Fatty Acid Composition and Oxidative Stress Parameters in Plasma after Fish Oil Supplementation in Aging
2014
Аутори
Popović, TamaraBorozan, Sunčica
Takić, Marija M.
Kojadinović, Milica J.
Ranković, Slavica
Ranić, Marija
de Luka, Silvio
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Fish oil affects oxidative stress parameters and changes in phospholipids fatty acids profiles in plasma, erythrocytes and tissues. We examined the effects of fish oil supplementation in young and old male Wistar rats (3 and 22 months old) on plasma phospholipids fatty acids profiles and blood oxidative stress parameters. Twenty young and twenty aged Wistar rats were randomly divided into four groups (ten animals each): two control groups and two supplemented groups treated for 6 weeks with fish oil capsules containing 45 mg eicosapentanoic acid (EPA) and 30 mg docosahexanoic acid (DHA). Fish oil supplementation changed the percentage of long chain fatty acids (FAs): the elevated percentage of eicosatrienoic acid (ETA, 20:3), eicosapentanoic (EPA, 20:5), docosapentanoic acid (DPA, 22:5), n-3 fatty acids and decreased arachidonic acid (AA, 20:4). However, there were no age-related changes in total SH groups, the percentages of palmitic (16:0), palmitoleic (16:1), oleic (18:1 (n-9)) and ...linoleic acid (18:2) in plasma phospholipids and MUFA and they were neither reversed nor prevented by fish oil supplementation. Results showed that fish oil supplementation increased SOD and CAT activities in erythrocytes, and PON activity in the blood plasma of both young and aged rats. Furthermore, fish oil supplementation decreased lipid peroxidation (MDA) and nitrite levels in both young and aged rats implying better antioxidant protection and a lower level of oxidative pressure after fish oil supplementation. Our results suggest that fish oil supplementation is beneficial regarding better antioxidant protection in both young and aged rats, while applied treatment differs in plasma phospholipids FAs composition.
Кључне речи:
fish oil / aged rats / oxidative stress / fatty acidsИзвор:
Croatica Chemica Acta, 2014, 87, 3, 207-212Издавач:
- Croatian Chemical Soc, Zagreb
Финансирање / пројекти:
- Биолошки механизми, нутритивни унос и статус полинезасићених масних киселина и фолата: Унапређење исхране у Србији (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41030)
- Аберације ћелијског циклуса и утицај оксидативног стреса на неуродегенеративне процесе и малигну трансформацију ћелије (RS-MESTD-Basic Research (BR or ON)-173034)
DOI: 10.5562/cca2405
ISSN: 0011-1643
WoS: 000348414000003
Scopus: 2-s2.0-84919709714
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Popović, Tamara AU - Borozan, Sunčica AU - Takić, Marija M. AU - Kojadinović, Milica J. AU - Ranković, Slavica AU - Ranić, Marija AU - de Luka, Silvio PY - 2014 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1095 AB - Fish oil affects oxidative stress parameters and changes in phospholipids fatty acids profiles in plasma, erythrocytes and tissues. We examined the effects of fish oil supplementation in young and old male Wistar rats (3 and 22 months old) on plasma phospholipids fatty acids profiles and blood oxidative stress parameters. Twenty young and twenty aged Wistar rats were randomly divided into four groups (ten animals each): two control groups and two supplemented groups treated for 6 weeks with fish oil capsules containing 45 mg eicosapentanoic acid (EPA) and 30 mg docosahexanoic acid (DHA). Fish oil supplementation changed the percentage of long chain fatty acids (FAs): the elevated percentage of eicosatrienoic acid (ETA, 20:3), eicosapentanoic (EPA, 20:5), docosapentanoic acid (DPA, 22:5), n-3 fatty acids and decreased arachidonic acid (AA, 20:4). However, there were no age-related changes in total SH groups, the percentages of palmitic (16:0), palmitoleic (16:1), oleic (18:1 (n-9)) and linoleic acid (18:2) in plasma phospholipids and MUFA and they were