihelmintics
T1  - Komparativno ispitivanje mehanizama antiparazitskog i toksičnog dejstva gabaergičkih i holinergičkih antihelmintika
UR  - https://hdl.handle.net/21.15107/rcub_nardus_2976
ER  - 

@phdthesis{
author = "Ivanović, Saša",
year = "2013",
abstract = "GABAergic and cholinergic systems in the neuromuscular system of the parasitic nematodes are the main target of action for anthelmintic drugs. In the nematode cholinergic system pharmacological importance has primarily nicotinic-acetylcholine receptor (nAChR). On the other hand, the GABA-receptor was originally identified as the main site of action for the Avermectins and Milbemicins. However, later was shown that these drugs work on a new, previously never described glutamate-gated chloride ion channel, located in the nematode pharynx. The most important problems that are endangering the success of antiparasitic therapy are the development of resistance in the parasites and often occurrences of the toxic effects of antiparasitic drugs in host. In the order to elucidate the mechanisms of action of GABAergičkih and cholinergic anthelmintic, we examined the pharmacological characteristics of the representatives of these two groups of drugs in the isolated nerve-muscle preparation of large pig nematode Ascaris suum. Also, it was important to examine comparatively differences in the effects of cholinergic and GABAergic anthelmintic on the corresponding receptors in mammals (investigations were performed on isolated rat diaphragm and ileum), and thus to analyze the mechanisms of their adverse effects. In presented investigations we measured the effects of contraction or relaxation on isolated nematode or mammalian preparations and analyzed the results by using appropriate statistical methods (non-linear regression, ANOVA, t-test). Based on the results obtained in our investigations the following conclusions may be presented: (1) Agonists of L, N and B type of nematode nicotinic acetylcholine receptor (nAChR) studied on a model of neuromuscular preparations of A. suum, showed variable efficacy. Highest efficiency in the first group of tested agonists has pyrantel (agonist of L-type nAChR, EC50=0.010 μM, Emax=2.5g), then bephenium (agonist of B-type nAChR, EC50=0.37μM, Emax=2.7g) and at the end acetylcholine (an endogenous neurotransmitter, agonists of all three types of receptors L, N and B, EC50=6.12-6.45μM, Emax=1.71-2.07g). Highest efficiency in the second group of nicotinic receptor agonists has been demonstrated by tribendimidine (probably agonist of L-type nAChR, EC50=0.064μM, Emax=1.29g), followed by levamisole (agonist of L-type nAChR, EC50=0.34μM, Emax=0.68g) and at the end nicotine (an agonist of N-type nAChR, EC50=4.99μM, Emax=1.07g). (2) Nicotinic acetylcholine receptor of A. suum, that acting tribendimidine and nicotine, exhibit characteristics of both types of mammalian nAChRs: a) the characteristics of mammalian muscle type of nAChR, because it is sensitive primarily to pancuronium but also to tubocurarine, and b) the characteristics of neuronal nAChRs of mammals, because it is sensitive primarily to mecamylamine but also to hexamethonium. (3) GABA induced a dose-dependent relaxation of A. suum neuromuscular preparation, the value of the mean EC50 was 7.40μM, while piperazine causes relaxation with EC50 of 331μM. Receptor through which GABA and piperazine exhibit relaxation of A. suum is different from mammalian GABAA receptor, because it is insensitive to bicuculine (specific mammalian GABAA receptor antagonist). (4) Ivermectin and moxidectin by themselves do not lead to the relaxation of neuromuscular preparation of A. suum. Furthermore, ivermectin does not potentiate the relaxation caused by GABA. Ivermectin induces relaxation only when is administered after GABA or in the neuromuscular preparations of A. suum with high amplitude of spontaneous activity (spontaneous contraction and relaxation about 0.5g). Therefore, ivermectin for its action requires prior activation of GABA-dependent chloride channels. (5) Carvacrol 100 and 300μM, causes long-term relaxation of neuromuscular preparation of A. suum. Carvacrol significantly potentiates relaxation caused by GABA and significantly inhibits the dose-dependent contractile effects of ACh, shifting the EC50 value from control 5.22μM to the 13.88μM and 22.72μM, respectively. Carvacrol reduced the maximum contractile effect of ACh from control 3.1g to the 2.50g and 1.96 g, respectively. Therefore, this is probably a classical non-competitive physiological antagonism, which carvacrol manifests by itself in relation to ACh. (6) Neither one combination of different parameters of Electrical Field Stimulation (EFS) (10Hz to 100Hz, 0.01-1.0ms, 5 to 30V, from 2.0 sec. to continuous stimulation), do not causes indirect stimulation of the A. suum neuromuscular preparation (contractions are insensitive to mecamylamine)..., Gabaergički i holinergički sistem u neuro-mišićnom sistemu parazitskih nematoda su glavna ciljna mesta