TY  - JOUR
AU  - Bruić, Marija
AU  - Pirković, Andrea
AU  - Borozan, Sunčica
AU  - Nacka Aleksić, Mirjana
AU  - Jovanović Krivokuća, Milica
AU  - Spremo-Potparević, Biljana
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3864
AB  - Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant secondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing
these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of
oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory
cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 µM and 100 µM
concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content,
extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant enzymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along
with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed antiinflammatory activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and antiinflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing potential as a therapeutic agent in preventing adverse pregnancy outcomes.
PB  - Elsevier
T2  - Reproductive Toxicology
T1  - Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line
VL  - 126
SP  - 108585
DO  - 10.1016/j.reprotox.2024.108585
ER  - 

@article{
author = "Bruić, Marija and Pirković, Andrea and Borozan, Sunčica and Nacka Aleksić, Mirjana and Jovanović Krivokuća, Milica and Spremo-Potparević, Biljana",
year = "2024",
abstract = "Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant secondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing
these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of
oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory
cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 µM and 100 µM
concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content,
extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant enzymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along
with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed antiinflammatory activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and antiinflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing potential as a therapeutic agent in preventing adverse pregnancy outcomes.",
publisher = "Elsevier",
journal = "Reproductive Toxicology",
title = "Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line",
volume = "126",
pages = "108585",
doi = "10.1016/j.reprotox.2024.108585"
}

Bruić, M., Pirković, A., Borozan, S., Nacka Aleksić, M., Jovanović Krivokuća, M.,& Spremo-Potparević, B.. (2024). Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line. in Reproductive Toxicology
Elsevier., 126, 108585.
https://doi.org/10.1016/j.reprotox.2024.108585

Bruić M, Pirković A, Borozan S, Nacka Aleksić M, Jovanović Krivokuća M, Spremo-Potparević B. Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line. in Reproductive Toxicology. 2024;126:108585.
doi:10.1016/j.reprotox.2024.108585 .

Bruić, Marija, Pirković, Andrea, Borozan, Sunčica, Nacka Aleksić, Mirjana, Jovanović Krivokuća, Milica, Spremo-Potparević, Biljana, "Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line" in Reproductive Toxicology, 126 (2024):108585,
https://doi.org/10.1016/j.reprotox.2024.108585 . .

TY  - JOUR
AU  - Vasilev, Saša
AU  - Mitić, Ivana
AU  - Mirilović, Milorad
AU  - Plavša, Dragana
AU  - Milakara, Emina
AU  - Plavšić, Budimir
AU  - Sofronić-Milosavljević, Ljiljana
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2541
AB  - In Serbia, modern pork production systems with implemented control measures, including
the detection of Trichinella larvae in meat (ISO18743), have eliminated farmed pork from
pigs slaughtered at abattoirs as a source of trichinellosis. Epidemiological data from 2011 to
2020 indicate that the number of human cases and the number of infected domestic pigs
has decreased significantly. Over the years, pork was the most frequent source of human
infection. Cases generally occurred in small family outbreaks, and the infection was linked
to consumption of raw or undercooked pork from backyard pigs. In most of the outbreaks,
T. spiralis was the aetiological agent of infection, but in 2016, a large outbreak was caused
by consumption of uninspected wild boar meat containing T. britovi larvae. To achieve safe
pork, it is important that consumers of pork from animals raised in backyard smallholdings
and of wild game meat are properly educated about the risks associated with consumption of
untested meat. Laboratories conducting Trichinella testing should have a functional quality
assurance system to ensure competency of analysts and that accurate and repeatable results
are achieved. Regular participation in proficiency testing is needed.
PB  - Cambridge University Press
T2  - Epidemiology and Infection
T1  - Trichinella infection in Serbia from 2011 to 2020: a success story in the field of One Health
VL  - 151
VL  - 8
SP  - 20e
DO  - 10.1017/S0950268823000109
ER  - 