neither reversed nor prevented by fish oil supplementation. Results showed that fish oil supplementation increased SOD and CAT activities in erythrocytes, and PON activity in the blood plasma of both young and aged rats. Furthermore, fish oil supplementation decreased lipid peroxidation (MDA) and nitrite levels in both young and aged rats implying better antioxidant protection and a lower level of oxidative pressure after fish oil supplementation. Our results suggest that fish oil supplementation is beneficial regarding better antioxidant protection in both young and aged rats, while applied treatment differs in plasma phospholipids FAs composition. PB - Croatian Chemical Soc, Zagreb T2 - Croatica Chemica Acta T1 - Fatty Acid Composition and Oxidative Stress Parameters in Plasma after Fish Oil Supplementation in Aging VL - 87 IS - 3 SP - 207 EP - 212 DO - 10.5562/cca2405 ER -
@article{ author = "Popović, Tamara and Borozan, Sunčica and Takić, Marija M. and Kojadinović, Milica J. and Ranković, Slavica and Ranić, Marija and de Luka, Silvio", year = "2014", abstract = "Fish oil affects oxidative stress parameters and changes in phospholipids fatty acids profiles in plasma, erythrocytes and tissues. We examined the effects of fish oil supplementation in young and old male Wistar rats (3 and 22 months old) on plasma phospholipids fatty acids profiles and blood oxidative stress parameters. Twenty young and twenty aged Wistar rats were randomly divided into four groups (ten animals each): two control groups and two supplemented groups treated for 6 weeks with fish oil capsules containing 45 mg eicosapentanoic acid (EPA) and 30 mg docosahexanoic acid (DHA). Fish oil supplementation changed the percentage of long chain fatty acids (FAs): the elevated percentage of eicosatrienoic acid (ETA, 20:3), eicosapentanoic (EPA, 20:5), docosapentanoic acid (DPA, 22:5), n-3 fatty acids and decreased arachidonic acid (AA, 20:4). However, there were no age-related changes in total SH groups, the percentages of palmitic (16:0), palmitoleic (16:1), oleic (18:1 (n-9)) and linoleic acid (18:2) in plasma phospholipids and MUFA and they were neither reversed nor prevented by fish oil supplementation. Results showed that fish oil supplementation increased SOD and CAT activities in erythrocytes, and PON activity in the blood plasma of both young and aged rats. Furthermore, fish oil supplementation decreased lipid peroxidation (MDA) and nitrite levels in both young and aged rats implying better antioxidant protection and a lower level of oxidative pressure after fish oil supplementation. Our results suggest that fish oil supplementation is beneficial regarding better antioxidant protection in both young and aged rats, while applied treatment differs in plasma phospholipids FAs composition.", publisher = "Croatian Chemical Soc, Zagreb", journal = "Croatica Chemica Acta", title = "Fatty Acid Composition and Oxidative Stress Parameters in Plasma after Fish Oil Supplementation in Aging", volume = "87", number = "3", pages = "207-212", doi = "10.5562/cca2405" }
Popović, T., Borozan, S., Takić, M. M., Kojadinović, M. J., Ranković, S., Ranić, M.,& de Luka, S.. (2014). Fatty Acid Composition and Oxidative Stress Parameters in Plasma after Fish Oil Supplementation in Aging. in Croatica Chemica Acta Croatian Chemical Soc, Zagreb., 87(3), 207-212. https://doi.org/10.5562/cca2405
Popović T, Borozan S, Takić MM, Kojadinović MJ, Ranković S, Ranić M, de Luka S. Fatty Acid Composition and Oxidative Stress Parameters in Plasma after Fish Oil Supplementation in Aging. in Croatica Chemica Acta. 2014;87(3):207-212. doi:10.5562/cca2405 .
Popović, Tamara, Borozan, Sunčica, Takić, Marija M., Kojadinović, Milica J., Ranković, Slavica, Ranić, Marija, de Luka, Silvio, "Fatty Acid Composition and Oxidative Stress Parameters in Plasma after Fish Oil Supplementation in Aging" in Croatica Chemica Acta, 87, no. 3 (2014):207-212, https://doi.org/10.5562/cca2405 . .