delovanja antihelmintika. U okviru holinergičkog sistema nematoda, farmakološki značaj ima pre svega nikotinski-acetilholinski receptor (nAChR). S druge strane, GABA-receptor parazitskih nematoda je prvobitno označen kao glavno mesto dejstva avermektina i milbemicina. Međutim, vremenom se pokazalo da ovi lekovi deluju i na jedan potpuno nov, do tada neopisani glutamat-zavisni hloridni jonski kanal u farinksu nematoda. Pored toga, značajno je da neki aktivni principi etarskih ulja imaju dokazana antiparazitska svojstva, i da postoje indicije da deluju upravo preko ova dva receptorska sistema. Osnovni problemi koji danas ugrožavaju antiparazitsku terapiju su razvoj rezistencije i često ispoljavanje toksičnih efekata antiparazitskih lekova. Da bi se bolje razumeli mehanizmi dejstva gabaergičkih i holinergičkih antihelmintika ispitali smo farmakološke karakteristike predstavnika ove dve grupe lekova na neuro-mišićnom preparatu velike nematode svinja Ascaris suum. Takođe, značajno je bilo komparativno ispitati razlike u dejstvu gabaergičkih i holinergičkih antihelmintika na odgovarajuće receptore sisara (ispitivanja su izvršena na izolovanoj dijafragmi i ileumu pacova) i na taj način analizirati mehanizme njihovih neželjenih efekata. U našim istražvanjima mereni su efekti kontrakcije ili relaksacije izolovanih preparata i odgovarajućim statističkim metodama obrađivani dobijeni rezultati (nelinearna regresija, ANOVA, t-tets). Na osnovu rezultata dobijenih u ispitivanjima doneti su sledeći zaključci: (1) Agonisti L, N i B tipa nikotinskog-acetilholinskog receptora (nAChR) nematoda, ispitivani na modelu neuro-mišićnog preparata A. suum, ispoljili su različitu efikasnost. Najvišu efikasnost u prvoj grupi ispitivanih agonista, ispoljio je pirantel (agonista L-tipa nikotinskog receptora EC50=0.010μM, Emax=2.5g), zatim befinijum (agonista B-tipa nikotinskog receptora EC50=0.37μM, Emax=2.7g) i na kraju acetilholin (endogeni neurotransmiter), agonista sva tri tipa L, N i B nikotinskog receptora (EC50=6.12–6.45μM, Emax=1.71–2.07g). Najvišu efikasnost u drugoj grupi ispitivanih agonista nikotinskog receptora ispoljio je tribendimidin (najverovatnije agonista L-tipa nikotinskog receptora, EC50=0.064μM, Emax=1.29g), zatim levamizol (agonista L-tipa nikotinskog receptora, EC50=0.34μM, Emax=0.68g) i na kraju nikotin (agonista N-tipa nikotinskog receptora, EC50=4.99μM, Emax=1.07g). (2) Nikotinski-acetilholinski receptor A. suum, na koji deluju tribendimidin i nikotin ispoljava osobine oba tipa nAChR sisara: a) karakteristike mišićnog tipa nAChR sisara, jer je osetljiv pre svega na pankuronijum ali i na tubokurarin; b) karakteristike neuronskog tipa nAChR sisara, jer je osetljiv pre svega na mekamilamin ali i na heksametonijum. (3) GABA izaziva dozno-zavisnu relaksaciju neuro-mišićnog preparata A.suum, sa vrednošću srednje EC50 od 7.40μM, dok piperazin relaksaciju izaziva sa EC50 od 331μM. Receptor preko koga GABA i piperazin ostvaruju relaksaciju A. suum različit je od GABA receptora sisara jer je neosetljiv na bikukulin (specifični antagonista GABAA receptora). (4) Ivermektin i moksidektin sami po sebi ne dovode do relaksacije neuro-mišićnog preparata A. suum. Ivermektin ne potencira relaksaciju koju izaziva GABA. Međutim, ivermektin dovodi do relaksacije samo kada je aplikovan posle GABA ili na neuro-mišićnim preparatima A. suum sa velikom amplitudom spontane aktivnosti (spontane kontrakcije i relaksacije od oko 0.5g). Ivermektin za svoje dejstvo najverovatnije zahteva prethodnu aktivaciju GABA-zavisnog hloridnog kanala. (5) Karvakrol u koncentracijama od 100 i 300μM, izaziva dugotrajnu relaksaciju neuro-mišićnog preparata A. suum. Karvakrol signifikantno potencira relaksaciju koju izaziva GABA i signifikantno inhibiše dozno-zavisne kontrakcije izazvane sa ACh, pomerajuću vrednost EC50 ACh sa kontrolnih 5.22μM, na 13.88μM i 22.72μM. Karvakrol smanjuje maksimalni kontraktilni efekat ACh sa kontrolnih 3.1g na 2.50g i 1.96g. Ovde se verovatno radi o klasičnom nekompetitivnom fiziološkom antagonizmu, koji karvakrol ispoljava u odnosu na ACh. (6) Ni jedna kombinacija različitih parametara električne poljne stimulacije (EFS) (10 do 100Hz, 0.01-1.0ms, 5 do 30V, od 2.0s do kontinuirane stimulacije), ne izaziva indirektnu stimulaciju neuro-mišićnog preparata A. suum (kontrakcije su neosetljive na mekamilamin). Objašnjenje može biti veća osetljivost mišićne kontraktilne mašine na EFS u odnosu na nerve koji oslobađaju ACh. Kontrakcije A. suum dobijene sa EFS su ipak bile osetljive na 10 i 30μM GABA, što se može objasniti snažnom hiperpolarizacijom mišićnih i nervnih ćelija askarisa koju GABA izaziva...",
publisher = "Univerzitet u Beogradu, Fakultet veterinarske medicine",
title = "Comparative study of mechanism of antiparasitic and toxic action of gabaergic and cholinergic antihelmintics, Komparativno ispitivanje mehanizama antiparazitskog i toksičnog dejstva gabaergičkih i holinergičkih antihelmintika",
url = "https://hdl.handle.net/21.15107/rcub_nardus_2976"
}