@article{
author = "Vasilev, Saša and Mitić, Ivana and Mirilović, Milorad and Plavša, Dragana and Milakara, Emina and Plavšić, Budimir and Sofronić-Milosavljević, Ljiljana",
year = "2023",
abstract = "In Serbia, modern pork production systems with implemented control measures, including
the detection of Trichinella larvae in meat (ISO18743), have eliminated farmed pork from
pigs slaughtered at abattoirs as a source of trichinellosis. Epidemiological data from 2011 to
2020 indicate that the number of human cases and the number of infected domestic pigs
has decreased significantly. Over the years, pork was the most frequent source of human
infection. Cases generally occurred in small family outbreaks, and the infection was linked
to consumption of raw or undercooked pork from backyard pigs. In most of the outbreaks,
T. spiralis was the aetiological agent of infection, but in 2016, a large outbreak was caused
by consumption of uninspected wild boar meat containing T. britovi larvae. To achieve safe
pork, it is important that consumers of pork from animals raised in backyard smallholdings
and of wild game meat are properly educated about the risks associated with consumption of
untested meat. Laboratories conducting Trichinella testing should have a functional quality
assurance system to ensure competency of analysts and that accurate and repeatable results
are achieved. Regular participation in proficiency testing is needed.",
publisher = "Cambridge University Press",
journal = "Epidemiology and Infection",
title = "Trichinella infection in Serbia from 2011 to 2020: a success story in the field of One Health",
volume = "151, 8",
pages = "20e",
doi = "10.1017/S0950268823000109"
}

Vasilev, S., Mitić, I., Mirilović, M., Plavša, D., Milakara, E., Plavšić, B.,& Sofronić-Milosavljević, L.. (2023). Trichinella infection in Serbia from 2011 to 2020: a success story in the field of One Health. in Epidemiology and Infection
Cambridge University Press., 151, 20e.
https://doi.org/10.1017/S0950268823000109

Vasilev S, Mitić I, Mirilović M, Plavša D, Milakara E, Plavšić B, Sofronić-Milosavljević L. Trichinella infection in Serbia from 2011 to 2020: a success story in the field of One Health. in Epidemiology and Infection. 2023;151:20e.
doi:10.1017/S0950268823000109 .

Vasilev, Saša, Mitić, Ivana, Mirilović, Milorad, Plavša, Dragana, Milakara, Emina, Plavšić, Budimir, Sofronić-Milosavljević, Ljiljana, "Trichinella infection in Serbia from 2011 to 2020: a success story in the field of One Health" in Epidemiology and Infection, 151 (2023):20e,
https://doi.org/10.1017/S0950268823000109 . .

TY  - JOUR
AU  - Pirković, Andrea
AU  - Vilotić, Aleksandra
AU  - Borozan, Sunčica
AU  - Nacka-Aleksić, Mirjana
AU  - Bojić-Trbojević, Žanka
AU  - Jovanović-Krivokuća, Milica
AU  - Battino, Maurizio
AU  - Giampieri, Francesca
AU  - Dekanski, Dragana
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2539
AB  - Olive-derived bioactive compound oleuropein was evaluated against damage induced by hydrogen peroxide in human trophoblast cells in vitro, by examining the changes in several markers implicated in oxidative stress interactions in the placenta. Trophoblast HTR-8/SVneo cells were preincubated with OLE at 10 and 100 µM and exposed to H2O2, as a model of oxidative stress. Protein and lipid peroxidation, as well as antioxidant enzymes’ activity, were determined spectrophotometrically, and DNA damage was evaluated by comet assay. iNOS protein expression was assessed by Western blot, while the mRNA expression of pro- and anti-apoptotic genes BAX and BCL2 and transcription factor NFE2L2, as well as cytokines IL-6 and TNF α were determined by qPCR. Oleuropein demonstrated cytoprotective effects against H2O2 in trophoblast cells by significantly improving the antioxidant status and preventing protein and lipid damage, as well as reducing the iNOS levels. OLE reduced the mRNA expression of IL-6 and TNF α, however, it did not influence the expression of NFE2L2 or the BAX/BCL2 ratio after H2O2 exposure. Oleuropein per se did not lead to any adverse effects in HTR-8/SVneo cells under the described conditions, confirming its safety in vitro. In conclusion, it significantly attenuated oxidative damage and restored antioxidant functioning, confirming its protective role in trophoblast. © 2023 by the authors.
PB  - MDPI
T2  - Antioxidants
T1  - Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells
VL  - 12
VL  - 1
SP  - 197
DO  - 10.3390/antiox12010197
ER  - 

@article{
author = "Pirković, Andrea and Vilotić, Aleksandra and Borozan, Sunčica and Nacka-Aleksić, Mirjana and Bojić-Trbojević, Žanka and Jovanović-Krivokuća, Milica and Battino, Maurizio and Giampieri, Francesca and Dekanski, Dragana",
year = "2023",
abstract = "Olive-derived bioactive compound oleuropein was evaluated against damage induced by hydrogen peroxide in human trophoblast cells in vitro, by examining the changes in several markers implicated in oxidative stress interactions in the placenta. Trophoblast HTR-8/SVneo cells were preincubated with OLE at 10 and 100 µM and exposed to H2O2, as a model of oxidative stress. Protein and lipid peroxidation, as well as antioxidant enzymes’ activity, were determined spectrophotometrically, and DNA damage was evaluated by comet assay. iNOS protein expression was assessed by Western blot, while the mRNA expression of pro- and anti-apoptotic genes BAX and BCL2 and transcription factor NFE2L2, as well as cytokines IL-6 and TNF α were determined by qPCR. Oleuropein demonstrated cytoprotective effects against H2O2 in trophoblast cells by significantly improving the antioxidant status and preventing protein and lipid damage, as well as reducing the iNOS levels. OLE reduced the mRNA expression of IL-6 and TNF α, however, it did not influence the expression of NFE2L2 or the BAX/BCL2 ratio after H2O2 exposure. Oleuropein per se did not lead to any adverse effects in HTR-8/SVneo cells under the described conditions, confirming its safety in vitro. In conclusion, it significantly attenuated oxidative damage and restored antioxidant functioning, confirming its protective role in trophoblast. © 2023 by the authors.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells",
volume = "12, 1",
pages = "197",
doi = "10.3390/antiox12010197"
}

Pirković, A., Vilotić, A., Borozan, S., Nacka-Aleksić, M., Bojić-Trbojević, Ž., Jovanović-Krivokuća, M., Battino, M., Giampieri, F.,& Dekanski, D.. (2023). Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells. in Antioxidants
MDPI., 12, 197.
https://doi.org/10.3390/antiox12010197

Pirković A, Vilotić A, Borozan S, Nacka-Aleksić M, Bojić-Trbojević Ž, Jovanović-Krivokuća M, Battino M, Giampieri F, Dekanski D. Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells. in Antioxidants. 2023;12:197.
doi:10.3390/antiox12010197 .

Pirković, Andrea, Vilotić, Aleksandra, Borozan, Sunčica, Nacka-Aleksić, Mirjana, Bojić-Trbojević, Žanka, Jovanović-Krivokuća, Milica, Battino, Maurizio, Giampieri, Francesca, Dekanski, Dragana, "Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells" in Antioxidants, 12 (2023):197,
https://doi.org/10.3390/antiox12010197 . .

TY  - JOUR
AU  - Vasilev, Saša
AU  - Ciupescu, Laurentiu Mihai
AU  - Lalkovski, Nikolay
AU  - Balic, Davor
AU  - Vasilev, Dragan
AU  - Marucci, Gianluca
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3134
AB  - In some Southeastern European (SEE) countries, like Serbia, Romania, and Bulgaria, trichinellosis is one of the
most important foodborne zoonotic diseases. In those countries, EU regulation and local authorities require the
staff of laboratories performing official controls on meat to be properly trained and to check their competence by
participating regularly in proficiency testing (PT). PTs are organized by National Reference Laboratories for
Trichinella of each country and involve all official laboratories testing meat. In Romania and Bulgaria, the organization
of PT for the detection of Trichinella larvae in meat by Magnetic Stirrer Method (MSM) started in
2012. In Croatia and Serbia PT was first organized in 2015 and 2017, respectively. This study presents data on
the performance obtained by official laboratories of SEE countries that organize PT at national level and compares
the performance obtained by laboratories belonging to different countries. Results suggest that the constant
participation in PT leads to an increase in the performance of participating laboratories by positively affecting
the staff accuracy in sample testing by MSM. Since the percentage of recovered larvae was in some cases suboptimal
(<80%) and occasionally very poor (<40%), there is room for improvement. The regular participation in
PT by laboratories involved in official controls on meat intended for human consumption is fundamental to
guarantee consumer safety.
PB  - Elsevier
T2  - Veterinary Parasitology
T1  - Trichinella proficiency testing in Southeastern European countries
VL  - 320
SP  - 109982
DO  - 10.1016/j.vetpar.2023.109982
ER  - 