Ivanović, S.. (2013). Comparative study of mechanism of antiparasitic and toxic action of gabaergic and cholinergic antihelmintics. 
Univerzitet u Beogradu, Fakultet veterinarske medicine..
https://hdl.handle.net/21.15107/rcub_nardus_2976

Ivanović S. Comparative study of mechanism of antiparasitic and toxic action of gabaergic and cholinergic antihelmintics. 2013;.
https://hdl.handle.net/21.15107/rcub_nardus_2976 .

Ivanović, Saša, "Comparative study of mechanism of antiparasitic and toxic action of gabaergic and cholinergic antihelmintics" (2013),
https://hdl.handle.net/21.15107/rcub_nardus_2976 .

TY  - JOUR
AU  - Vučinić, Marijana
AU  - Nedeljković-Trailović, Jelena
AU  - Trailović, Saša
AU  - Ivanović, Saša
AU  - Milovanović, Mirjana
AU  - Krnjaić, Dejan
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/883
AB  - The paper reviews the latest studies on possible applications of oregano essential oil in veterinary medicine and animal livestock production. The first part of the paper deals with the definition of essential oils, possibilities for their extraction from plants, possibilities for their application in human and veterinary medicine, the interest of a science in essential oils, and, essential oils classification based on their use in human and veterinary medicine. The second part of the review deals with the properties of oregano essential oil, its main active principles, carvacrol and thymol and its application in veterinary medicine and animal livestock production. Oregano essential oil may be applied in animal feed, in the treatment of coccidiosis of domestic animals and candidiasis. It can be applied as a larvicide, repellent, insecticide and acaricide. It is used in aquaculture to treat fish diseases caused by bacteria and parasites or in the hatchery industry as a disinfectant for eggs or for disinfection of manure. The greatest potential of oregano essential oil is the possibility of its application in organic agriculture and organic animal husbandry.
AB  - U radu su izložena novija saznanja o mogućnosti primene etarskog ulja origana u veterinarskoj medicini i stočarstvu. U prvom delu rada izneta je definicija i način dobijanja etarskih ulja, mogućnosti primene etarskih ulja, zainteresovanost nauke za etarska ulja, klasifikacija etarskih ulja na osnovu njihove primene u humanoj i veterinarskoj medicini. Drugi deo rada se odnosi na osobine etarskog ulja origana, njegove osnovne aktivne sastojke, karvakrol i timol, i mogućnost primene u veterinarskoj medicini i stočarstvu. Ulje origanaima široku mogućnost primene u ishrani domaćih životinja, u lečenju kokcidioze i kandidijaze domaćih životinja. Može da se primeni kao larvicid, repelent, insekticid i akaricid. Koristi se i u akvakulturi za lečenje bolesti riba prouzrokovanih bakterijama i parazitima, za dezinfekciju jaja i za dezinfekciju stajnjaka. Najveći potencijal etarskog ulja origana je mogućnost njegove primene u organskoj poljoprivredi i organskom stočarstvu.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Possibility for use essential oils in veterinary medicine and animal husbandry with special emphasis on oregano oil
T1  - Mogućnost primene etarskih ulja u veterinarskoj medicini i stočarstvu s posebnim osvrtom na etarsko ulje origana
VL  - 66
IS  - 5-6
SP  - 407
EP  - 416
DO  - 10.2298/VETGL1206407V
ER  - 