@article{
author = "Vasilev, Saša and Ciupescu, Laurentiu Mihai and Lalkovski, Nikolay and Balic, Davor and Vasilev, Dragan and Marucci, Gianluca",
year = "2023",
abstract = "In some Southeastern European (SEE) countries, like Serbia, Romania, and Bulgaria, trichinellosis is one of the
most important foodborne zoonotic diseases. In those countries, EU regulation and local authorities require the
staff of laboratories performing official controls on meat to be properly trained and to check their competence by
participating regularly in proficiency testing (PT). PTs are organized by National Reference Laboratories for
Trichinella of each country and involve all official laboratories testing meat. In Romania and Bulgaria, the organization
of PT for the detection of Trichinella larvae in meat by Magnetic Stirrer Method (MSM) started in
2012. In Croatia and Serbia PT was first organized in 2015 and 2017, respectively. This study presents data on
the performance obtained by official laboratories of SEE countries that organize PT at national level and compares
the performance obtained by laboratories belonging to different countries. Results suggest that the constant
participation in PT leads to an increase in the performance of participating laboratories by positively affecting
the staff accuracy in sample testing by MSM. Since the percentage of recovered larvae was in some cases suboptimal
(<80%) and occasionally very poor (<40%), there is room for improvement. The regular participation in
PT by laboratories involved in official controls on meat intended for human consumption is fundamental to
guarantee consumer safety.",
publisher = "Elsevier",
journal = "Veterinary Parasitology",
title = "Trichinella proficiency testing in Southeastern European countries",
volume = "320",
pages = "109982",
doi = "10.1016/j.vetpar.2023.109982"
}

Vasilev, S., Ciupescu, L. M., Lalkovski, N., Balic, D., Vasilev, D.,& Marucci, G.. (2023). Trichinella proficiency testing in Southeastern European countries. in Veterinary Parasitology
Elsevier., 320, 109982.
https://doi.org/10.1016/j.vetpar.2023.109982

Vasilev S, Ciupescu LM, Lalkovski N, Balic D, Vasilev D, Marucci G. Trichinella proficiency testing in Southeastern European countries. in Veterinary Parasitology. 2023;320:109982.
doi:10.1016/j.vetpar.2023.109982 .

Vasilev, Saša, Ciupescu, Laurentiu Mihai, Lalkovski, Nikolay, Balic, Davor, Vasilev, Dragan, Marucci, Gianluca, "Trichinella proficiency testing in Southeastern European countries" in Veterinary Parasitology, 320 (2023):109982,
https://doi.org/10.1016/j.vetpar.2023.109982 . .

TY  - CONF
AU  - Vasilev, Saša
AU  - Mirilović, Milorad
AU  - Ignjatović, Marija
AU  - Milakara, Emina
AU  - Mitić, Ivana
AU  - Plavšić, Budimir
AU  - Plavša, Dragana
AU  - Bošković, Tamara
AU  - Vasilev, Dragan
AU  - Karabasil, Nedjeljko
AU  - Sabljić, Ljiljana
AU  - Ilić, Nataša
AU  - Živojinović, Milena
AU  - Sofronić Milosavljević, Ljiljana
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2486
AB  - U Srbiji trihineloza je jedna od najznačajnijih zoonoza koje se prenose hranom. Prvi izveštaj o nalazu kod svinja je iz 1918. godine, a prvi slučaj kod ljudi 1923. godine u Zemunu. Detekcija prisustva trihinele je prvobitno vršena trihineloskopijom. Od 1984. uvedena je veštačka digestija. Savremeni sistemi proizvodnje svinjskog mesa, mere kontrole, metoda veštačke digestije eliminisali su meso svinja sa velikih farmi kao izvor trihineloze. Prvi put u Srbiji Nacionalna referentna laboratorija za trihinelozu (NRLT) organizovala je 2017. godine kontrolu kvaliteta rada za detekciju larvi trihinele u mesu metodom magnetne mešalice (MSM). Svi učesnici (veterinarski instituti) uspešno su prošli testiranje. Članovi tima NRLT napravili su (2019. godine) novi cELISA test za otkrivanje infekcije trihinelom kod životinja i ljudi i Svetska komisija za trihinelozu ga je prepoznala kao koristan za međunarodnu upotrebu. Epidemiološki podaci iz poslednjih decenija ukazuju da je broj obolelih ljudi kao i broj zaraženih životinja značajno smanjen. Svinjsko meso je godinama najčešći izvor infekcije ljudi u Srbiji. Slučajevi se javljaju u porodičnim epidemijama i rizik je povezan sa konzumacijom svinjskog mesa u kome se nalaze larve Trichinella, a uzgajaju se bez primene higijenskih pravila i životinje nisu veterinarski testirane (epidemija u Obrenovcu i Parizu, Francuska). U većini slučajeva T. spiralis je bila etiološki agens infekcije, ali smo u 2016. godini imali veliku epidemiju izazvanu konzumacijom mesa divljih svinja koje je sadržalo larve T britovi (Čajetina, 123 osobe). Važno je: 1. Lovce i potrošače mesa svinja i mesa divljači u Srbiji edukovati o riziku u vezi sa konzumiranjem neproverenog mesa. 2. Kontrola sistema kvaliteta kod veterinarskih subjekata koji rade testiranje na prisustvo larvi trihinele u uzorcima mesa, kao i redovno učešće u PT.
AB  - In Serbia Trichinellosis is one of the most important foodborne zoonotic diseases. The first report of swine infection was in 1918 and first human case was in 1923. From 1984 artificial digestion was adopted for use in preventing human trichinellosis. Modern pork production systems, implemented control measures, artificial digestion method have eliminated farm pork as a source for trichinellosis. For the first time in Serbia National Reference Laboratory for trichinellosis (NRLT) organized in 2017 proficiency test (PT) for the detection of Trichinella larvae in meat by Magnetic Stirrer Method (MSM). All participants (veterinary institutes) successfully passed the testing. Members of NRLT team made (in 2019) new cELISA test for detection of Trichinella infection in animals and humans and the ICT recognized the test as useful for international use. Epidemiological data from the last decades indicate that the number of human cases as well as the number of infected animals has decreased significantly. Over the years, pork was the most frequent source of human trichinellosis. Cases generally occurred in family outbreaks and risk is linked to untested backyard pork consumption. Meat and meat products offered to relatives and friends may be source of infection with Trichinella when backyard pigs are raised without any compliance with hygienic rules and animals are not veterinary tested. In most numbers of outbreaks T. spiralis were the etiological agent of infection but in 2016 we had a large outbreak provoked by consumption of wild boar meat containing T britovi larvae. It is important that: 1. Hunters and consumers of backyard pigs and wild game meat should be educated about the risk associated with consumption of untested meat. 2. Control of Trichinella QA system in veterinary subjects (testing for Trichinella presence in meat samples) and regularly participation in PTs are needed to achieve safe food for consumers.
PB  - Veterinarska komora Republike Srpske
C3  - 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina)
T1  - Trichinella infection in Serbia
T1  - Infekcija trihinelom u Srbiji
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2486
ER  - 