@article{
author = "Vučinić, Marijana and Nedeljković-Trailović, Jelena and Trailović, Saša and Ivanović, Saša and Milovanović, Mirjana and Krnjaić, Dejan",
year = "2012",
abstract = "The paper reviews the latest studies on possible applications of oregano essential oil in veterinary medicine and animal livestock production. The first part of the paper deals with the definition of essential oils, possibilities for their extraction from plants, possibilities for their application in human and veterinary medicine, the interest of a science in essential oils, and, essential oils classification based on their use in human and veterinary medicine. The second part of the review deals with the properties of oregano essential oil, its main active principles, carvacrol and thymol and its application in veterinary medicine and animal livestock production. Oregano essential oil may be applied in animal feed, in the treatment of coccidiosis of domestic animals and candidiasis. It can be applied as a larvicide, repellent, insecticide and acaricide. It is used in aquaculture to treat fish diseases caused by bacteria and parasites or in the hatchery industry as a disinfectant for eggs or for disinfection of manure. The greatest potential of oregano essential oil is the possibility of its application in organic agriculture and organic animal husbandry., U radu su izložena novija saznanja o mogućnosti primene etarskog ulja origana u veterinarskoj medicini i stočarstvu. U prvom delu rada izneta je definicija i način dobijanja etarskih ulja, mogućnosti primene etarskih ulja, zainteresovanost nauke za etarska ulja, klasifikacija etarskih ulja na osnovu njihove primene u humanoj i veterinarskoj medicini. Drugi deo rada se odnosi na osobine etarskog ulja origana, njegove osnovne aktivne sastojke, karvakrol i timol, i mogućnost primene u veterinarskoj medicini i stočarstvu. Ulje origanaima široku mogućnost primene u ishrani domaćih životinja, u lečenju kokcidioze i kandidijaze domaćih životinja. Može da se primeni kao larvicid, repelent, insekticid i akaricid. Koristi se i u akvakulturi za lečenje bolesti riba prouzrokovanih bakterijama i parazitima, za dezinfekciju jaja i za dezinfekciju stajnjaka. Najveći potencijal etarskog ulja origana je mogućnost njegove primene u organskoj poljoprivredi i organskom stočarstvu.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Possibility for use essential oils in veterinary medicine and animal husbandry with special emphasis on oregano oil, Mogućnost primene etarskih ulja u veterinarskoj medicini i stočarstvu s posebnim osvrtom na etarsko ulje origana",
volume = "66",
number = "5-6",
pages = "407-416",
doi = "10.2298/VETGL1206407V"
}

Vučinić, M., Nedeljković-Trailović, J., Trailović, S., Ivanović, S., Milovanović, M.,& Krnjaić, D.. (2012). Possibility for use essential oils in veterinary medicine and animal husbandry with special emphasis on oregano oil. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(5-6), 407-416.
https://doi.org/10.2298/VETGL1206407V

Vučinić M, Nedeljković-Trailović J, Trailović S, Ivanović S, Milovanović M, Krnjaić D. Possibility for use essential oils in veterinary medicine and animal husbandry with special emphasis on oregano oil. in Veterinarski Glasnik. 2012;66(5-6):407-416.
doi:10.2298/VETGL1206407V .