@conference{
author = "Vasilev, Saša and Mirilović, Milorad and Ignjatović, Marija and Milakara, Emina and Mitić, Ivana and Plavšić, Budimir and Plavša, Dragana and Bošković, Tamara and Vasilev, Dragan and Karabasil, Nedjeljko and Sabljić, Ljiljana and Ilić, Nataša and Živojinović, Milena and Sofronić Milosavljević, Ljiljana",
year = "2022",
abstract = "U Srbiji trihineloza je jedna od najznačajnijih zoonoza koje se prenose hranom. Prvi izveštaj o nalazu kod svinja je iz 1918. godine, a prvi slučaj kod ljudi 1923. godine u Zemunu. Detekcija prisustva trihinele je prvobitno vršena trihineloskopijom. Od 1984. uvedena je veštačka digestija. Savremeni sistemi proizvodnje svinjskog mesa, mere kontrole, metoda veštačke digestije eliminisali su meso svinja sa velikih farmi kao izvor trihineloze. Prvi put u Srbiji Nacionalna referentna laboratorija za trihinelozu (NRLT) organizovala je 2017. godine kontrolu kvaliteta rada za detekciju larvi trihinele u mesu metodom magnetne mešalice (MSM). Svi učesnici (veterinarski instituti) uspešno su prošli testiranje. Članovi tima NRLT napravili su (2019. godine) novi cELISA test za otkrivanje infekcije trihinelom kod životinja i ljudi i Svetska komisija za trihinelozu ga je prepoznala kao koristan za međunarodnu upotrebu. Epidemiološki podaci iz poslednjih decenija ukazuju da je broj obolelih ljudi kao i broj zaraženih životinja značajno smanjen. Svinjsko meso je godinama najčešći izvor infekcije ljudi u Srbiji. Slučajevi se javljaju u porodičnim epidemijama i rizik je povezan sa konzumacijom svinjskog mesa u kome se nalaze larve Trichinella, a uzgajaju se bez primene higijenskih pravila i životinje nisu veterinarski testirane (epidemija u Obrenovcu i Parizu, Francuska). U većini slučajeva T. spiralis je bila etiološki agens infekcije, ali smo u 2016. godini imali veliku epidemiju izazvanu konzumacijom mesa divljih svinja koje je sadržalo larve T britovi (Čajetina, 123 osobe). Važno je: 1. Lovce i potrošače mesa svinja i mesa divljači u Srbiji edukovati o riziku u vezi sa konzumiranjem neproverenog mesa. 2. Kontrola sistema kvaliteta kod veterinarskih subjekata koji rade testiranje na prisustvo larvi trihinele u uzorcima mesa, kao i redovno učešće u PT., In Serbia Trichinellosis is one of the most important foodborne zoonotic diseases. The first report of swine infection was in 1918 and first human case was in 1923. From 1984 artificial digestion was adopted for use in preventing human trichinellosis. Modern pork production systems, implemented control measures, artificial digestion method have eliminated farm pork as a source for trichinellosis. For the first time in Serbia National Reference Laboratory for trichinellosis (NRLT) organized in 2017 proficiency test (PT) for the detection of Trichinella larvae in meat by Magnetic Stirrer Method (MSM). All participants (veterinary institutes) successfully passed the testing. Members of NRLT team made (in 2019) new cELISA test for detection of Trichinella infection in animals and humans and the ICT recognized the test as useful for international use. Epidemiological data from the last decades indicate that the number of human cases as well as the number of infected animals has decreased significantly. Over the years, pork was the most frequent source of human trichinellosis. Cases generally occurred in family outbreaks and risk is linked to untested backyard pork consumption. Meat and meat products offered to relatives and friends may be source of infection with Trichinella when backyard pigs are raised without any compliance with hygienic rules and animals are not veterinary tested. In most numbers of outbreaks T. spiralis were the etiological agent of infection but in 2016 we had a large outbreak provoked by consumption of wild boar meat containing T britovi larvae. It is important that: 1. Hunters and consumers of backyard pigs and wild game meat should be educated about the risk associated with consumption of untested meat. 2. Control of Trichinella QA system in veterinary subjects (testing for Trichinella presence in meat samples) and regularly participation in PTs are needed to achieve safe food for consumers.",
publisher = "Veterinarska komora Republike Srpske",
journal = "27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina)",
title = "Trichinella infection in Serbia, Infekcija trihinelom u Srbiji",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2486"
}

Vasilev, S., Mirilović, M., Ignjatović, M., Milakara, E., Mitić, I., Plavšić, B., Plavša, D., Bošković, T., Vasilev, D., Karabasil, N., Sabljić, L., Ilić, N., Živojinović, M.,& Sofronić Milosavljević, L.. (2022). Trichinella infection in Serbia. in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina)
Veterinarska komora Republike Srpske..
https://hdl.handle.net/21.15107/rcub_veterinar_2486

Vasilev S, Mirilović M, Ignjatović M, Milakara E, Mitić I, Plavšić B, Plavša D, Bošković T, Vasilev D, Karabasil N, Sabljić L, Ilić N, Živojinović M, Sofronić Milosavljević L. Trichinella infection in Serbia. in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina). 2022;.
https://hdl.handle.net/21.15107/rcub_veterinar_2486 .