Vučinić, Marijana, Nedeljković-Trailović, Jelena, Trailović, Saša, Ivanović, Saša, Milovanović, Mirjana, Krnjaić, Dejan, "Possibility for use essential oils in veterinary medicine and animal husbandry with special emphasis on oregano oil" in Veterinarski Glasnik, 66, no. 5-6 (2012):407-416,
https://doi.org/10.2298/VETGL1206407V . .

TY  - JOUR
AU  - Petrujkić, Branko
AU  - Petrujkić, Tihomir
AU  - Šamanc, Horea
AU  - Trailović, Saša
AU  - Jeremić, Ivan
AU  - Nedeljković-Trailović, Jelena
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/931
AB  - Despite the fact that today different prevention programs are utilized, the incidence of puerperal metritis and endometritis occurrence has now significantly changed in last 30 years. The goal of this work was to evaluate cow endometritis treatments on the farm with the use of two local antiseptics (povidone iodine and chlorhexidine gluconate) and one antibiotic (cephapirine) and determine which of the treatments is economically most justified for field use in high producing dairy cows. The investigation was done on 90 Holstein-Friesian cows, from 3.5 to 5 old. Animals were checked for endometritis and randomly assigned to one of the groups. Endometritis treatments were performed from 28th till 35th day postpartum with cepharirine, hlorhexidine digluconate and povidon iodide. Highest fertility was achieved in the group of cows treated with cephapirine (83.33%). On the contrary, lowest fertility was determined in the group of cows treated wit 1% povidone iodine solution (76.67%), while the group of cows treated with 0.2% aqueous solution of chlorhexidine digluconate achieved fertility of 80.00%. Shorter service period was observed in the group of cows treated with cephapirine and chlorhexidine digluconate compared to the group of cows treated with % povidone iodine solution. Average number of insemination per successful conception in the group of cows treated with cephapirine and 0.2% chlorhexidine digluconate solution was lower than the one in group of cows treated with 1 % povidone iodine solution.
AB  - Iako se danas primenjuju različiti programi prevencije, incidenca pojavljivanja puerperalnih metritisa i endometritisa u poslednjih 30 godina se nije značajnije promenila. Cilj ovog rada bio je da se uporede rezultati lečenja endometritisa krava, primenom dva lokalna antiseptika (povidon jod i hlorheksidin diglukonat) i jednog antibiotika (cefapirin) i da se utvrdi koji je tretman ekonomski najopravdaniji. Ispitivanje je izvedeno na 90 krava Holštajn frizijske rase starosti 3,5 do 5 godina. Životinje su nakon izvršenog kliničkog pregleda i utvrđivanja statusa uterusa uvršćene u jednu od tri ogledne grupe. Lečenje endometritisa vršeno je u periodu od 28. do 35. dana post partum, preparatima cefapirina, povidon joda i hlorheksidin diglukonata. Najviša plodnost, ustanovljena je u grupi krava koja su tretirane cefapirinom i iznosila je 83,33%. Za razliku od toga, najniža plodnost utvrđena je u grupi krava koje su tretirane 1% povidonom 76,67%, nešto viša kod krava III ogledne grupe (tretirane 0,2% vodenim rastvorom hlorheksidin diglukonata) i iznosila je 80,00%. Servis period u grupi krava koje su tretirane cefapirinom i hlorheksidin diglukonatom je bio značajno kraći nego kod krava koje su tretirane 1% povidon jodom. Indeks osemenjavanja krava tretiranih cefapirinom ili 0,2% hlorheksidin diglukonatom niži je od indeksa osemenjavanja krava tretiranih 1% rastvorom povidon joda.
PB  - Institut PKB Agroekonomik, Padinska skela
T2  - Zbornik naučnih radova Instituta PKB Agroekonomik
T1  - Cephapirine, povidone iodine and chlorhexidine digluconate in dairy cows endometritis treatment
T1  - Cefapirin, povidon jod i hlorheksidin diglukonat u terapiji endometritisa visoko-mlečnih krava
VL  - 18
IS  - 3-4
SP  - 59
EP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_931
ER  - 