Vasilev, Saša, Mirilović, Milorad, Ignjatović, Marija, Milakara, Emina, Mitić, Ivana, Plavšić, Budimir, Plavša, Dragana, Bošković, Tamara, Vasilev, Dragan, Karabasil, Nedjeljko, Sabljić, Ljiljana, Ilić, Nataša, Živojinović, Milena, Sofronić Milosavljević, Ljiljana, "Trichinella infection in Serbia" in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) (2022),
https://hdl.handle.net/21.15107/rcub_veterinar_2486 .

TY  - JOUR
AU  - Kostić, Sanja
AU  - Vilotić, Aleksandra
AU  - Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Borozan, Sunčica
AU  - Nacka-Aleksić, Mirjana
AU  - Vrzić-Petronijević, Svetlana
AU  - Jovanović Krivokuća, Milica
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2842
AB  - Caffeic acid is highlighted as one of the major phenolic compounds present in foods with known antioxidant activity. This phenolic is among commonly consumed substances in everyday diet of pregnant women. However, there is not enough information on its effects during pregnancy, especially the most vulnerable early stage. Extravillous trophoblast cells are specific cells of the placenta that come in direct contact with maternal uterine tissue. Through this study we investigated the cytoprotective effects of caffeic acid on H2O2-induced oxidative damage in first trimester extravillous trophoblast cell line HTR-8/SVneo. Investigated concentrations (1–100 μM) of caffeic acid showed neither cytotoxic nor genotoxic effects on HTR-8/SVneo cells. The treatment with caffeic acid 100 μM significantly increased the percentage of cells in G2/M phase of the cell cycle, compared to non-treated cells. Pretreatment with caffeic acid (10 and 100 μM) attenuated oxidative DNA damage significantly, reduced cytotoxicity, protein and lipid peroxidation, and restored antioxidant capacity in trophoblast cells following H2O2 exposure. This beneficial outcome is probably mediated by the augmentation of GSH and effective ROS scavenging by caffeic acid. These promising results require further investigations to reveal the additional mechanisms/pathways and confirmation through studies in vivo.
PB  - Elsevier
T2  - Food and Chemical Toxicology
T1  - Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity
VL  - 163
SP  - 112993
DO  - 10.1016/j.fct.2022.112993
ER  - 

@article{
author = "Kostić, Sanja and Vilotić, Aleksandra and Pirković, Andrea and Dekanski, Dragana and Borozan, Sunčica and Nacka-Aleksić, Mirjana and Vrzić-Petronijević, Svetlana and Jovanović Krivokuća, Milica",
year = "2022",
abstract = "Caffeic acid is highlighted as one of the major phenolic compounds present in foods with known antioxidant activity. This phenolic is among commonly consumed substances in everyday diet of pregnant women. However, there is not enough information on its effects during pregnancy, especially the most vulnerable early stage. Extravillous trophoblast cells are specific cells of the placenta that come in direct contact with maternal uterine tissue. Through this study we investigated the cytoprotective effects of caffeic acid on H2O2-induced oxidative damage in first trimester extravillous trophoblast cell line HTR-8/SVneo. Investigated concentrations (1–100 μM) of caffeic acid showed neither cytotoxic nor genotoxic effects on HTR-8/SVneo cells. The treatment with caffeic acid 100 μM significantly increased the percentage of cells in G2/M phase of the cell cycle, compared to non-treated cells. Pretreatment with caffeic acid (10 and 100 μM) attenuated oxidative DNA damage significantly, reduced cytotoxicity, protein and lipid peroxidation, and restored antioxidant capacity in trophoblast cells following H2O2 exposure. This beneficial outcome is probably mediated by the augmentation of GSH and effective ROS scavenging by caffeic acid. These promising results require further investigations to reveal the additional mechanisms/pathways and confirmation through studies in vivo.",
publisher = "Elsevier",
journal = "Food and Chemical Toxicology",
title = "Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity",
volume = "163",
pages = "112993",
doi = "10.1016/j.fct.2022.112993"
}

Kostić, S., Vilotić, A., Pirković, A., Dekanski, D., Borozan, S., Nacka-Aleksić, M., Vrzić-Petronijević, S.,& Jovanović Krivokuća, M.. (2022). Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity. in Food and Chemical Toxicology
Elsevier., 163, 112993.
https://doi.org/10.1016/j.fct.2022.112993

Kostić S, Vilotić A, Pirković A, Dekanski D, Borozan S, Nacka-Aleksić M, Vrzić-Petronijević S, Jovanović Krivokuća M. Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity. in Food and Chemical Toxicology. 2022;163:112993.
doi:10.1016/j.fct.2022.112993 .

Kostić, Sanja, Vilotić, Aleksandra, Pirković, Andrea, Dekanski, Dragana, Borozan, Sunčica, Nacka-Aleksić, Mirjana, Vrzić-Petronijević, Svetlana, Jovanović Krivokuća, Milica, "Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity" in Food and Chemical Toxicology, 163 (2022):112993,
https://doi.org/10.1016/j.fct.2022.112993 . .

TY  - JOUR
AU  - Vasilev, S.
AU  - Suvajdžić, Branko
AU  - Mitic, I.
AU  - Sabljić, Lj.
AU  - Karabasil, Nedjeljko
AU  - Sofronić Milosavljević, Lj.
AU  - Vasilev, Dragan
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2495
AB  - Trichinellosis is an important worldwide foodborne zoonosis. The gold standard test to detect Trichinella spp. larvae in muscle samples of animals intended for human consumption is the artificial digestion method. Handling and dispensing of conventional pepsin powder present significant safety risks for analysts. The use of pepsin powder that is resistant to aerosolization should alleviate these safety concerns. The aim of this study was to compare the efficacy of an aerosol-resistant pepsin powder to conventional pepsin powder in the artificial digestion method. Proficiency samples of pork diaphragm containing specific numbers of viable Trichinella spiralis larvae were tested in two laboratories. The results revealed that aerosol-resistant pepsin was simple, effective and convenient to use, and showed good solubility and larval recovery that met the requirements of the European Union regulation EU 2015/1375. Overall, the efficacy of the aerosol-resistant pepsin was comparable to the conventional pepsin and safer for analysts.
PB  - Cambridge University Press
T2  - Journal of Helminthology
T1  - Efficacy of an aerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat
VL  - 96
SP  - e71
DO  - 10.1017/S0022149X2200061X
ER  - 

@article{
author = "Vasilev, S. and Suvajdžić, Branko and Mitic, I. and Sabljić, Lj. and Karabasil, Nedjeljko and Sofronić Milosavljević, Lj. and Vasilev, Dragan",
year = "2022",
abstract = "Trichinellosis is an important worldwide foodborne zoonosis. The gold standard test to detect Trichinella spp. larvae in muscle samples of animals intended for human consumption is the artificial digestion method. Handling and dispensing of conventional pepsin powder present significant safety risks for analysts. The use of pepsin powder that is resistant to aerosolization should alleviate these safety concerns. The aim of this study was to compare the efficacy of an aerosol-resistant pepsin powder to conventional pepsin powder in the artificial digestion method. Proficiency samples of pork diaphragm containing specific numbers of viable Trichinella spiralis larvae were tested in two laboratories. The results revealed that aerosol-resistant pepsin was simple, effective and convenient to use, and showed good solubility and larval recovery that met the requirements of the European Union regulation EU 2015/1375. Overall, the efficacy of the aerosol-resistant pepsin was comparable to the conventional pepsin and safer for analysts.",
publisher = "Cambridge University Press",
journal = "Journal of Helminthology",
title = "Efficacy of an aerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat",
volume = "96",
pages = "e71",
doi = "10.1017/S0022149X2200061X"
}

Vasilev, S., Suvajdžić, B., Mitic, I., Sabljić, Lj., Karabasil, N., Sofronić Milosavljević, Lj.,& Vasilev, D.. (2022). Efficacy of an aerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat. in Journal of Helminthology
Cambridge University Press., 96, e71.
https://doi.org/10.1017/S0022149X2200061X

Vasilev S, Suvajdžić B, Mitic I, Sabljić L, Karabasil N, Sofronić Milosavljević L, Vasilev D. Efficacy of an aerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat. in Journal of Helminthology. 2022;96:e71.
doi:10.1017/S0022149X2200061X .

Vasilev, S., Suvajdžić, Branko, Mitic, I., Sabljić, Lj., Karabasil, Nedjeljko, Sofronić Milosavljević, Lj., Vasilev, Dragan, "Efficacy of an aerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat" in Journal of Helminthology, 96 (2022):e71,
https://doi.org/10.1017/S0022149X2200061X . .