@article{
author = "Petrujkić, Branko and Petrujkić, Tihomir and Šamanc, Horea and Trailović, Saša and Jeremić, Ivan and Nedeljković-Trailović, Jelena",
year = "2012",
abstract = "Despite the fact that today different prevention programs are utilized, the incidence of puerperal metritis and endometritis occurrence has now significantly changed in last 30 years. The goal of this work was to evaluate cow endometritis treatments on the farm with the use of two local antiseptics (povidone iodine and chlorhexidine gluconate) and one antibiotic (cephapirine) and determine which of the treatments is economically most justified for field use in high producing dairy cows. The investigation was done on 90 Holstein-Friesian cows, from 3.5 to 5 old. Animals were checked for endometritis and randomly assigned to one of the groups. Endometritis treatments were performed from 28th till 35th day postpartum with cepharirine, hlorhexidine digluconate and povidon iodide. Highest fertility was achieved in the group of cows treated with cephapirine (83.33%). On the contrary, lowest fertility was determined in the group of cows treated wit 1% povidone iodine solution (76.67%), while the group of cows treated with 0.2% aqueous solution of chlorhexidine digluconate achieved fertility of 80.00%. Shorter service period was observed in the group of cows treated with cephapirine and chlorhexidine digluconate compared to the group of cows treated with % povidone iodine solution. Average number of insemination per successful conception in the group of cows treated with cephapirine and 0.2% chlorhexidine digluconate solution was lower than the one in group of cows treated with 1 % povidone iodine solution., Iako se danas primenjuju različiti programi prevencije, incidenca pojavljivanja puerperalnih metritisa i endometritisa u poslednjih 30 godina se nije značajnije promenila. Cilj ovog rada bio je da se uporede rezultati lečenja endometritisa krava, primenom dva lokalna antiseptika (povidon jod i hlorheksidin diglukonat) i jednog antibiotika (cefapirin) i da se utvrdi koji je tretman ekonomski najopravdaniji. Ispitivanje je izvedeno na 90 krava Holštajn frizijske rase starosti 3,5 do 5 godina. Životinje su nakon izvršenog kliničkog pregleda i utvrđivanja statusa uterusa uvršćene u jednu od tri ogledne grupe. Lečenje endometritisa vršeno je u periodu od 28. do 35. dana post partum, preparatima cefapirina, povidon joda i hlorheksidin diglukonata. Najviša plodnost, ustanovljena je u grupi krava koja su tretirane cefapirinom i iznosila je 83,33%. Za razliku od toga, najniža plodnost utvrđena je u grupi krava koje su tretirane 1% povidonom 76,67%, nešto viša kod krava III ogledne grupe (tretirane 0,2% vodenim rastvorom hlorheksidin diglukonata) i iznosila je 80,00%. Servis period u grupi krava koje su tretirane cefapirinom i hlorheksidin diglukonatom je bio značajno kraći nego kod krava koje su tretirane 1% povidon jodom. Indeks osemenjavanja krava tretiranih cefapirinom ili 0,2% hlorheksidin diglukonatom niži je od indeksa osemenjavanja krava tretiranih 1% rastvorom povidon joda.",
publisher = "Institut PKB Agroekonomik, Padinska skela",
journal = "Zbornik naučnih radova Instituta PKB Agroekonomik",
title = "Cephapirine, povidone iodine and chlorhexidine digluconate in dairy cows endometritis treatment, Cefapirin, povidon jod i hlorheksidin diglukonat u terapiji endometritisa visoko-mlečnih krava",
volume = "18",
number = "3-4",
pages = "59-70",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_931"
}

Petrujkić, B., Petrujkić, T., Šamanc, H., Trailović, S., Jeremić, I.,& Nedeljković-Trailović, J.. (2012). Cephapirine, povidone iodine and chlorhexidine digluconate in dairy cows endometritis treatment. in Zbornik naučnih radova Instituta PKB Agroekonomik
Institut PKB Agroekonomik, Padinska skela., 18(3-4), 59-70.
https://hdl.handle.net/21.15107/rcub_veterinar_931

Petrujkić B, Petrujkić T, Šamanc H, Trailović S, Jeremić I, Nedeljković-Trailović J. Cephapirine, povidone iodine and chlorhexidine digluconate in dairy cows endometritis treatment. in Zbornik naučnih radova Instituta PKB Agroekonomik. 2012;18(3-4):59-70.
https://hdl.handle.net/21.15107/rcub_veterinar_931 .

Petrujkić, Branko, Petrujkić, Tihomir, Šamanc, Horea, Trailović, Saša, Jeremić, Ivan, Nedeljković-Trailović, Jelena, "Cephapirine, povidone iodine and chlorhexidine digluconate in dairy cows endometritis treatment" in Zbornik naučnih radova Instituta PKB Agroekonomik, 18, no. 3-4 (2012):59-70,
https://hdl.handle.net/21.15107/rcub_veterinar_931 .